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Biotechnology Information gad67
Fluorescence in situ hybridization showing characteristic labeling patterns with the use of antisense cRNA probes for <t>GAD67</t> ( A ), VGluT1 ( B ), VGluT2 ( C ), and VGluT3 ( D ) mRNAs. Inset in D shows expression of VGluT3 mRNA in putative interneurons dispersed in the hippocampus. BLA, Basolateral amygdala; Cx, cerebral cortex; Hip, hippocampus; MHb, medial habenula; Re, reticular thalamic nucleus; Th, thalamus; VMH, ventromedial hypothalamic nucleus. Scale bars, 1 mm.
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Images

1) Product Images from "Three Types of Neurochemical Projection from the Bed Nucleus of the Stria Terminalis to the Ventral Tegmental Area in Adult Mice"

Article Title: Three Types of Neurochemical Projection from the Bed Nucleus of the Stria Terminalis to the Ventral Tegmental Area in Adult Mice

Journal: The Journal of Neuroscience

doi: 10.1523/JNEUROSCI.4057-12.2012

Fluorescence in situ hybridization showing characteristic labeling patterns with the use of antisense cRNA probes for GAD67 ( A ), VGluT1 ( B ), VGluT2 ( C ), and VGluT3 ( D ) mRNAs. Inset in D shows expression of VGluT3 mRNA in putative interneurons dispersed in the hippocampus. BLA, Basolateral amygdala; Cx, cerebral cortex; Hip, hippocampus; MHb, medial habenula; Re, reticular thalamic nucleus; Th, thalamus; VMH, ventromedial hypothalamic nucleus. Scale bars, 1 mm.
Figure Legend Snippet: Fluorescence in situ hybridization showing characteristic labeling patterns with the use of antisense cRNA probes for GAD67 ( A ), VGluT1 ( B ), VGluT2 ( C ), and VGluT3 ( D ) mRNAs. Inset in D shows expression of VGluT3 mRNA in putative interneurons dispersed in the hippocampus. BLA, Basolateral amygdala; Cx, cerebral cortex; Hip, hippocampus; MHb, medial habenula; Re, reticular thalamic nucleus; Th, thalamus; VMH, ventromedial hypothalamic nucleus. Scale bars, 1 mm.

Techniques Used: Fluorescence, In Situ Hybridization, Labeling, Expressing

Chromogenic in situ hybridization showing GAD67 mRNA expression throughout the rostrocaudal extent of the BST. A , Low-power image of a coronal section through the anterior division of the BST. B–E , High-power images from the anterior ( B–D ) to the posterior ( E ) division of the BST. C is an enlarged view of A . The BST is surrounded by the caudate–putamen (CP), lateral septum (LS), preoptic area (PO), and ventral pallidum (VP). The anterior division of the BST is subdivided into the dorsal ( d ) and ventral ( v ) parts by the anterior commissure (ac). Cx, Cerebral cortex; fx, fornix; ic, internal capsule; lv, lateral ventricle; OT, olfactory tubercle; sm, stria medullaris; st, stria terminalis. Scale bars: A , 1 mm; B–E , 200 μm.
Figure Legend Snippet: Chromogenic in situ hybridization showing GAD67 mRNA expression throughout the rostrocaudal extent of the BST. A , Low-power image of a coronal section through the anterior division of the BST. B–E , High-power images from the anterior ( B–D ) to the posterior ( E ) division of the BST. C is an enlarged view of A . The BST is surrounded by the caudate–putamen (CP), lateral septum (LS), preoptic area (PO), and ventral pallidum (VP). The anterior division of the BST is subdivided into the dorsal ( d ) and ventral ( v ) parts by the anterior commissure (ac). Cx, Cerebral cortex; fx, fornix; ic, internal capsule; lv, lateral ventricle; OT, olfactory tubercle; sm, stria medullaris; st, stria terminalis. Scale bars: A , 1 mm; B–E , 200 μm.

Techniques Used: Chromogenic In Situ Hybridization, Expressing

BST neurons forming symmetrical synapses preferentially target GABAergic neurons in the VTA. A , B , Double-labeling immunoelectron microscopy for BDA (diffuse DAB precipitates) and TH (metal particles) in the VTA of wild-type mice. BDA-labeled terminals form symmetrical contact (arrowheads) much more frequently on TH − dendrites ( A ) than on TH + dendrites ( B ). C , D , Double-labeling immunoelectron microscopy (IEM) for BDA (diffuse DAB precipitates) and GFP (metal particles) in the VTA of GAD67–GFP knock-in mice. BDA-positive terminals form symmetrical synapses (arrowheads) much more frequently on GFP + dendrites ( C ) than on GFP − dendrites ( D ). E , F , Proportion of GABAergic (TH − or GFP + ) and DAergic (TH + or GFP − ) VTA neurons targeted by BST neurons. Scale bars, 200 nm. Dn, Dendrite; NT, nerve terminals.
Figure Legend Snippet: BST neurons forming symmetrical synapses preferentially target GABAergic neurons in the VTA. A , B , Double-labeling immunoelectron microscopy for BDA (diffuse DAB precipitates) and TH (metal particles) in the VTA of wild-type mice. BDA-labeled terminals form symmetrical contact (arrowheads) much more frequently on TH − dendrites ( A ) than on TH + dendrites ( B ). C , D , Double-labeling immunoelectron microscopy (IEM) for BDA (diffuse DAB precipitates) and GFP (metal particles) in the VTA of GAD67–GFP knock-in mice. BDA-positive terminals form symmetrical synapses (arrowheads) much more frequently on GFP + dendrites ( C ) than on GFP − dendrites ( D ). E , F , Proportion of GABAergic (TH − or GFP + ) and DAergic (TH + or GFP − ) VTA neurons targeted by BST neurons. Scale bars, 200 nm. Dn, Dendrite; NT, nerve terminals.

Techniques Used: Labeling, Immuno-Electron Microscopy, Mouse Assay, Knock-In

Postsynaptic receptor phenotype in the VTA. A , Triple immunofluorescence for GFP (green), GABA A Rα1 (red), and TH (blue) in the VTA of GAD67–GFP knock-in mice. GABA A Rα1 is expressed selectively in GFP-immunopositive GABAergic neurons (asterisk). B , C , Double-labeling preembedding immunoelectron microscopy for BDA (diffuse DAB precipitates) and GABA A Rα1 (metal particles) in the VTA of wild-type mice. At BST–VTA symmetrical synapses (enlarged in C , white arrowheads), GABA A Rα1 is localized on the synaptic and extrasynaptic membranes of dendrites (arrows). D , Postembedding immunoelectron microscopy for GABA A Rα1 (ϕ = 10 nm) and VIAAT (ϕ = 15 nm). VIAAT-labeled terminals form symmetrical synapses (white arrowheads), and GABA A Rα1 subunit is localized on the postsynaptic membrane (white arrow). E , Postembedding immunoelectron microscopy for GluA1 (ϕ = 10 nm) and VGluT2 (ϕ = 15 nm). VGluT2-labeled terminals form asymmetrical synapses (black arrowheads), and GluA1 is localized on the postsynaptic membrane (black arrows). Scale bars: A , 30 μm; B , 500 nm; C–E , 200 nm. Dn, Dendrite; NT, nerve terminals.
Figure Legend Snippet: Postsynaptic receptor phenotype in the VTA. A , Triple immunofluorescence for GFP (green), GABA A Rα1 (red), and TH (blue) in the VTA of GAD67–GFP knock-in mice. GABA A Rα1 is expressed selectively in GFP-immunopositive GABAergic neurons (asterisk). B , C , Double-labeling preembedding immunoelectron microscopy for BDA (diffuse DAB precipitates) and GABA A Rα1 (metal particles) in the VTA of wild-type mice. At BST–VTA symmetrical synapses (enlarged in C , white arrowheads), GABA A Rα1 is localized on the synaptic and extrasynaptic membranes of dendrites (arrows). D , Postembedding immunoelectron microscopy for GABA A Rα1 (ϕ = 10 nm) and VIAAT (ϕ = 15 nm). VIAAT-labeled terminals form symmetrical synapses (white arrowheads), and GABA A Rα1 subunit is localized on the postsynaptic membrane (white arrow). E , Postembedding immunoelectron microscopy for GluA1 (ϕ = 10 nm) and VGluT2 (ϕ = 15 nm). VGluT2-labeled terminals form asymmetrical synapses (black arrowheads), and GluA1 is localized on the postsynaptic membrane (black arrows). Scale bars: A , 30 μm; B , 500 nm; C–E , 200 nm. Dn, Dendrite; NT, nerve terminals.

Techniques Used: Immunofluorescence, Knock-In, Mouse Assay, Labeling, Immuno-Electron Microscopy

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    Biotechnology Information gad67
    Fluorescence in situ hybridization showing characteristic labeling patterns with the use of antisense cRNA probes for <t>GAD67</t> ( A ), VGluT1 ( B ), VGluT2 ( C ), and VGluT3 ( D ) mRNAs. Inset in D shows expression of VGluT3 mRNA in putative interneurons dispersed in the hippocampus. BLA, Basolateral amygdala; Cx, cerebral cortex; Hip, hippocampus; MHb, medial habenula; Re, reticular thalamic nucleus; Th, thalamus; VMH, ventromedial hypothalamic nucleus. Scale bars, 1 mm.
    Gad67, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 89/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gad67/product/Biotechnology Information
    Average 89 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    gad67 - by Bioz Stars, 2020-01
    89/100 stars
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    Biotechnology Information prokaryotic genome annotation pipeline
    Fluorescence in situ hybridization showing characteristic labeling patterns with the use of antisense cRNA probes for <t>GAD67</t> ( A ), VGluT1 ( B ), VGluT2 ( C ), and VGluT3 ( D ) mRNAs. Inset in D shows expression of VGluT3 mRNA in putative interneurons dispersed in the hippocampus. BLA, Basolateral amygdala; Cx, cerebral cortex; Hip, hippocampus; MHb, medial habenula; Re, reticular thalamic nucleus; Th, thalamus; VMH, ventromedial hypothalamic nucleus. Scale bars, 1 mm.
    Prokaryotic Genome Annotation Pipeline, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 91/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prokaryotic genome annotation pipeline/product/Biotechnology Information
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    Biotechnology Information ncbi
    Fluorescence in situ hybridization showing characteristic labeling patterns with the use of antisense cRNA probes for <t>GAD67</t> ( A ), VGluT1 ( B ), VGluT2 ( C ), and VGluT3 ( D ) mRNAs. Inset in D shows expression of VGluT3 mRNA in putative interneurons dispersed in the hippocampus. BLA, Basolateral amygdala; Cx, cerebral cortex; Hip, hippocampus; MHb, medial habenula; Re, reticular thalamic nucleus; Th, thalamus; VMH, ventromedial hypothalamic nucleus. Scale bars, 1 mm.
    Ncbi, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 99/100, based on 884 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biotechnology Information ncbi geo
    Fluorescence in situ hybridization showing characteristic labeling patterns with the use of antisense cRNA probes for <t>GAD67</t> ( A ), VGluT1 ( B ), VGluT2 ( C ), and VGluT3 ( D ) mRNAs. Inset in D shows expression of VGluT3 mRNA in putative interneurons dispersed in the hippocampus. BLA, Basolateral amygdala; Cx, cerebral cortex; Hip, hippocampus; MHb, medial habenula; Re, reticular thalamic nucleus; Th, thalamus; VMH, ventromedial hypothalamic nucleus. Scale bars, 1 mm.
    Ncbi Geo, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Fluorescence in situ hybridization showing characteristic labeling patterns with the use of antisense cRNA probes for GAD67 ( A ), VGluT1 ( B ), VGluT2 ( C ), and VGluT3 ( D ) mRNAs. Inset in D shows expression of VGluT3 mRNA in putative interneurons dispersed in the hippocampus. BLA, Basolateral amygdala; Cx, cerebral cortex; Hip, hippocampus; MHb, medial habenula; Re, reticular thalamic nucleus; Th, thalamus; VMH, ventromedial hypothalamic nucleus. Scale bars, 1 mm.

    Journal: The Journal of Neuroscience

    Article Title: Three Types of Neurochemical Projection from the Bed Nucleus of the Stria Terminalis to the Ventral Tegmental Area in Adult Mice

    doi: 10.1523/JNEUROSCI.4057-12.2012

    Figure Lengend Snippet: Fluorescence in situ hybridization showing characteristic labeling patterns with the use of antisense cRNA probes for GAD67 ( A ), VGluT1 ( B ), VGluT2 ( C ), and VGluT3 ( D ) mRNAs. Inset in D shows expression of VGluT3 mRNA in putative interneurons dispersed in the hippocampus. BLA, Basolateral amygdala; Cx, cerebral cortex; Hip, hippocampus; MHb, medial habenula; Re, reticular thalamic nucleus; Th, thalamus; VMH, ventromedial hypothalamic nucleus. Scale bars, 1 mm.

    Article Snippet: Digoxigenin (DIG)- or fluorescein-labeled cRNA probes were prepared to detect multiple mRNAs simultaneously. cDNA fragments of GAD67 [1036–2015; National Center for Biotechnology Information (NCBI) Reference Sequence ], mouse VGluT1 (301–1680; NCBI Reference Sequence ), mouse VGluT2 (934–2060; NCBI Reference Sequence ), and mouse VGluT3 (22–945; NCBI Reference Sequence ) were subcloned into the Bluescript II plasmid vector.

    Techniques: Fluorescence, In Situ Hybridization, Labeling, Expressing

    Chromogenic in situ hybridization showing GAD67 mRNA expression throughout the rostrocaudal extent of the BST. A , Low-power image of a coronal section through the anterior division of the BST. B–E , High-power images from the anterior ( B–D ) to the posterior ( E ) division of the BST. C is an enlarged view of A . The BST is surrounded by the caudate–putamen (CP), lateral septum (LS), preoptic area (PO), and ventral pallidum (VP). The anterior division of the BST is subdivided into the dorsal ( d ) and ventral ( v ) parts by the anterior commissure (ac). Cx, Cerebral cortex; fx, fornix; ic, internal capsule; lv, lateral ventricle; OT, olfactory tubercle; sm, stria medullaris; st, stria terminalis. Scale bars: A , 1 mm; B–E , 200 μm.

    Journal: The Journal of Neuroscience

    Article Title: Three Types of Neurochemical Projection from the Bed Nucleus of the Stria Terminalis to the Ventral Tegmental Area in Adult Mice

    doi: 10.1523/JNEUROSCI.4057-12.2012

    Figure Lengend Snippet: Chromogenic in situ hybridization showing GAD67 mRNA expression throughout the rostrocaudal extent of the BST. A , Low-power image of a coronal section through the anterior division of the BST. B–E , High-power images from the anterior ( B–D ) to the posterior ( E ) division of the BST. C is an enlarged view of A . The BST is surrounded by the caudate–putamen (CP), lateral septum (LS), preoptic area (PO), and ventral pallidum (VP). The anterior division of the BST is subdivided into the dorsal ( d ) and ventral ( v ) parts by the anterior commissure (ac). Cx, Cerebral cortex; fx, fornix; ic, internal capsule; lv, lateral ventricle; OT, olfactory tubercle; sm, stria medullaris; st, stria terminalis. Scale bars: A , 1 mm; B–E , 200 μm.

    Article Snippet: Digoxigenin (DIG)- or fluorescein-labeled cRNA probes were prepared to detect multiple mRNAs simultaneously. cDNA fragments of GAD67 [1036–2015; National Center for Biotechnology Information (NCBI) Reference Sequence ], mouse VGluT1 (301–1680; NCBI Reference Sequence ), mouse VGluT2 (934–2060; NCBI Reference Sequence ), and mouse VGluT3 (22–945; NCBI Reference Sequence ) were subcloned into the Bluescript II plasmid vector.

    Techniques: Chromogenic In Situ Hybridization, Expressing

    BST neurons forming symmetrical synapses preferentially target GABAergic neurons in the VTA. A , B , Double-labeling immunoelectron microscopy for BDA (diffuse DAB precipitates) and TH (metal particles) in the VTA of wild-type mice. BDA-labeled terminals form symmetrical contact (arrowheads) much more frequently on TH − dendrites ( A ) than on TH + dendrites ( B ). C , D , Double-labeling immunoelectron microscopy (IEM) for BDA (diffuse DAB precipitates) and GFP (metal particles) in the VTA of GAD67–GFP knock-in mice. BDA-positive terminals form symmetrical synapses (arrowheads) much more frequently on GFP + dendrites ( C ) than on GFP − dendrites ( D ). E , F , Proportion of GABAergic (TH − or GFP + ) and DAergic (TH + or GFP − ) VTA neurons targeted by BST neurons. Scale bars, 200 nm. Dn, Dendrite; NT, nerve terminals.

    Journal: The Journal of Neuroscience

    Article Title: Three Types of Neurochemical Projection from the Bed Nucleus of the Stria Terminalis to the Ventral Tegmental Area in Adult Mice

    doi: 10.1523/JNEUROSCI.4057-12.2012

    Figure Lengend Snippet: BST neurons forming symmetrical synapses preferentially target GABAergic neurons in the VTA. A , B , Double-labeling immunoelectron microscopy for BDA (diffuse DAB precipitates) and TH (metal particles) in the VTA of wild-type mice. BDA-labeled terminals form symmetrical contact (arrowheads) much more frequently on TH − dendrites ( A ) than on TH + dendrites ( B ). C , D , Double-labeling immunoelectron microscopy (IEM) for BDA (diffuse DAB precipitates) and GFP (metal particles) in the VTA of GAD67–GFP knock-in mice. BDA-positive terminals form symmetrical synapses (arrowheads) much more frequently on GFP + dendrites ( C ) than on GFP − dendrites ( D ). E , F , Proportion of GABAergic (TH − or GFP + ) and DAergic (TH + or GFP − ) VTA neurons targeted by BST neurons. Scale bars, 200 nm. Dn, Dendrite; NT, nerve terminals.

    Article Snippet: Digoxigenin (DIG)- or fluorescein-labeled cRNA probes were prepared to detect multiple mRNAs simultaneously. cDNA fragments of GAD67 [1036–2015; National Center for Biotechnology Information (NCBI) Reference Sequence ], mouse VGluT1 (301–1680; NCBI Reference Sequence ), mouse VGluT2 (934–2060; NCBI Reference Sequence ), and mouse VGluT3 (22–945; NCBI Reference Sequence ) were subcloned into the Bluescript II plasmid vector.

    Techniques: Labeling, Immuno-Electron Microscopy, Mouse Assay, Knock-In

    Postsynaptic receptor phenotype in the VTA. A , Triple immunofluorescence for GFP (green), GABA A Rα1 (red), and TH (blue) in the VTA of GAD67–GFP knock-in mice. GABA A Rα1 is expressed selectively in GFP-immunopositive GABAergic neurons (asterisk). B , C , Double-labeling preembedding immunoelectron microscopy for BDA (diffuse DAB precipitates) and GABA A Rα1 (metal particles) in the VTA of wild-type mice. At BST–VTA symmetrical synapses (enlarged in C , white arrowheads), GABA A Rα1 is localized on the synaptic and extrasynaptic membranes of dendrites (arrows). D , Postembedding immunoelectron microscopy for GABA A Rα1 (ϕ = 10 nm) and VIAAT (ϕ = 15 nm). VIAAT-labeled terminals form symmetrical synapses (white arrowheads), and GABA A Rα1 subunit is localized on the postsynaptic membrane (white arrow). E , Postembedding immunoelectron microscopy for GluA1 (ϕ = 10 nm) and VGluT2 (ϕ = 15 nm). VGluT2-labeled terminals form asymmetrical synapses (black arrowheads), and GluA1 is localized on the postsynaptic membrane (black arrows). Scale bars: A , 30 μm; B , 500 nm; C–E , 200 nm. Dn, Dendrite; NT, nerve terminals.

    Journal: The Journal of Neuroscience

    Article Title: Three Types of Neurochemical Projection from the Bed Nucleus of the Stria Terminalis to the Ventral Tegmental Area in Adult Mice

    doi: 10.1523/JNEUROSCI.4057-12.2012

    Figure Lengend Snippet: Postsynaptic receptor phenotype in the VTA. A , Triple immunofluorescence for GFP (green), GABA A Rα1 (red), and TH (blue) in the VTA of GAD67–GFP knock-in mice. GABA A Rα1 is expressed selectively in GFP-immunopositive GABAergic neurons (asterisk). B , C , Double-labeling preembedding immunoelectron microscopy for BDA (diffuse DAB precipitates) and GABA A Rα1 (metal particles) in the VTA of wild-type mice. At BST–VTA symmetrical synapses (enlarged in C , white arrowheads), GABA A Rα1 is localized on the synaptic and extrasynaptic membranes of dendrites (arrows). D , Postembedding immunoelectron microscopy for GABA A Rα1 (ϕ = 10 nm) and VIAAT (ϕ = 15 nm). VIAAT-labeled terminals form symmetrical synapses (white arrowheads), and GABA A Rα1 subunit is localized on the postsynaptic membrane (white arrow). E , Postembedding immunoelectron microscopy for GluA1 (ϕ = 10 nm) and VGluT2 (ϕ = 15 nm). VGluT2-labeled terminals form asymmetrical synapses (black arrowheads), and GluA1 is localized on the postsynaptic membrane (black arrows). Scale bars: A , 30 μm; B , 500 nm; C–E , 200 nm. Dn, Dendrite; NT, nerve terminals.

    Article Snippet: Digoxigenin (DIG)- or fluorescein-labeled cRNA probes were prepared to detect multiple mRNAs simultaneously. cDNA fragments of GAD67 [1036–2015; National Center for Biotechnology Information (NCBI) Reference Sequence ], mouse VGluT1 (301–1680; NCBI Reference Sequence ), mouse VGluT2 (934–2060; NCBI Reference Sequence ), and mouse VGluT3 (22–945; NCBI Reference Sequence ) were subcloned into the Bluescript II plasmid vector.

    Techniques: Immunofluorescence, Knock-In, Mouse Assay, Labeling, Immuno-Electron Microscopy