biosprint96 one for all vet kit  (Qiagen)

 
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    Name:
    BioSprint 96 One For All Vet Kit
    Description:
    For rapid and economical automated purification of viral nucleic acids and bacterial DNA Kit contents For 384 preps Large 96 Rod Covers 96 Well Microplates MP S Blocks MagAttract Suspension G buffers and reagents Benefits Simultaneous purification of viral DNA and RNA and bacterial DNA Pure nucleic acids ready for use in downstream applications Kit contains everything you need from reagents to plasticware Fast processing of up to 96 samples per run Automated procedure saves time and eff
    Catalog Number:
    947057
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    BioSprint 96 One For All Vet Kit
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    Structured Review

    Qiagen biosprint96 one for all vet kit
    BioSprint 96 One For All Vet Kit
    For rapid and economical automated purification of viral nucleic acids and bacterial DNA Kit contents For 384 preps Large 96 Rod Covers 96 Well Microplates MP S Blocks MagAttract Suspension G buffers and reagents Benefits Simultaneous purification of viral DNA and RNA and bacterial DNA Pure nucleic acids ready for use in downstream applications Kit contains everything you need from reagents to plasticware Fast processing of up to 96 samples per run Automated procedure saves time and eff
    https://www.bioz.com/result/biosprint96 one for all vet kit/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biosprint96 one for all vet kit - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Diagnostic Assay:

    Article Title: Evaluation of Two Magnetic-Bead-Based Viral Nucleic Acid Purification Kits and Three Real-Time Reverse Transcription-PCR Reagent Systems in Two TaqMan Assays for Equine Arteritis Virus Detection in Semen ▿Evaluation of Two Magnetic-Bead-Based Viral Nucleic Acid Purification Kits and Three Real-Time Reverse Transcription-PCR Reagent Systems in Two TaqMan Assays for Equine Arteritis Virus Detectio
    Article Snippet: .. The objective of the current study was to see if we could increase the diagnostic sensitivity of two previously described one-tube rRT-PCR assays (T1 and T2) ( , ) using one-step rRT-PCR reagents from three different commercial kits (QuantiFast Probe RT-PCR + ROX viral kit [Qiagen, Valencia, CA], TaqMan one-step RT-PCR master mix reagent kit, and TaqMan EZ RT-PCR core reagent kit [both from Applied Biosystems, Foster City, CA]) in combination with RNA extraction with magnetic-bead-based nucleic acid extraction kits (MagMAX-96 viral RNA isolation kit [Applied Biosystems, Foster City, CA] and BioSprint 96 One-For-All Vet kit [Qiagen, Valencia, CA]). .. In order to compare the two assays in a standard and normalized manner, the commercial kits and rRT-PCR cycle parameters used in this study followed the manufacturers ' recommendations (see Table S1 in the supplemental material).

    DNA Extraction:

    Article Title: A Rapid Method for Quantifying Viable Mycobacterium avium subsp. paratuberculosis in Cellular Infection Assays
    Article Snippet: .. Briefly, DNA isolation from each sample was done by using a combined method involving mechanical cell disruption followed by magnetic bead-based DNA purification using the BioSprint 96 One-for-All Vet kit (Qiagen). .. All M. avium subsp. paratuberculosis solutions were added to 2-ml conical-base screw-cap tubes containing 200 μl of RLT buffer and 0.3 g of zirconia-silica beads (BioSpec Products, Inc., Daintree Scientific).

    RNA Extraction:

    Article Title: Evaluation of Two Magnetic-Bead-Based Viral Nucleic Acid Purification Kits and Three Real-Time Reverse Transcription-PCR Reagent Systems in Two TaqMan Assays for Equine Arteritis Virus Detection in Semen ▿Evaluation of Two Magnetic-Bead-Based Viral Nucleic Acid Purification Kits and Three Real-Time Reverse Transcription-PCR Reagent Systems in Two TaqMan Assays for Equine Arteritis Virus Detectio
    Article Snippet: .. The objective of the current study was to see if we could increase the diagnostic sensitivity of two previously described one-tube rRT-PCR assays (T1 and T2) ( , ) using one-step rRT-PCR reagents from three different commercial kits (QuantiFast Probe RT-PCR + ROX viral kit [Qiagen, Valencia, CA], TaqMan one-step RT-PCR master mix reagent kit, and TaqMan EZ RT-PCR core reagent kit [both from Applied Biosystems, Foster City, CA]) in combination with RNA extraction with magnetic-bead-based nucleic acid extraction kits (MagMAX-96 viral RNA isolation kit [Applied Biosystems, Foster City, CA] and BioSprint 96 One-For-All Vet kit [Qiagen, Valencia, CA]). .. In order to compare the two assays in a standard and normalized manner, the commercial kits and rRT-PCR cycle parameters used in this study followed the manufacturers ' recommendations (see Table S1 in the supplemental material).

    Isolation:

    Article Title: Evaluation of Two Magnetic-Bead-Based Viral Nucleic Acid Purification Kits and Three Real-Time Reverse Transcription-PCR Reagent Systems in Two TaqMan Assays for Equine Arteritis Virus Detection in Semen ▿Evaluation of Two Magnetic-Bead-Based Viral Nucleic Acid Purification Kits and Three Real-Time Reverse Transcription-PCR Reagent Systems in Two TaqMan Assays for Equine Arteritis Virus Detectio
    Article Snippet: .. The objective of the current study was to see if we could increase the diagnostic sensitivity of two previously described one-tube rRT-PCR assays (T1 and T2) ( , ) using one-step rRT-PCR reagents from three different commercial kits (QuantiFast Probe RT-PCR + ROX viral kit [Qiagen, Valencia, CA], TaqMan one-step RT-PCR master mix reagent kit, and TaqMan EZ RT-PCR core reagent kit [both from Applied Biosystems, Foster City, CA]) in combination with RNA extraction with magnetic-bead-based nucleic acid extraction kits (MagMAX-96 viral RNA isolation kit [Applied Biosystems, Foster City, CA] and BioSprint 96 One-For-All Vet kit [Qiagen, Valencia, CA]). .. In order to compare the two assays in a standard and normalized manner, the commercial kits and rRT-PCR cycle parameters used in this study followed the manufacturers ' recommendations (see Table S1 in the supplemental material).

    Quantitative RT-PCR:

    Article Title: Evaluation of Two Magnetic-Bead-Based Viral Nucleic Acid Purification Kits and Three Real-Time Reverse Transcription-PCR Reagent Systems in Two TaqMan Assays for Equine Arteritis Virus Detection in Semen ▿Evaluation of Two Magnetic-Bead-Based Viral Nucleic Acid Purification Kits and Three Real-Time Reverse Transcription-PCR Reagent Systems in Two TaqMan Assays for Equine Arteritis Virus Detectio
    Article Snippet: .. The objective of the current study was to see if we could increase the diagnostic sensitivity of two previously described one-tube rRT-PCR assays (T1 and T2) ( , ) using one-step rRT-PCR reagents from three different commercial kits (QuantiFast Probe RT-PCR + ROX viral kit [Qiagen, Valencia, CA], TaqMan one-step RT-PCR master mix reagent kit, and TaqMan EZ RT-PCR core reagent kit [both from Applied Biosystems, Foster City, CA]) in combination with RNA extraction with magnetic-bead-based nucleic acid extraction kits (MagMAX-96 viral RNA isolation kit [Applied Biosystems, Foster City, CA] and BioSprint 96 One-For-All Vet kit [Qiagen, Valencia, CA]). .. In order to compare the two assays in a standard and normalized manner, the commercial kits and rRT-PCR cycle parameters used in this study followed the manufacturers ' recommendations (see Table S1 in the supplemental material).

    Purification:

    Article Title: Outbreak of porcine epidemic diarrhoea virus ( PEDV) in Abruzzi region, central‐Italy
    Article Snippet: .. A faecal suspension was prepared for each sample using 1 g of faeces in a total volume of 10 mL of phosphate‐buffered saline solution with antibiotics; 100 μ L of the supernatants was used for RNA purification using BioSprint 96 One‐For‐All Vet Kit (Qiagen), following manufacturer's instructions. .. RNAs were tested by real‐time RT‐PCR (RT‐qPCR) using a commercial kit, ViroReal® Kit PEDV & TGEV (Ingenetix).

    Article Title: Culture-Independent Identification of Mycobacterium avium Subspecies paratuberculosis in Ovine Tissues: Comparison with Bacterial Culture and Histopathological Lesions
    Article Snippet: .. DNA Purification DNA was purified using the Biosprint® 96 One-for-all Vet kit (Qiagen). .. Briefly, 400 µL of the supernatant obtained above was transferred to a 96-well lysate plate containing 40 µL proteinase K (Qiagen) to which 300 µL of magnetic bead suspension comprising 25 µL MagAttract suspension G (Qiagen) in 300 µL isopropanol (Sigma-Aldrich) was added.

    Cell Culture:

    Article Title: Hypsugopoxvirus: A Novel Poxvirus Isolated from Hypsugo savii in Italy
    Article Snippet: .. Molecular Analysis Viral DNA was extracted from 200 μL of positive cell culture supernatants using a BioSprint 96 One-For-All Vet Kit (Qiagen S.p.A., Milan, Italy). .. Sequencing libraries were made with a Nextera Flex kit (Illumina Inc. San Diego, CA, USA) in accordance with the manufacturer’s instructions.

    DNA Purification:

    Article Title: A Rapid Method for Quantifying Viable Mycobacterium avium subsp. paratuberculosis in Cellular Infection Assays
    Article Snippet: .. Briefly, DNA isolation from each sample was done by using a combined method involving mechanical cell disruption followed by magnetic bead-based DNA purification using the BioSprint 96 One-for-All Vet kit (Qiagen). .. All M. avium subsp. paratuberculosis solutions were added to 2-ml conical-base screw-cap tubes containing 200 μl of RLT buffer and 0.3 g of zirconia-silica beads (BioSpec Products, Inc., Daintree Scientific).

    Article Title: Determining an optimal pool size for testing beef herds for Johne’s disease in Australia
    Article Snippet: .. The DNA purification was conducted with the BioSprint 96 One-for-all Vet kit (Qiagen) using 96-well plates and an automated magnetic particle processor (MagMax Express 96; Life Technologies). .. A process control (buffers alone) and Mptb positive control (5.67 x 103 Mptb /well) were included during the DNA purification step.

    Article Title: Culture-Independent Identification of Mycobacterium avium Subspecies paratuberculosis in Ovine Tissues: Comparison with Bacterial Culture and Histopathological Lesions
    Article Snippet: .. DNA Purification DNA was purified using the Biosprint® 96 One-for-all Vet kit (Qiagen). .. Briefly, 400 µL of the supernatant obtained above was transferred to a 96-well lysate plate containing 40 µL proteinase K (Qiagen) to which 300 µL of magnetic bead suspension comprising 25 µL MagAttract suspension G (Qiagen) in 300 µL isopropanol (Sigma-Aldrich) was added.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Evaluation of Two Magnetic-Bead-Based Viral Nucleic Acid Purification Kits and Three Real-Time Reverse Transcription-PCR Reagent Systems in Two TaqMan Assays for Equine Arteritis Virus Detection in Semen ▿Evaluation of Two Magnetic-Bead-Based Viral Nucleic Acid Purification Kits and Three Real-Time Reverse Transcription-PCR Reagent Systems in Two TaqMan Assays for Equine Arteritis Virus Detectio
    Article Snippet: .. The objective of the current study was to see if we could increase the diagnostic sensitivity of two previously described one-tube rRT-PCR assays (T1 and T2) ( , ) using one-step rRT-PCR reagents from three different commercial kits (QuantiFast Probe RT-PCR + ROX viral kit [Qiagen, Valencia, CA], TaqMan one-step RT-PCR master mix reagent kit, and TaqMan EZ RT-PCR core reagent kit [both from Applied Biosystems, Foster City, CA]) in combination with RNA extraction with magnetic-bead-based nucleic acid extraction kits (MagMAX-96 viral RNA isolation kit [Applied Biosystems, Foster City, CA] and BioSprint 96 One-For-All Vet kit [Qiagen, Valencia, CA]). .. In order to compare the two assays in a standard and normalized manner, the commercial kits and rRT-PCR cycle parameters used in this study followed the manufacturers ' recommendations (see Table S1 in the supplemental material).

    Lysis:

    Article Title: Variation in susceptibility of different breeds of sheep to Mycobacterium avium subspecies paratuberculosis following experimental inoculation
    Article Snippet: .. After vigorous shaking, this was allowed to settle for 30 min and 3–5 mL of supernatant was centrifuged at 1231 × g for 30 min. To the pellet, 600 μL Lysis/binding solution (597.2 μL Buffer RLT and 2.8 μL Carrier RNA; Biosprint® 96 One-For-All Vet kit, Qiagen) was added, then transferred to a 2 mL screw capped tube containing 0.3 g of Zirconia/Silica beads (BioSpec Products Inc, Daintree Scientific) and disrupted using a mechanical cell disruptor/bead beater. .. The supernantant (400 μL) was transferred to a deep 96 well plate, with 40 μL Proteinase K and 300 μL Magnetic Bead mix (Biosprint® 96 One-For-All Vet kit, Qiagen).

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  • 99
    Qiagen biosprint96 one for all vet kit
    Biosprint96 One For All Vet Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biosprint96 one for all vet kit/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biosprint96 one for all vet kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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