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Bio-Rad biosilect sec400 gel filtration column
Gel filtration of KI extract by <t>BioSilect</t> <t>SEC400</t> column. (A) KI extract from KCl-treated axonemes was loaded on a gel filtration column (300 × 7.8 mm) and separated at a flow rate of 1.0 ml/min. Fractions (200 μl) were collected. The numbers above the chromatogram indicate the fraction number. Arrows show the elution positions of molecular mass markers as follows: thyroglobulin (670 kDa), immunoglobulin G (150 kDa), ovalbumin (44 kDa), and myoglobin (17 kDa). (B) Proteins in the fraction 4–25 were separated by 10% SDS-PAGE and immunoblotted by anti-LRR37 and anti-RSP3 antibodies. Both proteins were coeluted at around the position with molecular mass of 1300 kDa.
Biosilect Sec400 Gel Filtration Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biosilect sec400 gel filtration column/product/Bio-Rad
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
biosilect sec400 gel filtration column - by Bioz Stars, 2020-09
85/100 stars

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1) Product Images from "Identification of a Novel Leucine-rich Repeat Protein as a Component of Flagellar Radial Spoke in the Ascidian Ciona intestinalis"

Article Title: Identification of a Novel Leucine-rich Repeat Protein as a Component of Flagellar Radial Spoke in the Ascidian Ciona intestinalis

Journal: Molecular Biology of the Cell

doi: 10.1091/mbc.02-06-0089

Gel filtration of KI extract by BioSilect SEC400 column. (A) KI extract from KCl-treated axonemes was loaded on a gel filtration column (300 × 7.8 mm) and separated at a flow rate of 1.0 ml/min. Fractions (200 μl) were collected. The numbers above the chromatogram indicate the fraction number. Arrows show the elution positions of molecular mass markers as follows: thyroglobulin (670 kDa), immunoglobulin G (150 kDa), ovalbumin (44 kDa), and myoglobin (17 kDa). (B) Proteins in the fraction 4–25 were separated by 10% SDS-PAGE and immunoblotted by anti-LRR37 and anti-RSP3 antibodies. Both proteins were coeluted at around the position with molecular mass of 1300 kDa.
Figure Legend Snippet: Gel filtration of KI extract by BioSilect SEC400 column. (A) KI extract from KCl-treated axonemes was loaded on a gel filtration column (300 × 7.8 mm) and separated at a flow rate of 1.0 ml/min. Fractions (200 μl) were collected. The numbers above the chromatogram indicate the fraction number. Arrows show the elution positions of molecular mass markers as follows: thyroglobulin (670 kDa), immunoglobulin G (150 kDa), ovalbumin (44 kDa), and myoglobin (17 kDa). (B) Proteins in the fraction 4–25 were separated by 10% SDS-PAGE and immunoblotted by anti-LRR37 and anti-RSP3 antibodies. Both proteins were coeluted at around the position with molecular mass of 1300 kDa.

Techniques Used: Filtration, Flow Cytometry, SDS Page

Related Articles

Flow Cytometry:

Article Title: Identification of a Novel Leucine-rich Repeat Protein as a Component of Flagellar Radial Spoke in the Ascidian Ciona intestinalis
Article Snippet: .. The supernatant was separated on a BioSilect SEC400 gel filtration column (300 × 7.8 mm; Bio-Rad , Hercules, CA) at a flow rate of 1.0 ml/min. .. Fractions (200 μl) were collected and proteins in each fraction were separated by SDS-PAGE with 10% polyacrylamide as the separating gel.

Filtration:

Article Title: Identification of a Novel Leucine-rich Repeat Protein as a Component of Flagellar Radial Spoke in the Ascidian Ciona intestinalis
Article Snippet: .. The supernatant was separated on a BioSilect SEC400 gel filtration column (300 × 7.8 mm; Bio-Rad , Hercules, CA) at a flow rate of 1.0 ml/min. .. Fractions (200 μl) were collected and proteins in each fraction were separated by SDS-PAGE with 10% polyacrylamide as the separating gel.

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    Bio-Rad biosilect sec400 gel filtration column
    Gel filtration of KI extract by <t>BioSilect</t> <t>SEC400</t> column. (A) KI extract from KCl-treated axonemes was loaded on a gel filtration column (300 × 7.8 mm) and separated at a flow rate of 1.0 ml/min. Fractions (200 μl) were collected. The numbers above the chromatogram indicate the fraction number. Arrows show the elution positions of molecular mass markers as follows: thyroglobulin (670 kDa), immunoglobulin G (150 kDa), ovalbumin (44 kDa), and myoglobin (17 kDa). (B) Proteins in the fraction 4–25 were separated by 10% SDS-PAGE and immunoblotted by anti-LRR37 and anti-RSP3 antibodies. Both proteins were coeluted at around the position with molecular mass of 1300 kDa.
    Biosilect Sec400 Gel Filtration Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biosilect sec400 gel filtration column/product/Bio-Rad
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biosilect sec400 gel filtration column - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

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    Gel filtration of KI extract by BioSilect SEC400 column. (A) KI extract from KCl-treated axonemes was loaded on a gel filtration column (300 × 7.8 mm) and separated at a flow rate of 1.0 ml/min. Fractions (200 μl) were collected. The numbers above the chromatogram indicate the fraction number. Arrows show the elution positions of molecular mass markers as follows: thyroglobulin (670 kDa), immunoglobulin G (150 kDa), ovalbumin (44 kDa), and myoglobin (17 kDa). (B) Proteins in the fraction 4–25 were separated by 10% SDS-PAGE and immunoblotted by anti-LRR37 and anti-RSP3 antibodies. Both proteins were coeluted at around the position with molecular mass of 1300 kDa.

    Journal: Molecular Biology of the Cell

    Article Title: Identification of a Novel Leucine-rich Repeat Protein as a Component of Flagellar Radial Spoke in the Ascidian Ciona intestinalis

    doi: 10.1091/mbc.02-06-0089

    Figure Lengend Snippet: Gel filtration of KI extract by BioSilect SEC400 column. (A) KI extract from KCl-treated axonemes was loaded on a gel filtration column (300 × 7.8 mm) and separated at a flow rate of 1.0 ml/min. Fractions (200 μl) were collected. The numbers above the chromatogram indicate the fraction number. Arrows show the elution positions of molecular mass markers as follows: thyroglobulin (670 kDa), immunoglobulin G (150 kDa), ovalbumin (44 kDa), and myoglobin (17 kDa). (B) Proteins in the fraction 4–25 were separated by 10% SDS-PAGE and immunoblotted by anti-LRR37 and anti-RSP3 antibodies. Both proteins were coeluted at around the position with molecular mass of 1300 kDa.

    Article Snippet: The supernatant was separated on a BioSilect SEC400 gel filtration column (300 × 7.8 mm; Bio-Rad , Hercules, CA) at a flow rate of 1.0 ml/min.

    Techniques: Filtration, Flow Cytometry, SDS Page