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Flexcell International Corp bioflex 6 well plates
Bioflex 6 Well Plates, supplied by Flexcell International Corp, used in various techniques. Bioz Stars score: 89/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bioflex 6 well plates/product/Flexcell International Corp
Average 89 stars, based on 11 article reviews
Price from $9.99 to $1999.99
bioflex 6 well plates - by Bioz Stars, 2020-09
89/100 stars

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Article Title: Thermosensitive and Highly Flexible Hydrogels Capable of Stimulating Cardiac Differentiation of Cardiosphere-Derived Cells under Static and Dynamic Mechanical Training Conditions
Article Snippet: BioFlex 6-well plates were purchased from Flexcell International Corporation.

Article Title: α1β1 Integrin/Rac1-Dependent Mesangial Invasion of Glomerular Capillaries in Alport Syndrome
Article Snippet: Low-passage, subconfluent, primary mesangial cells were trypsinized and seeded onto Bioflex 6-well plates (Flexcell International Corp., Hillsborough, NC) coated with rat tail type I collagen (BD Biosciences).

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    Flexcell International Corp bioflex 6 well plate
    Mechanical stimulation and inducing medium (IM) synergistically promote keratocyte differentiation. PDLSCs were seeded on Bioflex 6-well plates, with treatment of IM, static mechanics (SM) or a combination of both (IM+SM) for 6 days. (A) Gene expression was evaluated by quantitative PCR. Levels at day 0 (unstimulated PDLSCs) were set as 1. The expression was compared between IM and SM, IM and IM+SM, SM and IM+SM, IM+SM and primary in vitro cultured keratocytes at passage 2.  * Significant difference at P
    Bioflex 6 Well Plate, supplied by Flexcell International Corp, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bioflex 6 well plate/product/Flexcell International Corp
    Average 93 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    bioflex 6 well plate - by Bioz Stars, 2020-09
    93/100 stars
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    Mechanical stimulation and inducing medium (IM) synergistically promote keratocyte differentiation. PDLSCs were seeded on Bioflex 6-well plates, with treatment of IM, static mechanics (SM) or a combination of both (IM+SM) for 6 days. (A) Gene expression was evaluated by quantitative PCR. Levels at day 0 (unstimulated PDLSCs) were set as 1. The expression was compared between IM and SM, IM and IM+SM, SM and IM+SM, IM+SM and primary in vitro cultured keratocytes at passage 2.  * Significant difference at P

    Journal: The British Journal of Ophthalmology

    Article Title: Mechanical stress potentiates the differentiation of periodontal ligament stem cells into keratocytes

    doi: 10.1136/bjophthalmol-2017-311150

    Figure Lengend Snippet: Mechanical stimulation and inducing medium (IM) synergistically promote keratocyte differentiation. PDLSCs were seeded on Bioflex 6-well plates, with treatment of IM, static mechanics (SM) or a combination of both (IM+SM) for 6 days. (A) Gene expression was evaluated by quantitative PCR. Levels at day 0 (unstimulated PDLSCs) were set as 1. The expression was compared between IM and SM, IM and IM+SM, SM and IM+SM, IM+SM and primary in vitro cultured keratocytes at passage 2. * Significant difference at P

    Article Snippet: PDLSCs were seeded on Collagen I bonded Bioflex 6-well plate (Flexcell International Corporation, Burlington, North Carolina, USA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, In Vitro, Cell Culture

    Typical markers of corneal stroma are expressed in the cell sheets constructed by PDLSCs exposed to mechanical stimulation and inducing medium. PDLSCs were seeded on Bioflex 6-well plates, with treatment of static mechanical strain and inducing medium for 12 days to form a cell sheet. The expression of typical corneal stroma markers was evaluated by immunofluorescence staining. The right column is the merged picture of left column (corneal stroma markers staining) and middle column (DAPI staining). ‘NTC’ indicates negative control without primary antibody. PDLSCs, periodontal ligament stem cells.

    Journal: The British Journal of Ophthalmology

    Article Title: Mechanical stress potentiates the differentiation of periodontal ligament stem cells into keratocytes

    doi: 10.1136/bjophthalmol-2017-311150

    Figure Lengend Snippet: Typical markers of corneal stroma are expressed in the cell sheets constructed by PDLSCs exposed to mechanical stimulation and inducing medium. PDLSCs were seeded on Bioflex 6-well plates, with treatment of static mechanical strain and inducing medium for 12 days to form a cell sheet. The expression of typical corneal stroma markers was evaluated by immunofluorescence staining. The right column is the merged picture of left column (corneal stroma markers staining) and middle column (DAPI staining). ‘NTC’ indicates negative control without primary antibody. PDLSCs, periodontal ligament stem cells.

    Article Snippet: PDLSCs were seeded on Collagen I bonded Bioflex 6-well plate (Flexcell International Corporation, Burlington, North Carolina, USA).

    Techniques: Construct, Expressing, Immunofluorescence, Staining, Negative Control

    Mechanical stimulation and inducing medium (IM) exert superior effect on keratocyte differentiation when they are working together at the same time. PDLSCs were seeded on Bioflex 6-well plates, with treatment of IM and static mechanics (SM) together for 6 days (IM+SM), or IM for the first 3 days and SM for the following 3 days (IM-SM), or SM for the first 3 days and IM for the following 3 days (SM-IM). (A) Gene expression was evaluated by quantitative PCR. Levels of IM+SM were set as 1.  * Significant difference at P

    Journal: The British Journal of Ophthalmology

    Article Title: Mechanical stress potentiates the differentiation of periodontal ligament stem cells into keratocytes

    doi: 10.1136/bjophthalmol-2017-311150

    Figure Lengend Snippet: Mechanical stimulation and inducing medium (IM) exert superior effect on keratocyte differentiation when they are working together at the same time. PDLSCs were seeded on Bioflex 6-well plates, with treatment of IM and static mechanics (SM) together for 6 days (IM+SM), or IM for the first 3 days and SM for the following 3 days (IM-SM), or SM for the first 3 days and IM for the following 3 days (SM-IM). (A) Gene expression was evaluated by quantitative PCR. Levels of IM+SM were set as 1. * Significant difference at P

    Article Snippet: PDLSCs were seeded on Collagen I bonded Bioflex 6-well plate (Flexcell International Corporation, Burlington, North Carolina, USA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    Mechanical stimulation and inducing medium generate a transparent and multilamellar cell sheet. PDLSCs were seeded on collagen I bonded Bioflex 6-well plates and exposed to static mechanical strain and inducing medium for 12 days to form a cell sheet. The collagen membrane of the Bioflex 6-well plate itself (A) and the collagen membrane with the formed cell sheet (B) were both transparent. (C) The light transmission of the cell sheet was measured in the wavelength from 400 nm to 850 nm. (D) The formed cell sheet was found to be a multilamellar structure, as revealed by DAPI staining; white arrows indicating nuclei that are focused on one layer, and red arrows indicating nuclei that were not focused and thus on another layer. PDLSCs, periodontal ligament stem cells.

    Journal: The British Journal of Ophthalmology

    Article Title: Mechanical stress potentiates the differentiation of periodontal ligament stem cells into keratocytes

    doi: 10.1136/bjophthalmol-2017-311150

    Figure Lengend Snippet: Mechanical stimulation and inducing medium generate a transparent and multilamellar cell sheet. PDLSCs were seeded on collagen I bonded Bioflex 6-well plates and exposed to static mechanical strain and inducing medium for 12 days to form a cell sheet. The collagen membrane of the Bioflex 6-well plate itself (A) and the collagen membrane with the formed cell sheet (B) were both transparent. (C) The light transmission of the cell sheet was measured in the wavelength from 400 nm to 850 nm. (D) The formed cell sheet was found to be a multilamellar structure, as revealed by DAPI staining; white arrows indicating nuclei that are focused on one layer, and red arrows indicating nuclei that were not focused and thus on another layer. PDLSCs, periodontal ligament stem cells.

    Article Snippet: PDLSCs were seeded on Collagen I bonded Bioflex 6-well plate (Flexcell International Corporation, Burlington, North Carolina, USA).

    Techniques: Transmission Assay, Staining

    Mechanical stimulation and inducing medium (IM) synergistically promote keratocyte differentiation. PDLSCs were seeded on Bioflex 6-well plates, with treatment of IM, static mechanics (SM) or a combination of both (IM+SM) for 6 days. (A) Gene expression was evaluated by quantitative PCR. Levels at day 0 (unstimulated PDLSCs) were set as 1. The expression was compared between IM and SM, IM and IM+SM, SM and IM+SM, IM+SM and primary in vitro cultured keratocytes at passage 2.  * Significant difference at P

    Journal: The British Journal of Ophthalmology

    Article Title: Mechanical stress potentiates the differentiation of periodontal ligament stem cells into keratocytes

    doi: 10.1136/bjophthalmol-2017-311150

    Figure Lengend Snippet: Mechanical stimulation and inducing medium (IM) synergistically promote keratocyte differentiation. PDLSCs were seeded on Bioflex 6-well plates, with treatment of IM, static mechanics (SM) or a combination of both (IM+SM) for 6 days. (A) Gene expression was evaluated by quantitative PCR. Levels at day 0 (unstimulated PDLSCs) were set as 1. The expression was compared between IM and SM, IM and IM+SM, SM and IM+SM, IM+SM and primary in vitro cultured keratocytes at passage 2. * Significant difference at P

    Article Snippet: PDLSCs (2×105 /well) were seeded on Bioflex 6-well plate and cultured in regular growing medium for 1 day before treatments.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, In Vitro, Cell Culture

    Typical markers of corneal stroma are expressed in the cell sheets constructed by PDLSCs exposed to mechanical stimulation and inducing medium. PDLSCs were seeded on Bioflex 6-well plates, with treatment of static mechanical strain and inducing medium for 12 days to form a cell sheet. The expression of typical corneal stroma markers was evaluated by immunofluorescence staining. The right column is the merged picture of left column (corneal stroma markers staining) and middle column (DAPI staining). ‘NTC’ indicates negative control without primary antibody. PDLSCs, periodontal ligament stem cells.

    Journal: The British Journal of Ophthalmology

    Article Title: Mechanical stress potentiates the differentiation of periodontal ligament stem cells into keratocytes

    doi: 10.1136/bjophthalmol-2017-311150

    Figure Lengend Snippet: Typical markers of corneal stroma are expressed in the cell sheets constructed by PDLSCs exposed to mechanical stimulation and inducing medium. PDLSCs were seeded on Bioflex 6-well plates, with treatment of static mechanical strain and inducing medium for 12 days to form a cell sheet. The expression of typical corneal stroma markers was evaluated by immunofluorescence staining. The right column is the merged picture of left column (corneal stroma markers staining) and middle column (DAPI staining). ‘NTC’ indicates negative control without primary antibody. PDLSCs, periodontal ligament stem cells.

    Article Snippet: PDLSCs (2×105 /well) were seeded on Bioflex 6-well plate and cultured in regular growing medium for 1 day before treatments.

    Techniques: Construct, Expressing, Immunofluorescence, Staining, Negative Control

    Mechanical stimulation and inducing medium (IM) exert superior effect on keratocyte differentiation when they are working together at the same time. PDLSCs were seeded on Bioflex 6-well plates, with treatment of IM and static mechanics (SM) together for 6 days (IM+SM), or IM for the first 3 days and SM for the following 3 days (IM-SM), or SM for the first 3 days and IM for the following 3 days (SM-IM). (A) Gene expression was evaluated by quantitative PCR. Levels of IM+SM were set as 1.  * Significant difference at P

    Journal: The British Journal of Ophthalmology

    Article Title: Mechanical stress potentiates the differentiation of periodontal ligament stem cells into keratocytes

    doi: 10.1136/bjophthalmol-2017-311150

    Figure Lengend Snippet: Mechanical stimulation and inducing medium (IM) exert superior effect on keratocyte differentiation when they are working together at the same time. PDLSCs were seeded on Bioflex 6-well plates, with treatment of IM and static mechanics (SM) together for 6 days (IM+SM), or IM for the first 3 days and SM for the following 3 days (IM-SM), or SM for the first 3 days and IM for the following 3 days (SM-IM). (A) Gene expression was evaluated by quantitative PCR. Levels of IM+SM were set as 1. * Significant difference at P

    Article Snippet: PDLSCs (2×105 /well) were seeded on Bioflex 6-well plate and cultured in regular growing medium for 1 day before treatments.

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    Mechanical stimulation and inducing medium generate a transparent and multilamellar cell sheet. PDLSCs were seeded on collagen I bonded Bioflex 6-well plates and exposed to static mechanical strain and inducing medium for 12 days to form a cell sheet. The collagen membrane of the Bioflex 6-well plate itself (A) and the collagen membrane with the formed cell sheet (B) were both transparent. (C) The light transmission of the cell sheet was measured in the wavelength from 400 nm to 850 nm. (D) The formed cell sheet was found to be a multilamellar structure, as revealed by DAPI staining; white arrows indicating nuclei that are focused on one layer, and red arrows indicating nuclei that were not focused and thus on another layer. PDLSCs, periodontal ligament stem cells.

    Journal: The British Journal of Ophthalmology

    Article Title: Mechanical stress potentiates the differentiation of periodontal ligament stem cells into keratocytes

    doi: 10.1136/bjophthalmol-2017-311150

    Figure Lengend Snippet: Mechanical stimulation and inducing medium generate a transparent and multilamellar cell sheet. PDLSCs were seeded on collagen I bonded Bioflex 6-well plates and exposed to static mechanical strain and inducing medium for 12 days to form a cell sheet. The collagen membrane of the Bioflex 6-well plate itself (A) and the collagen membrane with the formed cell sheet (B) were both transparent. (C) The light transmission of the cell sheet was measured in the wavelength from 400 nm to 850 nm. (D) The formed cell sheet was found to be a multilamellar structure, as revealed by DAPI staining; white arrows indicating nuclei that are focused on one layer, and red arrows indicating nuclei that were not focused and thus on another layer. PDLSCs, periodontal ligament stem cells.

    Article Snippet: PDLSCs (2×105 /well) were seeded on Bioflex 6-well plate and cultured in regular growing medium for 1 day before treatments.

    Techniques: Transmission Assay, Staining

    Mechanical strain and hypoxia activates S6K downstream of mTOR. BSMCs plated on collagen type I Bioflex plates were serum starved for 48 hours before stimulating with static strain and/or hypoxia. S6K phosphorylation synergistically increased in response

    Journal: The American Journal of Pathology

    Article Title: Mammalian Target of Rapamycin (mTOR) Induces Proliferation and De-Differentiation Responses to Three Coordinate Pathophysiologic Stimuli (Mechanical Strain, Hypoxia, and Extracellular Matrix Remodeling) in Rat Bladder Smooth Muscle

    doi: 10.2353/ajpath.2010.080834

    Figure Lengend Snippet: Mechanical strain and hypoxia activates S6K downstream of mTOR. BSMCs plated on collagen type I Bioflex plates were serum starved for 48 hours before stimulating with static strain and/or hypoxia. S6K phosphorylation synergistically increased in response

    Article Snippet: For proliferation assays, 0.5 × 105 cells were seeded into 6 well tissue culture plates or BioFlex plates (Flexcell International, Inc.).

    Techniques:

    Rapamycin reduces Mmp7 expression induced by mitogenic stimuli. Real-time PCR was performed on cDNA from BSMC, plated on Bioflex plates that were serum-starved and stimulated by 5% equibiaxial strain ± 1% O 2 hypoxia, for 18 hours.

    Journal: The American Journal of Pathology

    Article Title: Mammalian Target of Rapamycin (mTOR) Induces Proliferation and De-Differentiation Responses to Three Coordinate Pathophysiologic Stimuli (Mechanical Strain, Hypoxia, and Extracellular Matrix Remodeling) in Rat Bladder Smooth Muscle

    doi: 10.2353/ajpath.2010.080834

    Figure Lengend Snippet: Rapamycin reduces Mmp7 expression induced by mitogenic stimuli. Real-time PCR was performed on cDNA from BSMC, plated on Bioflex plates that were serum-starved and stimulated by 5% equibiaxial strain ± 1% O 2 hypoxia, for 18 hours.

    Article Snippet: For proliferation assays, 0.5 × 105 cells were seeded into 6 well tissue culture plates or BioFlex plates (Flexcell International, Inc.).

    Techniques: Expressing, Real-time Polymerase Chain Reaction