significant biofilm inhibition activity against mrsa atcc 33591  (ATCC)

Journal: Biofilm

Article Title: Influence of the ATP-dependent DNA ligase, Lig E, on Neisseria gonorrhoeae microcolony and biofilm formation

doi: 10.1016/j.bioflm.2025.100292

Figure Lengend Snippet: Schematic of the set up used to generate N. gonorrhoeae biofilms using the CBR 90 Standard CDC Biofilm Reactor® (Biosurface Technologies). Created in https://BioRender.com .

Article Snippet: We provide the first reported use of the Centre for Disease Control (CDC) Biofilm Reactor® (BioSurface Technologies) to generate constant shear forces during growth of N. gonorrhoeae biofilms, in conjunction with subsequent assessment of biofilms using confocal laser scanning microscopy (CLSM).

Techniques:

Journal: Biofilm

Article Title: Influence of the ATP-dependent DNA ligase, Lig E, on Neisseria gonorrhoeae microcolony and biofilm formation

doi: 10.1016/j.bioflm.2025.100292

Figure Lengend Snippet: Biofilm formation and adhesion of N. gonorrhoeae pEG2 (expressing sfGFP) on polycarbonate coupons in CDC Biofilm Reactors® (a) Representative CLSM z-stack images (x40 objective magnification, em:480 nm, exc:505 nm). Five fields-of-view were imaged for each of the three biological replicates. Additional supporting images can be found in (b) Biomass (c) Surface to biovolume ratio (d) Dimensionless roughness coefficient. (e) Average thickness (entire area) (f) Average thickness (biomass) (g) Maximum thickness. Parameters were calculated using the COMSTAT 2.1 software [ , ]. Points in the bar graphs are the mean of values from the five fields-of-view of three biological replicates and error bars represent the standard error of the mean. Significance values are given as ∗ p ≤ 0.05; ∗∗p ≤ 0.01; ∗∗∗p ≤ 0.001; ∗∗ ∗∗p ≤ 0.0001. Comparisons which showed no significant differences (p > 0.05) are not indicated.

Article Snippet: We provide the first reported use of the Centre for Disease Control (CDC) Biofilm Reactor® (BioSurface Technologies) to generate constant shear forces during growth of N. gonorrhoeae biofilms, in conjunction with subsequent assessment of biofilms using confocal laser scanning microscopy (CLSM).

Techniques: Expressing, Software

Journal: iScience

Article Title: Optimized alveolar epithelial cell model for chronic Pseudomonas aeruginosa and Staphylococcus aureus coinfections

doi: 10.1016/j.isci.2025.113620

Figure Lengend Snippet: Expression of virulence ( exoS , toxA , and vgrG ) and biofilm-related ( wspR , bfiR , and pelA ) genes Fold changes are expressed in comparison to their control (stationary planktonic bacteria in a non-infecting state, without the presence of A549 cells) obtained by RT‒qPCR analysis. 2D refers to the c ollagen coating model, while 3D to the 3D collagen model. The different strains are indicated as follows: P. aeruginosa PAO1 (PAO1) and P. aeruginosa PAET1 (PAET1). Data are presented as mean ± SD. Data between models was compared by one-way ANOVA analysis with a Tukey’s multiple comparisons test (represented in dashed lines). In addition, each gene was compared to its own control with an unpaired t test (∗∗, p ≤ 0.01; ∗∗∗, p < 0.001; ∗∗∗∗, p ≤ 0.0001).

Article Snippet: Scale bar corresponds to 10 μm. (C) Reconstruction of P. aeruginosa PAET1 single and dual biofilms in Imaris Cell Imaging Software (version 7.4.2).

Techniques: Expressing, Comparison, Control, Bacteria

Journal: iScience

Article Title: Optimized alveolar epithelial cell model for chronic Pseudomonas aeruginosa and Staphylococcus aureus coinfections

doi: 10.1016/j.isci.2025.113620

Figure Lengend Snippet: Formation of P. aeruginosa PAET1 biofilm structure on the collagen coating model P. aeruginosa PAET1 single (A) and dual (with S. aureus) (B) biofilms on A549 alveolar cells. The left images correspond to the composite of the sum of slices (z stack), with the respective orthogonal views. The other three images are taken from a z axis analysis of the z stack composite, from the bottom to the surface, to observe better the location of eukaryotic cells and bacteria, respectively. Alveolar cells are shown in blue, S. aureus (SA) in green, and P. aeruginosa PAET1 (PAET1) in red. Scale bar corresponds to 10 μm. (C) Reconstruction of P. aeruginosa PAET1 single and dual biofilms in Imaris Cell Imaging Software (version 7.4.2).

Article Snippet: Scale bar corresponds to 10 μm. (C) Reconstruction of P. aeruginosa PAET1 single and dual biofilms in Imaris Cell Imaging Software (version 7.4.2).

Techniques: Bacteria, Imaging, Software