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Becton Dickinson biofilm formation biofilms
Confocal Laser Scanning Microscopy of <t>biofilm</t> formed by S. pseudintermedius strain DSM 25713. Biofilm was allowed to form for 48 h at 37 °C, in absence of serum, under both ( a ) static, and ( b ) dynamic (flow cell chamber) conditions. Static <t>biofilms</t> were further treated for 24 h with increasing gentamicin concentrations (1x-128xMIC). Representative images of biofilm exposed at ( c ) 1x and ( d ) 128xMIC gentamicin are shown. Orthogonal images z are projections of x and y planes, collected within the biofilm as indicated by the green and red lines in the top view. Image capture was set for simultaneous visualization of red (Propidium iodide-stained dead cells), green (Syto-9-stained viable cells), and blue (Concanavalin A-stained EPS) fluorescence. Magnification, x100
Biofilm Formation Biofilms, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "New insights in Staphylococcus pseudintermedius pathogenicity: antibiotic-resistant biofilm formation by a human wound-associated strain"

Article Title: New insights in Staphylococcus pseudintermedius pathogenicity: antibiotic-resistant biofilm formation by a human wound-associated strain

Journal: BMC Microbiology

doi: 10.1186/s12866-015-0449-x

Confocal Laser Scanning Microscopy of biofilm formed by S. pseudintermedius strain DSM 25713. Biofilm was allowed to form for 48 h at 37 °C, in absence of serum, under both ( a ) static, and ( b ) dynamic (flow cell chamber) conditions. Static biofilms were further treated for 24 h with increasing gentamicin concentrations (1x-128xMIC). Representative images of biofilm exposed at ( c ) 1x and ( d ) 128xMIC gentamicin are shown. Orthogonal images z are projections of x and y planes, collected within the biofilm as indicated by the green and red lines in the top view. Image capture was set for simultaneous visualization of red (Propidium iodide-stained dead cells), green (Syto-9-stained viable cells), and blue (Concanavalin A-stained EPS) fluorescence. Magnification, x100
Figure Legend Snippet: Confocal Laser Scanning Microscopy of biofilm formed by S. pseudintermedius strain DSM 25713. Biofilm was allowed to form for 48 h at 37 °C, in absence of serum, under both ( a ) static, and ( b ) dynamic (flow cell chamber) conditions. Static biofilms were further treated for 24 h with increasing gentamicin concentrations (1x-128xMIC). Representative images of biofilm exposed at ( c ) 1x and ( d ) 128xMIC gentamicin are shown. Orthogonal images z are projections of x and y planes, collected within the biofilm as indicated by the green and red lines in the top view. Image capture was set for simultaneous visualization of red (Propidium iodide-stained dead cells), green (Syto-9-stained viable cells), and blue (Concanavalin A-stained EPS) fluorescence. Magnification, x100

Techniques Used: Confocal Laser Scanning Microscopy, Flow Cytometry, Staining, Fluorescence

Kinetic of biofilm formation, through 72 h-incubation, by S. pseudintermedius strain DSM 25713 onto polystyrene. ( a - f ) Representative SEM images of biofilm formation after 1, 4, 8, 24, 48, and 72 h of incubation, respectively. Magnification (x1.000). ( g , h ) Magnification (x20.000) of ( e ) and ( f ), respectively. Cocci are surrounded by EPS appearing as an extensive network of filaments stretched among cells and between these and the substratum. ( i ) Kinetic of biofilm formation as assessed by viable count. Maximum, median, and minimum values are shown in each box (n = 6)
Figure Legend Snippet: Kinetic of biofilm formation, through 72 h-incubation, by S. pseudintermedius strain DSM 25713 onto polystyrene. ( a - f ) Representative SEM images of biofilm formation after 1, 4, 8, 24, 48, and 72 h of incubation, respectively. Magnification (x1.000). ( g , h ) Magnification (x20.000) of ( e ) and ( f ), respectively. Cocci are surrounded by EPS appearing as an extensive network of filaments stretched among cells and between these and the substratum. ( i ) Kinetic of biofilm formation as assessed by viable count. Maximum, median, and minimum values are shown in each box (n = 6)

Techniques Used: Incubation

ESEM images of biofilm formed by S. pseudintermedius strain DSM 25713 onto polystyrene following 72 h-incubation. ( a ) Biofilm exhibited spatially heterogeneous organization, as suggested by the presence of “mushroom-like” structures (as indicated by arrows). Magnification: x3.000. ( b , c ) Multilayered organization with the presence of bacteria under EPS matrix (as indicated by arrows). Magnification: x12.500 and x20.000, respectively
Figure Legend Snippet: ESEM images of biofilm formed by S. pseudintermedius strain DSM 25713 onto polystyrene following 72 h-incubation. ( a ) Biofilm exhibited spatially heterogeneous organization, as suggested by the presence of “mushroom-like” structures (as indicated by arrows). Magnification: x3.000. ( b , c ) Multilayered organization with the presence of bacteria under EPS matrix (as indicated by arrows). Magnification: x12.500 and x20.000, respectively

Techniques Used: Incubation

Standardization of experimental conditions for biofilm formation by S. pseudintermedius strain DSM 25713 on polystyrene surface. Effect of dynamic (filled squares) or static (filled triangles) incubation, incubation time (24, 48, and 72 h), and inoculum concentration (10 5 , 10 6 , and 10 7 CFU/mL) on biofilm biomass formation, as assessed by spectrophotometric assay. Values are means ± SDs (n = 6). *** p
Figure Legend Snippet: Standardization of experimental conditions for biofilm formation by S. pseudintermedius strain DSM 25713 on polystyrene surface. Effect of dynamic (filled squares) or static (filled triangles) incubation, incubation time (24, 48, and 72 h), and inoculum concentration (10 5 , 10 6 , and 10 7 CFU/mL) on biofilm biomass formation, as assessed by spectrophotometric assay. Values are means ± SDs (n = 6). *** p

Techniques Used: Incubation, Concentration Assay, Spectrophotometric Assay

Effect of serum and pH on biofilm formation and growth by S. pseudintermedius strain DSM 25713. ( a ) Serum was tested against biofilm formation at various dilutions (1:2, 1:10, and 1:100), as free or adsorbed to polystyrene, under different pH (5.5, 7.1, and 8.7). Control wells contained bacteria but not serum. Biofilm biomass amount was measured by crystal violet assay, then normalized on bacterial growth by calculating the specific biofilm formation (SBF) index (see Materials and Methods). ( b ) The effect of free serum against bacterial growth was assessed by measuring OD 600 of cell grown in broth following 24 h-incubation. Results are means + SDs (n = 9). * p
Figure Legend Snippet: Effect of serum and pH on biofilm formation and growth by S. pseudintermedius strain DSM 25713. ( a ) Serum was tested against biofilm formation at various dilutions (1:2, 1:10, and 1:100), as free or adsorbed to polystyrene, under different pH (5.5, 7.1, and 8.7). Control wells contained bacteria but not serum. Biofilm biomass amount was measured by crystal violet assay, then normalized on bacterial growth by calculating the specific biofilm formation (SBF) index (see Materials and Methods). ( b ) The effect of free serum against bacterial growth was assessed by measuring OD 600 of cell grown in broth following 24 h-incubation. Results are means + SDs (n = 9). * p

Techniques Used: Crystal Violet Assay, Incubation

In vitro effect of antibiotics against preformed biofilm by S. pseudintermedius strain DSM 25713. Biofilms allowed to form following 48 h-incubation were exposed for further 24 h to each antibiotic at concentrations equal or multiple of MIC. Results are expressed as percentage of biofilm’s viability – as assessed by viable colony count - compared to control (unexposed, 100 % viability) (n = 6). The dotted line indicates a reduction in biofilm viability of at least 20 % vs control ( p
Figure Legend Snippet: In vitro effect of antibiotics against preformed biofilm by S. pseudintermedius strain DSM 25713. Biofilms allowed to form following 48 h-incubation were exposed for further 24 h to each antibiotic at concentrations equal or multiple of MIC. Results are expressed as percentage of biofilm’s viability – as assessed by viable colony count - compared to control (unexposed, 100 % viability) (n = 6). The dotted line indicates a reduction in biofilm viability of at least 20 % vs control ( p

Techniques Used: In Vitro, Incubation

In vitro activity of antibiotics at sub-inhibitory concentrations against biofilm formation by S. pseudintermedius strain DSM 25713. Biofilm biomass formed during 24 h-incubation was measured, using the crystal violet assay, in the presence of antibiotics at concentrations equal to 1/2x, 1/4x, and 1/8xMIC. Results were plotted as percentage of biofilm biomass formed in the presence of antibiotic, compared to controls (not exposed, 100 % biofilm biomass) (n = 6). The dotted line indicates a reduction in biofilm biomass of at least 20 % vs control ( p
Figure Legend Snippet: In vitro activity of antibiotics at sub-inhibitory concentrations against biofilm formation by S. pseudintermedius strain DSM 25713. Biofilm biomass formed during 24 h-incubation was measured, using the crystal violet assay, in the presence of antibiotics at concentrations equal to 1/2x, 1/4x, and 1/8xMIC. Results were plotted as percentage of biofilm biomass formed in the presence of antibiotic, compared to controls (not exposed, 100 % biofilm biomass) (n = 6). The dotted line indicates a reduction in biofilm biomass of at least 20 % vs control ( p

Techniques Used: In Vitro, Activity Assay, Incubation, Crystal Violet Assay

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    Becton Dickinson biofilm formation kinetics
    Confocal Laser Scanning Microscopy of <t>biofilm</t> formed by S. pseudintermedius strain DSM 25713. Biofilm was allowed to form for 48 h at 37 °C, in absence of serum, under both ( a ) static, and ( b ) dynamic (flow cell chamber) conditions. Static biofilms were further treated for 24 h with increasing gentamicin concentrations (1x-128xMIC). Representative images of biofilm exposed at ( c ) 1x and ( d ) 128xMIC gentamicin are shown. Orthogonal images z are projections of x and y planes, collected within the biofilm as indicated by the green and red lines in the top view. Image capture was set for simultaneous visualization of red (Propidium iodide-stained dead cells), green (Syto-9-stained viable cells), and blue (Concanavalin A-stained EPS) fluorescence. Magnification, x100
    Biofilm Formation Kinetics, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biofilm formation kinetics/product/Becton Dickinson
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    biofilm formation kinetics - by Bioz Stars, 2020-05
    88/100 stars
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    Confocal Laser Scanning Microscopy of biofilm formed by S. pseudintermedius strain DSM 25713. Biofilm was allowed to form for 48 h at 37 °C, in absence of serum, under both ( a ) static, and ( b ) dynamic (flow cell chamber) conditions. Static biofilms were further treated for 24 h with increasing gentamicin concentrations (1x-128xMIC). Representative images of biofilm exposed at ( c ) 1x and ( d ) 128xMIC gentamicin are shown. Orthogonal images z are projections of x and y planes, collected within the biofilm as indicated by the green and red lines in the top view. Image capture was set for simultaneous visualization of red (Propidium iodide-stained dead cells), green (Syto-9-stained viable cells), and blue (Concanavalin A-stained EPS) fluorescence. Magnification, x100

    Journal: BMC Microbiology

    Article Title: New insights in Staphylococcus pseudintermedius pathogenicity: antibiotic-resistant biofilm formation by a human wound-associated strain

    doi: 10.1186/s12866-015-0449-x

    Figure Lengend Snippet: Confocal Laser Scanning Microscopy of biofilm formed by S. pseudintermedius strain DSM 25713. Biofilm was allowed to form for 48 h at 37 °C, in absence of serum, under both ( a ) static, and ( b ) dynamic (flow cell chamber) conditions. Static biofilms were further treated for 24 h with increasing gentamicin concentrations (1x-128xMIC). Representative images of biofilm exposed at ( c ) 1x and ( d ) 128xMIC gentamicin are shown. Orthogonal images z are projections of x and y planes, collected within the biofilm as indicated by the green and red lines in the top view. Image capture was set for simultaneous visualization of red (Propidium iodide-stained dead cells), green (Syto-9-stained viable cells), and blue (Concanavalin A-stained EPS) fluorescence. Magnification, x100

    Article Snippet: Biofilm formation kinetics in TSB was monitored - under static conditions, without serum, at 37 °C, and at pH 7.1 - in 35 mm-tissue culture polystyrene dish (Becton, Dickinson and Company) at different time periods (30 min, 1, 2, 4, 8, 24, 48, and 72 h).

    Techniques: Confocal Laser Scanning Microscopy, Flow Cytometry, Staining, Fluorescence

    Kinetic of biofilm formation, through 72 h-incubation, by S. pseudintermedius strain DSM 25713 onto polystyrene. ( a - f ) Representative SEM images of biofilm formation after 1, 4, 8, 24, 48, and 72 h of incubation, respectively. Magnification (x1.000). ( g , h ) Magnification (x20.000) of ( e ) and ( f ), respectively. Cocci are surrounded by EPS appearing as an extensive network of filaments stretched among cells and between these and the substratum. ( i ) Kinetic of biofilm formation as assessed by viable count. Maximum, median, and minimum values are shown in each box (n = 6)

    Journal: BMC Microbiology

    Article Title: New insights in Staphylococcus pseudintermedius pathogenicity: antibiotic-resistant biofilm formation by a human wound-associated strain

    doi: 10.1186/s12866-015-0449-x

    Figure Lengend Snippet: Kinetic of biofilm formation, through 72 h-incubation, by S. pseudintermedius strain DSM 25713 onto polystyrene. ( a - f ) Representative SEM images of biofilm formation after 1, 4, 8, 24, 48, and 72 h of incubation, respectively. Magnification (x1.000). ( g , h ) Magnification (x20.000) of ( e ) and ( f ), respectively. Cocci are surrounded by EPS appearing as an extensive network of filaments stretched among cells and between these and the substratum. ( i ) Kinetic of biofilm formation as assessed by viable count. Maximum, median, and minimum values are shown in each box (n = 6)

    Article Snippet: Biofilm formation kinetics in TSB was monitored - under static conditions, without serum, at 37 °C, and at pH 7.1 - in 35 mm-tissue culture polystyrene dish (Becton, Dickinson and Company) at different time periods (30 min, 1, 2, 4, 8, 24, 48, and 72 h).

    Techniques: Incubation

    ESEM images of biofilm formed by S. pseudintermedius strain DSM 25713 onto polystyrene following 72 h-incubation. ( a ) Biofilm exhibited spatially heterogeneous organization, as suggested by the presence of “mushroom-like” structures (as indicated by arrows). Magnification: x3.000. ( b , c ) Multilayered organization with the presence of bacteria under EPS matrix (as indicated by arrows). Magnification: x12.500 and x20.000, respectively

    Journal: BMC Microbiology

    Article Title: New insights in Staphylococcus pseudintermedius pathogenicity: antibiotic-resistant biofilm formation by a human wound-associated strain

    doi: 10.1186/s12866-015-0449-x

    Figure Lengend Snippet: ESEM images of biofilm formed by S. pseudintermedius strain DSM 25713 onto polystyrene following 72 h-incubation. ( a ) Biofilm exhibited spatially heterogeneous organization, as suggested by the presence of “mushroom-like” structures (as indicated by arrows). Magnification: x3.000. ( b , c ) Multilayered organization with the presence of bacteria under EPS matrix (as indicated by arrows). Magnification: x12.500 and x20.000, respectively

    Article Snippet: Biofilm formation kinetics in TSB was monitored - under static conditions, without serum, at 37 °C, and at pH 7.1 - in 35 mm-tissue culture polystyrene dish (Becton, Dickinson and Company) at different time periods (30 min, 1, 2, 4, 8, 24, 48, and 72 h).

    Techniques: Incubation

    Standardization of experimental conditions for biofilm formation by S. pseudintermedius strain DSM 25713 on polystyrene surface. Effect of dynamic (filled squares) or static (filled triangles) incubation, incubation time (24, 48, and 72 h), and inoculum concentration (10 5 , 10 6 , and 10 7 CFU/mL) on biofilm biomass formation, as assessed by spectrophotometric assay. Values are means ± SDs (n = 6). *** p

    Journal: BMC Microbiology

    Article Title: New insights in Staphylococcus pseudintermedius pathogenicity: antibiotic-resistant biofilm formation by a human wound-associated strain

    doi: 10.1186/s12866-015-0449-x

    Figure Lengend Snippet: Standardization of experimental conditions for biofilm formation by S. pseudintermedius strain DSM 25713 on polystyrene surface. Effect of dynamic (filled squares) or static (filled triangles) incubation, incubation time (24, 48, and 72 h), and inoculum concentration (10 5 , 10 6 , and 10 7 CFU/mL) on biofilm biomass formation, as assessed by spectrophotometric assay. Values are means ± SDs (n = 6). *** p

    Article Snippet: Biofilm formation kinetics in TSB was monitored - under static conditions, without serum, at 37 °C, and at pH 7.1 - in 35 mm-tissue culture polystyrene dish (Becton, Dickinson and Company) at different time periods (30 min, 1, 2, 4, 8, 24, 48, and 72 h).

    Techniques: Incubation, Concentration Assay, Spectrophotometric Assay

    Effect of serum and pH on biofilm formation and growth by S. pseudintermedius strain DSM 25713. ( a ) Serum was tested against biofilm formation at various dilutions (1:2, 1:10, and 1:100), as free or adsorbed to polystyrene, under different pH (5.5, 7.1, and 8.7). Control wells contained bacteria but not serum. Biofilm biomass amount was measured by crystal violet assay, then normalized on bacterial growth by calculating the specific biofilm formation (SBF) index (see Materials and Methods). ( b ) The effect of free serum against bacterial growth was assessed by measuring OD 600 of cell grown in broth following 24 h-incubation. Results are means + SDs (n = 9). * p

    Journal: BMC Microbiology

    Article Title: New insights in Staphylococcus pseudintermedius pathogenicity: antibiotic-resistant biofilm formation by a human wound-associated strain

    doi: 10.1186/s12866-015-0449-x

    Figure Lengend Snippet: Effect of serum and pH on biofilm formation and growth by S. pseudintermedius strain DSM 25713. ( a ) Serum was tested against biofilm formation at various dilutions (1:2, 1:10, and 1:100), as free or adsorbed to polystyrene, under different pH (5.5, 7.1, and 8.7). Control wells contained bacteria but not serum. Biofilm biomass amount was measured by crystal violet assay, then normalized on bacterial growth by calculating the specific biofilm formation (SBF) index (see Materials and Methods). ( b ) The effect of free serum against bacterial growth was assessed by measuring OD 600 of cell grown in broth following 24 h-incubation. Results are means + SDs (n = 9). * p

    Article Snippet: Biofilm formation kinetics in TSB was monitored - under static conditions, without serum, at 37 °C, and at pH 7.1 - in 35 mm-tissue culture polystyrene dish (Becton, Dickinson and Company) at different time periods (30 min, 1, 2, 4, 8, 24, 48, and 72 h).

    Techniques: Crystal Violet Assay, Incubation

    In vitro effect of antibiotics against preformed biofilm by S. pseudintermedius strain DSM 25713. Biofilms allowed to form following 48 h-incubation were exposed for further 24 h to each antibiotic at concentrations equal or multiple of MIC. Results are expressed as percentage of biofilm’s viability – as assessed by viable colony count - compared to control (unexposed, 100 % viability) (n = 6). The dotted line indicates a reduction in biofilm viability of at least 20 % vs control ( p

    Journal: BMC Microbiology

    Article Title: New insights in Staphylococcus pseudintermedius pathogenicity: antibiotic-resistant biofilm formation by a human wound-associated strain

    doi: 10.1186/s12866-015-0449-x

    Figure Lengend Snippet: In vitro effect of antibiotics against preformed biofilm by S. pseudintermedius strain DSM 25713. Biofilms allowed to form following 48 h-incubation were exposed for further 24 h to each antibiotic at concentrations equal or multiple of MIC. Results are expressed as percentage of biofilm’s viability – as assessed by viable colony count - compared to control (unexposed, 100 % viability) (n = 6). The dotted line indicates a reduction in biofilm viability of at least 20 % vs control ( p

    Article Snippet: Biofilm formation kinetics in TSB was monitored - under static conditions, without serum, at 37 °C, and at pH 7.1 - in 35 mm-tissue culture polystyrene dish (Becton, Dickinson and Company) at different time periods (30 min, 1, 2, 4, 8, 24, 48, and 72 h).

    Techniques: In Vitro, Incubation

    In vitro activity of antibiotics at sub-inhibitory concentrations against biofilm formation by S. pseudintermedius strain DSM 25713. Biofilm biomass formed during 24 h-incubation was measured, using the crystal violet assay, in the presence of antibiotics at concentrations equal to 1/2x, 1/4x, and 1/8xMIC. Results were plotted as percentage of biofilm biomass formed in the presence of antibiotic, compared to controls (not exposed, 100 % biofilm biomass) (n = 6). The dotted line indicates a reduction in biofilm biomass of at least 20 % vs control ( p

    Journal: BMC Microbiology

    Article Title: New insights in Staphylococcus pseudintermedius pathogenicity: antibiotic-resistant biofilm formation by a human wound-associated strain

    doi: 10.1186/s12866-015-0449-x

    Figure Lengend Snippet: In vitro activity of antibiotics at sub-inhibitory concentrations against biofilm formation by S. pseudintermedius strain DSM 25713. Biofilm biomass formed during 24 h-incubation was measured, using the crystal violet assay, in the presence of antibiotics at concentrations equal to 1/2x, 1/4x, and 1/8xMIC. Results were plotted as percentage of biofilm biomass formed in the presence of antibiotic, compared to controls (not exposed, 100 % biofilm biomass) (n = 6). The dotted line indicates a reduction in biofilm biomass of at least 20 % vs control ( p

    Article Snippet: Biofilm formation kinetics in TSB was monitored - under static conditions, without serum, at 37 °C, and at pH 7.1 - in 35 mm-tissue culture polystyrene dish (Becton, Dickinson and Company) at different time periods (30 min, 1, 2, 4, 8, 24, 48, and 72 h).

    Techniques: In Vitro, Activity Assay, Incubation, Crystal Violet Assay

    SEM micrographs of S. epidermidis ATCC 35984 biofilm formation on glass surfaces. Biofilms were grown in TSBGlc (a) or in TSBGlc supplemented with THR-SK004 ethanol extract at 250, (b) and 500 μ g/mL, (c), and all images shown were taken at magnification 2500x. The selected images were chosen as the best representatives of the amount of biofilm on the glass surfaces. Inhibition of staphylococcal biofilm development by THR-SK004 ethanol extract was additionally confirmed by crystal violet assay (d).

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Inhibition of Staphylococcus epidermidis Biofilm Formation by Traditional Thai Herbal Recipes Used for Wound Treatment

    doi: 10.1155/2012/159797

    Figure Lengend Snippet: SEM micrographs of S. epidermidis ATCC 35984 biofilm formation on glass surfaces. Biofilms were grown in TSBGlc (a) or in TSBGlc supplemented with THR-SK004 ethanol extract at 250, (b) and 500 μ g/mL, (c), and all images shown were taken at magnification 2500x. The selected images were chosen as the best representatives of the amount of biofilm on the glass surfaces. Inhibition of staphylococcal biofilm development by THR-SK004 ethanol extract was additionally confirmed by crystal violet assay (d).

    Article Snippet: In order to assess the biofilm formation ability of Staphylococcus spp., well-isolated colonies grown overnight at 37°C on tryptic soy agar (TSA, Becton, Dickinson, and Company, France) were inoculated in tryptic soy broth (TSB, Becton, Dickinson, and Company) supplemented with 2% (w/w) D-glucose (TSBGlc).

    Techniques: Inhibition, Crystal Violet Assay

    Time-dependent eradication of the mature biofilm formed by S. epidermidis ATCC 35984 after treatment with THR-SK004 ethanol extract (a) at 250 ( ⚫ ) and 500 μ g/mL (■) or THR-SK010 ethanol extract (b) at 10 μ g/mL ( ⚫ ) and 20 μ g/mL (■). Dimethylsulfoxide at 0.2% (o) was used as positive control. Each symbol indicates the means ± standard error for three independent experiments performed in duplicate.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Inhibition of Staphylococcus epidermidis Biofilm Formation by Traditional Thai Herbal Recipes Used for Wound Treatment

    doi: 10.1155/2012/159797

    Figure Lengend Snippet: Time-dependent eradication of the mature biofilm formed by S. epidermidis ATCC 35984 after treatment with THR-SK004 ethanol extract (a) at 250 ( ⚫ ) and 500 μ g/mL (■) or THR-SK010 ethanol extract (b) at 10 μ g/mL ( ⚫ ) and 20 μ g/mL (■). Dimethylsulfoxide at 0.2% (o) was used as positive control. Each symbol indicates the means ± standard error for three independent experiments performed in duplicate.

    Article Snippet: In order to assess the biofilm formation ability of Staphylococcus spp., well-isolated colonies grown overnight at 37°C on tryptic soy agar (TSA, Becton, Dickinson, and Company, France) were inoculated in tryptic soy broth (TSB, Becton, Dickinson, and Company) supplemented with 2% (w/w) D-glucose (TSBGlc).

    Techniques: Positive Control

    Effect of different concentrations of THR-SK004 (a), THR-SK010 (b), and THR-SK011 (c) ethanol (- ⚫ -,■), and water (-o-, □) extracts on the bacterial growth (linear charts) and the biofilm formation (column charts) of Staphylococcus epidermidis ATCC 35984.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Inhibition of Staphylococcus epidermidis Biofilm Formation by Traditional Thai Herbal Recipes Used for Wound Treatment

    doi: 10.1155/2012/159797

    Figure Lengend Snippet: Effect of different concentrations of THR-SK004 (a), THR-SK010 (b), and THR-SK011 (c) ethanol (- ⚫ -,■), and water (-o-, □) extracts on the bacterial growth (linear charts) and the biofilm formation (column charts) of Staphylococcus epidermidis ATCC 35984.

    Article Snippet: In order to assess the biofilm formation ability of Staphylococcus spp., well-isolated colonies grown overnight at 37°C on tryptic soy agar (TSA, Becton, Dickinson, and Company, France) were inoculated in tryptic soy broth (TSB, Becton, Dickinson, and Company) supplemented with 2% (w/w) D-glucose (TSBGlc).

    Techniques:

    Development of Staphylococcus epidermidis ATCC 35984 biofilm (column charts) and the bacterial growth (linear charts) after treatment with THR-SK004 ethanol extract at 125 (grey symbols) and 250 μ g/mL (black symbols). Dimethylsulfoxide at 0.2% (white symbols) was used as positive control. Each symbol indicates the means ± standard error for three independent experiments performed in duplicate.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Inhibition of Staphylococcus epidermidis Biofilm Formation by Traditional Thai Herbal Recipes Used for Wound Treatment

    doi: 10.1155/2012/159797

    Figure Lengend Snippet: Development of Staphylococcus epidermidis ATCC 35984 biofilm (column charts) and the bacterial growth (linear charts) after treatment with THR-SK004 ethanol extract at 125 (grey symbols) and 250 μ g/mL (black symbols). Dimethylsulfoxide at 0.2% (white symbols) was used as positive control. Each symbol indicates the means ± standard error for three independent experiments performed in duplicate.

    Article Snippet: In order to assess the biofilm formation ability of Staphylococcus spp., well-isolated colonies grown overnight at 37°C on tryptic soy agar (TSA, Becton, Dickinson, and Company, France) were inoculated in tryptic soy broth (TSB, Becton, Dickinson, and Company) supplemented with 2% (w/w) D-glucose (TSBGlc).

    Techniques: Positive Control