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Corning Life Sciences biofilm construction biofilms
Viable counts of each bacterial species present in the initial inoculum (per well) and in the 3‐day old <t>biofilms</t> ( n = 7). Fn = Fusobacterium nucleatum ; Pg = Porphyromonas gingivalis ; Aa = Aggregatibacter actinomycetemcomitans . Error bars: standard error
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Article Title: The effect of metronidazole plus amoxicillin or metronidazole plus penicillin V on periodontal pathogens in an in vitro biofilm model. The effect of metronidazole plus amoxicillin or metronidazole plus penicillin V on periodontal pathogens in an in vitro biofilm model

Journal: Clinical and Experimental Dental Research

doi: 10.1002/cre2.96

Viable counts of each bacterial species present in the initial inoculum (per well) and in the 3‐day old biofilms ( n = 7). Fn = Fusobacterium nucleatum ; Pg = Porphyromonas gingivalis ; Aa = Aggregatibacter actinomycetemcomitans . Error bars: standard error
Figure Legend Snippet: Viable counts of each bacterial species present in the initial inoculum (per well) and in the 3‐day old biofilms ( n = 7). Fn = Fusobacterium nucleatum ; Pg = Porphyromonas gingivalis ; Aa = Aggregatibacter actinomycetemcomitans . Error bars: standard error

Techniques Used:

CLSM images of 3‐day old biofilm after antibiotic exposure. Overlapping of images collected from green and red channel. (a) 3‐day‐old biofilm (negative control); (b) 3‐day old biofilm CHX‐treated for 2 hr; (c) 3‐day old biofilm treated with AMX + MET in high concentration for 2 hr; (d) 3‐day old biofilm treated with PV + MET in high concentration for 2 hr. Z‐stacks were taken in xyz projection with 63× objective, oil immersion, at 10 μm from the biofilm bottom. Scale bar: 10 μm
Figure Legend Snippet: CLSM images of 3‐day old biofilm after antibiotic exposure. Overlapping of images collected from green and red channel. (a) 3‐day‐old biofilm (negative control); (b) 3‐day old biofilm CHX‐treated for 2 hr; (c) 3‐day old biofilm treated with AMX + MET in high concentration for 2 hr; (d) 3‐day old biofilm treated with PV + MET in high concentration for 2 hr. Z‐stacks were taken in xyz projection with 63× objective, oil immersion, at 10 μm from the biofilm bottom. Scale bar: 10 μm

Techniques Used: Confocal Laser Scanning Microscopy, Negative Control, Concentration Assay

Viable counts of each bacterial species in the 3‐day old biofilms (negative controls) and subjected to antiseptic or antibiotic treatment ( n = 6). No live CFU were retrieved from biofilms treated with CHX (positive controls). Single green bars: number of live Aggregatibacter actinomycetemcomitans retrieved from biofilms exposed to antibiotic combinations in high (H) or low (L) concentrations. * shows statistical significance between biofilms exposed to PV + MET in high and low concentration ( p = .041, t test). Fn = Fusobacterium nucleatum ; Pg = Porphyromonas gingivalis ; Aa = A . actinomycetemcomitans . Error bars: standard error
Figure Legend Snippet: Viable counts of each bacterial species in the 3‐day old biofilms (negative controls) and subjected to antiseptic or antibiotic treatment ( n = 6). No live CFU were retrieved from biofilms treated with CHX (positive controls). Single green bars: number of live Aggregatibacter actinomycetemcomitans retrieved from biofilms exposed to antibiotic combinations in high (H) or low (L) concentrations. * shows statistical significance between biofilms exposed to PV + MET in high and low concentration ( p = .041, t test). Fn = Fusobacterium nucleatum ; Pg = Porphyromonas gingivalis ; Aa = A . actinomycetemcomitans . Error bars: standard error

Techniques Used: Concentration Assay

CLSM image in maximum projection of the series taken in xzy axis of the 3‐day old biofilm. Viable and nonviable bacterial cells are depicted in green and red, respectively. Scale bar: 10 μm
Figure Legend Snippet: CLSM image in maximum projection of the series taken in xzy axis of the 3‐day old biofilm. Viable and nonviable bacterial cells are depicted in green and red, respectively. Scale bar: 10 μm

Techniques Used: Confocal Laser Scanning Microscopy

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Article Title: The effect of metronidazole plus amoxicillin or metronidazole plus penicillin V on periodontal pathogens in an in vitro biofilm model. The effect of metronidazole plus amoxicillin or metronidazole plus penicillin V on periodontal pathogens in an in vitro biofilm model
Article Snippet: 2.2 Biofilm construction Biofilms were prepared in 24‐well plates (Corning Inc., NY, USA) by adapting a previously described protocol (Sanchez et al., ).

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    Corning Life Sciences biofilm construction biofilms
    Viable counts of each bacterial species present in the initial inoculum (per well) and in the 3‐day old <t>biofilms</t> ( n = 7). Fn = Fusobacterium nucleatum ; Pg = Porphyromonas gingivalis ; Aa = Aggregatibacter actinomycetemcomitans . Error bars: standard error
    Biofilm Construction Biofilms, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biofilm construction biofilms/product/Corning Life Sciences
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biofilm construction biofilms - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

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    Viable counts of each bacterial species present in the initial inoculum (per well) and in the 3‐day old biofilms ( n = 7). Fn = Fusobacterium nucleatum ; Pg = Porphyromonas gingivalis ; Aa = Aggregatibacter actinomycetemcomitans . Error bars: standard error

    Journal: Clinical and Experimental Dental Research

    Article Title: The effect of metronidazole plus amoxicillin or metronidazole plus penicillin V on periodontal pathogens in an in vitro biofilm model. The effect of metronidazole plus amoxicillin or metronidazole plus penicillin V on periodontal pathogens in an in vitro biofilm model

    doi: 10.1002/cre2.96

    Figure Lengend Snippet: Viable counts of each bacterial species present in the initial inoculum (per well) and in the 3‐day old biofilms ( n = 7). Fn = Fusobacterium nucleatum ; Pg = Porphyromonas gingivalis ; Aa = Aggregatibacter actinomycetemcomitans . Error bars: standard error

    Article Snippet: 2.2 Biofilm construction Biofilms were prepared in 24‐well plates (Corning Inc., NY, USA) by adapting a previously described protocol (Sanchez et al., ).

    Techniques:

    CLSM images of 3‐day old biofilm after antibiotic exposure. Overlapping of images collected from green and red channel. (a) 3‐day‐old biofilm (negative control); (b) 3‐day old biofilm CHX‐treated for 2 hr; (c) 3‐day old biofilm treated with AMX + MET in high concentration for 2 hr; (d) 3‐day old biofilm treated with PV + MET in high concentration for 2 hr. Z‐stacks were taken in xyz projection with 63× objective, oil immersion, at 10 μm from the biofilm bottom. Scale bar: 10 μm

    Journal: Clinical and Experimental Dental Research

    Article Title: The effect of metronidazole plus amoxicillin or metronidazole plus penicillin V on periodontal pathogens in an in vitro biofilm model. The effect of metronidazole plus amoxicillin or metronidazole plus penicillin V on periodontal pathogens in an in vitro biofilm model

    doi: 10.1002/cre2.96

    Figure Lengend Snippet: CLSM images of 3‐day old biofilm after antibiotic exposure. Overlapping of images collected from green and red channel. (a) 3‐day‐old biofilm (negative control); (b) 3‐day old biofilm CHX‐treated for 2 hr; (c) 3‐day old biofilm treated with AMX + MET in high concentration for 2 hr; (d) 3‐day old biofilm treated with PV + MET in high concentration for 2 hr. Z‐stacks were taken in xyz projection with 63× objective, oil immersion, at 10 μm from the biofilm bottom. Scale bar: 10 μm

    Article Snippet: 2.2 Biofilm construction Biofilms were prepared in 24‐well plates (Corning Inc., NY, USA) by adapting a previously described protocol (Sanchez et al., ).

    Techniques: Confocal Laser Scanning Microscopy, Negative Control, Concentration Assay

    Viable counts of each bacterial species in the 3‐day old biofilms (negative controls) and subjected to antiseptic or antibiotic treatment ( n = 6). No live CFU were retrieved from biofilms treated with CHX (positive controls). Single green bars: number of live Aggregatibacter actinomycetemcomitans retrieved from biofilms exposed to antibiotic combinations in high (H) or low (L) concentrations. * shows statistical significance between biofilms exposed to PV + MET in high and low concentration ( p = .041, t test). Fn = Fusobacterium nucleatum ; Pg = Porphyromonas gingivalis ; Aa = A . actinomycetemcomitans . Error bars: standard error

    Journal: Clinical and Experimental Dental Research

    Article Title: The effect of metronidazole plus amoxicillin or metronidazole plus penicillin V on periodontal pathogens in an in vitro biofilm model. The effect of metronidazole plus amoxicillin or metronidazole plus penicillin V on periodontal pathogens in an in vitro biofilm model

    doi: 10.1002/cre2.96

    Figure Lengend Snippet: Viable counts of each bacterial species in the 3‐day old biofilms (negative controls) and subjected to antiseptic or antibiotic treatment ( n = 6). No live CFU were retrieved from biofilms treated with CHX (positive controls). Single green bars: number of live Aggregatibacter actinomycetemcomitans retrieved from biofilms exposed to antibiotic combinations in high (H) or low (L) concentrations. * shows statistical significance between biofilms exposed to PV + MET in high and low concentration ( p = .041, t test). Fn = Fusobacterium nucleatum ; Pg = Porphyromonas gingivalis ; Aa = A . actinomycetemcomitans . Error bars: standard error

    Article Snippet: 2.2 Biofilm construction Biofilms were prepared in 24‐well plates (Corning Inc., NY, USA) by adapting a previously described protocol (Sanchez et al., ).

    Techniques: Concentration Assay

    CLSM image in maximum projection of the series taken in xzy axis of the 3‐day old biofilm. Viable and nonviable bacterial cells are depicted in green and red, respectively. Scale bar: 10 μm

    Journal: Clinical and Experimental Dental Research

    Article Title: The effect of metronidazole plus amoxicillin or metronidazole plus penicillin V on periodontal pathogens in an in vitro biofilm model. The effect of metronidazole plus amoxicillin or metronidazole plus penicillin V on periodontal pathogens in an in vitro biofilm model

    doi: 10.1002/cre2.96

    Figure Lengend Snippet: CLSM image in maximum projection of the series taken in xzy axis of the 3‐day old biofilm. Viable and nonviable bacterial cells are depicted in green and red, respectively. Scale bar: 10 μm

    Article Snippet: 2.2 Biofilm construction Biofilms were prepared in 24‐well plates (Corning Inc., NY, USA) by adapting a previously described protocol (Sanchez et al., ).

    Techniques: Confocal Laser Scanning Microscopy