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Bio-Rad biofilm a
Biofilm A, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: ?1 Soluble Guanylyl Cyclase (sGC) Splice Forms as Potential Regulators of Human sGC Activity *?1 Soluble Guanylyl Cyclase (sGC) Splice Forms as Potential Regulators of Human sGC Activity * S⃞
Article Snippet: .. Each plate was covered with Biofilm A (Bio-Rad) and incubated in a PTC-100 (96) or DYAD (384) thermocycler (Bio-Rad) for 30 min at 50 °C, followed by 72 °C for 10 min. .. Subsequently, 40 μl or 20 μl of a PCR master mix (400 n m forward and reverse primers (IDT, Coralville, IA), 100 n m fluorogenic probe (Biosearch Technologies, Novato, CA), 5 m m MgCl2 , 200 μ m deoxynucleotides, PCR buffer, 150 n m SuperROX dye (Biosearch Technologies, Novato, CA), and 1.25 units of Taq polymerase (Invitrogen) were added directly to each well of the cDNA plate.

Article Title: Pendrin localizes to the adrenal medulla and modulates catecholamine release
Article Snippet: .. Each plate was covered with Biofilm A (Bio-Rad) and incubated in a DYAD (384-well block) thermocycler (Bio-Rad) for 30 min at 50°C followed by 72°C for 10 min. .. Plasma levels of epinephrine and norepinephrine were quantified in 50 μl of plasma by use of reverse-phase liquid chromatography with electrochemical detection after partial purification by adsorption of catechols onto alumina ( ).

Blocking Assay:

Article Title: Pendrin localizes to the adrenal medulla and modulates catecholamine release
Article Snippet: .. Each plate was covered with Biofilm A (Bio-Rad) and incubated in a DYAD (384-well block) thermocycler (Bio-Rad) for 30 min at 50°C followed by 72°C for 10 min. .. Plasma levels of epinephrine and norepinephrine were quantified in 50 μl of plasma by use of reverse-phase liquid chromatography with electrochemical detection after partial purification by adsorption of catechols onto alumina ( ).

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  • 92
    Bio-Rad biofilm
    Confocal microscopy of MRSA (NRS 385) <t>biofilm</t> without treatment ( A ) and after treatment with octenidine hydrochloride ( B ).
    Biofilm, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biofilm/product/Bio-Rad
    Average 92 stars, based on 97 article reviews
    Price from $9.99 to $1999.99
    biofilm - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    90
    Bio-Rad biofilm cellular density biofilm cellular density
    Effect of hLF1-11 on <t>biofilm</t> architecture of C . albicans strains. Inverted microscope images (400× magnification) of (a) C . albicans SC5314 and three clinical isolates, (b) CA688, (c) CA22, and (d) CA37, untreated and treated with 44mg/L, 88mg/L and 176mg/L hLF1-11. C . albicans strains were co-incubated with the different hLF1-11 concentrations at 37°C for 24h.
    Biofilm Cellular Density Biofilm Cellular Density, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biofilm cellular density biofilm cellular density/product/Bio-Rad
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biofilm cellular density biofilm cellular density - by Bioz Stars, 2020-05
    90/100 stars
      Buy from Supplier

    Image Search Results


    Confocal microscopy of MRSA (NRS 385) biofilm without treatment ( A ) and after treatment with octenidine hydrochloride ( B ).

    Journal: Pathogens

    Article Title: Antibiofilm Effect of Octenidine Hydrochloride on Staphylococcus aureus, MRSA and VRSA

    doi: 10.3390/pathogens3020404

    Figure Lengend Snippet: Confocal microscopy of MRSA (NRS 385) biofilm without treatment ( A ) and after treatment with octenidine hydrochloride ( B ).

    Article Snippet: Following biofilm formation, the effect of OH was tested at 0 (negative control), 2.5 (1.5 µL), 5 (2.5 µL) and 10 (5 µL) mM concentrations with an exposure time of 0, 2, 5 and 10 min. After exposure to OH for the specified time, the wells were washed three times with 200 µL of sterile PBS, dried at room temperature and finally stained with 1% crystal violet for 15 min. After rinsing three times with sterile distilled water and subsequent destaining with 95% ethanol, the absorbance of the adherent biofilm was measured at 570 nm in a microplate reader (Model 550, Bio-Rad, Hercules, CA, USA).

    Techniques: Confocal Microscopy

    Inactivation of S . aureus (ATCC 35556), VRSA (VRS 8) and MRSA (NRS 123) biofilm on stainless steel by octenidine hydrochloride. Duplicate samples were used for each treatment, and the experiment was replicated three times. Data are represented as the mean ± SEM (Standard Error of Mean).

    Journal: Pathogens

    Article Title: Antibiofilm Effect of Octenidine Hydrochloride on Staphylococcus aureus, MRSA and VRSA

    doi: 10.3390/pathogens3020404

    Figure Lengend Snippet: Inactivation of S . aureus (ATCC 35556), VRSA (VRS 8) and MRSA (NRS 123) biofilm on stainless steel by octenidine hydrochloride. Duplicate samples were used for each treatment, and the experiment was replicated three times. Data are represented as the mean ± SEM (Standard Error of Mean).

    Article Snippet: Following biofilm formation, the effect of OH was tested at 0 (negative control), 2.5 (1.5 µL), 5 (2.5 µL) and 10 (5 µL) mM concentrations with an exposure time of 0, 2, 5 and 10 min. After exposure to OH for the specified time, the wells were washed three times with 200 µL of sterile PBS, dried at room temperature and finally stained with 1% crystal violet for 15 min. After rinsing three times with sterile distilled water and subsequent destaining with 95% ethanol, the absorbance of the adherent biofilm was measured at 570 nm in a microplate reader (Model 550, Bio-Rad, Hercules, CA, USA).

    Techniques:

    Inactivation of S . aureus (ATCC 35556), VRSA (VRS 8) and MRSA (NRS 123) biofilm on urinary catheters by octenidine hydrochloride. Duplicate samples were used for each treatment, and the experiment was replicated three times. Data are represented as the mean ± SEM (Standard Error of Mean).

    Journal: Pathogens

    Article Title: Antibiofilm Effect of Octenidine Hydrochloride on Staphylococcus aureus, MRSA and VRSA

    doi: 10.3390/pathogens3020404

    Figure Lengend Snippet: Inactivation of S . aureus (ATCC 35556), VRSA (VRS 8) and MRSA (NRS 123) biofilm on urinary catheters by octenidine hydrochloride. Duplicate samples were used for each treatment, and the experiment was replicated three times. Data are represented as the mean ± SEM (Standard Error of Mean).

    Article Snippet: Following biofilm formation, the effect of OH was tested at 0 (negative control), 2.5 (1.5 µL), 5 (2.5 µL) and 10 (5 µL) mM concentrations with an exposure time of 0, 2, 5 and 10 min. After exposure to OH for the specified time, the wells were washed three times with 200 µL of sterile PBS, dried at room temperature and finally stained with 1% crystal violet for 15 min. After rinsing three times with sterile distilled water and subsequent destaining with 95% ethanol, the absorbance of the adherent biofilm was measured at 570 nm in a microplate reader (Model 550, Bio-Rad, Hercules, CA, USA).

    Techniques:

    Inactivation of S . aureus (ATCC 35556), VRSA (VRS 8) and MRSA (NRS 123) biofilm on polystyrene by octenidine hydrochloride. Duplicate samples were used for each treatment, and the experiment was replicated three times. Data are represented as the mean ± SEM(Standard Error of Mean).

    Journal: Pathogens

    Article Title: Antibiofilm Effect of Octenidine Hydrochloride on Staphylococcus aureus, MRSA and VRSA

    doi: 10.3390/pathogens3020404

    Figure Lengend Snippet: Inactivation of S . aureus (ATCC 35556), VRSA (VRS 8) and MRSA (NRS 123) biofilm on polystyrene by octenidine hydrochloride. Duplicate samples were used for each treatment, and the experiment was replicated three times. Data are represented as the mean ± SEM(Standard Error of Mean).

    Article Snippet: Following biofilm formation, the effect of OH was tested at 0 (negative control), 2.5 (1.5 µL), 5 (2.5 µL) and 10 (5 µL) mM concentrations with an exposure time of 0, 2, 5 and 10 min. After exposure to OH for the specified time, the wells were washed three times with 200 µL of sterile PBS, dried at room temperature and finally stained with 1% crystal violet for 15 min. After rinsing three times with sterile distilled water and subsequent destaining with 95% ethanol, the absorbance of the adherent biofilm was measured at 570 nm in a microplate reader (Model 550, Bio-Rad, Hercules, CA, USA).

    Techniques:

    Inhibition of S . aureus (ATCC 35556), vancomycin-resistant S . aureus (VRSA) (VRS 8) and MRSA (NRS 123) biofilm formation on polystyrene by octenidine hydrochloride (OH). Duplicate samples were used for each treatment, and the experiment was replicated three times. Data are represented as the mean ± SEM.

    Journal: Pathogens

    Article Title: Antibiofilm Effect of Octenidine Hydrochloride on Staphylococcus aureus, MRSA and VRSA

    doi: 10.3390/pathogens3020404

    Figure Lengend Snippet: Inhibition of S . aureus (ATCC 35556), vancomycin-resistant S . aureus (VRSA) (VRS 8) and MRSA (NRS 123) biofilm formation on polystyrene by octenidine hydrochloride (OH). Duplicate samples were used for each treatment, and the experiment was replicated three times. Data are represented as the mean ± SEM.

    Article Snippet: Following biofilm formation, the effect of OH was tested at 0 (negative control), 2.5 (1.5 µL), 5 (2.5 µL) and 10 (5 µL) mM concentrations with an exposure time of 0, 2, 5 and 10 min. After exposure to OH for the specified time, the wells were washed three times with 200 µL of sterile PBS, dried at room temperature and finally stained with 1% crystal violet for 15 min. After rinsing three times with sterile distilled water and subsequent destaining with 95% ethanol, the absorbance of the adherent biofilm was measured at 570 nm in a microplate reader (Model 550, Bio-Rad, Hercules, CA, USA).

    Techniques: Inhibition

    Inhibition of S . aureus (ATCC 35556), VRSA (VRS 8) and MRSA (NRS 123) biofilm on stainless steel by octenidine hydrochloride. Duplicate samples were used for each treatment, and the experiment was replicated three times. Data are represented as the mean ± SEM (Standard Error of Mean).

    Journal: Pathogens

    Article Title: Antibiofilm Effect of Octenidine Hydrochloride on Staphylococcus aureus, MRSA and VRSA

    doi: 10.3390/pathogens3020404

    Figure Lengend Snippet: Inhibition of S . aureus (ATCC 35556), VRSA (VRS 8) and MRSA (NRS 123) biofilm on stainless steel by octenidine hydrochloride. Duplicate samples were used for each treatment, and the experiment was replicated three times. Data are represented as the mean ± SEM (Standard Error of Mean).

    Article Snippet: Following biofilm formation, the effect of OH was tested at 0 (negative control), 2.5 (1.5 µL), 5 (2.5 µL) and 10 (5 µL) mM concentrations with an exposure time of 0, 2, 5 and 10 min. After exposure to OH for the specified time, the wells were washed three times with 200 µL of sterile PBS, dried at room temperature and finally stained with 1% crystal violet for 15 min. After rinsing three times with sterile distilled water and subsequent destaining with 95% ethanol, the absorbance of the adherent biofilm was measured at 570 nm in a microplate reader (Model 550, Bio-Rad, Hercules, CA, USA).

    Techniques: Inhibition

    Antibiofilm effects of water solution of Houttuynia cordata poultice ethanol extract (wHCP) on 6 h biofilm formation by S. mutans MT8148 (a). Antibiofilm effects of water solution of Houttuynia cordata poultice ethanol extract (wHCP) on 24 h biofilm formations by Candida albicans CAD1 (b). Distilled H 2 O (1%, 5%, or 10%) was used as a negative control. ∗ Significant differences between the indicated groups at P

    Journal: BioMed Research International

    Article Title: Preventive Effects of Houttuynia cordata Extract for Oral Infectious Diseases

    doi: 10.1155/2016/2581876

    Figure Lengend Snippet: Antibiofilm effects of water solution of Houttuynia cordata poultice ethanol extract (wHCP) on 6 h biofilm formation by S. mutans MT8148 (a). Antibiofilm effects of water solution of Houttuynia cordata poultice ethanol extract (wHCP) on 24 h biofilm formations by Candida albicans CAD1 (b). Distilled H 2 O (1%, 5%, or 10%) was used as a negative control. ∗ Significant differences between the indicated groups at P

    Article Snippet: After drying, the stained biofilm was extracted from the well by adding 150 μ L of ethanol, and the absorbance of the extract from stained biofilm was measured at 595 nm using a microplate reader (model 680; Bio-Rad Laboratories, Hercules, CA, USA).

    Techniques: Negative Control

    Antibiofilm effects of water solution of Houttuynia cordata poultice ethanol extract (wHCP) on 24 h biofilm formations by MRSA T31 (a). Antibiofilm effects of water solution of Houttuynia cordata poultice ethanol extract (wHCP) on 24 h biofilm formations by F. nucleatum (b). Distilled H 2 O (1%, 5%, or 10%) was used as a negative control. ∗ Significant differences between the indicated groups at P

    Journal: BioMed Research International

    Article Title: Preventive Effects of Houttuynia cordata Extract for Oral Infectious Diseases

    doi: 10.1155/2016/2581876

    Figure Lengend Snippet: Antibiofilm effects of water solution of Houttuynia cordata poultice ethanol extract (wHCP) on 24 h biofilm formations by MRSA T31 (a). Antibiofilm effects of water solution of Houttuynia cordata poultice ethanol extract (wHCP) on 24 h biofilm formations by F. nucleatum (b). Distilled H 2 O (1%, 5%, or 10%) was used as a negative control. ∗ Significant differences between the indicated groups at P

    Article Snippet: After drying, the stained biofilm was extracted from the well by adding 150 μ L of ethanol, and the absorbance of the extract from stained biofilm was measured at 595 nm using a microplate reader (model 680; Bio-Rad Laboratories, Hercules, CA, USA).

    Techniques: Negative Control

    Scanning electron microscopy of 4 Trichosporon spp. strains grown on catheter surfaces. A and B : Low biofilm producer T. asahii 05-001 (CV-A 570 = 0.287); C and D : Medium biofilm producer T. asahii 18-001 (CV-A 570 = 1.557); E and F : High biofilm producer T. asahii 07-001A (CV-A 570 = 3.337); and G and H : High biofilm producer T. asteroides 13-001 (CV-A 570 = 2.755).

    Journal: PLoS ONE

    Article Title: Multiple Species of Trichosporon Produce Biofilms Highly Resistant to Triazoles and Amphotericin B

    doi: 10.1371/journal.pone.0109553

    Figure Lengend Snippet: Scanning electron microscopy of 4 Trichosporon spp. strains grown on catheter surfaces. A and B : Low biofilm producer T. asahii 05-001 (CV-A 570 = 0.287); C and D : Medium biofilm producer T. asahii 18-001 (CV-A 570 = 1.557); E and F : High biofilm producer T. asahii 07-001A (CV-A 570 = 3.337); and G and H : High biofilm producer T. asteroides 13-001 (CV-A 570 = 2.755).

    Article Snippet: To destain the biofilms, 200 µl 95% ethanol was added, and the plate was incubated for 45 min. One hundred μl of the solution were transferred to another microplate, and the absorbance was read using a Microplate Reader 680 (BIO RAD, Hercules, USA) at 570 nm.

    Techniques: Electron Microscopy

    Median-joining genotypes network of T. asahii, T. faecale and T. coremiiforme based on IGS1 rDNA sequences related to biofilm quantitation. Dashed square groups the 12 different genotypes (G1 to G12) of T. asahii . Dashed circle groups the 3 different genotypes of T. faecale . Circumference sizes are proportional to the genotype frequencies. Black dots (mv = median vectors) are hypothetical missing intermediates.

    Journal: PLoS ONE

    Article Title: Multiple Species of Trichosporon Produce Biofilms Highly Resistant to Triazoles and Amphotericin B

    doi: 10.1371/journal.pone.0109553

    Figure Lengend Snippet: Median-joining genotypes network of T. asahii, T. faecale and T. coremiiforme based on IGS1 rDNA sequences related to biofilm quantitation. Dashed square groups the 12 different genotypes (G1 to G12) of T. asahii . Dashed circle groups the 3 different genotypes of T. faecale . Circumference sizes are proportional to the genotype frequencies. Black dots (mv = median vectors) are hypothetical missing intermediates.

    Article Snippet: To destain the biofilms, 200 µl 95% ethanol was added, and the plate was incubated for 45 min. One hundred μl of the solution were transferred to another microplate, and the absorbance was read using a Microplate Reader 680 (BIO RAD, Hercules, USA) at 570 nm.

    Techniques: Quantitation Assay

    Inter and intra species variation in the biofilm production of 54 Trichosporon spp. clinical isolates and 7 reference strains. A- 19 isolates from blood identified as T. asahii, T. asteroides, T. coremiiforme and T. dermatis . B- 20 isolates from urine identified as T. asahii . C- 15 isolates from superficial mycosis/skin colonization identified as T. asahii, T. dermatis, T. faecale and T. inkin . D- 7 reference strains from CBS obtained from different sources.

    Journal: PLoS ONE

    Article Title: Multiple Species of Trichosporon Produce Biofilms Highly Resistant to Triazoles and Amphotericin B

    doi: 10.1371/journal.pone.0109553

    Figure Lengend Snippet: Inter and intra species variation in the biofilm production of 54 Trichosporon spp. clinical isolates and 7 reference strains. A- 19 isolates from blood identified as T. asahii, T. asteroides, T. coremiiforme and T. dermatis . B- 20 isolates from urine identified as T. asahii . C- 15 isolates from superficial mycosis/skin colonization identified as T. asahii, T. dermatis, T. faecale and T. inkin . D- 7 reference strains from CBS obtained from different sources.

    Article Snippet: To destain the biofilms, 200 µl 95% ethanol was added, and the plate was incubated for 45 min. One hundred μl of the solution were transferred to another microplate, and the absorbance was read using a Microplate Reader 680 (BIO RAD, Hercules, USA) at 570 nm.

    Techniques:

    Effect of hLF1-11 on biofilm architecture of C . albicans strains. Inverted microscope images (400× magnification) of (a) C . albicans SC5314 and three clinical isolates, (b) CA688, (c) CA22, and (d) CA37, untreated and treated with 44mg/L, 88mg/L and 176mg/L hLF1-11. C . albicans strains were co-incubated with the different hLF1-11 concentrations at 37°C for 24h.

    Journal: PLoS ONE

    Article Title: Inhibition of Candida albicans Biofilm Formation by the Synthetic Lactoferricin Derived Peptide hLF1-11

    doi: 10.1371/journal.pone.0167470

    Figure Lengend Snippet: Effect of hLF1-11 on biofilm architecture of C . albicans strains. Inverted microscope images (400× magnification) of (a) C . albicans SC5314 and three clinical isolates, (b) CA688, (c) CA22, and (d) CA37, untreated and treated with 44mg/L, 88mg/L and 176mg/L hLF1-11. C . albicans strains were co-incubated with the different hLF1-11 concentrations at 37°C for 24h.

    Article Snippet: Biofilm cellular density Biofilm cellular density formed in the presence or absence of the peptide was determined by measuring the optical density at 490 nm (ODλ 490nm ) using an automated plate reader (Model 550 Microplate Reader Bio-Rad, Milan Italy).

    Techniques: Inverted Microscopy, Incubation

    Effect of hLF1-11 on biofilm formation by four representative C . albicans strains . C . albicans cells (1x10 6 cells/ml) were co-incubated with various concentrations of hLF1-11 for 24h at 37°C. After incubation, the antibiofilm activity of the peptide was assessed in terms of (a) biofilm cellular density reduction, (b) metabolic activity by the XTT assay, and c reduction of sessile cell viability. Data are expressed as the mean of three independent experiments ± SEM. SC5314 open bars, CA688 closed bars, CA22 dotted bars, CA37 diagonally hatched bars * P ≤0.05, ** P

    Journal: PLoS ONE

    Article Title: Inhibition of Candida albicans Biofilm Formation by the Synthetic Lactoferricin Derived Peptide hLF1-11

    doi: 10.1371/journal.pone.0167470

    Figure Lengend Snippet: Effect of hLF1-11 on biofilm formation by four representative C . albicans strains . C . albicans cells (1x10 6 cells/ml) were co-incubated with various concentrations of hLF1-11 for 24h at 37°C. After incubation, the antibiofilm activity of the peptide was assessed in terms of (a) biofilm cellular density reduction, (b) metabolic activity by the XTT assay, and c reduction of sessile cell viability. Data are expressed as the mean of three independent experiments ± SEM. SC5314 open bars, CA688 closed bars, CA22 dotted bars, CA37 diagonally hatched bars * P ≤0.05, ** P

    Article Snippet: Biofilm cellular density Biofilm cellular density formed in the presence or absence of the peptide was determined by measuring the optical density at 490 nm (ODλ 490nm ) using an automated plate reader (Model 550 Microplate Reader Bio-Rad, Milan Italy).

    Techniques: Incubation, Activity Assay, XTT Assay

    Effect of hLF1-11 on pre-adhered C . albicans cells. C . albicans SC5314 cells were allowed to adhere for different times (1.5h, 6h and 24h) and then various concentrations of hLF1-11 were added and incubated for 24h at 37°C. Following incubation, the antibiofilm activity of the peptide was assessed in terms of (a) biofilm cellular density reduction, (b) metabolic activity by the XTT assay, and (c) reduction of sessile cell viability. Data are expressed as the mean of three independent experiments ± SEM. 1.5h closed bars, 6h open bars, 24h diagonally hatched bars * P ≤0.05, ** P

    Journal: PLoS ONE

    Article Title: Inhibition of Candida albicans Biofilm Formation by the Synthetic Lactoferricin Derived Peptide hLF1-11

    doi: 10.1371/journal.pone.0167470

    Figure Lengend Snippet: Effect of hLF1-11 on pre-adhered C . albicans cells. C . albicans SC5314 cells were allowed to adhere for different times (1.5h, 6h and 24h) and then various concentrations of hLF1-11 were added and incubated for 24h at 37°C. Following incubation, the antibiofilm activity of the peptide was assessed in terms of (a) biofilm cellular density reduction, (b) metabolic activity by the XTT assay, and (c) reduction of sessile cell viability. Data are expressed as the mean of three independent experiments ± SEM. 1.5h closed bars, 6h open bars, 24h diagonally hatched bars * P ≤0.05, ** P

    Article Snippet: Biofilm cellular density Biofilm cellular density formed in the presence or absence of the peptide was determined by measuring the optical density at 490 nm (ODλ 490nm ) using an automated plate reader (Model 550 Microplate Reader Bio-Rad, Milan Italy).

    Techniques: Incubation, Activity Assay, XTT Assay

    Kinetics of hLF1-11 activity on biofilm formation by four representative C . albicans strains. C . albicans SC5314 cells (1x10 6 cells/ml) were co-incubated with hLF1-11 (88mg/L) for different time periods (1.5, 3, 6 and 24h) at 37°C. Following incubation, the antibiofilm activity of the peptide was assessed in terms of (a) biofilm cellular density reduction, (b) metabolic activity by the XTT assay, and (c) reduction of sessile cell viability. Data are expressed as the mean of three independent experiments ± SEM. hLF1-11-treated sample (square symbol), untreated sample (triangle symbol) ** P

    Journal: PLoS ONE

    Article Title: Inhibition of Candida albicans Biofilm Formation by the Synthetic Lactoferricin Derived Peptide hLF1-11

    doi: 10.1371/journal.pone.0167470

    Figure Lengend Snippet: Kinetics of hLF1-11 activity on biofilm formation by four representative C . albicans strains. C . albicans SC5314 cells (1x10 6 cells/ml) were co-incubated with hLF1-11 (88mg/L) for different time periods (1.5, 3, 6 and 24h) at 37°C. Following incubation, the antibiofilm activity of the peptide was assessed in terms of (a) biofilm cellular density reduction, (b) metabolic activity by the XTT assay, and (c) reduction of sessile cell viability. Data are expressed as the mean of three independent experiments ± SEM. hLF1-11-treated sample (square symbol), untreated sample (triangle symbol) ** P

    Article Snippet: Biofilm cellular density Biofilm cellular density formed in the presence or absence of the peptide was determined by measuring the optical density at 490 nm (ODλ 490nm ) using an automated plate reader (Model 550 Microplate Reader Bio-Rad, Milan Italy).

    Techniques: Activity Assay, Incubation, XTT Assay

    Quantitative real time RT-PCR analysis of C . albicans biofilm-related genes. C . albicans SC5314 biofilms were treated with 88mg/L of hLF1-11 in 24-well plates for 24h at 37°C and the expression of the target genes was determined qRT-PCR. Level of gene expression (closed column) is presented as fold change relative to the control group (untreated biofilms, open column). ACT1 expression was used for normalization. Assays were performed in triplicate and bars represent means ± S.E.M from three independent experiments. ** P

    Journal: PLoS ONE

    Article Title: Inhibition of Candida albicans Biofilm Formation by the Synthetic Lactoferricin Derived Peptide hLF1-11

    doi: 10.1371/journal.pone.0167470

    Figure Lengend Snippet: Quantitative real time RT-PCR analysis of C . albicans biofilm-related genes. C . albicans SC5314 biofilms were treated with 88mg/L of hLF1-11 in 24-well plates for 24h at 37°C and the expression of the target genes was determined qRT-PCR. Level of gene expression (closed column) is presented as fold change relative to the control group (untreated biofilms, open column). ACT1 expression was used for normalization. Assays were performed in triplicate and bars represent means ± S.E.M from three independent experiments. ** P

    Article Snippet: Biofilm cellular density Biofilm cellular density formed in the presence or absence of the peptide was determined by measuring the optical density at 490 nm (ODλ 490nm ) using an automated plate reader (Model 550 Microplate Reader Bio-Rad, Milan Italy).

    Techniques: Quantitative RT-PCR, Expressing