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Agilent technologies bioanalyzer rna nano 6000 kit
<t>Bioanalyzer</t> profiles of the large <t>RNA</t> fractions isolated from donors with the lowest and highest blood RNA level. The large RNA fractions (200 ng) from the low RNA level sample 180 (6.7 μg RNA/ml blood) and the high RNA level sample 162 (22.7 μg RNA/ml blood) were separated using the Bioanalyzer RNA <t>Nano</t> 6000 Kit. Shown are positions of: the (18S) and (28S) ribosomal RNA; the 600 nt globin region (G); and Bioanalyzer marker (M).
Bioanalyzer Rna Nano 6000 Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bioanalyzer rna nano 6000 kit/product/Agilent technologies
Average 90 stars, based on 1 article reviews
Price from $9.99 to $1999.99
bioanalyzer rna nano 6000 kit - by Bioz Stars, 2020-09
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1) Product Images from "Inter-Individual Differences in RNA Levels in Human Peripheral Blood"

Article Title: Inter-Individual Differences in RNA Levels in Human Peripheral Blood

Journal: PLoS ONE

doi: 10.1371/journal.pone.0148260

Bioanalyzer profiles of the large RNA fractions isolated from donors with the lowest and highest blood RNA level. The large RNA fractions (200 ng) from the low RNA level sample 180 (6.7 μg RNA/ml blood) and the high RNA level sample 162 (22.7 μg RNA/ml blood) were separated using the Bioanalyzer RNA Nano 6000 Kit. Shown are positions of: the (18S) and (28S) ribosomal RNA; the 600 nt globin region (G); and Bioanalyzer marker (M).
Figure Legend Snippet: Bioanalyzer profiles of the large RNA fractions isolated from donors with the lowest and highest blood RNA level. The large RNA fractions (200 ng) from the low RNA level sample 180 (6.7 μg RNA/ml blood) and the high RNA level sample 162 (22.7 μg RNA/ml blood) were separated using the Bioanalyzer RNA Nano 6000 Kit. Shown are positions of: the (18S) and (28S) ribosomal RNA; the 600 nt globin region (G); and Bioanalyzer marker (M).

Techniques Used: Isolation, Marker

Characteristics of RNA isolated from healthy donors. a) Proportion of the large and small RNA fractions in relation to increasing quantities of total RNA in human peripheral blood. Samples of RNA from the 35 individuals are sequentially ranked in accord with the increasing amount of total RNA in the sample. For each sample, the amounts of the large RNA fraction (inverted triangle) and small RNA fraction (square) are depicted. b) The large RNA and small RNA fractions are expressed as a percentage of the total RNA in the sample. c) Bioanalyzer RIN values of the large RNA fractions from 35 samples.
Figure Legend Snippet: Characteristics of RNA isolated from healthy donors. a) Proportion of the large and small RNA fractions in relation to increasing quantities of total RNA in human peripheral blood. Samples of RNA from the 35 individuals are sequentially ranked in accord with the increasing amount of total RNA in the sample. For each sample, the amounts of the large RNA fraction (inverted triangle) and small RNA fraction (square) are depicted. b) The large RNA and small RNA fractions are expressed as a percentage of the total RNA in the sample. c) Bioanalyzer RIN values of the large RNA fractions from 35 samples.

Techniques Used: Isolation

Related Articles

Isolation:

Article Title: Inter-Individual Differences in RNA Levels in Human Peripheral Blood
Article Snippet: .. The isolated RNA samples were analyzed with the Agilent Bioanalyzer RNA Nano 6000 Kit (Agilent Technologies, Inc., Santa Clara, CA). .. Genomic DNA was isolated from the frozen phenolic fraction by extraction with guanidine thiocyanate at alkaline pH as described in the RNAzol® BD manufacturer’s brochure.

RNA HS Assay:

Article Title: Multivalent role of human TFIID in recruiting elongation components at the promoter proximal region for transcriptional control
Article Snippet: .. The RNA quantity was checked using Qubit RNA HS assay kit (Thermo Fisher Scientific) and RNA quality was checked with Agilent Bioanalyzer RNA Nano 6000 Kit (Agilent Technologies). ..

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  • 90
    Agilent technologies bioanalyzer rna nano 6000 kit
    <t>Bioanalyzer</t> profiles of the large <t>RNA</t> fractions isolated from donors with the lowest and highest blood RNA level. The large RNA fractions (200 ng) from the low RNA level sample 180 (6.7 μg RNA/ml blood) and the high RNA level sample 162 (22.7 μg RNA/ml blood) were separated using the Bioanalyzer RNA <t>Nano</t> 6000 Kit. Shown are positions of: the (18S) and (28S) ribosomal RNA; the 600 nt globin region (G); and Bioanalyzer marker (M).
    Bioanalyzer Rna Nano 6000 Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bioanalyzer rna nano 6000 kit/product/Agilent technologies
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bioanalyzer rna nano 6000 kit - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    91
    Agilent technologies rna 6000 nano chip kit
    Decrease in RNA integrity after time-dependent thermal degradation of total RNA isolated from renal cell lines A-498 and HEK-293, and the kidney cancer tissue pool. The RNA samples were incubated at 80 °C in Eppendorf tubes for different time points and subsequently transferred to an ice-bath to stop the degradation and stored at -80 °C until analysis. The RIN values at the time points were for the A-498 cells: 10.0, 9.0, 8.2, 7.1, 6.1, 4.4, 3.3, and 2.7; for the HEK-293 cells: 9.7, 8.3, 7.1, 5.8, 4.9, 3.7, 2.8, and 1.9 (the last sample was excluded in further analysis); and for the tissue pool: 8.2, 7.9, 7.0, 6.6, 5.8, 4.6, 3.6, and 3.0. The gel like view of total RNA samples after heat degradation analyzed with the total Agilent <t>RNA</t> 6000 Nano Chip Kit on the Agilent 2100 bioanalyzer is shown in Figure S1 .
    Rna 6000 Nano Chip Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna 6000 nano chip kit/product/Agilent technologies
    Average 91 stars, based on 46 article reviews
    Price from $9.99 to $1999.99
    rna 6000 nano chip kit - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    90
    Agilent technologies rna 6000 nano assay kits
    Electropherograms of total RNA from cassava obtained using our method showing 18S and 25S rRNA regions with RNA concentrations and RIN values; (A) to (C) correspond with RNA from leaves and storage roots, and (A) and (B) correspond with RNA from different stages of plant development (young and mature leaves). RNA were visualized in denaturing agarose gels stained with SYBR safe. RNA were analyzed using Agilent <t>RNA</t> 6000 <t>Nano</t> Assays in a 2100 Bioanalyzer (Agilent Technologies) and were then used for RNA sequencing.
    Rna 6000 Nano Assay Kits, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1511 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna 6000 nano assay kits/product/Agilent technologies
    Average 90 stars, based on 1511 article reviews
    Price from $9.99 to $1999.99
    rna 6000 nano assay kits - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

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    Bioanalyzer profiles of the large RNA fractions isolated from donors with the lowest and highest blood RNA level. The large RNA fractions (200 ng) from the low RNA level sample 180 (6.7 μg RNA/ml blood) and the high RNA level sample 162 (22.7 μg RNA/ml blood) were separated using the Bioanalyzer RNA Nano 6000 Kit. Shown are positions of: the (18S) and (28S) ribosomal RNA; the 600 nt globin region (G); and Bioanalyzer marker (M).

    Journal: PLoS ONE

    Article Title: Inter-Individual Differences in RNA Levels in Human Peripheral Blood

    doi: 10.1371/journal.pone.0148260

    Figure Lengend Snippet: Bioanalyzer profiles of the large RNA fractions isolated from donors with the lowest and highest blood RNA level. The large RNA fractions (200 ng) from the low RNA level sample 180 (6.7 μg RNA/ml blood) and the high RNA level sample 162 (22.7 μg RNA/ml blood) were separated using the Bioanalyzer RNA Nano 6000 Kit. Shown are positions of: the (18S) and (28S) ribosomal RNA; the 600 nt globin region (G); and Bioanalyzer marker (M).

    Article Snippet: The isolated RNA samples were analyzed with the Agilent Bioanalyzer RNA Nano 6000 Kit (Agilent Technologies, Inc., Santa Clara, CA).

    Techniques: Isolation, Marker

    Characteristics of RNA isolated from healthy donors. a) Proportion of the large and small RNA fractions in relation to increasing quantities of total RNA in human peripheral blood. Samples of RNA from the 35 individuals are sequentially ranked in accord with the increasing amount of total RNA in the sample. For each sample, the amounts of the large RNA fraction (inverted triangle) and small RNA fraction (square) are depicted. b) The large RNA and small RNA fractions are expressed as a percentage of the total RNA in the sample. c) Bioanalyzer RIN values of the large RNA fractions from 35 samples.

    Journal: PLoS ONE

    Article Title: Inter-Individual Differences in RNA Levels in Human Peripheral Blood

    doi: 10.1371/journal.pone.0148260

    Figure Lengend Snippet: Characteristics of RNA isolated from healthy donors. a) Proportion of the large and small RNA fractions in relation to increasing quantities of total RNA in human peripheral blood. Samples of RNA from the 35 individuals are sequentially ranked in accord with the increasing amount of total RNA in the sample. For each sample, the amounts of the large RNA fraction (inverted triangle) and small RNA fraction (square) are depicted. b) The large RNA and small RNA fractions are expressed as a percentage of the total RNA in the sample. c) Bioanalyzer RIN values of the large RNA fractions from 35 samples.

    Article Snippet: The isolated RNA samples were analyzed with the Agilent Bioanalyzer RNA Nano 6000 Kit (Agilent Technologies, Inc., Santa Clara, CA).

    Techniques: Isolation

    Decrease in RNA integrity after time-dependent thermal degradation of total RNA isolated from renal cell lines A-498 and HEK-293, and the kidney cancer tissue pool. The RNA samples were incubated at 80 °C in Eppendorf tubes for different time points and subsequently transferred to an ice-bath to stop the degradation and stored at -80 °C until analysis. The RIN values at the time points were for the A-498 cells: 10.0, 9.0, 8.2, 7.1, 6.1, 4.4, 3.3, and 2.7; for the HEK-293 cells: 9.7, 8.3, 7.1, 5.8, 4.9, 3.7, 2.8, and 1.9 (the last sample was excluded in further analysis); and for the tissue pool: 8.2, 7.9, 7.0, 6.6, 5.8, 4.6, 3.6, and 3.0. The gel like view of total RNA samples after heat degradation analyzed with the total Agilent RNA 6000 Nano Chip Kit on the Agilent 2100 bioanalyzer is shown in Figure S1 .

    Journal: Theranostics

    Article Title: Instability of circular RNAs in clinical tissue samples impairs their reliable expression analysis using RT-qPCR: from the myth of their advantage as biomarkers to reality

    doi: 10.7150/thno.46341

    Figure Lengend Snippet: Decrease in RNA integrity after time-dependent thermal degradation of total RNA isolated from renal cell lines A-498 and HEK-293, and the kidney cancer tissue pool. The RNA samples were incubated at 80 °C in Eppendorf tubes for different time points and subsequently transferred to an ice-bath to stop the degradation and stored at -80 °C until analysis. The RIN values at the time points were for the A-498 cells: 10.0, 9.0, 8.2, 7.1, 6.1, 4.4, 3.3, and 2.7; for the HEK-293 cells: 9.7, 8.3, 7.1, 5.8, 4.9, 3.7, 2.8, and 1.9 (the last sample was excluded in further analysis); and for the tissue pool: 8.2, 7.9, 7.0, 6.6, 5.8, 4.6, 3.6, and 3.0. The gel like view of total RNA samples after heat degradation analyzed with the total Agilent RNA 6000 Nano Chip Kit on the Agilent 2100 bioanalyzer is shown in Figure S1 .

    Article Snippet: The RIN was assessed on a Bioanalyzer 2100 with the Agilent RNA 6000 Nano Chip Kit (Agilent Technologies, Santa Clara, CA, USA; Cat. No. 5067-1511).

    Techniques: Isolation, Incubation, Chromatin Immunoprecipitation

    Electropherograms of total RNA from cassava obtained using our method showing 18S and 25S rRNA regions with RNA concentrations and RIN values; (A) to (C) correspond with RNA from leaves and storage roots, and (A) and (B) correspond with RNA from different stages of plant development (young and mature leaves). RNA were visualized in denaturing agarose gels stained with SYBR safe. RNA were analyzed using Agilent RNA 6000 Nano Assays in a 2100 Bioanalyzer (Agilent Technologies) and were then used for RNA sequencing.

    Journal: FEBS Open Bio

    Article Title: An optimized isolation protocol yields high‐quality RNA from cassava tissues (Manihot esculenta Crantz)

    doi: 10.1002/2211-5463.12561

    Figure Lengend Snippet: Electropherograms of total RNA from cassava obtained using our method showing 18S and 25S rRNA regions with RNA concentrations and RIN values; (A) to (C) correspond with RNA from leaves and storage roots, and (A) and (B) correspond with RNA from different stages of plant development (young and mature leaves). RNA were visualized in denaturing agarose gels stained with SYBR safe. RNA were analyzed using Agilent RNA 6000 Nano Assays in a 2100 Bioanalyzer (Agilent Technologies) and were then used for RNA sequencing.

    Article Snippet: Thus, we evaluated RNA integrity according to RIN using a 2100 Bioanalyzer instrument with Agilent RNA 6000 Nano Assay kits (Agilent Technologies).

    Techniques: Staining, RNA Sequencing Assay