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Agilent technologies bioanalyzer rna nano 6000 kit
<t>Bioanalyzer</t> profiles of the large <t>RNA</t> fractions isolated from donors with the lowest and highest blood RNA level. The large RNA fractions (200 ng) from the low RNA level sample 180 (6.7 μg RNA/ml blood) and the high RNA level sample 162 (22.7 μg RNA/ml blood) were separated using the Bioanalyzer RNA Nano 6000 Kit. Shown are positions of: the (18S) and (28S) ribosomal RNA; the 600 nt globin region (G); and Bioanalyzer marker (M).
Bioanalyzer Rna Nano 6000 Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Inter-Individual Differences in RNA Levels in Human Peripheral Blood"

Article Title: Inter-Individual Differences in RNA Levels in Human Peripheral Blood

Journal: PLoS ONE

doi: 10.1371/journal.pone.0148260

Bioanalyzer profiles of the large RNA fractions isolated from donors with the lowest and highest blood RNA level. The large RNA fractions (200 ng) from the low RNA level sample 180 (6.7 μg RNA/ml blood) and the high RNA level sample 162 (22.7 μg RNA/ml blood) were separated using the Bioanalyzer RNA Nano 6000 Kit. Shown are positions of: the (18S) and (28S) ribosomal RNA; the 600 nt globin region (G); and Bioanalyzer marker (M).
Figure Legend Snippet: Bioanalyzer profiles of the large RNA fractions isolated from donors with the lowest and highest blood RNA level. The large RNA fractions (200 ng) from the low RNA level sample 180 (6.7 μg RNA/ml blood) and the high RNA level sample 162 (22.7 μg RNA/ml blood) were separated using the Bioanalyzer RNA Nano 6000 Kit. Shown are positions of: the (18S) and (28S) ribosomal RNA; the 600 nt globin region (G); and Bioanalyzer marker (M).

Techniques Used: Isolation, Marker

Characteristics of RNA isolated from healthy donors. a) Proportion of the large and small RNA fractions in relation to increasing quantities of total RNA in human peripheral blood. Samples of RNA from the 35 individuals are sequentially ranked in accord with the increasing amount of total RNA in the sample. For each sample, the amounts of the large RNA fraction (inverted triangle) and small RNA fraction (square) are depicted. b) The large RNA and small RNA fractions are expressed as a percentage of the total RNA in the sample. c) Bioanalyzer RIN values of the large RNA fractions from 35 samples.
Figure Legend Snippet: Characteristics of RNA isolated from healthy donors. a) Proportion of the large and small RNA fractions in relation to increasing quantities of total RNA in human peripheral blood. Samples of RNA from the 35 individuals are sequentially ranked in accord with the increasing amount of total RNA in the sample. For each sample, the amounts of the large RNA fraction (inverted triangle) and small RNA fraction (square) are depicted. b) The large RNA and small RNA fractions are expressed as a percentage of the total RNA in the sample. c) Bioanalyzer RIN values of the large RNA fractions from 35 samples.

Techniques Used: Isolation

Related Articles

DNA Extraction:

Article Title: Inter-Individual Differences in RNA Levels in Human Peripheral Blood
Article Snippet: Paragraph title: RNA and DNA isolation ... The isolated RNA samples were analyzed with the Agilent Bioanalyzer RNA Nano 6000 Kit (Agilent Technologies, Inc., Santa Clara, CA).

Isolation:

Article Title: Inter-Individual Differences in RNA Levels in Human Peripheral Blood
Article Snippet: Total RNA content in the peripheral blood was calculated as the sum of the large RNA and small RNA fractions. .. The isolated RNA samples were analyzed with the Agilent Bioanalyzer RNA Nano 6000 Kit (Agilent Technologies, Inc., Santa Clara, CA). .. Genomic DNA was isolated from the frozen phenolic fraction by extraction with guanidine thiocyanate at alkaline pH as described in the RNAzol® BD manufacturer’s brochure.

RNA HS Assay:

Article Title: Multivalent role of human TFIID in recruiting elongation components at the promoter proximal region for transcriptional control
Article Snippet: RNA-Seq analyses were performed following the steps as mentioned below. .. The RNA quantity was checked using Qubit RNA HS assay kit (Thermo Fisher Scientific) and RNA quality was checked with Agilent Bioanalyzer RNA Nano 6000 Kit (Agilent Technologies). .. The samples with > 7 RNA integrity number (RIN) was taken further for library preparation.

Concentration Assay:

Article Title: Inter-Individual Differences in RNA Levels in Human Peripheral Blood
Article Snippet: RNA concentration was determined based on optical density (OD) at A260 nm, assuming that 1 OD of RNA dissolved in water corresponds to 40 μg RNA/ml, and 1 OD of RNA dissolved in FORMAzol corresponds to 44.9 μg RNA/ml. .. The isolated RNA samples were analyzed with the Agilent Bioanalyzer RNA Nano 6000 Kit (Agilent Technologies, Inc., Santa Clara, CA).

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    Agilent technologies bioanalyzer rna nano 6000 kit
    <t>Bioanalyzer</t> profiles of the large <t>RNA</t> fractions isolated from donors with the lowest and highest blood RNA level. The large RNA fractions (200 ng) from the low RNA level sample 180 (6.7 μg RNA/ml blood) and the high RNA level sample 162 (22.7 μg RNA/ml blood) were separated using the Bioanalyzer RNA Nano 6000 Kit. Shown are positions of: the (18S) and (28S) ribosomal RNA; the 600 nt globin region (G); and Bioanalyzer marker (M).
    Bioanalyzer Rna Nano 6000 Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bioanalyzer rna nano 6000 kit/product/Agilent technologies
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bioanalyzer rna nano 6000 kit - by Bioz Stars, 2019-12
    78/100 stars
      Buy from Supplier

    99
    Agilent technologies bioanalyzer
    Microsampling protocol design and reproducibility. a In terminal blood sampling, at each time point, groups of mice are exsanguinated to obtain 0.5–0.6 mL blood volumes, which are then subject to leukocyte depletion and saponin lysis before TRIzol treatment. Thus, the number of mice increases proportionally with the number of biological replicates and time points in the study design (number of mice per timepoint X number of timepoints; NT). Microsampling involves obtaining sample volumes as low as 20 μL from the same mouse at different time points, thus confining the number of mice to just biological replicates (N) and significantly lowering costs and biological variability. Leukocyte depletion and saponin lysis are also not performed on the low volume samples, thus saving time and manpower. b Heatmap shows pair-wise Pearson correlation coefficients and the inset shows multidimensional scaling to visualize the level of similarity between the P. vinckei microsamples. Microsamples show low degree of variability and are highly reproducible as proved by tight correlations between biological replicates. c High Pearson correlations were observed between normalized gene expression values (shown as logarithm of fragments per kilobase of transcript per million mapped reads) from microsampling (x-axis) and terminal blood sampling (y-axis) methods. d <t>Bioanalyser</t> electrophoregrams of total RNA from Plasmodium vinckei vinckei CY microsamples show that high quality RNA could be extracted consistently from 20 μL microsamples
    Bioanalyzer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 1701 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bioanalyzer/product/Agilent technologies
    Average 99 stars, based on 1701 article reviews
    Price from $9.99 to $1999.99
    bioanalyzer - by Bioz Stars, 2019-12
    99/100 stars
      Buy from Supplier

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    Bioanalyzer profiles of the large RNA fractions isolated from donors with the lowest and highest blood RNA level. The large RNA fractions (200 ng) from the low RNA level sample 180 (6.7 μg RNA/ml blood) and the high RNA level sample 162 (22.7 μg RNA/ml blood) were separated using the Bioanalyzer RNA Nano 6000 Kit. Shown are positions of: the (18S) and (28S) ribosomal RNA; the 600 nt globin region (G); and Bioanalyzer marker (M).

    Journal: PLoS ONE

    Article Title: Inter-Individual Differences in RNA Levels in Human Peripheral Blood

    doi: 10.1371/journal.pone.0148260

    Figure Lengend Snippet: Bioanalyzer profiles of the large RNA fractions isolated from donors with the lowest and highest blood RNA level. The large RNA fractions (200 ng) from the low RNA level sample 180 (6.7 μg RNA/ml blood) and the high RNA level sample 162 (22.7 μg RNA/ml blood) were separated using the Bioanalyzer RNA Nano 6000 Kit. Shown are positions of: the (18S) and (28S) ribosomal RNA; the 600 nt globin region (G); and Bioanalyzer marker (M).

    Article Snippet: The isolated RNA samples were analyzed with the Agilent Bioanalyzer RNA Nano 6000 Kit (Agilent Technologies, Inc., Santa Clara, CA).

    Techniques: Isolation, Marker

    Characteristics of RNA isolated from healthy donors. a) Proportion of the large and small RNA fractions in relation to increasing quantities of total RNA in human peripheral blood. Samples of RNA from the 35 individuals are sequentially ranked in accord with the increasing amount of total RNA in the sample. For each sample, the amounts of the large RNA fraction (inverted triangle) and small RNA fraction (square) are depicted. b) The large RNA and small RNA fractions are expressed as a percentage of the total RNA in the sample. c) Bioanalyzer RIN values of the large RNA fractions from 35 samples.

    Journal: PLoS ONE

    Article Title: Inter-Individual Differences in RNA Levels in Human Peripheral Blood

    doi: 10.1371/journal.pone.0148260

    Figure Lengend Snippet: Characteristics of RNA isolated from healthy donors. a) Proportion of the large and small RNA fractions in relation to increasing quantities of total RNA in human peripheral blood. Samples of RNA from the 35 individuals are sequentially ranked in accord with the increasing amount of total RNA in the sample. For each sample, the amounts of the large RNA fraction (inverted triangle) and small RNA fraction (square) are depicted. b) The large RNA and small RNA fractions are expressed as a percentage of the total RNA in the sample. c) Bioanalyzer RIN values of the large RNA fractions from 35 samples.

    Article Snippet: The isolated RNA samples were analyzed with the Agilent Bioanalyzer RNA Nano 6000 Kit (Agilent Technologies, Inc., Santa Clara, CA).

    Techniques: Isolation

    Microsampling protocol design and reproducibility. a In terminal blood sampling, at each time point, groups of mice are exsanguinated to obtain 0.5–0.6 mL blood volumes, which are then subject to leukocyte depletion and saponin lysis before TRIzol treatment. Thus, the number of mice increases proportionally with the number of biological replicates and time points in the study design (number of mice per timepoint X number of timepoints; NT). Microsampling involves obtaining sample volumes as low as 20 μL from the same mouse at different time points, thus confining the number of mice to just biological replicates (N) and significantly lowering costs and biological variability. Leukocyte depletion and saponin lysis are also not performed on the low volume samples, thus saving time and manpower. b Heatmap shows pair-wise Pearson correlation coefficients and the inset shows multidimensional scaling to visualize the level of similarity between the P. vinckei microsamples. Microsamples show low degree of variability and are highly reproducible as proved by tight correlations between biological replicates. c High Pearson correlations were observed between normalized gene expression values (shown as logarithm of fragments per kilobase of transcript per million mapped reads) from microsampling (x-axis) and terminal blood sampling (y-axis) methods. d Bioanalyser electrophoregrams of total RNA from Plasmodium vinckei vinckei CY microsamples show that high quality RNA could be extracted consistently from 20 μL microsamples

    Journal: Malaria Journal

    Article Title: A fast and cost-effective microsampling protocol incorporating reduced animal usage for time-series transcriptomics in rodent malaria parasites

    doi: 10.1186/s12936-019-2659-4

    Figure Lengend Snippet: Microsampling protocol design and reproducibility. a In terminal blood sampling, at each time point, groups of mice are exsanguinated to obtain 0.5–0.6 mL blood volumes, which are then subject to leukocyte depletion and saponin lysis before TRIzol treatment. Thus, the number of mice increases proportionally with the number of biological replicates and time points in the study design (number of mice per timepoint X number of timepoints; NT). Microsampling involves obtaining sample volumes as low as 20 μL from the same mouse at different time points, thus confining the number of mice to just biological replicates (N) and significantly lowering costs and biological variability. Leukocyte depletion and saponin lysis are also not performed on the low volume samples, thus saving time and manpower. b Heatmap shows pair-wise Pearson correlation coefficients and the inset shows multidimensional scaling to visualize the level of similarity between the P. vinckei microsamples. Microsamples show low degree of variability and are highly reproducible as proved by tight correlations between biological replicates. c High Pearson correlations were observed between normalized gene expression values (shown as logarithm of fragments per kilobase of transcript per million mapped reads) from microsampling (x-axis) and terminal blood sampling (y-axis) methods. d Bioanalyser electrophoregrams of total RNA from Plasmodium vinckei vinckei CY microsamples show that high quality RNA could be extracted consistently from 20 μL microsamples

    Article Snippet: The RNA pellet was resuspended in 15 μL nuclease-free water, RNA quantity measured by Qubit fluorometer and RNA integrity measured by Agilent Bioanalyser (Agilent RNA 6000 Nano kit Cat#5067-1511).

    Techniques: Sampling, Mouse Assay, Lysis, Expressing

    Microsampling protocol design and reproducibility. a In terminal blood sampling, at each time point, groups of mice are exsanguinated to obtain 0.5–0.6 mL blood volumes, which are then subject to leukocyte depletion and saponin lysis before TRIzol treatment. Thus, the number of mice increases proportionally with the number of biological replicates and time points in the study design (number of mice per timepoint X number of timepoints; NT). Microsampling involves obtaining sample volumes as low as 20 μL from the same mouse at different time points, thus confining the number of mice to just biological replicates (N) and significantly lowering costs and biological variability. Leukocyte depletion and saponin lysis are also not performed on the low volume samples, thus saving time and manpower. b Heatmap shows pair-wise Pearson correlation coefficients and the inset shows multidimensional scaling to visualize the level of similarity between the P. vinckei microsamples. Microsamples show low degree of variability and are highly reproducible as proved by tight correlations between biological replicates. c High Pearson correlations were observed between normalized gene expression values (shown as logarithm of fragments per kilobase of transcript per million mapped reads) from microsampling (x-axis) and terminal blood sampling (y-axis) methods. d Bioanalyser electrophoregrams of total RNA from Plasmodium vinckei vinckei CY microsamples show that high quality RNA could be extracted consistently from 20 μL microsamples

    Journal: Malaria Journal

    Article Title: A fast and cost-effective microsampling protocol incorporating reduced animal usage for time-series transcriptomics in rodent malaria parasites

    doi: 10.1186/s12936-019-2659-4

    Figure Lengend Snippet: Microsampling protocol design and reproducibility. a In terminal blood sampling, at each time point, groups of mice are exsanguinated to obtain 0.5–0.6 mL blood volumes, which are then subject to leukocyte depletion and saponin lysis before TRIzol treatment. Thus, the number of mice increases proportionally with the number of biological replicates and time points in the study design (number of mice per timepoint X number of timepoints; NT). Microsampling involves obtaining sample volumes as low as 20 μL from the same mouse at different time points, thus confining the number of mice to just biological replicates (N) and significantly lowering costs and biological variability. Leukocyte depletion and saponin lysis are also not performed on the low volume samples, thus saving time and manpower. b Heatmap shows pair-wise Pearson correlation coefficients and the inset shows multidimensional scaling to visualize the level of similarity between the P. vinckei microsamples. Microsamples show low degree of variability and are highly reproducible as proved by tight correlations between biological replicates. c High Pearson correlations were observed between normalized gene expression values (shown as logarithm of fragments per kilobase of transcript per million mapped reads) from microsampling (x-axis) and terminal blood sampling (y-axis) methods. d Bioanalyser electrophoregrams of total RNA from Plasmodium vinckei vinckei CY microsamples show that high quality RNA could be extracted consistently from 20 μL microsamples

    Article Snippet: The RNA pellet was resuspended in 15 μL nuclease-free water, RNA quantity measured by Qubit fluorometer and RNA integrity measured by Agilent Bioanalyser (Agilent RNA 6000 Nano kit Cat#5067-1511).

    Techniques: Sampling, Mouse Assay, Lysis, Expressing

    Alternative splicing of FOX2 affecting exons in the C-terminal region . (a) Exon structure (green: constitutive exons; orange: annotated cassette exons; purple: putative cassette exon predicted by orthology), location of RT-PCR primers (green arrows), and unique restriction enzyme cleavage sites (exon and primer sizes are shown to scale, introns not to scale). (b) Verification of RT-PCR product sizes generated from paired samples of adenocarcinoma of NSCLC and NAT of six patients (ø: no template control). (c) RT-PCR product sizes analysed using the Bioanalyzer. (d) Cleavage by Hpy8I indicated presence of the first cassette exon. (e) No cleavage by AfeI indicated absence of the second cassette exon. (f) No cleavage by HinfI indicated absence of the third cassette exon.

    Journal: BMC Genomics

    Article Title: Exon Array Analysis using re-defined probe sets results in reliable identification of alternatively spliced genes in non-small cell lung cancer

    doi: 10.1186/1471-2164-11-676

    Figure Lengend Snippet: Alternative splicing of FOX2 affecting exons in the C-terminal region . (a) Exon structure (green: constitutive exons; orange: annotated cassette exons; purple: putative cassette exon predicted by orthology), location of RT-PCR primers (green arrows), and unique restriction enzyme cleavage sites (exon and primer sizes are shown to scale, introns not to scale). (b) Verification of RT-PCR product sizes generated from paired samples of adenocarcinoma of NSCLC and NAT of six patients (ø: no template control). (c) RT-PCR product sizes analysed using the Bioanalyzer. (d) Cleavage by Hpy8I indicated presence of the first cassette exon. (e) No cleavage by AfeI indicated absence of the second cassette exon. (f) No cleavage by HinfI indicated absence of the third cassette exon.

    Article Snippet: Concentration of total RNA was quantified using a NanoDrop spectrometer and quality of RNA was measured on an Agilent Bioanalyzer (Agilent RNA 6000 Pico and Nano Kit; Agilent Technologies, Santa Clara, CA, USA).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Generated