Structured Review

Agilent technologies bioanalyzer rna 6000 pico kit
Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using Agilent 2100 <t>Bioanalyzer</t> with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.
Bioanalyzer Rna 6000 Pico Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bioanalyzer rna 6000 pico kit/product/Agilent technologies
Average 94 stars, based on 4 article reviews
Price from $9.99 to $1999.99
bioanalyzer rna 6000 pico kit - by Bioz Stars, 2019-12
94/100 stars

Images

1) Product Images from "Ensuring good quality rna for quantitative real-time pcr isolated from renal proximal tubular cells using laser capture microdissection"

Article Title: Ensuring good quality rna for quantitative real-time pcr isolated from renal proximal tubular cells using laser capture microdissection

Journal: BMC Research Notes

doi: 10.1186/1756-0500-7-62

Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using Agilent 2100 Bioanalyzer with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.
Figure Legend Snippet: Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using Agilent 2100 Bioanalyzer with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.

Techniques Used: Concentration Assay, Produced

Related Articles

Centrifugation:

Article Title: Polymerase pausing induced by sequence-specific RNA-binding protein drives heterochromatin assembly
Article Snippet: Following centrifugation at 14,000 rpm for 10 min at 4°C, the supernatant was transferred to a microcentrifuge tube, extracted once with chloroform, and precipitated with isopropanol. .. Input RNA quality and mRNA purity were verified by Bioanalyzer RNA 6000 Pico kit (Agilent, 5067-1513).

Article Title: Diguanylate Cyclases AdrA and STM1987 Regulate Salmonella enterica Exopolysaccharide Production during Plant Colonization in an Environment-Dependent Manner
Article Snippet: At 24 hpi, 10-ml samples containing planktonic bacteria from three petri dishes were combined (for 30 ml total per treatment) in cold Oakridge tubes containing 1/5 volume ice-cold stop solution (5% phenol in EtOH) and pelleted by centrifugation at 10,000 × g at 4°C. .. RNA quality was confirmed using Bio-Rad Experion RNA StdSens or Agilent Bioanalyzer RNA 6000 Pico kit protocols.

Amplification:

Article Title: TCF21 and the environmental sensor aryl-hydrocarbon receptor cooperate to activate a pro-inflammatory gene expression program in coronary artery smooth muscle cells
Article Snippet: Paragraph title: RNA extraction from laser capture microdissection and cDNA amplification ... Total RNA was extracted using RNeasy Plus Micro kit (Qiagen), and the quality of RNA checked with Agilient Bioanalyzer RNA 6000 Pico kit.

Article Title: Genetic and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes
Article Snippet: RNA integrity number determined using the Bioanalyzer RNA 6000 Pico Kit (Agilent) for all samples was 10. .. First PCR was performed using cDNA equivalent to 2.5 ng of RNA and Platinum SuperFi DNA Polymerase (Invitrogen) with the following primers: M1SS: 5′-AAGCAGTGGTATCAACGCA-3′, mIGG12_r2: 5′-ATTGGGCAGCCCTGATTAGTGGATAGACHGATG-3′, mIGG3_r2: 5′-ATTGGGCAGCCCTGATTAAGGGATAGACAGATG -3′, mIGM_r2: 5′- ATTGGGCAGCCCTGATTGGGGGAAGACATTTGG-3′, mIGD_r2: 5′-ATTGGGCAGCCCTGATTCTCTGAGAGGAGGAAC-3′.

Article Title: Exosomes impact survival to radiation exposure in cell line models of nervous system cancer
Article Snippet: The extracted RNAs were quantified and quality checked using a BioAnalyzer RNA 6000 Pico Kit (Agilent Technologies). .. The extracted RNAs were quantified and quality checked using a BioAnalyzer RNA 6000 Pico Kit (Agilent Technologies).

Blocking Assay:

Article Title: High-throughput, Efficient, and Unbiased Capture of Small RNAs from Low-input Samples for Sequencing
Article Snippet: With the optimized method presented here, miRNA libraries were successfully prepared from plasma samples with RNA levels undetectable by the Thermo Scientific Nanodrop One ( < 1.6 ng/μL) or Agilent Bioanalyzer RNA 6000 Pico kit ( < 50 pg/μL). .. The 5′ and the 3′ adapters included four degenerate bases at the ligation site of each.

Article Title: A Laboratory Methodology for Dual RNA-Sequencing of Bacteria and their Host Cells In Vitro
Article Snippet: Isopropanol (Sigma-Aldrich, cat. no. I9516) Poly(A)Purist Mag Purification Kit (Thermo Fisher, cat. no. AM1922) TURBO DNA-free TM kit (Thermo Fisher, cat. no. AM1907) DEPC-treated water (Thermo Fisher, cat. no. AM9915G) MasterPureTM Complete DNA and RNA Purification Kit (Epicenter, cat. no. MC85200) RNA Clean & ConcentratorTM -5 (Zymo Research, cat. no. R1015) Ribo-ZeroTM rRNA Removal Kit (Human/Mouse/Rat) (Epicenter, cat. no. RZH1046) Ribo-ZeroTM rRNA Removal Kit (Gram-negative bacteria) (Epicenter, cat. no. RZNB1056) Sonicator S-4000 (Misonix, cat. no. S4000) (for use when lysing Gram positive bacteria) TaqMan® Gene Expression Assay (Thermo Fisher, cat. no. 4453320) High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher, cat. no. 4368814) Beta actin probe (Thermo Fisher, cat. no. Hs99999903_m1) Mitochondrially encoded ATP synthase 6 probe (Thermo Fisher, cat. no. Hs02596862_g1) Eukaryotic 18S rRNA probe (Thermo Fisher, cat. no. Hs99999901_s1) .. MicroAmp® optical 96-well reaction plates (Thermo Fisher, cat. no. 4306737) MicroAmp® optical adhesive film (Thermo Fisher, cat. no. 4311971) MicroAmp® optical film compression pad (Thermo Fisher, cat. no. 4312639) Flask rocker (Grant Instruments, PS-M3D) DNase and RNase-free 1.5 mL microcentrifuge tubes (Sarstedt, cat. no. 72.692.210) Refrigerated microcentrifuge (Beckman Coulter, 20R) Centrifuge (Beckman Coulter, X-12R) Vortex (Scientific Industries, G-560E) Heating block (x2) (Thermo Fisher, cat. no. 2001Q) Ice Ice bucket Timer Real-time qPCR machine (Applied Biosystems, 7900HT) Temperature cycler (Bio-Rad, C1000) Incubator, 37°C, 5% CO2 (Sanyo, MCO-19AIC) Bioanalyzer RNA 6000 Pico Kit (Agilent, cat. no. 5067-1513) 6-well plates (Thermo Scientific, cat. no. 140675) Cell scrapers (Sarstedt, cat. no. 83.1830) 15 mL centrifuge tubes (Thermo Scientific, cat. no. 339652) .. 70% ethanol Combine 350 mL ethanol with 150 mL nuclease free water.

Real-time Polymerase Chain Reaction:

Article Title: Ensuring good quality rna for quantitative real-time pcr isolated from renal proximal tubular cells using laser capture microdissection
Article Snippet: Paragraph title: Minimum number of rat renal PTCs required to yield sufficient total RNA for downstream qPCR ... The RNA concentrations were measured using Agilent Bioanalyzer RNA 6000 Pico kit.

Article Title: In Search for Reliable Markers of Glioma-Induced Polarization of Microglia
Article Snippet: The expression of selected genes were tested by qPCR with commercial primers for the following genes P2RY12, SIGLEC1, SELPLG, IFITM5, PTAFR, TNFRSF1B , and GPR84 , purchased from ScienCell Research Laboratories (sequences undisclosed). .. Samples were run on Bioanalyzer RNA 6000 Pico kit (Agilent, USA) to assess RNA integrity and concentration.

Article Title: A Laboratory Methodology for Dual RNA-Sequencing of Bacteria and their Host Cells In Vitro
Article Snippet: Isopropanol (Sigma-Aldrich, cat. no. I9516) Poly(A)Purist Mag Purification Kit (Thermo Fisher, cat. no. AM1922) TURBO DNA-free TM kit (Thermo Fisher, cat. no. AM1907) DEPC-treated water (Thermo Fisher, cat. no. AM9915G) MasterPureTM Complete DNA and RNA Purification Kit (Epicenter, cat. no. MC85200) RNA Clean & ConcentratorTM -5 (Zymo Research, cat. no. R1015) Ribo-ZeroTM rRNA Removal Kit (Human/Mouse/Rat) (Epicenter, cat. no. RZH1046) Ribo-ZeroTM rRNA Removal Kit (Gram-negative bacteria) (Epicenter, cat. no. RZNB1056) Sonicator S-4000 (Misonix, cat. no. S4000) (for use when lysing Gram positive bacteria) TaqMan® Gene Expression Assay (Thermo Fisher, cat. no. 4453320) High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher, cat. no. 4368814) Beta actin probe (Thermo Fisher, cat. no. Hs99999903_m1) Mitochondrially encoded ATP synthase 6 probe (Thermo Fisher, cat. no. Hs02596862_g1) Eukaryotic 18S rRNA probe (Thermo Fisher, cat. no. Hs99999901_s1) .. MicroAmp® optical 96-well reaction plates (Thermo Fisher, cat. no. 4306737) MicroAmp® optical adhesive film (Thermo Fisher, cat. no. 4311971) MicroAmp® optical film compression pad (Thermo Fisher, cat. no. 4312639) Flask rocker (Grant Instruments, PS-M3D) DNase and RNase-free 1.5 mL microcentrifuge tubes (Sarstedt, cat. no. 72.692.210) Refrigerated microcentrifuge (Beckman Coulter, 20R) Centrifuge (Beckman Coulter, X-12R) Vortex (Scientific Industries, G-560E) Heating block (x2) (Thermo Fisher, cat. no. 2001Q) Ice Ice bucket Timer Real-time qPCR machine (Applied Biosystems, 7900HT) Temperature cycler (Bio-Rad, C1000) Incubator, 37°C, 5% CO2 (Sanyo, MCO-19AIC) Bioanalyzer RNA 6000 Pico Kit (Agilent, cat. no. 5067-1513) 6-well plates (Thermo Scientific, cat. no. 140675) Cell scrapers (Sarstedt, cat. no. 83.1830) 15 mL centrifuge tubes (Thermo Scientific, cat. no. 339652) .. 70% ethanol Combine 350 mL ethanol with 150 mL nuclease free water.

Article Title: Diguanylate Cyclases AdrA and STM1987 Regulate Salmonella enterica Exopolysaccharide Production during Plant Colonization in an Environment-Dependent Manner
Article Snippet: Real-time PCR was used to measure potential DNA contamination using 100 ng of RNA, SsoFast EvaGreen Supermix (Bio-Rad, Hercules, CA), and STM1987 primers ( ). .. RNA quality was confirmed using Bio-Rad Experion RNA StdSens or Agilent Bioanalyzer RNA 6000 Pico kit protocols.

Incubation:

Article Title: Solid phase chemistry to covalently and reversibly capture thiolated RNA
Article Snippet: RNA was eluted with 10 μl elution buffer (100 mM NaCl, 10 mM Tris pH 7.4, 1 mM EDTA, 10 mM DTT and 100 pg fragmented total RNA from S. pombe (a generous gift from the Berro lab, Yale University) and incubated at room temperature in the dark for 15 min with rotation. .. Eluent was ethanol precipitated with 5–10 μg glycoblue, and RNA concentration was assayed by Bioanalyzer RNA 6000 Pico Kit (Agilent) according to the manufacturer's instructions.

Article Title: Genetic and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes
Article Snippet: RNA integrity number determined using the Bioanalyzer RNA 6000 Pico Kit (Agilent) for all samples was 10. .. Total RNA was reverse transcribed using SMARTscribe Reverse Transcriptase (Takara) with IgH isotype-specific primers (1 μM each): IgHG1/2: 5′-CAGGGATCCAKAGTTC-3′, IgHG3: 5′-CAGGGCTCCATAGTTC-3′, IgHM: 5′-GATGACTTCAGTGTTGT-3′ and IgHD: 5′-AGTGGCTGACTTCCAA-3′; and 1μM template-switch adapter (5′-AAGCAGUGGTAUCAACGCAGAGUNNNNUNNNNUNNNNUCTTrGrGrGrG-3′, U=deoxyuridine) to introduce a unique molecular identifier (UMI) sequence to the 3′-end of each cDNA molecule.

Article Title: Remedial Treatment of Corroded Iron Objects by Environmental Aeromonas Isolates
Article Snippet: To remove rRNA, the Ribo-Zero mix incubated previously was mixed with the prepared magnetic beads and placed at room temperature for 5 min and then at 50°C for 5 min. .. The final mRNA eluted was quantified using the Qubit-IT RNA HS assay kit (Thermo Fisher Scientific) and Bioanalyzer RNA 6000 Pico kit (Agilent). (ii) Illumina indexed library preparation.

Stripping Membranes:

Article Title: In Search for Reliable Markers of Glioma-Induced Polarization of Microglia
Article Snippet: Global gene expression analysis using Affymetrix MG-430 PM Strip system (Affymetrix, USA) using GCM treated primary microglia for 6 h (n = 3). .. Samples were run on Bioanalyzer RNA 6000 Pico kit (Agilent, USA) to assess RNA integrity and concentration.

Expressing:

Article Title: In Search for Reliable Markers of Glioma-Induced Polarization of Microglia
Article Snippet: Paragraph title: Gene Expression Analysis ... Samples were run on Bioanalyzer RNA 6000 Pico kit (Agilent, USA) to assess RNA integrity and concentration.

Article Title: High-throughput, Efficient, and Unbiased Capture of Small RNAs from Low-input Samples for Sequencing
Article Snippet: This many-to-one sample approach was further optimized to obtain miRNA expression data from human plasma samples. .. With the optimized method presented here, miRNA libraries were successfully prepared from plasma samples with RNA levels undetectable by the Thermo Scientific Nanodrop One ( < 1.6 ng/μL) or Agilent Bioanalyzer RNA 6000 Pico kit ( < 50 pg/μL).

Article Title: The Ox40/Ox40 Ligand Pathway Promotes Pathogenic Th Cell Responses, Plasmablast Accumulation, and Lupus Nephritis in NZB/W F1 Mice
Article Snippet: For all RNASeq experiments, a Nanodrop 8000 (Thermo Scientific) was used to quantify RNA, and integrity was measured using the Bioanalyzer RNA 6000 Pico Kit (Agilent). .. For all RNASeq experiments, a Nanodrop 8000 (Thermo Scientific) was used to quantify RNA, and integrity was measured using the Bioanalyzer RNA 6000 Pico Kit (Agilent).

Modification:

Article Title: High-throughput, Efficient, and Unbiased Capture of Small RNAs from Low-input Samples for Sequencing
Article Snippet: With the optimized method presented here, miRNA libraries were successfully prepared from plasma samples with RNA levels undetectable by the Thermo Scientific Nanodrop One ( < 1.6 ng/μL) or Agilent Bioanalyzer RNA 6000 Pico kit ( < 50 pg/μL). .. The 5′ and the 3′ adapters included four degenerate bases at the ligation site of each.

Hybridization:

Article Title: In Search for Reliable Markers of Glioma-Induced Polarization of Microglia
Article Snippet: Samples were run on Bioanalyzer RNA 6000 Pico kit (Agilent, USA) to assess RNA integrity and concentration. .. Samples were run on Bioanalyzer RNA 6000 Pico kit (Agilent, USA) to assess RNA integrity and concentration.

High Performance Liquid Chromatography:

Article Title: Single-Cell Semiconductor Sequencing
Article Snippet: Agilent Bioanalyzer™ RNA 6000 Pico Kit (Cat # 5067-1513, Agilent Technologies). .. Agilent Bioanalyzer™ RNA 6000 Pico Kit (Cat # 5067-1513, Agilent Technologies).

Flow Cytometry:

Article Title: Impairments of spatial learning and memory following intrahippocampal injection in rats of 3-mercaptopropionic acid-modified CdTe quantum dots and molecular mechanisms
Article Snippet: RNA integrity number was determined for each sample using a Bioanalyzer RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA, USA). .. Each library was loaded into one lane of the Illumina HiSeq 2500 for 2×125 bp pair-end sequencing.

Ligation:

Article Title: High-throughput, Efficient, and Unbiased Capture of Small RNAs from Low-input Samples for Sequencing
Article Snippet: The method we present incorporates each of these improvements while using barcodes to allow sample pooling after the first ligation . .. With the optimized method presented here, miRNA libraries were successfully prepared from plasma samples with RNA levels undetectable by the Thermo Scientific Nanodrop One ( < 1.6 ng/μL) or Agilent Bioanalyzer RNA 6000 Pico kit ( < 50 pg/μL).

Article Title: Exosomes impact survival to radiation exposure in cell line models of nervous system cancer
Article Snippet: The extracted RNAs were quantified and quality checked using a BioAnalyzer RNA 6000 Pico Kit (Agilent Technologies). .. The extracted RNAs were quantified and quality checked using a BioAnalyzer RNA 6000 Pico Kit (Agilent Technologies).

Introduce:

Article Title: Genetic and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes
Article Snippet: RNA integrity number determined using the Bioanalyzer RNA 6000 Pico Kit (Agilent) for all samples was 10. .. RNA integrity number determined using the Bioanalyzer RNA 6000 Pico Kit (Agilent) for all samples was 10.

Generated:

Article Title: Transcriptomic Analysis of the Campylobacter jejuni Response to T4-Like Phage NCTC 12673 Infection
Article Snippet: Total mRNA libraries from all replicates were generated. .. Successful rRNA depletion was confirmed using the Agilent Bioanalyzer RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA, USA).

other:

Article Title: Single-Neuron Transcriptome and Methylome Sequencing for Epigenomic Analysis of Aging
Article Snippet: Agilent Bioanalyzer™ RNA 6000 Pico Kit (Cat #5067-1513, Agilent Technologies).

Imaging:

Article Title: In Search for Reliable Markers of Glioma-Induced Polarization of Microglia
Article Snippet: Samples were run on Bioanalyzer RNA 6000 Pico kit (Agilent, USA) to assess RNA integrity and concentration. .. Samples were run on Bioanalyzer RNA 6000 Pico kit (Agilent, USA) to assess RNA integrity and concentration.

Sequencing:

Article Title: Impairments of spatial learning and memory following intrahippocampal injection in rats of 3-mercaptopropionic acid-modified CdTe quantum dots and molecular mechanisms
Article Snippet: Paragraph title: Transcriptome-library preparation and sequencing ... RNA integrity number was determined for each sample using a Bioanalyzer RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA, USA).

Article Title: High-throughput, Efficient, and Unbiased Capture of Small RNAs from Low-input Samples for Sequencing
Article Snippet: There are few commercially available miRNA sequencing kits optimized for low-input RNA samples. .. With the optimized method presented here, miRNA libraries were successfully prepared from plasma samples with RNA levels undetectable by the Thermo Scientific Nanodrop One ( < 1.6 ng/μL) or Agilent Bioanalyzer RNA 6000 Pico kit ( < 50 pg/μL).

Article Title: Polymerase pausing induced by sequence-specific RNA-binding protein drives heterochromatin assembly
Article Snippet: Input RNA quality and mRNA purity were verified by Bioanalyzer RNA 6000 Pico kit (Agilent, 5067-1513). .. Input RNA quality and mRNA purity were verified by Bioanalyzer RNA 6000 Pico kit (Agilent, 5067-1513).

Article Title: Transcriptomic Analysis of the Campylobacter jejuni Response to T4-Like Phage NCTC 12673 Infection
Article Snippet: Successful rRNA depletion was confirmed using the Agilent Bioanalyzer RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA, USA). .. Successful rRNA depletion was confirmed using the Agilent Bioanalyzer RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA, USA).

Binding Assay:

Article Title: Solid phase chemistry to covalently and reversibly capture thiolated RNA
Article Snippet: Beads were incubated for 5 min with the following wash buffers: once with 100 μl 1× binding buffer, twice with 100 μl high salt wash buffer (1 M NaCl, 100 mM Tris pH 7.4, 10 mM EDTA, 0.05% Tween), twice with 100 μl denaturing buffer (8 M guanidinium chloride) and three times with 100 μl buffer TE (10 mM Tris pH 7.4, 1 mM EDTA) at 55°C. .. Eluent was ethanol precipitated with 5–10 μg glycoblue, and RNA concentration was assayed by Bioanalyzer RNA 6000 Pico Kit (Agilent) according to the manufacturer's instructions.

In Vivo:

Article Title: The Ox40/Ox40 Ligand Pathway Promotes Pathogenic Th Cell Responses, Plasmablast Accumulation, and Lupus Nephritis in NZB/W F1 Mice
Article Snippet: For in vivo treatment experiments, live CD45+ CD19− CD3+ CD4+ T cells were sorted to high purity from the spleen ( n = 4) and kidney ( n = 5) after 1 wk (day 8) of anti-Ox40 agonist mAb treatment, followed by lysing with RLT buffer supplemented with 2-ME (Sigma-Aldrich). .. For all RNASeq experiments, a Nanodrop 8000 (Thermo Scientific) was used to quantify RNA, and integrity was measured using the Bioanalyzer RNA 6000 Pico Kit (Agilent).

RNA Sequencing Assay:

Article Title: Polymerase pausing induced by sequence-specific RNA-binding protein drives heterochromatin assembly
Article Snippet: Paragraph title: RNA-seq ... Input RNA quality and mRNA purity were verified by Bioanalyzer RNA 6000 Pico kit (Agilent, 5067-1513).

Article Title: Transcriptome analysis of Pseudomonas aeruginosa PAO1 grown at both body and elevated temperatures
Article Snippet: The quality of the extracted RNA samples was also determined using an Agilent Bioanalyzer-RNA 6000 Pico Kit (Agilent Technologies, CA, USA). .. The quality of the extracted RNA samples was also determined using an Agilent Bioanalyzer-RNA 6000 Pico Kit (Agilent Technologies, CA, USA).

Article Title: Transcriptomic Analysis of the Campylobacter jejuni Response to T4-Like Phage NCTC 12673 Infection
Article Snippet: Paragraph title: 2.4. RNA Sequencing ... Successful rRNA depletion was confirmed using the Agilent Bioanalyzer RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA, USA).

RNA HS Assay:

Article Title: Remedial Treatment of Corroded Iron Objects by Environmental Aeromonas Isolates
Article Snippet: The rRNA-depleted samples were purified using the RNeasy MinElute kit (Qiagen). .. The final mRNA eluted was quantified using the Qubit-IT RNA HS assay kit (Thermo Fisher Scientific) and Bioanalyzer RNA 6000 Pico kit (Agilent). (ii) Illumina indexed library preparation. .. The preparation of indexed libraries from RNA and sequencing was performed using Illumina’s ScriptSeq v2 sample preparation kit.

Magnetic Beads:

Article Title: Remedial Treatment of Corroded Iron Objects by Environmental Aeromonas Isolates
Article Snippet: To remove rRNA, the Ribo-Zero mix incubated previously was mixed with the prepared magnetic beads and placed at room temperature for 5 min and then at 50°C for 5 min. .. The final mRNA eluted was quantified using the Qubit-IT RNA HS assay kit (Thermo Fisher Scientific) and Bioanalyzer RNA 6000 Pico kit (Agilent). (ii) Illumina indexed library preparation.

Article Title: Single-Cell Semiconductor Sequencing
Article Snippet: Dynabeads® MyOne™ Streptavidin C1 Magnetic Beads (Cat # 65001, Invitrogen, Life Technologies). .. Agilent Bioanalyzer™ RNA 6000 Pico Kit (Cat # 5067-1513, Agilent Technologies).

Isolation:

Article Title: Ensuring good quality rna for quantitative real-time pcr isolated from renal proximal tubular cells using laser capture microdissection
Article Snippet: Total RNA was then isolated using PicoPure® RNA Isolation Kit (Life Technologies) according to the manufacturer’s protocol with some modifications. .. The RNA concentrations were measured using Agilent Bioanalyzer RNA 6000 Pico kit.

Article Title: Exosomes impact survival to radiation exposure in cell line models of nervous system cancer
Article Snippet: Exosomes from flasks were isolated and run for RNA exploration analysis. .. The extracted RNAs were quantified and quality checked using a BioAnalyzer RNA 6000 Pico Kit (Agilent Technologies).

Article Title: Strand-specific RNA sequencing in Plasmodium falciparum malaria identifies developmentally regulated long non-coding RNA and circular RNA
Article Snippet: During both RNeasy Midi and Mini RNA extraction procedures, we performed the optional on-column DNase I digestion for thirty minutes to remove genomic DNA. .. We stored isolated total RNA aliquots at −80 °C with 1 unit/uL RNaseOUT (Invitrogen), and validated RNA quality using an Agilent Bioanalyzer RNA 6000 Pico Kit. .. We began library preparation with a second DNase treatment (Ambion TURBO DNase) using 20 units of SUPERase-In (Ambion) and 40 units of RNaseOUT (Invitrogen) to protect RNA from degradation.

Article Title: Diguanylate Cyclases AdrA and STM1987 Regulate Salmonella enterica Exopolysaccharide Production during Plant Colonization in an Environment-Dependent Manner
Article Snippet: Paragraph title: RNA isolation. ... RNA quality was confirmed using Bio-Rad Experion RNA StdSens or Agilent Bioanalyzer RNA 6000 Pico kit protocols.

Size-exclusion Chromatography:

Article Title: Polymerase pausing induced by sequence-specific RNA-binding protein drives heterochromatin assembly
Article Snippet: Zirconia-silica beads (0.5-mm; BioSpec, 11079105z) were added, and lysis was accomplished by three cycles of bead beating for 90 sec on high (Bead Ruptor 12 homogenizer, OMNI International). .. Input RNA quality and mRNA purity were verified by Bioanalyzer RNA 6000 Pico kit (Agilent, 5067-1513).

Microscopy:

Article Title: TCF21 and the environmental sensor aryl-hydrocarbon receptor cooperate to activate a pro-inflammatory gene expression program in coronary artery smooth muscle cells
Article Snippet: 7um cryosections were placed on to Leica membrane slides, then visualized under the microscope for LCM. .. Total RNA was extracted using RNeasy Plus Micro kit (Qiagen), and the quality of RNA checked with Agilient Bioanalyzer RNA 6000 Pico kit.

Mouse Assay:

Article Title: Genetic and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes
Article Snippet: Aicda-/- mice served as controls. .. RNA integrity number determined using the Bioanalyzer RNA 6000 Pico Kit (Agilent) for all samples was 10.

Polymerase Chain Reaction:

Article Title: TCF21 and the environmental sensor aryl-hydrocarbon receptor cooperate to activate a pro-inflammatory gene expression program in coronary artery smooth muscle cells
Article Snippet: Total RNA was extracted using RNeasy Plus Micro kit (Qiagen), and the quality of RNA checked with Agilient Bioanalyzer RNA 6000 Pico kit. .. Using the published SMART-Seq2 protocol [ ], we amplified the ultra-low input RNA from the LCM.

Article Title: Genetic and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes
Article Snippet: RNA integrity number determined using the Bioanalyzer RNA 6000 Pico Kit (Agilent) for all samples was 10. .. RNA integrity number determined using the Bioanalyzer RNA 6000 Pico Kit (Agilent) for all samples was 10.

Article Title: Exosomes impact survival to radiation exposure in cell line models of nervous system cancer
Article Snippet: The extracted RNAs were quantified and quality checked using a BioAnalyzer RNA 6000 Pico Kit (Agilent Technologies). .. The extracted RNAs were quantified and quality checked using a BioAnalyzer RNA 6000 Pico Kit (Agilent Technologies).

Construct:

Article Title: Polymerase pausing induced by sequence-specific RNA-binding protein drives heterochromatin assembly
Article Snippet: Input RNA quality and mRNA purity were verified by Bioanalyzer RNA 6000 Pico kit (Agilent, 5067-1513). .. Input RNA quality and mRNA purity were verified by Bioanalyzer RNA 6000 Pico kit (Agilent, 5067-1513).

cDNA Library Assay:

Article Title: Transcriptome analysis of Pseudomonas aeruginosa PAO1 grown at both body and elevated temperatures
Article Snippet: The quality of the extracted RNA samples was also determined using an Agilent Bioanalyzer-RNA 6000 Pico Kit (Agilent Technologies, CA, USA). .. Synthesis of cDNA was performed using the protocols of ScriptSeq™ v2 RNA-seq Library Preparation Kit (Epicentre, WI, USA).

Chloramphenicol Acetyltransferase Assay:

Article Title: Exosomes impact survival to radiation exposure in cell line models of nervous system cancer
Article Snippet: Total exosomal RNA was extracted using mirVana kit (Ambion, cat:AM1560). .. The extracted RNAs were quantified and quality checked using a BioAnalyzer RNA 6000 Pico Kit (Agilent Technologies).

Purification:

Article Title: Solid phase chemistry to covalently and reversibly capture thiolated RNA
Article Snippet: Paragraph title: Purification of s4 U-RNA with MTS-resin ... Eluent was ethanol precipitated with 5–10 μg glycoblue, and RNA concentration was assayed by Bioanalyzer RNA 6000 Pico Kit (Agilent) according to the manufacturer's instructions.

Article Title: Genetic and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes
Article Snippet: Total RNA was purified using RNeasy Micro Kit (Qiagen). .. RNA integrity number determined using the Bioanalyzer RNA 6000 Pico Kit (Agilent) for all samples was 10.

Article Title: Transcriptome analysis of Pseudomonas aeruginosa PAO1 grown at both body and elevated temperatures
Article Snippet: Total RNA was extracted using MasterPure™ RNA Purification Kit (Epicentre, WI, USA) as per the manufacturer’s instructions. .. The quality of the extracted RNA samples was also determined using an Agilent Bioanalyzer-RNA 6000 Pico Kit (Agilent Technologies, CA, USA).

Article Title: Remedial Treatment of Corroded Iron Objects by Environmental Aeromonas Isolates
Article Snippet: The rRNA-depleted samples were purified using the RNeasy MinElute kit (Qiagen). .. The final mRNA eluted was quantified using the Qubit-IT RNA HS assay kit (Thermo Fisher Scientific) and Bioanalyzer RNA 6000 Pico kit (Agilent). (ii) Illumina indexed library preparation.

Article Title: Single-Cell Semiconductor Sequencing
Article Snippet: Agilent Bioanalyzer™ RNA 6000 Pico Kit (Cat # 5067-1513, Agilent Technologies). .. Agilent Bioanalyzer™ RNA 6000 Pico Kit (Cat # 5067-1513, Agilent Technologies).

Chromatin Immunoprecipitation:

Article Title: Transcriptome analysis of Pseudomonas aeruginosa PAO1 grown at both body and elevated temperatures
Article Snippet: The quality of the extracted RNA samples was also determined using an Agilent Bioanalyzer-RNA 6000 Pico Kit (Agilent Technologies, CA, USA). .. Synthesis of cDNA was performed using the protocols of ScriptSeq™ v2 RNA-seq Library Preparation Kit (Epicentre, WI, USA).

Article Title: Transcriptomic Analysis of the Campylobacter jejuni Response to T4-Like Phage NCTC 12673 Infection
Article Snippet: Successful rRNA depletion was confirmed using the Agilent Bioanalyzer RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA, USA). .. Successful rRNA depletion was confirmed using the Agilent Bioanalyzer RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA, USA).

Article Title: Single-Cell Semiconductor Sequencing
Article Snippet: Ion 314™ Chip (Cat # 4462923; 8 pack), 316™ Chip (Cat # 4466616; 4 pack), 318™ Chip (Cat # 4466617; 4 pack) (Ion Torrent, Life Technologies). .. Agilent Bioanalyzer™ RNA 6000 Pico Kit (Cat # 5067-1513, Agilent Technologies).

Software:

Article Title: In Search for Reliable Markers of Glioma-Induced Polarization of Microglia
Article Snippet: Samples were run on Bioanalyzer RNA 6000 Pico kit (Agilent, USA) to assess RNA integrity and concentration. .. Hybridization was performed at 45°C for 16 h and fluidics, and imaging was done as described in the GeneAtlas System User’s Guide (P/N 08-0246).

Article Title: Transcriptomic Analysis of the Campylobacter jejuni Response to T4-Like Phage NCTC 12673 Infection
Article Snippet: Successful rRNA depletion was confirmed using the Agilent Bioanalyzer RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA, USA). .. The pooled libraries were templated using the Ion PI Hi-Q kit (Thermo Fisher Scientific, Waltham, MA, USA) and sequenced on an Ion Torrent Proton using the Ion PI Hi-Q sequencing 200 kit (Thermo Fisher Scientific, Waltham, MA, USA) on a single Proton V2 chip.

RNA Extraction:

Article Title: TCF21 and the environmental sensor aryl-hydrocarbon receptor cooperate to activate a pro-inflammatory gene expression program in coronary artery smooth muscle cells
Article Snippet: Paragraph title: RNA extraction from laser capture microdissection and cDNA amplification ... Total RNA was extracted using RNeasy Plus Micro kit (Qiagen), and the quality of RNA checked with Agilient Bioanalyzer RNA 6000 Pico kit.

Article Title: Strand-specific RNA sequencing in Plasmodium falciparum malaria identifies developmentally regulated long non-coding RNA and circular RNA
Article Snippet: Paragraph title: Total RNA extraction ... We stored isolated total RNA aliquots at −80 °C with 1 unit/uL RNaseOUT (Invitrogen), and validated RNA quality using an Agilent Bioanalyzer RNA 6000 Pico Kit.

Article Title: Transcriptome analysis of Pseudomonas aeruginosa PAO1 grown at both body and elevated temperatures
Article Snippet: Paragraph title: RNA extraction and cDNA synthesis ... The quality of the extracted RNA samples was also determined using an Agilent Bioanalyzer-RNA 6000 Pico Kit (Agilent Technologies, CA, USA).

Laser Capture Microdissection:

Article Title: TCF21 and the environmental sensor aryl-hydrocarbon receptor cooperate to activate a pro-inflammatory gene expression program in coronary artery smooth muscle cells
Article Snippet: Paragraph title: RNA extraction from laser capture microdissection and cDNA amplification ... Total RNA was extracted using RNeasy Plus Micro kit (Qiagen), and the quality of RNA checked with Agilient Bioanalyzer RNA 6000 Pico kit.

Spectrophotometry:

Article Title: Transcriptome analysis of Pseudomonas aeruginosa PAO1 grown at both body and elevated temperatures
Article Snippet: Purity of RNA samples was assessed using a NanoDrop 2000 Spectrophotometer (Thermo Scientific, MA, USA) and only samples with A260 /A280 and A260 /A230 values > 2.0 were chosen for further work. .. The quality of the extracted RNA samples was also determined using an Agilent Bioanalyzer-RNA 6000 Pico Kit (Agilent Technologies, CA, USA).

Concentration Assay:

Article Title: In Search for Reliable Markers of Glioma-Induced Polarization of Microglia
Article Snippet: Global gene expression analysis using Affymetrix MG-430 PM Strip system (Affymetrix, USA) using GCM treated primary microglia for 6 h (n = 3). .. Samples were run on Bioanalyzer RNA 6000 Pico kit (Agilent, USA) to assess RNA integrity and concentration. .. The protocol was followed according to the manufacture’s manual using 100 ng of total RNA and samples with RIN > 9.5.

Article Title: Solid phase chemistry to covalently and reversibly capture thiolated RNA
Article Snippet: RNA was eluted with 10 μl elution buffer (100 mM NaCl, 10 mM Tris pH 7.4, 1 mM EDTA, 10 mM DTT and 100 pg fragmented total RNA from S. pombe (a generous gift from the Berro lab, Yale University) and incubated at room temperature in the dark for 15 min with rotation. .. Eluent was ethanol precipitated with 5–10 μg glycoblue, and RNA concentration was assayed by Bioanalyzer RNA 6000 Pico Kit (Agilent) according to the manufacturer's instructions. .. A solution of 100 nmol s4 U nucleoside in 18 μl RNase-free water was mixed with 3 μl 10× binding buffer and 6 μl DMF (total volume 30 μl) was enriched using 1, 2, 5, 10 or 20 μl MTS-resin as above.

Article Title: Genetic and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes
Article Snippet: RNA integrity number determined using the Bioanalyzer RNA 6000 Pico Kit (Agilent) for all samples was 10. .. Total RNA was reverse transcribed using SMARTscribe Reverse Transcriptase (Takara) with IgH isotype-specific primers (1 μM each): IgHG1/2: 5′-CAGGGATCCAKAGTTC-3′, IgHG3: 5′-CAGGGCTCCATAGTTC-3′, IgHM: 5′-GATGACTTCAGTGTTGT-3′ and IgHD: 5′-AGTGGCTGACTTCCAA-3′; and 1μM template-switch adapter (5′-AAGCAGUGGTAUCAACGCAGAGUNNNNUNNNNUNNNNUCTTrGrGrGrG-3′, U=deoxyuridine) to introduce a unique molecular identifier (UMI) sequence to the 3′-end of each cDNA molecule.

Article Title: High-throughput, Efficient, and Unbiased Capture of Small RNAs from Low-input Samples for Sequencing
Article Snippet: With the optimized method presented here, miRNA libraries were successfully prepared from plasma samples with RNA levels undetectable by the Thermo Scientific Nanodrop One ( < 1.6 ng/μL) or Agilent Bioanalyzer RNA 6000 Pico kit ( < 50 pg/μL). .. In addition to the blocking modification already present on the 3′ adapter , a blocking amino modification was added to the 5′ end of the 5′ adapter.

Article Title: Remedial Treatment of Corroded Iron Objects by Environmental Aeromonas Isolates
Article Snippet: The concentration of RNA from the extraction was considered to calculate the amounts of reagents used for the preparation of the magnetic beads and for the treatment of total RNA with Ribo-Zero rRNA removal solution (4 μl rRNA removal solution for 0.25 to 1 μg total RNA per reaction). .. The final mRNA eluted was quantified using the Qubit-IT RNA HS assay kit (Thermo Fisher Scientific) and Bioanalyzer RNA 6000 Pico kit (Agilent). (ii) Illumina indexed library preparation.

Lysis:

Article Title: Polymerase pausing induced by sequence-specific RNA-binding protein drives heterochromatin assembly
Article Snippet: Zirconia-silica beads (0.5-mm; BioSpec, 11079105z) were added, and lysis was accomplished by three cycles of bead beating for 90 sec on high (Bead Ruptor 12 homogenizer, OMNI International). .. Input RNA quality and mRNA purity were verified by Bioanalyzer RNA 6000 Pico kit (Agilent, 5067-1513).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Agilent technologies bioanalyzer
    Alternative splicing of FOX2 affecting exons in the C-terminal region . (a) Exon structure (green: constitutive exons; orange: annotated cassette exons; purple: putative cassette exon predicted by orthology), location of RT-PCR primers (green arrows), and unique restriction enzyme cleavage sites (exon and primer sizes are shown to scale, introns not to scale). (b) Verification of RT-PCR product sizes generated from paired samples of adenocarcinoma of NSCLC and NAT of six patients (ø: no template control). (c) RT-PCR product sizes analysed using the <t>Bioanalyzer.</t> (d) Cleavage by Hpy8I indicated presence of the first cassette exon. (e) No cleavage by AfeI indicated absence of the second cassette exon. (f) No cleavage by HinfI indicated absence of the third cassette exon.
    Bioanalyzer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 1594 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bioanalyzer/product/Agilent technologies
    Average 99 stars, based on 1594 article reviews
    Price from $9.99 to $1999.99
    bioanalyzer - by Bioz Stars, 2019-12
    99/100 stars
      Buy from Supplier

    Image Search Results


    Alternative splicing of FOX2 affecting exons in the C-terminal region . (a) Exon structure (green: constitutive exons; orange: annotated cassette exons; purple: putative cassette exon predicted by orthology), location of RT-PCR primers (green arrows), and unique restriction enzyme cleavage sites (exon and primer sizes are shown to scale, introns not to scale). (b) Verification of RT-PCR product sizes generated from paired samples of adenocarcinoma of NSCLC and NAT of six patients (ø: no template control). (c) RT-PCR product sizes analysed using the Bioanalyzer. (d) Cleavage by Hpy8I indicated presence of the first cassette exon. (e) No cleavage by AfeI indicated absence of the second cassette exon. (f) No cleavage by HinfI indicated absence of the third cassette exon.

    Journal: BMC Genomics

    Article Title: Exon Array Analysis using re-defined probe sets results in reliable identification of alternatively spliced genes in non-small cell lung cancer

    doi: 10.1186/1471-2164-11-676

    Figure Lengend Snippet: Alternative splicing of FOX2 affecting exons in the C-terminal region . (a) Exon structure (green: constitutive exons; orange: annotated cassette exons; purple: putative cassette exon predicted by orthology), location of RT-PCR primers (green arrows), and unique restriction enzyme cleavage sites (exon and primer sizes are shown to scale, introns not to scale). (b) Verification of RT-PCR product sizes generated from paired samples of adenocarcinoma of NSCLC and NAT of six patients (ø: no template control). (c) RT-PCR product sizes analysed using the Bioanalyzer. (d) Cleavage by Hpy8I indicated presence of the first cassette exon. (e) No cleavage by AfeI indicated absence of the second cassette exon. (f) No cleavage by HinfI indicated absence of the third cassette exon.

    Article Snippet: Concentration of total RNA was quantified using a NanoDrop spectrometer and quality of RNA was measured on an Agilent Bioanalyzer (Agilent RNA 6000 Pico and Nano Kit; Agilent Technologies, Santa Clara, CA, USA).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Generated

    (A) Agilent Bioanalyzer analysis (High-sensitivity DNA Kit) of a SPRI-beads-purified DNA library is shown. DNA fragments smaller than 175 bp include library DNAs with very short cDNA inserts, PCR primer dimers, and residual PCR primers. Gel purification

    Journal:

    Article Title: Current Protocols in Molecular Biology Unit 4.23

    doi: 10.1002/0471142727.mb0423s110

    Figure Lengend Snippet: (A) Agilent Bioanalyzer analysis (High-sensitivity DNA Kit) of a SPRI-beads-purified DNA library is shown. DNA fragments smaller than 175 bp include library DNAs with very short cDNA inserts, PCR primer dimers, and residual PCR primers. Gel purification

    Article Snippet: Note: the quality and quantity of RNA fragments can be examined by Agilent Bioanalyzer analysis (RNA 6000 Pico Kit).

    Techniques: Purification, Polymerase Chain Reaction, Gel Purification