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Agilent technologies bioanalyzer rna 6000 pico kit
Bioanalyzer Rna 6000 Pico Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bioanalyzer rna 6000 pico kit - by Bioz Stars, 2020-01
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Centrifugation:

Article Title: Polymerase pausing induced by sequence-specific RNA-binding protein drives heterochromatin assembly
Article Snippet: Following centrifugation at 14,000 rpm for 10 min at 4°C, the supernatant was transferred to a microcentrifuge tube, extracted once with chloroform, and precipitated with isopropanol. .. Input RNA quality and mRNA purity were verified by Bioanalyzer RNA 6000 Pico kit (Agilent, 5067-1513).

Article Title: Diguanylate Cyclases AdrA and STM1987 Regulate Salmonella enterica Exopolysaccharide Production during Plant Colonization in an Environment-Dependent Manner
Article Snippet: At 24 hpi, 10-ml samples containing planktonic bacteria from three petri dishes were combined (for 30 ml total per treatment) in cold Oakridge tubes containing 1/5 volume ice-cold stop solution (5% phenol in EtOH) and pelleted by centrifugation at 10,000 × g at 4°C. .. RNA quality was confirmed using Bio-Rad Experion RNA StdSens or Agilent Bioanalyzer RNA 6000 Pico kit protocols.

Amplification:

Article Title: Exosomes impact survival to radiation exposure in cell line models of nervous system cancer
Article Snippet: The extracted RNAs were quantified and quality checked using a BioAnalyzer RNA 6000 Pico Kit (Agilent Technologies). .. The synthesis of the second cDNA strand is performed by random priming, in a manner that DNA polymerase is efficiently stopped when reaching the next hybridized random primer, so only the fragment closest to the 3′ end gets captured for later indexed adapter ligation and PCR amplification.

Positive Control:

Article Title: Diet-induced obesity alters the maternal metabolome and early placenta transcriptome and decreases placenta vascularity in the mouse
Article Snippet: RNA quality was assessed using the Agilent Bioanalyzer RNA 6000 Pico Kit (Agilent) ensuring all RNA Integrity Numbers were > 8. .. Real-time quantitative reverse-transcription polymerase chain reaction (qPCR) was used on the RNA extracted from the corresponding epiblast to determine the sex of the embryo using TaqMan probes for inactive X specific transcripts ( Xist ) as a positive control (Mm01232884_m1) and eukaryotic translation initiation factor 2, subunit 3, structural gene Y-linked ( Eif2s3y ) (Mm01210630_m1) as a marker of male sex (Applied Biosystems).

Construct:

Article Title: Polymerase pausing induced by sequence-specific RNA-binding protein drives heterochromatin assembly
Article Snippet: Input RNA quality and mRNA purity were verified by Bioanalyzer RNA 6000 Pico kit (Agilent, 5067-1513). .. A sequencing library was constructed using NEBNext Ultra Directional RNA library preparation kit for Illumina (New England Biolab, E740S).

Article Title: Transcriptomic Analysis of the Campylobacter jejuni Response to T4-Like Phage NCTC 12673 Infection
Article Snippet: Successful rRNA depletion was confirmed using the Agilent Bioanalyzer RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA, USA). .. Strand-specific barcoded sequencing libraries were constructed using the Ion Total RNA-seq kit (Thermo Fisher Scientific, Waltham, MA, USA).

Real-time Polymerase Chain Reaction:

Article Title: Diguanylate Cyclases AdrA and STM1987 Regulate Salmonella enterica Exopolysaccharide Production during Plant Colonization in an Environment-Dependent Manner
Article Snippet: Real-time PCR was used to measure potential DNA contamination using 100 ng of RNA, SsoFast EvaGreen Supermix (Bio-Rad, Hercules, CA), and STM1987 primers ( ). .. RNA quality was confirmed using Bio-Rad Experion RNA StdSens or Agilent Bioanalyzer RNA 6000 Pico kit protocols.

Article Title: In Search for Reliable Markers of Glioma-Induced Polarization of Microglia
Article Snippet: The expression of selected genes were tested by qPCR with commercial primers for the following genes P2RY12, SIGLEC1, SELPLG, IFITM5, PTAFR, TNFRSF1B , and GPR84 , purchased from ScienCell Research Laboratories (sequences undisclosed). .. Samples were run on Bioanalyzer RNA 6000 Pico kit (Agilent, USA) to assess RNA integrity and concentration.

Article Title: Diet-induced obesity alters the maternal metabolome and early placenta transcriptome and decreases placenta vascularity in the mouse
Article Snippet: RNA quality was assessed using the Agilent Bioanalyzer RNA 6000 Pico Kit (Agilent) ensuring all RNA Integrity Numbers were > 8. .. Real-time quantitative reverse-transcription polymerase chain reaction (qPCR) was used on the RNA extracted from the corresponding epiblast to determine the sex of the embryo using TaqMan probes for inactive X specific transcripts ( Xist ) as a positive control (Mm01232884_m1) and eukaryotic translation initiation factor 2, subunit 3, structural gene Y-linked ( Eif2s3y ) (Mm01210630_m1) as a marker of male sex (Applied Biosystems).

Microarray:

Article Title: Diguanylate Cyclases AdrA and STM1987 Regulate Salmonella enterica Exopolysaccharide Production during Plant Colonization in an Environment-Dependent Manner
Article Snippet: RNA quality was confirmed using Bio-Rad Experion RNA StdSens or Agilent Bioanalyzer RNA 6000 Pico kit protocols. .. Total RNA was isolated from three biological replicates per strain for microarray analysis and from a second, independent set of three biological replicates per strain for real-time PCR confirmation of microarray results.

Incubation:

Article Title: Solid phase chemistry to covalently and reversibly capture thiolated RNA
Article Snippet: RNA was eluted with 10 μl elution buffer (100 mM NaCl, 10 mM Tris pH 7.4, 1 mM EDTA, 10 mM DTT and 100 pg fragmented total RNA from S. pombe (a generous gift from the Berro lab, Yale University) and incubated at room temperature in the dark for 15 min with rotation. .. Eluent was ethanol precipitated with 5–10 μg glycoblue, and RNA concentration was assayed by Bioanalyzer RNA 6000 Pico Kit (Agilent) according to the manufacturer's instructions.

Article Title: Diet-induced obesity alters the maternal metabolome and early placenta transcriptome and decreases placenta vascularity in the mouse
Article Snippet: The embryonic portion, or epiblast, was placed in 50 μl extraction buffer (Arcturus PicoPure RNA Isolation Kit), incubated at 42 ° F for 30 min, and then snap frozen and stored at –140 ° F. Similarly, the extraembryonic portion which includes the EPC and extra-embryonic ectoderm [ ] was separately processed in the same manner. .. RNA quality was assessed using the Agilent Bioanalyzer RNA 6000 Pico Kit (Agilent) ensuring all RNA Integrity Numbers were > 8.

Article Title: Genetic and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes
Article Snippet: RNA integrity number determined using the Bioanalyzer RNA 6000 Pico Kit (Agilent) for all samples was 10. .. Reverse transcription reactions were setup as follows: 5 μL RNA and 2 μL IgH isotype-specific primers (10 μM each) were heated to 72°C for 3 minutes then incubated at 42°C for 3 minutes to anneal primers; 13 μL of the following mixture was then added to each reaction for a final concentration of 1× First-strand buffer, 2 mM DTT, 1 μM 5′-Template Switch Adapter, 1 mM each dNTP (Invitrogen), 40 units RNaseOUT (Invitrogen) and 200 units SMARTscribe Reverse Transcriptase. cDNA was then treated with 5 units of Uracil DNA Glycosylase (New England Biolabs) to degrade residual template-switch adapter then purified using the NucleoSpin Gel and PCR Purification Kit (Takara).

Article Title: 3D Microfluidic Ex Vivo Culture of Organotypic Tumor Spheroids to Model Immune Checkpoint Blockade
Article Snippet: Devices were incubated for 25 min at 37°C, lysates were collected from each microfluidic chamber, and were transferred to RNase-free microcentrifuge tubes, and then stored at −80°C. .. RNA quantity and quality were assessed using Quant-iT™ RiboGreen™ RNA Assay Kit (Thermo Fisher, Cat. No. ) and Agilent Bioanalyzer RNA 6000 pico kit (Agilent, Cat. No. 5067–1513).

Stripping Membranes:

Article Title: In Search for Reliable Markers of Glioma-Induced Polarization of Microglia
Article Snippet: Global gene expression analysis using Affymetrix MG-430 PM Strip system (Affymetrix, USA) using GCM treated primary microglia for 6 h ( n = 3). .. Samples were run on Bioanalyzer RNA 6000 Pico kit (Agilent, USA) to assess RNA integrity and concentration.

Cell Culture:

Article Title: 3D Microfluidic Ex Vivo Culture of Organotypic Tumor Spheroids to Model Immune Checkpoint Blockade
Article Snippet: For RNA-seq studies, SI-NET PDOTS were cultured in 3D cell culture chips (AIM BIOTECH). .. RNA quantity and quality were assessed using Quant-iT™ RiboGreen™ RNA Assay Kit (Thermo Fisher, Cat. No. ) and Agilent Bioanalyzer RNA 6000 pico kit (Agilent, Cat. No. 5067–1513).

Expressing:

Article Title: In Search for Reliable Markers of Glioma-Induced Polarization of Microglia
Article Snippet: Paragraph title: Gene Expression Analysis ... Samples were run on Bioanalyzer RNA 6000 Pico kit (Agilent, USA) to assess RNA integrity and concentration.

Article Title: 3D Microfluidic Ex Vivo Culture of Organotypic Tumor Spheroids to Model Immune Checkpoint Blockade
Article Snippet: RNA quantity and quality were assessed using Quant-iT™ RiboGreen™ RNA Assay Kit (Thermo Fisher, Cat. No. ) and Agilent Bioanalyzer RNA 6000 pico kit (Agilent, Cat. No. 5067–1513). .. RNA-seq data were aligned and differential expression analysis were performed using VIPER pipleline, as described .

Hybridization:

Article Title: In Search for Reliable Markers of Glioma-Induced Polarization of Microglia
Article Snippet: Samples were run on Bioanalyzer RNA 6000 Pico kit (Agilent, USA) to assess RNA integrity and concentration. .. Hybridization was performed at 45°C for 16 h and fluidics, and imaging was done as described in the GeneAtlas System User’s Guide (P/N 08-0246).

Sequencing:

Article Title: Polymerase pausing induced by sequence-specific RNA-binding protein drives heterochromatin assembly
Article Snippet: Input RNA quality and mRNA purity were verified by Bioanalyzer RNA 6000 Pico kit (Agilent, 5067-1513). .. A sequencing library was constructed using NEBNext Ultra Directional RNA library preparation kit for Illumina (New England Biolab, E740S).

Article Title: Transcriptomic Analysis of the Campylobacter jejuni Response to T4-Like Phage NCTC 12673 Infection
Article Snippet: Successful rRNA depletion was confirmed using the Agilent Bioanalyzer RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA, USA). .. Strand-specific barcoded sequencing libraries were constructed using the Ion Total RNA-seq kit (Thermo Fisher Scientific, Waltham, MA, USA).

Article Title: Exosomes impact survival to radiation exposure in cell line models of nervous system cancer
Article Snippet: The extracted RNAs were quantified and quality checked using a BioAnalyzer RNA 6000 Pico Kit (Agilent Technologies). .. QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen) was used to generate mRNA-seq libraries as per manufacturer's recommendation, followed by deep sequencing on an Illumina HiSeq 2500 as per the manufacturer's instructions.

Article Title: Genetic and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes
Article Snippet: RNA integrity number determined using the Bioanalyzer RNA 6000 Pico Kit (Agilent) for all samples was 10. .. Total RNA was reverse transcribed using SMARTscribe Reverse Transcriptase (Takara) with IgH isotype-specific primers (1 μM each): IgHG1/2: 5′-CAGGGATCCAKAGTTC-3′, IgHG3: 5′-CAGGGCTCCATAGTTC-3′, IgHM: 5′-GATGACTTCAGTGTTGT-3′ and IgHD: 5′-AGTGGCTGACTTCCAA-3′; and 1μM template-switch adapter (5′-AAGCAGUGGTAUCAACGCAGAGUNNNNUNNNNUNNNNUCTTrGrGrGrG-3′, U=deoxyuridine) to introduce a unique molecular identifier (UMI) sequence to the 3′-end of each cDNA molecule.

Article Title: Impairments of spatial learning and memory following intrahippocampal injection in rats of 3-mercaptopropionic acid-modified CdTe quantum dots and molecular mechanisms
Article Snippet: Paragraph title: Transcriptome-library preparation and sequencing ... RNA integrity number was determined for each sample using a Bioanalyzer RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA, USA).

Article Title: Utilization of Tissue Ploidy Level Variation in de Novo Transcriptome Assembly of Pinus sylvestris
Article Snippet: Paragraph title: RNA isolation, library preparation and sequencing ... Fragment size distributions of mRNA, total RNA and libraries were verified with 2100 Bioanalyzer RNA 6000 Pico Kit and DNA 1000 kits (Agilent).

Ligation:

Article Title: Exosomes impact survival to radiation exposure in cell line models of nervous system cancer
Article Snippet: The extracted RNAs were quantified and quality checked using a BioAnalyzer RNA 6000 Pico Kit (Agilent Technologies). .. The synthesis of the second cDNA strand is performed by random priming, in a manner that DNA polymerase is efficiently stopped when reaching the next hybridized random primer, so only the fragment closest to the 3′ end gets captured for later indexed adapter ligation and PCR amplification.

Introduce:

Article Title: Genetic and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes
Article Snippet: RNA integrity number determined using the Bioanalyzer RNA 6000 Pico Kit (Agilent) for all samples was 10. .. Total RNA was reverse transcribed using SMARTscribe Reverse Transcriptase (Takara) with IgH isotype-specific primers (1 μM each): IgHG1/2: 5′-CAGGGATCCAKAGTTC-3′, IgHG3: 5′-CAGGGCTCCATAGTTC-3′, IgHM: 5′-GATGACTTCAGTGTTGT-3′ and IgHD: 5′-AGTGGCTGACTTCCAA-3′; and 1μM template-switch adapter (5′-AAGCAGUGGTAUCAACGCAGAGUNNNNUNNNNUNNNNUCTTrGrGrGrG-3′, U=deoxyuridine) to introduce a unique molecular identifier (UMI) sequence to the 3′-end of each cDNA molecule.

Generated:

Article Title: Transcriptomic Analysis of the Campylobacter jejuni Response to T4-Like Phage NCTC 12673 Infection
Article Snippet: RNA Sequencing Total mRNA libraries from all replicates were generated. .. Successful rRNA depletion was confirmed using the Agilent Bioanalyzer RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA, USA).

Imaging:

Article Title: In Search for Reliable Markers of Glioma-Induced Polarization of Microglia
Article Snippet: Samples were run on Bioanalyzer RNA 6000 Pico kit (Agilent, USA) to assess RNA integrity and concentration. .. Hybridization was performed at 45°C for 16 h and fluidics, and imaging was done as described in the GeneAtlas System User’s Guide (P/N 08-0246).

Polymerase Chain Reaction:

Article Title: Exosomes impact survival to radiation exposure in cell line models of nervous system cancer
Article Snippet: The extracted RNAs were quantified and quality checked using a BioAnalyzer RNA 6000 Pico Kit (Agilent Technologies). .. The synthesis of the second cDNA strand is performed by random priming, in a manner that DNA polymerase is efficiently stopped when reaching the next hybridized random primer, so only the fragment closest to the 3′ end gets captured for later indexed adapter ligation and PCR amplification.

Article Title: Genetic and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes
Article Snippet: RNA integrity number determined using the Bioanalyzer RNA 6000 Pico Kit (Agilent) for all samples was 10. .. Reverse transcription reactions were setup as follows: 5 μL RNA and 2 μL IgH isotype-specific primers (10 μM each) were heated to 72°C for 3 minutes then incubated at 42°C for 3 minutes to anneal primers; 13 μL of the following mixture was then added to each reaction for a final concentration of 1× First-strand buffer, 2 mM DTT, 1 μM 5′-Template Switch Adapter, 1 mM each dNTP (Invitrogen), 40 units RNaseOUT (Invitrogen) and 200 units SMARTscribe Reverse Transcriptase. cDNA was then treated with 5 units of Uracil DNA Glycosylase (New England Biolabs) to degrade residual template-switch adapter then purified using the NucleoSpin Gel and PCR Purification Kit (Takara).

Article Title: Utilization of Tissue Ploidy Level Variation in de Novo Transcriptome Assembly of Pinus sylvestris
Article Snippet: All libraries (6 trees × 5 tissues) were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs Inc.) with a fragmentation time of 5-12 min. An insert size selection of 300 bp was targeted using a concentration of 40-45 µl per 20 µl AMPure XP (Agencourt) and between 12-15 cycles of PCR during library preparation. .. Fragment size distributions of mRNA, total RNA and libraries were verified with 2100 Bioanalyzer RNA 6000 Pico Kit and DNA 1000 kits (Agilent).

Binding Assay:

Article Title: Solid phase chemistry to covalently and reversibly capture thiolated RNA
Article Snippet: Beads were incubated for 5 min with the following wash buffers: once with 100 μl 1× binding buffer, twice with 100 μl high salt wash buffer (1 M NaCl, 100 mM Tris pH 7.4, 10 mM EDTA, 0.05% Tween), twice with 100 μl denaturing buffer (8 M guanidinium chloride) and three times with 100 μl buffer TE (10 mM Tris pH 7.4, 1 mM EDTA) at 55°C. .. Eluent was ethanol precipitated with 5–10 μg glycoblue, and RNA concentration was assayed by Bioanalyzer RNA 6000 Pico Kit (Agilent) according to the manufacturer's instructions.

RNA Sequencing Assay:

Article Title: Polymerase pausing induced by sequence-specific RNA-binding protein drives heterochromatin assembly
Article Snippet: Paragraph title: RNA-seq ... Input RNA quality and mRNA purity were verified by Bioanalyzer RNA 6000 Pico kit (Agilent, 5067-1513).

Article Title: Transcriptomic Analysis of the Campylobacter jejuni Response to T4-Like Phage NCTC 12673 Infection
Article Snippet: Paragraph title: 2.4. RNA Sequencing ... Successful rRNA depletion was confirmed using the Agilent Bioanalyzer RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA, USA).

Article Title: Diet-induced obesity alters the maternal metabolome and early placenta transcriptome and decreases placenta vascularity in the mouse
Article Snippet: RNA quality was assessed using the Agilent Bioanalyzer RNA 6000 Pico Kit (Agilent) ensuring all RNA Integrity Numbers were > 8. .. RNA-Seq libraries were prepared on individual sexed EPC (n = 5 for female embryos and n = 4 for male embryos from each diet group) using the SMARTer Stranded RNA-Seq kit (Clontech).

Article Title: Transcriptome analysis of Pseudomonas aeruginosa PAO1 grown at both body and elevated temperatures
Article Snippet: The quality of the extracted RNA samples was also determined using an Agilent Bioanalyzer-RNA 6000 Pico Kit (Agilent Technologies, CA, USA). .. Synthesis of cDNA was performed using the protocols of ScriptSeq™ v2 RNA-seq Library Preparation Kit (Epicentre, WI, USA).

Article Title: 3D Microfluidic Ex Vivo Culture of Organotypic Tumor Spheroids to Model Immune Checkpoint Blockade
Article Snippet: Paragraph title: RNA-sequencing (RNA-seq) and CIBERSORT. ... RNA quantity and quality were assessed using Quant-iT™ RiboGreen™ RNA Assay Kit (Thermo Fisher, Cat. No. ) and Agilent Bioanalyzer RNA 6000 pico kit (Agilent, Cat. No. 5067–1513).

RNA HS Assay:

Article Title: Utilization of Tissue Ploidy Level Variation in de Novo Transcriptome Assembly of Pinus sylvestris
Article Snippet: RNA concentration was quantified using Qubit 2.0 (Invitrogen) and Qubit RNA HS Assay Kit (Thermo Fisher Scientific). .. Fragment size distributions of mRNA, total RNA and libraries were verified with 2100 Bioanalyzer RNA 6000 Pico Kit and DNA 1000 kits (Agilent).

Isolation:

Article Title: Diguanylate Cyclases AdrA and STM1987 Regulate Salmonella enterica Exopolysaccharide Production during Plant Colonization in an Environment-Dependent Manner
Article Snippet: Paragraph title: RNA isolation. ... RNA quality was confirmed using Bio-Rad Experion RNA StdSens or Agilent Bioanalyzer RNA 6000 Pico kit protocols.

Article Title: Exosomes impact survival to radiation exposure in cell line models of nervous system cancer
Article Snippet: Exosomes from flasks were isolated and run for RNA exploration analysis. .. The extracted RNAs were quantified and quality checked using a BioAnalyzer RNA 6000 Pico Kit (Agilent Technologies).

Article Title: Diet-induced obesity alters the maternal metabolome and early placenta transcriptome and decreases placenta vascularity in the mouse
Article Snippet: Total RNA was isolated from tissues using the Arcturus PicoPure RNA Isolation Kit (Thermo Fisher), including an on-column DNase digestion (Qiagen). .. RNA quality was assessed using the Agilent Bioanalyzer RNA 6000 Pico Kit (Agilent) ensuring all RNA Integrity Numbers were > 8.

Article Title: Utilization of Tissue Ploidy Level Variation in de Novo Transcriptome Assembly of Pinus sylvestris
Article Snippet: Paragraph title: RNA isolation, library preparation and sequencing ... Fragment size distributions of mRNA, total RNA and libraries were verified with 2100 Bioanalyzer RNA 6000 Pico Kit and DNA 1000 kits (Agilent).

Size-exclusion Chromatography:

Article Title: Polymerase pausing induced by sequence-specific RNA-binding protein drives heterochromatin assembly
Article Snippet: Zirconia-silica beads (0.5-mm; BioSpec, 11079105z) were added, and lysis was accomplished by three cycles of bead beating for 90 sec on high (Bead Ruptor 12 homogenizer, OMNI International). .. Input RNA quality and mRNA purity were verified by Bioanalyzer RNA 6000 Pico kit (Agilent, 5067-1513).

Microscopy:

Article Title: Diet-induced obesity alters the maternal metabolome and early placenta transcriptome and decreases placenta vascularity in the mouse
Article Snippet: The decidua was freed from the muscular uterine lining, and the embryo was shelled out from the decidua using forceps under a dissecting microscope. .. RNA quality was assessed using the Agilent Bioanalyzer RNA 6000 Pico Kit (Agilent) ensuring all RNA Integrity Numbers were > 8.

Purification:

Article Title: Solid phase chemistry to covalently and reversibly capture thiolated RNA
Article Snippet: Paragraph title: Purification of s4 U-RNA with MTS-resin ... Eluent was ethanol precipitated with 5–10 μg glycoblue, and RNA concentration was assayed by Bioanalyzer RNA 6000 Pico Kit (Agilent) according to the manufacturer's instructions.

Article Title: Genetic and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes
Article Snippet: Total RNA was purified using RNeasy Micro Kit (Qiagen). .. RNA integrity number determined using the Bioanalyzer RNA 6000 Pico Kit (Agilent) for all samples was 10.

Article Title: Transcriptome analysis of Pseudomonas aeruginosa PAO1 grown at both body and elevated temperatures
Article Snippet: RNA extraction and cDNA synthesis Total RNA was extracted using MasterPure™ RNA Purification Kit (Epicentre, WI, USA) as per the manufacturer’s instructions. .. The quality of the extracted RNA samples was also determined using an Agilent Bioanalyzer-RNA 6000 Pico Kit (Agilent Technologies, CA, USA).

Reverse Transcription Polymerase Chain Reaction:

Article Title: Diet-induced obesity alters the maternal metabolome and early placenta transcriptome and decreases placenta vascularity in the mouse
Article Snippet: RNA quality was assessed using the Agilent Bioanalyzer RNA 6000 Pico Kit (Agilent) ensuring all RNA Integrity Numbers were > 8. .. Real-time quantitative reverse-transcription polymerase chain reaction (qPCR) was used on the RNA extracted from the corresponding epiblast to determine the sex of the embryo using TaqMan probes for inactive X specific transcripts ( Xist ) as a positive control (Mm01232884_m1) and eukaryotic translation initiation factor 2, subunit 3, structural gene Y-linked ( Eif2s3y ) (Mm01210630_m1) as a marker of male sex (Applied Biosystems).

Chloramphenicol Acetyltransferase Assay:

Article Title: Exosomes impact survival to radiation exposure in cell line models of nervous system cancer
Article Snippet: Total exosomal RNA was extracted using mirVana kit (Ambion, cat:AM1560). .. The extracted RNAs were quantified and quality checked using a BioAnalyzer RNA 6000 Pico Kit (Agilent Technologies).

Mouse Assay:

Article Title: Genetic and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes
Article Snippet: Aicda-/- mice served as controls. .. RNA integrity number determined using the Bioanalyzer RNA 6000 Pico Kit (Agilent) for all samples was 10.

Chromatin Immunoprecipitation:

Article Title: Transcriptomic Analysis of the Campylobacter jejuni Response to T4-Like Phage NCTC 12673 Infection
Article Snippet: Successful rRNA depletion was confirmed using the Agilent Bioanalyzer RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA, USA). .. The pooled libraries were templated using the Ion PI Hi-Q kit (Thermo Fisher Scientific, Waltham, MA, USA) and sequenced on an Ion Torrent Proton using the Ion PI Hi-Q sequencing 200 kit (Thermo Fisher Scientific, Waltham, MA, USA) on a single Proton V2 chip.

RNA Extraction:

Article Title: Transcriptome analysis of Pseudomonas aeruginosa PAO1 grown at both body and elevated temperatures
Article Snippet: Paragraph title: RNA extraction and cDNA synthesis ... The quality of the extracted RNA samples was also determined using an Agilent Bioanalyzer-RNA 6000 Pico Kit (Agilent Technologies, CA, USA).

Article Title: Utilization of Tissue Ploidy Level Variation in de Novo Transcriptome Assembly of Pinus sylvestris
Article Snippet: After total RNA extraction, mRNA was captured using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs Inc.). mRNA extractions were treated with Turbo DNA-free Kit (Thermo Fisher Scientific). .. Fragment size distributions of mRNA, total RNA and libraries were verified with 2100 Bioanalyzer RNA 6000 Pico Kit and DNA 1000 kits (Agilent).

Software:

Article Title: In Search for Reliable Markers of Glioma-Induced Polarization of Microglia
Article Snippet: Samples were run on Bioanalyzer RNA 6000 Pico kit (Agilent, USA) to assess RNA integrity and concentration. .. Data were analyzed using R Studio software and Bioconductor package, and data were deposited into GEO Repository ( ).

Multiplex Assay:

Article Title: Utilization of Tissue Ploidy Level Variation in de Novo Transcriptome Assembly of Pinus sylvestris
Article Snippet: Libraries were indexed using NEBNext Multiplex Oligos for Illumina, Single Index Set 1. .. Fragment size distributions of mRNA, total RNA and libraries were verified with 2100 Bioanalyzer RNA 6000 Pico Kit and DNA 1000 kits (Agilent).

Selection:

Article Title: Utilization of Tissue Ploidy Level Variation in de Novo Transcriptome Assembly of Pinus sylvestris
Article Snippet: All libraries (6 trees × 5 tissues) were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs Inc.) with a fragmentation time of 5-12 min. An insert size selection of 300 bp was targeted using a concentration of 40-45 µl per 20 µl AMPure XP (Agencourt) and between 12-15 cycles of PCR during library preparation. .. Fragment size distributions of mRNA, total RNA and libraries were verified with 2100 Bioanalyzer RNA 6000 Pico Kit and DNA 1000 kits (Agilent).

Sample Prep:

Article Title: Impairments of spatial learning and memory following intrahippocampal injection in rats of 3-mercaptopropionic acid-modified CdTe quantum dots and molecular mechanisms
Article Snippet: RNA integrity number was determined for each sample using a Bioanalyzer RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA, USA). .. The complementary cDNA libraries were prepared using the TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA, USA) as per the manufacturer’s instructions.

Spectrophotometry:

Article Title: Transcriptome analysis of Pseudomonas aeruginosa PAO1 grown at both body and elevated temperatures
Article Snippet: Purity of RNA samples was assessed using a NanoDrop 2000 Spectrophotometer (Thermo Scientific, MA, USA) and only samples with A260 /A280 and A260 /A230 values > 2.0 were chosen for further work. .. The quality of the extracted RNA samples was also determined using an Agilent Bioanalyzer-RNA 6000 Pico Kit (Agilent Technologies, CA, USA).

Concentration Assay:

Article Title: Solid phase chemistry to covalently and reversibly capture thiolated RNA
Article Snippet: .. Eluent was ethanol precipitated with 5–10 μg glycoblue, and RNA concentration was assayed by Bioanalyzer RNA 6000 Pico Kit (Agilent) according to the manufacturer's instructions. .. MTS-resin binding capacity A solution of 100 nmol s4 U nucleoside in 18 μl RNase-free water was mixed with 3 μl 10× binding buffer and 6 μl DMF (total volume 30 μl) was enriched using 1, 2, 5, 10 or 20 μl MTS-resin as above.

Article Title: In Search for Reliable Markers of Glioma-Induced Polarization of Microglia
Article Snippet: .. Samples were run on Bioanalyzer RNA 6000 Pico kit (Agilent, USA) to assess RNA integrity and concentration. ..

Article Title: Genetic and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes
Article Snippet: RNA integrity number determined using the Bioanalyzer RNA 6000 Pico Kit (Agilent) for all samples was 10. .. Reverse transcription reactions were setup as follows: 5 μL RNA and 2 μL IgH isotype-specific primers (10 μM each) were heated to 72°C for 3 minutes then incubated at 42°C for 3 minutes to anneal primers; 13 μL of the following mixture was then added to each reaction for a final concentration of 1× First-strand buffer, 2 mM DTT, 1 μM 5′-Template Switch Adapter, 1 mM each dNTP (Invitrogen), 40 units RNaseOUT (Invitrogen) and 200 units SMARTscribe Reverse Transcriptase. cDNA was then treated with 5 units of Uracil DNA Glycosylase (New England Biolabs) to degrade residual template-switch adapter then purified using the NucleoSpin Gel and PCR Purification Kit (Takara).

Article Title: Utilization of Tissue Ploidy Level Variation in de Novo Transcriptome Assembly of Pinus sylvestris
Article Snippet: All libraries (6 trees × 5 tissues) were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs Inc.) with a fragmentation time of 5-12 min. An insert size selection of 300 bp was targeted using a concentration of 40-45 µl per 20 µl AMPure XP (Agencourt) and between 12-15 cycles of PCR during library preparation. .. Fragment size distributions of mRNA, total RNA and libraries were verified with 2100 Bioanalyzer RNA 6000 Pico Kit and DNA 1000 kits (Agilent).

Marker:

Article Title: Diet-induced obesity alters the maternal metabolome and early placenta transcriptome and decreases placenta vascularity in the mouse
Article Snippet: RNA quality was assessed using the Agilent Bioanalyzer RNA 6000 Pico Kit (Agilent) ensuring all RNA Integrity Numbers were > 8. .. Real-time quantitative reverse-transcription polymerase chain reaction (qPCR) was used on the RNA extracted from the corresponding epiblast to determine the sex of the embryo using TaqMan probes for inactive X specific transcripts ( Xist ) as a positive control (Mm01232884_m1) and eukaryotic translation initiation factor 2, subunit 3, structural gene Y-linked ( Eif2s3y ) (Mm01210630_m1) as a marker of male sex (Applied Biosystems).

Lysis:

Article Title: Polymerase pausing induced by sequence-specific RNA-binding protein drives heterochromatin assembly
Article Snippet: Zirconia-silica beads (0.5-mm; BioSpec, 11079105z) were added, and lysis was accomplished by three cycles of bead beating for 90 sec on high (Bead Ruptor 12 homogenizer, OMNI International). .. Input RNA quality and mRNA purity were verified by Bioanalyzer RNA 6000 Pico kit (Agilent, 5067-1513).

Article Title: 3D Microfluidic Ex Vivo Culture of Organotypic Tumor Spheroids to Model Immune Checkpoint Blockade
Article Snippet: Conditioned media was removed (as described above), before 200 mL of lysis buffer (with proteinase K) was added to each microfluidic chamber. .. RNA quantity and quality were assessed using Quant-iT™ RiboGreen™ RNA Assay Kit (Thermo Fisher, Cat. No. ) and Agilent Bioanalyzer RNA 6000 pico kit (Agilent, Cat. No. 5067–1513).

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  • 99
    Agilent technologies rna 6000 pico kit
    Characterization of exosomal RNA (esRNA) extracted from purified nanovesicles from fresh human aqueous humor. The esRNA was evaluated using Agilent Bioanalyzer 2100 with Agilent <t>RNA</t> 6000 Pico Kit. A) Shows the RNA ladder and B) displays extracted esRNA from one of the human aqueous humor samples. The size of extracted esRNA was less than 200 nucleotides (nt), mostly around 25 nt. C) shows the miRNAs that were identified via small RNA sequencing using Illumina MiSeq sequencing system. The height of each bar indicated the number of sequence reads with perfect match to mature miRNA.
    Rna 6000 Pico Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 200 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna 6000 pico kit/product/Agilent technologies
    Average 99 stars, based on 200 article reviews
    Price from $9.99 to $1999.99
    rna 6000 pico kit - by Bioz Stars, 2020-01
    99/100 stars
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    79
    Agilent technologies 6000 rna pico bioanalyzer kit
    Determination of the correct injection site and quality assessment of <t>RNA</t> extracted from swatches. ( A ) Correct injection site was determined using india ink (left panel; black: india ink, red: Nissl stain) and lentiviral vectors carrying tGFP (middle: unfixed tissue and right: fixed tissue panels; green fluorescence: tGFP-positive transduced neurons; red: fluorescent Nissl stain [NeuroTrace Fluorescent Nissl Stain # N-21482, Molecular Probes]). ( B ) Assessment of RNA quality in microdissected SO-CA3/2 of tGFP-expressing neurons. Extracted RNA was assessed using an Agilent <t>Bioanalyzer.</t> The sharp peaks at 40 and 47 s depict intact 18s and 28s rRNA, respectively (upper and lower left panels). Panels on the right show typical peaks of amplified RNA (aRNA), ensuring the high quality of the extracted RNA.
    6000 Rna Pico Bioanalyzer Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/6000 rna pico bioanalyzer kit/product/Agilent technologies
    Average 79 stars, based on 1 article reviews
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    6000 rna pico bioanalyzer kit - by Bioz Stars, 2020-01
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    96
    Agilent technologies agilent bioanalyzer analysis
    (A) <t>Agilent</t> <t>Bioanalyzer</t> analysis (High-sensitivity DNA Kit) of a SPRI-beads-purified DNA library is shown. DNA fragments smaller than 175 bp include library DNAs with very short cDNA inserts, PCR primer dimers, and residual PCR primers. Gel purification
    Agilent Bioanalyzer Analysis, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 96/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Characterization of exosomal RNA (esRNA) extracted from purified nanovesicles from fresh human aqueous humor. The esRNA was evaluated using Agilent Bioanalyzer 2100 with Agilent RNA 6000 Pico Kit. A) Shows the RNA ladder and B) displays extracted esRNA from one of the human aqueous humor samples. The size of extracted esRNA was less than 200 nucleotides (nt), mostly around 25 nt. C) shows the miRNAs that were identified via small RNA sequencing using Illumina MiSeq sequencing system. The height of each bar indicated the number of sequence reads with perfect match to mature miRNA.

    Journal: Experimental eye research

    Article Title: Human Aqueous Humor Exosomes

    doi: 10.1016/j.exer.2015.01.019

    Figure Lengend Snippet: Characterization of exosomal RNA (esRNA) extracted from purified nanovesicles from fresh human aqueous humor. The esRNA was evaluated using Agilent Bioanalyzer 2100 with Agilent RNA 6000 Pico Kit. A) Shows the RNA ladder and B) displays extracted esRNA from one of the human aqueous humor samples. The size of extracted esRNA was less than 200 nucleotides (nt), mostly around 25 nt. C) shows the miRNAs that were identified via small RNA sequencing using Illumina MiSeq sequencing system. The height of each bar indicated the number of sequence reads with perfect match to mature miRNA.

    Article Snippet: Quality of extracted esRNA was evaluated using Agilent Bioanalyzer 2100 with Agilent RNA 6000 Pico Kit.

    Techniques: Purification, RNA Sequencing Assay, Sequencing

    Determination of the correct injection site and quality assessment of RNA extracted from swatches. ( A ) Correct injection site was determined using india ink (left panel; black: india ink, red: Nissl stain) and lentiviral vectors carrying tGFP (middle: unfixed tissue and right: fixed tissue panels; green fluorescence: tGFP-positive transduced neurons; red: fluorescent Nissl stain [NeuroTrace Fluorescent Nissl Stain # N-21482, Molecular Probes]). ( B ) Assessment of RNA quality in microdissected SO-CA3/2 of tGFP-expressing neurons. Extracted RNA was assessed using an Agilent Bioanalyzer. The sharp peaks at 40 and 47 s depict intact 18s and 28s rRNA, respectively (upper and lower left panels). Panels on the right show typical peaks of amplified RNA (aRNA), ensuring the high quality of the extracted RNA.

    Journal: Cerebral Cortex (New York, NY)

    Article Title: Epigenetic Regulation of Glutamic Acid Decarboxylase 67 in a Hippocampal Circuit

    doi: 10.1093/cercor/bhw307

    Figure Lengend Snippet: Determination of the correct injection site and quality assessment of RNA extracted from swatches. ( A ) Correct injection site was determined using india ink (left panel; black: india ink, red: Nissl stain) and lentiviral vectors carrying tGFP (middle: unfixed tissue and right: fixed tissue panels; green fluorescence: tGFP-positive transduced neurons; red: fluorescent Nissl stain [NeuroTrace Fluorescent Nissl Stain # N-21482, Molecular Probes]). ( B ) Assessment of RNA quality in microdissected SO-CA3/2 of tGFP-expressing neurons. Extracted RNA was assessed using an Agilent Bioanalyzer. The sharp peaks at 40 and 47 s depict intact 18s and 28s rRNA, respectively (upper and lower left panels). Panels on the right show typical peaks of amplified RNA (aRNA), ensuring the high quality of the extracted RNA.

    Article Snippet: This process consisted of passing the slides through RNAse free-graded ethanol solutions (50%, 75%, 95%, and 100% at 4°C, 100% at RT) for 15 s each followed by drying the slide at 37°C for 5 min. RNA extracted from tissue from the 1% PFA brain was checked for quality using the Agilent 6000 RNA Pico Bioanalyzer Kit.

    Techniques: Injection, Staining, Fluorescence, Expressing, Amplification

    (A) Agilent Bioanalyzer analysis (High-sensitivity DNA Kit) of a SPRI-beads-purified DNA library is shown. DNA fragments smaller than 175 bp include library DNAs with very short cDNA inserts, PCR primer dimers, and residual PCR primers. Gel purification

    Journal: Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]

    Article Title: Current Protocols in Molecular Biology Unit 4.23

    doi: 10.1002/0471142727.mb0423s110

    Figure Lengend Snippet: (A) Agilent Bioanalyzer analysis (High-sensitivity DNA Kit) of a SPRI-beads-purified DNA library is shown. DNA fragments smaller than 175 bp include library DNAs with very short cDNA inserts, PCR primer dimers, and residual PCR primers. Gel purification

    Article Snippet: The size distribution of the RNA fragments can be examined by Agilent Bioanalyzer analysis (RNA 6000 Pico Kit).

    Techniques: Purification, Polymerase Chain Reaction, Gel Purification