Structured Review

Agilent technologies bioanalyzer 2100
Example Agilent <t>Bioanalyzer</t> 2100 trace of 3'-seq library. High quality library should result in a size range distribution as expected from the size range extracted from the gel. Shown is a 3'-seq library that falls in the 150–250 bp size range (labeled). Isolation of a fraction of reads below 175bp is to be expected and will still results in reads that can be mapped to the genome. The peak should be above 130 bp to avoid contamination of adapter-adapter reads (~120 bp). On the right side the densitometry plot with the standard and the sample are shown. On the left an electropherogram of the sample is plotted with fluorescent intensity (FU) on the red y axis and size (nt) on the×axis. The markers run together with the sample are labeled. STD- standard.
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1) Product Images from "Genome-wide profiling of the 3' ends of polyadenylated RNAs"

Article Title: Genome-wide profiling of the 3' ends of polyadenylated RNAs

Journal: Methods (San Diego, Calif.)

doi: 10.1016/j.ymeth.2017.06.003

Example Agilent Bioanalyzer 2100 trace of 3'-seq library. High quality library should result in a size range distribution as expected from the size range extracted from the gel. Shown is a 3'-seq library that falls in the 150–250 bp size range (labeled). Isolation of a fraction of reads below 175bp is to be expected and will still results in reads that can be mapped to the genome. The peak should be above 130 bp to avoid contamination of adapter-adapter reads (~120 bp). On the right side the densitometry plot with the standard and the sample are shown. On the left an electropherogram of the sample is plotted with fluorescent intensity (FU) on the red y axis and size (nt) on the×axis. The markers run together with the sample are labeled. STD- standard.
Figure Legend Snippet: Example Agilent Bioanalyzer 2100 trace of 3'-seq library. High quality library should result in a size range distribution as expected from the size range extracted from the gel. Shown is a 3'-seq library that falls in the 150–250 bp size range (labeled). Isolation of a fraction of reads below 175bp is to be expected and will still results in reads that can be mapped to the genome. The peak should be above 130 bp to avoid contamination of adapter-adapter reads (~120 bp). On the right side the densitometry plot with the standard and the sample are shown. On the left an electropherogram of the sample is plotted with fluorescent intensity (FU) on the red y axis and size (nt) on the×axis. The markers run together with the sample are labeled. STD- standard.

Techniques Used: Labeling, Isolation

Example of high quality total RNA. Total RNA quality is assessed on the basis of the quality of the predominant signal from rRNA (as labeled). The trace is from high quality total RNA from  Drosophila melanogaster  run on an Agilent Bioanalyzer 2100. Notice that insect 28S rRNA dissociates into two subunits of equal size that co- migrate with the 18S rRNA. The migration of rRNA of other organisms will vary and should be taken into account when validating total RNA quality. Bioanalyzer output is shown. On the right the densitometry plot with the standard and the sample are shown. On the left an electropherogram of the sample is plotted with fluorescent intensity (FU) on the red y axis and size (nt) on the×axis. The marker run together with the sample is labeled. STD- standard.
Figure Legend Snippet: Example of high quality total RNA. Total RNA quality is assessed on the basis of the quality of the predominant signal from rRNA (as labeled). The trace is from high quality total RNA from Drosophila melanogaster run on an Agilent Bioanalyzer 2100. Notice that insect 28S rRNA dissociates into two subunits of equal size that co- migrate with the 18S rRNA. The migration of rRNA of other organisms will vary and should be taken into account when validating total RNA quality. Bioanalyzer output is shown. On the right the densitometry plot with the standard and the sample are shown. On the left an electropherogram of the sample is plotted with fluorescent intensity (FU) on the red y axis and size (nt) on the×axis. The marker run together with the sample is labeled. STD- standard.

Techniques Used: Labeling, Migration, Marker

Optimization of fragmentation time. Agilent Bioanalyzer 2100 traces of the reaction outlined in 2.2.3 stopped at different time intervals. The chemical fragmentation reaction should be stopped when the total RNA peak is around 150–200 nt (10 min) but before the RNA is completely fragmented as shown in the later time points. This step was optimized for fragmentation of Drosophila total RNA and should be optimized when using this protocol to determine 3' ends from total RNA of other organisms. Densitometry of the samples run is shown to the left while electropherograms are shown on the right. Samples are labeled above each lane and on each corresponding inset on the right. The marker, present in each sample, is labeled in the top most panel. STD- standard. FU - fluorescent intensity.
Figure Legend Snippet: Optimization of fragmentation time. Agilent Bioanalyzer 2100 traces of the reaction outlined in 2.2.3 stopped at different time intervals. The chemical fragmentation reaction should be stopped when the total RNA peak is around 150–200 nt (10 min) but before the RNA is completely fragmented as shown in the later time points. This step was optimized for fragmentation of Drosophila total RNA and should be optimized when using this protocol to determine 3' ends from total RNA of other organisms. Densitometry of the samples run is shown to the left while electropherograms are shown on the right. Samples are labeled above each lane and on each corresponding inset on the right. The marker, present in each sample, is labeled in the top most panel. STD- standard. FU - fluorescent intensity.

Techniques Used: Labeling, Marker

2) Product Images from "Hemoprotein Bach1 regulates enhancer availability of heme oxygenase-1 gene"

Article Title: Hemoprotein Bach1 regulates enhancer availability of heme oxygenase-1 gene

Journal: The EMBO Journal

doi: 10.1093/emboj/cdf516

Fig. 6.  Binding of small Maf to  ho-1  enhancers  in vivo . ( A ) Gel images showing PCR amplification of E2, exon 1 and Rag-2 gene fragments recovered in the absence or presence of anti-small Maf antibodies. Chromatin was prepared from the wild-type (WT) or  bach1 -deficient (KO) thymi. PCR products without DNA template are shown in the right-hand lanes. ( B ) Fold-enrichment of each genomic DNA region by the small Maf antibodies was determined using the Agilent BioAnalyzer 2100. Results are mean of two experiments using two mice per each genotype. ( C ) A hypothetical model describing the regulation of  ho-1  by Bach1 and heme. Besides MafK, other Maf-related factors may also serve as partners for Bach1. Bach1 makes enhancers inaccessible to Nrf2 and other activators by binding to them. Repression by Bach1 is alleviated upon increase of heme levels, allowing expression of HO-1 followed by degradation of heme.
Figure Legend Snippet: Fig. 6. Binding of small Maf to ho-1 enhancers in vivo . ( A ) Gel images showing PCR amplification of E2, exon 1 and Rag-2 gene fragments recovered in the absence or presence of anti-small Maf antibodies. Chromatin was prepared from the wild-type (WT) or bach1 -deficient (KO) thymi. PCR products without DNA template are shown in the right-hand lanes. ( B ) Fold-enrichment of each genomic DNA region by the small Maf antibodies was determined using the Agilent BioAnalyzer 2100. Results are mean of two experiments using two mice per each genotype. ( C ) A hypothetical model describing the regulation of ho-1 by Bach1 and heme. Besides MafK, other Maf-related factors may also serve as partners for Bach1. Bach1 makes enhancers inaccessible to Nrf2 and other activators by binding to them. Repression by Bach1 is alleviated upon increase of heme levels, allowing expression of HO-1 followed by degradation of heme.

Techniques Used: Binding Assay, In Vivo, Polymerase Chain Reaction, Amplification, Mouse Assay, Expressing

Fig. 4.  Expression of HO-1 in  bach1 -deficient mice. ( A ) Protein extracts of indicated tissues of  bach1 +/+  or  bach1 –/–  (shown by W and K, respectively) mice were analyzed for expression of HO-1 by immunoblot assays (upper panels). HO-1 is indicated by an arrowhead. Membranes were stained for protein with amido black to verify equal loading (lower panels). ( B ) HO-1 and HPRT mRNA levels in thymus, heart, lung and liver from various genotypes (indicated above the panels) were compared by RT–PCR. To ensure linearity of amplification, 3-fold dilutions of cDNA (lanes 1–3 and 4–6, and indicated with H and L at the top) were used as templates. Images were recorded using the Agilent BioAnalyzer 2100.
Figure Legend Snippet: Fig. 4. Expression of HO-1 in bach1 -deficient mice. ( A ) Protein extracts of indicated tissues of bach1 +/+ or bach1 –/– (shown by W and K, respectively) mice were analyzed for expression of HO-1 by immunoblot assays (upper panels). HO-1 is indicated by an arrowhead. Membranes were stained for protein with amido black to verify equal loading (lower panels). ( B ) HO-1 and HPRT mRNA levels in thymus, heart, lung and liver from various genotypes (indicated above the panels) were compared by RT–PCR. To ensure linearity of amplification, 3-fold dilutions of cDNA (lanes 1–3 and 4–6, and indicated with H and L at the top) were used as templates. Images were recorded using the Agilent BioAnalyzer 2100.

Techniques Used: Expressing, Mouse Assay, Staining, Reverse Transcription Polymerase Chain Reaction, Amplification

3) Product Images from "Hemoprotein Bach1 regulates enhancer availability of heme oxygenase-1 gene"

Article Title: Hemoprotein Bach1 regulates enhancer availability of heme oxygenase-1 gene

Journal: The EMBO Journal

doi: 10.1093/emboj/cdf516

Fig. 6.  Binding of small Maf to  ho-1  enhancers  in vivo . ( A ) Gel images showing PCR amplification of E2, exon 1 and Rag-2 gene fragments recovered in the absence or presence of anti-small Maf antibodies. Chromatin was prepared from the wild-type (WT) or  bach1 -deficient (KO) thymi. PCR products without DNA template are shown in the right-hand lanes. ( B ) Fold-enrichment of each genomic DNA region by the small Maf antibodies was determined using the Agilent BioAnalyzer 2100. Results are mean of two experiments using two mice per each genotype. ( C ) A hypothetical model describing the regulation of  ho-1  by Bach1 and heme. Besides MafK, other Maf-related factors may also serve as partners for Bach1. Bach1 makes enhancers inaccessible to Nrf2 and other activators by binding to them. Repression by Bach1 is alleviated upon increase of heme levels, allowing expression of HO-1 followed by degradation of heme.
Figure Legend Snippet: Fig. 6. Binding of small Maf to ho-1 enhancers in vivo . ( A ) Gel images showing PCR amplification of E2, exon 1 and Rag-2 gene fragments recovered in the absence or presence of anti-small Maf antibodies. Chromatin was prepared from the wild-type (WT) or bach1 -deficient (KO) thymi. PCR products without DNA template are shown in the right-hand lanes. ( B ) Fold-enrichment of each genomic DNA region by the small Maf antibodies was determined using the Agilent BioAnalyzer 2100. Results are mean of two experiments using two mice per each genotype. ( C ) A hypothetical model describing the regulation of ho-1 by Bach1 and heme. Besides MafK, other Maf-related factors may also serve as partners for Bach1. Bach1 makes enhancers inaccessible to Nrf2 and other activators by binding to them. Repression by Bach1 is alleviated upon increase of heme levels, allowing expression of HO-1 followed by degradation of heme.

Techniques Used: Binding Assay, In Vivo, Polymerase Chain Reaction, Amplification, Mouse Assay, Expressing

Fig. 4.  Expression of HO-1 in  bach1 -deficient mice. ( A ) Protein extracts of indicated tissues of  bach1 +/+  or  bach1 –/–  (shown by W and K, respectively) mice were analyzed for expression of HO-1 by immunoblot assays (upper panels). HO-1 is indicated by an arrowhead. Membranes were stained for protein with amido black to verify equal loading (lower panels). ( B ) HO-1 and HPRT mRNA levels in thymus, heart, lung and liver from various genotypes (indicated above the panels) were compared by RT–PCR. To ensure linearity of amplification, 3-fold dilutions of cDNA (lanes 1–3 and 4–6, and indicated with H and L at the top) were used as templates. Images were recorded using the Agilent BioAnalyzer 2100.
Figure Legend Snippet: Fig. 4. Expression of HO-1 in bach1 -deficient mice. ( A ) Protein extracts of indicated tissues of bach1 +/+ or bach1 –/– (shown by W and K, respectively) mice were analyzed for expression of HO-1 by immunoblot assays (upper panels). HO-1 is indicated by an arrowhead. Membranes were stained for protein with amido black to verify equal loading (lower panels). ( B ) HO-1 and HPRT mRNA levels in thymus, heart, lung and liver from various genotypes (indicated above the panels) were compared by RT–PCR. To ensure linearity of amplification, 3-fold dilutions of cDNA (lanes 1–3 and 4–6, and indicated with H and L at the top) were used as templates. Images were recorded using the Agilent BioAnalyzer 2100.

Techniques Used: Expressing, Mouse Assay, Staining, Reverse Transcription Polymerase Chain Reaction, Amplification

4) Product Images from "Time-Dependent Decay of mRNA and Ribosomal RNA during Platelet Aging and Its Correlation with Translation Activity"

Article Title: Time-Dependent Decay of mRNA and Ribosomal RNA during Platelet Aging and Its Correlation with Translation Activity

Journal: PLoS ONE

doi: 10.1371/journal.pone.0148064

Fate of human PLT RNAs in transfusion bags. Transfusion bags were incubated and sampled at 22°C or 37°C for 0, 6, 20 or 48 h. PLT activation was checked by FC after staining with FITC-conjugated annexin V or anti-P-selectin mAb. Total RNA was extracted and analyzed on a Bioanalyzer 2100 and electropherograms of the extracted RNA are shown. The 28S/18S RNA ratios are indicated between the rRNA peaks and the percentages of small RNAs are shown above the small RNA peaks. AnnV and P-Sel, percentage of annexin V- and P-selectin-positive PLTs (ND, not determined).
Figure Legend Snippet: Fate of human PLT RNAs in transfusion bags. Transfusion bags were incubated and sampled at 22°C or 37°C for 0, 6, 20 or 48 h. PLT activation was checked by FC after staining with FITC-conjugated annexin V or anti-P-selectin mAb. Total RNA was extracted and analyzed on a Bioanalyzer 2100 and electropherograms of the extracted RNA are shown. The 28S/18S RNA ratios are indicated between the rRNA peaks and the percentages of small RNAs are shown above the small RNA peaks. AnnV and P-Sel, percentage of annexin V- and P-selectin-positive PLTs (ND, not determined).

Techniques Used: Incubation, Activation Assay, Staining

5) Product Images from "Preservation of Multiple Mammalian Tissues to Maximize Science Return from Ground Based and Spaceflight Experiments"

Article Title: Preservation of Multiple Mammalian Tissues to Maximize Science Return from Ground Based and Spaceflight Experiments

Journal: PLoS ONE

doi: 10.1371/journal.pone.0167391

Effects of preservation times post-euthanasia on RNA quality in spleen and liver. Spleens and livers were collected from up to 3 minutes (Positive Control), 9 minutes (Set 1), and 25 minutes (Set 2) post-euthanasia. Set 1 and 2 time points were selected to simulate anticipated dissection timing in microgravity. Spleens were preserved in RNAlater, and livers were frozen on dry ice. All samples were stored at -80°C, and analyzed after 3.5 months for spleen and 4.5 months for liver. RNA quality was measured by calculating the Bioanalyzer-based RNA integrity number (RIN) using the Bioanalyzer 2100. Samples harvested up to 25 minutes post-euthanasia, and stored for less than 5 months at -80°C, yielded RIN values greater than 8. Data sets were assessed for normality using the Shapiro-Wilk test, followed by the Kruskal-Wallis test. Values shown are medians within interquartile (boxes) and full range (whiskers). Spleens: n = 4, 9, and 8 for Control, Set 1 and 2, respectively. Livers: n = 3, 10, and 10 for Control, Set 1 and 2, respectively.
Figure Legend Snippet: Effects of preservation times post-euthanasia on RNA quality in spleen and liver. Spleens and livers were collected from up to 3 minutes (Positive Control), 9 minutes (Set 1), and 25 minutes (Set 2) post-euthanasia. Set 1 and 2 time points were selected to simulate anticipated dissection timing in microgravity. Spleens were preserved in RNAlater, and livers were frozen on dry ice. All samples were stored at -80°C, and analyzed after 3.5 months for spleen and 4.5 months for liver. RNA quality was measured by calculating the Bioanalyzer-based RNA integrity number (RIN) using the Bioanalyzer 2100. Samples harvested up to 25 minutes post-euthanasia, and stored for less than 5 months at -80°C, yielded RIN values greater than 8. Data sets were assessed for normality using the Shapiro-Wilk test, followed by the Kruskal-Wallis test. Values shown are medians within interquartile (boxes) and full range (whiskers). Spleens: n = 4, 9, and 8 for Control, Set 1 and 2, respectively. Livers: n = 3, 10, and 10 for Control, Set 1 and 2, respectively.

Techniques Used: Preserving, Positive Control, Dissection

6) Product Images from "Gene expression dataset for whole cochlea of Macaca fascicularis"

Article Title: Gene expression dataset for whole cochlea of Macaca fascicularis

Journal: Scientific Reports

doi: 10.1038/s41598-018-33985-9

Schematic procedures to extract cochlear signature genes from  M. fascicularis . ( a ) A dissected cochlea along with the modiolus. Tissues shown within the green dotted line were dissected out as whole cochlea and subjected to RNA extraction. Scale bar = 1 cm. ( b ) Histochemical image of a  M. fascicularis  cochlea stained with hematoxylin and eosin to show that the dissected “whole cochlea” in ( a ) corresponds to the membranous tissues of the cochlea. Scale bar = 500 μm. ( c ) Evaluation of the quality of RNA extracted from the left cochlea, as assessed with a Bioanalyzer 2100. Arrowheads indicate peaks of 18S and 28S rRNA. ( d ) Procedures of the analysis. Individual gene expression data in the left and right cochleae using the (experiment 1, top) macaque or (experiment 2, bottom) human microarray were compared with averaged expression levels of three or one macaque animals in duplicate and/or pooled human tissues or cells to extract probes that had expression levels  > 2-fold compared with the average of all the tissues and  P
Figure Legend Snippet: Schematic procedures to extract cochlear signature genes from M. fascicularis . ( a ) A dissected cochlea along with the modiolus. Tissues shown within the green dotted line were dissected out as whole cochlea and subjected to RNA extraction. Scale bar = 1 cm. ( b ) Histochemical image of a M. fascicularis cochlea stained with hematoxylin and eosin to show that the dissected “whole cochlea” in ( a ) corresponds to the membranous tissues of the cochlea. Scale bar = 500 μm. ( c ) Evaluation of the quality of RNA extracted from the left cochlea, as assessed with a Bioanalyzer 2100. Arrowheads indicate peaks of 18S and 28S rRNA. ( d ) Procedures of the analysis. Individual gene expression data in the left and right cochleae using the (experiment 1, top) macaque or (experiment 2, bottom) human microarray were compared with averaged expression levels of three or one macaque animals in duplicate and/or pooled human tissues or cells to extract probes that had expression levels > 2-fold compared with the average of all the tissues and P

Techniques Used: RNA Extraction, Staining, Expressing, Microarray

7) Product Images from "Towards Improving Embryo Prioritization: Parallel Next Generation Sequencing of DNA and RNA from a Single Trophectoderm Biopsy"

Article Title: Towards Improving Embryo Prioritization: Parallel Next Generation Sequencing of DNA and RNA from a Single Trophectoderm Biopsy

Journal: Scientific Reports

doi: 10.1038/s41598-019-39111-7

Detailed workflow of the cell lysis optimization to obtain both high-quality gDNA and mRNA from the same TE biopsy. TE-A is the “clinically representative” control biopsy, lysed and processed using the standard clinical workflow for PGT-A. TE-B is the test biopsy, where cells are lysed with either SMART (Method 1) or SurePlex® (Method 2) kits. The lysate was split and processed according to the standard SurePlex® protocol for gDNA amplification, or the standard SMART-seq® protocol for cDNA synthesis. Lysis of biopsied cells with SurePlex® yields high-quality gDNA and mRNA. From each blastocyst cDNA was synthesized, amplified, and its integrity/quality was assessed by BioAnalyzer 2100 (Agilent Technologies, CA). All samples, regardless of lysis method, produced high-quality cDNA. However, only the sample lysed using SurePlex® (Method 2) produced both high-quality cDNA and gDNA which passed all clinical quality control metrics after NGS using VeriSeq® kit (highlighted with blue arrows).
Figure Legend Snippet: Detailed workflow of the cell lysis optimization to obtain both high-quality gDNA and mRNA from the same TE biopsy. TE-A is the “clinically representative” control biopsy, lysed and processed using the standard clinical workflow for PGT-A. TE-B is the test biopsy, where cells are lysed with either SMART (Method 1) or SurePlex® (Method 2) kits. The lysate was split and processed according to the standard SurePlex® protocol for gDNA amplification, or the standard SMART-seq® protocol for cDNA synthesis. Lysis of biopsied cells with SurePlex® yields high-quality gDNA and mRNA. From each blastocyst cDNA was synthesized, amplified, and its integrity/quality was assessed by BioAnalyzer 2100 (Agilent Technologies, CA). All samples, regardless of lysis method, produced high-quality cDNA. However, only the sample lysed using SurePlex® (Method 2) produced both high-quality cDNA and gDNA which passed all clinical quality control metrics after NGS using VeriSeq® kit (highlighted with blue arrows).

Techniques Used: Lysis, Amplification, Synthesized, Produced, Next-Generation Sequencing

8) Product Images from "Antibody production using a ciliate generates unusual antibody glycoforms displaying enhanced cell-killing activity"

Article Title: Antibody production using a ciliate generates unusual antibody glycoforms displaying enhanced cell-killing activity

Journal: mAbs

doi: 10.1080/19420862.2016.1228504

Expression, purification and glycoform separation of anti-CD20 antibody expressed by T. thermophila . (A) Two different expression cassettes in 2 different vectors were used to generate transgenic T. thermophila strains. The pAX_hNeoR_HC plasmid contains the full length cDNA encoding the heavy chain of anti-CD20, fused to the signal sequence of Tetrahymena PGP1 (signal peptide, amino acids 1–18), flanked by a ∼1 kb MTT1 promoter active sequence and the BTU2 terminator (∼350bp). The pKOIX_B_LC plasmid contains the full length cDNA encoding the light chain gene of anti-CD20, fused to the signal sequence of Tetrahymena PGP1 (signal peptide, amino acids 1–18), controlled by a ∼1.2 kb MTT5 promoter active sequence and the BTU2 terminator (∼350 bp). The whole expression cassette is flanked by DHFR-TS 3′ and 5′ integration sequences (each ∼1.5 kb). lx, lox promoter cassette; CmR, chloramphenicol resistance cassette, H4, Histon promotor, Ig-HC, immunoglobulin heavy chain, Ig-LC, immunoglobulin light chain, DHFR, Dihydrofolatreductase, BsdR, blasticidin resistance cassette, hNeoR, neomycin resistance cassette, BT, β tubulin terminator sequence. (B-D) Tetrahymena anti-CD20 antibody was purified from cadmium-induced cell culture supernatant using Protein A affinity chromatography. Supernatant (SN), flow-through fraction (W) and eluate fraction (E) were analyzed by SDS-PAGE and silver staining (B) and immune blot using HRP conjugated anti-human IgG antibody (C). Full IgG band of Tt /C2B8 was compared to MabThera (MT) full IgG band. Purified Tt /C2B8 and MabThera® were analyzed regarding their N-linked glycan occupancy using Bioanalyzer 2100. Antibody samples were deglycosylated by PNGaseF treatment (deglyc.) and separated under reduced (red.) and nonreduced (non red.) conditions (D). M, prestained standard. (E) Chromatogram of a representative HPLC - CEX resulting in separation of anti-CD20 antibody variants differing in heavy chain N-linked glycan occupancy. A gradient of salt at pH 4.0 was used for elution. Antibody samples from separated peaks (P1 – P3) were analyzed using Bioanalyzer 2100 (P1, 99% glycosylated HC; P2 51% glycosylated HC; P3 24% glycosylated HC, MT, MabThera®).
Figure Legend Snippet: Expression, purification and glycoform separation of anti-CD20 antibody expressed by T. thermophila . (A) Two different expression cassettes in 2 different vectors were used to generate transgenic T. thermophila strains. The pAX_hNeoR_HC plasmid contains the full length cDNA encoding the heavy chain of anti-CD20, fused to the signal sequence of Tetrahymena PGP1 (signal peptide, amino acids 1–18), flanked by a ∼1 kb MTT1 promoter active sequence and the BTU2 terminator (∼350bp). The pKOIX_B_LC plasmid contains the full length cDNA encoding the light chain gene of anti-CD20, fused to the signal sequence of Tetrahymena PGP1 (signal peptide, amino acids 1–18), controlled by a ∼1.2 kb MTT5 promoter active sequence and the BTU2 terminator (∼350 bp). The whole expression cassette is flanked by DHFR-TS 3′ and 5′ integration sequences (each ∼1.5 kb). lx, lox promoter cassette; CmR, chloramphenicol resistance cassette, H4, Histon promotor, Ig-HC, immunoglobulin heavy chain, Ig-LC, immunoglobulin light chain, DHFR, Dihydrofolatreductase, BsdR, blasticidin resistance cassette, hNeoR, neomycin resistance cassette, BT, β tubulin terminator sequence. (B-D) Tetrahymena anti-CD20 antibody was purified from cadmium-induced cell culture supernatant using Protein A affinity chromatography. Supernatant (SN), flow-through fraction (W) and eluate fraction (E) were analyzed by SDS-PAGE and silver staining (B) and immune blot using HRP conjugated anti-human IgG antibody (C). Full IgG band of Tt /C2B8 was compared to MabThera (MT) full IgG band. Purified Tt /C2B8 and MabThera® were analyzed regarding their N-linked glycan occupancy using Bioanalyzer 2100. Antibody samples were deglycosylated by PNGaseF treatment (deglyc.) and separated under reduced (red.) and nonreduced (non red.) conditions (D). M, prestained standard. (E) Chromatogram of a representative HPLC - CEX resulting in separation of anti-CD20 antibody variants differing in heavy chain N-linked glycan occupancy. A gradient of salt at pH 4.0 was used for elution. Antibody samples from separated peaks (P1 – P3) were analyzed using Bioanalyzer 2100 (P1, 99% glycosylated HC; P2 51% glycosylated HC; P3 24% glycosylated HC, MT, MabThera®).

Techniques Used: Expressing, Purification, Transgenic Assay, Plasmid Preparation, Sequencing, Cell Culture, Affinity Chromatography, Flow Cytometry, SDS Page, Silver Staining, High Performance Liquid Chromatography

9) Product Images from "RNase L Cleavage Products Promote Switch from Autophagy to Apoptosis by Caspase-Mediated Cleavage of Beclin-1"

Article Title: RNase L Cleavage Products Promote Switch from Autophagy to Apoptosis by Caspase-Mediated Cleavage of Beclin-1

Journal: International Journal of Molecular Sciences

doi: 10.3390/ijms160817611

Cleavage of Beclin-1 in RNase L-mediated cross-talk between autophagy and apoptosis. HT1080 cells were transfected with 10 µM of 2–5A or 2 µg/mL of PolyI:C and ( A ) RNase L-mediated cleavage of rRNA (arrows) was analyzed on RNA chips using the Agilent Bioanalyzer 2100 after 6 h. Cell viability was determined at indicated times by ( B ) MTT colorimetric assays, ( C ) trypan blue dye exclusion assay normalized to control cells or ( D ) uptake of PI by dying cells as measured by flow cytometry after staining with PI. Results are representative of three independent experiments performed in triplicate ± SD; ( E ) Cleavage of Caspase 3 and PARP in cell lysates from 2–5A or PolyI:C transfected cells was analyzed on immunoblots and normalized to β-actin levels; ( F ) Caspase 3/7 activity was measured in 2–5A transfected cells at indicated times using rhodamine-labeled caspase-3 and -7 substrate (ApoONE homogenous caspase-3 and -7 assay kit (Promega). Results are representative of three independent experiments performed in triplicate ± SD; ( G ) GFP-LC3 expressing HT1080 cells were mock treated, transfected with 10 µM of 2–5A or 2 µg/mL of PolyI:C for indicated times and the percentage of GFP + cells showing puncta formation compared to mock treated cells was analyzed. Results shown represent mean ± SEM for three experiments and at least 100 cells were analyzed per assay, p values are shown as compared with mock treated cells; ( H ) Cleavage of Beclin-1 was monitored in response to 2–5A or PolyI:C for indicated times on immunoblots and normalized to β-actin levels; ( I ) HT1080 cells expressing Flag-Beclin-1 were pretreated with zVAD-FMK (20 µM) or not for 1 h followed by 2 µg/mL of PolyI:C for indicated times. Cleavage of Beclin-1 was determined on immunoblots and normalized to β-actin levels. Results are representative of three independent experiments. Student’s t test was used to determine p values. * p
Figure Legend Snippet: Cleavage of Beclin-1 in RNase L-mediated cross-talk between autophagy and apoptosis. HT1080 cells were transfected with 10 µM of 2–5A or 2 µg/mL of PolyI:C and ( A ) RNase L-mediated cleavage of rRNA (arrows) was analyzed on RNA chips using the Agilent Bioanalyzer 2100 after 6 h. Cell viability was determined at indicated times by ( B ) MTT colorimetric assays, ( C ) trypan blue dye exclusion assay normalized to control cells or ( D ) uptake of PI by dying cells as measured by flow cytometry after staining with PI. Results are representative of three independent experiments performed in triplicate ± SD; ( E ) Cleavage of Caspase 3 and PARP in cell lysates from 2–5A or PolyI:C transfected cells was analyzed on immunoblots and normalized to β-actin levels; ( F ) Caspase 3/7 activity was measured in 2–5A transfected cells at indicated times using rhodamine-labeled caspase-3 and -7 substrate (ApoONE homogenous caspase-3 and -7 assay kit (Promega). Results are representative of three independent experiments performed in triplicate ± SD; ( G ) GFP-LC3 expressing HT1080 cells were mock treated, transfected with 10 µM of 2–5A or 2 µg/mL of PolyI:C for indicated times and the percentage of GFP + cells showing puncta formation compared to mock treated cells was analyzed. Results shown represent mean ± SEM for three experiments and at least 100 cells were analyzed per assay, p values are shown as compared with mock treated cells; ( H ) Cleavage of Beclin-1 was monitored in response to 2–5A or PolyI:C for indicated times on immunoblots and normalized to β-actin levels; ( I ) HT1080 cells expressing Flag-Beclin-1 were pretreated with zVAD-FMK (20 µM) or not for 1 h followed by 2 µg/mL of PolyI:C for indicated times. Cleavage of Beclin-1 was determined on immunoblots and normalized to β-actin levels. Results are representative of three independent experiments. Student’s t test was used to determine p values. * p

Techniques Used: Transfection, MTT Assay, Exclusion Assay, Flow Cytometry, Cytometry, Staining, Western Blot, Activity Assay, Labeling, Expressing

10) Product Images from "Towards Improving Embryo Prioritization: Parallel Next Generation Sequencing of DNA and RNA from a Single Trophectoderm Biopsy"

Article Title: Towards Improving Embryo Prioritization: Parallel Next Generation Sequencing of DNA and RNA from a Single Trophectoderm Biopsy

Journal: Scientific Reports

doi: 10.1038/s41598-019-39111-7

Detailed workflow of the cell lysis optimization to obtain both high-quality gDNA and mRNA from the same TE biopsy. TE-A is the “clinically representative” control biopsy, lysed and processed using the standard clinical workflow for PGT-A. TE-B is the test biopsy, where cells are lysed with either SMART (Method 1) or SurePlex® (Method 2) kits. The lysate was split and processed according to the standard SurePlex® protocol for gDNA amplification, or the standard SMART-seq® protocol for cDNA synthesis. Lysis of biopsied cells with SurePlex® yields high-quality gDNA and mRNA. From each blastocyst cDNA was synthesized, amplified, and its integrity/quality was assessed by BioAnalyzer 2100 (Agilent Technologies, CA). All samples, regardless of lysis method, produced high-quality cDNA. However, only the sample lysed using SurePlex® (Method 2) produced both high-quality cDNA and gDNA which passed all clinical quality control metrics after NGS using VeriSeq® kit (highlighted with blue arrows).
Figure Legend Snippet: Detailed workflow of the cell lysis optimization to obtain both high-quality gDNA and mRNA from the same TE biopsy. TE-A is the “clinically representative” control biopsy, lysed and processed using the standard clinical workflow for PGT-A. TE-B is the test biopsy, where cells are lysed with either SMART (Method 1) or SurePlex® (Method 2) kits. The lysate was split and processed according to the standard SurePlex® protocol for gDNA amplification, or the standard SMART-seq® protocol for cDNA synthesis. Lysis of biopsied cells with SurePlex® yields high-quality gDNA and mRNA. From each blastocyst cDNA was synthesized, amplified, and its integrity/quality was assessed by BioAnalyzer 2100 (Agilent Technologies, CA). All samples, regardless of lysis method, produced high-quality cDNA. However, only the sample lysed using SurePlex® (Method 2) produced both high-quality cDNA and gDNA which passed all clinical quality control metrics after NGS using VeriSeq® kit (highlighted with blue arrows).

Techniques Used: Lysis, Amplification, Synthesized, Produced, Next-Generation Sequencing

11) Product Images from "Towards Improving Embryo Prioritization: Parallel Next Generation Sequencing of DNA and RNA from a Single Trophectoderm Biopsy"

Article Title: Towards Improving Embryo Prioritization: Parallel Next Generation Sequencing of DNA and RNA from a Single Trophectoderm Biopsy

Journal: Scientific Reports

doi: 10.1038/s41598-019-39111-7

Detailed workflow of the cell lysis optimization to obtain both high-quality gDNA and mRNA from the same TE biopsy. TE-A is the “clinically representative” control biopsy, lysed and processed using the standard clinical workflow for PGT-A. TE-B is the test biopsy, where cells are lysed with either SMART (Method 1) or SurePlex® (Method 2) kits. The lysate was split and processed according to the standard SurePlex® protocol for gDNA amplification, or the standard SMART-seq® protocol for cDNA synthesis. Lysis of biopsied cells with SurePlex® yields high-quality gDNA and mRNA. From each blastocyst cDNA was synthesized, amplified, and its integrity/quality was assessed by BioAnalyzer 2100 (Agilent Technologies, CA). All samples, regardless of lysis method, produced high-quality cDNA. However, only the sample lysed using SurePlex® (Method 2) produced both high-quality cDNA and gDNA which passed all clinical quality control metrics after NGS using VeriSeq® kit (highlighted with blue arrows).
Figure Legend Snippet: Detailed workflow of the cell lysis optimization to obtain both high-quality gDNA and mRNA from the same TE biopsy. TE-A is the “clinically representative” control biopsy, lysed and processed using the standard clinical workflow for PGT-A. TE-B is the test biopsy, where cells are lysed with either SMART (Method 1) or SurePlex® (Method 2) kits. The lysate was split and processed according to the standard SurePlex® protocol for gDNA amplification, or the standard SMART-seq® protocol for cDNA synthesis. Lysis of biopsied cells with SurePlex® yields high-quality gDNA and mRNA. From each blastocyst cDNA was synthesized, amplified, and its integrity/quality was assessed by BioAnalyzer 2100 (Agilent Technologies, CA). All samples, regardless of lysis method, produced high-quality cDNA. However, only the sample lysed using SurePlex® (Method 2) produced both high-quality cDNA and gDNA which passed all clinical quality control metrics after NGS using VeriSeq® kit (highlighted with blue arrows).

Techniques Used: Lysis, Amplification, Synthesized, Produced, Next-Generation Sequencing

12) Product Images from "Proliferation Cycle Causes Age Dependent Mitochondrial Deficiencies and Contributes to the Aging of Stem Cells"

Article Title: Proliferation Cycle Causes Age Dependent Mitochondrial Deficiencies and Contributes to the Aging of Stem Cells

Journal: Genes

doi: 10.3390/genes8120397

Mitochondrial DNA (mtDNA) rearrangements and impaired mtDNA replication in ovaries of aged flies. ( A ) Schematic drawing of  Drosophila melanogaster  (Dm.) mtDNA. Enzyme sites of HindIII (H) and XhoI (X) are indicated. Sizes of digested fragments are also labelled. ( B ) Representative gel image of rolling cycle amplification of mtDNA (arrowhead) and DNA marker (M, kb). ( C ) Pattern of rolling cycle amplification (RCA) amplified mtDNA digested by XhoI and HindIII. The 5.8 kb fragment spanning the AT-rich region was recovered for restriction fragment length polymorphism (RFLP) analysis. ( D ) Schematic map of SspI site on 5.8 kb AT-rich region. ( E ) densitometry plot of SspI digestion pattern of 5.8 fragments spanning the AT-rich region from young (2-day-old) and old (60-day-old) ovaries, analyzed by Agilent Bioanalyzer 2100. Note the difference of bands (open arrowheads) demonstrating the rearrangements of AT-rich regions in aged ovaries. DNA dye standard (closed arrowheads) and DNA ladder are marked (M, bp). ( F ) Representative images showing 5-ethynyl-2’-deoxyuridine (EdU) incorporation into mtDNA (green puncta, arrowheads) in ovaries (dashed line, anterior toward left) from young (2-day-old) and old (40-day-old) female flies. Note that the number of EdU puncta was dramatically reduced in germarium from old fly. Arrowhead: mtDNA; arrow: nuclear DNA; scale bar: 10 µm.
Figure Legend Snippet: Mitochondrial DNA (mtDNA) rearrangements and impaired mtDNA replication in ovaries of aged flies. ( A ) Schematic drawing of Drosophila melanogaster (Dm.) mtDNA. Enzyme sites of HindIII (H) and XhoI (X) are indicated. Sizes of digested fragments are also labelled. ( B ) Representative gel image of rolling cycle amplification of mtDNA (arrowhead) and DNA marker (M, kb). ( C ) Pattern of rolling cycle amplification (RCA) amplified mtDNA digested by XhoI and HindIII. The 5.8 kb fragment spanning the AT-rich region was recovered for restriction fragment length polymorphism (RFLP) analysis. ( D ) Schematic map of SspI site on 5.8 kb AT-rich region. ( E ) densitometry plot of SspI digestion pattern of 5.8 fragments spanning the AT-rich region from young (2-day-old) and old (60-day-old) ovaries, analyzed by Agilent Bioanalyzer 2100. Note the difference of bands (open arrowheads) demonstrating the rearrangements of AT-rich regions in aged ovaries. DNA dye standard (closed arrowheads) and DNA ladder are marked (M, bp). ( F ) Representative images showing 5-ethynyl-2’-deoxyuridine (EdU) incorporation into mtDNA (green puncta, arrowheads) in ovaries (dashed line, anterior toward left) from young (2-day-old) and old (40-day-old) female flies. Note that the number of EdU puncta was dramatically reduced in germarium from old fly. Arrowhead: mtDNA; arrow: nuclear DNA; scale bar: 10 µm.

Techniques Used: Amplification, Marker

13) Product Images from "Small RNA Sequencing for Profiling MicroRNAs in Long-Term Preserved Formalin-Fixed and Paraffin-Embedded Non-Small Cell Lung Cancer Tumor Specimens"

Article Title: Small RNA Sequencing for Profiling MicroRNAs in Long-Term Preserved Formalin-Fixed and Paraffin-Embedded Non-Small Cell Lung Cancer Tumor Specimens

Journal: PLoS ONE

doi: 10.1371/journal.pone.0121521

Assessment of total RNA extracted from FFPE tissues. A. Correlation between measurements of RNA yield (μg) obtained using absorbance at 260 nm or fluorescence with RiboGreen dye (n = 8). The lines of identity and linear regression (least squares method) and values and 95% confidence intervals of the Pearson coefficient ( r ) and slope of linear regression ( m );  P -values are also noted. B. Electrophoretogram of the 8 RNA samples ( A - H ). Samples were run on an Agilent Eukaryote Total RNA Pico chip on Bioanalyzer 2100. Sizes of molecular weight markers and the RNA integrity numbers are noted.
Figure Legend Snippet: Assessment of total RNA extracted from FFPE tissues. A. Correlation between measurements of RNA yield (μg) obtained using absorbance at 260 nm or fluorescence with RiboGreen dye (n = 8). The lines of identity and linear regression (least squares method) and values and 95% confidence intervals of the Pearson coefficient ( r ) and slope of linear regression ( m ); P -values are also noted. B. Electrophoretogram of the 8 RNA samples ( A - H ). Samples were run on an Agilent Eukaryote Total RNA Pico chip on Bioanalyzer 2100. Sizes of molecular weight markers and the RNA integrity numbers are noted.

Techniques Used: Formalin-fixed Paraffin-Embedded, Fluorescence, Chromatin Immunoprecipitation, Molecular Weight

14) Product Images from "Next-Generation Sequencing-Based RiboMethSeq Protocol for Analysis of tRNA 2′-O-Methylation"

Article Title: Next-Generation Sequencing-Based RiboMethSeq Protocol for Analysis of tRNA 2′-O-Methylation

Journal: Biomolecules

doi: 10.3390/biom7010013

Fragmentation profiles for E. coli and S. cerevisiae total tRNA fractions. Alkaline hydrolysis was performed for 6–12 min (as indicated on the traces). Fragments size was analyzed by capillary electrophoresis on a Bioanalyzer 2100 (Agilent, Santa Clara, CA, USA) Small RNA Chip. FU: fluorescence unit.
Figure Legend Snippet: Fragmentation profiles for E. coli and S. cerevisiae total tRNA fractions. Alkaline hydrolysis was performed for 6–12 min (as indicated on the traces). Fragments size was analyzed by capillary electrophoresis on a Bioanalyzer 2100 (Agilent, Santa Clara, CA, USA) Small RNA Chip. FU: fluorescence unit.

Techniques Used: Electrophoresis, Chromatin Immunoprecipitation, Fluorescence

15) Product Images from "Towards Improving Embryo Prioritization: Parallel Next Generation Sequencing of DNA and RNA from a Single Trophectoderm Biopsy"

Article Title: Towards Improving Embryo Prioritization: Parallel Next Generation Sequencing of DNA and RNA from a Single Trophectoderm Biopsy

Journal: Scientific Reports

doi: 10.1038/s41598-019-39111-7

Detailed workflow of the cell lysis optimization to obtain both high-quality gDNA and mRNA from the same TE biopsy. TE-A is the “clinically representative” control biopsy, lysed and processed using the standard clinical workflow for PGT-A. TE-B is the test biopsy, where cells are lysed with either SMART (Method 1) or SurePlex® (Method 2) kits. The lysate was split and processed according to the standard SurePlex® protocol for gDNA amplification, or the standard SMART-seq® protocol for cDNA synthesis. Lysis of biopsied cells with SurePlex® yields high-quality gDNA and mRNA. From each blastocyst cDNA was synthesized, amplified, and its integrity/quality was assessed by BioAnalyzer 2100 (Agilent Technologies, CA). All samples, regardless of lysis method, produced high-quality cDNA. However, only the sample lysed using SurePlex® (Method 2) produced both high-quality cDNA and gDNA which passed all clinical quality control metrics after NGS using VeriSeq® kit (highlighted with blue arrows).
Figure Legend Snippet: Detailed workflow of the cell lysis optimization to obtain both high-quality gDNA and mRNA from the same TE biopsy. TE-A is the “clinically representative” control biopsy, lysed and processed using the standard clinical workflow for PGT-A. TE-B is the test biopsy, where cells are lysed with either SMART (Method 1) or SurePlex® (Method 2) kits. The lysate was split and processed according to the standard SurePlex® protocol for gDNA amplification, or the standard SMART-seq® protocol for cDNA synthesis. Lysis of biopsied cells with SurePlex® yields high-quality gDNA and mRNA. From each blastocyst cDNA was synthesized, amplified, and its integrity/quality was assessed by BioAnalyzer 2100 (Agilent Technologies, CA). All samples, regardless of lysis method, produced high-quality cDNA. However, only the sample lysed using SurePlex® (Method 2) produced both high-quality cDNA and gDNA which passed all clinical quality control metrics after NGS using VeriSeq® kit (highlighted with blue arrows).

Techniques Used: Lysis, Amplification, Synthesized, Produced, Next-Generation Sequencing

16) Product Images from "A comparison of commercially-available automated and manual extraction kits for the isolation of total RNA from small tissue samples"

Article Title: A comparison of commercially-available automated and manual extraction kits for the isolation of total RNA from small tissue samples

Journal: BMC Biotechnology

doi: 10.1186/s12896-014-0094-8

A representative example of the gel images produced during RNA quality checks performed via the Bioanalyzer 2100.  The composite gel image was generated from the results of RNA quality assessments performed on RNA extracted from larvae samples. For each kit, the lane corresponding to the sample that best represented the “average” RNA yielded is included. The first number reported for each kit represents the RIN value of the sample shown, while the numbers below represent the mean RIN value (± standard deviation) for all larvae samples extracted via each kit. Note that the lanes shown in the image were not obtained from samples run on the same Nanochip resulting in the misalignment of the 18S and 28S bands between some of the lanes.
Figure Legend Snippet: A representative example of the gel images produced during RNA quality checks performed via the Bioanalyzer 2100. The composite gel image was generated from the results of RNA quality assessments performed on RNA extracted from larvae samples. For each kit, the lane corresponding to the sample that best represented the “average” RNA yielded is included. The first number reported for each kit represents the RIN value of the sample shown, while the numbers below represent the mean RIN value (± standard deviation) for all larvae samples extracted via each kit. Note that the lanes shown in the image were not obtained from samples run on the same Nanochip resulting in the misalignment of the 18S and 28S bands between some of the lanes.

Techniques Used: Produced, Generated, Standard Deviation

17) Product Images from "How do megakaryocytic microparticles target and deliver cargo to alter the fate of hematopoietic stem cells?"

Article Title: How do megakaryocytic microparticles target and deliver cargo to alter the fate of hematopoietic stem cells?

Journal: Journal of controlled release : official journal of the Controlled Release Society

doi: 10.1016/j.jconrel.2016.12.021

RNA profile of Mk and MkMP with or without RNase treatment (A) MkMPs were first treated with or without RNase I for 2 hours at 37 °C. After incubated with RNase inhibitors and washed with IMDM thrice, total RNA were isolated from MkMPs and RNA profiles were analyzed by Bioanalyzer 2100. (B) RNA profile of d12 Mks. (A) presents the data from two biological replicates to demonstrate the exquisite reproducibility of the RNA profiles before and after RNase treatment. The symbol (#) in (A) and (B) marks the range of small RNAs. 18S and 28S represent rRNA, while (δ) in (A) and (B) marks the ladder of 25 nt. (C) RNA profile of ladders for size verification. (D) Total RNA concentration from MkMPs treated with or without were measured by Qubit RNA HS kit. The measurement shows 65.5% of RNA being digested after RNA treatment. The data represent two biological replicates ± standard deviation.
Figure Legend Snippet: RNA profile of Mk and MkMP with or without RNase treatment (A) MkMPs were first treated with or without RNase I for 2 hours at 37 °C. After incubated with RNase inhibitors and washed with IMDM thrice, total RNA were isolated from MkMPs and RNA profiles were analyzed by Bioanalyzer 2100. (B) RNA profile of d12 Mks. (A) presents the data from two biological replicates to demonstrate the exquisite reproducibility of the RNA profiles before and after RNase treatment. The symbol (#) in (A) and (B) marks the range of small RNAs. 18S and 28S represent rRNA, while (δ) in (A) and (B) marks the ladder of 25 nt. (C) RNA profile of ladders for size verification. (D) Total RNA concentration from MkMPs treated with or without were measured by Qubit RNA HS kit. The measurement shows 65.5% of RNA being digested after RNA treatment. The data represent two biological replicates ± standard deviation.

Techniques Used: Incubation, Isolation, Concentration Assay, Standard Deviation

18) Product Images from "RNase L Induces Autophagy via c-Jun N-terminal Kinase and Double-stranded RNA-dependent Protein Kinase Signaling Pathways *"

Article Title: RNase L Induces Autophagy via c-Jun N-terminal Kinase and Double-stranded RNA-dependent Protein Kinase Signaling Pathways *

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M112.399964

2–5A-mediated activation of RNase L induces autophagy. A , HT1080 cells were transfected with 10 μ m  of 2–5A and cleavage of rRNA (shown by  arrows ) was analyzed on RNA chips using Agilent Bioanalyzer 2100.  B , 2–5A was transfected
Figure Legend Snippet: 2–5A-mediated activation of RNase L induces autophagy. A , HT1080 cells were transfected with 10 μ m of 2–5A and cleavage of rRNA (shown by arrows ) was analyzed on RNA chips using Agilent Bioanalyzer 2100. B , 2–5A was transfected

Techniques Used: Activation Assay, Transfection

19) Product Images from "Gene Expression Analysis of In Vivo Fluorescent Cells"

Article Title: Gene Expression Analysis of In Vivo Fluorescent Cells

Journal: PLoS ONE

doi: 10.1371/journal.pone.0001151

RNA analysis by the Bioanalyzer 2100. ( A ) RNA isolated from fixed tissue by the optimized proteinase K/acid phenol method described here. ( B ) RNA isolated from frozen tissue by the optimized proteinase K/acid phenol method. ( C ) RNA isolated from fixed tissue by TRIzol method. ( D ) RNA isolated from fixed tissue by RNeasy Micro Kit.  (E)  One round of amplification of RNA isolated from fixed tissue by the optimized proteinase K/acid phenol method. ( F ) One round of amplification of Ambion Control RNA. ( G ) Two rounds of amplification of RNA isolated from fixed tissue by the optimized proteinase K/acid phenol method. ( H ) RNA ladder: first peak is RNA marker, next mark 200, 500, 1000, 2000, 4000 and 6000 nt.
Figure Legend Snippet: RNA analysis by the Bioanalyzer 2100. ( A ) RNA isolated from fixed tissue by the optimized proteinase K/acid phenol method described here. ( B ) RNA isolated from frozen tissue by the optimized proteinase K/acid phenol method. ( C ) RNA isolated from fixed tissue by TRIzol method. ( D ) RNA isolated from fixed tissue by RNeasy Micro Kit. (E) One round of amplification of RNA isolated from fixed tissue by the optimized proteinase K/acid phenol method. ( F ) One round of amplification of Ambion Control RNA. ( G ) Two rounds of amplification of RNA isolated from fixed tissue by the optimized proteinase K/acid phenol method. ( H ) RNA ladder: first peak is RNA marker, next mark 200, 500, 1000, 2000, 4000 and 6000 nt.

Techniques Used: Isolation, Amplification, Marker

20) Product Images from "Exploiting Pre-rRNA Processing in Diamond Blackfan Anemia Gene Discovery and Diagnosis"

Article Title: Exploiting Pre-rRNA Processing in Diamond Blackfan Anemia Gene Discovery and Diagnosis

Journal: American journal of hematology

doi: 10.1002/ajh.23807

Representative Agilent Bioanalyzer 2100 electrophoretogram data demonstrates prominent peaks corresponding to 18S and 28S rRNA, with a minor peak just larger than 28S rRNA corresponding to 32S pre-RNA, that was evident in analysis of total RNA from ConA-stimulated
Figure Legend Snippet: Representative Agilent Bioanalyzer 2100 electrophoretogram data demonstrates prominent peaks corresponding to 18S and 28S rRNA, with a minor peak just larger than 28S rRNA corresponding to 32S pre-RNA, that was evident in analysis of total RNA from ConA-stimulated

Techniques Used:

21) Product Images from "Gene Expression Analysis of In Vivo Fluorescent Cells"

Article Title: Gene Expression Analysis of In Vivo Fluorescent Cells

Journal: PLoS ONE

doi: 10.1371/journal.pone.0001151

RNA analysis by the Bioanalyzer 2100. ( A ) RNA isolated from fixed tissue by the optimized proteinase K/acid phenol method described here. ( B ) RNA isolated from frozen tissue by the optimized proteinase K/acid phenol method. ( C ) RNA isolated from fixed tissue by TRIzol method. ( D ) RNA isolated from fixed tissue by RNeasy Micro Kit.  (E)  One round of amplification of RNA isolated from fixed tissue by the optimized proteinase K/acid phenol method. ( F ) One round of amplification of Ambion Control RNA. ( G ) Two rounds of amplification of RNA isolated from fixed tissue by the optimized proteinase K/acid phenol method. ( H ) RNA ladder: first peak is RNA marker, next mark 200, 500, 1000, 2000, 4000 and 6000 nt.
Figure Legend Snippet: RNA analysis by the Bioanalyzer 2100. ( A ) RNA isolated from fixed tissue by the optimized proteinase K/acid phenol method described here. ( B ) RNA isolated from frozen tissue by the optimized proteinase K/acid phenol method. ( C ) RNA isolated from fixed tissue by TRIzol method. ( D ) RNA isolated from fixed tissue by RNeasy Micro Kit. (E) One round of amplification of RNA isolated from fixed tissue by the optimized proteinase K/acid phenol method. ( F ) One round of amplification of Ambion Control RNA. ( G ) Two rounds of amplification of RNA isolated from fixed tissue by the optimized proteinase K/acid phenol method. ( H ) RNA ladder: first peak is RNA marker, next mark 200, 500, 1000, 2000, 4000 and 6000 nt.

Techniques Used: Isolation, Amplification, Marker

Related Articles

Centrifugation:

Article Title: Hemoprotein Bach1 regulates enhancer availability of heme oxygenase-1 gene
Article Snippet: Chromatin fixation and purification procedures were as described previously , without carrying out CsCl equilibrium centrifugation. .. Amounts of products were determined as above using the BioAnalyzer 2100 and DNA 500 chip (Agilent).

Article Title: Next-Generation Sequencing-Based RiboMethSeq Protocol for Analysis of tRNA 2′-O-Methylation
Article Snippet: After centrifugation, the pellet was washed with 80% ethanol and resuspended in nuclease-free water. .. The sizes of generated RNA fragments were assessed by capillary electrophoresis using a Small RNA chip on the Bioanalyzer 2100 (Agilent).

Amplification:

Article Title: Hemoprotein Bach1 regulates enhancer availability of heme oxygenase-1 gene
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Article Title: Towards Improving Embryo Prioritization: Parallel Next Generation Sequencing of DNA and RNA from a Single Trophectoderm Biopsy
Article Snippet: Biopsied cells lysed using the SMART-seq® protocol (I) (Takara BioInc, CA) yielded cDNA of high-quality and quantity, measured by BioAnalyzer 2100 (Agilent Technologies, CA) and fluorometer, respectively. .. Based on these findings, we established our PGT-AT method: (1) Single TE biopsy, (2) SurePlex® cell lysis mix, (3) Lysate splitting (4) Simultaneous, independent gDNA amplification and cDNA synthesis (indicated in Fig. with blue arrows).

Article Title: Proliferation Cycle Causes Age Dependent Mitochondrial Deficiencies and Contributes to the Aging of Stem Cells
Article Snippet: Paragraph title: 2.6. Rolling Cycle Amplification and Restriction Fragment Length Polymorphism analysis of mtDNA ... In addition, 5.8 kb fragment was recovered, further digested with SspI overnight and analyzed on BioAnalyzer 2100 (Agilent, Savage, MD, USA) using a High Sensitivity DNA Kit (Agilent) following the manufacturer’s protocol.

Article Title: Towards Improving Embryo Prioritization: Parallel Next Generation Sequencing of DNA and RNA from a Single Trophectoderm Biopsy
Article Snippet: .. The resulting amplified gDNA was sequenced using Nextera XT® library preparation for NGS following the VeriSeq® protocol, described in detail below (Illumina, CA). cDNA quality was assessed using BioAnalyzer 2100 (Agilent Technologies, CA). .. Table presents the results for chromosomal aberrations and sequencing quality metrics from VeriSeq® sequencing of gDNA obtained through SurePlex® kit using lysis with I. SMART-seq® (Takara BioInc, CA) or II.

Whole Genome Amplification:

Article Title: Towards Improving Embryo Prioritization: Parallel Next Generation Sequencing of DNA and RNA from a Single Trophectoderm Biopsy
Article Snippet: .. In each of these cases the TE biopsy was lysed and then split: half the lysate underwent WGA and VeriSeq® sequencing and the other half of the lysate underwent cDNA synthesis and mRNA sequencing (Table ). cDNA quality and quantity were confirmed using a BioAnalyzer 2100 (Agilent Technologies, CA). .. The WGA product quantity and quality were confirmed by fluorimeter (Qubit - ThermoFisher) and electrophoresis (2% w/v agarose, 100 V for 30 min), respectively.

Article Title: Towards Improving Embryo Prioritization: Parallel Next Generation Sequencing of DNA and RNA from a Single Trophectoderm Biopsy
Article Snippet: For lysis option II, biopsied cells were deposited into 2.5 ul of 1xPBS buffer and lysed using cell extraction enzyme mix from SurePlex® whole genome amplification (WGA) kit. .. The resulting amplified gDNA was sequenced using Nextera XT® library preparation for NGS following the VeriSeq® protocol, described in detail below (Illumina, CA). cDNA quality was assessed using BioAnalyzer 2100 (Agilent Technologies, CA).

Polymerase Chain Reaction:

Article Title: Hemoprotein Bach1 regulates enhancer availability of heme oxygenase-1 gene
Article Snippet: .. PCR products were analyzed using the BioAnalyzer 2100 and DNA 500 chip (Agilent). .. Serial 3-fold dilutions of cDNA were used to ensure linearity of amplification.

Electrophoresis:

Article Title: Small RNA Sequencing for Profiling MicroRNAs in Long-Term Preserved Formalin-Fixed and Paraffin-Embedded Non-Small Cell Lung Cancer Tumor Specimens
Article Snippet: .. Integrity of RNA was assessed using electrophoresis on Eukaryote Total RNA Pico chip on Bioanalyzer 2100 (Agilent, Santa Clara, USA). ..

Article Title: Towards Improving Embryo Prioritization: Parallel Next Generation Sequencing of DNA and RNA from a Single Trophectoderm Biopsy
Article Snippet: In each of these cases the TE biopsy was lysed and then split: half the lysate underwent WGA and VeriSeq® sequencing and the other half of the lysate underwent cDNA synthesis and mRNA sequencing (Table ). cDNA quality and quantity were confirmed using a BioAnalyzer 2100 (Agilent Technologies, CA). .. The WGA product quantity and quality were confirmed by fluorimeter (Qubit - ThermoFisher) and electrophoresis (2% w/v agarose, 100 V for 30 min), respectively.

Article Title: Next-Generation Sequencing-Based RiboMethSeq Protocol for Analysis of tRNA 2′-O-Methylation
Article Snippet: .. The sizes of generated RNA fragments were assessed by capillary electrophoresis using a Small RNA chip on the Bioanalyzer 2100 (Agilent). .. Library Preparation RNA fragments without any gel-purification step were directly 3′-end dephosphorylated using 5 U of Antarctic phosphatase (New England Biolabs, Ipswich, MA, USA) for 30 min at 37 °C.

Quantitation Assay:

Article Title: Genome-wide profiling of the 3' ends of polyadenylated RNAs
Article Snippet: 69 Check the quality of 3'-seq library preparation by running 1 µL of sample on a Bioanalyzer 2100 with an Agilent High Sensitivity DNA Kit. .. Quantify the library either by using the Bioanalyzer estimate or other methods, such as Qubit fluorometric quantitation.

Formalin-fixed Paraffin-Embedded:

Article Title: Small RNA Sequencing for Profiling MicroRNAs in Long-Term Preserved Formalin-Fixed and Paraffin-Embedded Non-Small Cell Lung Cancer Tumor Specimens
Article Snippet: RNA extraction and quantification Total RNA was isolated from 4 sections, or approximately 3 mm3 of each tumor, using the affinity spin column-based High Pure FFPE RNA Isolation Kit (Roche, Indianapolis, USA). .. Integrity of RNA was assessed using electrophoresis on Eukaryote Total RNA Pico chip on Bioanalyzer 2100 (Agilent, Santa Clara, USA).

Activity Assay:

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Article Snippet: Paragraph title: 4.4. Monitoring RNase L Activity in Cells and Isolation and Purification of RNase L Cleaved RNAs ... RNA (2 µg) was separated on RNA chips and analyzed with Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA) as described previously [ ].

Expressing:

Article Title: Towards Improving Embryo Prioritization: Parallel Next Generation Sequencing of DNA and RNA from a Single Trophectoderm Biopsy
Article Snippet: .. The TE transcriptome obtained with PGT-AT was of high quality All tested samples produced sufficient quantity and high-quality cDNA for RNAseq, as per BioAnalyzer 2100 (Agilent Technologies, CA) (see Supplementary Fig. ), enabling us to conduct gene expression profiling. ..

Transfection:

Article Title: RNase L Cleavage Products Promote Switch from Autophagy to Apoptosis by Caspase-Mediated Cleavage of Beclin-1
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Generated:

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Reverse Transcription Polymerase Chain Reaction:

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Sonication:

Article Title: Hemoprotein Bach1 regulates enhancer availability of heme oxygenase-1 gene
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Fluorescence:

Article Title: Small RNA Sequencing for Profiling MicroRNAs in Long-Term Preserved Formalin-Fixed and Paraffin-Embedded Non-Small Cell Lung Cancer Tumor Specimens
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Isolation:

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Labeling:

Article Title: Time-Dependent Decay of mRNA and Ribosomal RNA during Platelet Aging and Its Correlation with Translation Activity
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Purification:

Article Title: RNase L Cleavage Products Promote Switch from Autophagy to Apoptosis by Caspase-Mediated Cleavage of Beclin-1
Article Snippet: Paragraph title: 4.4. Monitoring RNase L Activity in Cells and Isolation and Purification of RNase L Cleaved RNAs ... RNA (2 µg) was separated on RNA chips and analyzed with Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA) as described previously [ ].

Article Title: Hemoprotein Bach1 regulates enhancer availability of heme oxygenase-1 gene
Article Snippet: Chromatin fixation and purification procedures were as described previously , without carrying out CsCl equilibrium centrifugation. .. Amounts of products were determined as above using the BioAnalyzer 2100 and DNA 500 chip (Agilent).

Article Title: Gene expression dataset for whole cochlea of Macaca fascicularis
Article Snippet: RNA from whole membranous cochlear tissues was extracted using ISOGEN-II (Nippon Gene, Toyama, Japan) and purified using an RNeasy micro kit (QIAGEN, Hamburg, Germany). .. Quality of the RNA extracted from the cochleae was analyzed with a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA) (Fig. ).

Article Title: Time-Dependent Decay of mRNA and Ribosomal RNA during Platelet Aging and Its Correlation with Translation Activity
Article Snippet: .. RNAs from in vitro -differentiated MKs or from leukocyte- and erythrocyte-depleted PLTs were Trizol extracted and further purified on silica matrix and then quality-checked using a Bioanalyzer 2100 and an RNA 6000 Nano kit (Agilent). .. On day 8 of the protocol, as described , PLTs from saline- and DT-treated mice were analyzed. (A) Representative profiles of RNA from in vitro differentiated MKs and PLTs from DT- or saline-treated animals. (B) Distribution of RIN and 28S/18S RNA values from retPLTs. (C) Percentage of retPLTs vs 28S/18S RNA ratio plot. (D) Percentage of retPLT vs platelet RNA content (fg/platelet).

Sequencing:

Article Title: Towards Improving Embryo Prioritization: Parallel Next Generation Sequencing of DNA and RNA from a Single Trophectoderm Biopsy
Article Snippet: .. In each of these cases the TE biopsy was lysed and then split: half the lysate underwent WGA and VeriSeq® sequencing and the other half of the lysate underwent cDNA synthesis and mRNA sequencing (Table ). cDNA quality and quantity were confirmed using a BioAnalyzer 2100 (Agilent Technologies, CA). .. The WGA product quantity and quality were confirmed by fluorimeter (Qubit - ThermoFisher) and electrophoresis (2% w/v agarose, 100 V for 30 min), respectively.

Article Title: Towards Improving Embryo Prioritization: Parallel Next Generation Sequencing of DNA and RNA from a Single Trophectoderm Biopsy
Article Snippet: The resulting amplified gDNA was sequenced using Nextera XT® library preparation for NGS following the VeriSeq® protocol, described in detail below (Illumina, CA). cDNA quality was assessed using BioAnalyzer 2100 (Agilent Technologies, CA). .. Table presents the results for chromosomal aberrations and sequencing quality metrics from VeriSeq® sequencing of gDNA obtained through SurePlex® kit using lysis with I. SMART-seq® (Takara BioInc, CA) or II.

Lysis:

Article Title: Towards Improving Embryo Prioritization: Parallel Next Generation Sequencing of DNA and RNA from a Single Trophectoderm Biopsy
Article Snippet: Paragraph title: Optimized TE biopsy cell lysis ... Biopsied cells lysed using the SMART-seq® protocol (I) (Takara BioInc, CA) yielded cDNA of high-quality and quantity, measured by BioAnalyzer 2100 (Agilent Technologies, CA) and fluorometer, respectively.

Mouse Assay:

Article Title: Time-Dependent Decay of mRNA and Ribosomal RNA during Platelet Aging and Its Correlation with Translation Activity
Article Snippet: RNAs from in vitro -differentiated MKs or from leukocyte- and erythrocyte-depleted PLTs were Trizol extracted and further purified on silica matrix and then quality-checked using a Bioanalyzer 2100 and an RNA 6000 Nano kit (Agilent). .. On day 8 of the protocol, as described , PLTs from saline- and DT-treated mice were analyzed. (A) Representative profiles of RNA from in vitro differentiated MKs and PLTs from DT- or saline-treated animals. (B) Distribution of RIN and 28S/18S RNA values from retPLTs. (C) Percentage of retPLTs vs 28S/18S RNA ratio plot. (D) Percentage of retPLT vs platelet RNA content (fg/platelet).

Chromatin Immunoprecipitation:

Article Title: Hemoprotein Bach1 regulates enhancer availability of heme oxygenase-1 gene
Article Snippet: .. PCR products were analyzed using the BioAnalyzer 2100 and DNA 500 chip (Agilent). .. Serial 3-fold dilutions of cDNA were used to ensure linearity of amplification.

Article Title: Hemoprotein Bach1 regulates enhancer availability of heme oxygenase-1 gene
Article Snippet: .. Amounts of products were determined as above using the BioAnalyzer 2100 and DNA 500 chip (Agilent). ..

Article Title: Small RNA Sequencing for Profiling MicroRNAs in Long-Term Preserved Formalin-Fixed and Paraffin-Embedded Non-Small Cell Lung Cancer Tumor Specimens
Article Snippet: .. Integrity of RNA was assessed using electrophoresis on Eukaryote Total RNA Pico chip on Bioanalyzer 2100 (Agilent, Santa Clara, USA). ..

Article Title: Antibody production using a ciliate generates unusual antibody glycoforms displaying enhanced cell-killing activity
Article Snippet: .. CE-SDS gel using Bioanalyzer 2100 CE-SDS of the protein probes was performed in Bioanalyzer 2100 (Agilent Technologies) on a High Sensitivity Protein Chip to determine the ratio of glycosylated and aglycosylated HC molecules in antibody samples. .. Sample preparation was performed using Agilent High Sensitivity Protein 250 Kit (Agilent Technologies) according to the manufacturer's instructions.

Article Title: Next-Generation Sequencing-Based RiboMethSeq Protocol for Analysis of tRNA 2′-O-Methylation
Article Snippet: .. The sizes of generated RNA fragments were assessed by capillary electrophoresis using a Small RNA chip on the Bioanalyzer 2100 (Agilent). .. Library Preparation RNA fragments without any gel-purification step were directly 3′-end dephosphorylated using 5 U of Antarctic phosphatase (New England Biolabs, Ipswich, MA, USA) for 30 min at 37 °C.

Software:

Article Title: Antibody production using a ciliate generates unusual antibody glycoforms displaying enhanced cell-killing activity
Article Snippet: CE-SDS gel using Bioanalyzer 2100 CE-SDS of the protein probes was performed in Bioanalyzer 2100 (Agilent Technologies) on a High Sensitivity Protein Chip to determine the ratio of glycosylated and aglycosylated HC molecules in antibody samples. .. Quantification and size analysis were based on a protein standard as a reference in every measurement using Bioanalyzer 2100 system and software.

RNA Extraction:

Article Title: Small RNA Sequencing for Profiling MicroRNAs in Long-Term Preserved Formalin-Fixed and Paraffin-Embedded Non-Small Cell Lung Cancer Tumor Specimens
Article Snippet: Paragraph title: RNA extraction and quantification ... Integrity of RNA was assessed using electrophoresis on Eukaryote Total RNA Pico chip on Bioanalyzer 2100 (Agilent, Santa Clara, USA).

Article Title: Gene expression dataset for whole cochlea of Macaca fascicularis
Article Snippet: Paragraph title: Tissue collection and RNA extraction ... Quality of the RNA extracted from the cochleae was analyzed with a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA) (Fig. ).

Sample Prep:

Article Title: Antibody production using a ciliate generates unusual antibody glycoforms displaying enhanced cell-killing activity
Article Snippet: CE-SDS gel using Bioanalyzer 2100 CE-SDS of the protein probes was performed in Bioanalyzer 2100 (Agilent Technologies) on a High Sensitivity Protein Chip to determine the ratio of glycosylated and aglycosylated HC molecules in antibody samples. .. Sample preparation was performed using Agilent High Sensitivity Protein 250 Kit (Agilent Technologies) according to the manufacturer's instructions.

In Vitro:

Article Title: Time-Dependent Decay of mRNA and Ribosomal RNA during Platelet Aging and Its Correlation with Translation Activity
Article Snippet: .. RNAs from in vitro -differentiated MKs or from leukocyte- and erythrocyte-depleted PLTs were Trizol extracted and further purified on silica matrix and then quality-checked using a Bioanalyzer 2100 and an RNA 6000 Nano kit (Agilent). .. On day 8 of the protocol, as described , PLTs from saline- and DT-treated mice were analyzed. (A) Representative profiles of RNA from in vitro differentiated MKs and PLTs from DT- or saline-treated animals. (B) Distribution of RIN and 28S/18S RNA values from retPLTs. (C) Percentage of retPLTs vs 28S/18S RNA ratio plot. (D) Percentage of retPLT vs platelet RNA content (fg/platelet).

Ethanol Precipitation:

Article Title: Proliferation Cycle Causes Age Dependent Mitochondrial Deficiencies and Contributes to the Aging of Stem Cells
Article Snippet: Amplification mix was extracted by phenol/chloroform to remove the proteins and concentrated with ethanol precipitation. .. In addition, 5.8 kb fragment was recovered, further digested with SspI overnight and analyzed on BioAnalyzer 2100 (Agilent, Savage, MD, USA) using a High Sensitivity DNA Kit (Agilent) following the manufacturer’s protocol.

Article Title: Next-Generation Sequencing-Based RiboMethSeq Protocol for Analysis of tRNA 2′-O-Methylation
Article Snippet: The reaction was stopped by ethanol precipitation using 3 M Na-acetate, pH 5.2 and glycoblue as a carrier in liquid nitrogen. .. The sizes of generated RNA fragments were assessed by capillary electrophoresis using a Small RNA chip on the Bioanalyzer 2100 (Agilent).

Next-Generation Sequencing:

Article Title: Towards Improving Embryo Prioritization: Parallel Next Generation Sequencing of DNA and RNA from a Single Trophectoderm Biopsy
Article Snippet: Biopsied cells lysed using the SMART-seq® protocol (I) (Takara BioInc, CA) yielded cDNA of high-quality and quantity, measured by BioAnalyzer 2100 (Agilent Technologies, CA) and fluorometer, respectively. .. The quantity of gDNA from this lysis approach was in the expected range (Table ), however, the integrity was affected which resulted in noisy low pass whole genome next generation sequencing (NGS) results using VeriSeq® kit (496,755 average reads, range of noise [derivative log ratio–DLR] 0.43–485.48) (Fig. ).

Article Title: Towards Improving Embryo Prioritization: Parallel Next Generation Sequencing of DNA and RNA from a Single Trophectoderm Biopsy
Article Snippet: .. The resulting amplified gDNA was sequenced using Nextera XT® library preparation for NGS following the VeriSeq® protocol, described in detail below (Illumina, CA). cDNA quality was assessed using BioAnalyzer 2100 (Agilent Technologies, CA). .. Table presents the results for chromosomal aberrations and sequencing quality metrics from VeriSeq® sequencing of gDNA obtained through SurePlex® kit using lysis with I. SMART-seq® (Takara BioInc, CA) or II.

Incubation:

Article Title: Time-Dependent Decay of mRNA and Ribosomal RNA during Platelet Aging and Its Correlation with Translation Activity
Article Snippet: Paragraph title: Supporting Information Increased MK density after recovery. Ultrastructure of TObright platelets. Specificity of TO and Y10b labeling. Phenotype of platelets incubated in vitro . Control of leukocyte and erythrocyte depletion. Quality of RNA extracted from in vitro -differentiated MKs and control or retPLTs. ... RNAs from in vitro -differentiated MKs or from leukocyte- and erythrocyte-depleted PLTs were Trizol extracted and further purified on silica matrix and then quality-checked using a Bioanalyzer 2100 and an RNA 6000 Nano kit (Agilent).

Spectrophotometry:

Article Title: Preservation of Multiple Mammalian Tissues to Maximize Science Return from Ground Based and Spaceflight Experiments
Article Snippet: RNA concentration and purity were assessed using a NanoDrop 2000 UV-Vis Spectrophotometer (Thermo Fisher Scientific, Waltham, MA). .. RNA quality was measured by calculating the RNA integrity number (RIN) using the Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA), where a RIN of 10 is considered intact and 0 totally degraded RNA [ , ].

Article Title: Small RNA Sequencing for Profiling MicroRNAs in Long-Term Preserved Formalin-Fixed and Paraffin-Embedded Non-Small Cell Lung Cancer Tumor Specimens
Article Snippet: RNA was quantified via absorbance spectrophotometry on a Nanodrop 2000 instrument (Thermo Scientific, Waltham, USA) and via RiboGreen dye fluorescence on a Qubit fluorometer (Life Technologies, Carlsbad, USA). .. Integrity of RNA was assessed using electrophoresis on Eukaryote Total RNA Pico chip on Bioanalyzer 2100 (Agilent, Santa Clara, USA).

Produced:

Article Title: Proliferation Cycle Causes Age Dependent Mitochondrial Deficiencies and Contributes to the Aging of Stem Cells
Article Snippet: The RCA amplification usually produced a band at 20 kb and a large amount of amplified DNA stuck in the well ( B). .. In addition, 5.8 kb fragment was recovered, further digested with SspI overnight and analyzed on BioAnalyzer 2100 (Agilent, Savage, MD, USA) using a High Sensitivity DNA Kit (Agilent) following the manufacturer’s protocol.

Article Title: Towards Improving Embryo Prioritization: Parallel Next Generation Sequencing of DNA and RNA from a Single Trophectoderm Biopsy
Article Snippet: .. The TE transcriptome obtained with PGT-AT was of high quality All tested samples produced sufficient quantity and high-quality cDNA for RNAseq, as per BioAnalyzer 2100 (Agilent Technologies, CA) (see Supplementary Fig. ), enabling us to conduct gene expression profiling. ..

Concentration Assay:

Article Title: Preservation of Multiple Mammalian Tissues to Maximize Science Return from Ground Based and Spaceflight Experiments
Article Snippet: RNA concentration and purity were assessed using a NanoDrop 2000 UV-Vis Spectrophotometer (Thermo Fisher Scientific, Waltham, MA). .. RNA quality was measured by calculating the RNA integrity number (RIN) using the Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA), where a RIN of 10 is considered intact and 0 totally degraded RNA [ , ].

Staining:

Article Title: Time-Dependent Decay of mRNA and Ribosomal RNA during Platelet Aging and Its Correlation with Translation Activity
Article Snippet: Gates corresponding to platelets (P), erythrocytes (E) and leukocytes (L), as defined before depletion (upper row), were applied to the other staining combinations after depletion (lower rows). .. RNAs from in vitro -differentiated MKs or from leukocyte- and erythrocyte-depleted PLTs were Trizol extracted and further purified on silica matrix and then quality-checked using a Bioanalyzer 2100 and an RNA 6000 Nano kit (Agilent).

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    Agilent technologies 2100 bioanalyzer
    Postfixation processing of an RNA 50mer with excess formaldehyde removed. Gel-simulated image (Agilent <t>2100</t> <t>Bioanalyzer)</t> of the RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. A: Lane L: RNA ladder. Lane 1: Non–formaldehyde-treated
    2100 Bioanalyzer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 148 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies dna high sensitivity kit
    Analysis of plasma <t>cfDNA</t> obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent <t>DNA</t> high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.
    Dna High Sensitivity Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies bioanalyzer 2100 instrument
    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent <t>Bioanalyzer</t> 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.
    Bioanalyzer 2100 Instrument, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Postfixation processing of an RNA 50mer with excess formaldehyde removed. Gel-simulated image (Agilent 2100 Bioanalyzer) of the RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. A: Lane L: RNA ladder. Lane 1: Non–formaldehyde-treated

    Journal: The Journal of Molecular Diagnostics : JMD

    Article Title: The Effect of Formaldehyde Fixation on RNA

    doi: 10.1016/j.jmoldx.2011.01.010

    Figure Lengend Snippet: Postfixation processing of an RNA 50mer with excess formaldehyde removed. Gel-simulated image (Agilent 2100 Bioanalyzer) of the RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. A: Lane L: RNA ladder. Lane 1: Non–formaldehyde-treated

    Article Snippet: The compositions of the total HeLa RNA and RNA 50mer preparations were characterized by capillary electrophoresis using a 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA).

    Techniques:

    Effects of fixation and postfixation processing on total cellular RNA: gel-simulated image (Agilent 2100 Bioanalyzer) of total cellular RNA fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. Lane L: RNA ladder. Lane 1: Native non–formaldehyde-treated

    Journal: The Journal of Molecular Diagnostics : JMD

    Article Title: The Effect of Formaldehyde Fixation on RNA

    doi: 10.1016/j.jmoldx.2011.01.010

    Figure Lengend Snippet: Effects of fixation and postfixation processing on total cellular RNA: gel-simulated image (Agilent 2100 Bioanalyzer) of total cellular RNA fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. Lane L: RNA ladder. Lane 1: Native non–formaldehyde-treated

    Article Snippet: The compositions of the total HeLa RNA and RNA 50mer preparations were characterized by capillary electrophoresis using a 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA).

    Techniques:

    Effects of buffer composition on postfixation processing of an RNA 50mer: gel-simulated image (Agilent 2100 Bioanalyzer). Lane L: RNA ladder. Lane 1: Native RNA 50mer. Lane 2: RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. Excess formaldehyde

    Journal: The Journal of Molecular Diagnostics : JMD

    Article Title: The Effect of Formaldehyde Fixation on RNA

    doi: 10.1016/j.jmoldx.2011.01.010

    Figure Lengend Snippet: Effects of buffer composition on postfixation processing of an RNA 50mer: gel-simulated image (Agilent 2100 Bioanalyzer). Lane L: RNA ladder. Lane 1: Native RNA 50mer. Lane 2: RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. Excess formaldehyde

    Article Snippet: The compositions of the total HeLa RNA and RNA 50mer preparations were characterized by capillary electrophoresis using a 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA).

    Techniques:

    Postformaldehyde fixation processing of an RNA 50mer. Gel-simulated image (Agilent 2100 Bioanalyzer) of the RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours and then heated at 70°C for 30 minutes, where indicated, in the following

    Journal: The Journal of Molecular Diagnostics : JMD

    Article Title: The Effect of Formaldehyde Fixation on RNA

    doi: 10.1016/j.jmoldx.2011.01.010

    Figure Lengend Snippet: Postformaldehyde fixation processing of an RNA 50mer. Gel-simulated image (Agilent 2100 Bioanalyzer) of the RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours and then heated at 70°C for 30 minutes, where indicated, in the following

    Article Snippet: The compositions of the total HeLa RNA and RNA 50mer preparations were characterized by capillary electrophoresis using a 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA).

    Techniques:

    Effects of fixation and postfixation processing on total HeLa RNA. Electropherograms (Agilent 2100 Bioanalyzer) of total cellular RNA fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. FU denotes fluorescence units. A: Non–formaldehyde-treated

    Journal: The Journal of Molecular Diagnostics : JMD

    Article Title: The Effect of Formaldehyde Fixation on RNA

    doi: 10.1016/j.jmoldx.2011.01.010

    Figure Lengend Snippet: Effects of fixation and postfixation processing on total HeLa RNA. Electropherograms (Agilent 2100 Bioanalyzer) of total cellular RNA fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. FU denotes fluorescence units. A: Non–formaldehyde-treated

    Article Snippet: The compositions of the total HeLa RNA and RNA 50mer preparations were characterized by capillary electrophoresis using a 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA).

    Techniques: Fluorescence

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques: