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Agilent technologies bioanalyzer 2100 system
Bioanalyzer 2100 System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 18 article reviews
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bioanalyzer 2100 system - by Bioz Stars, 2020-01
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Amplification:

Article Title: The RNA binding protein SORBS2 suppresses metastatic colonization of ovarian cancer by stabilizing tumor-suppressive immunomodulatory transcripts
Article Snippet: Libraries were controlled for quality and quantified using the BioAnalyzer 2100 system (Agilent Technologies, Inc., USA). .. Libraries (10 pM) were denatured as single-stranded DNA molecules, captured on Illumina flow cells, amplified in situ as clusters and finally sequenced for 150 cycles on an Illumina HiSeq Sequencer according to the manufacturer’s instructions at Cloudseq, Shanghai in China.

Article Title: A major gene controls mimicry and crypsis in butterflies and moths
Article Snippet: RNA quantification was performed with a Nanodrop spectrophotometer, and the RNA integrity was assessed using the Bioanalyzer 2100 system (Agilent). .. If a product amplified within 45 cycles then the RNA sample was re-treated with DNase.

Article Title: NuRD-interacting protein ZFP296 regulates genome-wide NuRD localization and differentiation of mouse embryonic stem cells
Article Snippet: 5 ng ChIP DNA was used as input, with NEXTflex adapters (Bioo Scientific) and 10 cycles of PCR amplification. .. Size-selected samples were analyzed for purity using a High Sensitivity DNA Chip on a Bioanalyzer 2100 system (Agilent).

Synthesized:

Article Title: Transcriptomic Responses of Skeletal Muscle to Acute Exercise in Diabetic Goto-Kakizaki Rats
Article Snippet: Then RNA integrity and concentration were assessed by the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 System (Agilent Technologies, Santa Clara, CA, United States). .. The library fragments were purified with QIAquick PCR Kits (Qiagen, Germany) following first-strand and second-strand cDNA synthesized.

Construct:

Article Title: The RNA binding protein SORBS2 suppresses metastatic colonization of ovarian cancer by stabilizing tumor-suppressive immunomodulatory transcripts
Article Snippet: RNA libraries were constructed by using rRNA-depleted RNAs with TruSeq Stranded Total RNA Library Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions. .. Libraries were controlled for quality and quantified using the BioAnalyzer 2100 system (Agilent Technologies, Inc., USA).

Real-time Polymerase Chain Reaction:

Article Title: UBL3 modification influences protein sorting to small extracellular vesicles
Article Snippet: The purified RNA solution was measured by a Bioanalyzer 2100 system (Agilent, 2100 Expert Ver. .. For real-time qPCR analysis, the purified RNA solution (40%) was reverse-transcribed into complementary DNA (cDNA), and 10% of the resulting cDNA solution for each miRNA was used in real-time qPCR analysis.

Microarray:

Article Title: Thymosin α1 represents a potential potent single molecule-based therapy for cystic fibrosis
Article Snippet: Paragraph title: Preparation of RNA and microarray hybridization ... Integrity of total RNA was evaluated using the Agilent Bioanalyzer 2100 system (Agilent Technologies) and was within RNA integrity number 7 to 9 and thus considered suitable for further processing.

Random Hexamer Labeling:

Article Title: A major gene controls mimicry and crypsis in butterflies and moths
Article Snippet: RNA quantification was performed with a Nanodrop spectrophotometer, and the RNA integrity was assessed using the Bioanalyzer 2100 system (Agilent). .. Single stranded cDNA was synthesised using BioScript MMLV Reverse Transcriptase (Bioline Reagents Ltd.) with random hexamer (N6) primers and 1µg of template RNA from each sample in a 20 µl reaction volume following the manufacturer’s protocol.

Expressing:

Article Title: A major gene controls mimicry and crypsis in butterflies and moths
Article Snippet: 5’ RACE, RT-PCR and qRT-PCR All tissues used for gene expression analyses were dissected from individuals from captive stocks derived from wild caught individuals of various races of Hm (aglaope, amaryllis, melpomene, rosina, plesseni, malleti ) and F2 individuals from a Hm rosina (female) x Hm melpomene (male) cross. .. RNA quantification was performed with a Nanodrop spectrophotometer, and the RNA integrity was assessed using the Bioanalyzer 2100 system (Agilent).

BIA-KA:

Article Title: UBL3 modification influences protein sorting to small extracellular vesicles
Article Snippet: The purified RNA solution was measured by a Bioanalyzer 2100 system (Agilent, 2100 Expert Ver. .. Protein concentrations of the serum, plasma, and cell lysates were measured by BCA (Thermo).

Derivative Assay:

Article Title: A major gene controls mimicry and crypsis in butterflies and moths
Article Snippet: 5’ RACE, RT-PCR and qRT-PCR All tissues used for gene expression analyses were dissected from individuals from captive stocks derived from wild caught individuals of various races of Hm (aglaope, amaryllis, melpomene, rosina, plesseni, malleti ) and F2 individuals from a Hm rosina (female) x Hm melpomene (male) cross. .. RNA quantification was performed with a Nanodrop spectrophotometer, and the RNA integrity was assessed using the Bioanalyzer 2100 system (Agilent).

Hybridization:

Article Title: Thymosin α1 represents a potential potent single molecule-based therapy for cystic fibrosis
Article Snippet: Paragraph title: Preparation of RNA and microarray hybridization ... Integrity of total RNA was evaluated using the Agilent Bioanalyzer 2100 system (Agilent Technologies) and was within RNA integrity number 7 to 9 and thus considered suitable for further processing.

Transfection:

Article Title: The RNA binding protein SORBS2 suppresses metastatic colonization of ovarian cancer by stabilizing tumor-suppressive immunomodulatory transcripts
Article Snippet: RIP sequencing Cell lysates of A2780s ovarian cancer cells transiently transfected with Flag-SORBS2 plasmid were used for the RNA immunoprecipitation assay as described previously [ ]. .. Libraries were controlled for quality and quantified using the BioAnalyzer 2100 system (Agilent Technologies, Inc., USA).

Sequencing:

Article Title: The RNA binding protein SORBS2 suppresses metastatic colonization of ovarian cancer by stabilizing tumor-suppressive immunomodulatory transcripts
Article Snippet: Paragraph title: RIP sequencing ... Libraries were controlled for quality and quantified using the BioAnalyzer 2100 system (Agilent Technologies, Inc., USA).

Article Title: Spectrin-based membrane skeleton supports ciliogenesis
Article Snippet: Paragraph title: RNA extraction, library preparation, and sequencing ... The RNA quality was assessed on the Agilent Bioanalyzer 2100 system.

Article Title: N4-Cytosine DNA Methylation Is Involved in the Maintenance of Genomic Stability in Deinococcus radiodurans
Article Snippet: Paragraph title: RNA Isolation and Sequencing (RNA-Seq) ... RNA concentration and RNA integrity were assessed using an RNA Nano 6000 Assay kit and Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, United States).

Article Title: NuRD-interacting protein ZFP296 regulates genome-wide NuRD localization and differentiation of mouse embryonic stem cells
Article Snippet: Paragraph title: Illumina high-throughput sequencing and data analysis ... Size-selected samples were analyzed for purity using a High Sensitivity DNA Chip on a Bioanalyzer 2100 system (Agilent).

Article Title: Transcriptomic Responses of Skeletal Muscle to Acute Exercise in Diabetic Goto-Kakizaki Rats
Article Snippet: Paragraph title: RNA Isolation, Library Preparation, and Sequencing ... Then RNA integrity and concentration were assessed by the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 System (Agilent Technologies, Santa Clara, CA, United States).

Article Title: An ErbB2/c-Src axis links bioenergetics with PRC2 translation to drive epigenetic reprogramming and mammary tumorigenesis
Article Snippet: RNA integrity was then assessed using the Bioanalyzer 2100 system (Agilent). .. In total 3 μg of RNA per sample was used to generate sequencing libraries using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (New England Biolabs), following the manufacturer’s recommendations, and index codes were added to attribute sequences to each sample.

Article Title: Metatranscriptomics of the Hu sheep rumen microbiome reveals novel cellulases
Article Snippet: Paragraph title: RNA isolation and metatranscriptomic sequencing ... The RNA integrity was evaluated using the RNA Nano 6000 Assay Kit and the Bioanalyzer 2100 system (Agilent Technologies, CA, USA).

Immunoprecipitation:

Article Title: The RNA binding protein SORBS2 suppresses metastatic colonization of ovarian cancer by stabilizing tumor-suppressive immunomodulatory transcripts
Article Snippet: RNA was extracted using Trizol following the manufacturer’s instructions (Invitrogen). rRNAs were removed from the immunoprecipitated RNA and input RNA samples by using Ribo-Zero™ rRNA Removal Kit (Illumina, San Diego, CA, USA). .. Libraries were controlled for quality and quantified using the BioAnalyzer 2100 system (Agilent Technologies, Inc., USA).

Generated:

Article Title: Spectrin-based membrane skeleton supports ciliogenesis
Article Snippet: The RNA quality was assessed on the Agilent Bioanalyzer 2100 system. .. A total of 150-bp paired-end reads were generated.

Article Title: Thymosin α1 represents a potential potent single molecule-based therapy for cystic fibrosis
Article Snippet: Integrity of total RNA was evaluated using the Agilent Bioanalyzer 2100 system (Agilent Technologies) and was within RNA integrity number 7 to 9 and thus considered suitable for further processing. .. Images were processed and cell intensity files (CEL files) were generated in the GeneChip Command Console Software (Affymetrix).

Article Title: N4-Cytosine DNA Methylation Is Involved in the Maintenance of Genomic Stability in Deinococcus radiodurans
Article Snippet: RNA concentration and RNA integrity were assessed using an RNA Nano 6000 Assay kit and Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, United States). .. The library preparations were sequenced on an Illumina Hiseq platform and paired-end reads were generated.

Article Title: Metatranscriptomics of the Hu sheep rumen microbiome reveals novel cellulases
Article Snippet: The RNA integrity was evaluated using the RNA Nano 6000 Assay Kit and the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). .. Then, one sequencing library was generated for each sample using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations.

Reverse Transcription Polymerase Chain Reaction:

Article Title: A major gene controls mimicry and crypsis in butterflies and moths
Article Snippet: Paragraph title: 5’ RACE, RT-PCR and qRT-PCR ... RNA quantification was performed with a Nanodrop spectrophotometer, and the RNA integrity was assessed using the Bioanalyzer 2100 system (Agilent).

Injection:

Article Title: Molecular parallelism in fast-twitch muscle proteins in echolocating mammals
Article Snippet: The bats were euthanized through an overdose of Narcoren [Merial GmbH, solution contained pentobarbital (400 mg/kg)] injected intraperitoneally. .. RNA integrity was assessed using a Bioanalyzer 2100 system (Agilent Technologies), and samples with RNA integrity number (RIN; a measurement of RNA degradation) > 7 were selected for RNA-seq.

ChIP-sequencing:

Article Title: NuRD-interacting protein ZFP296 regulates genome-wide NuRD localization and differentiation of mouse embryonic stem cells
Article Snippet: Illumina high-throughput sequencing and data analysis ChIP-seq libraries were prepared using the Kapa Hyper Prep Kit for Illumina sequencing (Kapa Biosystems) according to the manufacturer’s protocol with the following modifications. .. Size-selected samples were analyzed for purity using a High Sensitivity DNA Chip on a Bioanalyzer 2100 system (Agilent).

RNA Sequencing Assay:

Article Title: N4-Cytosine DNA Methylation Is Involved in the Maintenance of Genomic Stability in Deinococcus radiodurans
Article Snippet: Paragraph title: RNA Isolation and Sequencing (RNA-Seq) ... RNA concentration and RNA integrity were assessed using an RNA Nano 6000 Assay kit and Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, United States).

Article Title: Molecular parallelism in fast-twitch muscle proteins in echolocating mammals
Article Snippet: .. RNA integrity was assessed using a Bioanalyzer 2100 system (Agilent Technologies), and samples with RNA integrity number (RIN; a measurement of RNA degradation) > 7 were selected for RNA-seq. .. An Illumina HiSeq 2500 instrument was used to generate 75–base pair (bp) paired-end reads with an insert size of approximately 250 bp.

Article Title: Transcriptomic Responses of Skeletal Muscle to Acute Exercise in Diabetic Goto-Kakizaki Rats
Article Snippet: RNA Isolation, Library Preparation, and Sequencing Totally, 24 gastrocnemius muscle samples (RNA from six animals in each subgroup) were prepared for RNA-Seq. .. Then RNA integrity and concentration were assessed by the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 System (Agilent Technologies, Santa Clara, CA, United States).

Article Title: An ErbB2/c-Src axis links bioenergetics with PRC2 translation to drive epigenetic reprogramming and mammary tumorigenesis
Article Snippet: Paragraph title: RNA-Seq ... RNA integrity was then assessed using the Bioanalyzer 2100 system (Agilent).

Magnetic Beads:

Article Title: Transcriptomic Responses of Skeletal Muscle to Acute Exercise in Diabetic Goto-Kakizaki Rats
Article Snippet: Then RNA integrity and concentration were assessed by the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 System (Agilent Technologies, Santa Clara, CA, United States). .. Briefly, we used poly-T oligo-attached magnetic beads to purify mRNA samples from total RNA, and divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (NEB, United States) to carry out fragmentation.

Article Title: An ErbB2/c-Src axis links bioenergetics with PRC2 translation to drive epigenetic reprogramming and mammary tumorigenesis
Article Snippet: RNA integrity was then assessed using the Bioanalyzer 2100 system (Agilent). .. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads.

Isolation:

Article Title: Identification of differentially expressed non-coding RNAs and mRNAs involved in Qi stagnation and blood stasis syndrome
Article Snippet: Paragraph title: RNA isolation and library preparation ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit for the Bioanalyzer 2100 system (cat. no. 5067-1511; Agilent Technologies, Inc., Santa Clara, CA, USA).

Article Title: UBL3 modification influences protein sorting to small extracellular vesicles
Article Snippet: Paragraph title: Isolation of EVs and RNA analyses ... The purified RNA solution was measured by a Bioanalyzer 2100 system (Agilent, 2100 Expert Ver.

Article Title: N4-Cytosine DNA Methylation Is Involved in the Maintenance of Genomic Stability in Deinococcus radiodurans
Article Snippet: Paragraph title: RNA Isolation and Sequencing (RNA-Seq) ... RNA concentration and RNA integrity were assessed using an RNA Nano 6000 Assay kit and Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, United States).

Article Title: Molecular parallelism in fast-twitch muscle proteins in echolocating mammals
Article Snippet: The amount of isolated total RNA was measured with the NanoDrop (Thermo Fisher Scientific), and approximately 15 μg of RNA was found in each sample. .. RNA integrity was assessed using a Bioanalyzer 2100 system (Agilent Technologies), and samples with RNA integrity number (RIN; a measurement of RNA degradation) > 7 were selected for RNA-seq.

Article Title: Transcriptomic Responses of Skeletal Muscle to Acute Exercise in Diabetic Goto-Kakizaki Rats
Article Snippet: Paragraph title: RNA Isolation, Library Preparation, and Sequencing ... Then RNA integrity and concentration were assessed by the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 System (Agilent Technologies, Santa Clara, CA, United States).

Article Title: A model of human lung fibrogenesis for the assessment of anti-fibrotic strategies in idiopathic pulmonary fibrosis
Article Snippet: RNA Extraction Tissue was placed into RNA later and stored at either +4 °C for 1 week or −20 °C for up to 1 month before isolation. .. The RNA concentration and quality was measured using the Bioanalyzer 2100 system (Agilent, CA, USA).

Article Title: An ErbB2/c-Src axis links bioenergetics with PRC2 translation to drive epigenetic reprogramming and mammary tumorigenesis
Article Snippet: RNA-Seq RNA was isolated from two independent NIC/c-Src +/+ and two NIC/c-Src L/L cell lines, each in biological duplicate, using an RNEasy mini kit (Qiagen, 74106) according to the manufacturer’s instructions. .. RNA integrity was then assessed using the Bioanalyzer 2100 system (Agilent).

Article Title: Metatranscriptomics of the Hu sheep rumen microbiome reveals novel cellulases
Article Snippet: Paragraph title: RNA isolation and metatranscriptomic sequencing ... The RNA integrity was evaluated using the RNA Nano 6000 Assay Kit and the Bioanalyzer 2100 system (Agilent Technologies, CA, USA).

Flow Cytometry:

Article Title: The RNA binding protein SORBS2 suppresses metastatic colonization of ovarian cancer by stabilizing tumor-suppressive immunomodulatory transcripts
Article Snippet: Libraries were controlled for quality and quantified using the BioAnalyzer 2100 system (Agilent Technologies, Inc., USA). .. Libraries (10 pM) were denatured as single-stranded DNA molecules, captured on Illumina flow cells, amplified in situ as clusters and finally sequenced for 150 cycles on an Illumina HiSeq Sequencer according to the manufacturer’s instructions at Cloudseq, Shanghai in China.

Purification:

Article Title: UBL3 modification influences protein sorting to small extracellular vesicles
Article Snippet: .. The purified RNA solution was measured by a Bioanalyzer 2100 system (Agilent, 2100 Expert Ver. ..

Article Title: Transcriptomic Responses of Skeletal Muscle to Acute Exercise in Diabetic Goto-Kakizaki Rats
Article Snippet: Then RNA integrity and concentration were assessed by the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 System (Agilent Technologies, Santa Clara, CA, United States). .. The library fragments were purified with QIAquick PCR Kits (Qiagen, Germany) following first-strand and second-strand cDNA synthesized.

Article Title: A model of human lung fibrogenesis for the assessment of anti-fibrotic strategies in idiopathic pulmonary fibrosis
Article Snippet: Total RNA was purified using the automated QIAcube with RNeasy Fibrosis Mini kit (Qiagen, CA, USA) according to the manufacturer’s instructions. .. The RNA concentration and quality was measured using the Bioanalyzer 2100 system (Agilent, CA, USA).

Article Title: An ErbB2/c-Src axis links bioenergetics with PRC2 translation to drive epigenetic reprogramming and mammary tumorigenesis
Article Snippet: RNA integrity was then assessed using the Bioanalyzer 2100 system (Agilent). .. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads.

Polymerase Chain Reaction:

Article Title: A major gene controls mimicry and crypsis in butterflies and moths
Article Snippet: RNA quantification was performed with a Nanodrop spectrophotometer, and the RNA integrity was assessed using the Bioanalyzer 2100 system (Agilent). .. Total RNA was thoroughly checked for DNA contamination by performing PCR for EF1α (using primers ef1-a_RT_for and ef1-a_RT_rev, ) with 0.5µl of RNA extract (50ng-1µg of RNA) in a 20µl reaction using a polymerase enzyme that is not functional with RNA template (BioScript, Bioline Reagents Ltd.).

Article Title: Transcriptomic Responses of Skeletal Muscle to Acute Exercise in Diabetic Goto-Kakizaki Rats
Article Snippet: Then RNA integrity and concentration were assessed by the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 System (Agilent Technologies, Santa Clara, CA, United States). .. The library fragments were purified with QIAquick PCR Kits (Qiagen, Germany) following first-strand and second-strand cDNA synthesized.

Quantitative RT-PCR:

Article Title: A major gene controls mimicry and crypsis in butterflies and moths
Article Snippet: Paragraph title: 5’ RACE, RT-PCR and qRT-PCR ... RNA quantification was performed with a Nanodrop spectrophotometer, and the RNA integrity was assessed using the Bioanalyzer 2100 system (Agilent).

Chromatin Immunoprecipitation:

Article Title: NuRD-interacting protein ZFP296 regulates genome-wide NuRD localization and differentiation of mouse embryonic stem cells
Article Snippet: .. Size-selected samples were analyzed for purity using a High Sensitivity DNA Chip on a Bioanalyzer 2100 system (Agilent). .. Samples were sequenced on an Illumina HiSeq2000 or NextSeq500.

RNA Extraction:

Article Title: Identification of differentially expressed non-coding RNAs and mRNAs involved in Qi stagnation and blood stasis syndrome
Article Snippet: RNA integrity was assessed using the RNA Nano 6000 Assay Kit for the Bioanalyzer 2100 system (cat. no. 5067-1511; Agilent Technologies, Inc., Santa Clara, CA, USA). .. After RNA extraction, the total RNA was digested with TruSeq Stranded Total RNA with Ribo-Zero Gold (cat. no. 15021048; Illumina, Inc., San Diego, CA, USA).

Article Title: Spectrin-based membrane skeleton supports ciliogenesis
Article Snippet: Paragraph title: RNA extraction, library preparation, and sequencing ... The RNA quality was assessed on the Agilent Bioanalyzer 2100 system.

Article Title: A model of human lung fibrogenesis for the assessment of anti-fibrotic strategies in idiopathic pulmonary fibrosis
Article Snippet: Paragraph title: RNA Extraction ... The RNA concentration and quality was measured using the Bioanalyzer 2100 system (Agilent, CA, USA).

Plasmid Preparation:

Article Title: The RNA binding protein SORBS2 suppresses metastatic colonization of ovarian cancer by stabilizing tumor-suppressive immunomodulatory transcripts
Article Snippet: RIP sequencing Cell lysates of A2780s ovarian cancer cells transiently transfected with Flag-SORBS2 plasmid were used for the RNA immunoprecipitation assay as described previously [ ]. .. Libraries were controlled for quality and quantified using the BioAnalyzer 2100 system (Agilent Technologies, Inc., USA).

Software:

Article Title: Thymosin α1 represents a potential potent single molecule-based therapy for cystic fibrosis
Article Snippet: Integrity of total RNA was evaluated using the Agilent Bioanalyzer 2100 system (Agilent Technologies) and was within RNA integrity number 7 to 9 and thus considered suitable for further processing. .. Images were processed and cell intensity files (CEL files) were generated in the GeneChip Command Console Software (Affymetrix).

Electrophoresis:

Article Title: Metatranscriptomics of the Hu sheep rumen microbiome reveals novel cellulases
Article Snippet: The quality of the RNA and removal of potential contaminating DNA were visually assessed using agarose (1%) gels electrophoresis. .. The RNA integrity was evaluated using the RNA Nano 6000 Assay Kit and the Bioanalyzer 2100 system (Agilent Technologies, CA, USA).

Functional Assay:

Article Title: A major gene controls mimicry and crypsis in butterflies and moths
Article Snippet: RNA quantification was performed with a Nanodrop spectrophotometer, and the RNA integrity was assessed using the Bioanalyzer 2100 system (Agilent). .. Total RNA was thoroughly checked for DNA contamination by performing PCR for EF1α (using primers ef1-a_RT_for and ef1-a_RT_rev, ) with 0.5µl of RNA extract (50ng-1µg of RNA) in a 20µl reaction using a polymerase enzyme that is not functional with RNA template (BioScript, Bioline Reagents Ltd.).

Selection:

Article Title: NuRD-interacting protein ZFP296 regulates genome-wide NuRD localization and differentiation of mouse embryonic stem cells
Article Snippet: Post-amplification clean-up was performed with QIAquick MinElute columns (Qiagen) and size selection was done with an E-gel (300 bp fragments) (Thermo Fisher Scientific). .. Size-selected samples were analyzed for purity using a High Sensitivity DNA Chip on a Bioanalyzer 2100 system (Agilent).

In Situ:

Article Title: The RNA binding protein SORBS2 suppresses metastatic colonization of ovarian cancer by stabilizing tumor-suppressive immunomodulatory transcripts
Article Snippet: Libraries were controlled for quality and quantified using the BioAnalyzer 2100 system (Agilent Technologies, Inc., USA). .. Libraries (10 pM) were denatured as single-stranded DNA molecules, captured on Illumina flow cells, amplified in situ as clusters and finally sequenced for 150 cycles on an Illumina HiSeq Sequencer according to the manufacturer’s instructions at Cloudseq, Shanghai in China.

Spectrophotometry:

Article Title: A major gene controls mimicry and crypsis in butterflies and moths
Article Snippet: .. RNA quantification was performed with a Nanodrop spectrophotometer, and the RNA integrity was assessed using the Bioanalyzer 2100 system (Agilent). .. Total RNA was thoroughly checked for DNA contamination by performing PCR for EF1α (using primers ef1-a_RT_for and ef1-a_RT_rev, ) with 0.5µl of RNA extract (50ng-1µg of RNA) in a 20µl reaction using a polymerase enzyme that is not functional with RNA template (BioScript, Bioline Reagents Ltd.).

Article Title: An ErbB2/c-Src axis links bioenergetics with PRC2 translation to drive epigenetic reprogramming and mammary tumorigenesis
Article Snippet: Degradation and contamination were initially assessed on 1% agarose gels and purity was determined using the NanoPhotometer® spectrophotometer (IMPLEN). .. RNA integrity was then assessed using the Bioanalyzer 2100 system (Agilent).

Article Title: Metatranscriptomics of the Hu sheep rumen microbiome reveals novel cellulases
Article Snippet: Then, the RNA concentrations were confirmed using a NanoPhotometer® spectrophotometer (IMPLEN, CA, USA), and the RNA was quantified using a Qubit® RNA Assay Kit with a Qubit® 2.0 Fluorometer (Life Technologies, CA, USA). .. The RNA integrity was evaluated using the RNA Nano 6000 Assay Kit and the Bioanalyzer 2100 system (Agilent Technologies, CA, USA).

Concentration Assay:

Article Title: N4-Cytosine DNA Methylation Is Involved in the Maintenance of Genomic Stability in Deinococcus radiodurans
Article Snippet: .. RNA concentration and RNA integrity were assessed using an RNA Nano 6000 Assay kit and Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, United States). ..

Article Title: Transcriptomic Responses of Skeletal Muscle to Acute Exercise in Diabetic Goto-Kakizaki Rats
Article Snippet: .. Then RNA integrity and concentration were assessed by the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 System (Agilent Technologies, Santa Clara, CA, United States). .. Sequencing libraries were prepared using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, United States) and index codes were added to attribute sequences to each sample.

Article Title: A model of human lung fibrogenesis for the assessment of anti-fibrotic strategies in idiopathic pulmonary fibrosis
Article Snippet: .. The RNA concentration and quality was measured using the Bioanalyzer 2100 system (Agilent, CA, USA). ..

Article Title: An ErbB2/c-Src axis links bioenergetics with PRC2 translation to drive epigenetic reprogramming and mammary tumorigenesis
Article Snippet: RNA concentration was measured using a Qubit® 2.0 Fluorometer (Life Technologies, CA, USA) according to the manufacturer’s instructions. .. RNA integrity was then assessed using the Bioanalyzer 2100 system (Agilent).

High Throughput Screening Assay:

Article Title: NuRD-interacting protein ZFP296 regulates genome-wide NuRD localization and differentiation of mouse embryonic stem cells
Article Snippet: Paragraph title: Illumina high-throughput sequencing and data analysis ... Size-selected samples were analyzed for purity using a High Sensitivity DNA Chip on a Bioanalyzer 2100 system (Agilent).

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    Agilent technologies 2100 bioanalyzer
    Postfixation processing of an RNA 50mer with excess formaldehyde removed. Gel-simulated image (Agilent <t>2100</t> <t>Bioanalyzer)</t> of the RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. A: Lane L: RNA ladder. Lane 1: Non–formaldehyde-treated
    2100 Bioanalyzer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 148 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2100 bioanalyzer/product/Agilent technologies
    Average 99 stars, based on 148 article reviews
    Price from $9.99 to $1999.99
    2100 bioanalyzer - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    99
    Agilent technologies dna high sensitivity kit
    Analysis of plasma <t>cfDNA</t> obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent <t>DNA</t> high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.
    Dna High Sensitivity Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna high sensitivity kit/product/Agilent technologies
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    dna high sensitivity kit - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    99
    Agilent technologies bioanalyzer 2100 instrument
    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent <t>Bioanalyzer</t> 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.
    Bioanalyzer 2100 Instrument, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bioanalyzer 2100 instrument/product/Agilent technologies
    Average 99 stars, based on 90 article reviews
    Price from $9.99 to $1999.99
    bioanalyzer 2100 instrument - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    Image Search Results


    Postfixation processing of an RNA 50mer with excess formaldehyde removed. Gel-simulated image (Agilent 2100 Bioanalyzer) of the RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. A: Lane L: RNA ladder. Lane 1: Non–formaldehyde-treated

    Journal: The Journal of Molecular Diagnostics : JMD

    Article Title: The Effect of Formaldehyde Fixation on RNA

    doi: 10.1016/j.jmoldx.2011.01.010

    Figure Lengend Snippet: Postfixation processing of an RNA 50mer with excess formaldehyde removed. Gel-simulated image (Agilent 2100 Bioanalyzer) of the RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. A: Lane L: RNA ladder. Lane 1: Non–formaldehyde-treated

    Article Snippet: The compositions of the total HeLa RNA and RNA 50mer preparations were characterized by capillary electrophoresis using a 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA).

    Techniques:

    Effects of fixation and postfixation processing on total cellular RNA: gel-simulated image (Agilent 2100 Bioanalyzer) of total cellular RNA fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. Lane L: RNA ladder. Lane 1: Native non–formaldehyde-treated

    Journal: The Journal of Molecular Diagnostics : JMD

    Article Title: The Effect of Formaldehyde Fixation on RNA

    doi: 10.1016/j.jmoldx.2011.01.010

    Figure Lengend Snippet: Effects of fixation and postfixation processing on total cellular RNA: gel-simulated image (Agilent 2100 Bioanalyzer) of total cellular RNA fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. Lane L: RNA ladder. Lane 1: Native non–formaldehyde-treated

    Article Snippet: The compositions of the total HeLa RNA and RNA 50mer preparations were characterized by capillary electrophoresis using a 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA).

    Techniques:

    Effects of buffer composition on postfixation processing of an RNA 50mer: gel-simulated image (Agilent 2100 Bioanalyzer). Lane L: RNA ladder. Lane 1: Native RNA 50mer. Lane 2: RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. Excess formaldehyde

    Journal: The Journal of Molecular Diagnostics : JMD

    Article Title: The Effect of Formaldehyde Fixation on RNA

    doi: 10.1016/j.jmoldx.2011.01.010

    Figure Lengend Snippet: Effects of buffer composition on postfixation processing of an RNA 50mer: gel-simulated image (Agilent 2100 Bioanalyzer). Lane L: RNA ladder. Lane 1: Native RNA 50mer. Lane 2: RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. Excess formaldehyde

    Article Snippet: The compositions of the total HeLa RNA and RNA 50mer preparations were characterized by capillary electrophoresis using a 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA).

    Techniques:

    Postformaldehyde fixation processing of an RNA 50mer. Gel-simulated image (Agilent 2100 Bioanalyzer) of the RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours and then heated at 70°C for 30 minutes, where indicated, in the following

    Journal: The Journal of Molecular Diagnostics : JMD

    Article Title: The Effect of Formaldehyde Fixation on RNA

    doi: 10.1016/j.jmoldx.2011.01.010

    Figure Lengend Snippet: Postformaldehyde fixation processing of an RNA 50mer. Gel-simulated image (Agilent 2100 Bioanalyzer) of the RNA 50mer fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours and then heated at 70°C for 30 minutes, where indicated, in the following

    Article Snippet: The compositions of the total HeLa RNA and RNA 50mer preparations were characterized by capillary electrophoresis using a 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA).

    Techniques:

    Effects of fixation and postfixation processing on total HeLa RNA. Electropherograms (Agilent 2100 Bioanalyzer) of total cellular RNA fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. FU denotes fluorescence units. A: Non–formaldehyde-treated

    Journal: The Journal of Molecular Diagnostics : JMD

    Article Title: The Effect of Formaldehyde Fixation on RNA

    doi: 10.1016/j.jmoldx.2011.01.010

    Figure Lengend Snippet: Effects of fixation and postfixation processing on total HeLa RNA. Electropherograms (Agilent 2100 Bioanalyzer) of total cellular RNA fixed in 5% buffered formaldehyde (pH 7.4) for 2 hours. FU denotes fluorescence units. A: Non–formaldehyde-treated

    Article Snippet: The compositions of the total HeLa RNA and RNA 50mer preparations were characterized by capillary electrophoresis using a 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA).

    Techniques: Fluorescence

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 22°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in K 3 EDTA tubes at days 0, 3, 7, 14 and 28. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored (at 22°C) in ProTeck tubes at days 0, 3, 7, 14 and 28. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 30°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 2, 3, 7 and 14. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 2, 3, 7 and 14. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques:

    Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Journal: PLoS ONE

    Article Title: A novel approach to stabilize fetal cell-free DNA fraction in maternal blood samples for extended period of time

    doi: 10.1371/journal.pone.0208508

    Figure Lengend Snippet: Analysis of plasma cfDNA obtained from one representative pregnant donor blood stored at 4°C using Agilent Bioanalyzer 2100 instrument and Agilent DNA high sensitivity Kit. A, Overlaid electropherograms of plasma cfDNA extracted from blood stored in K 3 EDTA tubes at days 0, 3, 7, 14 and 21. B, Overlaid electropherograms of plasma cfDNA extracted from blood stored in ProTeck tubes at days 0, 3, 7, 14 and 21. C, Bioanalyzer gel image for blood stored in K 3 EDTA tubes. D, Bioanalyzer gel image for blood stored in ProTeck tubes. Cell-free DNA obtained from 3 pregnant donors were analyzed. This figure shows only the results of one representative pregnant donor.

    Article Snippet: Concentrated maternal cfDNA was analyzed by Agilent Bioanalyzer 2100 instrument and Agilent DNA High Sensitivity Kit following manufacturer’s recommended protocol.

    Techniques: