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Agilent technologies bioanalyzer 2100 system
First-round (unbarcoded) PCR product size composition measurement using microfluidic electrophoresis. The figure plots fragment sizes (calculated based on migration times relative to those of standards) and fluorescence intensity (FU) of first-round PCR products (see cycling conditions in Methods or Supplementary Fig. 2 legend) measured with the Agilent <t>Bioanalyzer</t> 2100 System. The first peak represents primer polymerization that is removed in subsequent gel excision/re-solubilization steps. The second peak matches expectations for the multi-target GLST product (164 – 204 bp). Special thanks to Craig Lapsley at the Wellcome Centre for Molecular Parasitology in Glasgow for generating this data.
Bioanalyzer 2100 System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 996 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bioanalyzer 2100 system/product/Agilent technologies
Average 92 stars, based on 996 article reviews
Price from $9.99 to $1999.99
bioanalyzer 2100 system - by Bioz Stars, 2020-07
92/100 stars

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1) Product Images from "Genome-wide locus sequence typing (GLST) of eukaryotic pathogens"

Article Title: Genome-wide locus sequence typing (GLST) of eukaryotic pathogens

Journal: bioRxiv

doi: 10.1101/2020.03.24.003590

First-round (unbarcoded) PCR product size composition measurement using microfluidic electrophoresis. The figure plots fragment sizes (calculated based on migration times relative to those of standards) and fluorescence intensity (FU) of first-round PCR products (see cycling conditions in Methods or Supplementary Fig. 2 legend) measured with the Agilent Bioanalyzer 2100 System. The first peak represents primer polymerization that is removed in subsequent gel excision/re-solubilization steps. The second peak matches expectations for the multi-target GLST product (164 – 204 bp). Special thanks to Craig Lapsley at the Wellcome Centre for Molecular Parasitology in Glasgow for generating this data.
Figure Legend Snippet: First-round (unbarcoded) PCR product size composition measurement using microfluidic electrophoresis. The figure plots fragment sizes (calculated based on migration times relative to those of standards) and fluorescence intensity (FU) of first-round PCR products (see cycling conditions in Methods or Supplementary Fig. 2 legend) measured with the Agilent Bioanalyzer 2100 System. The first peak represents primer polymerization that is removed in subsequent gel excision/re-solubilization steps. The second peak matches expectations for the multi-target GLST product (164 – 204 bp). Special thanks to Craig Lapsley at the Wellcome Centre for Molecular Parasitology in Glasgow for generating this data.

Techniques Used: Polymerase Chain Reaction, Electrophoresis, Migration, Fluorescence

Final (barcoded) GLST pool size composition measurement using microfluidic electrophoresis. The figure plots fragment sizes (calculated based on migration times relative to those of standards) and fluorescence intensity (FU) of the final GLST pool measured with the Agilent Bioanalyzer 2100 System. The large peak matches expectations for the multi-target GLST product pool (224 – 264 bp). Left and right peaks labelled in green and purple represent standards of known size. A small non-target peak remaining near 151 bp encourages improvement of prior size selection steps. Special thanks to Julie Galbraith at Glasgow Polyomics for generating this data.
Figure Legend Snippet: Final (barcoded) GLST pool size composition measurement using microfluidic electrophoresis. The figure plots fragment sizes (calculated based on migration times relative to those of standards) and fluorescence intensity (FU) of the final GLST pool measured with the Agilent Bioanalyzer 2100 System. The large peak matches expectations for the multi-target GLST product pool (224 – 264 bp). Left and right peaks labelled in green and purple represent standards of known size. A small non-target peak remaining near 151 bp encourages improvement of prior size selection steps. Special thanks to Julie Galbraith at Glasgow Polyomics for generating this data.

Techniques Used: Electrophoresis, Migration, Fluorescence, Selection

Related Articles

Polymerase Chain Reaction:

Article Title: Genome-wide locus sequence typing (GLST) of eukaryotic pathogens
Article Snippet: .. First-round PCR products were electrophoresed in 0.8% agarose gel to separate target bands (mode =164 nt) from primer polymers quantified with the Agilent Bioanalyzer 2100 System (see 78 nt primer peak in ). .. Excised target bands were resolubilized with the PureLink Quick Gel Extraction Kit (Invitrogen) to create input for subsequent barcoding PCR.

Article Title: Systematic Analysis of Non-coding RNAs Involved in the Angora Rabbit (Oryctolagus cuniculus) Hair Follicle Cycle by RNA Sequencing
Article Snippet: .. Finally, the PCR products were purified (AMPure XP system) and the library quality was assessed with the Agilent Bioanalyzer 2100 system. .. Library Construction for Small RNA Sequencing A total amount of 3 μg of RNA per sample was used as input material for the small RNA library.

Article Title: CBX2 Regulates Proliferation and Apoptosis via the Phosphorylation of YAP in Hepatocellular Carcinoma
Article Snippet: .. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system. .. Clustering and sequencing (Novogene Experimental Department): The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumia) according to the manufacturer's instructions.

Agarose Gel Electrophoresis:

Article Title: Genome-wide locus sequence typing (GLST) of eukaryotic pathogens
Article Snippet: .. First-round PCR products were electrophoresed in 0.8% agarose gel to separate target bands (mode =164 nt) from primer polymers quantified with the Agilent Bioanalyzer 2100 System (see 78 nt primer peak in ). .. Excised target bands were resolubilized with the PureLink Quick Gel Extraction Kit (Invitrogen) to create input for subsequent barcoding PCR.

Purification:

Article Title: Systematic Analysis of Non-coding RNAs Involved in the Angora Rabbit (Oryctolagus cuniculus) Hair Follicle Cycle by RNA Sequencing
Article Snippet: .. Finally, the PCR products were purified (AMPure XP system) and the library quality was assessed with the Agilent Bioanalyzer 2100 system. .. Library Construction for Small RNA Sequencing A total amount of 3 μg of RNA per sample was used as input material for the small RNA library.

Article Title: CBX2 Regulates Proliferation and Apoptosis via the Phosphorylation of YAP in Hepatocellular Carcinoma
Article Snippet: .. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system. .. Clustering and sequencing (Novogene Experimental Department): The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumia) according to the manufacturer's instructions.

Article Title: A central role for P2X7 receptors in human microglia
Article Snippet: .. Products were purified using AMPure XP system (Beckman Coulter, Beverly, USA) and quantified using the Agilent high-sensitivity DNA assay on the Agilent Bioanalyzer 2100 system. .. Patch clamp electrophysiology We studied both attached and detached microglia.

Article Title: Integration of small RNAs and transcriptome sequencing uncovers a complex regulatory network during vernalization and heading stages of orchardgrass (Dactylis glomerata L.)
Article Snippet: .. Products were purified using the AMPure XP system and library quality was assessed on Agilent Bioanalyzer 2100 system. .. Transcriptome sequencing was performed on an Illumina HiSeqTM 2000 platform.

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  • 91
    Agilent technologies dna chips
    Amplification results using the proposed <t>DNA</t> extraction method (on-chip- electrophoresis results). The size of PCR products are 79–115 bp ( <t>CSRM60</t> ), 100–124 bp ( INRA035 ). Left to right: L, ladder; 1, sample washing water control ( CSRM60 ); 2, extraction solution (without hair shaft) control ( CSRM60 ); 3, ddH 2 O control ( CSRM60 ); 4, DNA from hair shafts of pure bred Luxi cattle ( CSRM60 ); 5, DNA from liver of beef cattle ( CSRM60 ); 6, sample washing water control ( INRA035 ); 7, extraction solution (without hair shaft) control ( INRA035 ); 8, ddH 2 O control ( INRA035 ); 9, DNA from hair shafts of pure bred Luxi cattle ( INRA035 ); 10, DNA from liver of beef cattle ( INRA035 ).
    Dna Chips, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna chips/product/Agilent technologies
    Average 91 stars, based on 15 article reviews
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    dna chips - by Bioz Stars, 2020-07
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    99
    Agilent technologies agilent 2100 bioanalyzer
    Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using <t>Agilent</t> 2100 <t>Bioanalyzer</t> with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.
    Agilent 2100 Bioanalyzer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 6798 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agilent 2100 bioanalyzer/product/Agilent technologies
    Average 99 stars, based on 6798 article reviews
    Price from $9.99 to $1999.99
    agilent 2100 bioanalyzer - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    Amplification results using the proposed DNA extraction method (on-chip- electrophoresis results). The size of PCR products are 79–115 bp ( CSRM60 ), 100–124 bp ( INRA035 ). Left to right: L, ladder; 1, sample washing water control ( CSRM60 ); 2, extraction solution (without hair shaft) control ( CSRM60 ); 3, ddH 2 O control ( CSRM60 ); 4, DNA from hair shafts of pure bred Luxi cattle ( CSRM60 ); 5, DNA from liver of beef cattle ( CSRM60 ); 6, sample washing water control ( INRA035 ); 7, extraction solution (without hair shaft) control ( INRA035 ); 8, ddH 2 O control ( INRA035 ); 9, DNA from hair shafts of pure bred Luxi cattle ( INRA035 ); 10, DNA from liver of beef cattle ( INRA035 ).

    Journal: PLoS ONE

    Article Title: A Simple Method to Extract DNA from Hair Shafts Using Enzymatic Laundry Powder

    doi: 10.1371/journal.pone.0069588

    Figure Lengend Snippet: Amplification results using the proposed DNA extraction method (on-chip- electrophoresis results). The size of PCR products are 79–115 bp ( CSRM60 ), 100–124 bp ( INRA035 ). Left to right: L, ladder; 1, sample washing water control ( CSRM60 ); 2, extraction solution (without hair shaft) control ( CSRM60 ); 3, ddH 2 O control ( CSRM60 ); 4, DNA from hair shafts of pure bred Luxi cattle ( CSRM60 ); 5, DNA from liver of beef cattle ( CSRM60 ); 6, sample washing water control ( INRA035 ); 7, extraction solution (without hair shaft) control ( INRA035 ); 8, ddH 2 O control ( INRA035 ); 9, DNA from hair shafts of pure bred Luxi cattle ( INRA035 ); 10, DNA from liver of beef cattle ( INRA035 ).

    Article Snippet: Then, amplification products (locus CSRM60 and INRA035 ) were pipette onto DNA Chips (on-chip-electrophoresis, Agilent DNA 1000 Kit, for use with the Agilent 2100 bioanalyzer) and operated as its Guide described, while the others were visualized under UV light on 1.2% agarose gels by staining with GeneGreen (Tiangen Biotech (Beijing) Co., Ltd.).

    Techniques: Amplification, DNA Extraction, Chromatin Immunoprecipitation, Electrophoresis, Polymerase Chain Reaction

    Comparison of amplification results of DNA extracted from hair shafts using the proposed method and that extracted from liver using commercial Genomic DNA Purification Kit (on-chip-electrophoresis results). The above panel is INRA035 comparison result, sample 6 is amplification result of hair shaft DNA from pure bred Luxi cattle and sample 3 is amplification result of liver DNA from beef cattle; The below panel is CSRM60 comparison result, sample 2 is amplification result of hair shaft DNA from pure bred Luxi cattle and sample 11 is amplification result of liver DNA from beef cattle.

    Journal: PLoS ONE

    Article Title: A Simple Method to Extract DNA from Hair Shafts Using Enzymatic Laundry Powder

    doi: 10.1371/journal.pone.0069588

    Figure Lengend Snippet: Comparison of amplification results of DNA extracted from hair shafts using the proposed method and that extracted from liver using commercial Genomic DNA Purification Kit (on-chip-electrophoresis results). The above panel is INRA035 comparison result, sample 6 is amplification result of hair shaft DNA from pure bred Luxi cattle and sample 3 is amplification result of liver DNA from beef cattle; The below panel is CSRM60 comparison result, sample 2 is amplification result of hair shaft DNA from pure bred Luxi cattle and sample 11 is amplification result of liver DNA from beef cattle.

    Article Snippet: Then, amplification products (locus CSRM60 and INRA035 ) were pipette onto DNA Chips (on-chip-electrophoresis, Agilent DNA 1000 Kit, for use with the Agilent 2100 bioanalyzer) and operated as its Guide described, while the others were visualized under UV light on 1.2% agarose gels by staining with GeneGreen (Tiangen Biotech (Beijing) Co., Ltd.).

    Techniques: Amplification, DNA Purification, Chromatin Immunoprecipitation, Electrophoresis

    Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using Agilent 2100 Bioanalyzer with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.

    Journal: BMC Research Notes

    Article Title: Ensuring good quality rna for quantitative real-time pcr isolated from renal proximal tubular cells using laser capture microdissection

    doi: 10.1186/1756-0500-7-62

    Figure Lengend Snippet: Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using Agilent 2100 Bioanalyzer with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.

    Article Snippet: RNA integrity number (RIN) RIN was measured using Agilent 2100 Bioanalyzer with Agilent RNA 6000 Pico Kit.

    Techniques: Concentration Assay, Produced

    Analyses of the various antibody formats ( A ) To assess the purity and confirm the size of each reagent, the various antibody formats were analyzed by Agilent 2100 bioanalyzer. Proteins were assessed under non-reduced (NR) and reduced conditions (R). 1, IgG; 2, heavy chain; 3, light chain; 4, F(ab)’ 2 ; 5, VH-CH1-hinge; 6, monovalent antibody; 7, hinge-Fc; 8, Fab; 9, light chain and VH-CH1. ( B ) The binding of Hu 15C1 (black circles), monovalent Hu 15C1 (gray triangles), F(ab)’ 2 Hu 15C1 (open circles) and Fab Hu 15C1 (open diamonds) to TLR4 was analyzed by competitive ELISA. To compare the different antibody formats, the same number of binding site was used, i.e., the molar concentration of monovalent and Fab is twice the molar concentration of IgG and F(ab)’ 2 . Results are normalized and expressed as mean ± SD of duplicates. An F test was used to compare the fitted curves of different groups. ns: not significant.

    Journal: mAbs

    Article Title: Maximizing the potency of an anti-TLR4 monoclonal antibody by exploiting proximity to Fcγ receptors

    doi: 10.4161/19420862.2014.975098

    Figure Lengend Snippet: Analyses of the various antibody formats ( A ) To assess the purity and confirm the size of each reagent, the various antibody formats were analyzed by Agilent 2100 bioanalyzer. Proteins were assessed under non-reduced (NR) and reduced conditions (R). 1, IgG; 2, heavy chain; 3, light chain; 4, F(ab)’ 2 ; 5, VH-CH1-hinge; 6, monovalent antibody; 7, hinge-Fc; 8, Fab; 9, light chain and VH-CH1. ( B ) The binding of Hu 15C1 (black circles), monovalent Hu 15C1 (gray triangles), F(ab)’ 2 Hu 15C1 (open circles) and Fab Hu 15C1 (open diamonds) to TLR4 was analyzed by competitive ELISA. To compare the different antibody formats, the same number of binding site was used, i.e., the molar concentration of monovalent and Fab is twice the molar concentration of IgG and F(ab)’ 2 . Results are normalized and expressed as mean ± SD of duplicates. An F test was used to compare the fitted curves of different groups. ns: not significant.

    Article Snippet: The purity and chain composition of each reagent was assessed using an Agilent 2100 bioanalyzer ( ).

    Techniques: Binding Assay, Competitive ELISA, Concentration Assay

    Long-term storage effects on RNA integrity. RIN values for RNA samples isolated from Tempus tubes stored at –80°C until analyzed by Agilent 2100 Bioanalyzer. RIN values for adult blood samples (n = 15 Tempus tubes/year) and RIN values for cord blood samples (n = 6 Tempus tubes/year). Bars represent means ± SE. The average RIN values for adult and cord blood samples were 7.6 ± 0.5 and 7.7 ± 0.7, respectively, and no significant long-term storage related effects on RNA integrity were observed.

    Journal: BMC Research Notes

    Article Title: Long-term storage of blood RNA collected in RNA stabilizing Tempus tubes in a large biobank – evaluation of RNA quality and stability

    doi: 10.1186/1756-0500-7-633

    Figure Lengend Snippet: Long-term storage effects on RNA integrity. RIN values for RNA samples isolated from Tempus tubes stored at –80°C until analyzed by Agilent 2100 Bioanalyzer. RIN values for adult blood samples (n = 15 Tempus tubes/year) and RIN values for cord blood samples (n = 6 Tempus tubes/year). Bars represent means ± SE. The average RIN values for adult and cord blood samples were 7.6 ± 0.5 and 7.7 ± 0.7, respectively, and no significant long-term storage related effects on RNA integrity were observed.

    Article Snippet: The RNA integrity was assessed by an Agilent 2100 Bioanalyzer using the Eukaryote total RNA 6000 Nano LabChip kit and Eukaryote total RNA Nano assay according to the manufacturer’s instructions (Agilent Technologies, Palo Alto, CA).

    Techniques: Isolation