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Agilent technologies bioanalyser 2100
Quality of root-extracted RNA. The analysis of the total RNA extracted from inoculated roots was performed using an Agilent Bioanalyser 2100. One microlitre of RQ1 DNase-treated total RNA was analysed for each sample. The days post-inoculation with  Gaeumannomyces graminis  var.  tritici  ( Ggt ) are indicated. M, RNA size marker; lanes 1, 3, 6, 9, RNA from uninfected wheat roots; lanes 2, 4, 7, 10, RNA from wheat roots infected with  Ggt ; lanes 5, 8, 11, RNA from wheat roots infected with  Ggt  and Pf29Arp; lane 12, RNA from soil-borne pathogenic fungus IV-26/00.
Bioanalyser 2100, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bioanalyser 2100/product/Agilent technologies
Average 93 stars, based on 97 article reviews
Price from $9.99 to $1999.99
bioanalyser 2100 - by Bioz Stars, 2020-07
93/100 stars

Images

1) Product Images from "The biocontrol bacterium Pseudomonas fluorescens Pf29Arp strain affects the pathogenesis-related gene expression of the take-all fungus Gaeumannomyces graminis var. tritici on wheat roots"

Article Title: The biocontrol bacterium Pseudomonas fluorescens Pf29Arp strain affects the pathogenesis-related gene expression of the take-all fungus Gaeumannomyces graminis var. tritici on wheat roots

Journal: Molecular Plant Pathology

doi: 10.1111/j.1364-3703.2011.00715.x

Quality of root-extracted RNA. The analysis of the total RNA extracted from inoculated roots was performed using an Agilent Bioanalyser 2100. One microlitre of RQ1 DNase-treated total RNA was analysed for each sample. The days post-inoculation with  Gaeumannomyces graminis  var.  tritici  ( Ggt ) are indicated. M, RNA size marker; lanes 1, 3, 6, 9, RNA from uninfected wheat roots; lanes 2, 4, 7, 10, RNA from wheat roots infected with  Ggt ; lanes 5, 8, 11, RNA from wheat roots infected with  Ggt  and Pf29Arp; lane 12, RNA from soil-borne pathogenic fungus IV-26/00.
Figure Legend Snippet: Quality of root-extracted RNA. The analysis of the total RNA extracted from inoculated roots was performed using an Agilent Bioanalyser 2100. One microlitre of RQ1 DNase-treated total RNA was analysed for each sample. The days post-inoculation with Gaeumannomyces graminis var. tritici ( Ggt ) are indicated. M, RNA size marker; lanes 1, 3, 6, 9, RNA from uninfected wheat roots; lanes 2, 4, 7, 10, RNA from wheat roots infected with Ggt ; lanes 5, 8, 11, RNA from wheat roots infected with Ggt and Pf29Arp; lane 12, RNA from soil-borne pathogenic fungus IV-26/00.

Techniques Used: Marker, Infection

2) Product Images from "The biocontrol bacterium Pseudomonas fluorescens Pf29Arp strain affects the pathogenesis-related gene expression of the take-all fungus Gaeumannomyces graminis var. tritici on wheat roots"

Article Title: The biocontrol bacterium Pseudomonas fluorescens Pf29Arp strain affects the pathogenesis-related gene expression of the take-all fungus Gaeumannomyces graminis var. tritici on wheat roots

Journal: Molecular Plant Pathology

doi: 10.1111/j.1364-3703.2011.00715.x

Quality of root-extracted RNA. The analysis of the total RNA extracted from inoculated roots was performed using an Agilent Bioanalyser 2100. One microlitre of RQ1 DNase-treated total RNA was analysed for each sample. The days post-inoculation with  Gaeumannomyces graminis  var.  tritici  ( Ggt ) are indicated. M, RNA size marker; lanes 1, 3, 6, 9, RNA from uninfected wheat roots; lanes 2, 4, 7, 10, RNA from wheat roots infected with  Ggt ; lanes 5, 8, 11, RNA from wheat roots infected with  Ggt  and Pf29Arp; lane 12, RNA from soil-borne pathogenic fungus IV-26/00.
Figure Legend Snippet: Quality of root-extracted RNA. The analysis of the total RNA extracted from inoculated roots was performed using an Agilent Bioanalyser 2100. One microlitre of RQ1 DNase-treated total RNA was analysed for each sample. The days post-inoculation with Gaeumannomyces graminis var. tritici ( Ggt ) are indicated. M, RNA size marker; lanes 1, 3, 6, 9, RNA from uninfected wheat roots; lanes 2, 4, 7, 10, RNA from wheat roots infected with Ggt ; lanes 5, 8, 11, RNA from wheat roots infected with Ggt and Pf29Arp; lane 12, RNA from soil-borne pathogenic fungus IV-26/00.

Techniques Used: Marker, Infection

3) Product Images from "An optimized method for the extraction of bacterial mRNA from plant roots infected with Escherichia coli O157:H7"

Article Title: An optimized method for the extraction of bacterial mRNA from plant roots infected with Escherichia coli O157:H7

Journal: Frontiers in Microbiology

doi: 10.3389/fmicb.2014.00286

Total RNA from plant root extractions by BPEX and Bead/SDS/phenol methods . Intact plant roots were suspended in 20 ml of bacterial inoculum (OD 600 of 0.2) for 2 h at 18°C. Total RNA was extracted using either the BPEX or the Bead/SDS/phenol method and RNA quality was assessed by spectroscopy on a Bioanalyser 2100 machine (Agilent). (A) A montage of an electropherograph of the total RNA levels following extraction of inoculated plant material. The lanes show RNA from an in vitro culture of E. coli O157:H7 isolate Sakai only (lane S); uninfected lettuce roots (lane L); E. coli O157:H7 Sakai infected lettuce roots (Lane 1, 306 ng μl - 1 ; Lane 4, 119 ng μl - 1 ; Lane 5, 195 ng μl - 1 ); and E. coli O157:H7 Sakai infected spinach roots (Lane 2, 248 ng μl - 1 ; Lane 3, 301 ng μl - 1 ; Lane 6, 141 ng μl - 1 ). Lanes 1, 2, and 3 show extraction using the BPEX method, and Lanes 4, 5, and 6 using the Bead method. The samples were run alongside a commercial RNA transcript ladder (0.2, 0.5, 1.0, 2.0, 4.0, and 6.0 kb, Agilent); the 16S and 23S (bacterial), and 18S and 28S (plant) r RNA bands are indicated. Electropherogram of E. coli O157:H7 Sakai infected lettuce roots extracted with (B) the BPEX method (sample derived from lane 1) or with (C) the novel Bead/SDS/phenol method (sample derived from lane 4). The uninfected spinach control was similar to lettuce (lane L) (not shown).
Figure Legend Snippet: Total RNA from plant root extractions by BPEX and Bead/SDS/phenol methods . Intact plant roots were suspended in 20 ml of bacterial inoculum (OD 600 of 0.2) for 2 h at 18°C. Total RNA was extracted using either the BPEX or the Bead/SDS/phenol method and RNA quality was assessed by spectroscopy on a Bioanalyser 2100 machine (Agilent). (A) A montage of an electropherograph of the total RNA levels following extraction of inoculated plant material. The lanes show RNA from an in vitro culture of E. coli O157:H7 isolate Sakai only (lane S); uninfected lettuce roots (lane L); E. coli O157:H7 Sakai infected lettuce roots (Lane 1, 306 ng μl - 1 ; Lane 4, 119 ng μl - 1 ; Lane 5, 195 ng μl - 1 ); and E. coli O157:H7 Sakai infected spinach roots (Lane 2, 248 ng μl - 1 ; Lane 3, 301 ng μl - 1 ; Lane 6, 141 ng μl - 1 ). Lanes 1, 2, and 3 show extraction using the BPEX method, and Lanes 4, 5, and 6 using the Bead method. The samples were run alongside a commercial RNA transcript ladder (0.2, 0.5, 1.0, 2.0, 4.0, and 6.0 kb, Agilent); the 16S and 23S (bacterial), and 18S and 28S (plant) r RNA bands are indicated. Electropherogram of E. coli O157:H7 Sakai infected lettuce roots extracted with (B) the BPEX method (sample derived from lane 1) or with (C) the novel Bead/SDS/phenol method (sample derived from lane 4). The uninfected spinach control was similar to lettuce (lane L) (not shown).

Techniques Used: Spectroscopy, In Vitro, Infection, Derivative Assay

Related Articles

Sequencing:

Article Title: Bacterial community structure and function shift along a successional series of tidal flats in the Yellow River Delta
Article Snippet: .. Prior to sequencing, all amplicons were assessed for fragment size distribution and DNA concentration using a Bioanalyser 2100 (Agilent Technologies, USA). .. The samples were adjusted to a final concentration of 10 pM and were attached to the surface of Ion Sphere Particles (ISPs) using an Ion Xpress Template 100 kit (Life Technologies, USA) according to the manufacturer’s instructions.

Concentration Assay:

Article Title: Bacterial community structure and function shift along a successional series of tidal flats in the Yellow River Delta
Article Snippet: .. Prior to sequencing, all amplicons were assessed for fragment size distribution and DNA concentration using a Bioanalyser 2100 (Agilent Technologies, USA). .. The samples were adjusted to a final concentration of 10 pM and were attached to the surface of Ion Sphere Particles (ISPs) using an Ion Xpress Template 100 kit (Life Technologies, USA) according to the manufacturer’s instructions.

Article Title: An optimized method for the extraction of bacterial mRNA from plant roots infected with Escherichia coli O157:H7
Article Snippet: .. Total RNA concentration was determined using a NanoDrop (Wilmington, USA) spectrophotometer and the relative proportions of ribosomal RNA determined using a BioAnalyser 2100 (Agilent Technologies, Santa Clara, USA), for both methods. .. c DNA Synthesis and qPCR Conditions c DNA was transcribed from 1 μg total RNA using Superscript II (Invitrogen, Carlsbad, USA) following the random primer protocol.

other:

Article Title: Myometrial Transcriptional Signatures of Human Parturition
Article Snippet: The quantity and quality of cDNA libraries were also tested by a Qubit fluorimeter and Bioanalyser 2100.

Spectrophotometry:

Article Title: An optimized method for the extraction of bacterial mRNA from plant roots infected with Escherichia coli O157:H7
Article Snippet: .. Total RNA concentration was determined using a NanoDrop (Wilmington, USA) spectrophotometer and the relative proportions of ribosomal RNA determined using a BioAnalyser 2100 (Agilent Technologies, Santa Clara, USA), for both methods. .. c DNA Synthesis and qPCR Conditions c DNA was transcribed from 1 μg total RNA using Superscript II (Invitrogen, Carlsbad, USA) following the random primer protocol.

Article Title: Identification of unusual peptides with new Cys frameworks in the venom of the cold-water sea anemone Cnidopus japonicus
Article Snippet: .. The yield and purity were assessed using a Nanodrop ND-1000 spectrophotometer (Thermo Scientific, US), with the RNA integrity determined by the RNA integrity number using a Bioanalyser 2100 (Agilent Technologies, US). .. The PCR-based cDNA library was created following the instructions for the Mint-2 cDNA synthesis kit (Evrogen, Russia).

Hybridization:

Article Title: Systems biology approach to identify transcriptome reprogramming and candidate microRNA targets during the progression of polycystic kidney disease
Article Snippet: .. The integrity and purity of the mRNA samples were assessed prior to hybridization using Bioanalyser 2100 with mRNA Nanochips (Agilent Technologies). .. RNA hybridization was performed, and gene expression profiles were determined using Illumina Mouse Sentrix 6 version 2 Beadchips.

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    Agilent technologies agilent 2100 bioanalyser
    Isolation of cotton tissues from paraffin-embedded sections by laser microdissection (LMD). Isolation of cotton tissues from paraffin-embedded sections by laser microdissection (LMD). Sections before LMD (a, c, e) , and sections after LM (b, d, f) . The area selected for laser microdissection is outlined in green (a region near the damage caused by larvae feeding, which comprised the stamen tissue, viewed at a , c and d ) or blue (a region farther from the injured area, which comprised the carpel tissue, viewed at a , e , and f ). The assessment of extracted RNA integrity from the stamen and carpel are shown in g and h , respectively. Electropherograms were obtained with an <t>Agilent</t> 2100 <t>Bioanalyser.</t> Open and closed arrowheads indicate the 18S and 28S ribosomal RNA peaks, respectively. RNA quality is expressed as the RNA integrity number (RIN). Scale bars = 100 μm.
    Agilent 2100 Bioanalyser, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 533 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agilent 2100 bioanalyser/product/Agilent technologies
    Average 94 stars, based on 533 article reviews
    Price from $9.99 to $1999.99
    agilent 2100 bioanalyser - by Bioz Stars, 2020-07
    94/100 stars
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    Isolation of cotton tissues from paraffin-embedded sections by laser microdissection (LMD). Isolation of cotton tissues from paraffin-embedded sections by laser microdissection (LMD). Sections before LMD (a, c, e) , and sections after LM (b, d, f) . The area selected for laser microdissection is outlined in green (a region near the damage caused by larvae feeding, which comprised the stamen tissue, viewed at a , c and d ) or blue (a region farther from the injured area, which comprised the carpel tissue, viewed at a , e , and f ). The assessment of extracted RNA integrity from the stamen and carpel are shown in g and h , respectively. Electropherograms were obtained with an Agilent 2100 Bioanalyser. Open and closed arrowheads indicate the 18S and 28S ribosomal RNA peaks, respectively. RNA quality is expressed as the RNA integrity number (RIN). Scale bars = 100 μm.

    Journal: BMC Genomics

    Article Title: Transcriptome analysis of Gossypium hirsutum flower buds infested by cotton boll weevil (Anthonomus grandis) larvae

    doi: 10.1186/1471-2164-15-854

    Figure Lengend Snippet: Isolation of cotton tissues from paraffin-embedded sections by laser microdissection (LMD). Isolation of cotton tissues from paraffin-embedded sections by laser microdissection (LMD). Sections before LMD (a, c, e) , and sections after LM (b, d, f) . The area selected for laser microdissection is outlined in green (a region near the damage caused by larvae feeding, which comprised the stamen tissue, viewed at a , c and d ) or blue (a region farther from the injured area, which comprised the carpel tissue, viewed at a , e , and f ). The assessment of extracted RNA integrity from the stamen and carpel are shown in g and h , respectively. Electropherograms were obtained with an Agilent 2100 Bioanalyser. Open and closed arrowheads indicate the 18S and 28S ribosomal RNA peaks, respectively. RNA quality is expressed as the RNA integrity number (RIN). Scale bars = 100 μm.

    Article Snippet: The quality of total RNA extracted from LMD-collected tissues was assessed using an RNA 6000 Pico kit on the Agilent 2100 Bioanalyser (Agilent Technologies).

    Techniques: Isolation, Laser Capture Microdissection

    Quality of root-extracted RNA. The analysis of the total RNA extracted from inoculated roots was performed using an Agilent Bioanalyser 2100. One microlitre of RQ1 DNase-treated total RNA was analysed for each sample. The days post-inoculation with  Gaeumannomyces graminis  var.  tritici  ( Ggt ) are indicated. M, RNA size marker; lanes 1, 3, 6, 9, RNA from uninfected wheat roots; lanes 2, 4, 7, 10, RNA from wheat roots infected with  Ggt ; lanes 5, 8, 11, RNA from wheat roots infected with  Ggt  and Pf29Arp; lane 12, RNA from soil-borne pathogenic fungus IV-26/00.

    Journal: Molecular Plant Pathology

    Article Title: The biocontrol bacterium Pseudomonas fluorescens Pf29Arp strain affects the pathogenesis-related gene expression of the take-all fungus Gaeumannomyces graminis var. tritici on wheat roots

    doi: 10.1111/j.1364-3703.2011.00715.x

    Figure Lengend Snippet: Quality of root-extracted RNA. The analysis of the total RNA extracted from inoculated roots was performed using an Agilent Bioanalyser 2100. One microlitre of RQ1 DNase-treated total RNA was analysed for each sample. The days post-inoculation with Gaeumannomyces graminis var. tritici ( Ggt ) are indicated. M, RNA size marker; lanes 1, 3, 6, 9, RNA from uninfected wheat roots; lanes 2, 4, 7, 10, RNA from wheat roots infected with Ggt ; lanes 5, 8, 11, RNA from wheat roots infected with Ggt and Pf29Arp; lane 12, RNA from soil-borne pathogenic fungus IV-26/00.

    Article Snippet: RNA purity and quality were assessed with a Bioanalyser 2100 (Agilent) and quantified with a Nanodrop (Agilent).

    Techniques: Marker, Infection

    Murine CD4 + CD25 + Treg EVs contain different miRNA compared to control T cell EVs. ( A) dTreg and control FoxP3 - T cell EVs (n = 3 per group) isolated by ExoQuick-TC were lysed and total RNA was purified and assessed using NanoDrop™ spectrophotometer and Agilent 2100 Bioanalyser. EVs miRNA was reverse transcribed into cDNA and pre-amplified before miRNA detection using QuantStudio™ 12 K Flex Real-Time PCR System with the OpenArray® Platform. The volcano plot shows the relation between the p-value and the log fold change between FoxP3 − T cell and Treg EV miRNA expression levels. The blue line indicates the inverse log 10 of the p-value = 0.05. ( B ) Bar graphs (mean + SEM) showing the relative quantity of miR-142-3p, miR-150-5p, and miR-384-5p by control T cell EVs (Control EVs) and Treg EVs (Treg EVs) as measured by qPCR and normalised relative to RNU6-2. Data pooled from 3 individual experiments that were performed in triplicates. Statistical significance was determined using a two-tailed Student’s t-test where *p

    Journal: Scientific Reports

    Article Title: Regulatory T cell-derived extracellular vesicles modify dendritic cell function

    doi: 10.1038/s41598-018-24531-8

    Figure Lengend Snippet: Murine CD4 + CD25 + Treg EVs contain different miRNA compared to control T cell EVs. ( A) dTreg and control FoxP3 - T cell EVs (n = 3 per group) isolated by ExoQuick-TC were lysed and total RNA was purified and assessed using NanoDrop™ spectrophotometer and Agilent 2100 Bioanalyser. EVs miRNA was reverse transcribed into cDNA and pre-amplified before miRNA detection using QuantStudio™ 12 K Flex Real-Time PCR System with the OpenArray® Platform. The volcano plot shows the relation between the p-value and the log fold change between FoxP3 − T cell and Treg EV miRNA expression levels. The blue line indicates the inverse log 10 of the p-value = 0.05. ( B ) Bar graphs (mean + SEM) showing the relative quantity of miR-142-3p, miR-150-5p, and miR-384-5p by control T cell EVs (Control EVs) and Treg EVs (Treg EVs) as measured by qPCR and normalised relative to RNU6-2. Data pooled from 3 individual experiments that were performed in triplicates. Statistical significance was determined using a two-tailed Student’s t-test where *p

    Article Snippet: Total RNA profile was assessed using an Agilent 2100 Bioanalyser and total RNA nano chips and quantified by NanoDrop™ spectrophotometer (Thermo Fisher Scientific). cDNA fragments were synthesised using the ‘miScript RT Kit’ (Qiagen, Hilden, Germany), according to manufacturer’s protocols. qPCR reactions were set up with the miScript SYBR Green PCR Kit and primers specific for miR-125b-5p, miR-29a-3p, miR-182-5p, miR-142-3p, miR-150-5p, miR-384-5p, SCARNA17 and RNU6-2, as per manufacturer’s protocols and run on the Applied Biosystems™ ViiA™ 7 Real time PCR system.

    Techniques: Isolation, Purification, Spectrophotometry, Amplification, Real-time Polymerase Chain Reaction, Expressing, Two Tailed Test

    Capillary electrophoreses of IPCR (odd-numbered lanes) and PAI-PCR (even-numbered lanes) products amplified from DNA extracted from horse vermiform appendixes. The electrophoreses followed by the analysis of the electrophoregrams were automatically performed by using an Agilent 2100 Bioanalyser with a DNA 7500 LabChip® kit (TaKaRa-Bio). The results are shown as a gel-like image. L is the ladder obtained with DNA fragment size markers (bp). The green and pink peaks are the lowest 50 bp and highest 10 380 bp markers respectively. The substrates used for IPCR or PAI-PCR were BamHI-digested DNA (lanes 1 and 2), EcoRI-digested DNA (lanes 3 and 4), HindIII-digested DNA (lanes 5 and 6), PstI-digested DNA (lanes 7 and 8) and XbaI-digested DNA (lanes 9 and 10).

    Journal: Environmental Microbiology

    Article Title: Retrieval of entire genes from environmental DNA by inverse PCR with pre-amplification of target genes using primers containing locked nucleic acids

    doi: 10.1111/j.1462-2920.2007.01518.x

    Figure Lengend Snippet: Capillary electrophoreses of IPCR (odd-numbered lanes) and PAI-PCR (even-numbered lanes) products amplified from DNA extracted from horse vermiform appendixes. The electrophoreses followed by the analysis of the electrophoregrams were automatically performed by using an Agilent 2100 Bioanalyser with a DNA 7500 LabChip® kit (TaKaRa-Bio). The results are shown as a gel-like image. L is the ladder obtained with DNA fragment size markers (bp). The green and pink peaks are the lowest 50 bp and highest 10 380 bp markers respectively. The substrates used for IPCR or PAI-PCR were BamHI-digested DNA (lanes 1 and 2), EcoRI-digested DNA (lanes 3 and 4), HindIII-digested DNA (lanes 5 and 6), PstI-digested DNA (lanes 7 and 8) and XbaI-digested DNA (lanes 9 and 10).

    Article Snippet: Products were analysed with an Agilent 2100 Bioanalyser with a DNA7500 LabChip® kit (TaKaRa-Bio).

    Techniques: Polymerase Chain Reaction, Amplification

    Capillary electrophoreses of PAI-PCR products amplified from DNA extracted from 12 different termite gut samples. All DNA samples were digested with EcoRI, and an RCA primer [(A) Xyn-RC(LNA-Even); or (B) Xyn-RC(LNA-5′)] was used in the RCA reaction. Products were analysed with an Agilent 2100 Bioanalyser with a DNA 7500 LabChip® kit (TaKaRa-Bio).

    Journal: Environmental Microbiology

    Article Title: Retrieval of entire genes from environmental DNA by inverse PCR with pre-amplification of target genes using primers containing locked nucleic acids

    doi: 10.1111/j.1462-2920.2007.01518.x

    Figure Lengend Snippet: Capillary electrophoreses of PAI-PCR products amplified from DNA extracted from 12 different termite gut samples. All DNA samples were digested with EcoRI, and an RCA primer [(A) Xyn-RC(LNA-Even); or (B) Xyn-RC(LNA-5′)] was used in the RCA reaction. Products were analysed with an Agilent 2100 Bioanalyser with a DNA 7500 LabChip® kit (TaKaRa-Bio).

    Article Snippet: Products were analysed with an Agilent 2100 Bioanalyser with a DNA7500 LabChip® kit (TaKaRa-Bio).

    Techniques: Polymerase Chain Reaction, Amplification