Structured Review

Bio-Rad bio silect
Bio Silect, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 88/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bio silect/product/Bio-Rad
Average 88 stars, based on 22 article reviews
Price from $9.99 to $1999.99
bio silect - by Bioz Stars, 2021-01
88/100 stars

Images

Related Articles

High Performance Liquid Chromatography:

Article Title: Synthesis and Preclinical Evaluation of Bifunctional Ligands for Improved Chelation Chemistry of 90Y and 177Lu for Targeted Radioimmunotherapy
Article Snippet: .. Size-exclusion HPLC (SE-HPLC) chromatograms were obtained on Agilent 1200 equipped with a diode array detector and an in-line IN/US γ-Ram Model 2 radiodetector (Tampa, FL) fitted with Bio-Silect SEC 250-5 column (Bio-Rad, Hercules, CA), TSK-G3000PW column (Tosoh bioscience, King of Prussia, PA), or a BioSep-SEC S 3000 column (Phenomenex, Torrance, CA). ..

Flow Cytometry:

Article Title: Ex vivo analysis of human memory CD4 T cells specific for hepatitis C virus using MHC class II tetramers
Article Snippet: .. Complexes of DR and Alexa-488–labeled peptide were separated from unbound peptide using a Bio-Silect SEC 125 gel filtration column (Bio-Rad Laboratories Inc., Hercules, California, USA) run at a flow rate of 1 ml/min with PBS. .. The Alexa-488–labeled peptide was detected using an HPLC fluorescence detector with excitation and emission wavelengths set at 495 and 519 nm, respectively (Varian ProStar 363; Varian Analytical Instruments, Walnut Creek, California, USA).

Filtration:

Article Title: The Antibacterial Protein Lysozyme Identified as the Termite Egg Recognition Pheromone
Article Snippet: .. The lyophilised sample was redissolved in 50 µl of 50 mM phosphate buffer (pH 6.8) with 0.15 M NaCl and applied to a Bio-Silect SEC 125 Gel Filtration Column (7.8 mm×300 mm; BioRad Laboratories, CA) equilibrated with the same buffer. ..

Article Title: Setting of Methods for Analysis of Mucosal Antibodies in Seminal and Vaginal Fluids of HIV Seropositive Subjects from Cambodian and Italian Cohorts
Article Snippet: .. Antibody concentration, medium change and separation of IgA from IgM by gel filtration chromatography Immunoglobulin fractions containing IgG and IgA-IgM antibodies were concentrated on Amicon cartridges; the latter ones were charged on the Bio-Silect SEC 400–5 column (BioRad) to separe IgA from IgM antibodies. .. Columns were extensively washed with water and equilibrated with sterile PBS buffer (7 volumes; flow: 200 microL/min).

Article Title: The First Archaeal ATP-Dependent Glucokinase, from the Hyperthermophilic Crenarchaeon Aeropyrum pernix, Represents a Monomeric, Extremely Thermophilic ROK Glucokinase with Broad Hexose Specificity
Article Snippet: .. The apparent molecular mass of native ATP-GLK was determined by gel filtration and was 33 ± 3 kDa on Bio-Prep SE1000/17 (semipreparative grade; Bio-Rad) and 30 ± 3 kDa on Bio-Silect SEC 250-5 (analytical grade; Bio-Rad). .. SDS-PAGE revealed only one subunit with an apparent molecular mass of 36 kDa (Fig. ), indicating that ATP-GLK is a monomeric protein.

Article Title: Ex vivo analysis of human memory CD4 T cells specific for hepatitis C virus using MHC class II tetramers
Article Snippet: .. Complexes of DR and Alexa-488–labeled peptide were separated from unbound peptide using a Bio-Silect SEC 125 gel filtration column (Bio-Rad Laboratories Inc., Hercules, California, USA) run at a flow rate of 1 ml/min with PBS. .. The Alexa-488–labeled peptide was detected using an HPLC fluorescence detector with excitation and emission wavelengths set at 495 and 519 nm, respectively (Varian ProStar 363; Varian Analytical Instruments, Walnut Creek, California, USA).

Article Title: Purification, characterization, and molecular cloning of a novel amine:pyruvate transaminase from Vibrio fluvialis JS17.
Article Snippet: .. A transaminase from Vibrio fluvialis JS17 showing activity toward chiral amines was purified to homogeneity and its enzymatic properties were characterized. .. A transaminase from Vibrio fluvialis JS17 showing activity toward chiral amines was purified to homogeneity and its enzymatic properties were characterized.

Chromatography:

Article Title: Setting of Methods for Analysis of Mucosal Antibodies in Seminal and Vaginal Fluids of HIV Seropositive Subjects from Cambodian and Italian Cohorts
Article Snippet: .. Antibody concentration, medium change and separation of IgA from IgM by gel filtration chromatography Immunoglobulin fractions containing IgG and IgA-IgM antibodies were concentrated on Amicon cartridges; the latter ones were charged on the Bio-Silect SEC 400–5 column (BioRad) to separe IgA from IgM antibodies. .. Columns were extensively washed with water and equilibrated with sterile PBS buffer (7 volumes; flow: 200 microL/min).

Article Title: Purification, characterization, and molecular cloning of a novel amine:pyruvate transaminase from Vibrio fluvialis JS17.
Article Snippet: .. A transaminase from Vibrio fluvialis JS17 showing activity toward chiral amines was purified to homogeneity and its enzymatic properties were characterized. .. A transaminase from Vibrio fluvialis JS17 showing activity toward chiral amines was purified to homogeneity and its enzymatic properties were characterized.

Size-exclusion Chromatography:

Article Title: NMR Solution Structure of the Integral Membrane Enzyme DsbB - Functional Insights into DsbB Catalyzed Disulfide Bond Formation
Article Snippet: .. The oligomerization status of DsbB[CSSC] in DPC micelles was characterized by size exclusion chromatography using a Bio-Silect SEC 250-5 Column (Bio-Rad Laboratories) ( ). ..

Article Title: Preclinical characterisation of 111In-DTPA-trastuzumab
Article Snippet: .. The size exclusion column used was a Bio Silect SEC 250-5, 300 × 7.8 mm2 column from Bio-Rad Laboratories BV (Veenendaal, The Netherlands). ..

Article Title: The Antibacterial Protein Lysozyme Identified as the Termite Egg Recognition Pheromone
Article Snippet: .. The lyophilised sample was redissolved in 50 µl of 50 mM phosphate buffer (pH 6.8) with 0.15 M NaCl and applied to a Bio-Silect SEC 125 Gel Filtration Column (7.8 mm×300 mm; BioRad Laboratories, CA) equilibrated with the same buffer. ..

Article Title: Setting of Methods for Analysis of Mucosal Antibodies in Seminal and Vaginal Fluids of HIV Seropositive Subjects from Cambodian and Italian Cohorts
Article Snippet: .. Antibody concentration, medium change and separation of IgA from IgM by gel filtration chromatography Immunoglobulin fractions containing IgG and IgA-IgM antibodies were concentrated on Amicon cartridges; the latter ones were charged on the Bio-Silect SEC 400–5 column (BioRad) to separe IgA from IgM antibodies. .. Columns were extensively washed with water and equilibrated with sterile PBS buffer (7 volumes; flow: 200 microL/min).

Article Title: Synthesis and Preclinical Evaluation of Bifunctional Ligands for Improved Chelation Chemistry of 90Y and 177Lu for Targeted Radioimmunotherapy
Article Snippet: .. Size-exclusion HPLC (SE-HPLC) chromatograms were obtained on Agilent 1200 equipped with a diode array detector and an in-line IN/US γ-Ram Model 2 radiodetector (Tampa, FL) fitted with Bio-Silect SEC 250-5 column (Bio-Rad, Hercules, CA), TSK-G3000PW column (Tosoh bioscience, King of Prussia, PA), or a BioSep-SEC S 3000 column (Phenomenex, Torrance, CA). ..

Article Title: The First Archaeal ATP-Dependent Glucokinase, from the Hyperthermophilic Crenarchaeon Aeropyrum pernix, Represents a Monomeric, Extremely Thermophilic ROK Glucokinase with Broad Hexose Specificity
Article Snippet: .. The apparent molecular mass of native ATP-GLK was determined by gel filtration and was 33 ± 3 kDa on Bio-Prep SE1000/17 (semipreparative grade; Bio-Rad) and 30 ± 3 kDa on Bio-Silect SEC 250-5 (analytical grade; Bio-Rad). .. SDS-PAGE revealed only one subunit with an apparent molecular mass of 36 kDa (Fig. ), indicating that ATP-GLK is a monomeric protein.

Article Title: Ex vivo analysis of human memory CD4 T cells specific for hepatitis C virus using MHC class II tetramers
Article Snippet: .. Complexes of DR and Alexa-488–labeled peptide were separated from unbound peptide using a Bio-Silect SEC 125 gel filtration column (Bio-Rad Laboratories Inc., Hercules, California, USA) run at a flow rate of 1 ml/min with PBS. .. The Alexa-488–labeled peptide was detected using an HPLC fluorescence detector with excitation and emission wavelengths set at 495 and 519 nm, respectively (Varian ProStar 363; Varian Analytical Instruments, Walnut Creek, California, USA).

Purification:

Article Title: Purification, characterization, and molecular cloning of a novel amine:pyruvate transaminase from Vibrio fluvialis JS17.
Article Snippet: .. A transaminase from Vibrio fluvialis JS17 showing activity toward chiral amines was purified to homogeneity and its enzymatic properties were characterized. .. A transaminase from Vibrio fluvialis JS17 showing activity toward chiral amines was purified to homogeneity and its enzymatic properties were characterized.

Concentration Assay:

Article Title: Setting of Methods for Analysis of Mucosal Antibodies in Seminal and Vaginal Fluids of HIV Seropositive Subjects from Cambodian and Italian Cohorts
Article Snippet: .. Antibody concentration, medium change and separation of IgA from IgM by gel filtration chromatography Immunoglobulin fractions containing IgG and IgA-IgM antibodies were concentrated on Amicon cartridges; the latter ones were charged on the Bio-Silect SEC 400–5 column (BioRad) to separe IgA from IgM antibodies. .. Columns were extensively washed with water and equilibrated with sterile PBS buffer (7 volumes; flow: 200 microL/min).

Sequencing:

Article Title: Purification, characterization, and molecular cloning of a novel amine:pyruvate transaminase from Vibrio fluvialis JS17.
Article Snippet: .. A transaminase from Vibrio fluvialis JS17 showing activity toward chiral amines was purified to homogeneity and its enzymatic properties were characterized. .. A transaminase from Vibrio fluvialis JS17 showing activity toward chiral amines was purified to homogeneity and its enzymatic properties were characterized.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 85
    Bio-Rad bio silect 250
    SGF-2 is a 1.1-MDa heteromeric complex. A , chromatography chart of SGF-2 activity passed through a Bio-Silect 250 gel filtration column.  B , EMSA was performed using the E site probe, and each fraction passed through a Bio-Silect 250 column.  Source Q fr
    Bio Silect 250, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bio silect 250/product/Bio-Rad
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bio silect 250 - by Bioz Stars, 2021-01
    85/100 stars
      Buy from Supplier

    80
    Bio-Rad bio silect chromatography column
    Purification of the active component from pig brain cytosol. ( A ) Cibachrome blue 3GA: a step-eluted fraction from fast-flow <t>Q-Sepharose,</t> diluted to 50 mM salt, was passed over a 40 ml Cibachrome blue 3GA column, washed extensively, and eluted as shown in a continuous 25-300 μS salt gradient. Protein was monitored in line by absorbence at 280 nm, and salt concentration by conductivity, as shown. The activities of column fractions were assayed and plotted ( far left ordinate axis ) and the two activity peaks were pooled. ( B ) Phenyl–Sepharose: pooled fractions from A were applied to a 15-ml phenyl–Sepharose column and proteins eluted with a 300–25 μS salt gradient. ( C ) Q-Sepharose: pooled active fractions from B were diluted to 20 mM salt and chromatographed on a 5-ml Q-Sepharose column. Activity was eluted in a salt gradient to 200 μS. ( D ) 200 μl of the peak fraction from C was applied to a <t>Bio-Silect</t> gel filtration column with a rated separation range of 100–5 kD. The elution positions of molecular weight standards are indicated.
    Bio Silect Chromatography Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 80/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bio silect chromatography column/product/Bio-Rad
    Average 80 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bio silect chromatography column - by Bioz Stars, 2021-01
    80/100 stars
      Buy from Supplier

    88
    Bio-Rad bio silect
    Purification of the active component from pig brain cytosol. ( A ) Cibachrome blue 3GA: a step-eluted fraction from fast-flow <t>Q-Sepharose,</t> diluted to 50 mM salt, was passed over a 40 ml Cibachrome blue 3GA column, washed extensively, and eluted as shown in a continuous 25-300 μS salt gradient. Protein was monitored in line by absorbence at 280 nm, and salt concentration by conductivity, as shown. The activities of column fractions were assayed and plotted ( far left ordinate axis ) and the two activity peaks were pooled. ( B ) Phenyl–Sepharose: pooled fractions from A were applied to a 15-ml phenyl–Sepharose column and proteins eluted with a 300–25 μS salt gradient. ( C ) Q-Sepharose: pooled active fractions from B were diluted to 20 mM salt and chromatographed on a 5-ml Q-Sepharose column. Activity was eluted in a salt gradient to 200 μS. ( D ) 200 μl of the peak fraction from C was applied to a <t>Bio-Silect</t> gel filtration column with a rated separation range of 100–5 kD. The elution positions of molecular weight standards are indicated.
    Bio Silect, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 88/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bio silect/product/Bio-Rad
    Average 88 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    bio silect - by Bioz Stars, 2021-01
    88/100 stars
      Buy from Supplier

    N/A
    Pkg of 1 5 port 4 position solenoid valve 30 psi 2 bar limit
      Buy from Supplier

    Image Search Results


    SGF-2 is a 1.1-MDa heteromeric complex. A , chromatography chart of SGF-2 activity passed through a Bio-Silect 250 gel filtration column.  B , EMSA was performed using the E site probe, and each fraction passed through a Bio-Silect 250 column.  Source Q fr

    Journal: The Journal of Biological Chemistry

    Article Title: Silk Gland Factor-2, Involved in Fibroin Gene Transcription, Consists of LIM Homeodomain, LIM-interacting, and Single-stranded DNA-binding Proteins *

    doi: 10.1074/jbc.M113.514471

    Figure Lengend Snippet: SGF-2 is a 1.1-MDa heteromeric complex. A , chromatography chart of SGF-2 activity passed through a Bio-Silect 250 gel filtration column. B , EMSA was performed using the E site probe, and each fraction passed through a Bio-Silect 250 column. Source Q fr

    Article Snippet: The total eluate (0.15 mg, 2.1 ml) was dialyzed against TEMGTK100 and loaded onto a column of Bio-Silect 250 (Bio-Rad) equilibrated with TEMGTK100 .

    Techniques: Multiple Displacement Amplification, Chromatography, Activity Assay, Filtration

    Purification of the active component from pig brain cytosol. ( A ) Cibachrome blue 3GA: a step-eluted fraction from fast-flow Q-Sepharose, diluted to 50 mM salt, was passed over a 40 ml Cibachrome blue 3GA column, washed extensively, and eluted as shown in a continuous 25-300 μS salt gradient. Protein was monitored in line by absorbence at 280 nm, and salt concentration by conductivity, as shown. The activities of column fractions were assayed and plotted ( far left ordinate axis ) and the two activity peaks were pooled. ( B ) Phenyl–Sepharose: pooled fractions from A were applied to a 15-ml phenyl–Sepharose column and proteins eluted with a 300–25 μS salt gradient. ( C ) Q-Sepharose: pooled active fractions from B were diluted to 20 mM salt and chromatographed on a 5-ml Q-Sepharose column. Activity was eluted in a salt gradient to 200 μS. ( D ) 200 μl of the peak fraction from C was applied to a Bio-Silect gel filtration column with a rated separation range of 100–5 kD. The elution positions of molecular weight standards are indicated.

    Journal: The Journal of Cell Biology

    Article Title: Rho- and Rac-dependent Assembly of Focal Adhesion Complexes and Actin Filaments in Permeabilized Fibroblasts: An Essential Role for Ezrin/Radixin/Moesin Proteins

    doi:

    Figure Lengend Snippet: Purification of the active component from pig brain cytosol. ( A ) Cibachrome blue 3GA: a step-eluted fraction from fast-flow Q-Sepharose, diluted to 50 mM salt, was passed over a 40 ml Cibachrome blue 3GA column, washed extensively, and eluted as shown in a continuous 25-300 μS salt gradient. Protein was monitored in line by absorbence at 280 nm, and salt concentration by conductivity, as shown. The activities of column fractions were assayed and plotted ( far left ordinate axis ) and the two activity peaks were pooled. ( B ) Phenyl–Sepharose: pooled fractions from A were applied to a 15-ml phenyl–Sepharose column and proteins eluted with a 300–25 μS salt gradient. ( C ) Q-Sepharose: pooled active fractions from B were diluted to 20 mM salt and chromatographed on a 5-ml Q-Sepharose column. Activity was eluted in a salt gradient to 200 μS. ( D ) 200 μl of the peak fraction from C was applied to a Bio-Silect gel filtration column with a rated separation range of 100–5 kD. The elution positions of molecular weight standards are indicated.

    Article Snippet: For gel filtration, a 200-μl aliquot of the activity peak from the final Q-Sepharose column was applied to a 6-ml Bio-Silect chromatography column (Bio Rad Laboratories: rated fractionation range 100–5 kD) and protein was eluted in DK150, sampling 0.25-ml fractions.

    Techniques: Purification, Flow Cytometry, Concentration Assay, Activity Assay, Filtration, Molecular Weight