Structured Review

Bio-Rad bio silect chromatography column
Purification of the active component from pig brain cytosol. ( A ) Cibachrome blue 3GA: a step-eluted fraction from fast-flow <t>Q-Sepharose,</t> diluted to 50 mM salt, was passed over a 40 ml Cibachrome blue 3GA column, washed extensively, and eluted as shown in a continuous 25-300 μS salt gradient. Protein was monitored in line by absorbence at 280 nm, and salt concentration by conductivity, as shown. The activities of column fractions were assayed and plotted ( far left ordinate axis ) and the two activity peaks were pooled. ( B ) Phenyl–Sepharose: pooled fractions from A were applied to a 15-ml phenyl–Sepharose column and proteins eluted with a 300–25 μS salt gradient. ( C ) Q-Sepharose: pooled active fractions from B were diluted to 20 mM salt and chromatographed on a 5-ml Q-Sepharose column. Activity was eluted in a salt gradient to 200 μS. ( D ) 200 μl of the peak fraction from C was applied to a <t>Bio-Silect</t> gel filtration column with a rated separation range of 100–5 kD. The elution positions of molecular weight standards are indicated.
Bio Silect Chromatography Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 80/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bio silect chromatography column/product/Bio-Rad
Average 80 stars, based on 2 article reviews
Price from $9.99 to $1999.99
bio silect chromatography column - by Bioz Stars, 2020-09
80/100 stars

Related Products / Commonly Used Together

q-sepharose column

Images

1) Product Images from "Rho- and Rac-dependent Assembly of Focal Adhesion Complexes and Actin Filaments in Permeabilized Fibroblasts: An Essential Role for Ezrin/Radixin/Moesin Proteins "

Article Title: Rho- and Rac-dependent Assembly of Focal Adhesion Complexes and Actin Filaments in Permeabilized Fibroblasts: An Essential Role for Ezrin/Radixin/Moesin Proteins

Journal: The Journal of Cell Biology

doi:

Purification of the active component from pig brain cytosol. ( A ) Cibachrome blue 3GA: a step-eluted fraction from fast-flow Q-Sepharose, diluted to 50 mM salt, was passed over a 40 ml Cibachrome blue 3GA column, washed extensively, and eluted as shown in a continuous 25-300 μS salt gradient. Protein was monitored in line by absorbence at 280 nm, and salt concentration by conductivity, as shown. The activities of column fractions were assayed and plotted ( far left ordinate axis ) and the two activity peaks were pooled. ( B ) Phenyl–Sepharose: pooled fractions from A were applied to a 15-ml phenyl–Sepharose column and proteins eluted with a 300–25 μS salt gradient. ( C ) Q-Sepharose: pooled active fractions from B were diluted to 20 mM salt and chromatographed on a 5-ml Q-Sepharose column. Activity was eluted in a salt gradient to 200 μS. ( D ) 200 μl of the peak fraction from C was applied to a Bio-Silect gel filtration column with a rated separation range of 100–5 kD. The elution positions of molecular weight standards are indicated.
Figure Legend Snippet: Purification of the active component from pig brain cytosol. ( A ) Cibachrome blue 3GA: a step-eluted fraction from fast-flow Q-Sepharose, diluted to 50 mM salt, was passed over a 40 ml Cibachrome blue 3GA column, washed extensively, and eluted as shown in a continuous 25-300 μS salt gradient. Protein was monitored in line by absorbence at 280 nm, and salt concentration by conductivity, as shown. The activities of column fractions were assayed and plotted ( far left ordinate axis ) and the two activity peaks were pooled. ( B ) Phenyl–Sepharose: pooled fractions from A were applied to a 15-ml phenyl–Sepharose column and proteins eluted with a 300–25 μS salt gradient. ( C ) Q-Sepharose: pooled active fractions from B were diluted to 20 mM salt and chromatographed on a 5-ml Q-Sepharose column. Activity was eluted in a salt gradient to 200 μS. ( D ) 200 μl of the peak fraction from C was applied to a Bio-Silect gel filtration column with a rated separation range of 100–5 kD. The elution positions of molecular weight standards are indicated.

Techniques Used: Purification, Flow Cytometry, Concentration Assay, Activity Assay, Filtration, Molecular Weight

Related Articles

Filtration:

Article Title: Rho- and Rac-dependent Assembly of Focal Adhesion Complexes and Actin Filaments in Permeabilized Fibroblasts: An Essential Role for Ezrin/Radixin/Moesin Proteins
Article Snippet: .. For gel filtration, a 200-μl aliquot of the activity peak from the final Q-Sepharose column was applied to a 6-ml Bio-Silect chromatography column (Bio Rad Laboratories: rated fractionation range 100–5 kD) and protein was eluted in DK150, sampling 0.25-ml fractions. ..

Activity Assay:

Article Title: Rho- and Rac-dependent Assembly of Focal Adhesion Complexes and Actin Filaments in Permeabilized Fibroblasts: An Essential Role for Ezrin/Radixin/Moesin Proteins
Article Snippet: .. For gel filtration, a 200-μl aliquot of the activity peak from the final Q-Sepharose column was applied to a 6-ml Bio-Silect chromatography column (Bio Rad Laboratories: rated fractionation range 100–5 kD) and protein was eluted in DK150, sampling 0.25-ml fractions. ..

Fractionation:

Article Title: Rho- and Rac-dependent Assembly of Focal Adhesion Complexes and Actin Filaments in Permeabilized Fibroblasts: An Essential Role for Ezrin/Radixin/Moesin Proteins
Article Snippet: .. For gel filtration, a 200-μl aliquot of the activity peak from the final Q-Sepharose column was applied to a 6-ml Bio-Silect chromatography column (Bio Rad Laboratories: rated fractionation range 100–5 kD) and protein was eluted in DK150, sampling 0.25-ml fractions. ..

Chromatography:

Article Title: Rho- and Rac-dependent Assembly of Focal Adhesion Complexes and Actin Filaments in Permeabilized Fibroblasts: An Essential Role for Ezrin/Radixin/Moesin Proteins
Article Snippet: .. For gel filtration, a 200-μl aliquot of the activity peak from the final Q-Sepharose column was applied to a 6-ml Bio-Silect chromatography column (Bio Rad Laboratories: rated fractionation range 100–5 kD) and protein was eluted in DK150, sampling 0.25-ml fractions. ..

Sampling:

Article Title: Rho- and Rac-dependent Assembly of Focal Adhesion Complexes and Actin Filaments in Permeabilized Fibroblasts: An Essential Role for Ezrin/Radixin/Moesin Proteins
Article Snippet: .. For gel filtration, a 200-μl aliquot of the activity peak from the final Q-Sepharose column was applied to a 6-ml Bio-Silect chromatography column (Bio Rad Laboratories: rated fractionation range 100–5 kD) and protein was eluted in DK150, sampling 0.25-ml fractions. ..

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 80
    Bio-Rad bio silect chromatography column
    Purification of the active component from pig brain cytosol. ( A ) Cibachrome blue 3GA: a step-eluted fraction from fast-flow <t>Q-Sepharose,</t> diluted to 50 mM salt, was passed over a 40 ml Cibachrome blue 3GA column, washed extensively, and eluted as shown in a continuous 25-300 μS salt gradient. Protein was monitored in line by absorbence at 280 nm, and salt concentration by conductivity, as shown. The activities of column fractions were assayed and plotted ( far left ordinate axis ) and the two activity peaks were pooled. ( B ) Phenyl–Sepharose: pooled fractions from A were applied to a 15-ml phenyl–Sepharose column and proteins eluted with a 300–25 μS salt gradient. ( C ) Q-Sepharose: pooled active fractions from B were diluted to 20 mM salt and chromatographed on a 5-ml Q-Sepharose column. Activity was eluted in a salt gradient to 200 μS. ( D ) 200 μl of the peak fraction from C was applied to a <t>Bio-Silect</t> gel filtration column with a rated separation range of 100–5 kD. The elution positions of molecular weight standards are indicated.
    Bio Silect Chromatography Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 80/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bio silect chromatography column/product/Bio-Rad
    Average 80 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bio silect chromatography column - by Bioz Stars, 2020-09
    80/100 stars
      Buy from Supplier

    91
    Bio-Rad se hplc
    Purification of the active component from pig brain cytosol. ( A ) Cibachrome blue 3GA: a step-eluted fraction from fast-flow <t>Q-Sepharose,</t> diluted to 50 mM salt, was passed over a 40 ml Cibachrome blue 3GA column, washed extensively, and eluted as shown in a continuous 25-300 μS salt gradient. Protein was monitored in line by absorbence at 280 nm, and salt concentration by conductivity, as shown. The activities of column fractions were assayed and plotted ( far left ordinate axis ) and the two activity peaks were pooled. ( B ) Phenyl–Sepharose: pooled fractions from A were applied to a 15-ml phenyl–Sepharose column and proteins eluted with a 300–25 μS salt gradient. ( C ) Q-Sepharose: pooled active fractions from B were diluted to 20 mM salt and chromatographed on a 5-ml Q-Sepharose column. Activity was eluted in a salt gradient to 200 μS. ( D ) 200 μl of the peak fraction from C was applied to a <t>Bio-Silect</t> gel filtration column with a rated separation range of 100–5 kD. The elution positions of molecular weight standards are indicated.
    Se Hplc, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/se hplc/product/Bio-Rad
    Average 91 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    se hplc - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    99
    Bio-Rad gel filtration column
    Purification of the active component from pig brain cytosol. ( A ) Cibachrome blue 3GA: a step-eluted fraction from fast-flow <t>Q-Sepharose,</t> diluted to 50 mM salt, was passed over a 40 ml Cibachrome blue 3GA column, washed extensively, and eluted as shown in a continuous 25-300 μS salt gradient. Protein was monitored in line by absorbence at 280 nm, and salt concentration by conductivity, as shown. The activities of column fractions were assayed and plotted ( far left ordinate axis ) and the two activity peaks were pooled. ( B ) Phenyl–Sepharose: pooled fractions from A were applied to a 15-ml phenyl–Sepharose column and proteins eluted with a 300–25 μS salt gradient. ( C ) Q-Sepharose: pooled active fractions from B were diluted to 20 mM salt and chromatographed on a 5-ml Q-Sepharose column. Activity was eluted in a salt gradient to 200 μS. ( D ) 200 μl of the peak fraction from C was applied to a <t>Bio-Silect</t> gel filtration column with a rated separation range of 100–5 kD. The elution positions of molecular weight standards are indicated.
    Gel Filtration Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gel filtration column/product/Bio-Rad
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gel filtration column - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    85
    Bio-Rad bio silect column
    Purification of the active component from pig brain cytosol. ( A ) Cibachrome blue 3GA: a step-eluted fraction from fast-flow <t>Q-Sepharose,</t> diluted to 50 mM salt, was passed over a 40 ml Cibachrome blue 3GA column, washed extensively, and eluted as shown in a continuous 25-300 μS salt gradient. Protein was monitored in line by absorbence at 280 nm, and salt concentration by conductivity, as shown. The activities of column fractions were assayed and plotted ( far left ordinate axis ) and the two activity peaks were pooled. ( B ) Phenyl–Sepharose: pooled fractions from A were applied to a 15-ml phenyl–Sepharose column and proteins eluted with a 300–25 μS salt gradient. ( C ) Q-Sepharose: pooled active fractions from B were diluted to 20 mM salt and chromatographed on a 5-ml Q-Sepharose column. Activity was eluted in a salt gradient to 200 μS. ( D ) 200 μl of the peak fraction from C was applied to a <t>Bio-Silect</t> gel filtration column with a rated separation range of 100–5 kD. The elution positions of molecular weight standards are indicated.
    Bio Silect Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bio silect column/product/Bio-Rad
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bio silect column - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    Image Search Results


    Purification of the active component from pig brain cytosol. ( A ) Cibachrome blue 3GA: a step-eluted fraction from fast-flow Q-Sepharose, diluted to 50 mM salt, was passed over a 40 ml Cibachrome blue 3GA column, washed extensively, and eluted as shown in a continuous 25-300 μS salt gradient. Protein was monitored in line by absorbence at 280 nm, and salt concentration by conductivity, as shown. The activities of column fractions were assayed and plotted ( far left ordinate axis ) and the two activity peaks were pooled. ( B ) Phenyl–Sepharose: pooled fractions from A were applied to a 15-ml phenyl–Sepharose column and proteins eluted with a 300–25 μS salt gradient. ( C ) Q-Sepharose: pooled active fractions from B were diluted to 20 mM salt and chromatographed on a 5-ml Q-Sepharose column. Activity was eluted in a salt gradient to 200 μS. ( D ) 200 μl of the peak fraction from C was applied to a Bio-Silect gel filtration column with a rated separation range of 100–5 kD. The elution positions of molecular weight standards are indicated.

    Journal: The Journal of Cell Biology

    Article Title: Rho- and Rac-dependent Assembly of Focal Adhesion Complexes and Actin Filaments in Permeabilized Fibroblasts: An Essential Role for Ezrin/Radixin/Moesin Proteins

    doi:

    Figure Lengend Snippet: Purification of the active component from pig brain cytosol. ( A ) Cibachrome blue 3GA: a step-eluted fraction from fast-flow Q-Sepharose, diluted to 50 mM salt, was passed over a 40 ml Cibachrome blue 3GA column, washed extensively, and eluted as shown in a continuous 25-300 μS salt gradient. Protein was monitored in line by absorbence at 280 nm, and salt concentration by conductivity, as shown. The activities of column fractions were assayed and plotted ( far left ordinate axis ) and the two activity peaks were pooled. ( B ) Phenyl–Sepharose: pooled fractions from A were applied to a 15-ml phenyl–Sepharose column and proteins eluted with a 300–25 μS salt gradient. ( C ) Q-Sepharose: pooled active fractions from B were diluted to 20 mM salt and chromatographed on a 5-ml Q-Sepharose column. Activity was eluted in a salt gradient to 200 μS. ( D ) 200 μl of the peak fraction from C was applied to a Bio-Silect gel filtration column with a rated separation range of 100–5 kD. The elution positions of molecular weight standards are indicated.

    Article Snippet: For gel filtration, a 200-μl aliquot of the activity peak from the final Q-Sepharose column was applied to a 6-ml Bio-Silect chromatography column (Bio Rad Laboratories: rated fractionation range 100–5 kD) and protein was eluted in DK150, sampling 0.25-ml fractions.

    Techniques: Purification, Flow Cytometry, Concentration Assay, Activity Assay, Filtration, Molecular Weight