bigdye terminator v3 1 cycle sequencing kit  (Thermo Fisher)


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    Name:
    BigDye Terminator v3 1 Cycle Sequencing Kit
    Description:
    The BigDye Terminator v3 1 Cycle Sequencing Kit s robust highly flexible chemistry is ideal for de novo sequencing resequencing and finishing with PCR product Plasmid Fosmid and BAC templates • Improve the quality of your results for a wide range of sequencing applications • Optimized for long read lengths • Better dye mobility characteristics • Improved performance reading through GT rich regions • Get longer higher quality reads with more uniform peak heights and optimal signal balance • Enhance your productivity and reduce costs Get More Uniform Peak Heights Improved AccuracyWith BigDye Terminator v3 1 kit superior chemistry you generate data with uniform peak heights and optimized signal balance This results in longer higher quality reads and more accurate base assignments for heterozygote and mutation detection For Research Use Only Not for use in diagnostics procedures
    Catalog Number:
    4337454
    Price:
    None
    Applications:
    Kits & Reagents for Sanger Sequencing|Sanger Sequencing|Sanger Sequencing Technology & Accessories|Sequencing
    Category:
    Kits and Assays
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    Structured Review

    Thermo Fisher bigdye terminator v3 1 cycle sequencing kit
    DNA cleavage and strand exchange by SprA. ( A ) Direct sequencing of the intermediates of the SprA-mediated integration reaction. The intermediates: the left and right half-sites of attP , were directly sequenced using a <t>BigDye</t> Terminator <t>v3.1</t> Cycle Sequencing Kit and an ABI 3500 DNA analyzer. The 16 bp overlapping sequence is underlined. The 3΄-end nucleotides indicated by the circles are adenines added by the terminal transferase activity of Taq polymerase used for the cycle sequencing reaction. The cleavage point of attP is shown to the left (indicated by the lines and triangles). ( B ) Effects of point mutations of the central dinucleotides on DNA recombination. Point mutations were introduced into the AAA nucleotides at the center of the attB site (A→T). In vitro integration recombination was performed using 0.5 μM SprA and 50 ng each of the wild-type attP and the mutated attB substrates.
    The BigDye Terminator v3 1 Cycle Sequencing Kit s robust highly flexible chemistry is ideal for de novo sequencing resequencing and finishing with PCR product Plasmid Fosmid and BAC templates • Improve the quality of your results for a wide range of sequencing applications • Optimized for long read lengths • Better dye mobility characteristics • Improved performance reading through GT rich regions • Get longer higher quality reads with more uniform peak heights and optimal signal balance • Enhance your productivity and reduce costs Get More Uniform Peak Heights Improved AccuracyWith BigDye Terminator v3 1 kit superior chemistry you generate data with uniform peak heights and optimized signal balance This results in longer higher quality reads and more accurate base assignments for heterozygote and mutation detection For Research Use Only Not for use in diagnostics procedures
    https://www.bioz.com/result/bigdye terminator v3 1 cycle sequencing kit/product/Thermo Fisher
    Average 99 stars, based on 5249 article reviews
    Price from $9.99 to $1999.99
    bigdye terminator v3 1 cycle sequencing kit - by Bioz Stars, 2020-07
    99/100 stars

    Images

    1) Product Images from "Mechanism of bacterial gene rearrangement: SprA-catalyzed precise DNA recombination and its directionality control by SprB ensure the gene rearrangement and stable expression of spsM during sporulation in Bacillus subtilis"

    Article Title: Mechanism of bacterial gene rearrangement: SprA-catalyzed precise DNA recombination and its directionality control by SprB ensure the gene rearrangement and stable expression of spsM during sporulation in Bacillus subtilis

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkx466

    DNA cleavage and strand exchange by SprA. ( A ) Direct sequencing of the intermediates of the SprA-mediated integration reaction. The intermediates: the left and right half-sites of attP , were directly sequenced using a BigDye Terminator v3.1 Cycle Sequencing Kit and an ABI 3500 DNA analyzer. The 16 bp overlapping sequence is underlined. The 3΄-end nucleotides indicated by the circles are adenines added by the terminal transferase activity of Taq polymerase used for the cycle sequencing reaction. The cleavage point of attP is shown to the left (indicated by the lines and triangles). ( B ) Effects of point mutations of the central dinucleotides on DNA recombination. Point mutations were introduced into the AAA nucleotides at the center of the attB site (A→T). In vitro integration recombination was performed using 0.5 μM SprA and 50 ng each of the wild-type attP and the mutated attB substrates.
    Figure Legend Snippet: DNA cleavage and strand exchange by SprA. ( A ) Direct sequencing of the intermediates of the SprA-mediated integration reaction. The intermediates: the left and right half-sites of attP , were directly sequenced using a BigDye Terminator v3.1 Cycle Sequencing Kit and an ABI 3500 DNA analyzer. The 16 bp overlapping sequence is underlined. The 3΄-end nucleotides indicated by the circles are adenines added by the terminal transferase activity of Taq polymerase used for the cycle sequencing reaction. The cleavage point of attP is shown to the left (indicated by the lines and triangles). ( B ) Effects of point mutations of the central dinucleotides on DNA recombination. Point mutations were introduced into the AAA nucleotides at the center of the attB site (A→T). In vitro integration recombination was performed using 0.5 μM SprA and 50 ng each of the wild-type attP and the mutated attB substrates.

    Techniques Used: Sequencing, Activity Assay, In Vitro

    2) Product Images from "Familial STAG2 germline mutation defines a new human cohesinopathy"

    Article Title: Familial STAG2 germline mutation defines a new human cohesinopathy

    Journal: NPJ Genomic Medicine

    doi: 10.1038/s41525-017-0009-4

    Pedigree. a Pedigree shows typical X-linked recessive inheritance of the c.980 G > A (p.Ser327Asn) in the STAG2 gene (NM_001042749.1) on the X chromosome. The variant was studied in the proband ( arrow ) and in male and female relatives by allele-specific PCR and confirmed in the proband and all affected male relatives by Sanger sequencing. b For Sanger sequencing we used the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems ® ) and the Applied Biosystems (ABI) 3130 Genetic Analyzer. Sequencing data were analyzed using the software Sequencher version 4.1.4 (Gene Code Corporation)
    Figure Legend Snippet: Pedigree. a Pedigree shows typical X-linked recessive inheritance of the c.980 G > A (p.Ser327Asn) in the STAG2 gene (NM_001042749.1) on the X chromosome. The variant was studied in the proband ( arrow ) and in male and female relatives by allele-specific PCR and confirmed in the proband and all affected male relatives by Sanger sequencing. b For Sanger sequencing we used the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems ® ) and the Applied Biosystems (ABI) 3130 Genetic Analyzer. Sequencing data were analyzed using the software Sequencher version 4.1.4 (Gene Code Corporation)

    Techniques Used: Variant Assay, Polymerase Chain Reaction, Sequencing, Software

    Related Articles

    Amplification:

    Article Title: BAP1 Missense Mutation c.2054 A > T (p.E685V) Completely Disrupts Normal Splicing through Creation of a Novel 5’ Splice Site in a Human Mesothelioma Cell Line
    Article Snippet: .. DNA from colonies was amplified using the BAP1 primers covering cDNA regions of exons 14–17 and subjected to direct DNA sequencing analysis using the forward PCR primer (BigDye Terminator v3.1 Cycle Sequencing kit and 3730 DNA analyzer, Applied Biosystems, Foster City, CA). .. Results Multi-species comparative genomic analysis was used to identify sequence homology at the BAP1 c .2054 A>T (p.E685V) mutation site in ten distantly related species: human, chimpanzee, mouse, rat, dog, cat, rabbit, chicken, xenopus and zebrafish.

    Purification:

    Article Title: Mechanism of bacterial gene rearrangement: SprA-catalyzed precise DNA recombination and its directionality control by SprB ensure the gene rearrangement and stable expression of spsM during sporulation in Bacillus subtilis
    Article Snippet: .. DNA pellets were dissolved in TE buffer, gel purified, and directly sequenced using the P107 (for the left half- site of attP ) or P109 (for the right half- site) primers, a BigDye® Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific, WI, USA) and an ABI 3500 DNA analyzer (Thermo Fisher Scientific). .. Electrophoresis mobility shift assays DNA fragments containing the att sites were amplified with the primer sets P110/P61 (for attP ; 89 bp), P111/P87 (for attB ; 106 bp), P111/P58 (for attL ; 111 bp), and P7/P87 (for attR ; 132 bp).

    Electrophoresis:

    Article Title: Whole-exome sequencing identifies novel candidate predisposition genes for familial polycythemia vera
    Article Snippet: .. BigDye Terminator v3.1 sequencing reaction was used in the DNA sequencing, and capillary electrophoresis was performed on an ABI3730xl DNA Analyzer (Applied Biosystems, Foster City, CA, USA) at the Institute for Molecular Medicine Finland (FIMM). .. The results were analyzed manually using FinchTV v.1.4.0 (Geospiza Inc., Seattle, WA, USA).

    Sequencing:

    Article Title: Familial STAG2 germline mutation defines a new human cohesinopathy
    Article Snippet: .. Sanger sequencing was achieved by standard methods using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems® ) and the Applied Biosystems (ABI) 3130 Genetic Analyzer. .. Sequencing data was analyzed using the software Sequencher version 4.1.4 (Gene Code).

    Article Title: Whole-exome sequencing identifies novel candidate predisposition genes for familial polycythemia vera
    Article Snippet: .. BigDye Terminator v3.1 sequencing reaction was used in the DNA sequencing, and capillary electrophoresis was performed on an ABI3730xl DNA Analyzer (Applied Biosystems, Foster City, CA, USA) at the Institute for Molecular Medicine Finland (FIMM). .. The results were analyzed manually using FinchTV v.1.4.0 (Geospiza Inc., Seattle, WA, USA).

    Article Title: BAP1 Missense Mutation c.2054 A > T (p.E685V) Completely Disrupts Normal Splicing through Creation of a Novel 5’ Splice Site in a Human Mesothelioma Cell Line
    Article Snippet: .. DNA from colonies was amplified using the BAP1 primers covering cDNA regions of exons 14–17 and subjected to direct DNA sequencing analysis using the forward PCR primer (BigDye Terminator v3.1 Cycle Sequencing kit and 3730 DNA analyzer, Applied Biosystems, Foster City, CA). .. Results Multi-species comparative genomic analysis was used to identify sequence homology at the BAP1 c .2054 A>T (p.E685V) mutation site in ten distantly related species: human, chimpanzee, mouse, rat, dog, cat, rabbit, chicken, xenopus and zebrafish.

    Article Title: Characterization of the biology and infectivity of Leishmania infantum viscerotropic and dermotropic strains isolated from HIV+ and HIV- patients in the murine model of visceral leishmaniasis
    Article Snippet: .. The Big-Dye Terminator Cycle Sequencing Ready Reaction Kit V3.1 and the automated ABI PRISM 377 DNA sequencer (Applied Biosystems) were used for direct sequencing of the k26 , ITS1 and ITS2 PCR products that was performed with the corresponding forward and reverse primers; internal primers for sequencing were also used for the hsp70 PCR product, as described by Fraga et al . .. The obtained sequences were analyzed and edited using the software BioEdit Sequence Alignment Editor, version 7.0.9.0 (Ibis Biosciences) [ ].

    Article Title: Mechanism of bacterial gene rearrangement: SprA-catalyzed precise DNA recombination and its directionality control by SprB ensure the gene rearrangement and stable expression of spsM during sporulation in Bacillus subtilis
    Article Snippet: .. DNA pellets were dissolved in TE buffer, gel purified, and directly sequenced using the P107 (for the left half- site of attP ) or P109 (for the right half- site) primers, a BigDye® Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific, WI, USA) and an ABI 3500 DNA analyzer (Thermo Fisher Scientific). .. Electrophoresis mobility shift assays DNA fragments containing the att sites were amplified with the primer sets P110/P61 (for attP ; 89 bp), P111/P87 (for attB ; 106 bp), P111/P58 (for attL ; 111 bp), and P7/P87 (for attR ; 132 bp).

    Article Title: p-Hydroxybenzoyl-Glucose Is a Zwitter Donor for the Biosynthesis of 7-Polyacylated Anthocyanin in Delphinium [W]
    Article Snippet: .. The sequences of the cDNA fragments were determined using a BigDye-Terminator ver.3.1 cycle sequencing kit and an ABI PRISM 3130xl (Applied Biosystems). .. The 5′ and 3′ cDNA ends of SCPLs and AA7BG-GTs were amplified with a SMARTer RACE cDNA amplification kit (Clontech Laboratories).

    Article Title: DNA sequence analysis suggests that cytb-nd1 PCR-RFLP may not be applicable to sandfly species identification throughout the Mediterranean region
    Article Snippet: .. The Big-Dye Terminator Cycle Sequencing Ready Reaction Kit V3.1 and the automated ABI PRISM 377 DNA sequencer (Applied Biosystems, Foster City, CA) were used. .. Sequences obtained were analyzed and edited using the software Molecular Evolutionary Genetics Analysis (MEGA) version 5.2 (Tamura et al. ).

    DNA Sequencing:

    Article Title: Whole-exome sequencing identifies novel candidate predisposition genes for familial polycythemia vera
    Article Snippet: .. BigDye Terminator v3.1 sequencing reaction was used in the DNA sequencing, and capillary electrophoresis was performed on an ABI3730xl DNA Analyzer (Applied Biosystems, Foster City, CA, USA) at the Institute for Molecular Medicine Finland (FIMM). .. The results were analyzed manually using FinchTV v.1.4.0 (Geospiza Inc., Seattle, WA, USA).

    Article Title: BAP1 Missense Mutation c.2054 A > T (p.E685V) Completely Disrupts Normal Splicing through Creation of a Novel 5’ Splice Site in a Human Mesothelioma Cell Line
    Article Snippet: .. DNA from colonies was amplified using the BAP1 primers covering cDNA regions of exons 14–17 and subjected to direct DNA sequencing analysis using the forward PCR primer (BigDye Terminator v3.1 Cycle Sequencing kit and 3730 DNA analyzer, Applied Biosystems, Foster City, CA). .. Results Multi-species comparative genomic analysis was used to identify sequence homology at the BAP1 c .2054 A>T (p.E685V) mutation site in ten distantly related species: human, chimpanzee, mouse, rat, dog, cat, rabbit, chicken, xenopus and zebrafish.

    Polymerase Chain Reaction:

    Article Title: BAP1 Missense Mutation c.2054 A > T (p.E685V) Completely Disrupts Normal Splicing through Creation of a Novel 5’ Splice Site in a Human Mesothelioma Cell Line
    Article Snippet: .. DNA from colonies was amplified using the BAP1 primers covering cDNA regions of exons 14–17 and subjected to direct DNA sequencing analysis using the forward PCR primer (BigDye Terminator v3.1 Cycle Sequencing kit and 3730 DNA analyzer, Applied Biosystems, Foster City, CA). .. Results Multi-species comparative genomic analysis was used to identify sequence homology at the BAP1 c .2054 A>T (p.E685V) mutation site in ten distantly related species: human, chimpanzee, mouse, rat, dog, cat, rabbit, chicken, xenopus and zebrafish.

    Article Title: Characterization of the biology and infectivity of Leishmania infantum viscerotropic and dermotropic strains isolated from HIV+ and HIV- patients in the murine model of visceral leishmaniasis
    Article Snippet: .. The Big-Dye Terminator Cycle Sequencing Ready Reaction Kit V3.1 and the automated ABI PRISM 377 DNA sequencer (Applied Biosystems) were used for direct sequencing of the k26 , ITS1 and ITS2 PCR products that was performed with the corresponding forward and reverse primers; internal primers for sequencing were also used for the hsp70 PCR product, as described by Fraga et al . .. The obtained sequences were analyzed and edited using the software BioEdit Sequence Alignment Editor, version 7.0.9.0 (Ibis Biosciences) [ ].

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    Thermo Fisher bigdye terminater v3 1 cycle sequencing kit
    Bigdye Terminater V3 1 Cycle Sequencing Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bigdye terminater v3 1 cycle sequencing kit/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bigdye terminater v3 1 cycle sequencing kit - by Bioz Stars, 2020-07
    99/100 stars
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