bigdye terminator v3 1 cycle sequencing kit  (Thermo Fisher)


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    Thermo Fisher bigdye terminator v3 1 cycle sequencing kit
    Bigdye Terminator V3 1 Cycle Sequencing Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3052 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bigdye terminator v3 1 cycle sequencing kit/product/Thermo Fisher
    Average 99 stars, based on 3052 article reviews
    Price from $9.99 to $1999.99
    bigdye terminator v3 1 cycle sequencing kit - by Bioz Stars, 2020-01
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Genotoxin Induced Mutagenesis in the Model Plant Physcomitrella patens
    Article Snippet: Some clones were further propagated on plates with 8 mM 2-FA and their APT locus was subsequently PCR amplified and sequenced to identify the mutation(s) responsible for the resistance. .. 28104) and used as a template for sequencing with BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, cat. Nr.

    Article Title: Design of N-Terminal Derivatives from a Novel Dermaseptin Exhibiting Broad-Spectrum Antimicrobial Activity against Isolates from Cystic Fibrosis Patients
    Article Snippet: The cDNA was amplified via RACE techniques and the purified products were cloned by pGEM-T easy vector system (Promega, Madison, W1, USA). .. Finally, each clone was sequenced by ABI 3100 automated capillary sequencer (Applied Biosystems, Foster City, CA, USA) using BigDye Terminator v3.1 Cycle Sequencing Kit (Life Technologies, Paisley, UK).

    Article Title: Histology and molecular biology studies on the expression and localization of angiopoietin-like protein 8 in human tissues
    Article Snippet: .. Cloned ANGPTL8 fragments were sequenced using the BigDye Terminator v3.1 Cycle Sequencing kit (Thermo Fisher Scientific, Inc.). .. FFPE specimens of lipoma, hibernoma and normal human tissues of the liver, adipose tissue, heart, stomach, small intestine, large intestine, pancreas, spleen, kidney, lymph node, bone marrow, thyroid, adrenal gland, ovary and testis were used in the present study.

    Amplification:

    Article Title: Genotoxin Induced Mutagenesis in the Model Plant Physcomitrella patens
    Article Snippet: APT locus was amplified from isolated genomic DNA with KOD Hot Start DNA Polymerase (Millipore/Novagen, cat. Nr. .. 28104) and used as a template for sequencing with BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, cat. Nr.

    Article Title: Rapidly declining trend of signet ring cell cancer of the stomach may parallel the infection rate of Helicobacter pylori
    Article Snippet: Reactions were subject to thermal cycling with the following conditions: 94 °C for 2 min, 45 cycles of 94 °C for 15 s, 60 °C for 15 s, and 68 °C for 30 s. To determine the H. pylori- specific amplicon, 5 μL of the PCR products was separated by electrophoresis on 2% agarose gel, stained with ethidium bromide and visualized on an FAS IV UV Illuminator (NIPPON Genetics, Japan) [ , ]. .. Sanger sequencing was performed with BigDye Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific) using forward or reverse primers.

    Article Title: A cardiac and subcutaneous canine dirofilariosis outbreak in a kennel in central France
    Article Snippet: .. The DNA generated through the PCR reaction was purified by filtration using a NucleoFast® 96 PCR DNA purification plate, and was then amplified using a BigDye® Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA). .. The BigDye PCR products were purified on the Sephadex G-50 Superfine gel filtration resin prior to sequencing on the ABI Prism 3130XL.

    Article Title: Highly Predictive Genetic Markers Distinguish Drug-Type from Fiber-Type Cannabis sativa L
    Article Snippet: After being stained with ethidium bromide, the amplified products were photographed under UV light (254 nm). .. Sequencing was conducted using a BigDye Terminator v3.1 Cycle Sequencing Kit (Life Technologies, Carlsbad, CA, USA) as follows: 4 μL reaction mix, 3.2 pmol of primer, and 2 μL of the purified PCR product in 15 μL total volume.

    Article Title: Natural Human Infections With Plasmodium cynomolgi and Other Malaria Species in an Elimination Setting in Sabah, Malaysia
    Article Snippet: .. The amplified 18S rRNA gene was subsequently sequenced on the ABI 3730xl DNA analyzer, using the BigDye terminator v3.1 Cycle Sequencing Kit (Applied BioSystems) and primers from nest 2 of the 18s rRNA PCR. ..

    Article Title: Accurate detection of KRAS, NRAS and BRAF mutations in metastatic colorectal cancers by bridged nucleic acid-clamp real-time PCR
    Article Snippet: Sanger sequencing To further determine nucleotide changes and to characterize the deduced amino acid changes, we performed Sanger sequencing on samples, in which PCR amplification plot was observed but did not reached to threshold line by BNA-clamp PCR. .. Purified products were used as templates and Sanger sequencing was performed using the BNA Real-time PCR Extended RAS Mutation Sequencing Primer (Riken Genesis) and the BigDye® Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific).

    Article Title: Salmonella in Retail Food and Wild Birds in Singapore—Prevalence, Antimicrobial Resistance, and Sequence Types
    Article Snippet: .. The amplified fragments were visualised on 2% agarose gels and subsequently; purified and sequenced using BigDye® Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Waltham, MA, USA). .. Raw sequences were then assembled in Lasergene Software version 8.0 (DNASTAR, Madison, WI, USA) and the consensus sequences were compared with those available in the MLST database ( http://enterobase.warwick.ac.uk/species/senterica/allele_st_search ) to determine allelic numbers and sequence types.

    Article Title: Known and novel mutations responsible for epidermolysis bullosa simplex cases in a Chinese population
    Article Snippet: Genomic DNA samples were amplified by PCR using Takara Ex Taq DNA polymerase (Takara Bio, Inc.). .. The Sequencing Reaction system was based on BigDye® Terminator v3.1 Cycle Sequencing kit (Thermo Fisher Scientific, Inc.) according to the manufacturer's instructions.

    Article Title: Impact of Preexisting Hepatitis C Virus Genotype 6 NS3, NS5A, and NS5B Polymorphisms on the In Vitro Potency of Direct-Acting Antiviral Agents
    Article Snippet: .. The NS3, NS5A, and NS5B coding regions were amplified, population-based sequencing (threshold of ≥15% for reporting polymorphisms at each residue position) was performed using the BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems, Foster City, CA), and genotype-specific primers were selected from the list in Table S1 in the supplemental material. .. A GT-6a consensus sequence was generated for each target gene from each sample.

    Article Title: Design of N-Terminal Derivatives from a Novel Dermaseptin Exhibiting Broad-Spectrum Antimicrobial Activity against Isolates from Cystic Fibrosis Patients
    Article Snippet: The cDNA was amplified via RACE techniques and the purified products were cloned by pGEM-T easy vector system (Promega, Madison, W1, USA). .. Finally, each clone was sequenced by ABI 3100 automated capillary sequencer (Applied Biosystems, Foster City, CA, USA) using BigDye Terminator v3.1 Cycle Sequencing Kit (Life Technologies, Paisley, UK).

    Article Title: Histology and molecular biology studies on the expression and localization of angiopoietin-like protein 8 in human tissues
    Article Snippet: The amplified PCR product was cloned into pCR-II TOPO (Thermo Fisher Scientific, Inc.) using the TA-cloning method. .. Cloned ANGPTL8 fragments were sequenced using the BigDye Terminator v3.1 Cycle Sequencing kit (Thermo Fisher Scientific, Inc.).

    Article Title: Investigation on the hereditary basis of colorectal cancers in an African population with frequent early onset cases
    Article Snippet: Sequencing was performed using BigDye terminator v3.1 cycle sequencing kit (Thermofisher Scientific, Wilmington, USA) following standard protocols. .. Briefly, germline DNA was denatured, followed by hybridisation with MLPA probes, ligation and amplification.

    Filtration:

    Article Title: A cardiac and subcutaneous canine dirofilariosis outbreak in a kennel in central France
    Article Snippet: .. The DNA generated through the PCR reaction was purified by filtration using a NucleoFast® 96 PCR DNA purification plate, and was then amplified using a BigDye® Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA). .. The BigDye PCR products were purified on the Sephadex G-50 Superfine gel filtration resin prior to sequencing on the ABI Prism 3130XL.

    Synthesized:

    Article Title: Histology and molecular biology studies on the expression and localization of angiopoietin-like protein 8 in human tissues
    Article Snippet: Total RNA (2 µg) was treated with DNase I (Thermo Fisher Scientific, Inc.) at room temperature (RT) for 15 min, and cDNA was synthesized by random primer method using the SuperScript III First Strand Synthesis kit (Thermo Fisher Scientific, Inc.) according to the manufacturer's instructions. .. Cloned ANGPTL8 fragments were sequenced using the BigDye Terminator v3.1 Cycle Sequencing kit (Thermo Fisher Scientific, Inc.).

    TA Cloning:

    Article Title: Histology and molecular biology studies on the expression and localization of angiopoietin-like protein 8 in human tissues
    Article Snippet: The amplified PCR product was cloned into pCR-II TOPO (Thermo Fisher Scientific, Inc.) using the TA-cloning method. .. Cloned ANGPTL8 fragments were sequenced using the BigDye Terminator v3.1 Cycle Sequencing kit (Thermo Fisher Scientific, Inc.).

    Construct:

    Article Title: Molecular Epidemiological Characterization of Staphylococcus argenteus Clinical Isolates in Japan: Identification of Three Clones (ST1223, ST2198, and ST2550) and a Novel Staphylocoagulase Genotype XV
    Article Snippet: Phylogenetic Analysis of Virulence Factors Nucleotide sequences of full-length ORF were determined for staphylocoagulase genes belonging to a novel type, microbial surface component recognizing adhesive matrix molecule (MSCRAMM) family adhesin SdrE gene (sdrE ), elastin-binding protein gene (ebpS ), SE(-like) genes (selw , selx , sey , selz , sel26 , sel27 ), and staphylokinase gene (sak ) by PCR with primers listed in , followed by Sanger sequencing using the BigDye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) on an automated DNA sequencer (ABI PRISM 3100). .. Phylogenetic dendrograms of these virulence factors were constructed by the maximum likelihood method using the MEGA7 software, together with sequence data of staphylococcal strains available in GenBank database.

    Article Title: Design of N-Terminal Derivatives from a Novel Dermaseptin Exhibiting Broad-Spectrum Antimicrobial Activity against Isolates from Cystic Fibrosis Patients
    Article Snippet: Identification of DM-PC Precursor-Encoding cDNA from Skin Secretion The mRNA from skin secretion was isolated and skin secretion-derived cDNA library of Phyllomedusa coelestis was constructed as described previously [ ]. .. Finally, each clone was sequenced by ABI 3100 automated capillary sequencer (Applied Biosystems, Foster City, CA, USA) using BigDye Terminator v3.1 Cycle Sequencing Kit (Life Technologies, Paisley, UK).

    Electrophoresis:

    Article Title: Rapidly declining trend of signet ring cell cancer of the stomach may parallel the infection rate of Helicobacter pylori
    Article Snippet: Reactions were subject to thermal cycling with the following conditions: 94 °C for 2 min, 45 cycles of 94 °C for 15 s, 60 °C for 15 s, and 68 °C for 30 s. To determine the H. pylori- specific amplicon, 5 μL of the PCR products was separated by electrophoresis on 2% agarose gel, stained with ethidium bromide and visualized on an FAS IV UV Illuminator (NIPPON Genetics, Japan) [ , ]. .. Sanger sequencing was performed with BigDye Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific) using forward or reverse primers.

    Article Title: Investigation on the hereditary basis of colorectal cancers in an African population with frequent early onset cases
    Article Snippet: Sequencing was performed using BigDye terminator v3.1 cycle sequencing kit (Thermofisher Scientific, Wilmington, USA) following standard protocols. .. The amplified products were visualised using capillary electrophoresis, and the output analysed using the Coffalyser.Net software ( https://www.mlpa.com ).

    Incubation:

    Article Title: A cardiac and subcutaneous canine dirofilariosis outbreak in a kennel in central France
    Article Snippet: The thermal cycling conditions were: incubation step at 95 °C for 15 min, 40 cycles of 1 min at 95 °C, 30 s at 54 °C for the melting temperature, and one and half minutes for the elongation time at 72 °C, followed by a final extension of 5 min at 72 °C. .. The DNA generated through the PCR reaction was purified by filtration using a NucleoFast® 96 PCR DNA purification plate, and was then amplified using a BigDye® Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA).

    Hybridization:

    Article Title: Investigation on the hereditary basis of colorectal cancers in an African population with frequent early onset cases
    Article Snippet: Sequencing was performed using BigDye terminator v3.1 cycle sequencing kit (Thermofisher Scientific, Wilmington, USA) following standard protocols. .. Briefly, germline DNA was denatured, followed by hybridisation with MLPA probes, ligation and amplification.

    Ligation:

    Article Title: Investigation on the hereditary basis of colorectal cancers in an African population with frequent early onset cases
    Article Snippet: Sequencing was performed using BigDye terminator v3.1 cycle sequencing kit (Thermofisher Scientific, Wilmington, USA) following standard protocols. .. Briefly, germline DNA was denatured, followed by hybridisation with MLPA probes, ligation and amplification.

    Generated:

    Article Title: A cardiac and subcutaneous canine dirofilariosis outbreak in a kennel in central France
    Article Snippet: .. The DNA generated through the PCR reaction was purified by filtration using a NucleoFast® 96 PCR DNA purification plate, and was then amplified using a BigDye® Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA). .. The BigDye PCR products were purified on the Sephadex G-50 Superfine gel filtration resin prior to sequencing on the ABI Prism 3130XL.

    Article Title: Impact of Preexisting Hepatitis C Virus Genotype 6 NS3, NS5A, and NS5B Polymorphisms on the In Vitro Potency of Direct-Acting Antiviral Agents
    Article Snippet: The NS3, NS5A, and NS5B coding regions were amplified, population-based sequencing (threshold of ≥15% for reporting polymorphisms at each residue position) was performed using the BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems, Foster City, CA), and genotype-specific primers were selected from the list in Table S1 in the supplemental material. .. A GT-6a consensus sequence was generated for each target gene from each sample.

    Sequencing:

    Article Title: Genotoxin Induced Mutagenesis in the Model Plant Physcomitrella patens
    Article Snippet: .. 28104) and used as a template for sequencing with BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, cat. Nr. .. Locations of PCR primers used for APT amplification and sequencing are depicted on Supplementary Figure 2 and their sequences are listed in Supplementary Table 1 (see Supplementary Material available online at http://dx.doi.org/10.1155/2013/535049 ).

    Article Title: Molecular Epidemiological Characterization of Staphylococcus argenteus Clinical Isolates in Japan: Identification of Three Clones (ST1223, ST2198, and ST2550) and a Novel Staphylocoagulase Genotype XV
    Article Snippet: .. Phylogenetic Analysis of Virulence Factors Nucleotide sequences of full-length ORF were determined for staphylocoagulase genes belonging to a novel type, microbial surface component recognizing adhesive matrix molecule (MSCRAMM) family adhesin SdrE gene (sdrE ), elastin-binding protein gene (ebpS ), SE(-like) genes (selw , selx , sey , selz , sel26 , sel27 ), and staphylokinase gene (sak ) by PCR with primers listed in , followed by Sanger sequencing using the BigDye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) on an automated DNA sequencer (ABI PRISM 3100). .. Similarly, full length sequences of enterotoxin gene sec and tst-1 gene were also determined.

    Article Title: Rapidly declining trend of signet ring cell cancer of the stomach may parallel the infection rate of Helicobacter pylori
    Article Snippet: .. Sanger sequencing was performed with BigDye Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific) using forward or reverse primers. .. PCR products were purified BigDye XTerminator™ Purification Kit and subsequently analyzed by 3500 Genetic Analyzer (Thermo Fisher Scientific).

    Article Title: A single-nucleotide polymorphism influences brain morphology in drug-naïve patients with major depressive disorder
    Article Snippet: .. Genotyping was carried out with a PCR SNP genotyping system using the BigDye Terminator v3.1 Cycle Sequencing Kit (Life Technologies Corporation, Tokyo, Japan). .. The DNA was read using a BMG Applied Biosystem 3730xI DNA Analyzer (Life Technologies Corporation).

    Article Title: A cardiac and subcutaneous canine dirofilariosis outbreak in a kennel in central France
    Article Snippet: .. The DNA generated through the PCR reaction was purified by filtration using a NucleoFast® 96 PCR DNA purification plate, and was then amplified using a BigDye® Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA). .. The BigDye PCR products were purified on the Sephadex G-50 Superfine gel filtration resin prior to sequencing on the ABI Prism 3130XL.

    Article Title: Inherited Variants in BLM and the Risk and Clinical Characteristics of Breast Cancer
    Article Snippet: .. Sequencing reactions were performed using a BigDye Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol. .. Sequencing products were analyzed on the ABI prism 3100 Genetic Analyzer (Thermo Fisher Scientific).

    Article Title: Highly Predictive Genetic Markers Distinguish Drug-Type from Fiber-Type Cannabis sativa L
    Article Snippet: .. Sequencing was conducted using a BigDye Terminator v3.1 Cycle Sequencing Kit (Life Technologies, Carlsbad, CA, USA) as follows: 4 μL reaction mix, 3.2 pmol of primer, and 2 μL of the purified PCR product in 15 μL total volume. .. The sequences were purified with a BigDye XTerminator Purification Kit and analyzed with an ABI PRISM 3130 Genetic Analyzer (Applied Biosystems).

    Article Title: Natural Human Infections With Plasmodium cynomolgi and Other Malaria Species in an Elimination Setting in Sabah, Malaysia
    Article Snippet: .. The amplified 18S rRNA gene was subsequently sequenced on the ABI 3730xl DNA analyzer, using the BigDye terminator v3.1 Cycle Sequencing Kit (Applied BioSystems) and primers from nest 2 of the 18s rRNA PCR. ..

    Article Title: Accurate detection of KRAS, NRAS and BRAF mutations in metastatic colorectal cancers by bridged nucleic acid-clamp real-time PCR
    Article Snippet: .. Purified products were used as templates and Sanger sequencing was performed using the BNA Real-time PCR Extended RAS Mutation Sequencing Primer (Riken Genesis) and the BigDye® Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific). .. Sequencing reactions consisted of 1.0 μL template PCR product, 0.5 μL 3.2 μM forward primer or 0.5 μL 3.2 μM reverse primer, 2 μL Big Dye Buffer, 1 μL BigDye v3.1 and 5.5 μL nuclease free water.

    Article Title: Known and novel mutations responsible for epidermolysis bullosa simplex cases in a Chinese population
    Article Snippet: .. The Sequencing Reaction system was based on BigDye® Terminator v3.1 Cycle Sequencing kit (Thermo Fisher Scientific, Inc.) according to the manufacturer's instructions. .. Purified PCR products were directly sequenced using an ABI PRISM® 3730 automated sequencer (Applied Biosystems; Thermo Fisher Scientific, Inc.).

    Article Title: Impact of Preexisting Hepatitis C Virus Genotype 6 NS3, NS5A, and NS5B Polymorphisms on the In Vitro Potency of Direct-Acting Antiviral Agents
    Article Snippet: .. The NS3, NS5A, and NS5B coding regions were amplified, population-based sequencing (threshold of ≥15% for reporting polymorphisms at each residue position) was performed using the BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems, Foster City, CA), and genotype-specific primers were selected from the list in Table S1 in the supplemental material. .. A GT-6a consensus sequence was generated for each target gene from each sample.

    Article Title: Design of N-Terminal Derivatives from a Novel Dermaseptin Exhibiting Broad-Spectrum Antimicrobial Activity against Isolates from Cystic Fibrosis Patients
    Article Snippet: .. Finally, each clone was sequenced by ABI 3100 automated capillary sequencer (Applied Biosystems, Foster City, CA, USA) using BigDye Terminator v3.1 Cycle Sequencing Kit (Life Technologies, Paisley, UK). .. Isolation of the Putative Mature Peptide from Skin Secretion The isolation and identification of mature peptides in skin secretion were performed as in a previous study [ ].

    Article Title: Histology and molecular biology studies on the expression and localization of angiopoietin-like protein 8 in human tissues
    Article Snippet: .. Cloned ANGPTL8 fragments were sequenced using the BigDye Terminator v3.1 Cycle Sequencing kit (Thermo Fisher Scientific, Inc.). .. FFPE specimens of lipoma, hibernoma and normal human tissues of the liver, adipose tissue, heart, stomach, small intestine, large intestine, pancreas, spleen, kidney, lymph node, bone marrow, thyroid, adrenal gland, ovary and testis were used in the present study.

    TaqMan Assay:

    Article Title: Inherited Variants in BLM and the Risk and Clinical Characteristics of Breast Cancer
    Article Snippet: The c.1642C > T (p.Gln548Ter) mutation was genotyped using a TaqMan assay (Thermo Fisher Scientific, Waltham, MA, USA) in a LightCycler Real-Time PCR 480 System (Roche Life Science, Mannheim, Germany). .. Sequencing reactions were performed using a BigDye Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol.

    Mutagenesis:

    Article Title: Genotoxin Induced Mutagenesis in the Model Plant Physcomitrella patens
    Article Snippet: Some clones were further propagated on plates with 8 mM 2-FA and their APT locus was subsequently PCR amplified and sequenced to identify the mutation(s) responsible for the resistance. .. 28104) and used as a template for sequencing with BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, cat. Nr.

    Article Title: Inherited Variants in BLM and the Risk and Clinical Characteristics of Breast Cancer
    Article Snippet: The c.1642C > T (p.Gln548Ter) mutation was genotyped using a TaqMan assay (Thermo Fisher Scientific, Waltham, MA, USA) in a LightCycler Real-Time PCR 480 System (Roche Life Science, Mannheim, Germany). .. Sequencing reactions were performed using a BigDye Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol.

    Article Title: Accurate detection of KRAS, NRAS and BRAF mutations in metastatic colorectal cancers by bridged nucleic acid-clamp real-time PCR
    Article Snippet: .. Purified products were used as templates and Sanger sequencing was performed using the BNA Real-time PCR Extended RAS Mutation Sequencing Primer (Riken Genesis) and the BigDye® Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific). .. Sequencing reactions consisted of 1.0 μL template PCR product, 0.5 μL 3.2 μM forward primer or 0.5 μL 3.2 μM reverse primer, 2 μL Big Dye Buffer, 1 μL BigDye v3.1 and 5.5 μL nuclease free water.

    Article Title: Investigation on the hereditary basis of colorectal cancers in an African population with frequent early onset cases
    Article Snippet: All cases that were MLH1 protein deficient, with a high level of microsatellite instability (MSI-H) were sequenced for the BRAF V600E mutation. .. Sequencing was performed using BigDye terminator v3.1 cycle sequencing kit (Thermofisher Scientific, Wilmington, USA) following standard protocols.

    Isolation:

    Article Title: Genotoxin Induced Mutagenesis in the Model Plant Physcomitrella patens
    Article Snippet: Paragraph title: 2.5. Isolation and Analysis of apt Mutants after Bleomycin Treatment ... 28104) and used as a template for sequencing with BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, cat. Nr.

    Article Title: A single-nucleotide polymorphism influences brain morphology in drug-naïve patients with major depressive disorder
    Article Snippet: DNA was isolated from peripheral blood mononuclear cells using the QIAamp DNA Mini-Kit (QIAGEN, Tokyo, Japan). .. Genotyping was carried out with a PCR SNP genotyping system using the BigDye Terminator v3.1 Cycle Sequencing Kit (Life Technologies Corporation, Tokyo, Japan).

    Article Title: Highly Predictive Genetic Markers Distinguish Drug-Type from Fiber-Type Cannabis sativa L
    Article Snippet: Paragraph title: 2.3. Isolation and Sequencing of DNA ... Sequencing was conducted using a BigDye Terminator v3.1 Cycle Sequencing Kit (Life Technologies, Carlsbad, CA, USA) as follows: 4 μL reaction mix, 3.2 pmol of primer, and 2 μL of the purified PCR product in 15 μL total volume.

    Article Title: Design of N-Terminal Derivatives from a Novel Dermaseptin Exhibiting Broad-Spectrum Antimicrobial Activity against Isolates from Cystic Fibrosis Patients
    Article Snippet: Identification of DM-PC Precursor-Encoding cDNA from Skin Secretion The mRNA from skin secretion was isolated and skin secretion-derived cDNA library of Phyllomedusa coelestis was constructed as described previously [ ]. .. Finally, each clone was sequenced by ABI 3100 automated capillary sequencer (Applied Biosystems, Foster City, CA, USA) using BigDye Terminator v3.1 Cycle Sequencing Kit (Life Technologies, Paisley, UK).

    Size-exclusion Chromatography:

    Article Title: Molecular Epidemiological Characterization of Staphylococcus argenteus Clinical Isolates in Japan: Identification of Three Clones (ST1223, ST2198, and ST2550) and a Novel Staphylocoagulase Genotype XV
    Article Snippet: Phylogenetic Analysis of Virulence Factors Nucleotide sequences of full-length ORF were determined for staphylocoagulase genes belonging to a novel type, microbial surface component recognizing adhesive matrix molecule (MSCRAMM) family adhesin SdrE gene (sdrE ), elastin-binding protein gene (ebpS ), SE(-like) genes (selw , selx , sey , selz , sel26 , sel27 ), and staphylokinase gene (sak ) by PCR with primers listed in , followed by Sanger sequencing using the BigDye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) on an automated DNA sequencer (ABI PRISM 3100). .. Similarly, full length sequences of enterotoxin gene sec and tst-1 gene were also determined.

    Article Title: Salmonella in Retail Food and Wild Birds in Singapore—Prevalence, Antimicrobial Resistance, and Sequence Types
    Article Snippet: The PCR protocol was as follows: initial denaturation at 98 °C for 30 s, followed by 35 cycles of 98 °C for 10 sec, 55 °C for 30 sec, 72 °C for 30 s, with a final extension at 72 °C for 10 min. .. The amplified fragments were visualised on 2% agarose gels and subsequently; purified and sequenced using BigDye® Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Waltham, MA, USA).

    Article Title: Known and novel mutations responsible for epidermolysis bullosa simplex cases in a Chinese population
    Article Snippet: The following thermocycling conditions were used: Initial denaturation at 94°C for 5 min; 31 cycles of denaturation at 94°C for 30 sec, annealing (temperature for each primer is listed in ) for 30 sec, extension at 72°C for 1 min, and a final extension at 72°C for 1 min; and 4°C for 5 min. PCR products were purified with AxyPrep DNA Gel Extraction kit (Corning, Inc.) according to the manufacturer's instructions. .. The Sequencing Reaction system was based on BigDye® Terminator v3.1 Cycle Sequencing kit (Thermo Fisher Scientific, Inc.) according to the manufacturer's instructions.

    Purification:

    Article Title: Genotoxin Induced Mutagenesis in the Model Plant Physcomitrella patens
    Article Snippet: 71086), purified with the QIAquick PCR Purification Kit (Qiagen, cat. Nr. .. 28104) and used as a template for sequencing with BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, cat. Nr.

    Article Title: Rapidly declining trend of signet ring cell cancer of the stomach may parallel the infection rate of Helicobacter pylori
    Article Snippet: PCR products were purified using AMPure XP (Beckman Coulter) according to the manufacturer’s instructions. .. Sanger sequencing was performed with BigDye Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific) using forward or reverse primers.

    Article Title: A cardiac and subcutaneous canine dirofilariosis outbreak in a kennel in central France
    Article Snippet: .. The DNA generated through the PCR reaction was purified by filtration using a NucleoFast® 96 PCR DNA purification plate, and was then amplified using a BigDye® Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA). .. The BigDye PCR products were purified on the Sephadex G-50 Superfine gel filtration resin prior to sequencing on the ABI Prism 3130XL.

    Article Title: Highly Predictive Genetic Markers Distinguish Drug-Type from Fiber-Type Cannabis sativa L
    Article Snippet: .. Sequencing was conducted using a BigDye Terminator v3.1 Cycle Sequencing Kit (Life Technologies, Carlsbad, CA, USA) as follows: 4 μL reaction mix, 3.2 pmol of primer, and 2 μL of the purified PCR product in 15 μL total volume. .. The sequences were purified with a BigDye XTerminator Purification Kit and analyzed with an ABI PRISM 3130 Genetic Analyzer (Applied Biosystems).

    Article Title: Accurate detection of KRAS, NRAS and BRAF mutations in metastatic colorectal cancers by bridged nucleic acid-clamp real-time PCR
    Article Snippet: .. Purified products were used as templates and Sanger sequencing was performed using the BNA Real-time PCR Extended RAS Mutation Sequencing Primer (Riken Genesis) and the BigDye® Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific). .. Sequencing reactions consisted of 1.0 μL template PCR product, 0.5 μL 3.2 μM forward primer or 0.5 μL 3.2 μM reverse primer, 2 μL Big Dye Buffer, 1 μL BigDye v3.1 and 5.5 μL nuclease free water.

    Article Title: Salmonella in Retail Food and Wild Birds in Singapore—Prevalence, Antimicrobial Resistance, and Sequence Types
    Article Snippet: .. The amplified fragments were visualised on 2% agarose gels and subsequently; purified and sequenced using BigDye® Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Waltham, MA, USA). .. Raw sequences were then assembled in Lasergene Software version 8.0 (DNASTAR, Madison, WI, USA) and the consensus sequences were compared with those available in the MLST database ( http://enterobase.warwick.ac.uk/species/senterica/allele_st_search ) to determine allelic numbers and sequence types.

    Article Title: Known and novel mutations responsible for epidermolysis bullosa simplex cases in a Chinese population
    Article Snippet: The following thermocycling conditions were used: Initial denaturation at 94°C for 5 min; 31 cycles of denaturation at 94°C for 30 sec, annealing (temperature for each primer is listed in ) for 30 sec, extension at 72°C for 1 min, and a final extension at 72°C for 1 min; and 4°C for 5 min. PCR products were purified with AxyPrep DNA Gel Extraction kit (Corning, Inc.) according to the manufacturer's instructions. .. The Sequencing Reaction system was based on BigDye® Terminator v3.1 Cycle Sequencing kit (Thermo Fisher Scientific, Inc.) according to the manufacturer's instructions.

    Article Title: Impact of Preexisting Hepatitis C Virus Genotype 6 NS3, NS5A, and NS5B Polymorphisms on the In Vitro Potency of Direct-Acting Antiviral Agents
    Article Snippet: Viral RNA purification, cDNA synthesis, and PCR amplification were performed as previously described ( ). .. The NS3, NS5A, and NS5B coding regions were amplified, population-based sequencing (threshold of ≥15% for reporting polymorphisms at each residue position) was performed using the BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems, Foster City, CA), and genotype-specific primers were selected from the list in Table S1 in the supplemental material.

    Article Title: Design of N-Terminal Derivatives from a Novel Dermaseptin Exhibiting Broad-Spectrum Antimicrobial Activity against Isolates from Cystic Fibrosis Patients
    Article Snippet: The cDNA was amplified via RACE techniques and the purified products were cloned by pGEM-T easy vector system (Promega, Madison, W1, USA). .. Finally, each clone was sequenced by ABI 3100 automated capillary sequencer (Applied Biosystems, Foster City, CA, USA) using BigDye Terminator v3.1 Cycle Sequencing Kit (Life Technologies, Paisley, UK).

    Polymerase Chain Reaction:

    Article Title: Genotoxin Induced Mutagenesis in the Model Plant Physcomitrella patens
    Article Snippet: 71086), purified with the QIAquick PCR Purification Kit (Qiagen, cat. Nr. .. 28104) and used as a template for sequencing with BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, cat. Nr.

    Article Title: Molecular Epidemiological Characterization of Staphylococcus argenteus Clinical Isolates in Japan: Identification of Three Clones (ST1223, ST2198, and ST2550) and a Novel Staphylocoagulase Genotype XV
    Article Snippet: .. Phylogenetic Analysis of Virulence Factors Nucleotide sequences of full-length ORF were determined for staphylocoagulase genes belonging to a novel type, microbial surface component recognizing adhesive matrix molecule (MSCRAMM) family adhesin SdrE gene (sdrE ), elastin-binding protein gene (ebpS ), SE(-like) genes (selw , selx , sey , selz , sel26 , sel27 ), and staphylokinase gene (sak ) by PCR with primers listed in , followed by Sanger sequencing using the BigDye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) on an automated DNA sequencer (ABI PRISM 3100). .. Similarly, full length sequences of enterotoxin gene sec and tst-1 gene were also determined.

    Article Title: Rapidly declining trend of signet ring cell cancer of the stomach may parallel the infection rate of Helicobacter pylori
    Article Snippet: PCR products were purified using AMPure XP (Beckman Coulter) according to the manufacturer’s instructions. .. Sanger sequencing was performed with BigDye Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific) using forward or reverse primers.

    Article Title: A single-nucleotide polymorphism influences brain morphology in drug-naïve patients with major depressive disorder
    Article Snippet: .. Genotyping was carried out with a PCR SNP genotyping system using the BigDye Terminator v3.1 Cycle Sequencing Kit (Life Technologies Corporation, Tokyo, Japan). .. The DNA was read using a BMG Applied Biosystem 3730xI DNA Analyzer (Life Technologies Corporation).

    Article Title: A cardiac and subcutaneous canine dirofilariosis outbreak in a kennel in central France
    Article Snippet: .. The DNA generated through the PCR reaction was purified by filtration using a NucleoFast® 96 PCR DNA purification plate, and was then amplified using a BigDye® Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA). .. The BigDye PCR products were purified on the Sephadex G-50 Superfine gel filtration resin prior to sequencing on the ABI Prism 3130XL.

    Article Title: Highly Predictive Genetic Markers Distinguish Drug-Type from Fiber-Type Cannabis sativa L
    Article Snippet: .. Sequencing was conducted using a BigDye Terminator v3.1 Cycle Sequencing Kit (Life Technologies, Carlsbad, CA, USA) as follows: 4 μL reaction mix, 3.2 pmol of primer, and 2 μL of the purified PCR product in 15 μL total volume. .. The sequences were purified with a BigDye XTerminator Purification Kit and analyzed with an ABI PRISM 3130 Genetic Analyzer (Applied Biosystems).

    Article Title: Natural Human Infections With Plasmodium cynomolgi and Other Malaria Species in an Elimination Setting in Sabah, Malaysia
    Article Snippet: .. The amplified 18S rRNA gene was subsequently sequenced on the ABI 3730xl DNA analyzer, using the BigDye terminator v3.1 Cycle Sequencing Kit (Applied BioSystems) and primers from nest 2 of the 18s rRNA PCR. ..

    Article Title: Accurate detection of KRAS, NRAS and BRAF mutations in metastatic colorectal cancers by bridged nucleic acid-clamp real-time PCR
    Article Snippet: BNA-clamp PCR products were purified using ExoSAP-IT Express PCR Cleanup Reagent (Thermo Fisher Scientific) [ , ]. .. Purified products were used as templates and Sanger sequencing was performed using the BNA Real-time PCR Extended RAS Mutation Sequencing Primer (Riken Genesis) and the BigDye® Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific).

    Article Title: Salmonella in Retail Food and Wild Birds in Singapore—Prevalence, Antimicrobial Resistance, and Sequence Types
    Article Snippet: The PCR protocol was as follows: initial denaturation at 98 °C for 30 s, followed by 35 cycles of 98 °C for 10 sec, 55 °C for 30 sec, 72 °C for 30 s, with a final extension at 72 °C for 10 min. .. The amplified fragments were visualised on 2% agarose gels and subsequently; purified and sequenced using BigDye® Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Waltham, MA, USA).

    Article Title: Known and novel mutations responsible for epidermolysis bullosa simplex cases in a Chinese population
    Article Snippet: Paragraph title: PCR and Sanger sequencing ... The Sequencing Reaction system was based on BigDye® Terminator v3.1 Cycle Sequencing kit (Thermo Fisher Scientific, Inc.) according to the manufacturer's instructions.

    Article Title: Impact of Preexisting Hepatitis C Virus Genotype 6 NS3, NS5A, and NS5B Polymorphisms on the In Vitro Potency of Direct-Acting Antiviral Agents
    Article Snippet: Viral RNA purification, cDNA synthesis, and PCR amplification were performed as previously described ( ). .. The NS3, NS5A, and NS5B coding regions were amplified, population-based sequencing (threshold of ≥15% for reporting polymorphisms at each residue position) was performed using the BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems, Foster City, CA), and genotype-specific primers were selected from the list in Table S1 in the supplemental material.

    Article Title: Histology and molecular biology studies on the expression and localization of angiopoietin-like protein 8 in human tissues
    Article Snippet: The amplified PCR product was cloned into pCR-II TOPO (Thermo Fisher Scientific, Inc.) using the TA-cloning method. .. Cloned ANGPTL8 fragments were sequenced using the BigDye Terminator v3.1 Cycle Sequencing kit (Thermo Fisher Scientific, Inc.).

    Gel Extraction:

    Article Title: Known and novel mutations responsible for epidermolysis bullosa simplex cases in a Chinese population
    Article Snippet: The following thermocycling conditions were used: Initial denaturation at 94°C for 5 min; 31 cycles of denaturation at 94°C for 30 sec, annealing (temperature for each primer is listed in ) for 30 sec, extension at 72°C for 1 min, and a final extension at 72°C for 1 min; and 4°C for 5 min. PCR products were purified with AxyPrep DNA Gel Extraction kit (Corning, Inc.) according to the manufacturer's instructions. .. The Sequencing Reaction system was based on BigDye® Terminator v3.1 Cycle Sequencing kit (Thermo Fisher Scientific, Inc.) according to the manufacturer's instructions.

    cDNA Library Assay:

    Article Title: Design of N-Terminal Derivatives from a Novel Dermaseptin Exhibiting Broad-Spectrum Antimicrobial Activity against Isolates from Cystic Fibrosis Patients
    Article Snippet: Identification of DM-PC Precursor-Encoding cDNA from Skin Secretion The mRNA from skin secretion was isolated and skin secretion-derived cDNA library of Phyllomedusa coelestis was constructed as described previously [ ]. .. Finally, each clone was sequenced by ABI 3100 automated capillary sequencer (Applied Biosystems, Foster City, CA, USA) using BigDye Terminator v3.1 Cycle Sequencing Kit (Life Technologies, Paisley, UK).

    Multiplex Ligation-dependent Probe Amplification:

    Article Title: Investigation on the hereditary basis of colorectal cancers in an African population with frequent early onset cases
    Article Snippet: Sequencing was performed using BigDye terminator v3.1 cycle sequencing kit (Thermofisher Scientific, Wilmington, USA) following standard protocols. .. MLPA for MLH1 , MSH2 and EPCAM germline deletions was performed using the SALSA MLPA kit (MRC-Holland, Amsterdam, Netherlands) according to the manufacturer’s protocol.

    Plasmid Preparation:

    Article Title: Design of N-Terminal Derivatives from a Novel Dermaseptin Exhibiting Broad-Spectrum Antimicrobial Activity against Isolates from Cystic Fibrosis Patients
    Article Snippet: The cDNA was amplified via RACE techniques and the purified products were cloned by pGEM-T easy vector system (Promega, Madison, W1, USA). .. Finally, each clone was sequenced by ABI 3100 automated capillary sequencer (Applied Biosystems, Foster City, CA, USA) using BigDye Terminator v3.1 Cycle Sequencing Kit (Life Technologies, Paisley, UK).

    Software:

    Article Title: Molecular Epidemiological Characterization of Staphylococcus argenteus Clinical Isolates in Japan: Identification of Three Clones (ST1223, ST2198, and ST2550) and a Novel Staphylocoagulase Genotype XV
    Article Snippet: Phylogenetic Analysis of Virulence Factors Nucleotide sequences of full-length ORF were determined for staphylocoagulase genes belonging to a novel type, microbial surface component recognizing adhesive matrix molecule (MSCRAMM) family adhesin SdrE gene (sdrE ), elastin-binding protein gene (ebpS ), SE(-like) genes (selw , selx , sey , selz , sel26 , sel27 ), and staphylokinase gene (sak ) by PCR with primers listed in , followed by Sanger sequencing using the BigDye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) on an automated DNA sequencer (ABI PRISM 3100). .. Phylogenetic dendrograms of these virulence factors were constructed by the maximum likelihood method using the MEGA7 software, together with sequence data of staphylococcal strains available in GenBank database.

    Article Title: Salmonella in Retail Food and Wild Birds in Singapore—Prevalence, Antimicrobial Resistance, and Sequence Types
    Article Snippet: The amplified fragments were visualised on 2% agarose gels and subsequently; purified and sequenced using BigDye® Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Waltham, MA, USA). .. Raw sequences were then assembled in Lasergene Software version 8.0 (DNASTAR, Madison, WI, USA) and the consensus sequences were compared with those available in the MLST database ( http://enterobase.warwick.ac.uk/species/senterica/allele_st_search ) to determine allelic numbers and sequence types.

    Article Title: Investigation on the hereditary basis of colorectal cancers in an African population with frequent early onset cases
    Article Snippet: Sequencing was performed using BigDye terminator v3.1 cycle sequencing kit (Thermofisher Scientific, Wilmington, USA) following standard protocols. .. The amplified products were visualised using capillary electrophoresis, and the output analysed using the Coffalyser.Net software ( https://www.mlpa.com ).

    Real-time Polymerase Chain Reaction:

    Article Title: Inherited Variants in BLM and the Risk and Clinical Characteristics of Breast Cancer
    Article Snippet: The c.1642C > T (p.Gln548Ter) mutation was genotyped using a TaqMan assay (Thermo Fisher Scientific, Waltham, MA, USA) in a LightCycler Real-Time PCR 480 System (Roche Life Science, Mannheim, Germany). .. Sequencing reactions were performed using a BigDye Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol.

    Article Title: Accurate detection of KRAS, NRAS and BRAF mutations in metastatic colorectal cancers by bridged nucleic acid-clamp real-time PCR
    Article Snippet: .. Purified products were used as templates and Sanger sequencing was performed using the BNA Real-time PCR Extended RAS Mutation Sequencing Primer (Riken Genesis) and the BigDye® Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific). .. Sequencing reactions consisted of 1.0 μL template PCR product, 0.5 μL 3.2 μM forward primer or 0.5 μL 3.2 μM reverse primer, 2 μL Big Dye Buffer, 1 μL BigDye v3.1 and 5.5 μL nuclease free water.

    Multiplex Assay:

    Article Title: Highly Predictive Genetic Markers Distinguish Drug-Type from Fiber-Type Cannabis sativa L
    Article Snippet: The amplification reaction, conducted using a Qiagen Multiplex PCR Kit, was performed in a final volume of 25 μL using 10 μM primers and 5 ng of DNA. .. Sequencing was conducted using a BigDye Terminator v3.1 Cycle Sequencing Kit (Life Technologies, Carlsbad, CA, USA) as follows: 4 μL reaction mix, 3.2 pmol of primer, and 2 μL of the purified PCR product in 15 μL total volume.

    Agarose Gel Electrophoresis:

    Article Title: Rapidly declining trend of signet ring cell cancer of the stomach may parallel the infection rate of Helicobacter pylori
    Article Snippet: Reactions were subject to thermal cycling with the following conditions: 94 °C for 2 min, 45 cycles of 94 °C for 15 s, 60 °C for 15 s, and 68 °C for 30 s. To determine the H. pylori- specific amplicon, 5 μL of the PCR products was separated by electrophoresis on 2% agarose gel, stained with ethidium bromide and visualized on an FAS IV UV Illuminator (NIPPON Genetics, Japan) [ , ]. .. Sanger sequencing was performed with BigDye Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific) using forward or reverse primers.

    Article Title: Highly Predictive Genetic Markers Distinguish Drug-Type from Fiber-Type Cannabis sativa L
    Article Snippet: The amplified products were loaded on a 2% agarose gel in 1X TBE. .. Sequencing was conducted using a BigDye Terminator v3.1 Cycle Sequencing Kit (Life Technologies, Carlsbad, CA, USA) as follows: 4 μL reaction mix, 3.2 pmol of primer, and 2 μL of the purified PCR product in 15 μL total volume.

    DNA Purification:

    Article Title: A cardiac and subcutaneous canine dirofilariosis outbreak in a kennel in central France
    Article Snippet: .. The DNA generated through the PCR reaction was purified by filtration using a NucleoFast® 96 PCR DNA purification plate, and was then amplified using a BigDye® Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA). .. The BigDye PCR products were purified on the Sephadex G-50 Superfine gel filtration resin prior to sequencing on the ABI Prism 3130XL.

    Staining:

    Article Title: Rapidly declining trend of signet ring cell cancer of the stomach may parallel the infection rate of Helicobacter pylori
    Article Snippet: Reactions were subject to thermal cycling with the following conditions: 94 °C for 2 min, 45 cycles of 94 °C for 15 s, 60 °C for 15 s, and 68 °C for 30 s. To determine the H. pylori- specific amplicon, 5 μL of the PCR products was separated by electrophoresis on 2% agarose gel, stained with ethidium bromide and visualized on an FAS IV UV Illuminator (NIPPON Genetics, Japan) [ , ]. .. Sanger sequencing was performed with BigDye Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific) using forward or reverse primers.

    Article Title: Highly Predictive Genetic Markers Distinguish Drug-Type from Fiber-Type Cannabis sativa L
    Article Snippet: After being stained with ethidium bromide, the amplified products were photographed under UV light (254 nm). .. Sequencing was conducted using a BigDye Terminator v3.1 Cycle Sequencing Kit (Life Technologies, Carlsbad, CA, USA) as follows: 4 μL reaction mix, 3.2 pmol of primer, and 2 μL of the purified PCR product in 15 μL total volume.

    Article Title: Investigation on the hereditary basis of colorectal cancers in an African population with frequent early onset cases
    Article Snippet: The stained slides were interpreted by an experienced pathologist (DG). .. Sequencing was performed using BigDye terminator v3.1 cycle sequencing kit (Thermofisher Scientific, Wilmington, USA) following standard protocols.

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    Thermo Fisher bigdye terminater v3 1 cycle sequencing kit
    Bigdye Terminater V3 1 Cycle Sequencing Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bigdye terminater v3 1 cycle sequencing kit/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
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    bigdye terminater v3 1 cycle sequencing kit - by Bioz Stars, 2020-01
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