bigdye terminator cycle sequencing kit (Thermo Fisher)
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Thermo Fisher
bigdye terminator cycle sequencing kit
Bigdye Terminator Cycle Sequencing Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bigdye terminator cycle sequencing kit/product/Thermo Fisher
Average 99 stars, based on 20 article reviews
Price from $9.99 to $1999.99
Bigdye Terminator Cycle Sequencing Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bigdye terminator cycle sequencing kit/product/Thermo Fisher
Average 99 stars, based on 20 article reviews
Price from $9.99 to $1999.99
bigdye terminator cycle sequencing kit - by Bioz Stars,
2020-01
99/100 stars
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Centrifugation:Article Title: A purely quantitative form of partial recessive IFN-óR2 deficiency caused by mutations of the initiation or second codon Article Snippet: .. The PCR products were purified by centrifugation through Sephadex G-50 Superfine resin (GE Healthcare) and sequenced with the Amplification:Article Title: Significant association of rare variant p.Gly8Ser in cardiac sodium channel β4-subunit SCN4B with atrial fibrillation Article Snippet: All exons and exon–intron boundaries were amplified using polymerase chain reactions using the primers listed in . .. DNA sequencing analysis was performed using a Article Title: The genus Massalongia (lichenised ascomycetae) in the Southern Hemisphere Article Snippet: Paragraph title: DNA extraction, Amplification and Sequencing ... Direct sequencing of PCR products was run with the PCR primers using a Article Title: CRISPR-Cas3 induces broad and unidirectional genome editing in human cells Article Snippet: The targeted regions were amplified using Quick Taq HS DyeMix (Toyobo), KOD FX Neo (Toyobo) or Gflex (Takara Bio) and electrophoresed on an agarose gel or Agilent 2200 Tape Station (Agilent Technologies). .. Sequencing analysis was performed using the Article Title: Five major sequence variants and copy number variants in the EYS gene account for one-third of Japanese patients with autosomal recessive and simplex retinitis pigmentosa Article Snippet: .. Two hundred nanograms of genomic DNA were amplified with Taq polymerase (TaKaRa PrimeSTAR; Takara Bio Inc., Shiga, Japan), and a sequence variant analysis was performed with direct sequencing of the purified PCR products ( Article Title: The co-occurrence of rare non-ocular phenotypes in patients with inherited retinal degenerations Article Snippet: Testing for specific mutations and verification of mutations identified with WES were performed by PCR amplification with specifically designed primers. .. This was followed by direct sequencing with the Article Title: A novel sequestrate species from Mexico: Aroramycesguanajuatensis sp. nov. ( Hysterangiaceae, Hysterangiales) Article Snippet: Paragraph title: DNA extraction, amplification, sequencing and phylogenetic analyses ... Finally, the products were purified using the ExoSap Kit (Affymetrix, USA) according to the manufacturer’s instructions and were prepared for the sequencing reaction using the Article Title: Newborn screening for cerebrotendinous xanthomatosis is the solution for early identification and treatment Article Snippet: For Moroccan Jews, all three known pathogenic genetic variants were tested; the c.1016 C > T (pThr339Met) variant by amplification of a 382 bp fragment from exon 5 in CYP27A1 using sense primer (5′-TGCTTTCACAGGGAAGAAGC-3′) and antisense primer (5′-ACAAAAGGACCATCCCCTGT-3′). .. Sequences were determined using the Article Title: Necrotic and Cytolytic Activity on Grapevine Leaves Produced by Nep1-Like Proteins of Diplodia seriata Article Snippet: Paragraph title: PCR Amplification and Sequencing of DserNEP Genes ... The program consisted of an initial step of 2 min at 95°C, followed by 35 cycles of denaturation at 95°C for 20 s, annealing at 60°C for 15 s, and elongation at 72°C for 30 s. A final extension was performed at 72°C for 3 min. DNA was sequenced by the dideoxynucleotide chain termination method using a Article Title: Identification of rare variants in cardiac sodium channel β4‑subunit gene SCN4B associated with ventricular tachycardia Article Snippet: All exons and exon–intron boundaries were amplified using polymerase chain reactions (PCR) and subjected to high-resolution melt analysis (HRM) as described ( , ). .. DNA sequencing was performed using TA Cloning:Article Title: CRISPR-Cas3 induces broad and unidirectional genome editing in human cells Article Snippet: For TA cloning, the PCR products were purified using a DNA Clean Up Kit and subcloned into a pCR4Blunt-TOPO plasmid vector (Thermo Fisher Scientific) according to the manufacturer’s protocol. .. Sequencing analysis was performed using the Real-time Polymerase Chain Reaction:Article Title: Investigation of urban birds as source of β-lactamase-producing Gram-negative bacteria in Marseille city, France Article Snippet: On overall, by qPCR and standard PCR we investigated genes encoding for extend-spectrum-β-lactamases (bla SHV , bla VEB , bla GES , bla PER ), carbapenemases (VIM, NDM, OXA-23, OXA-24, OXA-48, OXA-58), and mobile colistin resistance genes (mcr -1 to mcr -5 ) using primers and probes previously reported [ ]. .. Sequencing was performed using the Article Title: Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens, et al. Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens Article Snippet: PCR was performed in a total volume of 20 μL (10 μL PCR reaction mixture, 5 μL probe mixture, and 5 μL DNA) using the 7500 Fast Real‐Time PCR System. .. After detection of PCR products by gel electrophoresis and purification, sequencing was performed on an automated ABI Prism 3730 instrument using a Microarray:Article Title: The co-occurrence of rare non-ocular phenotypes in patients with inherited retinal degenerations Article Snippet: Chromosomal microarray analysis (CMA) in family 2 was performed using the CytoScanTM 750K Chip Kit (PE Applied Biosystems, Foster City, CA). .. This was followed by direct sequencing with the Expressing:Article Title: CRISPR-Cas3 induces broad and unidirectional genome editing in human cells Article Snippet: Detection of large deletion mutations in human cells At 24 h after seeding 5.0 × 104 293T cells in each well of a 24-well plate, 250 ng each of Cas3, Cas5, Cas6, Cas7, Cas8, Cas11, and crRNA expression vectors were transfected into 293T cells as described above. .. Sequencing analysis was performed using the Transfection:Article Title: CRISPR-Cas3 induces broad and unidirectional genome editing in human cells Article Snippet: Two days after the transfection, whole DNA was extracted from collected cells using the Tissue XS Kit (Takara Bio) according to the manufacturer’s protocol. .. Sequencing analysis was performed using the Cell Culture:Article Title: Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens, et al. Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens Article Snippet: After detection of PCR products by gel electrophoresis and purification, sequencing was performed on an automated ABI Prism 3730 instrument using a Generated:Article Title: The genus Massalongia (lichenised ascomycetae) in the Southern Hemisphere Article Snippet: Direct sequencing of PCR products was run with the PCR primers using a DNA Sequencing:Article Title: Significant association of rare variant p.Gly8Ser in cardiac sodium channel β4-subunit SCN4B with atrial fibrillation Article Snippet: .. DNA sequencing analysis was performed using a Article Title: An enhancer cluster controls gene activity and topology of the SCN5A-SCN10A locus in vivo Article Snippet: .. Sanger sequencing DNA sequencing was performed with Article Title: The genus Massalongia (lichenised ascomycetae) in the Southern Hemisphere Article Snippet: .. Direct sequencing of PCR products was run with the PCR primers using a Article Title: High‐Resolution Melting Analysis as a Developed Method for Genotyping the PD Susceptibility Loci in LRRK2 Gene Article Snippet: .. The above PCR products from R1628P locus were purified, and then direct DNA sequencing was performed using the Article Title: Identification of rare variants in cardiac sodium channel β4‑subunit gene SCN4B associated with ventricular tachycardia Article Snippet: .. DNA sequencing was performed using Sequencing:Article Title: Investigation of urban birds as source of β-lactamase-producing Gram-negative bacteria in Marseille city, France Article Snippet: .. Sequencing was performed using the Article Title: Significant association of rare variant p.Gly8Ser in cardiac sodium channel β4-subunit SCN4B with atrial fibrillation Article Snippet: .. DNA sequencing analysis was performed using a Article Title: An enhancer cluster controls gene activity and topology of the SCN5A-SCN10A locus in vivo Article Snippet: .. Sanger sequencing DNA sequencing was performed with Article Title: The genus Massalongia (lichenised ascomycetae) in the Southern Hemisphere Article Snippet: .. Direct sequencing of PCR products was run with the PCR primers using a Article Title: CRISPR-Cas3 induces broad and unidirectional genome editing in human cells Article Snippet: .. Sequencing analysis was performed using the Article Title: Five major sequence variants and copy number variants in the EYS gene account for one-third of Japanese patients with autosomal recessive and simplex retinitis pigmentosa Article Snippet: .. Two hundred nanograms of genomic DNA were amplified with Taq polymerase (TaKaRa PrimeSTAR; Takara Bio Inc., Shiga, Japan), and a sequence variant analysis was performed with direct sequencing of the purified PCR products ( Article Title: The co-occurrence of rare non-ocular phenotypes in patients with inherited retinal degenerations Article Snippet: .. This was followed by direct sequencing with the Article Title: High‐Resolution Melting Analysis as a Developed Method for Genotyping the PD Susceptibility Loci in LRRK2 Gene Article Snippet: .. The above PCR products from R1628P locus were purified, and then direct DNA sequencing was performed using the Article Title: Global Assessment of Mycobacterium avium subsp. hominissuis Genetic Requirement for Growth and Virulence Article Snippet: .. Sanger sequencing was performed using genomic DNA (gDNA) as the template prepared with Article Title: A novel sequestrate species from Mexico: Aroramycesguanajuatensis sp. nov. ( Hysterangiaceae, Hysterangiales) Article Snippet: .. Finally, the products were purified using the ExoSap Kit (Affymetrix, USA) according to the manufacturer’s instructions and were prepared for the sequencing reaction using the Article Title: A purely quantitative form of partial recessive IFN-óR2 deficiency caused by mutations of the initiation or second codon Article Snippet: .. The PCR products were purified by centrifugation through Sephadex G-50 Superfine resin (GE Healthcare) and sequenced with the Article Title: Newborn screening for cerebrotendinous xanthomatosis is the solution for early identification and treatment Article Snippet: .. Sequences were determined using the Article Title: Necrotic and Cytolytic Activity on Grapevine Leaves Produced by Nep1-Like Proteins of Diplodia seriata Article Snippet: .. The program consisted of an initial step of 2 min at 95°C, followed by 35 cycles of denaturation at 95°C for 20 s, annealing at 60°C for 15 s, and elongation at 72°C for 30 s. A final extension was performed at 72°C for 3 min. DNA was sequenced by the dideoxynucleotide chain termination method using a Article Title: Identification of rare variants in cardiac sodium channel β4‑subunit gene SCN4B associated with ventricular tachycardia Article Snippet: .. DNA sequencing was performed using Article Title: Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens, et al. Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens Article Snippet: .. After detection of PCR products by gel electrophoresis and purification, sequencing was performed on an automated ABI Prism 3730 instrument using a DNA Extraction:Article Title: The genus Massalongia (lichenised ascomycetae) in the Southern Hemisphere Article Snippet: Paragraph title: DNA extraction, Amplification and Sequencing ... Direct sequencing of PCR products was run with the PCR primers using a Article Title: Five major sequence variants and copy number variants in the EYS gene account for one-third of Japanese patients with autosomal recessive and simplex retinitis pigmentosa Article Snippet: Sequencing analysis Genomic DNA was isolated from venous blood using a DNA purification kit (Puregene; Gentra Systems, Minneapolis, MN) for the subjects of NRCD and a DNA extraction kit (Quick-Gene-610L; Fujifilm, Tokyo, Japan) for the patients of Kyoto University. .. Two hundred nanograms of genomic DNA were amplified with Taq polymerase (TaKaRa PrimeSTAR; Takara Bio Inc., Shiga, Japan), and a sequence variant analysis was performed with direct sequencing of the purified PCR products ( Article Title: Global Assessment of Mycobacterium avium subsp. hominissuis Genetic Requirement for Growth and Virulence Article Snippet: Total DNA was isolated using Masterpure Complete DNA Isolation kit (Epicentre). .. Sanger sequencing was performed using genomic DNA (gDNA) as the template prepared with Article Title: A novel sequestrate species from Mexico: Aroramycesguanajuatensis sp. nov. ( Hysterangiaceae, Hysterangiales) Article Snippet: Paragraph title: DNA extraction, amplification, sequencing and phylogenetic analyses ... Finally, the products were purified using the ExoSap Kit (Affymetrix, USA) according to the manufacturer’s instructions and were prepared for the sequencing reaction using the Article Title: Newborn screening for cerebrotendinous xanthomatosis is the solution for early identification and treatment Article Snippet: Paragraph title: DNA extraction from DBSs and genetic analysis ... Sequences were determined using the Nucleic Acid Electrophoresis:Article Title: Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens, et al. Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens Article Snippet: .. After detection of PCR products by gel electrophoresis and purification, sequencing was performed on an automated ABI Prism 3730 instrument using a Mutagenesis:Article Title: CRISPR-Cas3 induces broad and unidirectional genome editing in human cells Article Snippet: To detect small indel mutations in the PCR products, T7 endonuclease I (New England Biolabs) or the SURVEYOR Mutation Detection Kit (Integrated DNA Technologies) was used in accordance with the manufacturer’s protocol. .. Sequencing analysis was performed using the Isolation:Article Title: Five major sequence variants and copy number variants in the EYS gene account for one-third of Japanese patients with autosomal recessive and simplex retinitis pigmentosa Article Snippet: Genomic DNA of family members was isolated from their saliva using a DNA self-collection kit (Oragene® -DNA OG-500; DNA Genotek, Ottawa, Canada). .. Two hundred nanograms of genomic DNA were amplified with Taq polymerase (TaKaRa PrimeSTAR; Takara Bio Inc., Shiga, Japan), and a sequence variant analysis was performed with direct sequencing of the purified PCR products ( Article Title: Global Assessment of Mycobacterium avium subsp. hominissuis Genetic Requirement for Growth and Virulence Article Snippet: Total DNA was isolated using Masterpure Complete DNA Isolation kit (Epicentre). .. Sanger sequencing was performed using genomic DNA (gDNA) as the template prepared with Size-exclusion Chromatography:Article Title: The genus Massalongia (lichenised ascomycetae) in the Southern Hemisphere Article Snippet: PCR reactions were performed on a C1000 Touch thermal cycler (Bio-Rad Laboratories), with the following temperatures; initial denaturation at 94 °C for 4 min, followed by a 62–56 °C touchdown annealing for the first 6 cycles, ending with 30 cycles at 56 °C for 30 sec, polymerisation at 72 °C for 1 min 45 sec and a final elongation at 72 °C for 10 min. .. Direct sequencing of PCR products was run with the PCR primers using a Purification:Article Title: Investigation of urban birds as source of β-lactamase-producing Gram-negative bacteria in Marseille city, France Article Snippet: All positive PCR products for genes encoding for ESBL that conferred resistance to 3rd generation cephalosporins were purified using the QIAquick PCR purification kit in concordance with the manufacturer’s instructions (Qiagen, Hilden, Germany). .. Sequencing was performed using the Article Title: CRISPR-Cas3 induces broad and unidirectional genome editing in human cells Article Snippet: For TA cloning, the PCR products were purified using a DNA Clean Up Kit and subcloned into a pCR4Blunt-TOPO plasmid vector (Thermo Fisher Scientific) according to the manufacturer’s protocol. .. Sequencing analysis was performed using the Article Title: Five major sequence variants and copy number variants in the EYS gene account for one-third of Japanese patients with autosomal recessive and simplex retinitis pigmentosa Article Snippet: .. Two hundred nanograms of genomic DNA were amplified with Taq polymerase (TaKaRa PrimeSTAR; Takara Bio Inc., Shiga, Japan), and a sequence variant analysis was performed with direct sequencing of the purified PCR products ( Article Title: High‐Resolution Melting Analysis as a Developed Method for Genotyping the PD Susceptibility Loci in LRRK2 Gene Article Snippet: .. The above PCR products from R1628P locus were purified, and then direct DNA sequencing was performed using the Article Title: Global Assessment of Mycobacterium avium subsp. hominissuis Genetic Requirement for Growth and Virulence Article Snippet: Sanger sequencing was performed using genomic DNA (gDNA) as the template prepared with Article Title: A novel sequestrate species from Mexico: Aroramycesguanajuatensis sp. nov. ( Hysterangiaceae, Hysterangiales) Article Snippet: .. Finally, the products were purified using the ExoSap Kit (Affymetrix, USA) according to the manufacturer’s instructions and were prepared for the sequencing reaction using the Article Title: A purely quantitative form of partial recessive IFN-óR2 deficiency caused by mutations of the initiation or second codon Article Snippet: .. The PCR products were purified by centrifugation through Sephadex G-50 Superfine resin (GE Healthcare) and sequenced with the Article Title: Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens, et al. Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens Article Snippet: .. After detection of PCR products by gel electrophoresis and purification, sequencing was performed on an automated ABI Prism 3730 instrument using a Polymerase Chain Reaction:Article Title: Investigation of urban birds as source of β-lactamase-producing Gram-negative bacteria in Marseille city, France Article Snippet: All positive PCR products for genes encoding for ESBL that conferred resistance to 3rd generation cephalosporins were purified using the QIAquick PCR purification kit in concordance with the manufacturer’s instructions (Qiagen, Hilden, Germany). .. Sequencing was performed using the Article Title: The genus Massalongia (lichenised ascomycetae) in the Southern Hemisphere Article Snippet: .. Direct sequencing of PCR products was run with the PCR primers using a Article Title: CRISPR-Cas3 induces broad and unidirectional genome editing in human cells Article Snippet: For TA cloning, the PCR products were purified using a DNA Clean Up Kit and subcloned into a pCR4Blunt-TOPO plasmid vector (Thermo Fisher Scientific) according to the manufacturer’s protocol. .. Sequencing analysis was performed using the Article Title: Five major sequence variants and copy number variants in the EYS gene account for one-third of Japanese patients with autosomal recessive and simplex retinitis pigmentosa Article Snippet: .. Two hundred nanograms of genomic DNA were amplified with Taq polymerase (TaKaRa PrimeSTAR; Takara Bio Inc., Shiga, Japan), and a sequence variant analysis was performed with direct sequencing of the purified PCR products ( Article Title: The co-occurrence of rare non-ocular phenotypes in patients with inherited retinal degenerations Article Snippet: PCR was performed on 50 ng of genomic DNA in a 25 µl reaction volume in the presence of 5X Readymix (LAROVA GmbH, Teltow, Germany) and 10 pmol of each forward and reverse primers. .. This was followed by direct sequencing with the Article Title: High‐Resolution Melting Analysis as a Developed Method for Genotyping the PD Susceptibility Loci in LRRK2 Gene Article Snippet: .. The above PCR products from R1628P locus were purified, and then direct DNA sequencing was performed using the Article Title: Global Assessment of Mycobacterium avium subsp. hominissuis Genetic Requirement for Growth and Virulence Article Snippet: .. Sanger sequencing was performed using genomic DNA (gDNA) as the template prepared with Article Title: A novel sequestrate species from Mexico: Aroramycesguanajuatensis sp. nov. ( Hysterangiaceae, Hysterangiales) Article Snippet: The PCR products were verified by agarose gel electrophoresis run for 1 h at 95 V cm-3 in 1.5% agarose and 1× TAE buffer (Tris Acetate-EDTA). .. Finally, the products were purified using the ExoSap Kit (Affymetrix, USA) according to the manufacturer’s instructions and were prepared for the sequencing reaction using the Article Title: A purely quantitative form of partial recessive IFN-óR2 deficiency caused by mutations of the initiation or second codon Article Snippet: .. The PCR products were purified by centrifugation through Sephadex G-50 Superfine resin (GE Healthcare) and sequenced with the Article Title: Newborn screening for cerebrotendinous xanthomatosis is the solution for early identification and treatment Article Snippet: The PCR products were digested with HpyCH4 III restriction enzyme (New England Biolabs) and analyzed on 3.5% agarose gels. .. Sequences were determined using the Article Title: Necrotic and Cytolytic Activity on Grapevine Leaves Produced by Nep1-Like Proteins of Diplodia seriata Article Snippet: Paragraph title: PCR Amplification and Sequencing of DserNEP Genes ... The program consisted of an initial step of 2 min at 95°C, followed by 35 cycles of denaturation at 95°C for 20 s, annealing at 60°C for 15 s, and elongation at 72°C for 30 s. A final extension was performed at 72°C for 3 min. DNA was sequenced by the dideoxynucleotide chain termination method using a Article Title: Identification of rare variants in cardiac sodium channel β4‑subunit gene SCN4B associated with ventricular tachycardia Article Snippet: The primers used to PCR and HRM analyses were designed using the primer-3 web site and listed in . .. DNA sequencing was performed using Article Title: Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens, et al. Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens Article Snippet: .. After detection of PCR products by gel electrophoresis and purification, sequencing was performed on an automated ABI Prism 3730 instrument using a Chromatin Immunoprecipitation:Article Title: The co-occurrence of rare non-ocular phenotypes in patients with inherited retinal degenerations Article Snippet: Chromosomal microarray analysis (CMA) in family 2 was performed using the CytoScanTM 750K Chip Kit (PE Applied Biosystems, Foster City, CA). .. This was followed by direct sequencing with the Plasmid Preparation:Article Title: CRISPR-Cas3 induces broad and unidirectional genome editing in human cells Article Snippet: For TA cloning, the PCR products were purified using a DNA Clean Up Kit and subcloned into a pCR4Blunt-TOPO plasmid vector (Thermo Fisher Scientific) according to the manufacturer’s protocol. .. Sequencing analysis was performed using the Software:Article Title: A purely quantitative form of partial recessive IFN-óR2 deficiency caused by mutations of the initiation or second codon Article Snippet: The PCR products were purified by centrifugation through Sephadex G-50 Superfine resin (GE Healthcare) and sequenced with the Agarose Gel Electrophoresis:Article Title: CRISPR-Cas3 induces broad and unidirectional genome editing in human cells Article Snippet: The targeted regions were amplified using Quick Taq HS DyeMix (Toyobo), KOD FX Neo (Toyobo) or Gflex (Takara Bio) and electrophoresed on an agarose gel or Agilent 2200 Tape Station (Agilent Technologies). .. Sequencing analysis was performed using the Article Title: A novel sequestrate species from Mexico: Aroramycesguanajuatensis sp. nov. ( Hysterangiaceae, Hysterangiales) Article Snippet: The PCR products were verified by agarose gel electrophoresis run for 1 h at 95 V cm-3 in 1.5% agarose and 1× TAE buffer (Tris Acetate-EDTA). .. Finally, the products were purified using the ExoSap Kit (Affymetrix, USA) according to the manufacturer’s instructions and were prepared for the sequencing reaction using the DNA Purification:Article Title: Five major sequence variants and copy number variants in the EYS gene account for one-third of Japanese patients with autosomal recessive and simplex retinitis pigmentosa Article Snippet: Sequencing analysis Genomic DNA was isolated from venous blood using a DNA purification kit (Puregene; Gentra Systems, Minneapolis, MN) for the subjects of NRCD and a DNA extraction kit (Quick-Gene-610L; Fujifilm, Tokyo, Japan) for the patients of Kyoto University. .. Two hundred nanograms of genomic DNA were amplified with Taq polymerase (TaKaRa PrimeSTAR; Takara Bio Inc., Shiga, Japan), and a sequence variant analysis was performed with direct sequencing of the purified PCR products ( Variant Assay:Article Title: Significant association of rare variant p.Gly8Ser in cardiac sodium channel β4-subunit SCN4B with atrial fibrillation Article Snippet: Paragraph title: 2.2 |. Mutational analysis of SCN4B and genotyping of variant p.Gly8Ser ... DNA sequencing analysis was performed using a Article Title: CRISPR-Cas3 induces broad and unidirectional genome editing in human cells Article Snippet: Sequencing analysis was performed using the Article Title: Five major sequence variants and copy number variants in the EYS gene account for one-third of Japanese patients with autosomal recessive and simplex retinitis pigmentosa Article Snippet: .. Two hundred nanograms of genomic DNA were amplified with Taq polymerase (TaKaRa PrimeSTAR; Takara Bio Inc., Shiga, Japan), and a sequence variant analysis was performed with direct sequencing of the purified PCR products ( Article Title: Newborn screening for cerebrotendinous xanthomatosis is the solution for early identification and treatment Article Snippet: For Moroccan Jews, all three known pathogenic genetic variants were tested; the c.1016 C > T (pThr339Met) variant by amplification of a 382 bp fragment from exon 5 in CYP27A1 using sense primer (5′-TGCTTTCACAGGGAAGAAGC-3′) and antisense primer (5′-ACAAAAGGACCATCCCCTGT-3′). .. Sequences were determined using the |