bigdye terminator cycle sequencing kit  (Thermo Fisher)


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    Name:
    BigDye Terminator v1 1 Cycle Sequencing Kit
    Description:
    This BigDye Terminator v1 1 Cycle Sequencing Kit is designed for specialty applications that require optimal basecalling adjacent to the primer and for sequencing short PCR product templates with rapid electrophoresis run modules • Improve the quality of your results for a wide range of sequencing applications • Sequence challenging templates and sequences more successfully • Get longer higher quality reads with more uniform peak heights and optimal signal balance • Enhance your productivity and reduce costs Tackle Tough Sequences With the BigDye Terminator v1 1 Cycle Sequencing Kit you get a robust highly flexible chemistry for a wide range of applications including de novo sequencing and resequencing Get More Uniform Peak Heights Improved AccuracyWith BigDye Terminator v1 1 kit s superior chemistry you generate data with uniform peak heights and optimized signal balance which results in longer higher quality reads You also get more accurate base assignments for heterozygote and mutation detection Easily Integrate into Your WorkflowBigDye Terminator v 1 1 kit s chemistry requires no new software or instrument recalibration You can easily integrate either kit into your current workflow with minimal changes to your protocols For Research Use Only Not for use in diagnostics procedures
    Catalog Number:
    4337449
    Price:
    None
    Applications:
    Kits & Reagents for Sanger Sequencing|Sanger Sequencing|Sanger Sequencing Technology & Accessories|Sequencing
    Category:
    Kits and Assays
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    Structured Review

    Thermo Fisher bigdye terminator cycle sequencing kit
    This BigDye Terminator v1 1 Cycle Sequencing Kit is designed for specialty applications that require optimal basecalling adjacent to the primer and for sequencing short PCR product templates with rapid electrophoresis run modules • Improve the quality of your results for a wide range of sequencing applications • Sequence challenging templates and sequences more successfully • Get longer higher quality reads with more uniform peak heights and optimal signal balance • Enhance your productivity and reduce costs Tackle Tough Sequences With the BigDye Terminator v1 1 Cycle Sequencing Kit you get a robust highly flexible chemistry for a wide range of applications including de novo sequencing and resequencing Get More Uniform Peak Heights Improved AccuracyWith BigDye Terminator v1 1 kit s superior chemistry you generate data with uniform peak heights and optimized signal balance which results in longer higher quality reads You also get more accurate base assignments for heterozygote and mutation detection Easily Integrate into Your WorkflowBigDye Terminator v 1 1 kit s chemistry requires no new software or instrument recalibration You can easily integrate either kit into your current workflow with minimal changes to your protocols For Research Use Only Not for use in diagnostics procedures
    https://www.bioz.com/result/bigdye terminator cycle sequencing kit/product/Thermo Fisher
    Average 99 stars, based on 292 article reviews
    Price from $9.99 to $1999.99
    bigdye terminator cycle sequencing kit - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Sequencing:

    Article Title: Efficient gene targeting by TAL effector nucleases coinjected with exonucleases in zygotes
    Article Snippet: .. Sequencing was performed using the BigDye Terminator Cycle Sequencing Kit and an ABI PRISM 3130 Genetic Analyzer (Life Technologies). .. Rat embryo culture Pronuclear stage embryos were collected from DA/Slc females at 7 weeks of age that had undergone super-ovulation by injection with PMSG (Serotropin, Aska Pharmaceutical Co., Tokyo, Japan) and hCG (Gonatropin, Aska Pharmaceutical Co.).

    Article Title: A frequent variant in the Japanese population determines quasi-Mendelian inheritance of rare retinal ciliopathy
    Article Snippet: .. PCR products displaying abnormal size profiles were purified (ExoSAP-IT, USB, Cleveland Ohio) and a sequencing reaction was performed in a total volume of 5 μl using 1 μl primer 3.3 µM, 0.5 μl BigDye Terminator v1.1, and 1 μl of the provided Buffer (Applied Biosystems, Foster City, California) Big Dye terminator cycle sequencing kit on an ABI 3130xl Genetic Analyzer (Applied Biosystems). ..

    Article Title: The Association of SERPINE2 Gene with COPD in a Chinese Han Population
    Article Snippet: .. Sequencing was performed using the BigDye Terminator v1.1 Cycle Sequencing kit (Applied Biosystems), and analyzed on an ABI PRISM 3100 DNA sequencer (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: Minimal residual disease detection in Tunisian B-acute lymphoblastic leukemia based on immunoglobulin gene rearrangements
    Article Snippet: .. Sequencing analysis Fluorescent sequencing was performed using the Big Dye-terminator cycle sequencing kit and the ABI Prism 3130xl Genetic Analyzer (Applied Biosystems). .. Sequences were analyzed with sequencing analysis 5.3 software (Applied Biosystems), identified using V-quest IMGT ( http://www.imgt.org/IMGT_vquest/vquest?livret=0 & Option=humanIg ) and confirmed with Ig-BLAST sequence similarity searching tool ( http://www.ncbi.nlm.nih.gov/igblast/ ).

    Article Title: Microcystic Stromal Tumor of Testicle: First Case Report and Literature Review
    Article Snippet: .. Bidirectional Sanger sequencing of all PCR products was performed subsequently on a 3500 Genetic Analyzer (Life Technologies, Carlsbad, CA) using the BigDye Terminator v1.1 Cycle Sequencing Kit (Life Technologies), according to standard protocols. ..

    Article Title: Comprehensive and Rapid Genotyping of Mutations and Haplotypes in Congenital Bilateral Absence of the Vas Deferens and Other Cystic Fibrosis Transmembrane Conductance Regulator-Related Disorders
    Article Snippet: .. Samples showing abnormal profiles were reamplified from genomic DNA and directly sequenced with the BigDye Terminator v1.1 cycle sequencing kit from Applied Biosystems (Warrington, UK). .. Samples found with only one or no CFTR disease-causing mutation were further investigated for large rearrangements such as large deletions by a semiquantitative fluorescent PCR assay previously developed in our laboratory that uses three multiplex PCRs covering the entire gene.

    Article Title: Distribution of MEFV gene mutations and R202Q polymorphism in the Serbian population and their influence on oxidative stress and clinical manifestations of inflammation
    Article Snippet: .. Thereafter, sequencing was carried out using the Big Dye Terminator cycle sequencing kit and ABI PRISM 310 automated sequencer (PE Applied Biosystems, Norwalk, USA). ..

    Article Title: An unnatural base pair system for efficient PCR amplification and functionalization of DNA molecules
    Article Snippet: .. DNA sequencing The cycle sequencing reaction (20 μl) was performed with the Cycle Sequencing Mix (8 μl) from the BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems), containing ∼0.3 pmol of the template and 4 pmol of the sequencing primer (20-mer), in the presence of 40 pmol or 1 nmol of 4-propynyl-1-(β-D-ribofuranosyl)pyrrole-2-carbaldehyde 5′-triphosphate (d Pa′ TP) (40 pmol for DNA fragments containing one Ds base and 1 nmol for DNAs with two Ds bases) or 1 nmol of dd Pa′ TP. .. After 25 cycles of PCR (96°C, 10 s; 50°C, 5 s; 60°C, 4 min), the residual dye terminators were removed from the reaction with CENTRI-SEP columns (Princeton Separations), and the solutions were dried.

    Polymerase Chain Reaction:

    Article Title: A frequent variant in the Japanese population determines quasi-Mendelian inheritance of rare retinal ciliopathy
    Article Snippet: .. PCR products displaying abnormal size profiles were purified (ExoSAP-IT, USB, Cleveland Ohio) and a sequencing reaction was performed in a total volume of 5 μl using 1 μl primer 3.3 µM, 0.5 μl BigDye Terminator v1.1, and 1 μl of the provided Buffer (Applied Biosystems, Foster City, California) Big Dye terminator cycle sequencing kit on an ABI 3130xl Genetic Analyzer (Applied Biosystems). ..

    Article Title: Microcystic Stromal Tumor of Testicle: First Case Report and Literature Review
    Article Snippet: .. Bidirectional Sanger sequencing of all PCR products was performed subsequently on a 3500 Genetic Analyzer (Life Technologies, Carlsbad, CA) using the BigDye Terminator v1.1 Cycle Sequencing Kit (Life Technologies), according to standard protocols. ..

    DNA Sequencing:

    Article Title: An unnatural base pair system for efficient PCR amplification and functionalization of DNA molecules
    Article Snippet: .. DNA sequencing The cycle sequencing reaction (20 μl) was performed with the Cycle Sequencing Mix (8 μl) from the BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems), containing ∼0.3 pmol of the template and 4 pmol of the sequencing primer (20-mer), in the presence of 40 pmol or 1 nmol of 4-propynyl-1-(β-D-ribofuranosyl)pyrrole-2-carbaldehyde 5′-triphosphate (d Pa′ TP) (40 pmol for DNA fragments containing one Ds base and 1 nmol for DNAs with two Ds bases) or 1 nmol of dd Pa′ TP. .. After 25 cycles of PCR (96°C, 10 s; 50°C, 5 s; 60°C, 4 min), the residual dye terminators were removed from the reaction with CENTRI-SEP columns (Princeton Separations), and the solutions were dried.

    Purification:

    Article Title: A frequent variant in the Japanese population determines quasi-Mendelian inheritance of rare retinal ciliopathy
    Article Snippet: .. PCR products displaying abnormal size profiles were purified (ExoSAP-IT, USB, Cleveland Ohio) and a sequencing reaction was performed in a total volume of 5 μl using 1 μl primer 3.3 µM, 0.5 μl BigDye Terminator v1.1, and 1 μl of the provided Buffer (Applied Biosystems, Foster City, California) Big Dye terminator cycle sequencing kit on an ABI 3130xl Genetic Analyzer (Applied Biosystems). ..

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  • 99
    Thermo Fisher bigdye terminater v3 1 cycle sequencing kit
    Bigdye Terminater V3 1 Cycle Sequencing Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bigdye terminater v3 1 cycle sequencing kit/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bigdye terminater v3 1 cycle sequencing kit - by Bioz Stars, 2020-07
    99/100 stars
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