bigdye terminator cycle sequencing kit  (Thermo Fisher)


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    Thermo Fisher bigdye terminator cycle sequencing kit
    Bigdye Terminator Cycle Sequencing Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bigdye terminator cycle sequencing kit/product/Thermo Fisher
    Average 99 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    bigdye terminator cycle sequencing kit - by Bioz Stars, 2020-01
    99/100 stars

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    Related Articles

    Centrifugation:

    Article Title: A purely quantitative form of partial recessive IFN-γR2 deficiency caused by mutations of the initiation or second codon
    Article Snippet: .. The PCR products were purified by centrifugation through Sephadex G-50 Superfine resin (GE Healthcare) and sequenced with the BigDye Terminator Cycle Sequencing kit (Applied Biosystems). .. Sequencing products were purified by centrifugation through Sephadex G-50 Superfine resin, and sequences were analyzed with an ABI Prism 3700 apparatus (Applied Biosystems).

    Amplification:

    Article Title: Significant association of rare variant p.Gly8Ser in cardiac sodium channel β4-subunit SCN4B with atrial fibrillation
    Article Snippet: All exons and exon–intron boundaries were amplified using polymerase chain reactions using the primers listed in . .. DNA sequencing analysis was performed using a BigDye Terminator Cycle Sequencing Kit by Shanghai Sangni Biotechnology (Applied Biosystems, Foster City, CA) as described ( ; ).

    Article Title: The genus Massalongia (lichenised ascomycetae) in the Southern Hemisphere
    Article Snippet: Paragraph title: DNA extraction, Amplification and Sequencing ... Direct sequencing of PCR products was run with the PCR primers using a BigDye Terminator Cycle Sequencing kit (Applied Biosystems) on an ABI Prism 3700XL DNA analyser (Applied Biosystems) at the DNA Sequencing Facility (UiB), Norway.

    Article Title: CRISPR-Cas3 induces broad and unidirectional genome editing in human cells
    Article Snippet: The targeted regions were amplified using Quick Taq HS DyeMix (Toyobo), KOD FX Neo (Toyobo) or Gflex (Takara Bio) and electrophoresed on an agarose gel or Agilent 2200 Tape Station (Agilent Technologies). .. Sequencing analysis was performed using the BigDye Terminator Cycle Sequencing Kit and an ABI PRISM 3130 Genetic Analyzer (Thermo Fisher Scientific).

    Article Title: Five major sequence variants and copy number variants in the EYS gene account for one-third of Japanese patients with autosomal recessive and simplex retinitis pigmentosa
    Article Snippet: .. Two hundred nanograms of genomic DNA were amplified with Taq polymerase (TaKaRa PrimeSTAR; Takara Bio Inc., Shiga, Japan), and a sequence variant analysis was performed with direct sequencing of the purified PCR products (BigDye Terminator Cycle Sequencing Kit; Applied Biosystems, Foster City, CA). .. Sequencing reaction products were run on an automated capillary sequencer (3130xl Genetic Analyzer; Applied Biosystems).

    Article Title: The co-occurrence of rare non-ocular phenotypes in patients with inherited retinal degenerations
    Article Snippet: Testing for specific mutations and verification of mutations identified with WES were performed by PCR amplification with specifically designed primers. .. This was followed by direct sequencing with the BigDye Terminator Cycle Sequencing Kit on an ABI 3130xl Genetic Analyzer (PE Applied Biosystems).

    Article Title: A novel sequestrate species from Mexico: Aroramycesguanajuatensis sp. nov. ( Hysterangiaceae, Hysterangiales)
    Article Snippet: Paragraph title: DNA extraction, amplification, sequencing and phylogenetic analyses ... Finally, the products were purified using the ExoSap Kit (Affymetrix, USA) according to the manufacturer’s instructions and were prepared for the sequencing reaction using the BigDye Terminator Cycle Sequencing Kit v. 3.1 (Applied BioSystems).

    Article Title: Newborn screening for cerebrotendinous xanthomatosis is the solution for early identification and treatment
    Article Snippet: For Moroccan Jews, all three known pathogenic genetic variants were tested; the c.1016 C > T (pThr339Met) variant by amplification of a 382 bp fragment from exon 5 in CYP27A1 using sense primer (5′-TGCTTTCACAGGGAAGAAGC-3′) and antisense primer (5′-ACAAAAGGACCATCCCCTGT-3′). .. Sequences were determined using the BigDye Terminator cycle sequencing kit (Applied Biosystems, Foster City, CA) and analyzed using the ABI PRISM 3500 XL genetic analyzer (Applied Biosystems).

    Article Title: Necrotic and Cytolytic Activity on Grapevine Leaves Produced by Nep1-Like Proteins of Diplodia seriata
    Article Snippet: Paragraph title: PCR Amplification and Sequencing of DserNEP Genes ... The program consisted of an initial step of 2 min at 95°C, followed by 35 cycles of denaturation at 95°C for 20 s, annealing at 60°C for 15 s, and elongation at 72°C for 30 s. A final extension was performed at 72°C for 3 min. DNA was sequenced by the dideoxynucleotide chain termination method using a BigDye Terminator cycle sequencing kit (Applied Biosystems).

    Article Title: Identification of rare variants in cardiac sodium channel β4‑subunit gene SCN4B associated with ventricular tachycardia
    Article Snippet: All exons and exon–intron boundaries were amplified using polymerase chain reactions (PCR) and subjected to high-resolution melt analysis (HRM) as described ( , ). .. DNA sequencing was performed using BigDye® Terminator Cycle Sequencing Kit (Applied Biosystems) by Shanghai Sangni Company.

    TA Cloning:

    Article Title: CRISPR-Cas3 induces broad and unidirectional genome editing in human cells
    Article Snippet: For TA cloning, the PCR products were purified using a DNA Clean Up Kit and subcloned into a pCR4Blunt-TOPO plasmid vector (Thermo Fisher Scientific) according to the manufacturer’s protocol. .. Sequencing analysis was performed using the BigDye Terminator Cycle Sequencing Kit and an ABI PRISM 3130 Genetic Analyzer (Thermo Fisher Scientific).

    Real-time Polymerase Chain Reaction:

    Article Title: Investigation of urban birds as source of β-lactamase-producing Gram-negative bacteria in Marseille city, France
    Article Snippet: On overall, by qPCR and standard PCR we investigated genes encoding for extend-spectrum-β-lactamases (bla SHV , bla VEB , bla GES , bla PER ), carbapenemases (VIM, NDM, OXA-23, OXA-24, OXA-48, OXA-58), and mobile colistin resistance genes (mcr -1 to mcr -5 ) using primers and probes previously reported [ ]. .. Sequencing was performed using the BigDye Terminator Cycle Sequencing kit and an automated fluorescent DNA sequencer ABI 3730xl (Applied Biosystems, Foster City, CA, USA) in accordance with the manufacturer’s instructions.

    Article Title: Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens, et al. Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens
    Article Snippet: PCR was performed in a total volume of 20 μL (10 μL PCR reaction mixture, 5 μL probe mixture, and 5 μL DNA) using the 7500 Fast Real‐Time PCR System. .. After detection of PCR products by gel electrophoresis and purification, sequencing was performed on an automated ABI Prism 3730 instrument using a BigDye terminator cycle sequencing kit (Applied Biosystems, Foster City, CA, USA).

    Microarray:

    Article Title: The co-occurrence of rare non-ocular phenotypes in patients with inherited retinal degenerations
    Article Snippet: Chromosomal microarray analysis (CMA) in family 2 was performed using the CytoScanTM 750K Chip Kit (PE Applied Biosystems, Foster City, CA). .. This was followed by direct sequencing with the BigDye Terminator Cycle Sequencing Kit on an ABI 3130xl Genetic Analyzer (PE Applied Biosystems).

    Expressing:

    Article Title: CRISPR-Cas3 induces broad and unidirectional genome editing in human cells
    Article Snippet: Detection of large deletion mutations in human cells At 24 h after seeding 5.0 × 104 293T cells in each well of a 24-well plate, 250 ng each of Cas3, Cas5, Cas6, Cas7, Cas8, Cas11, and crRNA expression vectors were transfected into 293T cells as described above. .. Sequencing analysis was performed using the BigDye Terminator Cycle Sequencing Kit and an ABI PRISM 3130 Genetic Analyzer (Thermo Fisher Scientific).

    Transfection:

    Article Title: CRISPR-Cas3 induces broad and unidirectional genome editing in human cells
    Article Snippet: Two days after the transfection, whole DNA was extracted from collected cells using the Tissue XS Kit (Takara Bio) according to the manufacturer’s protocol. .. Sequencing analysis was performed using the BigDye Terminator Cycle Sequencing Kit and an ABI PRISM 3130 Genetic Analyzer (Thermo Fisher Scientific).

    Cell Culture:

    Article Title: Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens, et al. Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens
    Article Snippet: After detection of PCR products by gel electrophoresis and purification, sequencing was performed on an automated ABI Prism 3730 instrument using a BigDye terminator cycle sequencing kit (Applied Biosystems, Foster City, CA, USA). .. Analytical sensitivity was determined by directly comparing dilutions of six type strains in cell culture and in the DiaPlex assay.

    Generated:

    Article Title: The genus Massalongia (lichenised ascomycetae) in the Southern Hemisphere
    Article Snippet: Direct sequencing of PCR products was run with the PCR primers using a BigDye Terminator Cycle Sequencing kit (Applied Biosystems) on an ABI Prism 3700XL DNA analyser (Applied Biosystems) at the DNA Sequencing Facility (UiB), Norway. .. Newly generated sequences with GenBank accession numbers are listed in Tables , , together with sequences downloaded from GenBank.

    DNA Sequencing:

    Article Title: Significant association of rare variant p.Gly8Ser in cardiac sodium channel β4-subunit SCN4B with atrial fibrillation
    Article Snippet: .. DNA sequencing analysis was performed using a BigDye Terminator Cycle Sequencing Kit by Shanghai Sangni Biotechnology (Applied Biosystems, Foster City, CA) as described ( ; ). .. Sequencing data were directly viewed using Sequencing Analysis (version 5.1.1, Applied Biosystems) to identify potential variants.

    Article Title: An enhancer cluster controls gene activity and topology of the SCN5A-SCN10A locus in vivo
    Article Snippet: .. Sanger sequencing DNA sequencing was performed with BigDye Terminator Cycle Sequencing Kit (Applied Biosystems). .. The BigDye Cycle Sequence sequencing reaction utilized the following cycling parameters: initial denaturation at 96 °C (10 s), denaturation at 96 °C (30 s), with an intervening 40 cycles of prime annealing at 50 °C (15 s), elongation 60 °C (4 min).

    Article Title: The genus Massalongia (lichenised ascomycetae) in the Southern Hemisphere
    Article Snippet: .. Direct sequencing of PCR products was run with the PCR primers using a BigDye Terminator Cycle Sequencing kit (Applied Biosystems) on an ABI Prism 3700XL DNA analyser (Applied Biosystems) at the DNA Sequencing Facility (UiB), Norway. ..

    Article Title: High‐Resolution Melting Analysis as a Developed Method for Genotyping the PD Susceptibility Loci in LRRK2 Gene
    Article Snippet: .. The above PCR products from R1628P locus were purified, and then direct DNA sequencing was performed using the BigDye Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, CA). .. Because no homozygous CC in R1628P locus was previously detected, we constructed C and G alleles on the R1628P locus by TA‐clone to provide a distinguishable parameter between different genotyping methods.

    Article Title: Identification of rare variants in cardiac sodium channel β4‑subunit gene SCN4B associated with ventricular tachycardia
    Article Snippet: .. DNA sequencing was performed using BigDye® Terminator Cycle Sequencing Kit (Applied Biosystems) by Shanghai Sangni Company. .. Sequencing data were directly viewed using sequencing analysis 5.1.1 (Applied Biosystems) to identify potential variants.

    Sequencing:

    Article Title: Investigation of urban birds as source of β-lactamase-producing Gram-negative bacteria in Marseille city, France
    Article Snippet: .. Sequencing was performed using the BigDye Terminator Cycle Sequencing kit and an automated fluorescent DNA sequencer ABI 3730xl (Applied Biosystems, Foster City, CA, USA) in accordance with the manufacturer’s instructions. ..

    Article Title: Significant association of rare variant p.Gly8Ser in cardiac sodium channel β4-subunit SCN4B with atrial fibrillation
    Article Snippet: .. DNA sequencing analysis was performed using a BigDye Terminator Cycle Sequencing Kit by Shanghai Sangni Biotechnology (Applied Biosystems, Foster City, CA) as described ( ; ). .. Sequencing data were directly viewed using Sequencing Analysis (version 5.1.1, Applied Biosystems) to identify potential variants.

    Article Title: An enhancer cluster controls gene activity and topology of the SCN5A-SCN10A locus in vivo
    Article Snippet: .. Sanger sequencing DNA sequencing was performed with BigDye Terminator Cycle Sequencing Kit (Applied Biosystems). .. The BigDye Cycle Sequence sequencing reaction utilized the following cycling parameters: initial denaturation at 96 °C (10 s), denaturation at 96 °C (30 s), with an intervening 40 cycles of prime annealing at 50 °C (15 s), elongation 60 °C (4 min).

    Article Title: The genus Massalongia (lichenised ascomycetae) in the Southern Hemisphere
    Article Snippet: .. Direct sequencing of PCR products was run with the PCR primers using a BigDye Terminator Cycle Sequencing kit (Applied Biosystems) on an ABI Prism 3700XL DNA analyser (Applied Biosystems) at the DNA Sequencing Facility (UiB), Norway. ..

    Article Title: CRISPR-Cas3 induces broad and unidirectional genome editing in human cells
    Article Snippet: .. Sequencing analysis was performed using the BigDye Terminator Cycle Sequencing Kit and an ABI PRISM 3130 Genetic Analyzer (Thermo Fisher Scientific). .. To detect the ratio of variant mutations, DNA libraries of the PCR amplicons were prepared with a Nextera XT DNA Library Prep Kit or TruSeq Nano DNA Library Prep Kit (Illumina), and amplicon sequencing was performed by using MiSeq (2 × 150 bp) according to the standard procedure at Macrogen.

    Article Title: Five major sequence variants and copy number variants in the EYS gene account for one-third of Japanese patients with autosomal recessive and simplex retinitis pigmentosa
    Article Snippet: .. Two hundred nanograms of genomic DNA were amplified with Taq polymerase (TaKaRa PrimeSTAR; Takara Bio Inc., Shiga, Japan), and a sequence variant analysis was performed with direct sequencing of the purified PCR products (BigDye Terminator Cycle Sequencing Kit; Applied Biosystems, Foster City, CA). .. Sequencing reaction products were run on an automated capillary sequencer (3130xl Genetic Analyzer; Applied Biosystems).

    Article Title: The co-occurrence of rare non-ocular phenotypes in patients with inherited retinal degenerations
    Article Snippet: .. This was followed by direct sequencing with the BigDye Terminator Cycle Sequencing Kit on an ABI 3130xl Genetic Analyzer (PE Applied Biosystems). .. The primer sequences used for PCR amplification and Sanger sequencing are listed in Appendix 1.

    Article Title: High‐Resolution Melting Analysis as a Developed Method for Genotyping the PD Susceptibility Loci in LRRK2 Gene
    Article Snippet: .. The above PCR products from R1628P locus were purified, and then direct DNA sequencing was performed using the BigDye Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, CA). .. Because no homozygous CC in R1628P locus was previously detected, we constructed C and G alleles on the R1628P locus by TA‐clone to provide a distinguishable parameter between different genotyping methods.

    Article Title: Global Assessment of Mycobacterium avium subsp. hominissuis Genetic Requirement for Growth and Virulence
    Article Snippet: .. Sanger sequencing was performed using genomic DNA (gDNA) as the template prepared with BigDye Terminator Cycle Sequencing kit v.1.1 (Thermo Fisher); sequencing was conducted with 60 cycles of PCR, with 1 cycle consisting of 30 s at 95°C, 30 s at 52°C, and 4 min at 60°C, and primer KanSeq2 (CTTCCTCGTGCTTTACGG) reading directly into the gDNA. .. Samples were purified using BigDye XTerminator kit (Applied Biosystems) and sequenced on an ABI130xl.

    Article Title: A novel sequestrate species from Mexico: Aroramycesguanajuatensis sp. nov. ( Hysterangiaceae, Hysterangiales)
    Article Snippet: .. Finally, the products were purified using the ExoSap Kit (Affymetrix, USA) according to the manufacturer’s instructions and were prepared for the sequencing reaction using the BigDye Terminator Cycle Sequencing Kit v. 3.1 (Applied BioSystems). .. Sequencing was carried out in a genetic analyzer (Model 3130XL, Applied BioSystems, USA) at the Biology Institute of the National Autonomous University of Mexico ( UNAM ).

    Article Title: A purely quantitative form of partial recessive IFN-γR2 deficiency caused by mutations of the initiation or second codon
    Article Snippet: .. The PCR products were purified by centrifugation through Sephadex G-50 Superfine resin (GE Healthcare) and sequenced with the BigDye Terminator Cycle Sequencing kit (Applied Biosystems). .. Sequencing products were purified by centrifugation through Sephadex G-50 Superfine resin, and sequences were analyzed with an ABI Prism 3700 apparatus (Applied Biosystems).

    Article Title: Newborn screening for cerebrotendinous xanthomatosis is the solution for early identification and treatment
    Article Snippet: .. Sequences were determined using the BigDye Terminator cycle sequencing kit (Applied Biosystems, Foster City, CA) and analyzed using the ABI PRISM 3500 XL genetic analyzer (Applied Biosystems). .. Quality control (QC) and calibrator DBS samples were generated using working dilutions of 5β-cholestane-25-tetrol-glucuronide (from stock solution stored in 85:15 acetonitrile:water) and 7α12αC4 in methanol.

    Article Title: Necrotic and Cytolytic Activity on Grapevine Leaves Produced by Nep1-Like Proteins of Diplodia seriata
    Article Snippet: .. The program consisted of an initial step of 2 min at 95°C, followed by 35 cycles of denaturation at 95°C for 20 s, annealing at 60°C for 15 s, and elongation at 72°C for 30 s. A final extension was performed at 72°C for 3 min. DNA was sequenced by the dideoxynucleotide chain termination method using a BigDye Terminator cycle sequencing kit (Applied Biosystems). ..

    Article Title: Identification of rare variants in cardiac sodium channel β4‑subunit gene SCN4B associated with ventricular tachycardia
    Article Snippet: .. DNA sequencing was performed using BigDye® Terminator Cycle Sequencing Kit (Applied Biosystems) by Shanghai Sangni Company. .. Sequencing data were directly viewed using sequencing analysis 5.1.1 (Applied Biosystems) to identify potential variants.

    Article Title: Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens, et al. Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens
    Article Snippet: .. After detection of PCR products by gel electrophoresis and purification, sequencing was performed on an automated ABI Prism 3730 instrument using a BigDye terminator cycle sequencing kit (Applied Biosystems, Foster City, CA, USA). .. Analytical sensitivity was determined by directly comparing dilutions of six type strains in cell culture and in the DiaPlex assay.

    DNA Extraction:

    Article Title: The genus Massalongia (lichenised ascomycetae) in the Southern Hemisphere
    Article Snippet: Paragraph title: DNA extraction, Amplification and Sequencing ... Direct sequencing of PCR products was run with the PCR primers using a BigDye Terminator Cycle Sequencing kit (Applied Biosystems) on an ABI Prism 3700XL DNA analyser (Applied Biosystems) at the DNA Sequencing Facility (UiB), Norway.

    Article Title: Five major sequence variants and copy number variants in the EYS gene account for one-third of Japanese patients with autosomal recessive and simplex retinitis pigmentosa
    Article Snippet: Sequencing analysis Genomic DNA was isolated from venous blood using a DNA purification kit (Puregene; Gentra Systems, Minneapolis, MN) for the subjects of NRCD and a DNA extraction kit (Quick-Gene-610L; Fujifilm, Tokyo, Japan) for the patients of Kyoto University. .. Two hundred nanograms of genomic DNA were amplified with Taq polymerase (TaKaRa PrimeSTAR; Takara Bio Inc., Shiga, Japan), and a sequence variant analysis was performed with direct sequencing of the purified PCR products (BigDye Terminator Cycle Sequencing Kit; Applied Biosystems, Foster City, CA).

    Article Title: Global Assessment of Mycobacterium avium subsp. hominissuis Genetic Requirement for Growth and Virulence
    Article Snippet: Total DNA was isolated using Masterpure Complete DNA Isolation kit (Epicentre). .. Sanger sequencing was performed using genomic DNA (gDNA) as the template prepared with BigDye Terminator Cycle Sequencing kit v.1.1 (Thermo Fisher); sequencing was conducted with 60 cycles of PCR, with 1 cycle consisting of 30 s at 95°C, 30 s at 52°C, and 4 min at 60°C, and primer KanSeq2 (CTTCCTCGTGCTTTACGG) reading directly into the gDNA.

    Article Title: A novel sequestrate species from Mexico: Aroramycesguanajuatensis sp. nov. ( Hysterangiaceae, Hysterangiales)
    Article Snippet: Paragraph title: DNA extraction, amplification, sequencing and phylogenetic analyses ... Finally, the products were purified using the ExoSap Kit (Affymetrix, USA) according to the manufacturer’s instructions and were prepared for the sequencing reaction using the BigDye Terminator Cycle Sequencing Kit v. 3.1 (Applied BioSystems).

    Article Title: Newborn screening for cerebrotendinous xanthomatosis is the solution for early identification and treatment
    Article Snippet: Paragraph title: DNA extraction from DBSs and genetic analysis ... Sequences were determined using the BigDye Terminator cycle sequencing kit (Applied Biosystems, Foster City, CA) and analyzed using the ABI PRISM 3500 XL genetic analyzer (Applied Biosystems).

    Nucleic Acid Electrophoresis:

    Article Title: Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens, et al. Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens
    Article Snippet: .. After detection of PCR products by gel electrophoresis and purification, sequencing was performed on an automated ABI Prism 3730 instrument using a BigDye terminator cycle sequencing kit (Applied Biosystems, Foster City, CA, USA). .. Analytical sensitivity was determined by directly comparing dilutions of six type strains in cell culture and in the DiaPlex assay.

    Mutagenesis:

    Article Title: CRISPR-Cas3 induces broad and unidirectional genome editing in human cells
    Article Snippet: To detect small indel mutations in the PCR products, T7 endonuclease I (New England Biolabs) or the SURVEYOR Mutation Detection Kit (Integrated DNA Technologies) was used in accordance with the manufacturer’s protocol. .. Sequencing analysis was performed using the BigDye Terminator Cycle Sequencing Kit and an ABI PRISM 3130 Genetic Analyzer (Thermo Fisher Scientific).

    Isolation:

    Article Title: Five major sequence variants and copy number variants in the EYS gene account for one-third of Japanese patients with autosomal recessive and simplex retinitis pigmentosa
    Article Snippet: Genomic DNA of family members was isolated from their saliva using a DNA self-collection kit (Oragene® -DNA OG-500; DNA Genotek, Ottawa, Canada). .. Two hundred nanograms of genomic DNA were amplified with Taq polymerase (TaKaRa PrimeSTAR; Takara Bio Inc., Shiga, Japan), and a sequence variant analysis was performed with direct sequencing of the purified PCR products (BigDye Terminator Cycle Sequencing Kit; Applied Biosystems, Foster City, CA).

    Article Title: Global Assessment of Mycobacterium avium subsp. hominissuis Genetic Requirement for Growth and Virulence
    Article Snippet: Total DNA was isolated using Masterpure Complete DNA Isolation kit (Epicentre). .. Sanger sequencing was performed using genomic DNA (gDNA) as the template prepared with BigDye Terminator Cycle Sequencing kit v.1.1 (Thermo Fisher); sequencing was conducted with 60 cycles of PCR, with 1 cycle consisting of 30 s at 95°C, 30 s at 52°C, and 4 min at 60°C, and primer KanSeq2 (CTTCCTCGTGCTTTACGG) reading directly into the gDNA.

    Size-exclusion Chromatography:

    Article Title: The genus Massalongia (lichenised ascomycetae) in the Southern Hemisphere
    Article Snippet: PCR reactions were performed on a C1000 Touch thermal cycler (Bio-Rad Laboratories), with the following temperatures; initial denaturation at 94 °C for 4 min, followed by a 62–56 °C touchdown annealing for the first 6 cycles, ending with 30 cycles at 56 °C for 30 sec, polymerisation at 72 °C for 1 min 45 sec and a final elongation at 72 °C for 10 min. .. Direct sequencing of PCR products was run with the PCR primers using a BigDye Terminator Cycle Sequencing kit (Applied Biosystems) on an ABI Prism 3700XL DNA analyser (Applied Biosystems) at the DNA Sequencing Facility (UiB), Norway.

    Purification:

    Article Title: Investigation of urban birds as source of β-lactamase-producing Gram-negative bacteria in Marseille city, France
    Article Snippet: All positive PCR products for genes encoding for ESBL that conferred resistance to 3rd generation cephalosporins were purified using the QIAquick PCR purification kit in concordance with the manufacturer’s instructions (Qiagen, Hilden, Germany). .. Sequencing was performed using the BigDye Terminator Cycle Sequencing kit and an automated fluorescent DNA sequencer ABI 3730xl (Applied Biosystems, Foster City, CA, USA) in accordance with the manufacturer’s instructions.

    Article Title: CRISPR-Cas3 induces broad and unidirectional genome editing in human cells
    Article Snippet: For TA cloning, the PCR products were purified using a DNA Clean Up Kit and subcloned into a pCR4Blunt-TOPO plasmid vector (Thermo Fisher Scientific) according to the manufacturer’s protocol. .. Sequencing analysis was performed using the BigDye Terminator Cycle Sequencing Kit and an ABI PRISM 3130 Genetic Analyzer (Thermo Fisher Scientific).

    Article Title: Five major sequence variants and copy number variants in the EYS gene account for one-third of Japanese patients with autosomal recessive and simplex retinitis pigmentosa
    Article Snippet: .. Two hundred nanograms of genomic DNA were amplified with Taq polymerase (TaKaRa PrimeSTAR; Takara Bio Inc., Shiga, Japan), and a sequence variant analysis was performed with direct sequencing of the purified PCR products (BigDye Terminator Cycle Sequencing Kit; Applied Biosystems, Foster City, CA). .. Sequencing reaction products were run on an automated capillary sequencer (3130xl Genetic Analyzer; Applied Biosystems).

    Article Title: High‐Resolution Melting Analysis as a Developed Method for Genotyping the PD Susceptibility Loci in LRRK2 Gene
    Article Snippet: .. The above PCR products from R1628P locus were purified, and then direct DNA sequencing was performed using the BigDye Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, CA). .. Because no homozygous CC in R1628P locus was previously detected, we constructed C and G alleles on the R1628P locus by TA‐clone to provide a distinguishable parameter between different genotyping methods.

    Article Title: Global Assessment of Mycobacterium avium subsp. hominissuis Genetic Requirement for Growth and Virulence
    Article Snippet: Sanger sequencing was performed using genomic DNA (gDNA) as the template prepared with BigDye Terminator Cycle Sequencing kit v.1.1 (Thermo Fisher); sequencing was conducted with 60 cycles of PCR, with 1 cycle consisting of 30 s at 95°C, 30 s at 52°C, and 4 min at 60°C, and primer KanSeq2 (CTTCCTCGTGCTTTACGG) reading directly into the gDNA. .. Samples were purified using BigDye XTerminator kit (Applied Biosystems) and sequenced on an ABI130xl.

    Article Title: A novel sequestrate species from Mexico: Aroramycesguanajuatensis sp. nov. ( Hysterangiaceae, Hysterangiales)
    Article Snippet: .. Finally, the products were purified using the ExoSap Kit (Affymetrix, USA) according to the manufacturer’s instructions and were prepared for the sequencing reaction using the BigDye Terminator Cycle Sequencing Kit v. 3.1 (Applied BioSystems). .. Sequencing was carried out in a genetic analyzer (Model 3130XL, Applied BioSystems, USA) at the Biology Institute of the National Autonomous University of Mexico ( UNAM ).

    Article Title: A purely quantitative form of partial recessive IFN-γR2 deficiency caused by mutations of the initiation or second codon
    Article Snippet: .. The PCR products were purified by centrifugation through Sephadex G-50 Superfine resin (GE Healthcare) and sequenced with the BigDye Terminator Cycle Sequencing kit (Applied Biosystems). .. Sequencing products were purified by centrifugation through Sephadex G-50 Superfine resin, and sequences were analyzed with an ABI Prism 3700 apparatus (Applied Biosystems).

    Article Title: Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens, et al. Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens
    Article Snippet: .. After detection of PCR products by gel electrophoresis and purification, sequencing was performed on an automated ABI Prism 3730 instrument using a BigDye terminator cycle sequencing kit (Applied Biosystems, Foster City, CA, USA). .. Analytical sensitivity was determined by directly comparing dilutions of six type strains in cell culture and in the DiaPlex assay.

    Polymerase Chain Reaction:

    Article Title: Investigation of urban birds as source of β-lactamase-producing Gram-negative bacteria in Marseille city, France
    Article Snippet: All positive PCR products for genes encoding for ESBL that conferred resistance to 3rd generation cephalosporins were purified using the QIAquick PCR purification kit in concordance with the manufacturer’s instructions (Qiagen, Hilden, Germany). .. Sequencing was performed using the BigDye Terminator Cycle Sequencing kit and an automated fluorescent DNA sequencer ABI 3730xl (Applied Biosystems, Foster City, CA, USA) in accordance with the manufacturer’s instructions.

    Article Title: The genus Massalongia (lichenised ascomycetae) in the Southern Hemisphere
    Article Snippet: .. Direct sequencing of PCR products was run with the PCR primers using a BigDye Terminator Cycle Sequencing kit (Applied Biosystems) on an ABI Prism 3700XL DNA analyser (Applied Biosystems) at the DNA Sequencing Facility (UiB), Norway. ..

    Article Title: CRISPR-Cas3 induces broad and unidirectional genome editing in human cells
    Article Snippet: For TA cloning, the PCR products were purified using a DNA Clean Up Kit and subcloned into a pCR4Blunt-TOPO plasmid vector (Thermo Fisher Scientific) according to the manufacturer’s protocol. .. Sequencing analysis was performed using the BigDye Terminator Cycle Sequencing Kit and an ABI PRISM 3130 Genetic Analyzer (Thermo Fisher Scientific).

    Article Title: Five major sequence variants and copy number variants in the EYS gene account for one-third of Japanese patients with autosomal recessive and simplex retinitis pigmentosa
    Article Snippet: .. Two hundred nanograms of genomic DNA were amplified with Taq polymerase (TaKaRa PrimeSTAR; Takara Bio Inc., Shiga, Japan), and a sequence variant analysis was performed with direct sequencing of the purified PCR products (BigDye Terminator Cycle Sequencing Kit; Applied Biosystems, Foster City, CA). .. Sequencing reaction products were run on an automated capillary sequencer (3130xl Genetic Analyzer; Applied Biosystems).

    Article Title: The co-occurrence of rare non-ocular phenotypes in patients with inherited retinal degenerations
    Article Snippet: PCR was performed on 50 ng of genomic DNA in a 25 µl reaction volume in the presence of 5X Readymix (LAROVA GmbH, Teltow, Germany) and 10 pmol of each forward and reverse primers. .. This was followed by direct sequencing with the BigDye Terminator Cycle Sequencing Kit on an ABI 3130xl Genetic Analyzer (PE Applied Biosystems).

    Article Title: High‐Resolution Melting Analysis as a Developed Method for Genotyping the PD Susceptibility Loci in LRRK2 Gene
    Article Snippet: .. The above PCR products from R1628P locus were purified, and then direct DNA sequencing was performed using the BigDye Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, CA). .. Because no homozygous CC in R1628P locus was previously detected, we constructed C and G alleles on the R1628P locus by TA‐clone to provide a distinguishable parameter between different genotyping methods.

    Article Title: Global Assessment of Mycobacterium avium subsp. hominissuis Genetic Requirement for Growth and Virulence
    Article Snippet: .. Sanger sequencing was performed using genomic DNA (gDNA) as the template prepared with BigDye Terminator Cycle Sequencing kit v.1.1 (Thermo Fisher); sequencing was conducted with 60 cycles of PCR, with 1 cycle consisting of 30 s at 95°C, 30 s at 52°C, and 4 min at 60°C, and primer KanSeq2 (CTTCCTCGTGCTTTACGG) reading directly into the gDNA. .. Samples were purified using BigDye XTerminator kit (Applied Biosystems) and sequenced on an ABI130xl.

    Article Title: A novel sequestrate species from Mexico: Aroramycesguanajuatensis sp. nov. ( Hysterangiaceae, Hysterangiales)
    Article Snippet: The PCR products were verified by agarose gel electrophoresis run for 1 h at 95 V cm-3 in 1.5% agarose and 1× TAE buffer (Tris Acetate-EDTA). .. Finally, the products were purified using the ExoSap Kit (Affymetrix, USA) according to the manufacturer’s instructions and were prepared for the sequencing reaction using the BigDye Terminator Cycle Sequencing Kit v. 3.1 (Applied BioSystems).

    Article Title: A purely quantitative form of partial recessive IFN-γR2 deficiency caused by mutations of the initiation or second codon
    Article Snippet: .. The PCR products were purified by centrifugation through Sephadex G-50 Superfine resin (GE Healthcare) and sequenced with the BigDye Terminator Cycle Sequencing kit (Applied Biosystems). .. Sequencing products were purified by centrifugation through Sephadex G-50 Superfine resin, and sequences were analyzed with an ABI Prism 3700 apparatus (Applied Biosystems).

    Article Title: Newborn screening for cerebrotendinous xanthomatosis is the solution for early identification and treatment
    Article Snippet: The PCR products were digested with HpyCH4 III restriction enzyme (New England Biolabs) and analyzed on 3.5% agarose gels. .. Sequences were determined using the BigDye Terminator cycle sequencing kit (Applied Biosystems, Foster City, CA) and analyzed using the ABI PRISM 3500 XL genetic analyzer (Applied Biosystems).

    Article Title: Necrotic and Cytolytic Activity on Grapevine Leaves Produced by Nep1-Like Proteins of Diplodia seriata
    Article Snippet: Paragraph title: PCR Amplification and Sequencing of DserNEP Genes ... The program consisted of an initial step of 2 min at 95°C, followed by 35 cycles of denaturation at 95°C for 20 s, annealing at 60°C for 15 s, and elongation at 72°C for 30 s. A final extension was performed at 72°C for 3 min. DNA was sequenced by the dideoxynucleotide chain termination method using a BigDye Terminator cycle sequencing kit (Applied Biosystems).

    Article Title: Identification of rare variants in cardiac sodium channel β4‑subunit gene SCN4B associated with ventricular tachycardia
    Article Snippet: The primers used to PCR and HRM analyses were designed using the primer-3 web site and listed in . .. DNA sequencing was performed using BigDye® Terminator Cycle Sequencing Kit (Applied Biosystems) by Shanghai Sangni Company.

    Article Title: Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens, et al. Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens
    Article Snippet: .. After detection of PCR products by gel electrophoresis and purification, sequencing was performed on an automated ABI Prism 3730 instrument using a BigDye terminator cycle sequencing kit (Applied Biosystems, Foster City, CA, USA). .. Analytical sensitivity was determined by directly comparing dilutions of six type strains in cell culture and in the DiaPlex assay.

    Chromatin Immunoprecipitation:

    Article Title: The co-occurrence of rare non-ocular phenotypes in patients with inherited retinal degenerations
    Article Snippet: Chromosomal microarray analysis (CMA) in family 2 was performed using the CytoScanTM 750K Chip Kit (PE Applied Biosystems, Foster City, CA). .. This was followed by direct sequencing with the BigDye Terminator Cycle Sequencing Kit on an ABI 3130xl Genetic Analyzer (PE Applied Biosystems).

    Plasmid Preparation:

    Article Title: CRISPR-Cas3 induces broad and unidirectional genome editing in human cells
    Article Snippet: For TA cloning, the PCR products were purified using a DNA Clean Up Kit and subcloned into a pCR4Blunt-TOPO plasmid vector (Thermo Fisher Scientific) according to the manufacturer’s protocol. .. Sequencing analysis was performed using the BigDye Terminator Cycle Sequencing Kit and an ABI PRISM 3130 Genetic Analyzer (Thermo Fisher Scientific).

    Software:

    Article Title: A purely quantitative form of partial recessive IFN-γR2 deficiency caused by mutations of the initiation or second codon
    Article Snippet: The PCR products were purified by centrifugation through Sephadex G-50 Superfine resin (GE Healthcare) and sequenced with the BigDye Terminator Cycle Sequencing kit (Applied Biosystems). .. The sequences obtained were then aligned with the genomic sequence of IFNGR2 ( ) with Genalys, version 2.0 β software.

    Agarose Gel Electrophoresis:

    Article Title: CRISPR-Cas3 induces broad and unidirectional genome editing in human cells
    Article Snippet: The targeted regions were amplified using Quick Taq HS DyeMix (Toyobo), KOD FX Neo (Toyobo) or Gflex (Takara Bio) and electrophoresed on an agarose gel or Agilent 2200 Tape Station (Agilent Technologies). .. Sequencing analysis was performed using the BigDye Terminator Cycle Sequencing Kit and an ABI PRISM 3130 Genetic Analyzer (Thermo Fisher Scientific).

    Article Title: A novel sequestrate species from Mexico: Aroramycesguanajuatensis sp. nov. ( Hysterangiaceae, Hysterangiales)
    Article Snippet: The PCR products were verified by agarose gel electrophoresis run for 1 h at 95 V cm-3 in 1.5% agarose and 1× TAE buffer (Tris Acetate-EDTA). .. Finally, the products were purified using the ExoSap Kit (Affymetrix, USA) according to the manufacturer’s instructions and were prepared for the sequencing reaction using the BigDye Terminator Cycle Sequencing Kit v. 3.1 (Applied BioSystems).

    DNA Purification:

    Article Title: Five major sequence variants and copy number variants in the EYS gene account for one-third of Japanese patients with autosomal recessive and simplex retinitis pigmentosa
    Article Snippet: Sequencing analysis Genomic DNA was isolated from venous blood using a DNA purification kit (Puregene; Gentra Systems, Minneapolis, MN) for the subjects of NRCD and a DNA extraction kit (Quick-Gene-610L; Fujifilm, Tokyo, Japan) for the patients of Kyoto University. .. Two hundred nanograms of genomic DNA were amplified with Taq polymerase (TaKaRa PrimeSTAR; Takara Bio Inc., Shiga, Japan), and a sequence variant analysis was performed with direct sequencing of the purified PCR products (BigDye Terminator Cycle Sequencing Kit; Applied Biosystems, Foster City, CA).

    Variant Assay:

    Article Title: Significant association of rare variant p.Gly8Ser in cardiac sodium channel β4-subunit SCN4B with atrial fibrillation
    Article Snippet: Paragraph title: 2.2 |. Mutational analysis of SCN4B and genotyping of variant p.Gly8Ser ... DNA sequencing analysis was performed using a BigDye Terminator Cycle Sequencing Kit by Shanghai Sangni Biotechnology (Applied Biosystems, Foster City, CA) as described ( ; ).

    Article Title: CRISPR-Cas3 induces broad and unidirectional genome editing in human cells
    Article Snippet: Sequencing analysis was performed using the BigDye Terminator Cycle Sequencing Kit and an ABI PRISM 3130 Genetic Analyzer (Thermo Fisher Scientific). .. To detect the ratio of variant mutations, DNA libraries of the PCR amplicons were prepared with a Nextera XT DNA Library Prep Kit or TruSeq Nano DNA Library Prep Kit (Illumina), and amplicon sequencing was performed by using MiSeq (2 × 150 bp) according to the standard procedure at Macrogen.

    Article Title: Five major sequence variants and copy number variants in the EYS gene account for one-third of Japanese patients with autosomal recessive and simplex retinitis pigmentosa
    Article Snippet: .. Two hundred nanograms of genomic DNA were amplified with Taq polymerase (TaKaRa PrimeSTAR; Takara Bio Inc., Shiga, Japan), and a sequence variant analysis was performed with direct sequencing of the purified PCR products (BigDye Terminator Cycle Sequencing Kit; Applied Biosystems, Foster City, CA). .. Sequencing reaction products were run on an automated capillary sequencer (3130xl Genetic Analyzer; Applied Biosystems).

    Article Title: Newborn screening for cerebrotendinous xanthomatosis is the solution for early identification and treatment
    Article Snippet: For Moroccan Jews, all three known pathogenic genetic variants were tested; the c.1016 C > T (pThr339Met) variant by amplification of a 382 bp fragment from exon 5 in CYP27A1 using sense primer (5′-TGCTTTCACAGGGAAGAAGC-3′) and antisense primer (5′-ACAAAAGGACCATCCCCTGT-3′). .. Sequences were determined using the BigDye Terminator cycle sequencing kit (Applied Biosystems, Foster City, CA) and analyzed using the ABI PRISM 3500 XL genetic analyzer (Applied Biosystems).

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    Thermo Fisher bigdye terminater v3 1 cycle sequencing kit
    Bigdye Terminater V3 1 Cycle Sequencing Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bigdye terminater v3 1 cycle sequencing kit/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
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    bigdye terminater v3 1 cycle sequencing kit - by Bioz Stars, 2020-01
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