bifidobacterium longum subsp infantis bi 26  (ATCC)

Journal: Gut Pathogens

Article Title: Strain-specific effects of probiotics on depression and anxiety: a meta-analysis

doi: 10.1186/s13099-024-00634-8

Figure Lengend Snippet: Studies characteristics

Article Snippet: Yamanbaeva, 2023 [ ] , RCT/4 weeks , 14/18 , Patients with depression, 37 , Probiotic capsule (1.0 × 10 9 CFU/g): Streptococcus thermophilus NCIMB 30,438 Bifidobacterium breve NCIMB 30,441 Bifidobacterium longum NCIMB 30,435 Bifidobacterium infantis NCIMB 30,436 Lactobacillus acidophilus NCIMB 30,442 Lactobacillus plantarum NCIMB 30,437 Lactobacillus paracasei NCIMB 30,439 Lactobacillus delbrueckii subsp Bulgaricus NCIMB 30,440 , Placebo with no probiotic bacteria , HAMD.

Techniques: Control, Bacteria, Maltodextrin, Capsules

Journal: Heliyon

Article Title: Ex-vivo investigation of probiotic bacterial adhesion to the intestinal mucus

doi: 10.1016/j.heliyon.2024.e36339

Figure Lengend Snippet: Morphological characteristics of the 5 bacterial strains: Lactiplantibacillus plantarum , Escherichia coli , Faecalibacterium duncaniae , Bifidobacterium infantis and Bifidobacterium longum . For SEM observation, fresh bacterial cultures collected at the end of the exponential phase of their growth were deposited and fixed on silicium wafer plates. The cell dimensions of bacteria were measured from SEM images. Each strain was measured randomly from 10 cells for their length and diameter. The min-max values were reported.

Article Snippet: Bifidobacterium longum Reuter BAA-999TM – Strain designation BB536 and Bifidobacterium longum subsp. infantis Reuter 15697TM – strain designation S12 were grown in modified reinforced clostridium broth medium (BK094HA – Biokar Diagnostics – Allonne, France) at 37 °C, inside an anaerobic chamber (Bactron I-2, Sheldon Manufacturing, USA) filled with 85 % N 2 , 5 % H 2 and 10 % CO 2 (v/v/v).

Techniques: Bacteria

Journal: Heliyon

Article Title: Ex-vivo investigation of probiotic bacterial adhesion to the intestinal mucus

doi: 10.1016/j.heliyon.2024.e36339

Figure Lengend Snippet: Scanning electron microscopic images of the 5 bacterial strains a. Escherichia. coli , b. Lactiplantibacillus plantarum , c. Faecalibacterium duncaniae , d. Bifidobacterium longum infantis , e. Bifidobacterium longum adhered to the pig intestinal surface. Pig intestine samples were previously treated to remove the gut microbiota and impurities in order to keep a layer of mucus on the intestinal surface before the deposition of the bacteria of interest. The bacteria were harvested at the end of the exponential phase of growth. 1 mL of bacterial culture (OD 600 = 1.0) was deposited on each piece of the intestine (1 × 1 cm 2 ) and let to adhere for 5 min. The samples were then washed twice with 1 mL of PBS for each washing step before fixation and critical point drying steps. The number 1, 2 and 3 refer to the images of bacteria on small intestinal surface, large intestinal surfaces, and their single cells, respectively.

Article Snippet: Bifidobacterium longum Reuter BAA-999TM – Strain designation BB536 and Bifidobacterium longum subsp. infantis Reuter 15697TM – strain designation S12 were grown in modified reinforced clostridium broth medium (BK094HA – Biokar Diagnostics – Allonne, France) at 37 °C, inside an anaerobic chamber (Bactron I-2, Sheldon Manufacturing, USA) filled with 85 % N 2 , 5 % H 2 and 10 % CO 2 (v/v/v).

Techniques: Bacteria

Journal: Heliyon

Article Title: Ex-vivo investigation of probiotic bacterial adhesion to the intestinal mucus

doi: 10.1016/j.heliyon.2024.e36339

Figure Lengend Snippet: Agglomeration of bacteria with intestinal mucus referred to as bacteria – mucus agglomeration index. For each bacterial strain placed in contact with fresh mucus, the experiments were performed on normalized concentration. The Latin letters a, b and c distinguish the difference between the 5 strains for their agglomeration ability with small intestinal mucus using one-way ANOVA with Tukey's test (p < 0.05). Similarly, the Latin letters a', b' and c' refer to their agglomeration ability with the large intestinal mucus. The difference between the agglomeration of each strain with SI and LI mucus was further analyzed by the student's t-test (p < 0.05). Corresponding results are indicated by the Greek letters α and β, followed by a number distinguishing each of the 5 strains.

Article Snippet: Bifidobacterium longum Reuter BAA-999TM – Strain designation BB536 and Bifidobacterium longum subsp. infantis Reuter 15697TM – strain designation S12 were grown in modified reinforced clostridium broth medium (BK094HA – Biokar Diagnostics – Allonne, France) at 37 °C, inside an anaerobic chamber (Bactron I-2, Sheldon Manufacturing, USA) filled with 85 % N 2 , 5 % H 2 and 10 % CO 2 (v/v/v).

Techniques: Bacteria, Concentration Assay

Journal: Heliyon

Article Title: Ex-vivo investigation of probiotic bacterial adhesion to the intestinal mucus

doi: 10.1016/j.heliyon.2024.e36339

Figure Lengend Snippet: Adhesion of viable bacteria to intestinal mucus layer coated on microtiter plate referred to as viable bacteria – mucus adhesion index. The Latin letters a, b and c distinguish the difference between the 5 strains for their adhesion to small intestinal mucus using one-way ANOVA with Tukey's test (p < 0.05). Similarly, the Latin letters a', b' and c' refer to their adhesion to the large intestinal mucus. The difference between the adhesion of each strain to SI and LI mucus was further analyzed by the student's t-test (p < 0.05). Corresponding results are indicated by the Greek letters α and β, followed by a number distinguishing each of the 5 strains.

Article Snippet: Bifidobacterium longum Reuter BAA-999TM – Strain designation BB536 and Bifidobacterium longum subsp. infantis Reuter 15697TM – strain designation S12 were grown in modified reinforced clostridium broth medium (BK094HA – Biokar Diagnostics – Allonne, France) at 37 °C, inside an anaerobic chamber (Bactron I-2, Sheldon Manufacturing, USA) filled with 85 % N 2 , 5 % H 2 and 10 % CO 2 (v/v/v).

Techniques: Bacteria