brain heart infusion bhi agar plate  (ATCC)

Journal: BMC Oral Health

Article Title: Evaluation methods of artificial demineralization protocols for coronal dentin: a systematic review of laboratory studies

doi: 10.1186/s12903-025-05885-8

Figure Lengend Snippet: Assessment methods for bacterial model in combined protocol, the aim of usage, description, and unit of measurement

Article Snippet: Steier et al . [ ] (2022) , • CFU • CLSM • FISH , • To measure the bacterial counts which used to examine the growth kinetics of biofilms • To analyze the invasion of bacterial biofilms into the dentinal tubules • For visualization of bacteria on enamel and dentin , • Biofilms were grown for 7 days on dentin discs. Dentin biofilms were collected in 1 mL of sterile BHI broth (pH 7.4) and cultured for 24 h at 37 ◦C. 100 L of broth were centrifuged five times in 100 L of PBS inside Eppendorf tubes. Each diluted specimen was plated on selective BHI agar plates in 5 µl and incubated for 24 h • Images were captured at various magnification and an electronic zoom of 6.3 times. Vertical sectioning at 0.97-m intervals through the biofilm produced a Z-series of optical sections - , • Number of microbial colony-forming units per milliliter (CFU/mL) - -.

Techniques: Confocal Laser Scanning Microscopy, Fluorescence In Situ Hybridization, Bacteria, Sterility, Cell Culture, Incubation, Produced