bglii  (TaKaRa)

 
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  • 99
    Name:
    Bgl II
    Description:
    A | GATC TT CTAG | A
    Catalog Number:
    1021b
    Price:
    None
    Size:
    10 000 Units
    Category:
    BglII Restriction enzymes Cloning
    Buy from Supplier


    Structured Review

    TaKaRa bglii
    Schematic diagram of vector construction. 1, PCR products of upstream homologous arm 2A with AscI and <t>BglII,</t> cloned into the <t>pMD18-T</t> vector and named pMD18-T-2A. pMD18-T-2A was digested with AscI and BglII endonucleases and inserted into the pGBHCupp
    A | GATC TT CTAG | A
    https://www.bioz.com/result/bglii/product/TaKaRa
    Average 99 stars, based on 249 article reviews
    Price from $9.99 to $1999.99
    bglii - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Chromosomal Insertions in the Lactobacillus caseiupp Gene That Are Useful for Vaccine Expression"

    Article Title: Chromosomal Insertions in the Lactobacillus caseiupp Gene That Are Useful for Vaccine Expression

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.00175-14

    Schematic diagram of vector construction. 1, PCR products of upstream homologous arm 2A with AscI and BglII, cloned into the pMD18-T vector and named pMD18-T-2A. pMD18-T-2A was digested with AscI and BglII endonucleases and inserted into the pGBHCupp
    Figure Legend Snippet: Schematic diagram of vector construction. 1, PCR products of upstream homologous arm 2A with AscI and BglII, cloned into the pMD18-T vector and named pMD18-T-2A. pMD18-T-2A was digested with AscI and BglII endonucleases and inserted into the pGBHCupp

    Techniques Used: Plasmid Preparation, Polymerase Chain Reaction, Clone Assay

    2) Product Images from "Lotus Leaf Aqueous Extract Reduces Visceral Fat Mass and Ameliorates Insulin Resistance in HFD-Induced Obese Rats by Regulating PPARγ2 Expression"

    Article Title: Lotus Leaf Aqueous Extract Reduces Visceral Fat Mass and Ameliorates Insulin Resistance in HFD-Induced Obese Rats by Regulating PPARγ2 Expression

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2017.00409

    Verification of pGL3-Enhancer-PPARγ2 (625 bp)-Luc plasmid by electrophoresis. Lane 1: DNA marker DL2000. Lane 2: Inserting fragment from pGL3-PPARγ2 (625 bp)-Luc digested by KpnI and BglII (625 bp). Lane 3: Vector fragment from pGL3-Enhancer-Luc digested by KpnI and BglII (5064 bp). Lane 4: The constructed pGL3-Enhancer-PPARγ2 (625 bp)-Luc plasmid (5689 bp). Lane 5: The constructed pGL3-Enhancer-PPARγ2 (625 bp)-Luc digested by KpnI and BglII (5064/625 bp). Lane 6: DNA marker III.
    Figure Legend Snippet: Verification of pGL3-Enhancer-PPARγ2 (625 bp)-Luc plasmid by electrophoresis. Lane 1: DNA marker DL2000. Lane 2: Inserting fragment from pGL3-PPARγ2 (625 bp)-Luc digested by KpnI and BglII (625 bp). Lane 3: Vector fragment from pGL3-Enhancer-Luc digested by KpnI and BglII (5064 bp). Lane 4: The constructed pGL3-Enhancer-PPARγ2 (625 bp)-Luc plasmid (5689 bp). Lane 5: The constructed pGL3-Enhancer-PPARγ2 (625 bp)-Luc digested by KpnI and BglII (5064/625 bp). Lane 6: DNA marker III.

    Techniques Used: Plasmid Preparation, Electrophoresis, Marker, Construct

    3) Product Images from "Construction of the POT1 promoter report gene vector, and the effect and underlying mechanism of the POT1 promoter in regulating telomerase and telomere length"

    Article Title: Construction of the POT1 promoter report gene vector, and the effect and underlying mechanism of the POT1 promoter in regulating telomerase and telomere length

    Journal: Oncology Letters

    doi: 10.3892/ol.2017.7127

    Identification of the recombinant plasmid POT1-promoter-2. (A) Electrophoresis of BglII and HindIII digested products. M, molecular weight marker (200–5,000 bp); lane 1 and 2, enzyme digestion products (5,057 and 461 bp). (B) Electrophoresis of the PCR products. M, molecular weight marker (100–600 bp); lane 1–4, the PCR products (461 bp) of the POT1-promoter-2 plasmid using primers for POT1-promoter-2. (C) Sequencing results of POT1-promoter-2. POT1, protection of telomere 1; PCR, polymerase chain reaction; bp, base pairs.
    Figure Legend Snippet: Identification of the recombinant plasmid POT1-promoter-2. (A) Electrophoresis of BglII and HindIII digested products. M, molecular weight marker (200–5,000 bp); lane 1 and 2, enzyme digestion products (5,057 and 461 bp). (B) Electrophoresis of the PCR products. M, molecular weight marker (100–600 bp); lane 1–4, the PCR products (461 bp) of the POT1-promoter-2 plasmid using primers for POT1-promoter-2. (C) Sequencing results of POT1-promoter-2. POT1, protection of telomere 1; PCR, polymerase chain reaction; bp, base pairs.

    Techniques Used: Recombinant, Plasmid Preparation, Electrophoresis, Molecular Weight, Marker, Polymerase Chain Reaction, Sequencing

    Identification of the recombinant plasmid POT1-promoter-1. (A) Electrophoresis of BglII and HindIII digested products. M, molecular weight marker (200–5,000 bp); lane 1–3, enzyme digestion products (5,057 and 207 bp). (B) Electrophoresis of the PCR products. M, molecular weight marker (100–600 bp); lane 1–4, the PCR products (207 bp) of the POT1-promoter-1 plasmid using primers for POT1-promoter-1. (C) Sequencing results of POT1-promoter-1. POT1, protection of telomere 1; PCR, polymerase chain reaction; bp, base pairs.
    Figure Legend Snippet: Identification of the recombinant plasmid POT1-promoter-1. (A) Electrophoresis of BglII and HindIII digested products. M, molecular weight marker (200–5,000 bp); lane 1–3, enzyme digestion products (5,057 and 207 bp). (B) Electrophoresis of the PCR products. M, molecular weight marker (100–600 bp); lane 1–4, the PCR products (207 bp) of the POT1-promoter-1 plasmid using primers for POT1-promoter-1. (C) Sequencing results of POT1-promoter-1. POT1, protection of telomere 1; PCR, polymerase chain reaction; bp, base pairs.

    Techniques Used: Recombinant, Plasmid Preparation, Electrophoresis, Molecular Weight, Marker, Polymerase Chain Reaction, Sequencing

    4) Product Images from "Establishment of DNA-DNA Interactions by the Cohesin Ring"

    Article Title: Establishment of DNA-DNA Interactions by the Cohesin Ring

    Journal: Cell

    doi: 10.1016/j.cell.2017.12.021

    Second-DNA Capture is Topological in Nature, Related to Figure 2 (A) Cohesin must topologically embrace dsDNA to mediate second-DNA capture. Protocol 1 reactions were carried out with ‘closed’ (C) or ‘linear’ (L) topology dsDNA beads. The gel image and graph show recovery of free ssDNA. The graph shows means and standard deviation from three independent experiments (WT cohesin) or the range of recovered ssDNA from two independent experiments (1B3B cohesin). (B) A DNA release experiment as shown in Figure 2 A was carried out using 1B3B cohesin. (C) Schematic of a DNA release experiment following protocol 2 s DNA capture. The ssDNA substrate was converted to dsDNA by DNA synthesis following capture. Then either of the two circular DNAs was digested with unique restriction enzymes, PstI (DNA beads) or BglII (free dsDNA). Recovered DNAs at the indicated stages of the experiment were analyzed by agarose gel electrophoresis. Input, bead bound (B) and supernatant (S) fractions are shown. (D) TEV cleavage of cohesin following second-DNA capture using protocol 2. The gel shows a representative image of input and recovered DNA, using WT and TEV cleavable (21TEV) cohesin, without or with TEV protease (TEV) treatment. After TEV protease treatment, the beads were washed with high salt buffer and recovered DNA was analyzed. The graph depicts the means and standard deviations from three independent experiments.
    Figure Legend Snippet: Second-DNA Capture is Topological in Nature, Related to Figure 2 (A) Cohesin must topologically embrace dsDNA to mediate second-DNA capture. Protocol 1 reactions were carried out with ‘closed’ (C) or ‘linear’ (L) topology dsDNA beads. The gel image and graph show recovery of free ssDNA. The graph shows means and standard deviation from three independent experiments (WT cohesin) or the range of recovered ssDNA from two independent experiments (1B3B cohesin). (B) A DNA release experiment as shown in Figure 2 A was carried out using 1B3B cohesin. (C) Schematic of a DNA release experiment following protocol 2 s DNA capture. The ssDNA substrate was converted to dsDNA by DNA synthesis following capture. Then either of the two circular DNAs was digested with unique restriction enzymes, PstI (DNA beads) or BglII (free dsDNA). Recovered DNAs at the indicated stages of the experiment were analyzed by agarose gel electrophoresis. Input, bead bound (B) and supernatant (S) fractions are shown. (D) TEV cleavage of cohesin following second-DNA capture using protocol 2. The gel shows a representative image of input and recovered DNA, using WT and TEV cleavable (21TEV) cohesin, without or with TEV protease (TEV) treatment. After TEV protease treatment, the beads were washed with high salt buffer and recovered DNA was analyzed. The graph depicts the means and standard deviations from three independent experiments.

    Techniques Used: Standard Deviation, DNA Synthesis, Agarose Gel Electrophoresis

    5) Product Images from "Lacanobia oleracea nucleopolyhedrovirus (LaolNPV): A new European species of alphabaculovirus with a narrow host range"

    Article Title: Lacanobia oleracea nucleopolyhedrovirus (LaolNPV): A new European species of alphabaculovirus with a narrow host range

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0176171

    Restriction endonuclease analysis of LaolNPV DNA. Restriction endonuclease profiles following digestion of A) genomic DNAs of the seven isolates of LaolNPV collected in alfalfa crops nearby Montpellier with BglII, EcoRI and PstI enzymes, B) genomic DNAs of Chrysodeixis chalcites NPV (Chch), Helicoverpa armigera SNPV (Hear), Spodoptera littoralis (Spli), Spodopera exigua MNPV (Se) and the virus under study (LaolNPV) with BamHI, BglII, EcoRI and PstI, and C) genomic DNAs of Mamestra brassicae MNPV (Mb), Mamestra configurata NPV-A (MacoA), Mamestra configurata NPV-B (MacoB) and LaolNPV with BglII, EcoRI and PstI.
    Figure Legend Snippet: Restriction endonuclease analysis of LaolNPV DNA. Restriction endonuclease profiles following digestion of A) genomic DNAs of the seven isolates of LaolNPV collected in alfalfa crops nearby Montpellier with BglII, EcoRI and PstI enzymes, B) genomic DNAs of Chrysodeixis chalcites NPV (Chch), Helicoverpa armigera SNPV (Hear), Spodoptera littoralis (Spli), Spodopera exigua MNPV (Se) and the virus under study (LaolNPV) with BamHI, BglII, EcoRI and PstI, and C) genomic DNAs of Mamestra brassicae MNPV (Mb), Mamestra configurata NPV-A (MacoA), Mamestra configurata NPV-B (MacoB) and LaolNPV with BglII, EcoRI and PstI.

    Techniques Used:

    Related Articles

    Clone Assay:

    Article Title: In Vitro Evaluation of Chitosan-DNA Plasmid Complex Encoding Jembrana Disease Virus Env-TM Protein as a Vaccine Candidate
    Article Snippet: .. Then, the transgene was chemically synthesised with restriction sites of Bgl II and EcoR I and cloned into the pEGFP-C1 vector (Clontech) by Gene Universal Inc., resulting in pEGFP-env-tm JDV ( ). ..

    Article Title: Expression of a biotin acceptor peptide-containing protein with potential incorporation on the lentiviral envelope as a viral surface engineering platform
    Article Snippet: .. For cloning of the amplicon, both pDisplay and GS-BAP DNA fragments were digested by Bgl II and Sal I (Takara, Japan) and the corresponding bands were gel purified by GeneJET™ Gel Extraction Kit (Fermentas, Lithuania). .. Subsequently, the purified insert fragment (GS-BAP) was cloned into the Bgl II-Sal I restriction sites of pDisplay, downstream of HA-tag and in frame with myc epitope by T4 DNA ligase to make the final pDis-GS-BAP plasmid ( ).

    Amplification:

    Article Title: Expression of a biotin acceptor peptide-containing protein with potential incorporation on the lentiviral envelope as a viral surface engineering platform
    Article Snippet: .. For cloning of the amplicon, both pDisplay and GS-BAP DNA fragments were digested by Bgl II and Sal I (Takara, Japan) and the corresponding bands were gel purified by GeneJET™ Gel Extraction Kit (Fermentas, Lithuania). .. Subsequently, the purified insert fragment (GS-BAP) was cloned into the Bgl II-Sal I restriction sites of pDisplay, downstream of HA-tag and in frame with myc epitope by T4 DNA ligase to make the final pDis-GS-BAP plasmid ( ).

    Agarose Gel Electrophoresis:

    Article Title: Construction of eukaryotic expression vector of TSLC1 gene
    Article Snippet: .. Extraction and identification of pMD19-T simple-TSLC1 recombinant plasmid pMD19-T Simple-TSLC1 recombinant plasmid was extracted from E. coli JM109 with Mini Plasmid Purification Kit according to the instructions; 1 µg of recombinant plasmid was digested by restriction enzyme Bgl II and EcoR I for 37°C, 8 h; 5 µl of digestion product was analysed with agarose gel electrophoresis; the positive plasmid was sequenced by TaKaRa Biotechnology Co, Ltd (Dalian, China). .. Subclone of pIRES2-EGFP-TSLC1 recombinant plasmid

    Article Title: Construction of eukaryotic expression vector of TSLC1 gene
    Article Snippet: .. Material and methods Normal foreskin acrobystia was obtained from patients admitted to Guangdong Medical College Hospital emergency operating room with written consent; TRIzol was purchased from Invitrogen (Carlsbad, CA, USA); Escherichia coli JM109 and plasmid pIRES2-EGFP were kind gifts from the biochemistry laboratory of Guangdong Medical College; High Fidelity PrimeScript™ RT-PCR Kit, DL2,000 DNA Marker, λ-Hind III DNA Marker, pMD19-T Simple vector, DNA A-Tailing Kit, DNA Ligation Kit, Agarose Gel DNA Purification Kit, restriction endonucleases EcoR I and Bgl II were purchased from TaKaRa Biotechnology Co, Ltd (Dalian, China). .. Mini Plasmid Purification Kit was obtained from Beyotime Institute of Biotechnology (Haimen, China).

    DNA Ligation:

    Article Title: Construction of eukaryotic expression vector of TSLC1 gene
    Article Snippet: .. Material and methods Normal foreskin acrobystia was obtained from patients admitted to Guangdong Medical College Hospital emergency operating room with written consent; TRIzol was purchased from Invitrogen (Carlsbad, CA, USA); Escherichia coli JM109 and plasmid pIRES2-EGFP were kind gifts from the biochemistry laboratory of Guangdong Medical College; High Fidelity PrimeScript™ RT-PCR Kit, DL2,000 DNA Marker, λ-Hind III DNA Marker, pMD19-T Simple vector, DNA A-Tailing Kit, DNA Ligation Kit, Agarose Gel DNA Purification Kit, restriction endonucleases EcoR I and Bgl II were purchased from TaKaRa Biotechnology Co, Ltd (Dalian, China). .. Mini Plasmid Purification Kit was obtained from Beyotime Institute of Biotechnology (Haimen, China).

    Marker:

    Article Title: Construction of eukaryotic expression vector of TSLC1 gene
    Article Snippet: .. Material and methods Normal foreskin acrobystia was obtained from patients admitted to Guangdong Medical College Hospital emergency operating room with written consent; TRIzol was purchased from Invitrogen (Carlsbad, CA, USA); Escherichia coli JM109 and plasmid pIRES2-EGFP were kind gifts from the biochemistry laboratory of Guangdong Medical College; High Fidelity PrimeScript™ RT-PCR Kit, DL2,000 DNA Marker, λ-Hind III DNA Marker, pMD19-T Simple vector, DNA A-Tailing Kit, DNA Ligation Kit, Agarose Gel DNA Purification Kit, restriction endonucleases EcoR I and Bgl II were purchased from TaKaRa Biotechnology Co, Ltd (Dalian, China). .. Mini Plasmid Purification Kit was obtained from Beyotime Institute of Biotechnology (Haimen, China).

    Construct:

    Article Title: Lotus Leaf Aqueous Extract Reduces Visceral Fat Mass and Ameliorates Insulin Resistance in HFD-Induced Obese Rats by Regulating PPARγ2 Expression
    Article Snippet: .. In brief, pGL3-PPARγ2 (625 bp)-Luc, which contained the human PPARγ2 gene 5′-promoter fragment spanning -615 to +10 bp (+1 indicates the transcription start site) and was constructed previously in our laboratory, was digested by the restriction enzymes KpnI and BglII (Takara, Japan) to get the 625 bp PPARγ2 gene promoter inserting fragment. .. The fragment was then inserted into pGL3-Enhancer-Luc vector to yield pGL3-Enhancer-PPARγ2 (625 bp)-Luc plasmid.

    Purification:

    Article Title: Construction of eukaryotic expression vector of TSLC1 gene
    Article Snippet: .. Extraction and identification of pMD19-T simple-TSLC1 recombinant plasmid pMD19-T Simple-TSLC1 recombinant plasmid was extracted from E. coli JM109 with Mini Plasmid Purification Kit according to the instructions; 1 µg of recombinant plasmid was digested by restriction enzyme Bgl II and EcoR I for 37°C, 8 h; 5 µl of digestion product was analysed with agarose gel electrophoresis; the positive plasmid was sequenced by TaKaRa Biotechnology Co, Ltd (Dalian, China). .. Subclone of pIRES2-EGFP-TSLC1 recombinant plasmid

    Article Title: Expression of a biotin acceptor peptide-containing protein with potential incorporation on the lentiviral envelope as a viral surface engineering platform
    Article Snippet: .. For cloning of the amplicon, both pDisplay and GS-BAP DNA fragments were digested by Bgl II and Sal I (Takara, Japan) and the corresponding bands were gel purified by GeneJET™ Gel Extraction Kit (Fermentas, Lithuania). .. Subsequently, the purified insert fragment (GS-BAP) was cloned into the Bgl II-Sal I restriction sites of pDisplay, downstream of HA-tag and in frame with myc epitope by T4 DNA ligase to make the final pDis-GS-BAP plasmid ( ).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Construction of eukaryotic expression vector of TSLC1 gene
    Article Snippet: .. Material and methods Normal foreskin acrobystia was obtained from patients admitted to Guangdong Medical College Hospital emergency operating room with written consent; TRIzol was purchased from Invitrogen (Carlsbad, CA, USA); Escherichia coli JM109 and plasmid pIRES2-EGFP were kind gifts from the biochemistry laboratory of Guangdong Medical College; High Fidelity PrimeScript™ RT-PCR Kit, DL2,000 DNA Marker, λ-Hind III DNA Marker, pMD19-T Simple vector, DNA A-Tailing Kit, DNA Ligation Kit, Agarose Gel DNA Purification Kit, restriction endonucleases EcoR I and Bgl II were purchased from TaKaRa Biotechnology Co, Ltd (Dalian, China). .. Mini Plasmid Purification Kit was obtained from Beyotime Institute of Biotechnology (Haimen, China).

    Sequencing:

    Article Title: Construction of the POT1 promoter report gene vector, and the effect and underlying mechanism of the POT1 promoter in regulating telomerase and telomere length
    Article Snippet: .. Subsequently, the pGL3-control plasmid (Promega Corporation, Madison, WI, USA) and PCR products containing the desired sequence were double-digested with BglII and HindIII , and annealed together using T4 DNA ligase (Takara Biotechnology Co., Ltd.) at 16°C overnight. .. The ligation products were transformed into chemocompetent Escherichia coli DH5α cells (Tiangen Biotech Co., Ltd., Beijing, China) maintained in LB medium (Shanghai Kehua Bio-Engineering Co., Ltd., Shanghai, China) and bacterial culture medium containing ampicillin (Tiangen Biotech Co., Ltd., Beijing, China) to select for the recombinant plasmid-positive colonies.

    Generated:

    Article Title: Comparative genomic analysis of two emergent human adenovirus type 14 respiratory pathogen isolates in China reveals similar yet divergent genomes
    Article Snippet: .. Genome type identification In silico restriction maps of available genome sequences from all HAdV-14 strains CHN/GZ01/2010, 303600, CHN/BJ430, CHN2012, and prototype de Wit were generated using the Vector NTI 10.3.0 software (Invitrogen Corp.; San Diego, CA, USA) as described in earlier studies., , These included profiles from restriction enzymes Bam HI, Bcl I, Bgl l, Bgl II, Bst EII, Eco RI, Hin dIII, Hpa I, Sal l, Sma I, Xba I, and Xho I (TaKaRa Corp.; China), all of which were chosen in order to be consistent with the original nomenclature system devised by Li and colleagues. .. Genome typing of these strains was determined by comparing its in silico RE profiles with other HAdV-14 genome types.,

    In Silico:

    Article Title: Comparative genomic analysis of two emergent human adenovirus type 14 respiratory pathogen isolates in China reveals similar yet divergent genomes
    Article Snippet: .. Genome type identification In silico restriction maps of available genome sequences from all HAdV-14 strains CHN/GZ01/2010, 303600, CHN/BJ430, CHN2012, and prototype de Wit were generated using the Vector NTI 10.3.0 software (Invitrogen Corp.; San Diego, CA, USA) as described in earlier studies., , These included profiles from restriction enzymes Bam HI, Bcl I, Bgl l, Bgl II, Bst EII, Eco RI, Hin dIII, Hpa I, Sal l, Sma I, Xba I, and Xho I (TaKaRa Corp.; China), all of which were chosen in order to be consistent with the original nomenclature system devised by Li and colleagues. .. Genome typing of these strains was determined by comparing its in silico RE profiles with other HAdV-14 genome types.,

    DNA Purification:

    Article Title: Construction of eukaryotic expression vector of TSLC1 gene
    Article Snippet: .. Material and methods Normal foreskin acrobystia was obtained from patients admitted to Guangdong Medical College Hospital emergency operating room with written consent; TRIzol was purchased from Invitrogen (Carlsbad, CA, USA); Escherichia coli JM109 and plasmid pIRES2-EGFP were kind gifts from the biochemistry laboratory of Guangdong Medical College; High Fidelity PrimeScript™ RT-PCR Kit, DL2,000 DNA Marker, λ-Hind III DNA Marker, pMD19-T Simple vector, DNA A-Tailing Kit, DNA Ligation Kit, Agarose Gel DNA Purification Kit, restriction endonucleases EcoR I and Bgl II were purchased from TaKaRa Biotechnology Co, Ltd (Dalian, China). .. Mini Plasmid Purification Kit was obtained from Beyotime Institute of Biotechnology (Haimen, China).

    Polymerase Chain Reaction:

    Article Title: Construction of the POT1 promoter report gene vector, and the effect and underlying mechanism of the POT1 promoter in regulating telomerase and telomere length
    Article Snippet: .. Subsequently, the pGL3-control plasmid (Promega Corporation, Madison, WI, USA) and PCR products containing the desired sequence were double-digested with BglII and HindIII , and annealed together using T4 DNA ligase (Takara Biotechnology Co., Ltd.) at 16°C overnight. .. The ligation products were transformed into chemocompetent Escherichia coli DH5α cells (Tiangen Biotech Co., Ltd., Beijing, China) maintained in LB medium (Shanghai Kehua Bio-Engineering Co., Ltd., Shanghai, China) and bacterial culture medium containing ampicillin (Tiangen Biotech Co., Ltd., Beijing, China) to select for the recombinant plasmid-positive colonies.

    Gel Extraction:

    Article Title: Expression of a biotin acceptor peptide-containing protein with potential incorporation on the lentiviral envelope as a viral surface engineering platform
    Article Snippet: .. For cloning of the amplicon, both pDisplay and GS-BAP DNA fragments were digested by Bgl II and Sal I (Takara, Japan) and the corresponding bands were gel purified by GeneJET™ Gel Extraction Kit (Fermentas, Lithuania). .. Subsequently, the purified insert fragment (GS-BAP) was cloned into the Bgl II-Sal I restriction sites of pDisplay, downstream of HA-tag and in frame with myc epitope by T4 DNA ligase to make the final pDis-GS-BAP plasmid ( ).

    Recombinant:

    Article Title: Construction of eukaryotic expression vector of TSLC1 gene
    Article Snippet: .. Extraction and identification of pMD19-T simple-TSLC1 recombinant plasmid pMD19-T Simple-TSLC1 recombinant plasmid was extracted from E. coli JM109 with Mini Plasmid Purification Kit according to the instructions; 1 µg of recombinant plasmid was digested by restriction enzyme Bgl II and EcoR I for 37°C, 8 h; 5 µl of digestion product was analysed with agarose gel electrophoresis; the positive plasmid was sequenced by TaKaRa Biotechnology Co, Ltd (Dalian, China). .. Subclone of pIRES2-EGFP-TSLC1 recombinant plasmid

    Plasmid Preparation:

    Article Title: Construction of eukaryotic expression vector of TSLC1 gene
    Article Snippet: .. Extraction and identification of pMD19-T simple-TSLC1 recombinant plasmid pMD19-T Simple-TSLC1 recombinant plasmid was extracted from E. coli JM109 with Mini Plasmid Purification Kit according to the instructions; 1 µg of recombinant plasmid was digested by restriction enzyme Bgl II and EcoR I for 37°C, 8 h; 5 µl of digestion product was analysed with agarose gel electrophoresis; the positive plasmid was sequenced by TaKaRa Biotechnology Co, Ltd (Dalian, China). .. Subclone of pIRES2-EGFP-TSLC1 recombinant plasmid

    Article Title: Comparative genomic analysis of two emergent human adenovirus type 14 respiratory pathogen isolates in China reveals similar yet divergent genomes
    Article Snippet: .. Genome type identification In silico restriction maps of available genome sequences from all HAdV-14 strains CHN/GZ01/2010, 303600, CHN/BJ430, CHN2012, and prototype de Wit were generated using the Vector NTI 10.3.0 software (Invitrogen Corp.; San Diego, CA, USA) as described in earlier studies., , These included profiles from restriction enzymes Bam HI, Bcl I, Bgl l, Bgl II, Bst EII, Eco RI, Hin dIII, Hpa I, Sal l, Sma I, Xba I, and Xho I (TaKaRa Corp.; China), all of which were chosen in order to be consistent with the original nomenclature system devised by Li and colleagues. .. Genome typing of these strains was determined by comparing its in silico RE profiles with other HAdV-14 genome types.,

    Article Title: In Vitro Evaluation of Chitosan-DNA Plasmid Complex Encoding Jembrana Disease Virus Env-TM Protein as a Vaccine Candidate
    Article Snippet: .. Then, the transgene was chemically synthesised with restriction sites of Bgl II and EcoR I and cloned into the pEGFP-C1 vector (Clontech) by Gene Universal Inc., resulting in pEGFP-env-tm JDV ( ). ..

    Article Title: Construction of eukaryotic expression vector of TSLC1 gene
    Article Snippet: .. Material and methods Normal foreskin acrobystia was obtained from patients admitted to Guangdong Medical College Hospital emergency operating room with written consent; TRIzol was purchased from Invitrogen (Carlsbad, CA, USA); Escherichia coli JM109 and plasmid pIRES2-EGFP were kind gifts from the biochemistry laboratory of Guangdong Medical College; High Fidelity PrimeScript™ RT-PCR Kit, DL2,000 DNA Marker, λ-Hind III DNA Marker, pMD19-T Simple vector, DNA A-Tailing Kit, DNA Ligation Kit, Agarose Gel DNA Purification Kit, restriction endonucleases EcoR I and Bgl II were purchased from TaKaRa Biotechnology Co, Ltd (Dalian, China). .. Mini Plasmid Purification Kit was obtained from Beyotime Institute of Biotechnology (Haimen, China).

    Article Title: Construction of the POT1 promoter report gene vector, and the effect and underlying mechanism of the POT1 promoter in regulating telomerase and telomere length
    Article Snippet: .. Subsequently, the pGL3-control plasmid (Promega Corporation, Madison, WI, USA) and PCR products containing the desired sequence were double-digested with BglII and HindIII , and annealed together using T4 DNA ligase (Takara Biotechnology Co., Ltd.) at 16°C overnight. .. The ligation products were transformed into chemocompetent Escherichia coli DH5α cells (Tiangen Biotech Co., Ltd., Beijing, China) maintained in LB medium (Shanghai Kehua Bio-Engineering Co., Ltd., Shanghai, China) and bacterial culture medium containing ampicillin (Tiangen Biotech Co., Ltd., Beijing, China) to select for the recombinant plasmid-positive colonies.

    Article Title: Chromosomal Insertions in the Lactobacillus caseiupp Gene That Are Useful for Vaccine Expression
    Article Snippet: .. The pMD18-T-2A plasmid was digested with BglII (TaKaRa) and AscI (NEB). .. 2A was inserted into the pGBHCupp vector MCS site using the same restriction enzyme digestion, and this construct was named pGBHCupp-2A.

    Software:

    Article Title: Comparative genomic analysis of two emergent human adenovirus type 14 respiratory pathogen isolates in China reveals similar yet divergent genomes
    Article Snippet: .. Genome type identification In silico restriction maps of available genome sequences from all HAdV-14 strains CHN/GZ01/2010, 303600, CHN/BJ430, CHN2012, and prototype de Wit were generated using the Vector NTI 10.3.0 software (Invitrogen Corp.; San Diego, CA, USA) as described in earlier studies., , These included profiles from restriction enzymes Bam HI, Bcl I, Bgl l, Bgl II, Bst EII, Eco RI, Hin dIII, Hpa I, Sal l, Sma I, Xba I, and Xho I (TaKaRa Corp.; China), all of which were chosen in order to be consistent with the original nomenclature system devised by Li and colleagues. .. Genome typing of these strains was determined by comparing its in silico RE profiles with other HAdV-14 genome types.,

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    TaKaRa recombinant wild type bglii rbgliiwt
    Chromatogram of transglycosylation products by <t>rBGLIIwt.</t> HPLC analysis of transglycosylation products using purified recombinant wild-type <t>BGLII</t> in E. coli . a The chromatogram generated by the Prominence HPLC system. In the standard chromatogram, G4, G3, G2, and G1 represent cellotetraose, cellotriose, cellobiose, and glucose peaks, respectively. Other β-disaccharides, α-sophorose, laminaribiose, and gentiobiose are represented by Sp, Lm, and Ge, respectively. b The chromatogram of size exclusion chromatography. Cello-oligosaccharides were used as the standard substance. Putative transglycosylation products are indicated by arrows . Asterisk refers to the peak of a buffer component in the reactant
    Recombinant Wild Type Bglii Rbgliiwt, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant wild type bglii rbgliiwt/product/TaKaRa
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant wild type bglii rbgliiwt - by Bioz Stars, 2020-09
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    88
    TaKaRa bglii sali site
    Splicing regulation of MBNL and CELF proteins. ( A ) Structure of chloride channel minigenes. Both human CLCN1/ClC-1 and mouse Clcn1 minigenes were subcloned between the <t>BglII</t> and <t>SalI</t> sites of pEGFP-C1. Black boxes represent exons of the minigenes. Arrows indicate the position of primers used in the splicing assays. Exon 6B is a human-specific exon and is absent in Clcn1 . ( B ) Splicing regulation of Clcn1 by MBNL and CELF proteins. Representative results of cellular splicing assays using the Clcn1 minigene in COS-7 cells. The upper bands correspond to a splice product containing exon 7A, whereas lower bands correspond to a splice product lacking exon 7A. Bar chart shows quantified results of exon 7A inclusion (mean ± SD, n = 3). Statistical significance was analyzed by analysis of variance (ANOVA) and Dunnett's multiple comparisons. All MBNL proteins and CELF proteins except for CUG-BP and ETR-3 showed significant differences (* P
    Bglii Sali Site, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Schematic diagram of vector construction. 1, PCR products of upstream homologous arm 2A with AscI and <t>BglII,</t> cloned into the <t>pMD18-T</t> vector and named pMD18-T-2A. pMD18-T-2A was digested with AscI and BglII endonucleases and inserted into the pGBHCupp
    Bglii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 249 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bglii/product/TaKaRa
    Average 99 stars, based on 249 article reviews
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    bglii - by Bioz Stars, 2020-09
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    Image Search Results


    Chromatogram of transglycosylation products by rBGLIIwt. HPLC analysis of transglycosylation products using purified recombinant wild-type BGLII in E. coli . a The chromatogram generated by the Prominence HPLC system. In the standard chromatogram, G4, G3, G2, and G1 represent cellotetraose, cellotriose, cellobiose, and glucose peaks, respectively. Other β-disaccharides, α-sophorose, laminaribiose, and gentiobiose are represented by Sp, Lm, and Ge, respectively. b The chromatogram of size exclusion chromatography. Cello-oligosaccharides were used as the standard substance. Putative transglycosylation products are indicated by arrows . Asterisk refers to the peak of a buffer component in the reactant

    Journal: Biotechnology for Biofuels

    Article Title: The impact of a single-nucleotide mutation of bgl2 on cellulase induction in a Trichoderma reesei mutant

    doi: 10.1186/s13068-015-0420-y

    Figure Lengend Snippet: Chromatogram of transglycosylation products by rBGLIIwt. HPLC analysis of transglycosylation products using purified recombinant wild-type BGLII in E. coli . a The chromatogram generated by the Prominence HPLC system. In the standard chromatogram, G4, G3, G2, and G1 represent cellotetraose, cellotriose, cellobiose, and glucose peaks, respectively. Other β-disaccharides, α-sophorose, laminaribiose, and gentiobiose are represented by Sp, Lm, and Ge, respectively. b The chromatogram of size exclusion chromatography. Cello-oligosaccharides were used as the standard substance. Putative transglycosylation products are indicated by arrows . Asterisk refers to the peak of a buffer component in the reactant

    Article Snippet: After cultivation, cell lysate of harvested E. coli cells was prepared using xTracter Buffer (Takara-Bio, Shiga, Japan) and recombinant wild-type BGLII (rBGLIIwt) was purified by TALON® Metal Affinity Resins (Takara-Bio, Shiga, Japan) as described in the instructions.

    Techniques: High Performance Liquid Chromatography, Purification, Recombinant, Generated, Size-exclusion Chromatography

    Splicing regulation of MBNL and CELF proteins. ( A ) Structure of chloride channel minigenes. Both human CLCN1/ClC-1 and mouse Clcn1 minigenes were subcloned between the BglII and SalI sites of pEGFP-C1. Black boxes represent exons of the minigenes. Arrows indicate the position of primers used in the splicing assays. Exon 6B is a human-specific exon and is absent in Clcn1 . ( B ) Splicing regulation of Clcn1 by MBNL and CELF proteins. Representative results of cellular splicing assays using the Clcn1 minigene in COS-7 cells. The upper bands correspond to a splice product containing exon 7A, whereas lower bands correspond to a splice product lacking exon 7A. Bar chart shows quantified results of exon 7A inclusion (mean ± SD, n = 3). Statistical significance was analyzed by analysis of variance (ANOVA) and Dunnett's multiple comparisons. All MBNL proteins and CELF proteins except for CUG-BP and ETR-3 showed significant differences (* P

    Journal: Nucleic Acids Research

    Article Title: MBNL and CELF proteins regulate alternative splicing of the skeletal muscle chloride channel CLCN1

    doi: 10.1093/nar/gkp681

    Figure Lengend Snippet: Splicing regulation of MBNL and CELF proteins. ( A ) Structure of chloride channel minigenes. Both human CLCN1/ClC-1 and mouse Clcn1 minigenes were subcloned between the BglII and SalI sites of pEGFP-C1. Black boxes represent exons of the minigenes. Arrows indicate the position of primers used in the splicing assays. Exon 6B is a human-specific exon and is absent in Clcn1 . ( B ) Splicing regulation of Clcn1 by MBNL and CELF proteins. Representative results of cellular splicing assays using the Clcn1 minigene in COS-7 cells. The upper bands correspond to a splice product containing exon 7A, whereas lower bands correspond to a splice product lacking exon 7A. Bar chart shows quantified results of exon 7A inclusion (mean ± SD, n = 3). Statistical significance was analyzed by analysis of variance (ANOVA) and Dunnett's multiple comparisons. All MBNL proteins and CELF proteins except for CUG-BP and ETR-3 showed significant differences (* P

    Article Snippet: The minigene fragments in pGEM-T Easy were cleaved by BamHI and SalI and then subcloned into the BglII-SalI site of pEGFP-C1 (Clontech).

    Techniques:

    Schematic diagram of vector construction. 1, PCR products of upstream homologous arm 2A with AscI and BglII, cloned into the pMD18-T vector and named pMD18-T-2A. pMD18-T-2A was digested with AscI and BglII endonucleases and inserted into the pGBHCupp

    Journal: Applied and Environmental Microbiology

    Article Title: Chromosomal Insertions in the Lactobacillus caseiupp Gene That Are Useful for Vaccine Expression

    doi: 10.1128/AEM.00175-14

    Figure Lengend Snippet: Schematic diagram of vector construction. 1, PCR products of upstream homologous arm 2A with AscI and BglII, cloned into the pMD18-T vector and named pMD18-T-2A. pMD18-T-2A was digested with AscI and BglII endonucleases and inserted into the pGBHCupp

    Article Snippet: The pMD18-T-2A plasmid was digested with BglII (TaKaRa) and AscI (NEB).

    Techniques: Plasmid Preparation, Polymerase Chain Reaction, Clone Assay

    Verification of pGL3-Enhancer-PPARγ2 (625 bp)-Luc plasmid by electrophoresis. Lane 1: DNA marker DL2000. Lane 2: Inserting fragment from pGL3-PPARγ2 (625 bp)-Luc digested by KpnI and BglII (625 bp). Lane 3: Vector fragment from pGL3-Enhancer-Luc digested by KpnI and BglII (5064 bp). Lane 4: The constructed pGL3-Enhancer-PPARγ2 (625 bp)-Luc plasmid (5689 bp). Lane 5: The constructed pGL3-Enhancer-PPARγ2 (625 bp)-Luc digested by KpnI and BglII (5064/625 bp). Lane 6: DNA marker III.

    Journal: Frontiers in Pharmacology

    Article Title: Lotus Leaf Aqueous Extract Reduces Visceral Fat Mass and Ameliorates Insulin Resistance in HFD-Induced Obese Rats by Regulating PPARγ2 Expression

    doi: 10.3389/fphar.2017.00409

    Figure Lengend Snippet: Verification of pGL3-Enhancer-PPARγ2 (625 bp)-Luc plasmid by electrophoresis. Lane 1: DNA marker DL2000. Lane 2: Inserting fragment from pGL3-PPARγ2 (625 bp)-Luc digested by KpnI and BglII (625 bp). Lane 3: Vector fragment from pGL3-Enhancer-Luc digested by KpnI and BglII (5064 bp). Lane 4: The constructed pGL3-Enhancer-PPARγ2 (625 bp)-Luc plasmid (5689 bp). Lane 5: The constructed pGL3-Enhancer-PPARγ2 (625 bp)-Luc digested by KpnI and BglII (5064/625 bp). Lane 6: DNA marker III.

    Article Snippet: In brief, pGL3-PPARγ2 (625 bp)-Luc, which contained the human PPARγ2 gene 5′-promoter fragment spanning -615 to +10 bp (+1 indicates the transcription start site) and was constructed previously in our laboratory, was digested by the restriction enzymes KpnI and BglII (Takara, Japan) to get the 625 bp PPARγ2 gene promoter inserting fragment.

    Techniques: Plasmid Preparation, Electrophoresis, Marker, Construct