Review





Similar Products

brefeldin a  (InvivoGen)
94
InvivoGen brefeldin a
Brefeldin A, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bfa/pmc13208365-148-32-55?v=InvivoGen
Average 94 stars, based on 1 article reviews
brefeldin a - by Bioz Stars, 2026-07
94/100 stars
  Buy from Supplier
brefeldin a bfa  (MedChemExpress)
96
MedChemExpress brefeldin a bfa
Brefeldin A Bfa, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bfa/pm41966320-39-44-54?v=MedChemExpress
Average 96 stars, based on 1 article reviews
brefeldin a bfa - by Bioz Stars, 2026-07
96/100 stars
  Buy from Supplier
brefeldin a bfa treatment  (Thermo Fisher)
95
Thermo Fisher brefeldin a bfa treatment
KNOLLE as part of SNARE complex (E>>G:SNAP33/ big3 , estradiol-induced, +EST) either trapped at TGN (+ brefeldin A, <t>BFA)</t> or trafficked to the cell division plane (-BFA) was tested for ubiquitination. Lines for comparison: Con, non-transgenic control; G:KN, GFP-tagged KNOLLE.Protein extracts from seedlings treated with wortmannin (Wm) and NEM (+Wm/NEM) or untreated (-Wm/NEM) were immunoprecipitated with the GFP trap (IP(α-GFP)) and the enriched fractions probed with anti-GFP, anti-ubiquitin and anti-KNOLLE antibodies; bottom panel, prolonged exposure. Expected sizes: GFP:KNOLLE, 65 kDa; (UBQ)n-GFP:KNOLLE, 80-120 kDa (UBQ moiety 15-60 kDa); endogenous KNOLLE, 33 kDa; (UBQ)n-KNOLLE, 50-100 kDa. Brackets, ubiquitinated bands of GFP:KNOLLE; wide braces, range of (UBQ)n of KNOLLE between 50 and 100 kDa; narrow braces, the highest (UBQ)n band of KNOLLE at approximately 100 kDa. Asterisk, GFP:KNOLLE; double asterisks, GFP:SNAP33; hashtags, cross-reacting bands of anti-KNOLLE antiserum; numbers on the left, protein sizes in kDa.
Brefeldin A Bfa Treatment, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bfa/bio_rxiv__64898__2026__05__13__724867-140-3-15?v=Thermo+Fisher
Average 95 stars, based on 1 article reviews
brefeldin a bfa treatment - by Bioz Stars, 2026-07
95/100 stars
  Buy from Supplier
brefeldin a bfa  (Haoyuan Chemexpress Co Ltd)
86
Haoyuan Chemexpress Co Ltd brefeldin a bfa
KNOLLE as part of SNARE complex (E>>G:SNAP33/ big3 , estradiol-induced, +EST) either trapped at TGN (+ brefeldin A, <t>BFA)</t> or trafficked to the cell division plane (-BFA) was tested for ubiquitination. Lines for comparison: Con, non-transgenic control; G:KN, GFP-tagged KNOLLE.Protein extracts from seedlings treated with wortmannin (Wm) and NEM (+Wm/NEM) or untreated (-Wm/NEM) were immunoprecipitated with the GFP trap (IP(α-GFP)) and the enriched fractions probed with anti-GFP, anti-ubiquitin and anti-KNOLLE antibodies; bottom panel, prolonged exposure. Expected sizes: GFP:KNOLLE, 65 kDa; (UBQ)n-GFP:KNOLLE, 80-120 kDa (UBQ moiety 15-60 kDa); endogenous KNOLLE, 33 kDa; (UBQ)n-KNOLLE, 50-100 kDa. Brackets, ubiquitinated bands of GFP:KNOLLE; wide braces, range of (UBQ)n of KNOLLE between 50 and 100 kDa; narrow braces, the highest (UBQ)n band of KNOLLE at approximately 100 kDa. Asterisk, GFP:KNOLLE; double asterisks, GFP:SNAP33; hashtags, cross-reacting bands of anti-KNOLLE antiserum; numbers on the left, protein sizes in kDa.
Brefeldin A Bfa, supplied by Haoyuan Chemexpress Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bfa/pm42107845-80-69-81?v=Haoyuan+Chemexpress+Co+Ltd
Average 86 stars, based on 1 article reviews
brefeldin a bfa - by Bioz Stars, 2026-07
86/100 stars
  Buy from Supplier
bfa  (Selleck Chemicals)
95
Selleck Chemicals bfa
KNOLLE as part of SNARE complex (E>>G:SNAP33/ big3 , estradiol-induced, +EST) either trapped at TGN (+ brefeldin A, <t>BFA)</t> or trafficked to the cell division plane (-BFA) was tested for ubiquitination. Lines for comparison: Con, non-transgenic control; G:KN, GFP-tagged KNOLLE.Protein extracts from seedlings treated with wortmannin (Wm) and NEM (+Wm/NEM) or untreated (-Wm/NEM) were immunoprecipitated with the GFP trap (IP(α-GFP)) and the enriched fractions probed with anti-GFP, anti-ubiquitin and anti-KNOLLE antibodies; bottom panel, prolonged exposure. Expected sizes: GFP:KNOLLE, 65 kDa; (UBQ)n-GFP:KNOLLE, 80-120 kDa (UBQ moiety 15-60 kDa); endogenous KNOLLE, 33 kDa; (UBQ)n-KNOLLE, 50-100 kDa. Brackets, ubiquitinated bands of GFP:KNOLLE; wide braces, range of (UBQ)n of KNOLLE between 50 and 100 kDa; narrow braces, the highest (UBQ)n band of KNOLLE at approximately 100 kDa. Asterisk, GFP:KNOLLE; double asterisks, GFP:SNAP33; hashtags, cross-reacting bands of anti-KNOLLE antiserum; numbers on the left, protein sizes in kDa.
Bfa, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bfa/pm41896559-392-13-14?v=Selleck+Chemicals
Average 95 stars, based on 1 article reviews
bfa - by Bioz Stars, 2026-07
95/100 stars
  Buy from Supplier
bfa  (Merck & Co)
86
Merck & Co bfa
KNOLLE as part of SNARE complex (E>>G:SNAP33/ big3 , estradiol-induced, +EST) either trapped at TGN (+ brefeldin A, <t>BFA)</t> or trafficked to the cell division plane (-BFA) was tested for ubiquitination. Lines for comparison: Con, non-transgenic control; G:KN, GFP-tagged KNOLLE.Protein extracts from seedlings treated with wortmannin (Wm) and NEM (+Wm/NEM) or untreated (-Wm/NEM) were immunoprecipitated with the GFP trap (IP(α-GFP)) and the enriched fractions probed with anti-GFP, anti-ubiquitin and anti-KNOLLE antibodies; bottom panel, prolonged exposure. Expected sizes: GFP:KNOLLE, 65 kDa; (UBQ)n-GFP:KNOLLE, 80-120 kDa (UBQ moiety 15-60 kDa); endogenous KNOLLE, 33 kDa; (UBQ)n-KNOLLE, 50-100 kDa. Brackets, ubiquitinated bands of GFP:KNOLLE; wide braces, range of (UBQ)n of KNOLLE between 50 and 100 kDa; narrow braces, the highest (UBQ)n band of KNOLLE at approximately 100 kDa. Asterisk, GFP:KNOLLE; double asterisks, GFP:SNAP33; hashtags, cross-reacting bands of anti-KNOLLE antiserum; numbers on the left, protein sizes in kDa.
Bfa, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bfa/us12584920-165-0-4?v=Merck+%26+Co
Average 86 stars, based on 1 article reviews
bfa - by Bioz Stars, 2026-07
86/100 stars
  Buy from Supplier
protein transport inhibitor  (Multi Sciences (Lianke) Biotech Co Ltd)
95
Multi Sciences (Lianke) Biotech Co Ltd protein transport inhibitor
KNOLLE as part of SNARE complex (E>>G:SNAP33/ big3 , estradiol-induced, +EST) either trapped at TGN (+ brefeldin A, <t>BFA)</t> or trafficked to the cell division plane (-BFA) was tested for ubiquitination. Lines for comparison: Con, non-transgenic control; G:KN, GFP-tagged KNOLLE.Protein extracts from seedlings treated with wortmannin (Wm) and NEM (+Wm/NEM) or untreated (-Wm/NEM) were immunoprecipitated with the GFP trap (IP(α-GFP)) and the enriched fractions probed with anti-GFP, anti-ubiquitin and anti-KNOLLE antibodies; bottom panel, prolonged exposure. Expected sizes: GFP:KNOLLE, 65 kDa; (UBQ)n-GFP:KNOLLE, 80-120 kDa (UBQ moiety 15-60 kDa); endogenous KNOLLE, 33 kDa; (UBQ)n-KNOLLE, 50-100 kDa. Brackets, ubiquitinated bands of GFP:KNOLLE; wide braces, range of (UBQ)n of KNOLLE between 50 and 100 kDa; narrow braces, the highest (UBQ)n band of KNOLLE at approximately 100 kDa. Asterisk, GFP:KNOLLE; double asterisks, GFP:SNAP33; hashtags, cross-reacting bands of anti-KNOLLE antiserum; numbers on the left, protein sizes in kDa.
Protein Transport Inhibitor, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bfa/pm41839286-78-18-26?v=Multi+Sciences+%28Lianke%29+Biotech+Co+Ltd
Average 95 stars, based on 1 article reviews
protein transport inhibitor - by Bioz Stars, 2026-07
95/100 stars
  Buy from Supplier

Image Search Results


KNOLLE as part of SNARE complex (E>>G:SNAP33/ big3 , estradiol-induced, +EST) either trapped at TGN (+ brefeldin A, BFA) or trafficked to the cell division plane (-BFA) was tested for ubiquitination. Lines for comparison: Con, non-transgenic control; G:KN, GFP-tagged KNOLLE.Protein extracts from seedlings treated with wortmannin (Wm) and NEM (+Wm/NEM) or untreated (-Wm/NEM) were immunoprecipitated with the GFP trap (IP(α-GFP)) and the enriched fractions probed with anti-GFP, anti-ubiquitin and anti-KNOLLE antibodies; bottom panel, prolonged exposure. Expected sizes: GFP:KNOLLE, 65 kDa; (UBQ)n-GFP:KNOLLE, 80-120 kDa (UBQ moiety 15-60 kDa); endogenous KNOLLE, 33 kDa; (UBQ)n-KNOLLE, 50-100 kDa. Brackets, ubiquitinated bands of GFP:KNOLLE; wide braces, range of (UBQ)n of KNOLLE between 50 and 100 kDa; narrow braces, the highest (UBQ)n band of KNOLLE at approximately 100 kDa. Asterisk, GFP:KNOLLE; double asterisks, GFP:SNAP33; hashtags, cross-reacting bands of anti-KNOLLE antiserum; numbers on the left, protein sizes in kDa.

Journal: bioRxiv

Article Title: Degradation of cytokinesis-specific Qa-SNARE KNOLLE is regulated by context-dependent ubiquitination

doi: 10.64898/2026.05.13.724867

Figure Lengend Snippet: KNOLLE as part of SNARE complex (E>>G:SNAP33/ big3 , estradiol-induced, +EST) either trapped at TGN (+ brefeldin A, BFA) or trafficked to the cell division plane (-BFA) was tested for ubiquitination. Lines for comparison: Con, non-transgenic control; G:KN, GFP-tagged KNOLLE.Protein extracts from seedlings treated with wortmannin (Wm) and NEM (+Wm/NEM) or untreated (-Wm/NEM) were immunoprecipitated with the GFP trap (IP(α-GFP)) and the enriched fractions probed with anti-GFP, anti-ubiquitin and anti-KNOLLE antibodies; bottom panel, prolonged exposure. Expected sizes: GFP:KNOLLE, 65 kDa; (UBQ)n-GFP:KNOLLE, 80-120 kDa (UBQ moiety 15-60 kDa); endogenous KNOLLE, 33 kDa; (UBQ)n-KNOLLE, 50-100 kDa. Brackets, ubiquitinated bands of GFP:KNOLLE; wide braces, range of (UBQ)n of KNOLLE between 50 and 100 kDa; narrow braces, the highest (UBQ)n band of KNOLLE at approximately 100 kDa. Asterisk, GFP:KNOLLE; double asterisks, GFP:SNAP33; hashtags, cross-reacting bands of anti-KNOLLE antiserum; numbers on the left, protein sizes in kDa.

Article Snippet: Estradiol induction and brefeldin A (BFA) treatment: 50 μM BFA (50 mM stock in DMSO/Ethanol, Invitrogen) was applied for 30 minutes, followed by 20 μM estradiol (20 mM stock in DMSO, Sigma-aldrich) for 3 hours and 30 minutes in the presence of BFA and subsequently wortmannin and NEM were added as described above.

Techniques: Ubiquitin Proteomics, Comparison, Transgenic Assay, Control, Immunoprecipitation