Journal: iScience

Article Title: Macrophage AMPK β1 activation by PF-06409577 reduces the inflammatory response, cholesterol synthesis, and atherosclerosis in mice

doi: 10.1016/j.isci.2023.108269

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Article Snippet: beta-Actin (13E5) Rabbit mAb (HRP Conjugate , Cell Signaling Technology , Cat# 5125; RRID: AB_1903890.

Techniques: Virus, Plasmid Preparation, Recombinant, Colorimetric Assay, Enzyme-linked Immunosorbent Assay, RNA Sequencing Assay, Western Blot, Software

Journal: iScience

Article Title: hGFAP -mediated GLI2 overexpression leads to early death and severe cerebellar malformations with rare tumor formation

doi: 10.1016/j.isci.2023.107501

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Article Snippet: Rabbit monoclonal anti-ß Actin , Cell Signaling Technology , at# 8457; RRID: AB_10950489.

Techniques: Clone Assay, Recombinant, Plasmid Preparation, Software, Imaging

Journal: Journal of Cellular and Molecular Medicine

Article Title: GP ‐2250, a novel anticancer agent, inhibits the energy metabolism, activates AMP‐Kinase and impairs the NF‐kB pathway in pancreatic cancer cells

doi: 10.1111/jcmm.17825

Figure Lengend Snippet: Representative western blot (A) and quantitative analysis (B) of pAMPK and cyclin D1. pAMPK: Time dependency of AMPK phosphorylation at T172 following incubation of Panc Tul with GP‐2250 (250 μM).) Dose‐dependency of AMPK phosphorylation after incubation of BxPC3 cells+ for 24 h. Cyclin D1: Time‐dependent decrease of cyclin D1 following incubation of Panc Tul (500 μM GP‐2250) and BxPC3 cells (250 μM GP‐2250). ß‐Actin/HSP‐90 used as internal controls. NC, negative control.

Article Snippet: Thereafter, the membranes were blocked in EveryBlot Blocking Buffer (BIO RAD) according to the manufacturer's antibody specification protocol for 5 min and incubated overnight at 4°C with the respective primary antibody [P‐AMPKalpha Rabbit mAB (T172) #2535, P‐ACC (Ser79) Rabbit mAB #11818, P‐Raptor (Ser792) Rabbit mAB #2083, p53 Rabbit mAB #2527, mTOR Rabbit Ab #2972, Akt Rabbit Ab #9272, cyclin D1 Rabbit mAB #55506, beta‐actin Rabbit mAB #8457, α‐tubulin Rabbit mAb #2125, Bcl2 Rabbit mAb #4223 and BAX Rabbit AB #2772 (CST)] at 1:1000 dilution.

Techniques: Western Blot, Incubation, Negative Control

Journal: Journal of Cellular and Molecular Medicine

Article Title: GP ‐2250, a novel anticancer agent, inhibits the energy metabolism, activates AMP‐Kinase and impairs the NF‐kB pathway in pancreatic cancer cells

doi: 10.1111/jcmm.17825

Figure Lengend Snippet: Representative western blot (A) and quantitative analysis (B) of regulatory proteins in Panc TuI (A) and BxPC3 (B) cells. pACC: Time‐dependent increase of ACC‐1 phosphorylation at Ser79 and of Raptor at Ser792 following incubation of Panc Tul (250 μM GP‐2250) and BxPC3 (500 μM GP‐2250). pRaptor: Time‐dependent Raptor phosphorylation at Ser 792 in PancTul and BxPc3 cells incubated with 500 and 1000 μM GP‐2250. p53: Time‐dependent increase of protein level of p53 in Panc Tul and BxPC3 cells (500 μM GP‐2250). Akt and mTor: Time‐dependent decrease of the protein level of Akt and mTor at 1000 μM GP‐2250 in Panc TuI and BxPC3 cells. Bcl2: Time‐dependent downregulation of protein level of Bcl2 following incubation of Panc Tul and BxPC3 cells (500 μM GP‐2250). ß‐Actin/HSP‐90 used as internal controls.

Article Snippet: Thereafter, the membranes were blocked in EveryBlot Blocking Buffer (BIO RAD) according to the manufacturer's antibody specification protocol for 5 min and incubated overnight at 4°C with the respective primary antibody [P‐AMPKalpha Rabbit mAB (T172) #2535, P‐ACC (Ser79) Rabbit mAB #11818, P‐Raptor (Ser792) Rabbit mAB #2083, p53 Rabbit mAB #2527, mTOR Rabbit Ab #2972, Akt Rabbit Ab #9272, cyclin D1 Rabbit mAB #55506, beta‐actin Rabbit mAB #8457, α‐tubulin Rabbit mAb #2125, Bcl2 Rabbit mAb #4223 and BAX Rabbit AB #2772 (CST)] at 1:1000 dilution.

Techniques: Western Blot, Incubation