beta cell mass morphometry  (Millipore)


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    Millipore beta cell mass morphometry
    <t>Beta</t> cell adaptation in islets grafts from different pancreatic regions transplanted in syngeneic diabetic mice. A. Average islet size per transplant. B. Blood glucose concentrations of STZ-induced diabetic mice followed up to 10 days after transplantation (n = 6–8 mice per region) of DR, GR or SR islets. C. AUC blood glucose concentrations post-transplantation corrected for pre-transplantation glucose concentration (n = 6–8 mice per region). D. Image of proliferating beta-cells, positive for both BrdU (brown) and insulin (red) in islets transplanted under the kidney capsule of diabetic mice. Scale bar = 20 µm. E. Beta cell proliferation in the islet grafts 10 days after transplantation, BrdU labeling during the final 7 days (n = 6–7 mice per region). DR = duodenal region, GR = gastric region, SR = splenic region, AUC = area under the curve.
    Beta Cell Mass Morphometry, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/beta cell mass morphometry/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    beta cell mass morphometry - by Bioz Stars, 2020-08
    85/100 stars

    Related Products / Commonly Used Together

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    guinea-pig anti-insulin igg

    Images

    1) Product Images from "Topologically Heterogeneous Beta Cell Adaptation in Response to High-Fat Diet in Mice"

    Article Title: Topologically Heterogeneous Beta Cell Adaptation in Response to High-Fat Diet in Mice

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0056922

    Beta cell adaptation in islets grafts from different pancreatic regions transplanted in syngeneic diabetic mice. A. Average islet size per transplant. B. Blood glucose concentrations of STZ-induced diabetic mice followed up to 10 days after transplantation (n = 6–8 mice per region) of DR, GR or SR islets. C. AUC blood glucose concentrations post-transplantation corrected for pre-transplantation glucose concentration (n = 6–8 mice per region). D. Image of proliferating beta-cells, positive for both BrdU (brown) and insulin (red) in islets transplanted under the kidney capsule of diabetic mice. Scale bar = 20 µm. E. Beta cell proliferation in the islet grafts 10 days after transplantation, BrdU labeling during the final 7 days (n = 6–7 mice per region). DR = duodenal region, GR = gastric region, SR = splenic region, AUC = area under the curve.
    Figure Legend Snippet: Beta cell adaptation in islets grafts from different pancreatic regions transplanted in syngeneic diabetic mice. A. Average islet size per transplant. B. Blood glucose concentrations of STZ-induced diabetic mice followed up to 10 days after transplantation (n = 6–8 mice per region) of DR, GR or SR islets. C. AUC blood glucose concentrations post-transplantation corrected for pre-transplantation glucose concentration (n = 6–8 mice per region). D. Image of proliferating beta-cells, positive for both BrdU (brown) and insulin (red) in islets transplanted under the kidney capsule of diabetic mice. Scale bar = 20 µm. E. Beta cell proliferation in the islet grafts 10 days after transplantation, BrdU labeling during the final 7 days (n = 6–7 mice per region). DR = duodenal region, GR = gastric region, SR = splenic region, AUC = area under the curve.

    Techniques Used: Mouse Assay, Transplantation Assay, Concentration Assay, Labeling

    Beta cell mass morphometry in control and HFD mice. A. Beta cell mass in the entire pancreas after 6 weeks (n = 6 mice). B. Beta cell mass by pancreatic region after 6 weeks (n = 6 mice per region). C. Beta cell cluster area in the entire pancreas after 6 weeks (n = 6 mice). D. Beta cell cluster area by pancreatic region after 6 weeks (n = 6 mice per region). E. Islet density in the entire pancreas after 6 weeks (n = 6 mice). F. Islet density by pancreatic region after 6 weeks (n = 6 mice per region). G. Beta cell area in the entire pancreas after 12 weeks (n = 6 mice). H. Beta cell area by pancreatic region after 12 weeks (n = 6 mice per region). DR = duodenal region, GR = gastric region, SR = splenic region, HFD = high-fat diet. * p
    Figure Legend Snippet: Beta cell mass morphometry in control and HFD mice. A. Beta cell mass in the entire pancreas after 6 weeks (n = 6 mice). B. Beta cell mass by pancreatic region after 6 weeks (n = 6 mice per region). C. Beta cell cluster area in the entire pancreas after 6 weeks (n = 6 mice). D. Beta cell cluster area by pancreatic region after 6 weeks (n = 6 mice per region). E. Islet density in the entire pancreas after 6 weeks (n = 6 mice). F. Islet density by pancreatic region after 6 weeks (n = 6 mice per region). G. Beta cell area in the entire pancreas after 12 weeks (n = 6 mice). H. Beta cell area by pancreatic region after 12 weeks (n = 6 mice per region). DR = duodenal region, GR = gastric region, SR = splenic region, HFD = high-fat diet. * p

    Techniques Used: Mouse Assay

    Beta cell proliferation in control and HFD mice after 6 weeks. A. Image of proliferating beta-cells (arrowheads), Ki67 (green), insulin (red) and DAPI (blue). Scale bar = 50 µm. B. Image of proliferating beta-cells (arrowheads), BrdU (brown) and insulin (red) per pancreatic region in control and HFD mice. Mice received BrdU during the final 7 days. Scale bar = 50 µm. C. Beta cell proliferation in the entire pancreas, BrdU labeling during the final 7 days (n = 6 mice). D. Beta cell proliferation by pancreatic region, BrdU labeling during the final 7 days (n = 6 mice per region). E. Cyclin D1 mRNA expression by pancreatic region, control = 1. DR = duodenal region, GR = gastric region, SR = splenic region, HFD = high-fat diet. * p
    Figure Legend Snippet: Beta cell proliferation in control and HFD mice after 6 weeks. A. Image of proliferating beta-cells (arrowheads), Ki67 (green), insulin (red) and DAPI (blue). Scale bar = 50 µm. B. Image of proliferating beta-cells (arrowheads), BrdU (brown) and insulin (red) per pancreatic region in control and HFD mice. Mice received BrdU during the final 7 days. Scale bar = 50 µm. C. Beta cell proliferation in the entire pancreas, BrdU labeling during the final 7 days (n = 6 mice). D. Beta cell proliferation by pancreatic region, BrdU labeling during the final 7 days (n = 6 mice per region). E. Cyclin D1 mRNA expression by pancreatic region, control = 1. DR = duodenal region, GR = gastric region, SR = splenic region, HFD = high-fat diet. * p

    Techniques Used: Mouse Assay, Labeling, Expressing

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    Millipore beta cell mass morphometry
    <t>Beta</t> cell adaptation in islets grafts from different pancreatic regions transplanted in syngeneic diabetic mice. A. Average islet size per transplant. B. Blood glucose concentrations of STZ-induced diabetic mice followed up to 10 days after transplantation (n = 6–8 mice per region) of DR, GR or SR islets. C. AUC blood glucose concentrations post-transplantation corrected for pre-transplantation glucose concentration (n = 6–8 mice per region). D. Image of proliferating beta-cells, positive for both BrdU (brown) and insulin (red) in islets transplanted under the kidney capsule of diabetic mice. Scale bar = 20 µm. E. Beta cell proliferation in the islet grafts 10 days after transplantation, BrdU labeling during the final 7 days (n = 6–7 mice per region). DR = duodenal region, GR = gastric region, SR = splenic region, AUC = area under the curve.
    Beta Cell Mass Morphometry, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/beta cell mass morphometry/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    beta cell mass morphometry - by Bioz Stars, 2020-08
    85/100 stars
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    Beta cell adaptation in islets grafts from different pancreatic regions transplanted in syngeneic diabetic mice. A. Average islet size per transplant. B. Blood glucose concentrations of STZ-induced diabetic mice followed up to 10 days after transplantation (n = 6–8 mice per region) of DR, GR or SR islets. C. AUC blood glucose concentrations post-transplantation corrected for pre-transplantation glucose concentration (n = 6–8 mice per region). D. Image of proliferating beta-cells, positive for both BrdU (brown) and insulin (red) in islets transplanted under the kidney capsule of diabetic mice. Scale bar = 20 µm. E. Beta cell proliferation in the islet grafts 10 days after transplantation, BrdU labeling during the final 7 days (n = 6–7 mice per region). DR = duodenal region, GR = gastric region, SR = splenic region, AUC = area under the curve.

    Journal: PLoS ONE

    Article Title: Topologically Heterogeneous Beta Cell Adaptation in Response to High-Fat Diet in Mice

    doi: 10.1371/journal.pone.0056922

    Figure Lengend Snippet: Beta cell adaptation in islets grafts from different pancreatic regions transplanted in syngeneic diabetic mice. A. Average islet size per transplant. B. Blood glucose concentrations of STZ-induced diabetic mice followed up to 10 days after transplantation (n = 6–8 mice per region) of DR, GR or SR islets. C. AUC blood glucose concentrations post-transplantation corrected for pre-transplantation glucose concentration (n = 6–8 mice per region). D. Image of proliferating beta-cells, positive for both BrdU (brown) and insulin (red) in islets transplanted under the kidney capsule of diabetic mice. Scale bar = 20 µm. E. Beta cell proliferation in the islet grafts 10 days after transplantation, BrdU labeling during the final 7 days (n = 6–7 mice per region). DR = duodenal region, GR = gastric region, SR = splenic region, AUC = area under the curve.

    Article Snippet: Beta cell mass morphometry and proliferation For the identification of beta cells, sections were immunostained with guinea-pig anti-insulin IgG (Millipore, Billerica, MA, USA) or rabbit anti-insulin IgG (Santa Cruz Biotechnology, Santa Cruz, CA, USA) for 1 hour followed by HRP- or AP- conjugated secondary antibodies for 1 hour.

    Techniques: Mouse Assay, Transplantation Assay, Concentration Assay, Labeling

    Beta cell mass morphometry in control and HFD mice. A. Beta cell mass in the entire pancreas after 6 weeks (n = 6 mice). B. Beta cell mass by pancreatic region after 6 weeks (n = 6 mice per region). C. Beta cell cluster area in the entire pancreas after 6 weeks (n = 6 mice). D. Beta cell cluster area by pancreatic region after 6 weeks (n = 6 mice per region). E. Islet density in the entire pancreas after 6 weeks (n = 6 mice). F. Islet density by pancreatic region after 6 weeks (n = 6 mice per region). G. Beta cell area in the entire pancreas after 12 weeks (n = 6 mice). H. Beta cell area by pancreatic region after 12 weeks (n = 6 mice per region). DR = duodenal region, GR = gastric region, SR = splenic region, HFD = high-fat diet. * p

    Journal: PLoS ONE

    Article Title: Topologically Heterogeneous Beta Cell Adaptation in Response to High-Fat Diet in Mice

    doi: 10.1371/journal.pone.0056922

    Figure Lengend Snippet: Beta cell mass morphometry in control and HFD mice. A. Beta cell mass in the entire pancreas after 6 weeks (n = 6 mice). B. Beta cell mass by pancreatic region after 6 weeks (n = 6 mice per region). C. Beta cell cluster area in the entire pancreas after 6 weeks (n = 6 mice). D. Beta cell cluster area by pancreatic region after 6 weeks (n = 6 mice per region). E. Islet density in the entire pancreas after 6 weeks (n = 6 mice). F. Islet density by pancreatic region after 6 weeks (n = 6 mice per region). G. Beta cell area in the entire pancreas after 12 weeks (n = 6 mice). H. Beta cell area by pancreatic region after 12 weeks (n = 6 mice per region). DR = duodenal region, GR = gastric region, SR = splenic region, HFD = high-fat diet. * p

    Article Snippet: Beta cell mass morphometry and proliferation For the identification of beta cells, sections were immunostained with guinea-pig anti-insulin IgG (Millipore, Billerica, MA, USA) or rabbit anti-insulin IgG (Santa Cruz Biotechnology, Santa Cruz, CA, USA) for 1 hour followed by HRP- or AP- conjugated secondary antibodies for 1 hour.

    Techniques: Mouse Assay

    Beta cell proliferation in control and HFD mice after 6 weeks. A. Image of proliferating beta-cells (arrowheads), Ki67 (green), insulin (red) and DAPI (blue). Scale bar = 50 µm. B. Image of proliferating beta-cells (arrowheads), BrdU (brown) and insulin (red) per pancreatic region in control and HFD mice. Mice received BrdU during the final 7 days. Scale bar = 50 µm. C. Beta cell proliferation in the entire pancreas, BrdU labeling during the final 7 days (n = 6 mice). D. Beta cell proliferation by pancreatic region, BrdU labeling during the final 7 days (n = 6 mice per region). E. Cyclin D1 mRNA expression by pancreatic region, control = 1. DR = duodenal region, GR = gastric region, SR = splenic region, HFD = high-fat diet. * p

    Journal: PLoS ONE

    Article Title: Topologically Heterogeneous Beta Cell Adaptation in Response to High-Fat Diet in Mice

    doi: 10.1371/journal.pone.0056922

    Figure Lengend Snippet: Beta cell proliferation in control and HFD mice after 6 weeks. A. Image of proliferating beta-cells (arrowheads), Ki67 (green), insulin (red) and DAPI (blue). Scale bar = 50 µm. B. Image of proliferating beta-cells (arrowheads), BrdU (brown) and insulin (red) per pancreatic region in control and HFD mice. Mice received BrdU during the final 7 days. Scale bar = 50 µm. C. Beta cell proliferation in the entire pancreas, BrdU labeling during the final 7 days (n = 6 mice). D. Beta cell proliferation by pancreatic region, BrdU labeling during the final 7 days (n = 6 mice per region). E. Cyclin D1 mRNA expression by pancreatic region, control = 1. DR = duodenal region, GR = gastric region, SR = splenic region, HFD = high-fat diet. * p

    Article Snippet: Beta cell mass morphometry and proliferation For the identification of beta cells, sections were immunostained with guinea-pig anti-insulin IgG (Millipore, Billerica, MA, USA) or rabbit anti-insulin IgG (Santa Cruz Biotechnology, Santa Cruz, CA, USA) for 1 hour followed by HRP- or AP- conjugated secondary antibodies for 1 hour.

    Techniques: Mouse Assay, Labeling, Expressing