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Enzo Biochem beta β mhc
Characterization of hiPSCs and hiPSC-derived CMs. (A) Immunofluorescent staining of hiPSC colonies with antibodies against Oct-4, SSEA-4, TRA-1-60 and TRA-1-81. (B) The hiPSC-CMs differentia4ed from above hiPSC line. (Ba) The phase-contrast light micrograph images of a V-CM cluster. (Bb and Bc) Immunofluorescent staining hiPSC-CMs with antibodies against alpha-actinin and <t>beta-MHC,</t> respectively. Nuclei were stained with DAPI. (C) Action potential traces of ventricular-, atrial- and nodal-like CMs derived from hiPSCs. (D) Response of a ventricular-like hiPSC-CM to ISO recorded with patch-clamp. Abbreviations: ISO, isoproterenol.
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1) Product Images from "Identification and Characterization of Calcium Sparks in Cardiomyocytes Derived from Human Induced Pluripotent Stem Cells"

Article Title: Identification and Characterization of Calcium Sparks in Cardiomyocytes Derived from Human Induced Pluripotent Stem Cells

Journal: PLoS ONE

doi: 10.1371/journal.pone.0055266

Characterization of hiPSCs and hiPSC-derived CMs. (A) Immunofluorescent staining of hiPSC colonies with antibodies against Oct-4, SSEA-4, TRA-1-60 and TRA-1-81. (B) The hiPSC-CMs differentia4ed from above hiPSC line. (Ba) The phase-contrast light micrograph images of a V-CM cluster. (Bb and Bc) Immunofluorescent staining hiPSC-CMs with antibodies against alpha-actinin and beta-MHC, respectively. Nuclei were stained with DAPI. (C) Action potential traces of ventricular-, atrial- and nodal-like CMs derived from hiPSCs. (D) Response of a ventricular-like hiPSC-CM to ISO recorded with patch-clamp. Abbreviations: ISO, isoproterenol.
Figure Legend Snippet: Characterization of hiPSCs and hiPSC-derived CMs. (A) Immunofluorescent staining of hiPSC colonies with antibodies against Oct-4, SSEA-4, TRA-1-60 and TRA-1-81. (B) The hiPSC-CMs differentia4ed from above hiPSC line. (Ba) The phase-contrast light micrograph images of a V-CM cluster. (Bb and Bc) Immunofluorescent staining hiPSC-CMs with antibodies against alpha-actinin and beta-MHC, respectively. Nuclei were stained with DAPI. (C) Action potential traces of ventricular-, atrial- and nodal-like CMs derived from hiPSCs. (D) Response of a ventricular-like hiPSC-CM to ISO recorded with patch-clamp. Abbreviations: ISO, isoproterenol.

Techniques Used: Derivative Assay, Staining, Patch Clamp

Related Articles

Staining:

Article Title: Identification and Characterization of Calcium Sparks in Cardiomyocytes Derived from Human Induced Pluripotent Stem Cells
Article Snippet: .. After blocking with 5% goat serum in PBS for 1 h at room temperature, cells stained with mouse anti-human cardiac sarcomeric alpha-actinin (α-actinin) (clone EA-35, Sigma) and mouse anti-human cardiac myosin heave chain, beta (β-MHC) (Alexis Biochemicals, FL, USA). .. Next, the primary mAbs was removed and replaced with goat anti–mouse IgG (A11001 Alexa Fluor 488, Invitrogen) for 1 hour.

Blocking Assay:

Article Title: Identification and Characterization of Calcium Sparks in Cardiomyocytes Derived from Human Induced Pluripotent Stem Cells
Article Snippet: .. After blocking with 5% goat serum in PBS for 1 h at room temperature, cells stained with mouse anti-human cardiac sarcomeric alpha-actinin (α-actinin) (clone EA-35, Sigma) and mouse anti-human cardiac myosin heave chain, beta (β-MHC) (Alexis Biochemicals, FL, USA). .. Next, the primary mAbs was removed and replaced with goat anti–mouse IgG (A11001 Alexa Fluor 488, Invitrogen) for 1 hour.