anti bestrophin 1 extracellular antibody  (Alomone Labs)


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    Structured Review

    Alomone Labs anti bestrophin 1 extracellular antibody
    <t>Bestrophin-1</t> is expressed in the spinal cord and DRG. RT-PCR ( a , b ) and western blot ( c , d ) analysis of bestrophin-1 <t>(Best-1)</t> at the ipsilateral dorsal portion of the spinal cord (SC) and DRG obtained from naïve (Na), sham (S) and spinal nerve ligated (SNL) rats. Insets show a representative band of the PCR product ( upper panels ) and immunoblot ( lower panels ) obtained with the specific bestrophin-1 and β-actin primers or <t>antibodies,</t> respectively. Data were normalized against β-actin and are expressed as the mean ± SEM of three independent rats. Note that spinal nerve injury did not modify Best-1 mRNA and protein expression.
    Anti Bestrophin 1 Extracellular Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti bestrophin 1 extracellular antibody/product/Alomone Labs
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    anti bestrophin 1 extracellular antibody - by Bioz Stars, 2022-11
    85/100 stars

    Images

    1) Product Images from "Role of anoctamin-1 and bestrophin-1 in spinal nerve ligation-induced neuropathic pain in rats"

    Article Title: Role of anoctamin-1 and bestrophin-1 in spinal nerve ligation-induced neuropathic pain in rats

    Journal: Molecular Pain

    doi: 10.1186/s12990-015-0042-1

    Bestrophin-1 is expressed in the spinal cord and DRG. RT-PCR ( a , b ) and western blot ( c , d ) analysis of bestrophin-1 (Best-1) at the ipsilateral dorsal portion of the spinal cord (SC) and DRG obtained from naïve (Na), sham (S) and spinal nerve ligated (SNL) rats. Insets show a representative band of the PCR product ( upper panels ) and immunoblot ( lower panels ) obtained with the specific bestrophin-1 and β-actin primers or antibodies, respectively. Data were normalized against β-actin and are expressed as the mean ± SEM of three independent rats. Note that spinal nerve injury did not modify Best-1 mRNA and protein expression.
    Figure Legend Snippet: Bestrophin-1 is expressed in the spinal cord and DRG. RT-PCR ( a , b ) and western blot ( c , d ) analysis of bestrophin-1 (Best-1) at the ipsilateral dorsal portion of the spinal cord (SC) and DRG obtained from naïve (Na), sham (S) and spinal nerve ligated (SNL) rats. Insets show a representative band of the PCR product ( upper panels ) and immunoblot ( lower panels ) obtained with the specific bestrophin-1 and β-actin primers or antibodies, respectively. Data were normalized against β-actin and are expressed as the mean ± SEM of three independent rats. Note that spinal nerve injury did not modify Best-1 mRNA and protein expression.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Western Blot, Polymerase Chain Reaction, Expressing

    Intrathecal administration of selective antibody anti-bestrophin-1 or anti-anoctamin-1 reverses established allodynia and reduces protein expression. Time-course of the antiallodynic effect of antibodies against bestrophin-1 (Best-1 ab, 2 μg/6 h × 30 h; a ) or anoctamin-1 (Ano-1 ab, 2 μg/6 h x 30 h; d ) in rats subjected to L5/L6 spinal nerve ligation. Paw withdrawal threshold was assessed every 6 h starting 12 days after spinal nerve ligation. Data are presented as the mean ± SEM for six animals. *Significantly different from the vehicle group (p
    Figure Legend Snippet: Intrathecal administration of selective antibody anti-bestrophin-1 or anti-anoctamin-1 reverses established allodynia and reduces protein expression. Time-course of the antiallodynic effect of antibodies against bestrophin-1 (Best-1 ab, 2 μg/6 h × 30 h; a ) or anoctamin-1 (Ano-1 ab, 2 μg/6 h x 30 h; d ) in rats subjected to L5/L6 spinal nerve ligation. Paw withdrawal threshold was assessed every 6 h starting 12 days after spinal nerve ligation. Data are presented as the mean ± SEM for six animals. *Significantly different from the vehicle group (p

    Techniques Used: Expressing, Ligation

    2) Product Images from "Role of anoctamin-1 and bestrophin-1 in spinal nerve ligation-induced neuropathic pain in rats"

    Article Title: Role of anoctamin-1 and bestrophin-1 in spinal nerve ligation-induced neuropathic pain in rats

    Journal: Molecular Pain

    doi: 10.1186/s12990-015-0042-1

    Bestrophin-1 is expressed in the spinal cord and DRG. RT-PCR ( a , b ) and western blot ( c , d ) analysis of bestrophin-1 (Best-1) at the ipsilateral dorsal portion of the spinal cord (SC) and DRG obtained from naïve (Na), sham (S) and spinal nerve ligated (SNL) rats. Insets show a representative band of the PCR product ( upper panels ) and immunoblot ( lower panels ) obtained with the specific bestrophin-1 and β-actin primers or antibodies, respectively. Data were normalized against β-actin and are expressed as the mean ± SEM of three independent rats. Note that spinal nerve injury did not modify Best-1 mRNA and protein expression.
    Figure Legend Snippet: Bestrophin-1 is expressed in the spinal cord and DRG. RT-PCR ( a , b ) and western blot ( c , d ) analysis of bestrophin-1 (Best-1) at the ipsilateral dorsal portion of the spinal cord (SC) and DRG obtained from naïve (Na), sham (S) and spinal nerve ligated (SNL) rats. Insets show a representative band of the PCR product ( upper panels ) and immunoblot ( lower panels ) obtained with the specific bestrophin-1 and β-actin primers or antibodies, respectively. Data were normalized against β-actin and are expressed as the mean ± SEM of three independent rats. Note that spinal nerve injury did not modify Best-1 mRNA and protein expression.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Western Blot, Polymerase Chain Reaction, Expressing

    Intrathecal administration of selective antibody anti-bestrophin-1 or anti-anoctamin-1 reverses established allodynia and reduces protein expression. Time-course of the antiallodynic effect of antibodies against bestrophin-1 (Best-1 ab, 2 μg/6 h × 30 h; a ) or anoctamin-1 (Ano-1 ab, 2 μg/6 h x 30 h; d ) in rats subjected to L5/L6 spinal nerve ligation. Paw withdrawal threshold was assessed every 6 h starting 12 days after spinal nerve ligation. Data are presented as the mean ± SEM for six animals. *Significantly different from the vehicle group (p
    Figure Legend Snippet: Intrathecal administration of selective antibody anti-bestrophin-1 or anti-anoctamin-1 reverses established allodynia and reduces protein expression. Time-course of the antiallodynic effect of antibodies against bestrophin-1 (Best-1 ab, 2 μg/6 h × 30 h; a ) or anoctamin-1 (Ano-1 ab, 2 μg/6 h x 30 h; d ) in rats subjected to L5/L6 spinal nerve ligation. Paw withdrawal threshold was assessed every 6 h starting 12 days after spinal nerve ligation. Data are presented as the mean ± SEM for six animals. *Significantly different from the vehicle group (p

    Techniques Used: Expressing, Ligation

    3) Product Images from "Chloride channels mediate sodium sulphide-induced relaxation in rat uteri"

    Article Title: Chloride channels mediate sodium sulphide-induced relaxation in rat uteri

    Journal: British Journal of Pharmacology

    doi: 10.1111/bph.13161

    (A) RT-PCR data showing quantitative evidence of BEST-1 in rat uterus in oestrus compared with endogenous controls (GAPDH, actin, cyclin A and 18S). (B) Representative Western blot demonstrating bands (four different animals) of expected molecular mass
    Figure Legend Snippet: (A) RT-PCR data showing quantitative evidence of BEST-1 in rat uterus in oestrus compared with endogenous controls (GAPDH, actin, cyclin A and 18S). (B) Representative Western blot demonstrating bands (four different animals) of expected molecular mass

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Western Blot

    4) Product Images from "Chloride channels mediate sodium sulphide-induced relaxation in rat uteri"

    Article Title: Chloride channels mediate sodium sulphide-induced relaxation in rat uteri

    Journal: British Journal of Pharmacology

    doi: 10.1111/bph.13161

    (A) RT-PCR data showing quantitative evidence of BEST-1 in rat uterus in oestrus compared with endogenous controls (GAPDH, actin, cyclin A and 18S). (B) Representative Western blot demonstrating bands (four different animals) of expected molecular mass
    Figure Legend Snippet: (A) RT-PCR data showing quantitative evidence of BEST-1 in rat uterus in oestrus compared with endogenous controls (GAPDH, actin, cyclin A and 18S). (B) Representative Western blot demonstrating bands (four different animals) of expected molecular mass

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Western Blot

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    Alomone Labs bestrophin 2
    Effect of dextran sulfate sodium (DSS)-colitis on Cl − channel protein expression in murine colon. A : representative images of protein expression of the different characterized Cl − channels from epithelial lysates. BEST2, <t>bestrophin-2.</t> B : transmembrane protein 16A (TMEM16A) protein expression normalized to β-actin for quantification of control and DSS-colitis groups. TMEM16A expression (closed bar) is significantly decreased in DSS-colitis mice as compared with control. C and D : summarized data of Western blot quantification of BEST2 and CFTR normalized to β-actin in control and DSS-colitis mice. Neither protein was significantly altered from the control cohort. Bar graphs of summarized data represent means ± SE of 5 different animals from each respective group. * P
    Bestrophin 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bestrophin 2/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bestrophin 2 - by Bioz Stars, 2022-11
    90/100 stars
      Buy from Supplier

    85
    Alomone Labs anti bestrophin 1 extracellular antibody
    <t>Bestrophin-1</t> is expressed in the spinal cord and DRG. RT-PCR ( a , b ) and western blot ( c , d ) analysis of bestrophin-1 <t>(Best-1)</t> at the ipsilateral dorsal portion of the spinal cord (SC) and DRG obtained from naïve (Na), sham (S) and spinal nerve ligated (SNL) rats. Insets show a representative band of the PCR product ( upper panels ) and immunoblot ( lower panels ) obtained with the specific bestrophin-1 and β-actin primers or <t>antibodies,</t> respectively. Data were normalized against β-actin and are expressed as the mean ± SEM of three independent rats. Note that spinal nerve injury did not modify Best-1 mRNA and protein expression.
    Anti Bestrophin 1 Extracellular Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti bestrophin 1 extracellular antibody/product/Alomone Labs
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    anti bestrophin 1 extracellular antibody - by Bioz Stars, 2022-11
    85/100 stars
      Buy from Supplier

    85
    Alomone Labs control bestrophin 1
    <t>Bestrophin-1</t> is expressed in the spinal cord and DRG. RT-PCR ( a , b ) and western blot ( c , d ) analysis of bestrophin-1 <t>(Best-1)</t> at the ipsilateral dorsal portion of the spinal cord (SC) and DRG obtained from naïve (Na), sham (S) and spinal nerve ligated (SNL) rats. Insets show a representative band of the PCR product ( upper panels ) and immunoblot ( lower panels ) obtained with the specific bestrophin-1 and β-actin primers or antibodies, respectively. Data were normalized against β-actin and are expressed as the mean ± SEM of three independent rats. Note that spinal nerve injury did not modify Best-1 mRNA and protein expression.
    Control Bestrophin 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/control bestrophin 1/product/Alomone Labs
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    control bestrophin 1 - by Bioz Stars, 2022-11
    85/100 stars
      Buy from Supplier

    Image Search Results


    Effect of dextran sulfate sodium (DSS)-colitis on Cl − channel protein expression in murine colon. A : representative images of protein expression of the different characterized Cl − channels from epithelial lysates. BEST2, bestrophin-2. B : transmembrane protein 16A (TMEM16A) protein expression normalized to β-actin for quantification of control and DSS-colitis groups. TMEM16A expression (closed bar) is significantly decreased in DSS-colitis mice as compared with control. C and D : summarized data of Western blot quantification of BEST2 and CFTR normalized to β-actin in control and DSS-colitis mice. Neither protein was significantly altered from the control cohort. Bar graphs of summarized data represent means ± SE of 5 different animals from each respective group. * P

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: Pathogenic Roles of Ion Channels and Transporters: Dextran sulfate sodium-induced chronic colitis attenuates Ca2+-activated Cl− secretion in murine colon by downregulating TMEM16A

    doi: 10.1152/ajpcell.00328.2017

    Figure Lengend Snippet: Effect of dextran sulfate sodium (DSS)-colitis on Cl − channel protein expression in murine colon. A : representative images of protein expression of the different characterized Cl − channels from epithelial lysates. BEST2, bestrophin-2. B : transmembrane protein 16A (TMEM16A) protein expression normalized to β-actin for quantification of control and DSS-colitis groups. TMEM16A expression (closed bar) is significantly decreased in DSS-colitis mice as compared with control. C and D : summarized data of Western blot quantification of BEST2 and CFTR normalized to β-actin in control and DSS-colitis mice. Neither protein was significantly altered from the control cohort. Bar graphs of summarized data represent means ± SE of 5 different animals from each respective group. * P

    Article Snippet: PVDF membranes were then incubated in primary antibody: TMEM16A 1:500, CFTR 1:200, bestrophin-2 (BEST2) 1:500, muscarinic 3 (M3 ) receptor 1:200 (Alomone Laboratories), inositol (1,4,5)-trisphosphate (IP3 ) receptor 1:500, β-actin 1:1,000 (Cell Signaling, Danvers, MA) overnight at 4°C.

    Techniques: Expressing, Mouse Assay, Western Blot

    Effect of dextran sulfate sodium (DSS)-colitis on transcript abundance of Cl − channels in murine colon. Transcript abundance was determined for transmembrane protein 16A ( Tmem16a ; A ), bestrophin-2 ( Best2 ; B ), and Cftr ( C ) in control (open bars) or DSS-colitis mice (closed bars). Bar graphs of summarized data represent means ± SE of 5 different animals normalized to an endogenous control, Actb . * P

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: Pathogenic Roles of Ion Channels and Transporters: Dextran sulfate sodium-induced chronic colitis attenuates Ca2+-activated Cl− secretion in murine colon by downregulating TMEM16A

    doi: 10.1152/ajpcell.00328.2017

    Figure Lengend Snippet: Effect of dextran sulfate sodium (DSS)-colitis on transcript abundance of Cl − channels in murine colon. Transcript abundance was determined for transmembrane protein 16A ( Tmem16a ; A ), bestrophin-2 ( Best2 ; B ), and Cftr ( C ) in control (open bars) or DSS-colitis mice (closed bars). Bar graphs of summarized data represent means ± SE of 5 different animals normalized to an endogenous control, Actb . * P

    Article Snippet: PVDF membranes were then incubated in primary antibody: TMEM16A 1:500, CFTR 1:200, bestrophin-2 (BEST2) 1:500, muscarinic 3 (M3 ) receptor 1:200 (Alomone Laboratories), inositol (1,4,5)-trisphosphate (IP3 ) receptor 1:500, β-actin 1:1,000 (Cell Signaling, Danvers, MA) overnight at 4°C.

    Techniques: Mouse Assay

    Bestrophin-1 is expressed in the spinal cord and DRG. RT-PCR ( a , b ) and western blot ( c , d ) analysis of bestrophin-1 (Best-1) at the ipsilateral dorsal portion of the spinal cord (SC) and DRG obtained from naïve (Na), sham (S) and spinal nerve ligated (SNL) rats. Insets show a representative band of the PCR product ( upper panels ) and immunoblot ( lower panels ) obtained with the specific bestrophin-1 and β-actin primers or antibodies, respectively. Data were normalized against β-actin and are expressed as the mean ± SEM of three independent rats. Note that spinal nerve injury did not modify Best-1 mRNA and protein expression.

    Journal: Molecular Pain

    Article Title: Role of anoctamin-1 and bestrophin-1 in spinal nerve ligation-induced neuropathic pain in rats

    doi: 10.1186/s12990-015-0042-1

    Figure Lengend Snippet: Bestrophin-1 is expressed in the spinal cord and DRG. RT-PCR ( a , b ) and western blot ( c , d ) analysis of bestrophin-1 (Best-1) at the ipsilateral dorsal portion of the spinal cord (SC) and DRG obtained from naïve (Na), sham (S) and spinal nerve ligated (SNL) rats. Insets show a representative band of the PCR product ( upper panels ) and immunoblot ( lower panels ) obtained with the specific bestrophin-1 and β-actin primers or antibodies, respectively. Data were normalized against β-actin and are expressed as the mean ± SEM of three independent rats. Note that spinal nerve injury did not modify Best-1 mRNA and protein expression.

    Article Snippet: Membranes were blocked with 5% non-fat milk in phosphate-buffered saline at pH 7.4 containing (in mM) (137 NaCl, 2.7 KCl, 10 Na2 HPO4 and 2 KH2 PO4) with Tween 0.05% and they were incubated at 4°C overnight with rabbit anti-bestrophin-1 (ABC-001, 1:200; Alomone Labs, Jerusalem, Israel) or rabbit anti-anoctamin-1 (ACL-011, 1:100; Alomone Labs, Jerusalem, Israel).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Western Blot, Polymerase Chain Reaction, Expressing

    Intrathecal administration of selective antibody anti-bestrophin-1 or anti-anoctamin-1 reverses established allodynia and reduces protein expression. Time-course of the antiallodynic effect of antibodies against bestrophin-1 (Best-1 ab, 2 μg/6 h × 30 h; a ) or anoctamin-1 (Ano-1 ab, 2 μg/6 h x 30 h; d ) in rats subjected to L5/L6 spinal nerve ligation. Paw withdrawal threshold was assessed every 6 h starting 12 days after spinal nerve ligation. Data are presented as the mean ± SEM for six animals. *Significantly different from the vehicle group (p

    Journal: Molecular Pain

    Article Title: Role of anoctamin-1 and bestrophin-1 in spinal nerve ligation-induced neuropathic pain in rats

    doi: 10.1186/s12990-015-0042-1

    Figure Lengend Snippet: Intrathecal administration of selective antibody anti-bestrophin-1 or anti-anoctamin-1 reverses established allodynia and reduces protein expression. Time-course of the antiallodynic effect of antibodies against bestrophin-1 (Best-1 ab, 2 μg/6 h × 30 h; a ) or anoctamin-1 (Ano-1 ab, 2 μg/6 h x 30 h; d ) in rats subjected to L5/L6 spinal nerve ligation. Paw withdrawal threshold was assessed every 6 h starting 12 days after spinal nerve ligation. Data are presented as the mean ± SEM for six animals. *Significantly different from the vehicle group (p

    Article Snippet: Membranes were blocked with 5% non-fat milk in phosphate-buffered saline at pH 7.4 containing (in mM) (137 NaCl, 2.7 KCl, 10 Na2 HPO4 and 2 KH2 PO4) with Tween 0.05% and they were incubated at 4°C overnight with rabbit anti-bestrophin-1 (ABC-001, 1:200; Alomone Labs, Jerusalem, Israel) or rabbit anti-anoctamin-1 (ACL-011, 1:100; Alomone Labs, Jerusalem, Israel).

    Techniques: Expressing, Ligation

    Bestrophin-1 is expressed in the spinal cord and DRG. RT-PCR ( a , b ) and western blot ( c , d ) analysis of bestrophin-1 (Best-1) at the ipsilateral dorsal portion of the spinal cord (SC) and DRG obtained from naïve (Na), sham (S) and spinal nerve ligated (SNL) rats. Insets show a representative band of the PCR product ( upper panels ) and immunoblot ( lower panels ) obtained with the specific bestrophin-1 and β-actin primers or antibodies, respectively. Data were normalized against β-actin and are expressed as the mean ± SEM of three independent rats. Note that spinal nerve injury did not modify Best-1 mRNA and protein expression.

    Journal: Molecular Pain

    Article Title: Role of anoctamin-1 and bestrophin-1 in spinal nerve ligation-induced neuropathic pain in rats

    doi: 10.1186/s12990-015-0042-1

    Figure Lengend Snippet: Bestrophin-1 is expressed in the spinal cord and DRG. RT-PCR ( a , b ) and western blot ( c , d ) analysis of bestrophin-1 (Best-1) at the ipsilateral dorsal portion of the spinal cord (SC) and DRG obtained from naïve (Na), sham (S) and spinal nerve ligated (SNL) rats. Insets show a representative band of the PCR product ( upper panels ) and immunoblot ( lower panels ) obtained with the specific bestrophin-1 and β-actin primers or antibodies, respectively. Data were normalized against β-actin and are expressed as the mean ± SEM of three independent rats. Note that spinal nerve injury did not modify Best-1 mRNA and protein expression.

    Article Snippet: In addition, antibodies were pre-adsorbed with control bestrophin-1 and anoctamin-1 peptides to validate antibody specificity.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Western Blot, Polymerase Chain Reaction, Expressing

    Intrathecal administration of selective antibody anti-bestrophin-1 or anti-anoctamin-1 reverses established allodynia and reduces protein expression. Time-course of the antiallodynic effect of antibodies against bestrophin-1 (Best-1 ab, 2 μg/6 h × 30 h; a ) or anoctamin-1 (Ano-1 ab, 2 μg/6 h x 30 h; d ) in rats subjected to L5/L6 spinal nerve ligation. Paw withdrawal threshold was assessed every 6 h starting 12 days after spinal nerve ligation. Data are presented as the mean ± SEM for six animals. *Significantly different from the vehicle group (p

    Journal: Molecular Pain

    Article Title: Role of anoctamin-1 and bestrophin-1 in spinal nerve ligation-induced neuropathic pain in rats

    doi: 10.1186/s12990-015-0042-1

    Figure Lengend Snippet: Intrathecal administration of selective antibody anti-bestrophin-1 or anti-anoctamin-1 reverses established allodynia and reduces protein expression. Time-course of the antiallodynic effect of antibodies against bestrophin-1 (Best-1 ab, 2 μg/6 h × 30 h; a ) or anoctamin-1 (Ano-1 ab, 2 μg/6 h x 30 h; d ) in rats subjected to L5/L6 spinal nerve ligation. Paw withdrawal threshold was assessed every 6 h starting 12 days after spinal nerve ligation. Data are presented as the mean ± SEM for six animals. *Significantly different from the vehicle group (p

    Article Snippet: In addition, antibodies were pre-adsorbed with control bestrophin-1 and anoctamin-1 peptides to validate antibody specificity.

    Techniques: Expressing, Ligation