Structured Review

GE Healthcare benzamidine sepharose 6b
SDS-PAGE analysis of purified protease. Concentrated culture supernatant was applied to a <t>benzamidine-Sepharose</t> 6B column (2 ml) in 50 mM Tris-HCl (pH 8.0)-0.5 M NaCl. The retained enzyme was eluted by applying 0.1 M glycine-HCl (pH 3.0). Fractions of 1 ml were collected and monitored for enzyme activity. Enzyme activity was determined by using azocoll as a substrate. Fractions showing proteolytic activity were pooled. Protein concentration was determined by the Bradford method, and this fraction was subjected to SDS-PAGE analysis under reducing conditions.
Benzamidine Sepharose 6b, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 20 article reviews
Price from $9.99 to $1999.99
benzamidine sepharose 6b - by Bioz Stars, 2020-04
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1) Product Images from "Proteome and Transcriptome Analysis of the Virulence Genes Regulated by the VirR/VirS System in Clostridium perfringens"

Article Title: Proteome and Transcriptome Analysis of the Virulence Genes Regulated by the VirR/VirS System in Clostridium perfringens

Journal: Journal of Bacteriology

doi: 10.1128/JB.184.10.2587-2594.2002

SDS-PAGE analysis of purified protease. Concentrated culture supernatant was applied to a benzamidine-Sepharose 6B column (2 ml) in 50 mM Tris-HCl (pH 8.0)-0.5 M NaCl. The retained enzyme was eluted by applying 0.1 M glycine-HCl (pH 3.0). Fractions of 1 ml were collected and monitored for enzyme activity. Enzyme activity was determined by using azocoll as a substrate. Fractions showing proteolytic activity were pooled. Protein concentration was determined by the Bradford method, and this fraction was subjected to SDS-PAGE analysis under reducing conditions.
Figure Legend Snippet: SDS-PAGE analysis of purified protease. Concentrated culture supernatant was applied to a benzamidine-Sepharose 6B column (2 ml) in 50 mM Tris-HCl (pH 8.0)-0.5 M NaCl. The retained enzyme was eluted by applying 0.1 M glycine-HCl (pH 3.0). Fractions of 1 ml were collected and monitored for enzyme activity. Enzyme activity was determined by using azocoll as a substrate. Fractions showing proteolytic activity were pooled. Protein concentration was determined by the Bradford method, and this fraction was subjected to SDS-PAGE analysis under reducing conditions.

Techniques Used: SDS Page, Purification, Activity Assay, Protein Concentration

Related Articles

Clone Assay:

Article Title: Calcium-Dependent Assembly of Centrin-G-Protein Complex in Photoreceptor Cells
Article Snippet: Mouse centrin 1 cDNA was kindly provided by Jeff L. Salisbury, Mayo Clinic Foundation, Rochester, Minn., and was cloned into the pGEX-4T3 expression vector (Amersham Biosciences) using Bam HI and Eco RI restriction sites. .. After cleavage of the fusion protein with thrombin on the column, centrin was eluted in 20 mM BTP (pH 7.5)-130 mM NaCl-1 mM MgCl2 and passed over a benzamidine Sepharose 6B (Amersham Biosciences) column to remove thrombin.

Article Title: The crystal structure of the ttCsaA protein: an export-related chaperone from Thermus thermophilus
Article Snippet: Paragraph title: Cloning, expression and purification ... The resulting fraction was again loaded onto the Ni2+ -NTA column to remove the cleaved His-tag, and was then loaded onto a benzamidine Sepharose 6B (Amersham Pharmacia Biotech.) column to remove the thrombin.

Centrifugation:

Article Title: A Sensitive Fluorescence-Based Assay for the Detection of ExoU-Mediated PLA2 Activity
Article Snippet: The total bacterial lysate was subjected to centrifugation at 16,200 × g for 10 min to separate unbroken cells and membrane debris from the smaller cellular components. .. The digested material was passed through Sephacryl S100HR (Sigma) and benzamidine Sepharose 6B (GE Healthcare) resins to remove the histidine tag and thrombin, respectively.

Article Title: The crystal structure of the ttCsaA protein: an export-related chaperone from Thermus thermophilus
Article Snippet: The cleared cell extract was obtained by centrifugation at 15 000 r.p.m. for 30 min (Hitachi 46) and was loaded onto a Ni2+ -nitrilotriacetic agarose (NTA) column (Invitrogen) (3 ml bed volume). .. The resulting fraction was again loaded onto the Ni2+ -NTA column to remove the cleaved His-tag, and was then loaded onto a benzamidine Sepharose 6B (Amersham Pharmacia Biotech.) column to remove the thrombin.

Filtration:

Article Title: Nucleoprotein complex intermediates in HIV-1 integration
Article Snippet: Remove thrombin from the preparation by adsorption to a column of benzamidine-Sepharose 6B (GE Healthcare) equilibrated with Equilibration buffer B . .. Concentrate the integrase with an Amicon Ultra 15 centrifugal filter (Millipore) and load onto a Superdex 75 column (GE Healthcare) equilibrated with Gel filtration buffer .

Adsorption:

Article Title: Nucleoprotein complex intermediates in HIV-1 integration
Article Snippet: .. Remove thrombin from the preparation by adsorption to a column of benzamidine-Sepharose 6B (GE Healthcare) equilibrated with Equilibration buffer B . .. Concentrate the integrase with an Amicon Ultra 15 centrifugal filter (Millipore) and load onto a Superdex 75 column (GE Healthcare) equilibrated with Gel filtration buffer .

Article Title: Assembly of prototype foamy virus strand transfer complexes on product DNA bypassing catalysis of integration
Article Snippet: .. Thrombin was removed by adsorption to benzamidine-Sepharose 6B (GE Healthcare). .. The protein was dialyzed against 20 m M HEPES, pH 7.5, 0.2 M NaCl, 2 m M dithiothreitol (DTT), 1 m M EDTA, 10% glycerol, loaded onto a Mono S 10/10 column (GE Healthcare) and eluted with a linear gradient of 0.2–0.6 M NaCl in 20 m M HEPES, pH 7.5, 2 m M DTT, 1 m M EDTA, 10% glycerol.

Incubation:

Article Title: Nucleoprotein complex intermediates in HIV-1 integration
Article Snippet: Continue the incubation if necessary until the His-tag is completely removed (His-tag removal can also be accomplished by adding thrombin to the integrase during the dialysis step at 4 °C overnight. .. Remove thrombin from the preparation by adsorption to a column of benzamidine-Sepharose 6B (GE Healthcare) equilibrated with Equilibration buffer B .

Activity Assay:

Article Title: Proteome and Transcriptome Analysis of the Virulence Genes Regulated by the VirR/VirS System in Clostridium perfringens
Article Snippet: Concentrated supernatant solution was applied to a benzamidine-Sepharose 6B (Amersham Pharmacia Biotech United Kingdom, Ltd., Buckinghamshire, England) affinity column (bed volume, 2 ml) equilibrated with 50 mM Tris-HCl (pH 8.0) containing 0.5 M NaCl. .. Fractions of 1 ml were collected in a tube that contained 0.3 ml of 0.1 M Tris-HCl (pH 9.0), and the protease activity was assayed.

Expressing:

Article Title: Assembly of prototype foamy virus strand transfer complexes on product DNA bypassing catalysis of integration
Article Snippet: Paragraph title: PFV IN expression and purification ... Thrombin was removed by adsorption to benzamidine-Sepharose 6B (GE Healthcare).

Article Title: A Sensitive Fluorescence-Based Assay for the Detection of ExoU-Mediated PLA2 Activity
Article Snippet: Paragraph title: 2.3. Protein expression, SDS PAGE, and immunoblot analysis ... The digested material was passed through Sephacryl S100HR (Sigma) and benzamidine Sepharose 6B (GE Healthcare) resins to remove the histidine tag and thrombin, respectively.

Article Title: Calcium-Dependent Assembly of Centrin-G-Protein Complex in Photoreceptor Cells
Article Snippet: Paragraph title: Centrin 1 expression. ... After cleavage of the fusion protein with thrombin on the column, centrin was eluted in 20 mM BTP (pH 7.5)-130 mM NaCl-1 mM MgCl2 and passed over a benzamidine Sepharose 6B (Amersham Biosciences) column to remove thrombin.

Article Title: The crystal structure of the ttCsaA protein: an export-related chaperone from Thermus thermophilus
Article Snippet: Paragraph title: Cloning, expression and purification ... The resulting fraction was again loaded onto the Ni2+ -NTA column to remove the cleaved His-tag, and was then loaded onto a benzamidine Sepharose 6B (Amersham Pharmacia Biotech.) column to remove the thrombin.

Article Title: mNUDC is required for plus-end-directed transport of cytoplasmic dynein and dynactins by kinesin-1
Article Snippet: Paragraph title: Vectors used for expression in DRG and recombinant proteins ... To remove GST tag, we treated recombinant protein with thrombin (Merck), followed by absorption of thrombin by benzamidine Sepharose 6B (GE Healthcare Biosciences).

Spin Trapping:

Article Title: Aldehyde Oxidase Functions as a Superoxide Generating NADH Oxidase: An Important Redox Regulated Pathway of Cellular Oxygen Radical Formation
Article Snippet: Benzamidine Sepharose 6B, Sephadex G-25, and molecular weight marker for native gel were obtained from GE-Healthcare Bio-Sciences. .. Spin trapping agent, 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline- N -oxide (DEPMPO) was purchased from Alexis (Carlsbad, CA).

Transformation Assay:

Article Title: The crystal structure of the ttCsaA protein: an export-related chaperone from Thermus thermophilus
Article Snippet: The resulting plasmid, pET15b- ttcsaA , was transformed into E.coli strain JM109(DE3). .. The resulting fraction was again loaded onto the Ni2+ -NTA column to remove the cleaved His-tag, and was then loaded onto a benzamidine Sepharose 6B (Amersham Pharmacia Biotech.) column to remove the thrombin.

High Performance Liquid Chromatography:

Article Title: Simple Preparation of Pacific Cod Trypsin for Enzymatic Peptide Synthesis
Article Snippet: N -p -Tosyl-L-arginine methyl ester hydrochloride (TAME), N -benzoyl-L-arginine p -nitroanilide hydrochlodide (BAPA), p -nitrophenyl-p ′-guanidinobenzoate hydrochloride (NPGB), tris(hydroxymethyl)aminomethane (Tris), and 3,5-dimethoxy-4-hydroxycinnamic acid (sinapinic acid) ware obtained from Sigma-Aldrich (Tokyo, Japan). p -Nitrophenol, HPLC grade dimethyl sulfoxide (DMSO), acetone, and acetonitril were purchased from Kanto Chemical Co. Inc. (Tokyo, Japan). .. Benzamidine Sepharose 6B and Superdex 75 were purchased from GE Healthcare (Buckinghamshire, England).

Generated:

Article Title: mNUDC is required for plus-end-directed transport of cytoplasmic dynein and dynactins by kinesin-1
Article Snippet: Recombinant protein for kinesin light chain1 or mNUDC was generated using pGEX-4T expression vector (GE Healthcare Biosciences). .. To remove GST tag, we treated recombinant protein with thrombin (Merck), followed by absorption of thrombin by benzamidine Sepharose 6B (GE Healthcare Biosciences).

Sonication:

Article Title: Overproduction, purification and preliminary X-ray diffraction analysis of a sulfotransferase from Mycobacterium tuberculosis H37Rv
Article Snippet: .. Finally, the resin with bound fusion protein was suspended in 5 ml sonication buffer containing 20 units thrombin (Sigma) and digested for 12 h at 283 K. After digestion, the supernatant was passed through a Benzamidine Sepharose 6B (Amersham Biosciences) column to remove thrombin and concentrated to 0.6 mg ml−1 by ultrafiltration (Amicon Ultra-4, 10 kDa cutoff, Millipore). .. The concentrated protein was then run through a Superose gel-filtration column (Amersham Biosciences) using the sonication buffer.

Recombinant:

Article Title: mNUDC is required for plus-end-directed transport of cytoplasmic dynein and dynactins by kinesin-1
Article Snippet: .. To remove GST tag, we treated recombinant protein with thrombin (Merck), followed by absorption of thrombin by benzamidine Sepharose 6B (GE Healthcare Biosciences). .. The DRGs from post-natal mice were dissociated using a previously described method ( ).

Molecular Weight:

Article Title: Binding and Functional Properties of Five Extrinsic Proteins in Oxygen-evolving Photosystem II from a Marine Centric Diatom, Chaetoceros gracilis *
Article Snippet: .. The supernatants were dialyzed using Spectra/Por dialysis membrane (molecular weight cutoff, 8000) (Spectrum Laboratories) against buffer C after addition of 1 m m PMSF, 1 m m EDTA, and 10 μl of benzamidine-Sepharose 6B (GE Healthcare) to prevent cleavages of the extrinsic proteins by endogenous proteases. ..

Article Title: Aldehyde Oxidase Functions as a Superoxide Generating NADH Oxidase: An Important Redox Regulated Pathway of Cellular Oxygen Radical Formation
Article Snippet: .. Benzamidine Sepharose 6B, Sephadex G-25, and molecular weight marker for native gel were obtained from GE-Healthcare Bio-Sciences. .. BioSepra Hydroxyapatite Ultrogel was purchased from Paal Life Sciences, PA. Diphenyleneiodonium chloride (DPI) was purchased from Cayman Chemical and amidone from Xanodyne Pharmaceuticals Inc., Newport, KY. Diethylenetriaminepentaacetic acid (DTPA) was purchased from Aldrich.

Nucleic Acid Electrophoresis:

Article Title: Nucleoprotein complex intermediates in HIV-1 integration
Article Snippet: Incubate at 25 °C for 1 h. Place on ice and check the completeness of thrombin digestion by SDS–polyacrylamide gel electrophoresis (PAGE). .. Remove thrombin from the preparation by adsorption to a column of benzamidine-Sepharose 6B (GE Healthcare) equilibrated with Equilibration buffer B .

Avidin-Biotin Assay:

Article Title: Biochemical characterization of hyperactive ?2-chimaerin mutants revealed an enhanced exposure of C1 and Rac-GAP domains †
Article Snippet: L-α-phosphatidylcholine, 1,2-diacyl-sn-glycero-3-phospho-L-serine, and avidin high capacity agarose were obtained from Sigma. .. Thrombin, Glutathione Sepharose 4B, and Benzamidine Sepharose 6B were purchased from Amersham Biosciences (Pittsburgh, PA).

Purification:

Article Title: Nucleoprotein complex intermediates in HIV-1 integration
Article Snippet: Paragraph title: 2.2.2. Purification of HIV-1 integrase ... Remove thrombin from the preparation by adsorption to a column of benzamidine-Sepharose 6B (GE Healthcare) equilibrated with Equilibration buffer B .

Article Title: Assembly of prototype foamy virus strand transfer complexes on product DNA bypassing catalysis of integration
Article Snippet: Paragraph title: PFV IN expression and purification ... Thrombin was removed by adsorption to benzamidine-Sepharose 6B (GE Healthcare).

Article Title: A Sensitive Fluorescence-Based Assay for the Detection of ExoU-Mediated PLA2 Activity
Article Snippet: The digested material was passed through Sephacryl S100HR (Sigma) and benzamidine Sepharose 6B (GE Healthcare) resins to remove the histidine tag and thrombin, respectively. .. This protein was purified by Talon Co+2 affinity chromatography and dialyzed against 50 mmol/l NaCl and 50 mmol/l MOPS pH 6.3.

Article Title: Binding and Functional Properties of Five Extrinsic Proteins in Oxygen-evolving Photosystem II from a Marine Centric Diatom, Chaetoceros gracilis *
Article Snippet: Paragraph title: Purification Procedures of Five Extrinsic Proteins ... The supernatants were dialyzed using Spectra/Por dialysis membrane (molecular weight cutoff, 8000) (Spectrum Laboratories) against buffer C after addition of 1 m m PMSF, 1 m m EDTA, and 10 μl of benzamidine-Sepharose 6B (GE Healthcare) to prevent cleavages of the extrinsic proteins by endogenous proteases.

Article Title: Overproduction, purification and preliminary X-ray diffraction analysis of a sulfotransferase from Mycobacterium tuberculosis H37Rv
Article Snippet: Paragraph title: 2.2. Purification ... Finally, the resin with bound fusion protein was suspended in 5 ml sonication buffer containing 20 units thrombin (Sigma) and digested for 12 h at 283 K. After digestion, the supernatant was passed through a Benzamidine Sepharose 6B (Amersham Biosciences) column to remove thrombin and concentrated to 0.6 mg ml−1 by ultrafiltration (Amicon Ultra-4, 10 kDa cutoff, Millipore).

Article Title: Proteome and Transcriptome Analysis of the Virulence Genes Regulated by the VirR/VirS System in Clostridium perfringens
Article Snippet: Paragraph title: Purification of protease. ... Concentrated supernatant solution was applied to a benzamidine-Sepharose 6B (Amersham Pharmacia Biotech United Kingdom, Ltd., Buckinghamshire, England) affinity column (bed volume, 2 ml) equilibrated with 50 mM Tris-HCl (pH 8.0) containing 0.5 M NaCl.

Article Title: Calcium-Dependent Assembly of Centrin-G-Protein Complex in Photoreceptor Cells
Article Snippet: Expression and purification of the GST fusion protein was performed according to the manufacturer's instructions. .. After cleavage of the fusion protein with thrombin on the column, centrin was eluted in 20 mM BTP (pH 7.5)-130 mM NaCl-1 mM MgCl2 and passed over a benzamidine Sepharose 6B (Amersham Biosciences) column to remove thrombin.

Article Title: The crystal structure of the ttCsaA protein: an export-related chaperone from Thermus thermophilus
Article Snippet: Paragraph title: Cloning, expression and purification ... The resulting fraction was again loaded onto the Ni2+ -NTA column to remove the cleaved His-tag, and was then loaded onto a benzamidine Sepharose 6B (Amersham Pharmacia Biotech.) column to remove the thrombin.

Protein Purification:

Article Title: mNUDC is required for plus-end-directed transport of cytoplasmic dynein and dynactins by kinesin-1
Article Snippet: Protein purification was performed using glutathione Sepharose 4B (GE Healthcare Biosciences) according to the manufacturer's recommendation. .. To remove GST tag, we treated recombinant protein with thrombin (Merck), followed by absorption of thrombin by benzamidine Sepharose 6B (GE Healthcare Biosciences).

Affinity Column:

Article Title: Proteome and Transcriptome Analysis of the Virulence Genes Regulated by the VirR/VirS System in Clostridium perfringens
Article Snippet: .. Concentrated supernatant solution was applied to a benzamidine-Sepharose 6B (Amersham Pharmacia Biotech United Kingdom, Ltd., Buckinghamshire, England) affinity column (bed volume, 2 ml) equilibrated with 50 mM Tris-HCl (pH 8.0) containing 0.5 M NaCl. ..

Polyacrylamide Gel Electrophoresis:

Article Title: Nucleoprotein complex intermediates in HIV-1 integration
Article Snippet: Incubate at 25 °C for 1 h. Place on ice and check the completeness of thrombin digestion by SDS–polyacrylamide gel electrophoresis (PAGE). .. Remove thrombin from the preparation by adsorption to a column of benzamidine-Sepharose 6B (GE Healthcare) equilibrated with Equilibration buffer B .

Lysis:

Article Title: Assembly of prototype foamy virus strand transfer complexes on product DNA bypassing catalysis of integration
Article Snippet: The cell paste was suspended in lysis buffer (20 m M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), pH 7.5, 20 m M imidazole, 0.5 M NaCl, 2 m M 2-mercaptoehanol, 0.4 mg/mL lysozyme). .. Thrombin was removed by adsorption to benzamidine-Sepharose 6B (GE Healthcare).

SDS Page:

Article Title: A Sensitive Fluorescence-Based Assay for the Detection of ExoU-Mediated PLA2 Activity
Article Snippet: Paragraph title: 2.3. Protein expression, SDS PAGE, and immunoblot analysis ... The digested material was passed through Sephacryl S100HR (Sigma) and benzamidine Sepharose 6B (GE Healthcare) resins to remove the histidine tag and thrombin, respectively.

Article Title: Overproduction, purification and preliminary X-ray diffraction analysis of a sulfotransferase from Mycobacterium tuberculosis H37Rv
Article Snippet: Finally, the resin with bound fusion protein was suspended in 5 ml sonication buffer containing 20 units thrombin (Sigma) and digested for 12 h at 283 K. After digestion, the supernatant was passed through a Benzamidine Sepharose 6B (Amersham Biosciences) column to remove thrombin and concentrated to 0.6 mg ml−1 by ultrafiltration (Amicon Ultra-4, 10 kDa cutoff, Millipore). .. The purity of the prepared sample was judged using 10% SDS–PAGE stained with Coomassie Brilliant Blue.

Plasmid Preparation:

Article Title: Calcium-Dependent Assembly of Centrin-G-Protein Complex in Photoreceptor Cells
Article Snippet: Mouse centrin 1 cDNA was kindly provided by Jeff L. Salisbury, Mayo Clinic Foundation, Rochester, Minn., and was cloned into the pGEX-4T3 expression vector (Amersham Biosciences) using Bam HI and Eco RI restriction sites. .. After cleavage of the fusion protein with thrombin on the column, centrin was eluted in 20 mM BTP (pH 7.5)-130 mM NaCl-1 mM MgCl2 and passed over a benzamidine Sepharose 6B (Amersham Biosciences) column to remove thrombin.

Article Title: The crystal structure of the ttCsaA protein: an export-related chaperone from Thermus thermophilus
Article Snippet: The resulting plasmid, pET15b- ttcsaA , was transformed into E.coli strain JM109(DE3). .. The resulting fraction was again loaded onto the Ni2+ -NTA column to remove the cleaved His-tag, and was then loaded onto a benzamidine Sepharose 6B (Amersham Pharmacia Biotech.) column to remove the thrombin.

Article Title: mNUDC is required for plus-end-directed transport of cytoplasmic dynein and dynactins by kinesin-1
Article Snippet: Recombinant protein for kinesin light chain1 or mNUDC was generated using pGEX-4T expression vector (GE Healthcare Biosciences). .. To remove GST tag, we treated recombinant protein with thrombin (Merck), followed by absorption of thrombin by benzamidine Sepharose 6B (GE Healthcare Biosciences).

Homogenization:

Article Title: Simple Preparation of Pacific Cod Trypsin for Enzymatic Peptide Synthesis
Article Snippet: The pyloric caeca of Pacific cod were separated from other intestinal organs and were stored at −84°C prior to homogenization. .. Benzamidine Sepharose 6B and Superdex 75 were purchased from GE Healthcare (Buckinghamshire, England).

Concentration Assay:

Article Title: Assembly of prototype foamy virus strand transfer complexes on product DNA bypassing catalysis of integration
Article Snippet: The histidine tag was removed using thrombin (Sigma) at a concentration of 6 NIH U/mg of protein. .. Thrombin was removed by adsorption to benzamidine-Sepharose 6B (GE Healthcare).

Marker:

Article Title: Aldehyde Oxidase Functions as a Superoxide Generating NADH Oxidase: An Important Redox Regulated Pathway of Cellular Oxygen Radical Formation
Article Snippet: .. Benzamidine Sepharose 6B, Sephadex G-25, and molecular weight marker for native gel were obtained from GE-Healthcare Bio-Sciences. .. BioSepra Hydroxyapatite Ultrogel was purchased from Paal Life Sciences, PA. Diphenyleneiodonium chloride (DPI) was purchased from Cayman Chemical and amidone from Xanodyne Pharmaceuticals Inc., Newport, KY. Diethylenetriaminepentaacetic acid (DTPA) was purchased from Aldrich.

Staining:

Article Title: Overproduction, purification and preliminary X-ray diffraction analysis of a sulfotransferase from Mycobacterium tuberculosis H37Rv
Article Snippet: Finally, the resin with bound fusion protein was suspended in 5 ml sonication buffer containing 20 units thrombin (Sigma) and digested for 12 h at 283 K. After digestion, the supernatant was passed through a Benzamidine Sepharose 6B (Amersham Biosciences) column to remove thrombin and concentrated to 0.6 mg ml−1 by ultrafiltration (Amicon Ultra-4, 10 kDa cutoff, Millipore). .. The purity of the prepared sample was judged using 10% SDS–PAGE stained with Coomassie Brilliant Blue.

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    GE Healthcare benzamidine
    Inhibition by HAI-1–58K of MAT molecules with and without purification by <t>benzamidine</t> chromatography. A , SDS-PAGE and silver staining showing the removal of fragment Tyr 81 –Lys 379 . MAT before treatment with immobilized benzamidine ( lane
    Benzamidine, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/benzamidine/product/GE Healthcare
    Average 94 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    benzamidine - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    93
    GE Healthcare affinity resin benzamidine sepharose 6b
    Affinity liquid chromatography of Crotalus durissus terrificus crude venom with <t>Benzamidine-Sepharose</t> 6B resin (column AxiChrom 100/300® (100 mm × 300 mm × 350 mL). The column was equilibrated with 0.05 M Tris-HCl pH 7.4 (buffer 1). Sample: 15 g of crude venom was suspended in buffer 1. The sample elution was carried out with 525 mL of buffer 1 followed by 525 mL of 0.05 M Tris-HCl/0.5 M NaCl pH 7.4 (buffer 2) and 1,050 mL of 0.02 M glycine pH 3.2 (buffer 3), at flow rate 10 mL/min, and collected 25 mL/tube. Absorbance 280 nm
    Affinity Resin Benzamidine Sepharose 6b, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/affinity resin benzamidine sepharose 6b/product/GE Healthcare
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    affinity resin benzamidine sepharose 6b - by Bioz Stars, 2020-04
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    92
    GE Healthcare benzamidine sepharose 6b
    Affinity liquid chromatography of Crotalus durissus terrificus crude venom with <t>Benzamidine-Sepharose</t> 6B resin (column AxiChrom 100/300® (100 mm × 300 mm × 350 mL). The column was equilibrated with 0.05 M Tris-HCl pH 7.4 (buffer 1). Sample: 15 g of crude venom was suspended in buffer 1. The sample elution was carried out with 525 mL of buffer 1 followed by 525 mL of 0.05 M Tris-HCl/0.5 M NaCl pH 7.4 (buffer 2) and 1,050 mL of 0.02 M glycine pH 3.2 (buffer 3), at flow rate 10 mL/min, and collected 25 mL/tube. Absorbance 280 nm
    Benzamidine Sepharose 6b, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/benzamidine sepharose 6b/product/GE Healthcare
    Average 92 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    benzamidine sepharose 6b - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    93
    GE Healthcare benzamidine sepharose 6b column
    Affinity liquid chromatography of Crotalus durissus terrificus crude venom with <t>Benzamidine-Sepharose</t> 6B resin (column AxiChrom 100/300® (100 mm × 300 mm × 350 mL). The column was equilibrated with 0.05 M Tris-HCl pH 7.4 (buffer 1). Sample: 15 g of crude venom was suspended in buffer 1. The sample elution was carried out with 525 mL of buffer 1 followed by 525 mL of 0.05 M Tris-HCl/0.5 M NaCl pH 7.4 (buffer 2) and 1,050 mL of 0.02 M glycine pH 3.2 (buffer 3), at flow rate 10 mL/min, and collected 25 mL/tube. Absorbance 280 nm
    Benzamidine Sepharose 6b Column, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/benzamidine sepharose 6b column/product/GE Healthcare
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    benzamidine sepharose 6b column - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    Image Search Results


    Inhibition by HAI-1–58K of MAT molecules with and without purification by benzamidine chromatography. A , SDS-PAGE and silver staining showing the removal of fragment Tyr 81 –Lys 379 . MAT before treatment with immobilized benzamidine ( lane

    Journal: The Journal of Biological Chemistry

    Article Title: Identification of the Matriptase Second CUB Domain as the Secondary Site for Interaction with Hepatocyte Growth Factor Activator Inhibitor Type-1 *

    doi: 10.1074/jbc.M110.115816

    Figure Lengend Snippet: Inhibition by HAI-1–58K of MAT molecules with and without purification by benzamidine chromatography. A , SDS-PAGE and silver staining showing the removal of fragment Tyr 81 –Lys 379 . MAT before treatment with immobilized benzamidine ( lane

    Article Snippet: A 0.5-ml sample of HEPES buffer containing r-EK-treated pro-MAT was incubated for 30 min with 50 μl of a slurry of immobilized benzamidine (benzamidine-Sepharose 6B; GE Healthcare) at 22 °C with rocking.

    Techniques: Inhibition, Purification, Chromatography, SDS Page, Silver Staining

    Affinity liquid chromatography of Crotalus durissus terrificus crude venom with Benzamidine-Sepharose 6B resin (column AxiChrom 100/300® (100 mm × 300 mm × 350 mL). The column was equilibrated with 0.05 M Tris-HCl pH 7.4 (buffer 1). Sample: 15 g of crude venom was suspended in buffer 1. The sample elution was carried out with 525 mL of buffer 1 followed by 525 mL of 0.05 M Tris-HCl/0.5 M NaCl pH 7.4 (buffer 2) and 1,050 mL of 0.02 M glycine pH 3.2 (buffer 3), at flow rate 10 mL/min, and collected 25 mL/tube. Absorbance 280 nm

    Journal: The Journal of Venomous Animals and Toxins Including Tropical Diseases

    Article Title: Heterologous fibrin sealant derived from snake venom: from bench to bedside – an overview

    doi: 10.1186/s40409-017-0109-8

    Figure Lengend Snippet: Affinity liquid chromatography of Crotalus durissus terrificus crude venom with Benzamidine-Sepharose 6B resin (column AxiChrom 100/300® (100 mm × 300 mm × 350 mL). The column was equilibrated with 0.05 M Tris-HCl pH 7.4 (buffer 1). Sample: 15 g of crude venom was suspended in buffer 1. The sample elution was carried out with 525 mL of buffer 1 followed by 525 mL of 0.05 M Tris-HCl/0.5 M NaCl pH 7.4 (buffer 2) and 1,050 mL of 0.02 M glycine pH 3.2 (buffer 3), at flow rate 10 mL/min, and collected 25 mL/tube. Absorbance 280 nm

    Article Snippet: This was applied in an AxiChrom 100/300® chromatographic column (100 mm x 300 mm x 350 mL) (GE HealthCare Life Science, Sweden) encased with affinity resin Benzamidine Sepharose 6B® (GE HealthCare Life Science, Sweden) previously equilibrated with 525 mL of 0.05 M Tris-HCl pH 7.4 buffer (buffer 1).

    Techniques: Liquid Chromatography, Flow Cytometry