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Shown here are the two detection channels for the fluorescence signal generated by two-photon absorption. The <t>Non</t> <t>Descanned</t> channel, positioned closest to the objective, is the most sensitive. The <t>GaAsP</t> detectors are new and provide a reference signal optimized for learning. The detection path through the scan head is generally less efficient, even if the pinholes are completely open to collect the scattered emission photons. Multi-Alkali detectors are functional but obsolete, over 15 years old, and will be used to detect ground truth. The signals detected on both types of detectors are almost spatially aligned.
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Shown here are the two detection channels for the fluorescence signal generated by two-photon absorption. The Non Descanned channel, positioned closest to the objective, is the most sensitive. The GaAsP detectors are new and provide a reference signal optimized for learning. The detection path through the scan head is generally less efficient, even if the pinholes are completely open to collect the scattered emission photons. Multi-Alkali detectors are functional but obsolete, over 15 years old, and will be used to detect ground truth. The signals detected on both types of detectors are almost spatially aligned.

Journal: bioRxiv

Article Title: µPIX : Leveraging Generative AI for Enhanced, Personalized and Sustainable Microscopy

doi: 10.1101/2024.10.25.620201

Figure Lengend Snippet: Shown here are the two detection channels for the fluorescence signal generated by two-photon absorption. The Non Descanned channel, positioned closest to the objective, is the most sensitive. The GaAsP detectors are new and provide a reference signal optimized for learning. The detection path through the scan head is generally less efficient, even if the pinholes are completely open to collect the scattered emission photons. Multi-Alkali detectors are functional but obsolete, over 15 years old, and will be used to detect ground truth. The signals detected on both types of detectors are almost spatially aligned.

Article Snippet: First, the more efficient non-descanned (NDD) beam path using two specially integrated Hamamatsu GaAsP detectors (new integration work in 2021 by ALPhANOV company).

Techniques: Fluorescence, Generated, Functional Assay