bcl xl shrna transfected cells  (Jackson Laboratory)

Journal: World Journal of Gastroenterology : WJG

Article Title: Significance of Bcl-xL in human colon carcinoma

doi: 10.3748/wjg.14.3069

Figure Lengend Snippet: Table 1

Article Snippet: Cell culture and Bcl-xL siRNA transfection Human colon cancer cell line HT29 (Institute of Cell Biology, Shanghai, China) was cultured in RPMI 1640 (Invitrogen, Inc.) supplemented with 10% fetal bovine serum (FBS) in an atmosphere containing 50 mL/L CO 2 at 37°C. siRNA was transfected with a commercial reagent, oligofectamine (Invitrogen, USA) in 6-well plates following its manufacturer’s instructions.

Techniques: Expressing