bcci (New England Biolabs)
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New England Biolabs manufactures this product 
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Name:
BccI
Description:
BccI 5 000 units
Catalog Number:
R0704L
Price:
282
Category:
Restriction Enzymes
Size:
5 000 units
|
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Structured Review
New England Biolabs
bcci
BccI 5 000 units
https://www.bioz.com/result/bcci/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
BccI 5 000 units
https://www.bioz.com/result/bcci/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
bcci - by Bioz Stars,
2021-04
94/100 stars
Images
1) Product Images from "Kinesin Family Member 6 (kif6) Is Necessary for Spine Development in Zebrafish"
Article Title: Kinesin Family Member 6 (kif6) Is Necessary for Spine Development in Zebrafish
Journal: Developmental dynamics : an official publication of the American Association of Anatomists
doi: 10.1002/dvdy.24208
Figure Legend Snippet: Design and efficiency of kif6 TALENs. A: Genomic sequence showing left and right TALENs that were designed in the second exon of kif6 near the gw326 mutant allele. The TALEN pair flank a BccI restriction site. B: BccI restriction digest of PCR amplified
Techniques Used: TALENs, Sequencing, Mutagenesis, Polymerase Chain Reaction, Amplification
2) Product Images from "Quantitative and rapid Plasmodium falciparum malaria diagnosis and artemisinin-resistance detection using a CMOS Lab-on-Chip platform"
Article Title: Quantitative and rapid Plasmodium falciparum malaria diagnosis and artemisinin-resistance detection using a CMOS Lab-on-Chip platform
Journal: bioRxiv
doi: 10.1101/638221
![Sequence alignment, primer design and analytical specificity for the detection of P. falciparum . (A) Illustration of the gene kelch 13 showing in black the region selected for primer design. (B) Alignment of all human-infective Plasmodium species with P. falciparum as reference sequence. Mismatches in the alignment are displayed as AGTC whereas matched nucleotides are represented as dots. Primers are illustrated on top of the alignment. (C) Primer location in LAMP amplicon (5’ to 3’ direction). Sequences of the primers can be found in table S1. (D) Results showing specific amplification of P. falciparum DNA samples from clinical isolates as well as gel electrophoresis of the amplified products to confirm specificity. Digested amplification products are shown in fig. S2. The restriction enzyme used for this experiment is BccI (# R0704S, New England BioLabs). The cutting point is illustrated with an arrow and the binding region is orange shadowed in (B). M denotes 100 bp DNA ladder (#10488058, Invitrogen). Samples are described in (B). (E) Pan-primer set [ 21 ] used as reference for the detection of all Plasmodium species. TTP values are displayed in minutes and labelled.](https://www.biorxiv.org/content/biorxiv/early/2019/05/16/638221/F1.large.jpg "... The restriction enzyme used for this experiment is BccI (# R0704S, New England BioLabs). The cutting point ...")
Figure Legend Snippet: Sequence alignment, primer design and analytical specificity for the detection of P. falciparum . (A) Illustration of the gene kelch 13 showing in black the region selected for primer design. (B) Alignment of all human-infective Plasmodium species with P. falciparum as reference sequence. Mismatches in the alignment are displayed as AGTC whereas matched nucleotides are represented as dots. Primers are illustrated on top of the alignment. (C) Primer location in LAMP amplicon (5’ to 3’ direction). Sequences of the primers can be found in table S1. (D) Results showing specific amplification of P. falciparum DNA samples from clinical isolates as well as gel electrophoresis of the amplified products to confirm specificity. Digested amplification products are shown in fig. S2. The restriction enzyme used for this experiment is BccI (# R0704S, New England BioLabs). The cutting point is illustrated with an arrow and the binding region is orange shadowed in (B). M denotes 100 bp DNA ladder (#10488058, Invitrogen). Samples are described in (B). (E) Pan-primer set [ 21 ] used as reference for the detection of all Plasmodium species. TTP values are displayed in minutes and labelled.
Techniques Used: Sequencing, Amplification, Nucleic Acid Electrophoresis, Binding Assay
3) Product Images from "Genetic Polymorphism and Expression of CXCR4 in Breast Cancer"
Article Title: Genetic Polymorphism and Expression of CXCR4 in Breast Cancer
Journal: Analytical cellular pathology (Amsterdam)
doi: 10.1155/2015/289510
Figure Legend Snippet: CXCR4 rs2228014 (C/T) genetic polymorphism. (a) Electrophoretic profile of rs2228014 (C/T). BccI restriction enzyme was used for 4 h at 37°C. Polyacrylamide gel 10% stained with silver nitrate. Lane 1, Ladder DNA fragment marker of 100 bp; Lane 2, PCR product of 236 pb; Lane 3, wild-type homozygous genotype of 133 pb and 103 pb (CC); Lane 4, heterozygous genotype of 236 pb, 133 pb, and 103 pb (CT); Lane 5, blank reaction or negative control (reaction without DNA). (b) Genotype distribution for CXCR4 rs2228014 in breast cancer patients.
Techniques Used: Staining, Marker, Polymerase Chain Reaction, Negative Control
4) Product Images from "Quantitative and rapid Plasmodium falciparum malaria diagnosis and artemisinin-resistance detection using a CMOS Lab-on-Chip platform"
Article Title: Quantitative and rapid Plasmodium falciparum malaria diagnosis and artemisinin-resistance detection using a CMOS Lab-on-Chip platform
Journal: Biosensors & bioelectronics
doi: 10.1016/j.bios.2019.111678
![Sequence alignment, designed primers and analytical specificity for the detection of P. falciparum . (A) Illustration of the gene kelch 13 highlighting the location of the LAMP-PfK13 amplicon. (B) Alignment of all human-infective Plasmodium species with P. falciparum as reference sequence. Mismatches in the alignment are displayed as AGTC whereas matched nucleotides are represented as dots. The LAMP-PfK13 primers are illustrated on top of the alignment. (C) . (D) Results showing specific amplification of P. falciparum . The restriction enzyme used for this experiment is BccI (# R0704S, New England BioLabs). The cutting point is illustrated with an arrow and the binding region is orange shadowed in (B). M denotes 100 bp DNA ladder (#10488058, Invitrogen). Acronyms are described in (B). (E) ] used as control for the detection of all Plasmodium species. TTP values are displayed in minutes and labelled.](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7224984/bin/EMS86402-f003.jpg "... The restriction enzyme used for this experiment is BccI (# R0704S, New England BioLabs). The cutting point ...")
Figure Legend Snippet: Sequence alignment, designed primers and analytical specificity for the detection of P. falciparum . (A) Illustration of the gene kelch 13 highlighting the location of the LAMP-PfK13 amplicon. (B) Alignment of all human-infective Plasmodium species with P. falciparum as reference sequence. Mismatches in the alignment are displayed as AGTC whereas matched nucleotides are represented as dots. The LAMP-PfK13 primers are illustrated on top of the alignment. (C) . (D) Results showing specific amplification of P. falciparum . The restriction enzyme used for this experiment is BccI (# R0704S, New England BioLabs). The cutting point is illustrated with an arrow and the binding region is orange shadowed in (B). M denotes 100 bp DNA ladder (#10488058, Invitrogen). Acronyms are described in (B). (E) ] used as control for the detection of all Plasmodium species. TTP values are displayed in minutes and labelled.
Techniques Used: Sequencing, Amplification, Binding Assay
Related Articles
Amplification:Article Title: Quantitative and rapid Plasmodium falciparum malaria diagnosis and artemisinin-resistance detection using a CMOS Lab-on-Chip platform Article Snippet: .. Digested amplified products with the Article Title: Quantitative and rapid Plasmodium falciparum malaria diagnosis and artemisinin-resistance detection using a CMOS Lab-on-Chip platform Article Snippet: To further confirm specificity, a gel electrophoresis containing the post-amplification products is shown in . .. Digested amplified products with the Article Title: Kinesin Family Member 6 (kif6) Is Necessary for Spine Development in Zebrafish Article Snippet: WT embryos were collected and 40–50 pg of pooled TALENs were injected into the yolk of one- to four-cell stage embryos. .. Injected embryos and progeny were screened for TALEN-induced mutations by digesting PCR amplified DNA with Polymerase Chain Reaction:Article Title: Genetic Polymorphism and Expression of CXCR4 in Breast Cancer Article Snippet: Amplicons of 236 base pairs were analyzed by electrophoresis in 2% agarose gel and visualized using UV fluorescence after staining with Blue Green reagent (LGC Biotecnologia, Sao Paulo, Brazil). .. CXCR4 Genotyping PCR products were subjected to restriction digestion by incubating with Article Title: Distinct Vegfa isoforms control endothelial cell proliferation through PI3 kinase signalling mediated regulation of cdkn1a/p21 Article Snippet: .. Genotyping Primers for genotyping vegfaa were: Vegfaa-fwd: 5’ -GCTTTCTTAATTGTTTTGAGAGCCAG- 3’ Vegfaa-rev: 5’ –GGTGTGGGCTATTGCATTTC- 3’ PCR products were digested with Article Title: Screening for THAP1 Mutations in Polish Patients with Dystonia Shows Known and Novel Substitutions Article Snippet: Methods THAP1 exons 1, 2 and 3 including exon-intron boundaries and 5’UTR fragment were PCR-amplified and sequenced as described elsewhere [ ]. .. The presence of the identified mutations was additionally confirmed with PCR-RFLP method with the use of Hpy188I, New England BioLabs (Glu56Gly); SspI, Thermo Scientific (Ile80Val); and Article Title: Kinesin Family Member 6 (kif6) Is Necessary for Spine Development in Zebrafish Article Snippet: WT embryos were collected and 40–50 pg of pooled TALENs were injected into the yolk of one- to four-cell stage embryos. .. Injected embryos and progeny were screened for TALEN-induced mutations by digesting PCR amplified DNA with Agarose Gel Electrophoresis:Article Title: TNFSF15 promoter polymorphisms increase the susceptibility to small cell lung cancer: a case-control study Article Snippet: The target DNA fragment containing − 358 T > C (rs6478109) was amplified with primer pairs, − 358 -PF (5′-AAA TGT GAT TTC CGT TTC CCCA-3′) and − 358 -PR (5′- AAT ATA CCT GTT CCC TGC ACTG -3′). .. Briefly, PCR was performed using 6 μL reaction mixture containing 10 ng DNA, 0.1 μM each primer, and 1 × Taq PCR StarMix with loading dye (Genstar, Beijing, China).The PCR thermal cycling condition consists of an initial denaturation step at 94 °C for 3 min, followed by 30 cycles of 94 °C for 40 s, 58 °C for 30 s and 72 °C for 15 s, and then a final extension step at 72 °C for 3 min. PCR products for TNFSF15 –638A > G (114 bp) and -358 T > C (123 bp) were digested by Rsa I and Injection:Article Title: Kinesin Family Member 6 (kif6) Is Necessary for Spine Development in Zebrafish Article Snippet: WT embryos were collected and 40–50 pg of pooled TALENs were injected into the yolk of one- to four-cell stage embryos. .. Injected embryos and progeny were screened for TALEN-induced mutations by digesting PCR amplified DNA with |