bcci  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Name:
    BccI
    Description:
    BccI 5 000 units
    Catalog Number:
    R0704L
    Price:
    282
    Category:
    Restriction Enzymes
    Size:
    5 000 units
    Buy from Supplier


    Structured Review

    New England Biolabs bcci
    BccI
    BccI 5 000 units
    https://www.bioz.com/result/bcci/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bcci - by Bioz Stars, 2021-04
    94/100 stars

    Images

    1) Product Images from "Kinesin Family Member 6 (kif6) Is Necessary for Spine Development in Zebrafish"

    Article Title: Kinesin Family Member 6 (kif6) Is Necessary for Spine Development in Zebrafish

    Journal: Developmental dynamics : an official publication of the American Association of Anatomists

    doi: 10.1002/dvdy.24208

    Design and efficiency of kif6 TALENs. A: Genomic sequence showing left and right TALENs that were designed in the second exon of kif6 near the gw326 mutant allele. The TALEN pair flank a BccI restriction site. B: BccI restriction digest of PCR amplified
    Figure Legend Snippet: Design and efficiency of kif6 TALENs. A: Genomic sequence showing left and right TALENs that were designed in the second exon of kif6 near the gw326 mutant allele. The TALEN pair flank a BccI restriction site. B: BccI restriction digest of PCR amplified

    Techniques Used: TALENs, Sequencing, Mutagenesis, Polymerase Chain Reaction, Amplification

    2) Product Images from "Quantitative and rapid Plasmodium falciparum malaria diagnosis and artemisinin-resistance detection using a CMOS Lab-on-Chip platform"

    Article Title: Quantitative and rapid Plasmodium falciparum malaria diagnosis and artemisinin-resistance detection using a CMOS Lab-on-Chip platform

    Journal: bioRxiv

    doi: 10.1101/638221

    Sequence alignment, primer design and analytical specificity for the detection of P. falciparum . (A) Illustration of the gene kelch 13 showing in black the region selected for primer design. (B) Alignment of all human-infective Plasmodium species with P. falciparum as reference sequence. Mismatches in the alignment are displayed as AGTC whereas matched nucleotides are represented as dots. Primers are illustrated on top of the alignment. (C) Primer location in LAMP amplicon (5’ to 3’ direction). Sequences of the primers can be found in table S1. (D) Results showing specific amplification of P. falciparum DNA samples from clinical isolates as well as gel electrophoresis of the amplified products to confirm specificity. Digested amplification products are shown in fig. S2. The restriction enzyme used for this experiment is BccI (# R0704S, New England BioLabs). The cutting point is illustrated with an arrow and the binding region is orange shadowed in (B). M denotes 100 bp DNA ladder (#10488058, Invitrogen). Samples are described in (B). (E) Pan-primer set [ 21 ] used as reference for the detection of all Plasmodium species. TTP values are displayed in minutes and labelled.
    Figure Legend Snippet: Sequence alignment, primer design and analytical specificity for the detection of P. falciparum . (A) Illustration of the gene kelch 13 showing in black the region selected for primer design. (B) Alignment of all human-infective Plasmodium species with P. falciparum as reference sequence. Mismatches in the alignment are displayed as AGTC whereas matched nucleotides are represented as dots. Primers are illustrated on top of the alignment. (C) Primer location in LAMP amplicon (5’ to 3’ direction). Sequences of the primers can be found in table S1. (D) Results showing specific amplification of P. falciparum DNA samples from clinical isolates as well as gel electrophoresis of the amplified products to confirm specificity. Digested amplification products are shown in fig. S2. The restriction enzyme used for this experiment is BccI (# R0704S, New England BioLabs). The cutting point is illustrated with an arrow and the binding region is orange shadowed in (B). M denotes 100 bp DNA ladder (#10488058, Invitrogen). Samples are described in (B). (E) Pan-primer set [ 21 ] used as reference for the detection of all Plasmodium species. TTP values are displayed in minutes and labelled.

    Techniques Used: Sequencing, Amplification, Nucleic Acid Electrophoresis, Binding Assay

    3) Product Images from "Genetic Polymorphism and Expression of CXCR4 in Breast Cancer"

    Article Title: Genetic Polymorphism and Expression of CXCR4 in Breast Cancer

    Journal: Analytical cellular pathology (Amsterdam)

    doi: 10.1155/2015/289510

    CXCR4 rs2228014 (C/T) genetic polymorphism. (a) Electrophoretic profile of rs2228014 (C/T). BccI restriction enzyme was used for 4 h at 37°C. Polyacrylamide gel 10% stained with silver nitrate. Lane 1, Ladder DNA fragment marker of 100 bp; Lane 2, PCR product of 236 pb; Lane 3, wild-type homozygous genotype of 133 pb and 103 pb (CC); Lane 4, heterozygous genotype of 236 pb, 133 pb, and 103 pb (CT); Lane 5, blank reaction or negative control (reaction without DNA). (b) Genotype distribution for CXCR4 rs2228014 in breast cancer patients.
    Figure Legend Snippet: CXCR4 rs2228014 (C/T) genetic polymorphism. (a) Electrophoretic profile of rs2228014 (C/T). BccI restriction enzyme was used for 4 h at 37°C. Polyacrylamide gel 10% stained with silver nitrate. Lane 1, Ladder DNA fragment marker of 100 bp; Lane 2, PCR product of 236 pb; Lane 3, wild-type homozygous genotype of 133 pb and 103 pb (CC); Lane 4, heterozygous genotype of 236 pb, 133 pb, and 103 pb (CT); Lane 5, blank reaction or negative control (reaction without DNA). (b) Genotype distribution for CXCR4 rs2228014 in breast cancer patients.

    Techniques Used: Staining, Marker, Polymerase Chain Reaction, Negative Control

    4) Product Images from "Quantitative and rapid Plasmodium falciparum malaria diagnosis and artemisinin-resistance detection using a CMOS Lab-on-Chip platform"

    Article Title: Quantitative and rapid Plasmodium falciparum malaria diagnosis and artemisinin-resistance detection using a CMOS Lab-on-Chip platform

    Journal: Biosensors & bioelectronics

    doi: 10.1016/j.bios.2019.111678

    Sequence alignment, designed primers and analytical specificity for the detection of P. falciparum . (A) Illustration of the gene kelch 13 highlighting the location of the LAMP-PfK13 amplicon. (B) Alignment of all human-infective Plasmodium species with P. falciparum as reference sequence. Mismatches in the alignment are displayed as AGTC whereas matched nucleotides are represented as dots. The LAMP-PfK13 primers are illustrated on top of the alignment. (C) . (D) Results showing specific amplification of P. falciparum . The restriction enzyme used for this experiment is BccI (# R0704S, New England BioLabs). The cutting point is illustrated with an arrow and the binding region is orange shadowed in (B). M denotes 100 bp DNA ladder (#10488058, Invitrogen). Acronyms are described in (B). (E) ] used as control for the detection of all Plasmodium species. TTP values are displayed in minutes and labelled.
    Figure Legend Snippet: Sequence alignment, designed primers and analytical specificity for the detection of P. falciparum . (A) Illustration of the gene kelch 13 highlighting the location of the LAMP-PfK13 amplicon. (B) Alignment of all human-infective Plasmodium species with P. falciparum as reference sequence. Mismatches in the alignment are displayed as AGTC whereas matched nucleotides are represented as dots. The LAMP-PfK13 primers are illustrated on top of the alignment. (C) . (D) Results showing specific amplification of P. falciparum . The restriction enzyme used for this experiment is BccI (# R0704S, New England BioLabs). The cutting point is illustrated with an arrow and the binding region is orange shadowed in (B). M denotes 100 bp DNA ladder (#10488058, Invitrogen). Acronyms are described in (B). (E) ] used as control for the detection of all Plasmodium species. TTP values are displayed in minutes and labelled.

    Techniques Used: Sequencing, Amplification, Binding Assay

    Related Articles

    Amplification:

    Article Title: Quantitative and rapid Plasmodium falciparum malaria diagnosis and artemisinin-resistance detection using a CMOS Lab-on-Chip platform
    Article Snippet: .. Digested amplified products with the restriction enzyme BccI (#R0704S, New England Biolabs) are shown in fig. S2. ..

    Article Title: Quantitative and rapid Plasmodium falciparum malaria diagnosis and artemisinin-resistance detection using a CMOS Lab-on-Chip platform
    Article Snippet: To further confirm specificity, a gel electrophoresis containing the post-amplification products is shown in . .. Digested amplified products with the restriction enzyme BccI (#R0704S, New England Biolabs) are shown in . ..

    Article Title: Kinesin Family Member 6 (kif6) Is Necessary for Spine Development in Zebrafish
    Article Snippet: WT embryos were collected and 40–50 pg of pooled TALENs were injected into the yolk of one- to four-cell stage embryos. .. Injected embryos and progeny were screened for TALEN-induced mutations by digesting PCR amplified DNA with BccI (New England Biolabs). ..

    Polymerase Chain Reaction:

    Article Title: Genetic Polymorphism and Expression of CXCR4 in Breast Cancer
    Article Snippet: Amplicons of 236 base pairs were analyzed by electrophoresis in 2% agarose gel and visualized using UV fluorescence after staining with Blue Green reagent (LGC Biotecnologia, Sao Paulo, Brazil). .. CXCR4 Genotyping PCR products were subjected to restriction digestion by incubating with BccI (New England Biolabs, UK) for 4 h at 37°C. .. The enzymatic restriction products were analyzed by electrophoresis on 10% polyacrylamide gel and detected by a nonradioisotopic technique using silver staining.

    Article Title: Distinct Vegfa isoforms control endothelial cell proliferation through PI3 kinase signalling mediated regulation of cdkn1a/p21
    Article Snippet: .. Genotyping Primers for genotyping vegfaa were: Vegfaa-fwd: 5’ -GCTTTCTTAATTGTTTTGAGAGCCAG- 3’ Vegfaa-rev: 5’ –GGTGTGGGCTATTGCATTTC- 3’ PCR products were digested with BccI (NEB). ..

    Article Title: Screening for THAP1 Mutations in Polish Patients with Dystonia Shows Known and Novel Substitutions
    Article Snippet: Methods THAP1 exons 1, 2 and 3 including exon-intron boundaries and 5’UTR fragment were PCR-amplified and sequenced as described elsewhere [ ]. .. The presence of the identified mutations was additionally confirmed with PCR-RFLP method with the use of Hpy188I, New England BioLabs (Glu56Gly); SspI, Thermo Scientific (Ile80Val); and BccI, New England Biolabs (-249C > A) restriction enzymes. .. The significance of the coding substitutions was predicted with PolyPhen-2 HumVar model ( http://genetics.bwh.harvard.edu/pph2/ ) [ , ], SIFT ( http://sift.jcvi.org/ ) [ ] and Mutation Taster ( http://www.mutationtaster.org/ ) [ ] algorithms.

    Article Title: Kinesin Family Member 6 (kif6) Is Necessary for Spine Development in Zebrafish
    Article Snippet: WT embryos were collected and 40–50 pg of pooled TALENs were injected into the yolk of one- to four-cell stage embryos. .. Injected embryos and progeny were screened for TALEN-induced mutations by digesting PCR amplified DNA with BccI (New England Biolabs). ..

    Agarose Gel Electrophoresis:

    Article Title: TNFSF15 promoter polymorphisms increase the susceptibility to small cell lung cancer: a case-control study
    Article Snippet: The target DNA fragment containing − 358 T > C (rs6478109) was amplified with primer pairs, − 358 -PF (5′-AAA TGT GAT TTC CGT TTC CCCA-3′) and − 358 -PR (5′- AAT ATA CCT GTT CCC TGC ACTG -3′). .. Briefly, PCR was performed using 6 μL reaction mixture containing 10 ng DNA, 0.1 μM each primer, and 1 × Taq PCR StarMix with loading dye (Genstar, Beijing, China).The PCR thermal cycling condition consists of an initial denaturation step at 94 °C for 3 min, followed by 30 cycles of 94 °C for 40 s, 58 °C for 30 s and 72 °C for 15 s, and then a final extension step at 72 °C for 3 min. PCR products for TNFSF15 –638A > G (114 bp) and -358 T > C (123 bp) were digested by Rsa I and Bcc I (New England BioLabs, Inc., Beverly, USA) and separated on 3% agarose gel. .. The genotypes revealed by PCR-RFLP were further confirmed by DNA sequencing (Fig. ).

    Injection:

    Article Title: Kinesin Family Member 6 (kif6) Is Necessary for Spine Development in Zebrafish
    Article Snippet: WT embryos were collected and 40–50 pg of pooled TALENs were injected into the yolk of one- to four-cell stage embryos. .. Injected embryos and progeny were screened for TALEN-induced mutations by digesting PCR amplified DNA with BccI (New England Biolabs). ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    New England Biolabs bcci
    Sequence alignment, designed primers and analytical specificity for the detection of P. falciparum . (A) Illustration of the gene kelch 13 highlighting the location of the LAMP-PfK13 amplicon. (B) Alignment of all human-infective Plasmodium species with P. falciparum as reference sequence. Mismatches in the alignment are displayed as AGTC whereas matched nucleotides are represented as dots. The LAMP-PfK13 primers are illustrated on top of the alignment. (C) . (D) Results showing specific amplification of P. falciparum . The restriction enzyme used for this experiment is <t>BccI</t> (# <t>R0704S,</t> New England BioLabs). The cutting point is illustrated with an arrow and the binding region is orange shadowed in (B). M denotes 100 bp DNA ladder (#10488058, Invitrogen). Acronyms are described in (B). (E) ] used as control for the detection of all Plasmodium species. TTP values are displayed in minutes and labelled.
    Bcci, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bcci/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bcci - by Bioz Stars, 2021-04
    94/100 stars
      Buy from Supplier

    Image Search Results


    Sequence alignment, designed primers and analytical specificity for the detection of P. falciparum . (A) Illustration of the gene kelch 13 highlighting the location of the LAMP-PfK13 amplicon. (B) Alignment of all human-infective Plasmodium species with P. falciparum as reference sequence. Mismatches in the alignment are displayed as AGTC whereas matched nucleotides are represented as dots. The LAMP-PfK13 primers are illustrated on top of the alignment. (C) . (D) Results showing specific amplification of P. falciparum . The restriction enzyme used for this experiment is BccI (# R0704S, New England BioLabs). The cutting point is illustrated with an arrow and the binding region is orange shadowed in (B). M denotes 100 bp DNA ladder (#10488058, Invitrogen). Acronyms are described in (B). (E) ] used as control for the detection of all Plasmodium species. TTP values are displayed in minutes and labelled.

    Journal: Biosensors & bioelectronics

    Article Title: Quantitative and rapid Plasmodium falciparum malaria diagnosis and artemisinin-resistance detection using a CMOS Lab-on-Chip platform

    doi: 10.1016/j.bios.2019.111678

    Figure Lengend Snippet: Sequence alignment, designed primers and analytical specificity for the detection of P. falciparum . (A) Illustration of the gene kelch 13 highlighting the location of the LAMP-PfK13 amplicon. (B) Alignment of all human-infective Plasmodium species with P. falciparum as reference sequence. Mismatches in the alignment are displayed as AGTC whereas matched nucleotides are represented as dots. The LAMP-PfK13 primers are illustrated on top of the alignment. (C) . (D) Results showing specific amplification of P. falciparum . The restriction enzyme used for this experiment is BccI (# R0704S, New England BioLabs). The cutting point is illustrated with an arrow and the binding region is orange shadowed in (B). M denotes 100 bp DNA ladder (#10488058, Invitrogen). Acronyms are described in (B). (E) ] used as control for the detection of all Plasmodium species. TTP values are displayed in minutes and labelled.

    Article Snippet: Digested amplified products with the restriction enzyme BccI (#R0704S, New England Biolabs) are shown in .

    Techniques: Sequencing, Amplification, Binding Assay

    Sequence alignment, primer design and analytical specificity for the detection of P. falciparum . (A) Illustration of the gene kelch 13 showing in black the region selected for primer design. (B) Alignment of all human-infective Plasmodium species with P. falciparum as reference sequence. Mismatches in the alignment are displayed as AGTC whereas matched nucleotides are represented as dots. Primers are illustrated on top of the alignment. (C) Primer location in LAMP amplicon (5’ to 3’ direction). Sequences of the primers can be found in table S1. (D) Results showing specific amplification of P. falciparum DNA samples from clinical isolates as well as gel electrophoresis of the amplified products to confirm specificity. Digested amplification products are shown in fig. S2. The restriction enzyme used for this experiment is BccI (# R0704S, New England BioLabs). The cutting point is illustrated with an arrow and the binding region is orange shadowed in (B). M denotes 100 bp DNA ladder (#10488058, Invitrogen). Samples are described in (B). (E) Pan-primer set [ 21 ] used as reference for the detection of all Plasmodium species. TTP values are displayed in minutes and labelled.

    Journal: bioRxiv

    Article Title: Quantitative and rapid Plasmodium falciparum malaria diagnosis and artemisinin-resistance detection using a CMOS Lab-on-Chip platform

    doi: 10.1101/638221

    Figure Lengend Snippet: Sequence alignment, primer design and analytical specificity for the detection of P. falciparum . (A) Illustration of the gene kelch 13 showing in black the region selected for primer design. (B) Alignment of all human-infective Plasmodium species with P. falciparum as reference sequence. Mismatches in the alignment are displayed as AGTC whereas matched nucleotides are represented as dots. Primers are illustrated on top of the alignment. (C) Primer location in LAMP amplicon (5’ to 3’ direction). Sequences of the primers can be found in table S1. (D) Results showing specific amplification of P. falciparum DNA samples from clinical isolates as well as gel electrophoresis of the amplified products to confirm specificity. Digested amplification products are shown in fig. S2. The restriction enzyme used for this experiment is BccI (# R0704S, New England BioLabs). The cutting point is illustrated with an arrow and the binding region is orange shadowed in (B). M denotes 100 bp DNA ladder (#10488058, Invitrogen). Samples are described in (B). (E) Pan-primer set [ 21 ] used as reference for the detection of all Plasmodium species. TTP values are displayed in minutes and labelled.

    Article Snippet: Digested amplified products with the restriction enzyme BccI (#R0704S, New England Biolabs) are shown in fig. S2.

    Techniques: Sequencing, Amplification, Nucleic Acid Electrophoresis, Binding Assay

    CXCR4 rs2228014 (C/T) genetic polymorphism. (a) Electrophoretic profile of rs2228014 (C/T). BccI restriction enzyme was used for 4 h at 37°C. Polyacrylamide gel 10% stained with silver nitrate. Lane 1, Ladder DNA fragment marker of 100 bp; Lane 2, PCR product of 236 pb; Lane 3, wild-type homozygous genotype of 133 pb and 103 pb (CC); Lane 4, heterozygous genotype of 236 pb, 133 pb, and 103 pb (CT); Lane 5, blank reaction or negative control (reaction without DNA). (b) Genotype distribution for CXCR4 rs2228014 in breast cancer patients.

    Journal: Analytical cellular pathology (Amsterdam)

    Article Title: Genetic Polymorphism and Expression of CXCR4 in Breast Cancer

    doi: 10.1155/2015/289510

    Figure Lengend Snippet: CXCR4 rs2228014 (C/T) genetic polymorphism. (a) Electrophoretic profile of rs2228014 (C/T). BccI restriction enzyme was used for 4 h at 37°C. Polyacrylamide gel 10% stained with silver nitrate. Lane 1, Ladder DNA fragment marker of 100 bp; Lane 2, PCR product of 236 pb; Lane 3, wild-type homozygous genotype of 133 pb and 103 pb (CC); Lane 4, heterozygous genotype of 236 pb, 133 pb, and 103 pb (CT); Lane 5, blank reaction or negative control (reaction without DNA). (b) Genotype distribution for CXCR4 rs2228014 in breast cancer patients.

    Article Snippet: CXCR4 Genotyping PCR products were subjected to restriction digestion by incubating with BccI (New England Biolabs, UK) for 4 h at 37°C.

    Techniques: Staining, Marker, Polymerase Chain Reaction, Negative Control

    Design and efficiency of kif6 TALENs. A: Genomic sequence showing left and right TALENs that were designed in the second exon of kif6 near the gw326 mutant allele. The TALEN pair flank a BccI restriction site. B: BccI restriction digest of PCR amplified

    Journal: Developmental dynamics : an official publication of the American Association of Anatomists

    Article Title: Kinesin Family Member 6 (kif6) Is Necessary for Spine Development in Zebrafish

    doi: 10.1002/dvdy.24208

    Figure Lengend Snippet: Design and efficiency of kif6 TALENs. A: Genomic sequence showing left and right TALENs that were designed in the second exon of kif6 near the gw326 mutant allele. The TALEN pair flank a BccI restriction site. B: BccI restriction digest of PCR amplified

    Article Snippet: Injected embryos and progeny were screened for TALEN-induced mutations by digesting PCR amplified DNA with BccI (New England Biolabs).

    Techniques: TALENs, Sequencing, Mutagenesis, Polymerase Chain Reaction, Amplification