bax antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc bax antibody
    Bax Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bax antibody/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bax antibody - by Bioz Stars, 2023-09
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    antibodies against bax  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc antibodies against bax
    Antibodies Against Bax, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against bax/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    α bax  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc α bax
    α Bax, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α bax/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    α bax - by Bioz Stars, 2023-09
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    antibodies against bax  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc antibodies against bax
    RSV protects against BUP-induced apoptosis via the upregulation of SIRT1 expression. (A) Representative western blot images and semi-quantification of <t>Bax,</t> <t>Bcl-2</t> and cleaved caspase-3 protein levels in the control, BUP, RSV + BUP and EX527 + RSV + BUP groups. Q1 (upper-left quadrant) represents dead cells; Q2 (upper-right quadrant) represents late apoptotic cells; Q3 (lower-right quadrant) represents early apoptotic cells; Q4 (lower-left quadrant) represents live cells. (B) Representative images of the flow cytometric analysis of Annexin V/7-AAD staining and quantification of the apoptotic rates in each group. Data are presented as the mean ± SEM (n=3). * P<0.05 vs. the control group; # P<0.05 vs. the BUP group; & P<0.05 vs. the RSV + BUP group. RSV, resveratrol; BUP, bupivacaine; SIRT1, sirtuin 1; EX527, SIRT1 inhibitor; 7-AAD, 7-aminoactinomycin D.
    Antibodies Against Bax, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against bax/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    antibodies against bax - by Bioz Stars, 2023-09
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    Images

    1) Product Images from "Resveratrol‑mediated activation of SIRT1 inhibits the PERK‑eIF2α‑ATF4 pathway and mitigates bupivacaine‑induced neurotoxicity in PC12 cells"

    Article Title: Resveratrol‑mediated activation of SIRT1 inhibits the PERK‑eIF2α‑ATF4 pathway and mitigates bupivacaine‑induced neurotoxicity in PC12 cells

    Journal: Experimental and Therapeutic Medicine

    doi: 10.3892/etm.2023.12132

    RSV protects against BUP-induced apoptosis via the upregulation of SIRT1 expression. (A) Representative western blot images and semi-quantification of Bax, Bcl-2 and cleaved caspase-3 protein levels in the control, BUP, RSV + BUP and EX527 + RSV + BUP groups. Q1 (upper-left quadrant) represents dead cells; Q2 (upper-right quadrant) represents late apoptotic cells; Q3 (lower-right quadrant) represents early apoptotic cells; Q4 (lower-left quadrant) represents live cells. (B) Representative images of the flow cytometric analysis of Annexin V/7-AAD staining and quantification of the apoptotic rates in each group. Data are presented as the mean ± SEM (n=3). * P<0.05 vs. the control group; # P<0.05 vs. the BUP group; & P<0.05 vs. the RSV + BUP group. RSV, resveratrol; BUP, bupivacaine; SIRT1, sirtuin 1; EX527, SIRT1 inhibitor; 7-AAD, 7-aminoactinomycin D.
    Figure Legend Snippet: RSV protects against BUP-induced apoptosis via the upregulation of SIRT1 expression. (A) Representative western blot images and semi-quantification of Bax, Bcl-2 and cleaved caspase-3 protein levels in the control, BUP, RSV + BUP and EX527 + RSV + BUP groups. Q1 (upper-left quadrant) represents dead cells; Q2 (upper-right quadrant) represents late apoptotic cells; Q3 (lower-right quadrant) represents early apoptotic cells; Q4 (lower-left quadrant) represents live cells. (B) Representative images of the flow cytometric analysis of Annexin V/7-AAD staining and quantification of the apoptotic rates in each group. Data are presented as the mean ± SEM (n=3). * P<0.05 vs. the control group; # P<0.05 vs. the BUP group; & P<0.05 vs. the RSV + BUP group. RSV, resveratrol; BUP, bupivacaine; SIRT1, sirtuin 1; EX527, SIRT1 inhibitor; 7-AAD, 7-aminoactinomycin D.

    Techniques Used: Expressing, Western Blot, Staining

    RSV ameliorates BUP-induced apoptosis by inhibiting the PERK pathway. (A) Representative western blot images and semi-quantification of Bax, Bcl-2 and cleaved caspase-3 protein levels in the control, BUP, RSV + BUP and CCT + RSV + BUP groups. Q1 (upper-left quadrant) represents dead cells; Q2 (upper-right quadrant) represents late apoptotic cells; Q3 (lower-right quadrant) represents early apoptotic cells; Q4 (lower-left quadrant) represents live cells. (B) Representative images of the flow cytometric analysis of Annexin V/7-AAD staining and quantification of the apoptotic rates in each group. Data are presented as the mean ± SEM (n=3). * P<0.05 vs. the control group; # P<0.05 vs. the BUP group; & P<0.05 vs. the RSV + BUP group. RSV, resveratrol; BUP, bupivacaine; CCT, CCT020312 (PERK activator); PERK, protein kinase RNA-like ER kinase; 7-AAD, 7-aminoactinomycin D.
    Figure Legend Snippet: RSV ameliorates BUP-induced apoptosis by inhibiting the PERK pathway. (A) Representative western blot images and semi-quantification of Bax, Bcl-2 and cleaved caspase-3 protein levels in the control, BUP, RSV + BUP and CCT + RSV + BUP groups. Q1 (upper-left quadrant) represents dead cells; Q2 (upper-right quadrant) represents late apoptotic cells; Q3 (lower-right quadrant) represents early apoptotic cells; Q4 (lower-left quadrant) represents live cells. (B) Representative images of the flow cytometric analysis of Annexin V/7-AAD staining and quantification of the apoptotic rates in each group. Data are presented as the mean ± SEM (n=3). * P<0.05 vs. the control group; # P<0.05 vs. the BUP group; & P<0.05 vs. the RSV + BUP group. RSV, resveratrol; BUP, bupivacaine; CCT, CCT020312 (PERK activator); PERK, protein kinase RNA-like ER kinase; 7-AAD, 7-aminoactinomycin D.

    Techniques Used: Western Blot, Staining

    bax antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc bax antibody
    ( A ) (Left) Bar graphs depicting fold changes in cell numbers of H23 and H1792 cells ectopically expressing BCL2L1 (BCL-xL), BCL2 , or MCL1 cDNAs treated for 72 hours with combined 25 nM eFT226 (eIF4Ai) and 100 nM MRTX849 (KRASi). Data are represented as means ± SD. n = 3. (Right) Immunoblots confirming overexpression of BCL-xL, BCL-2, and MCL1 by cDNAs. ( B ) Immunoblots showing interactions of MCL1 and BCL-xL with immunoprecipitated <t>proapoptotic</t> <t>BIM</t> and <t>BAX</t> proteins, respectively, in response to specified drug treatments in sensitive H23 cells.
    Bax Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bax antibody/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bax antibody - by Bioz Stars, 2023-09
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    Images

    1) Product Images from "Cotargeting a MYC/eIF4A-survival axis improves the efficacy of KRAS inhibitors in lung cancer"

    Article Title: Cotargeting a MYC/eIF4A-survival axis improves the efficacy of KRAS inhibitors in lung cancer

    Journal: The Journal of Clinical Investigation

    doi: 10.1172/JCI167651

    ( A ) (Left) Bar graphs depicting fold changes in cell numbers of H23 and H1792 cells ectopically expressing BCL2L1 (BCL-xL), BCL2 , or MCL1 cDNAs treated for 72 hours with combined 25 nM eFT226 (eIF4Ai) and 100 nM MRTX849 (KRASi). Data are represented as means ± SD. n = 3. (Right) Immunoblots confirming overexpression of BCL-xL, BCL-2, and MCL1 by cDNAs. ( B ) Immunoblots showing interactions of MCL1 and BCL-xL with immunoprecipitated proapoptotic BIM and BAX proteins, respectively, in response to specified drug treatments in sensitive H23 cells.
    Figure Legend Snippet: ( A ) (Left) Bar graphs depicting fold changes in cell numbers of H23 and H1792 cells ectopically expressing BCL2L1 (BCL-xL), BCL2 , or MCL1 cDNAs treated for 72 hours with combined 25 nM eFT226 (eIF4Ai) and 100 nM MRTX849 (KRASi). Data are represented as means ± SD. n = 3. (Right) Immunoblots confirming overexpression of BCL-xL, BCL-2, and MCL1 by cDNAs. ( B ) Immunoblots showing interactions of MCL1 and BCL-xL with immunoprecipitated proapoptotic BIM and BAX proteins, respectively, in response to specified drug treatments in sensitive H23 cells.

    Techniques Used: Expressing, Western Blot, Over Expression, Immunoprecipitation

    ( A ) Immunoblots showing MCL1, BCL-xL, and BCL-2 protein levels in response to 24 hours of specified treatments in control and MYC-overexpressing resistant lines. ( B and C ) Immunoblots showing interactions of MCL1 and BCL-xL with immunoprecipitated proapoptotic BIM and BAX proteins, respectively, in response to specified drug treatments in control and MYC-overexpressing resistant lines. The GAPDH immunoblots denoted by asterisks, which serve as a loading control, have been duplicated from the left side of that panel, because immunoblots were all generated from the same gel and membrane. (Right) Immunoblots confirm ectopic expression of MYC by cDNAs.
    Figure Legend Snippet: ( A ) Immunoblots showing MCL1, BCL-xL, and BCL-2 protein levels in response to 24 hours of specified treatments in control and MYC-overexpressing resistant lines. ( B and C ) Immunoblots showing interactions of MCL1 and BCL-xL with immunoprecipitated proapoptotic BIM and BAX proteins, respectively, in response to specified drug treatments in control and MYC-overexpressing resistant lines. The GAPDH immunoblots denoted by asterisks, which serve as a loading control, have been duplicated from the left side of that panel, because immunoblots were all generated from the same gel and membrane. (Right) Immunoblots confirm ectopic expression of MYC by cDNAs.

    Techniques Used: Western Blot, Immunoprecipitation, Generated, Expressing

    rabbit anti human bax  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti human bax
    YY1 knockdown induces apoptosis in HL-60 cells. ( A ) Flow cytometry plots showing Annexin V and propidium iodide (PI) double staining in HL-60-sh-doxy (control) and sh-doxy-YY1 cells untreated and treated with 1 μM ATRA for 72 h and 96 h. ( B ) Western blots <t>detecting</t> <t>PARP1,</t> <t>BAX,</t> and caspase 3 protein levels in HL-60-sh-doxy (control) and HL-60-YY1 sh-YY1-doxy cells.
    Rabbit Anti Human Bax, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti human bax/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    rabbit anti human bax - by Bioz Stars, 2023-09
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    Images

    1) Product Images from "YY1 Knockdown Relieves the Differentiation Block and Restores Apoptosis in AML Cells"

    Article Title: YY1 Knockdown Relieves the Differentiation Block and Restores Apoptosis in AML Cells

    Journal: Cancers

    doi: 10.3390/cancers15154010

    YY1 knockdown induces apoptosis in HL-60 cells. ( A ) Flow cytometry plots showing Annexin V and propidium iodide (PI) double staining in HL-60-sh-doxy (control) and sh-doxy-YY1 cells untreated and treated with 1 μM ATRA for 72 h and 96 h. ( B ) Western blots detecting PARP1, BAX, and caspase 3 protein levels in HL-60-sh-doxy (control) and HL-60-YY1 sh-YY1-doxy cells.
    Figure Legend Snippet: YY1 knockdown induces apoptosis in HL-60 cells. ( A ) Flow cytometry plots showing Annexin V and propidium iodide (PI) double staining in HL-60-sh-doxy (control) and sh-doxy-YY1 cells untreated and treated with 1 μM ATRA for 72 h and 96 h. ( B ) Western blots detecting PARP1, BAX, and caspase 3 protein levels in HL-60-sh-doxy (control) and HL-60-YY1 sh-YY1-doxy cells.

    Techniques Used: Flow Cytometry, Double Staining, Western Blot

    resource source identifier antibodies bax cell signaling technology  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc resource source identifier antibodies bax cell signaling technology
    Resource Source Identifier Antibodies Bax Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/resource source identifier antibodies bax cell signaling technology/product/Cell Signaling Technology Inc
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    resource source identifier antibodies bax cell signaling technology - by Bioz Stars, 2023-09
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    anti bax 2772 antibodies  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti bax 2772 antibodies
    Anti Bax 2772 Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    x protein bax  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc x protein bax
    X Protein Bax, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    x protein bax - by Bioz Stars, 2023-09
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    bax antibody  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc bax antibody
    a Superposition of <t>the</t> <t>BCL-2</t> structure in the p53-DBD/BCL-2 complex with the BCL-2 structure in the <t>BCL-2/Bax-BH3</t> complex (PDB: 2XA0 ). BCL-2 and p53 in the p53-DBD/BCL-2 complex are colored hot pink and green, respectively. BCL-2 and Bax-BH3 in the BCL-2/Bax-BH3 complex are colored pink and light blue, respectively. b Superposition of BCL-2 structures shows the BH3-binding pockets. c , d The electrostatic surface potential of the BCL-2 molecule shows BCL-2/Bax-BH3 interactions ( c ) or BCL-2/p53-DBD interactions ( d ). The black box indicates the hydrophobic pockets of BCL-2. e , f The interfacial BCL-2 residues in the BCL-2/Bax-BH3 structure ( e ) or BCL-2/p53-DBD structure ( f ). Residues are colored and labelled as indicated.
    Bax Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bax antibody/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bax antibody - by Bioz Stars, 2023-09
    86/100 stars

    Images

    1) Product Images from "Structures of p53/BCL-2 complex suggest a mechanism for p53 to antagonize BCL-2 activity"

    Article Title: Structures of p53/BCL-2 complex suggest a mechanism for p53 to antagonize BCL-2 activity

    Journal: Nature Communications

    doi: 10.1038/s41467-023-40087-2

    a Superposition of the BCL-2 structure in the p53-DBD/BCL-2 complex with the BCL-2 structure in the BCL-2/Bax-BH3 complex (PDB: 2XA0 ). BCL-2 and p53 in the p53-DBD/BCL-2 complex are colored hot pink and green, respectively. BCL-2 and Bax-BH3 in the BCL-2/Bax-BH3 complex are colored pink and light blue, respectively. b Superposition of BCL-2 structures shows the BH3-binding pockets. c , d The electrostatic surface potential of the BCL-2 molecule shows BCL-2/Bax-BH3 interactions ( c ) or BCL-2/p53-DBD interactions ( d ). The black box indicates the hydrophobic pockets of BCL-2. e , f The interfacial BCL-2 residues in the BCL-2/Bax-BH3 structure ( e ) or BCL-2/p53-DBD structure ( f ). Residues are colored and labelled as indicated.
    Figure Legend Snippet: a Superposition of the BCL-2 structure in the p53-DBD/BCL-2 complex with the BCL-2 structure in the BCL-2/Bax-BH3 complex (PDB: 2XA0 ). BCL-2 and p53 in the p53-DBD/BCL-2 complex are colored hot pink and green, respectively. BCL-2 and Bax-BH3 in the BCL-2/Bax-BH3 complex are colored pink and light blue, respectively. b Superposition of BCL-2 structures shows the BH3-binding pockets. c , d The electrostatic surface potential of the BCL-2 molecule shows BCL-2/Bax-BH3 interactions ( c ) or BCL-2/p53-DBD interactions ( d ). The black box indicates the hydrophobic pockets of BCL-2. e , f The interfacial BCL-2 residues in the BCL-2/Bax-BH3 structure ( e ) or BCL-2/p53-DBD structure ( f ). Residues are colored and labelled as indicated.

    Techniques Used: Binding Assay

    a Fluorescence polarization assay shows BCL-2 binding to fluorescein-labelled Bax-BH3 peptide (fBax-BH3). b Competitive inhibition assay of the p53-DBD to BCL-2/fBax-BH3 interaction. p53-DBD or its mutant was used as a competitor in the BCL-2/fBax-BH3 fluorescence polarization assay. The EC50 and IC50 values of ( a ) and ( b ) were determined by fitting the data using a sigmoidal dose-response nonlinear regression model and shown as indicated. Data are represented as the mean ± SEM of n = 4 independent experiments. c GST pull-down assay analyzing the effect of BH3-peptides on the interactions of GST-p53-DBD with His-BCL-2. The BH3 peptides are used as indicated. d , e GST pull-down assay analyzing the effect of p53-DBD on the interactions of GST-BCL-2 with Bax protein ( d ) or Bak protein ( e ). The source data are provided as a Source Data file.
    Figure Legend Snippet: a Fluorescence polarization assay shows BCL-2 binding to fluorescein-labelled Bax-BH3 peptide (fBax-BH3). b Competitive inhibition assay of the p53-DBD to BCL-2/fBax-BH3 interaction. p53-DBD or its mutant was used as a competitor in the BCL-2/fBax-BH3 fluorescence polarization assay. The EC50 and IC50 values of ( a ) and ( b ) were determined by fitting the data using a sigmoidal dose-response nonlinear regression model and shown as indicated. Data are represented as the mean ± SEM of n = 4 independent experiments. c GST pull-down assay analyzing the effect of BH3-peptides on the interactions of GST-p53-DBD with His-BCL-2. The BH3 peptides are used as indicated. d , e GST pull-down assay analyzing the effect of p53-DBD on the interactions of GST-BCL-2 with Bax protein ( d ) or Bak protein ( e ). The source data are provided as a Source Data file.

    Techniques Used: Fluorescence, Binding Assay, Inhibition, Mutagenesis, Pull Down Assay

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    • bax antibody  (Cell Signaling Technology Inc)
    86
    Cell Signaling Technology Inc bax antibody
    Bax Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bax antibody/product/Cell Signaling Technology Inc
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    antibodies against bax  (Cell Signaling Technology Inc)
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    Antibodies Against Bax, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    α bax  (Cell Signaling Technology Inc)
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    α Bax, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti human bax  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc rabbit anti human bax
    YY1 knockdown induces apoptosis in HL-60 cells. ( A ) Flow cytometry plots showing Annexin V and propidium iodide (PI) double staining in HL-60-sh-doxy (control) and sh-doxy-YY1 cells untreated and treated with 1 μM ATRA for 72 h and 96 h. ( B ) Western blots <t>detecting</t> <t>PARP1,</t> <t>BAX,</t> and caspase 3 protein levels in HL-60-sh-doxy (control) and HL-60-YY1 sh-YY1-doxy cells.
    Rabbit Anti Human Bax, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    resource source identifier antibodies bax cell signaling technology  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc resource source identifier antibodies bax cell signaling technology
    YY1 knockdown induces apoptosis in HL-60 cells. ( A ) Flow cytometry plots showing Annexin V and propidium iodide (PI) double staining in HL-60-sh-doxy (control) and sh-doxy-YY1 cells untreated and treated with 1 μM ATRA for 72 h and 96 h. ( B ) Western blots <t>detecting</t> <t>PARP1,</t> <t>BAX,</t> and caspase 3 protein levels in HL-60-sh-doxy (control) and HL-60-YY1 sh-YY1-doxy cells.
    Resource Source Identifier Antibodies Bax Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/resource source identifier antibodies bax cell signaling technology/product/Cell Signaling Technology Inc
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    anti bax 2772 antibodies  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc anti bax 2772 antibodies
    YY1 knockdown induces apoptosis in HL-60 cells. ( A ) Flow cytometry plots showing Annexin V and propidium iodide (PI) double staining in HL-60-sh-doxy (control) and sh-doxy-YY1 cells untreated and treated with 1 μM ATRA for 72 h and 96 h. ( B ) Western blots <t>detecting</t> <t>PARP1,</t> <t>BAX,</t> and caspase 3 protein levels in HL-60-sh-doxy (control) and HL-60-YY1 sh-YY1-doxy cells.
    Anti Bax 2772 Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti bax 2772 antibodies/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti bax 2772 antibodies - by Bioz Stars, 2023-09
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    x protein bax  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc x protein bax
    YY1 knockdown induces apoptosis in HL-60 cells. ( A ) Flow cytometry plots showing Annexin V and propidium iodide (PI) double staining in HL-60-sh-doxy (control) and sh-doxy-YY1 cells untreated and treated with 1 μM ATRA for 72 h and 96 h. ( B ) Western blots <t>detecting</t> <t>PARP1,</t> <t>BAX,</t> and caspase 3 protein levels in HL-60-sh-doxy (control) and HL-60-YY1 sh-YY1-doxy cells.
    X Protein Bax, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/x protein bax/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    x protein bax - by Bioz Stars, 2023-09
    86/100 stars
    		  Buy from Supplier

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    YY1 knockdown induces apoptosis in HL-60 cells. ( A ) Flow cytometry plots showing Annexin V and propidium iodide (PI) double staining in HL-60-sh-doxy (control) and sh-doxy-YY1 cells untreated and treated with 1 μM ATRA for 72 h and 96 h. ( B ) Western blots detecting PARP1, BAX, and caspase 3 protein levels in HL-60-sh-doxy (control) and HL-60-YY1 sh-YY1-doxy cells.

    Journal: Cancers

    Article Title: YY1 Knockdown Relieves the Differentiation Block and Restores Apoptosis in AML Cells

    doi: 10.3390/cancers15154010

    Figure Lengend Snippet: YY1 knockdown induces apoptosis in HL-60 cells. ( A ) Flow cytometry plots showing Annexin V and propidium iodide (PI) double staining in HL-60-sh-doxy (control) and sh-doxy-YY1 cells untreated and treated with 1 μM ATRA for 72 h and 96 h. ( B ) Western blots detecting PARP1, BAX, and caspase 3 protein levels in HL-60-sh-doxy (control) and HL-60-YY1 sh-YY1-doxy cells.

    Article Snippet: The following primary antibodies and dilutions were used for immunoblotting detection: rabbit anti-human YY1 (1:600, sc-1703, Santa-Cruz Biotechnology; Dallas, TX, USA); rabbit anti-human PARP1 (1:4000, ALX-210-302-R100, Life Sciences); rabbit anti-human BAX (1:200, #2772, Cell Signaling Technology; Danvers, MA, USA); rabbit anti-human caspase-3 (1:800, #9662, Cell Signaling Technology); rabbit anti-human C/EBPα (1:800, EP709Y-ab40764, Abcam; Cambridge, MA, USA); rabbit anti-human C/EBPδ (1:500, EPR23518-259-ab245214, Abcam); and rabbit anti-human C/EBPε (0.6 μg/mL, ab246861, Abcam).

    Techniques: Flow Cytometry, Double Staining, Western Blot