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(A) Sketched representation of the <t>proposed</t> <t>SiMoA</t> assay to <t>detect</t> <t>EV–LP</t> binding. (B) Control tests to assess nonspecific signals for HDL. (C) Control tests to assess nonspecific signals for LDL. (D) Control tests to assess nonspecific signals for VLDL. for all the graphs. Data are expressed in Average Enzyme per Bead (AEB). n = 2 independent experimental replicates. Legend: REV: unlabeled Red Blood Cell-derived EVs HDL: unlabeled High Density Lipoproteins. LDL: unlabeled Low Density Lipoproteins. VLDL: unlabeled Very Low Density Lipoproteins.
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(A) Sketched representation of the proposed SiMoA assay to detect EV–LP binding. (B) Control tests to assess nonspecific signals for HDL. (C) Control tests to assess nonspecific signals for LDL. (D) Control tests to assess nonspecific signals for VLDL. for all the graphs. Data are expressed in Average Enzyme per Bead (AEB). n = 2 independent experimental replicates. Legend: REV: unlabeled Red Blood Cell-derived EVs HDL: unlabeled High Density Lipoproteins. LDL: unlabeled Low Density Lipoproteins. VLDL: unlabeled Very Low Density Lipoproteins.

Journal: Analytical Chemistry

Article Title: Orthogonal Investigation at Single-Particle and Ensemble Levels Uncovers Lipoprotein-Extracellular Vesicle Binding

doi: 10.1021/acs.analchem.5c05327

Figure Lengend Snippet: (A) Sketched representation of the proposed SiMoA assay to detect EV–LP binding. (B) Control tests to assess nonspecific signals for HDL. (C) Control tests to assess nonspecific signals for LDL. (D) Control tests to assess nonspecific signals for VLDL. for all the graphs. Data are expressed in Average Enzyme per Bead (AEB). n = 2 independent experimental replicates. Legend: REV: unlabeled Red Blood Cell-derived EVs HDL: unlabeled High Density Lipoproteins. LDL: unlabeled Low Density Lipoproteins. VLDL: unlabeled Very Low Density Lipoproteins.

Article Snippet: The three-step SiMoA assay was performed using anti-Band 3-conjugated beads (Santa Cruz, sc-133190), unlabeled EV–LP complex samples, and biotinylated detector antibodies either anti-ApoA1 (Sigma-Aldrich, JS-251S1) or anti-ApoB100 (Santa Cruz, sc-393636).

Techniques: Binding Assay, Control, Derivative Assay