bamhi  (Jena Bioscience)


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  • 93
    Name:
    BamHI
    Description:

    Catalog Number:
    EN-103L
    Price:
    120.5
    Category:
    Molecular Biology
    Size:
    5 x 7500 Units
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    Structured Review

    Jena Bioscience bamhi

    https://www.bioz.com/result/bamhi/product/Jena Bioscience
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bamhi - by Bioz Stars, 2021-06
    93/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Chromatin-associated protein complexes link DNA base J and transcription termination in Leishmania
    Article Snippet: Plasmid construction To create an expression vector that expresses epitope-tagged transgenes in Leishmania , the MHTAP tag (which bears a Myc epitope, six histidines, a protein A domain, and calmodulin binding peptide) was amplified from the plasmid pLEW-MHTAP ( ) with the primers MHTAP-BamHI-S and MHTAP-Not1-AS (all primers use in this study are described in Supplementary table S10). .. Following cleavage with BamHI and NotI, the PCR fragment was inserted into BglII+Not1-digested pLEXSY-I-bleCherry3 (Jena Biosciences). .. The resulting plasmid (pLEXSY-MHTAP) allows the TAP-tagging of introduced coding regions under the control of a Tet-regulated T7 promoter, and insertion into the ODC locus on chromosome 12 of L. tarentolae .

    Recombinant:

    Article Title: High protection of mice against Brucella abortus by oral immunization with recombinant probiotic Lactobacillus casei vector vaccine, expressing the outer membrane protein OMP19 of Brucella species.
    Article Snippet: Brucellosis is a zoonotic disease threatening the public health and hindering the trade of animals and their products, which has a negative impact on the economic development of a country. .. Brucellosis is a zoonotic disease threatening the public health and hindering the trade of animals and their products, which has a negative impact on the economic development of a country. .. Brucellosis is a zoonotic disease threatening the public health and hindering the trade of animals and their products, which has a negative impact on the economic development of a country.

    Plasmid Preparation:

    Article Title: High protection of mice against Brucella abortus by oral immunization with recombinant probiotic Lactobacillus casei vector vaccine, expressing the outer membrane protein OMP19 of Brucella species.
    Article Snippet: Brucellosis is a zoonotic disease threatening the public health and hindering the trade of animals and their products, which has a negative impact on the economic development of a country. .. Brucellosis is a zoonotic disease threatening the public health and hindering the trade of animals and their products, which has a negative impact on the economic development of a country. .. Brucellosis is a zoonotic disease threatening the public health and hindering the trade of animals and their products, which has a negative impact on the economic development of a country.

    Article Title: Fatty acid and retinol-binding protein: A novel antigen for immunodiagnosis of human strongyloidiasis
    Article Snippet: White colonies harboring the target construct was selected for enzymatic digestion. .. The vector was digested with BamHI and XhoI (Jena Bioscience, Jena, Germany) and the insert was ligated into the pET28a(+) expression vector (Novagen, USA), and was used for transformation of E . coli DH5α. .. The desired plasmid was purified by a commercial plasmid purification kit (Yekta Tajhiz Azma, Tehran, Iran) and transformed into E . coli BL21(DE3) for optimal expression of recombinant sSFAR (rSsFAR).

    Article Title: Chromatin-associated protein complexes link DNA base J and transcription termination in Leishmania
    Article Snippet: The availability of modern genome-wide approaches will no doubt provide the appropriate tools to answer these questions. .. Plasmid construction To create an expression vector that expresses epitope-tagged transgenes in Leishmania , the MHTAP tag (which bears a Myc epitope, six histidines, a protein A domain, and calmodulin binding peptide) was amplified from the plasmid pLEW-MHTAP ( ) with the primers MHTAP-BamHI-S and MHTAP-Not1-AS (all primers use in this study are described in Supplementary table S10). .. Following cleavage with BamHI and NotI, the PCR fragment was inserted into BglII+Not1-digested pLEXSY-I-bleCherry3 (Jena Biosciences).

    Article Title: Orally administered recombinant Lactobacillus casei vector vaccine expressing β-toxoid of Clostridium perfringens that induced protective immunity responses.
    Article Snippet: Clostridium perfringens types B and C cause enteritis and enterotoxemia in animals. .. Clostridium perfringens types B and C cause enteritis and enterotoxemia in animals. .. Clostridium perfringens types B and C cause enteritis and enterotoxemia in animals.

    Amplification:

    Article Title: High protection of mice against Brucella abortus by oral immunization with recombinant probiotic Lactobacillus casei vector vaccine, expressing the outer membrane protein OMP19 of Brucella species.
    Article Snippet: Brucellosis is a zoonotic disease threatening the public health and hindering the trade of animals and their products, which has a negative impact on the economic development of a country. .. Brucellosis is a zoonotic disease threatening the public health and hindering the trade of animals and their products, which has a negative impact on the economic development of a country. .. Brucellosis is a zoonotic disease threatening the public health and hindering the trade of animals and their products, which has a negative impact on the economic development of a country.

    Article Title: Chromatin-associated protein complexes link DNA base J and transcription termination in Leishmania
    Article Snippet: The availability of modern genome-wide approaches will no doubt provide the appropriate tools to answer these questions. .. Plasmid construction To create an expression vector that expresses epitope-tagged transgenes in Leishmania , the MHTAP tag (which bears a Myc epitope, six histidines, a protein A domain, and calmodulin binding peptide) was amplified from the plasmid pLEW-MHTAP ( ) with the primers MHTAP-BamHI-S and MHTAP-Not1-AS (all primers use in this study are described in Supplementary table S10). .. Following cleavage with BamHI and NotI, the PCR fragment was inserted into BglII+Not1-digested pLEXSY-I-bleCherry3 (Jena Biosciences).

    Clone Assay:

    Article Title: High protection of mice against Brucella abortus by oral immunization with recombinant probiotic Lactobacillus casei vector vaccine, expressing the outer membrane protein OMP19 of Brucella species.
    Article Snippet: Brucellosis is a zoonotic disease threatening the public health and hindering the trade of animals and their products, which has a negative impact on the economic development of a country. .. Brucellosis is a zoonotic disease threatening the public health and hindering the trade of animals and their products, which has a negative impact on the economic development of a country. .. Brucellosis is a zoonotic disease threatening the public health and hindering the trade of animals and their products, which has a negative impact on the economic development of a country.

    Expressing:

    Article Title: High protection of mice against Brucella abortus by oral immunization with recombinant probiotic Lactobacillus casei vector vaccine, expressing the outer membrane protein OMP19 of Brucella species.
    Article Snippet: Brucellosis is a zoonotic disease threatening the public health and hindering the trade of animals and their products, which has a negative impact on the economic development of a country. .. Brucellosis is a zoonotic disease threatening the public health and hindering the trade of animals and their products, which has a negative impact on the economic development of a country. .. Brucellosis is a zoonotic disease threatening the public health and hindering the trade of animals and their products, which has a negative impact on the economic development of a country.

    Article Title: Fatty acid and retinol-binding protein: A novel antigen for immunodiagnosis of human strongyloidiasis
    Article Snippet: White colonies harboring the target construct was selected for enzymatic digestion. .. The vector was digested with BamHI and XhoI (Jena Bioscience, Jena, Germany) and the insert was ligated into the pET28a(+) expression vector (Novagen, USA), and was used for transformation of E . coli DH5α. .. The desired plasmid was purified by a commercial plasmid purification kit (Yekta Tajhiz Azma, Tehran, Iran) and transformed into E . coli BL21(DE3) for optimal expression of recombinant sSFAR (rSsFAR).

    Article Title: Chromatin-associated protein complexes link DNA base J and transcription termination in Leishmania
    Article Snippet: The availability of modern genome-wide approaches will no doubt provide the appropriate tools to answer these questions. .. Plasmid construction To create an expression vector that expresses epitope-tagged transgenes in Leishmania , the MHTAP tag (which bears a Myc epitope, six histidines, a protein A domain, and calmodulin binding peptide) was amplified from the plasmid pLEW-MHTAP ( ) with the primers MHTAP-BamHI-S and MHTAP-Not1-AS (all primers use in this study are described in Supplementary table S10). .. Following cleavage with BamHI and NotI, the PCR fragment was inserted into BglII+Not1-digested pLEXSY-I-bleCherry3 (Jena Biosciences).

    Article Title: Orally administered recombinant Lactobacillus casei vector vaccine expressing β-toxoid of Clostridium perfringens that induced protective immunity responses.
    Article Snippet: Clostridium perfringens types B and C cause enteritis and enterotoxemia in animals. .. Clostridium perfringens types B and C cause enteritis and enterotoxemia in animals. .. Clostridium perfringens types B and C cause enteritis and enterotoxemia in animals.

    Transformation Assay:

    Article Title: Fatty acid and retinol-binding protein: A novel antigen for immunodiagnosis of human strongyloidiasis
    Article Snippet: White colonies harboring the target construct was selected for enzymatic digestion. .. The vector was digested with BamHI and XhoI (Jena Bioscience, Jena, Germany) and the insert was ligated into the pET28a(+) expression vector (Novagen, USA), and was used for transformation of E . coli DH5α. .. The desired plasmid was purified by a commercial plasmid purification kit (Yekta Tajhiz Azma, Tehran, Iran) and transformed into E . coli BL21(DE3) for optimal expression of recombinant sSFAR (rSsFAR).

    Binding Assay:

    Article Title: Chromatin-associated protein complexes link DNA base J and transcription termination in Leishmania
    Article Snippet: The availability of modern genome-wide approaches will no doubt provide the appropriate tools to answer these questions. .. Plasmid construction To create an expression vector that expresses epitope-tagged transgenes in Leishmania , the MHTAP tag (which bears a Myc epitope, six histidines, a protein A domain, and calmodulin binding peptide) was amplified from the plasmid pLEW-MHTAP ( ) with the primers MHTAP-BamHI-S and MHTAP-Not1-AS (all primers use in this study are described in Supplementary table S10). .. Following cleavage with BamHI and NotI, the PCR fragment was inserted into BglII+Not1-digested pLEXSY-I-bleCherry3 (Jena Biosciences).

    Construct:

    Article Title: Orally administered recombinant Lactobacillus casei vector vaccine expressing β-toxoid of Clostridium perfringens that induced protective immunity responses.
    Article Snippet: Clostridium perfringens types B and C cause enteritis and enterotoxemia in animals. .. Clostridium perfringens types B and C cause enteritis and enterotoxemia in animals. .. Clostridium perfringens types B and C cause enteritis and enterotoxemia in animals.

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  • 99
    Jena Bioscience restriction endonucleases bamhi
    Agarose gel electrophoresis analysis of recombinant pET28a/ pilQ 1138-2118 with restriction enzyme digestion. Lane M; DNA marker (1 kb ), Lane 1; mono-digestion of the pET28a/ pilQ 1138-2118 vector with <t>BamHI.</t> One expected fragment was observed on the gel (∼6350 bp band). Lane 2 and 3; <t>BamHI/HindIII</t> double digested the recombinant vector with BamHI and HindIII buffer, respectively. Two expected fragments from double digestion were observed on the gel (∼5369 and 981 bp bands). Lane 4; the optimized PCR product of the pilQ 1138-2118 gene (∼ 961 bp band).
    Restriction Endonucleases Bamhi, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction endonucleases bamhi/product/Jena Bioscience
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    restriction endonucleases bamhi - by Bioz Stars, 2021-06
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    86
    Jena Bioscience bamhi restriction enzymes
    Agarose gel electrophoresis analysis of recombinant pET28a/ pilQ 1138-2118 with restriction enzyme digestion. Lane M; DNA marker (1 kb ), Lane 1; mono-digestion of the pET28a/ pilQ 1138-2118 vector with <t>BamHI.</t> One expected fragment was observed on the gel (∼6350 bp band). Lane 2 and 3; <t>BamHI/HindIII</t> double digested the recombinant vector with BamHI and HindIII buffer, respectively. Two expected fragments from double digestion were observed on the gel (∼5369 and 981 bp bands). Lane 4; the optimized PCR product of the pilQ 1138-2118 gene (∼ 961 bp band).
    Bamhi Restriction Enzymes, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamhi restriction enzymes/product/Jena Bioscience
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bamhi restriction enzymes - by Bioz Stars, 2021-06
    86/100 stars
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    Image Search Results


    Agarose gel electrophoresis analysis of recombinant pET28a/ pilQ 1138-2118 with restriction enzyme digestion. Lane M; DNA marker (1 kb ), Lane 1; mono-digestion of the pET28a/ pilQ 1138-2118 vector with BamHI. One expected fragment was observed on the gel (∼6350 bp band). Lane 2 and 3; BamHI/HindIII double digested the recombinant vector with BamHI and HindIII buffer, respectively. Two expected fragments from double digestion were observed on the gel (∼5369 and 981 bp bands). Lane 4; the optimized PCR product of the pilQ 1138-2118 gene (∼ 961 bp band).

    Journal: Avicenna Journal of Medical Biotechnology

    Article Title: Molecular characterization and Functional Analysis of the PilQ380-706: a Novel Secretin Domain in Pseudomonas aeruginosa

    doi:

    Figure Lengend Snippet: Agarose gel electrophoresis analysis of recombinant pET28a/ pilQ 1138-2118 with restriction enzyme digestion. Lane M; DNA marker (1 kb ), Lane 1; mono-digestion of the pET28a/ pilQ 1138-2118 vector with BamHI. One expected fragment was observed on the gel (∼6350 bp band). Lane 2 and 3; BamHI/HindIII double digested the recombinant vector with BamHI and HindIII buffer, respectively. Two expected fragments from double digestion were observed on the gel (∼5369 and 981 bp bands). Lane 4; the optimized PCR product of the pilQ 1138-2118 gene (∼ 961 bp band).

    Article Snippet: The vector was treated with the restriction endonucleases BamHI and HindIII (Jena Bioscience Kit, Germany) according to manufacture instruction.

    Techniques: Agarose Gel Electrophoresis, Recombinant, Marker, Plasmid Preparation, Polymerase Chain Reaction

    Agarose gel electrophoresis analysis of recombinant pET28a/ pilQ 1138-2118 with restriction enzyme digestion. Lane M; DNA marker (1 kb ), Lane 1; mono-digestion of the pET28a/ pilQ 1138-2118 vector with BamHI. One expected fragment was observed on the gel (∼6350 bp band). Lane 2 and 3; BamHI/HindIII double digested the recombinant vector with BamHI and HindIII buffer, respectively. Two expected fragments from double digestion were observed on the gel (∼5369 and 981 bp bands). Lane 4; the optimized PCR product of the pilQ 1138-2118 gene (∼ 961 bp band).

    Journal: Avicenna Journal of Medical Biotechnology

    Article Title: Molecular characterization and Functional Analysis of the PilQ380-706: a Novel Secretin Domain in Pseudomonas aeruginosa

    doi:

    Figure Lengend Snippet: Agarose gel electrophoresis analysis of recombinant pET28a/ pilQ 1138-2118 with restriction enzyme digestion. Lane M; DNA marker (1 kb ), Lane 1; mono-digestion of the pET28a/ pilQ 1138-2118 vector with BamHI. One expected fragment was observed on the gel (∼6350 bp band). Lane 2 and 3; BamHI/HindIII double digested the recombinant vector with BamHI and HindIII buffer, respectively. Two expected fragments from double digestion were observed on the gel (∼5369 and 981 bp bands). Lane 4; the optimized PCR product of the pilQ 1138-2118 gene (∼ 961 bp band).

    Article Snippet: The vector was treated with the restriction endonucleases BamHI and HindIII (Jena Bioscience Kit, Germany) according to manufacture instruction.

    Techniques: Agarose Gel Electrophoresis, Recombinant, Marker, Plasmid Preparation, Polymerase Chain Reaction

    Agarose gel electrophoresis analysis of recombinant pET28a/ fliC with restriction enzyme digestion. Lane M; DNA molecular weight marker (1 kb); lane 1: mono-digestion of the pET28a/ fliC vector with BamHI. One expected fragment was observed on the gel (~ 6836 bp band). Lane 2: BamHI/HindIII digested the recombinant vector. Two expected fragments from double digestion were observed on the gel (~ 5369 and 1467 bp bands). Lane 3; the optimized PCR product of the fliC gene (~ 1467 bp band

    Journal: International Journal of Molecular and Cellular Medicine

    Article Title: High Yield Overexpression, Refolding, Purification and Characterization of Pseudomonas aeruginosa Type B-Flagellin: An Improved Method Without Sonication

    doi:

    Figure Lengend Snippet: Agarose gel electrophoresis analysis of recombinant pET28a/ fliC with restriction enzyme digestion. Lane M; DNA molecular weight marker (1 kb); lane 1: mono-digestion of the pET28a/ fliC vector with BamHI. One expected fragment was observed on the gel (~ 6836 bp band). Lane 2: BamHI/HindIII digested the recombinant vector. Two expected fragments from double digestion were observed on the gel (~ 5369 and 1467 bp bands). Lane 3; the optimized PCR product of the fliC gene (~ 1467 bp band

    Article Snippet: The recombinant vector was also treated with the restriction endonucleases BamHI and HindIII (Jena Bioscience Kit, Germany) according to manufacturer's instruction.

    Techniques: Agarose Gel Electrophoresis, Recombinant, Molecular Weight, Marker, Plasmid Preparation, Polymerase Chain Reaction