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Addgene inc bamhi restriction sites
Bamhi Restriction Sites, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bamhi restriction sites/product/Addgene inc
Average 91 stars, based on 6 article reviews
Price from $9.99 to $1999.99
bamhi restriction sites - by Bioz Stars, 2020-07
91/100 stars

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Clone Assay:

Article Title: The PARK10 gene USP24 is a negative regulator of autophagy and ULK1 protein stability
Article Snippet: .. H4-mCherry-GFP-LC3 dual reporter cells were constructed by transducing H4 cells with a mCherry-GFP-LC3 (Addgene, plasmid 22418; Jayanta Debnath Lab) construct cloned into the lentiviral pLESIP vector with NheI and BamHI restriction sites (available through Addgene, plasmid 104941). ..

Article Title: GEMC1 is a critical regulator of multiciliated cell differentiation
Article Snippet: .. E2F4 and DP1 cDNAs (Addgene plasmids #10914 and #37968) were cloned into the mammalian expression vector pcDNA3.1‐HA (Addgene) at the EcoRI and BamHI restriction sites to produce proteins N‐terminally fused to HA under the control of the constitutive CMV promoter. pCMV‐HA‐E2F5 and pCMV‐HA‐E2F1 were a gift from Kristian Helin (Addgene plasmids #24213 and #24225). .. Human Geminin cDNA (OriGene SC319331) was cloned into pcDNA5FRT/TO (Invitrogen) vector, modified with an N‐terminal YFP‐tag.

Article Title: Role of Dopamine Receptors in the Anticancer Activity of ONC201
Article Snippet: .. PCR primers contained EcoRV and BamHI restriction sites to facilitate cloning into plasmid pLCV2 (Addgene #52961). ..

Amplification:

Article Title: Affimer proteins for F-actin: novel affinity reagents that label F-actin in live and fixed cells
Article Snippet: .. Sequences lacking the His-tag and cysteine residue were amplified and the resulting cDNA inserted into the EcoRI and BamHI restriction sites, downstream of and in frame with the eGFP coding sequence. mEGFP-Lifeact-7 was a gift from Michael Davidson (Addgene plasmid # 54610, derived from a 17 amino acid residue actin-binding peptide fused to the C-terminus of eGFP). .. Td-tomato F-tractin was a gift from Wolfgang Wagner (NIH) and consists of Td-tomato fused to the N-terminus of a 43 amino acid actin binding peptide derived from inositol 1,4,5 Trisphosphate 3-kinase A ).

Synthesized:

Article Title: Fast single-cell biochemistry: theory, open source microscopy and applications
Article Snippet: .. MitAteam was generated by inserting two copies of the cytochrome c oxidase subunit VIII mitochondrial targeting sequence in tandem (synthesized with the GeneArt String DNA fragment service by ThermoFisher) between the HindIII and BamHI restriction sites of the original ATeam plamids, a gift from Takeharu Nagai (AddGene, ##51958 [ ]). .. Binding of ATP induces a conformational change that result in differences in FRET efficiencies.

Construct:

Article Title: The PARK10 gene USP24 is a negative regulator of autophagy and ULK1 protein stability
Article Snippet: .. H4-mCherry-GFP-LC3 dual reporter cells were constructed by transducing H4 cells with a mCherry-GFP-LC3 (Addgene, plasmid 22418; Jayanta Debnath Lab) construct cloned into the lentiviral pLESIP vector with NheI and BamHI restriction sites (available through Addgene, plasmid 104941). ..

Sequencing:

Article Title: Activated CaMKIIα Binds to the mGlu5 Metabotropic Glutamate Receptor and Modulates Calcium Mobilization
Article Snippet: .. The pCGN plasmid to express wild-type (WT) mGlu5a with an N-terminal hemagglutinin (HA) tag was made by amplifying the entire rat mGlu5a coding sequence (forward primer: 5′-TGACGTGCCTGACTATGCCTCTAGAATGGTCCTTCTGTTGATCCT-3′; reverse primer: 5′-ACTCACCCTGAAGTTCTCAGGATCCTCACAACGATGAAGAACTCT-3′) and inserting the fragment into XbaI and BamHI restriction sites of the empty pCGN plasmid [a gift from Dr. Winship Herr, Université de Lausanne, Switzerland; Addgene (Cambridge, MA) plasmid ID 53308]. .. The K866 RR868 mutation to AAA in mGlu5a was generated by site-directed mutagenesis of the pGEX6P or pCGN constructs (see above) using a Quick Change protocol (Agilent Technologies, Santa Clara, CA) with the following primers: forward, 5′-GGGTTTCCCCAGAGGAGC CGGCGGCGG CCCACAGGTTGACTAGGCTGCT-3′; and reverse, 5′-AGCAGCCTAGTCAACCTGTGGG CCGCCGCCGG CTCCTCTGGGGAAACCC-3′.

Article Title: Affimer proteins for F-actin: novel affinity reagents that label F-actin in live and fixed cells
Article Snippet: .. Sequences lacking the His-tag and cysteine residue were amplified and the resulting cDNA inserted into the EcoRI and BamHI restriction sites, downstream of and in frame with the eGFP coding sequence. mEGFP-Lifeact-7 was a gift from Michael Davidson (Addgene plasmid # 54610, derived from a 17 amino acid residue actin-binding peptide fused to the C-terminus of eGFP). .. Td-tomato F-tractin was a gift from Wolfgang Wagner (NIH) and consists of Td-tomato fused to the N-terminus of a 43 amino acid actin binding peptide derived from inositol 1,4,5 Trisphosphate 3-kinase A ).

Article Title: Activated CaMKIIα Binds to the mGlu5 Metabotropic Glutamate Receptor and Modulates Calcium Mobilization
Article Snippet: .. The pCGN plasmid to express wild-type (WT) mGlu5a with an N-terminal hemagglutinin (HA) tag was made by amplifying the entire rat mGlu5a coding sequence (forward primer: 5′-TGACGTGCCTGACTATGCCTCTAGAATGGTCCTTCTGTTGATCCT-3′; reverse primer: 5′-ACTCACCCTGAAGTTCTCAGGATCCTCACAACGATGAAGAACTCT-3′) and inserting the fragment into XbaI and BamHI restriction sites of the empty pCGN plasmid [a gift from Dr. Winship Herr, Université de Lausanne, Switzerland; Addgene (Cambridge, MA) plasmid ID 53308]. .. The K866 RR868 mutation to AAA in mGlu5a was generated by site-directed mutagenesis of the pGEX6P or pCGN constructs (see above) using a Quick Change protocol (Agilent Technologies, Santa Clara, CA) with the following primers: forward, 5′-GGGTTTCCCCAGAGGAGC CGGCGGCGG CCCACAGGTTGACTAGGCTGCT-3′; and reverse, 5′-AGCAGCCTAGTCAACCTGTGGG CCGCCGCCGG CTCCTCTGGGGAAACCC-3′.

Article Title: Fast single-cell biochemistry: theory, open source microscopy and applications
Article Snippet: .. MitAteam was generated by inserting two copies of the cytochrome c oxidase subunit VIII mitochondrial targeting sequence in tandem (synthesized with the GeneArt String DNA fragment service by ThermoFisher) between the HindIII and BamHI restriction sites of the original ATeam plamids, a gift from Takeharu Nagai (AddGene, ##51958 [ ]). .. Binding of ATP induces a conformational change that result in differences in FRET efficiencies.

Generated:

Article Title: Fast single-cell biochemistry: theory, open source microscopy and applications
Article Snippet: .. MitAteam was generated by inserting two copies of the cytochrome c oxidase subunit VIII mitochondrial targeting sequence in tandem (synthesized with the GeneArt String DNA fragment service by ThermoFisher) between the HindIII and BamHI restriction sites of the original ATeam plamids, a gift from Takeharu Nagai (AddGene, ##51958 [ ]). .. Binding of ATP induces a conformational change that result in differences in FRET efficiencies.

Plasmid Preparation:

Article Title: Activated CaMKIIα Binds to the mGlu5 Metabotropic Glutamate Receptor and Modulates Calcium Mobilization
Article Snippet: .. The pCGN plasmid to express wild-type (WT) mGlu5a with an N-terminal hemagglutinin (HA) tag was made by amplifying the entire rat mGlu5a coding sequence (forward primer: 5′-TGACGTGCCTGACTATGCCTCTAGAATGGTCCTTCTGTTGATCCT-3′; reverse primer: 5′-ACTCACCCTGAAGTTCTCAGGATCCTCACAACGATGAAGAACTCT-3′) and inserting the fragment into XbaI and BamHI restriction sites of the empty pCGN plasmid [a gift from Dr. Winship Herr, Université de Lausanne, Switzerland; Addgene (Cambridge, MA) plasmid ID 53308]. .. The K866 RR868 mutation to AAA in mGlu5a was generated by site-directed mutagenesis of the pGEX6P or pCGN constructs (see above) using a Quick Change protocol (Agilent Technologies, Santa Clara, CA) with the following primers: forward, 5′-GGGTTTCCCCAGAGGAGC CGGCGGCGG CCCACAGGTTGACTAGGCTGCT-3′; and reverse, 5′-AGCAGCCTAGTCAACCTGTGGG CCGCCGCCGG CTCCTCTGGGGAAACCC-3′.

Article Title: The PARK10 gene USP24 is a negative regulator of autophagy and ULK1 protein stability
Article Snippet: .. H4-mCherry-GFP-LC3 dual reporter cells were constructed by transducing H4 cells with a mCherry-GFP-LC3 (Addgene, plasmid 22418; Jayanta Debnath Lab) construct cloned into the lentiviral pLESIP vector with NheI and BamHI restriction sites (available through Addgene, plasmid 104941). ..

Article Title: GEMC1 is a critical regulator of multiciliated cell differentiation
Article Snippet: .. E2F4 and DP1 cDNAs (Addgene plasmids #10914 and #37968) were cloned into the mammalian expression vector pcDNA3.1‐HA (Addgene) at the EcoRI and BamHI restriction sites to produce proteins N‐terminally fused to HA under the control of the constitutive CMV promoter. pCMV‐HA‐E2F5 and pCMV‐HA‐E2F1 were a gift from Kristian Helin (Addgene plasmids #24213 and #24225). .. Human Geminin cDNA (OriGene SC319331) was cloned into pcDNA5FRT/TO (Invitrogen) vector, modified with an N‐terminal YFP‐tag.

Article Title: Role of Dopamine Receptors in the Anticancer Activity of ONC201
Article Snippet: .. PCR primers contained EcoRV and BamHI restriction sites to facilitate cloning into plasmid pLCV2 (Addgene #52961). ..

Article Title: Affimer proteins for F-actin: novel affinity reagents that label F-actin in live and fixed cells
Article Snippet: .. Sequences lacking the His-tag and cysteine residue were amplified and the resulting cDNA inserted into the EcoRI and BamHI restriction sites, downstream of and in frame with the eGFP coding sequence. mEGFP-Lifeact-7 was a gift from Michael Davidson (Addgene plasmid # 54610, derived from a 17 amino acid residue actin-binding peptide fused to the C-terminus of eGFP). .. Td-tomato F-tractin was a gift from Wolfgang Wagner (NIH) and consists of Td-tomato fused to the N-terminus of a 43 amino acid actin binding peptide derived from inositol 1,4,5 Trisphosphate 3-kinase A ).

Article Title: Activated CaMKIIα Binds to the mGlu5 Metabotropic Glutamate Receptor and Modulates Calcium Mobilization
Article Snippet: .. The pCGN plasmid to express wild-type (WT) mGlu5a with an N-terminal hemagglutinin (HA) tag was made by amplifying the entire rat mGlu5a coding sequence (forward primer: 5′-TGACGTGCCTGACTATGCCTCTAGAATGGTCCTTCTGTTGATCCT-3′; reverse primer: 5′-ACTCACCCTGAAGTTCTCAGGATCCTCACAACGATGAAGAACTCT-3′) and inserting the fragment into XbaI and BamHI restriction sites of the empty pCGN plasmid [a gift from Dr. Winship Herr, Université de Lausanne, Switzerland; Addgene (Cambridge, MA) plasmid ID 53308]. .. The K866 RR868 mutation to AAA in mGlu5a was generated by site-directed mutagenesis of the pGEX6P or pCGN constructs (see above) using a Quick Change protocol (Agilent Technologies, Santa Clara, CA) with the following primers: forward, 5′-GGGTTTCCCCAGAGGAGC CGGCGGCGG CCCACAGGTTGACTAGGCTGCT-3′; and reverse, 5′-AGCAGCCTAGTCAACCTGTGGG CCGCCGCCGG CTCCTCTGGGGAAACCC-3′.

Expressing:

Article Title: GEMC1 is a critical regulator of multiciliated cell differentiation
Article Snippet: .. E2F4 and DP1 cDNAs (Addgene plasmids #10914 and #37968) were cloned into the mammalian expression vector pcDNA3.1‐HA (Addgene) at the EcoRI and BamHI restriction sites to produce proteins N‐terminally fused to HA under the control of the constitutive CMV promoter. pCMV‐HA‐E2F5 and pCMV‐HA‐E2F1 were a gift from Kristian Helin (Addgene plasmids #24213 and #24225). .. Human Geminin cDNA (OriGene SC319331) was cloned into pcDNA5FRT/TO (Invitrogen) vector, modified with an N‐terminal YFP‐tag.

Polymerase Chain Reaction:

Article Title: Role of Dopamine Receptors in the Anticancer Activity of ONC201
Article Snippet: .. PCR primers contained EcoRV and BamHI restriction sites to facilitate cloning into plasmid pLCV2 (Addgene #52961). ..

Derivative Assay:

Article Title: Affimer proteins for F-actin: novel affinity reagents that label F-actin in live and fixed cells
Article Snippet: .. Sequences lacking the His-tag and cysteine residue were amplified and the resulting cDNA inserted into the EcoRI and BamHI restriction sites, downstream of and in frame with the eGFP coding sequence. mEGFP-Lifeact-7 was a gift from Michael Davidson (Addgene plasmid # 54610, derived from a 17 amino acid residue actin-binding peptide fused to the C-terminus of eGFP). .. Td-tomato F-tractin was a gift from Wolfgang Wagner (NIH) and consists of Td-tomato fused to the N-terminus of a 43 amino acid actin binding peptide derived from inositol 1,4,5 Trisphosphate 3-kinase A ).

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  • 91
    Addgene inc full length human hdac3
    Positional effects of <t>dCas9-HDAC3</t> on gene expression at three different loci. ( a ) RNA-seq and H3K27ac ChIP-seq reads mapped to a region encompassing the murine Smn1 gene. Magnified insets of H3K27ac enrichment at the Smn1 promoter locus with approximate locations of each gRNA-targeted site are displayed below. ( b ) Quantitative RT–PCR measurements of relative mRNA levels of Smn1 from N2a cells co-transfected with each gRNA and the indicated dCas9 proteins (relative to cells transfected with dCas9-HDAC3 alone; red dashed line) * P
    Full Length Human Hdac3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/full length human hdac3/product/Addgene inc
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    93
    Addgene inc bamhi noti restriction sites
    Positional effects of <t>dCas9-HDAC3</t> on gene expression at three different loci. ( a ) RNA-seq and H3K27ac ChIP-seq reads mapped to a region encompassing the murine Smn1 gene. Magnified insets of H3K27ac enrichment at the Smn1 promoter locus with approximate locations of each gRNA-targeted site are displayed below. ( b ) Quantitative RT–PCR measurements of relative mRNA levels of Smn1 from N2a cells co-transfected with each gRNA and the indicated dCas9 proteins (relative to cells transfected with dCas9-HDAC3 alone; red dashed line) * P
    Bamhi Noti Restriction Sites, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamhi noti restriction sites/product/Addgene inc
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
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    92
    Addgene inc bamhi paci restriction sites
    Positional effects of <t>dCas9-HDAC3</t> on gene expression at three different loci. ( a ) RNA-seq and H3K27ac ChIP-seq reads mapped to a region encompassing the murine Smn1 gene. Magnified insets of H3K27ac enrichment at the Smn1 promoter locus with approximate locations of each gRNA-targeted site are displayed below. ( b ) Quantitative RT–PCR measurements of relative mRNA levels of Smn1 from N2a cells co-transfected with each gRNA and the indicated dCas9 proteins (relative to cells transfected with dCas9-HDAC3 alone; red dashed line) * P
    Bamhi Paci Restriction Sites, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamhi paci restriction sites/product/Addgene inc
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bamhi paci restriction sites - by Bioz Stars, 2020-07
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      Buy from Supplier

    88
    Addgene inc ecori bamhi restriction sites
    Positional effects of <t>dCas9-HDAC3</t> on gene expression at three different loci. ( a ) RNA-seq and H3K27ac ChIP-seq reads mapped to a region encompassing the murine Smn1 gene. Magnified insets of H3K27ac enrichment at the Smn1 promoter locus with approximate locations of each gRNA-targeted site are displayed below. ( b ) Quantitative RT–PCR measurements of relative mRNA levels of Smn1 from N2a cells co-transfected with each gRNA and the indicated dCas9 proteins (relative to cells transfected with dCas9-HDAC3 alone; red dashed line) * P
    Ecori Bamhi Restriction Sites, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecori bamhi restriction sites/product/Addgene inc
    Average 88 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
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    Positional effects of dCas9-HDAC3 on gene expression at three different loci. ( a ) RNA-seq and H3K27ac ChIP-seq reads mapped to a region encompassing the murine Smn1 gene. Magnified insets of H3K27ac enrichment at the Smn1 promoter locus with approximate locations of each gRNA-targeted site are displayed below. ( b ) Quantitative RT–PCR measurements of relative mRNA levels of Smn1 from N2a cells co-transfected with each gRNA and the indicated dCas9 proteins (relative to cells transfected with dCas9-HDAC3 alone; red dashed line) * P

    Journal: Nature Communications

    Article Title: Locus-specific histone deacetylation using a synthetic CRISPR-Cas9-based HDAC

    doi: 10.1038/ncomms15315

    Figure Lengend Snippet: Positional effects of dCas9-HDAC3 on gene expression at three different loci. ( a ) RNA-seq and H3K27ac ChIP-seq reads mapped to a region encompassing the murine Smn1 gene. Magnified insets of H3K27ac enrichment at the Smn1 promoter locus with approximate locations of each gRNA-targeted site are displayed below. ( b ) Quantitative RT–PCR measurements of relative mRNA levels of Smn1 from N2a cells co-transfected with each gRNA and the indicated dCas9 proteins (relative to cells transfected with dCas9-HDAC3 alone; red dashed line) * P

    Article Snippet: The VP64 effector domain was removed from the plasmid by digestion with BamHI and BsrGI and full-length human HDAC3 (PCR amplified from Addgene plasmid #13819 with BamHI/BsrGI restriction sites) was cloned into the pLenti-dCas9VP64 backbone.

    Techniques: Expressing, RNA Sequencing Assay, Chromatin Immunoprecipitation, Quantitative RT-PCR, Transfection

    Design of Cas9-based HDAC system. ( a ) Schematic of dCas9 fusion constructs, dCas9-HDAC3 and dCas9-HDAC3 R265P , and gRNA construct. ( b ) RPKM values for Isl1, Mecp2 and Smn1 in murine N2a cells as determined by RNA-seq ( n =2 biological replicates; error bars, s.e.m.). ( c ) Isl1, Mecp2 and Smn1 expression, relative to Mecp2 (red dashed line) in N2a cells ( n =3 biological replicates; error bars, s.e.m.).

    Journal: Nature Communications

    Article Title: Locus-specific histone deacetylation using a synthetic CRISPR-Cas9-based HDAC

    doi: 10.1038/ncomms15315

    Figure Lengend Snippet: Design of Cas9-based HDAC system. ( a ) Schematic of dCas9 fusion constructs, dCas9-HDAC3 and dCas9-HDAC3 R265P , and gRNA construct. ( b ) RPKM values for Isl1, Mecp2 and Smn1 in murine N2a cells as determined by RNA-seq ( n =2 biological replicates; error bars, s.e.m.). ( c ) Isl1, Mecp2 and Smn1 expression, relative to Mecp2 (red dashed line) in N2a cells ( n =3 biological replicates; error bars, s.e.m.).

    Article Snippet: The VP64 effector domain was removed from the plasmid by digestion with BamHI and BsrGI and full-length human HDAC3 (PCR amplified from Addgene plasmid #13819 with BamHI/BsrGI restriction sites) was cloned into the pLenti-dCas9VP64 backbone.

    Techniques: Construct, RNA Sequencing Assay, Expressing

    Generation of dCas9-HDAC3 and dCas9-HDAC3 R265P MC3T3-e1 clonal cell lines targeting the Mecp2 promoter. ( a ) RT–PCR analysis of Mecp2 expression in MC3T3-e1 cells relative to that of N2a cells. *** P

    Journal: Nature Communications

    Article Title: Locus-specific histone deacetylation using a synthetic CRISPR-Cas9-based HDAC

    doi: 10.1038/ncomms15315

    Figure Lengend Snippet: Generation of dCas9-HDAC3 and dCas9-HDAC3 R265P MC3T3-e1 clonal cell lines targeting the Mecp2 promoter. ( a ) RT–PCR analysis of Mecp2 expression in MC3T3-e1 cells relative to that of N2a cells. *** P

    Article Snippet: The VP64 effector domain was removed from the plasmid by digestion with BamHI and BsrGI and full-length human HDAC3 (PCR amplified from Addgene plasmid #13819 with BamHI/BsrGI restriction sites) was cloned into the pLenti-dCas9VP64 backbone.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing

    Assessing gRNA dosage and dCas9-HDAC3 range of activity at the Mecp2 locus. ( a ) The Mecp2 promoter locus is schematically depicted with the approximate locations of gRNA-3 and the ChIP-qPCR amplicon regions (in blue). ( b ) H3K27ac ChIP-qPCR enrichment (relative to dCas9-HDAC3 R265P ) at regions in the Mecp2 promoter depicted in Fig. 2c . One-way ANOVA with Dunnett post-test; * P

    Journal: Nature Communications

    Article Title: Locus-specific histone deacetylation using a synthetic CRISPR-Cas9-based HDAC

    doi: 10.1038/ncomms15315

    Figure Lengend Snippet: Assessing gRNA dosage and dCas9-HDAC3 range of activity at the Mecp2 locus. ( a ) The Mecp2 promoter locus is schematically depicted with the approximate locations of gRNA-3 and the ChIP-qPCR amplicon regions (in blue). ( b ) H3K27ac ChIP-qPCR enrichment (relative to dCas9-HDAC3 R265P ) at regions in the Mecp2 promoter depicted in Fig. 2c . One-way ANOVA with Dunnett post-test; * P

    Article Snippet: The VP64 effector domain was removed from the plasmid by digestion with BamHI and BsrGI and full-length human HDAC3 (PCR amplified from Addgene plasmid #13819 with BamHI/BsrGI restriction sites) was cloned into the pLenti-dCas9VP64 backbone.

    Techniques: Activity Assay, Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction, Amplification