bamhi restriction enzymes  (Thermo Fisher)


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    Structured Review

    Thermo Fisher bamhi restriction enzymes
    Bamhi Restriction Enzymes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamhi restriction enzymes/product/Thermo Fisher
    Average 98 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    bamhi restriction enzymes - by Bioz Stars, 2020-01
    98/100 stars

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    Related Articles

    Diagnostic Assay:

    Article Title: The Effect of Bacterial Peptide p28 on Viability and Apoptosis Status of P53-null HeLa Cells
    Article Snippet: .. Expression and purification of recombinant p28 The 84 bp fragment of p28 gene was cloned into pET-28a expression plasmid vector (provided by Diagnostic Laboratory Sciences and Technology Research Center, Shiraz, Iran) using NdeI and BamHI restriction enzymes and T4 DNA ligase (Fermentas, Vilnius, Litvanya). .. The constructed vector was authenticated by enzyme digestion and Sanger sequencing.

    Clone Assay:

    Article Title: The Effect of Bacterial Peptide p28 on Viability and Apoptosis Status of P53-null HeLa Cells
    Article Snippet: .. Expression and purification of recombinant p28 The 84 bp fragment of p28 gene was cloned into pET-28a expression plasmid vector (provided by Diagnostic Laboratory Sciences and Technology Research Center, Shiraz, Iran) using NdeI and BamHI restriction enzymes and T4 DNA ligase (Fermentas, Vilnius, Litvanya). .. The constructed vector was authenticated by enzyme digestion and Sanger sequencing.

    Positron Emission Tomography:

    Article Title: The Effect of Bacterial Peptide p28 on Viability and Apoptosis Status of P53-null HeLa Cells
    Article Snippet: .. Expression and purification of recombinant p28 The 84 bp fragment of p28 gene was cloned into pET-28a expression plasmid vector (provided by Diagnostic Laboratory Sciences and Technology Research Center, Shiraz, Iran) using NdeI and BamHI restriction enzymes and T4 DNA ligase (Fermentas, Vilnius, Litvanya). .. The constructed vector was authenticated by enzyme digestion and Sanger sequencing.

    Isolation:

    Article Title: The Effect of Bacterial Peptide p28 on Viability and Apoptosis Status of P53-null HeLa Cells
    Article Snippet: .. Polymerase Chain Reaction Amplification of p28 Gene Total DNA was isolated from P. aeruginosa bacterial strain (gifted from Department of Medical Laboratory Sciences of Shiraz University of Medical Sciences (SUMS), Shiraz, Iran) using boiling method as described by other studies , and used as a template for polymerase chain reaction ( PCR) amplification of p28 gene. p28-Forward and -Reverse primers ( ) with appropriate restriction sites for NdeI and BamHI restriction enzymes (Thermo Scientific, Massachusetts, USA)‎ were designed using Allele Id version 7.5 (Premier Biosoft, California, USA) and ordered for synthesis (Metabion, Steinkirchen, Germany). .. PCR was carried out in a 20 µL reaction mixture containing Hot-start Taq polymerase (Ampliqon, Odense, Denmark).PCR cycles consisted of15 minutes of initial denaturation at 95°C, 30 seconds of denaturation at 95°C, 30 seconds of annealing at 58°C, and 30 seconds of elongation at 72°C in addition to a final extension for 5 minutes at 72°C.

    Construct:

    Article Title: The Effect of Bacterial Peptide p28 on Viability and Apoptosis Status of P53-null HeLa Cells
    Article Snippet: Expression and purification of recombinant p28 The 84 bp fragment of p28 gene was cloned into pET-28a expression plasmid vector (provided by Diagnostic Laboratory Sciences and Technology Research Center, Shiraz, Iran) using NdeI and BamHI restriction enzymes and T4 DNA ligase (Fermentas, Vilnius, Litvanya). .. The constructed vector was authenticated by enzyme digestion and Sanger sequencing.

    Purification:

    Article Title: The Effect of Bacterial Peptide p28 on Viability and Apoptosis Status of P53-null HeLa Cells
    Article Snippet: .. Expression and purification of recombinant p28 The 84 bp fragment of p28 gene was cloned into pET-28a expression plasmid vector (provided by Diagnostic Laboratory Sciences and Technology Research Center, Shiraz, Iran) using NdeI and BamHI restriction enzymes and T4 DNA ligase (Fermentas, Vilnius, Litvanya). .. The constructed vector was authenticated by enzyme digestion and Sanger sequencing.

    Sequencing:

    Article Title: The Effect of Bacterial Peptide p28 on Viability and Apoptosis Status of P53-null HeLa Cells
    Article Snippet: Expression and purification of recombinant p28 The 84 bp fragment of p28 gene was cloned into pET-28a expression plasmid vector (provided by Diagnostic Laboratory Sciences and Technology Research Center, Shiraz, Iran) using NdeI and BamHI restriction enzymes and T4 DNA ligase (Fermentas, Vilnius, Litvanya). .. The constructed vector was authenticated by enzyme digestion and Sanger sequencing.

    Incubation:

    Article Title: The Effect of Bacterial Peptide p28 on Viability and Apoptosis Status of P53-null HeLa Cells
    Article Snippet: Expression and purification of recombinant p28 The 84 bp fragment of p28 gene was cloned into pET-28a expression plasmid vector (provided by Diagnostic Laboratory Sciences and Technology Research Center, Shiraz, Iran) using NdeI and BamHI restriction enzymes and T4 DNA ligase (Fermentas, Vilnius, Litvanya). .. A single colony of the transformed bacteria was inoculated into 50 mL of LB broth (70 µg/mL of kanamycin), incubated at 37°C until the OD 600 absorbance reached 0.5–0.6.

    Amplification:

    Article Title: The Effect of Bacterial Peptide p28 on Viability and Apoptosis Status of P53-null HeLa Cells
    Article Snippet: .. Polymerase Chain Reaction Amplification of p28 Gene Total DNA was isolated from P. aeruginosa bacterial strain (gifted from Department of Medical Laboratory Sciences of Shiraz University of Medical Sciences (SUMS), Shiraz, Iran) using boiling method as described by other studies , and used as a template for polymerase chain reaction ( PCR) amplification of p28 gene. p28-Forward and -Reverse primers ( ) with appropriate restriction sites for NdeI and BamHI restriction enzymes (Thermo Scientific, Massachusetts, USA)‎ were designed using Allele Id version 7.5 (Premier Biosoft, California, USA) and ordered for synthesis (Metabion, Steinkirchen, Germany). .. PCR was carried out in a 20 µL reaction mixture containing Hot-start Taq polymerase (Ampliqon, Odense, Denmark).PCR cycles consisted of15 minutes of initial denaturation at 95°C, 30 seconds of denaturation at 95°C, 30 seconds of annealing at 58°C, and 30 seconds of elongation at 72°C in addition to a final extension for 5 minutes at 72°C.

    Cell Culture:

    Article Title: The Effect of Bacterial Peptide p28 on Viability and Apoptosis Status of P53-null HeLa Cells
    Article Snippet: Expression and purification of recombinant p28 The 84 bp fragment of p28 gene was cloned into pET-28a expression plasmid vector (provided by Diagnostic Laboratory Sciences and Technology Research Center, Shiraz, Iran) using NdeI and BamHI restriction enzymes and T4 DNA ligase (Fermentas, Vilnius, Litvanya). .. The plasmid vector was transformed into E. coli BL21 (DE3) bacteria (gifted from Department of Medical Laboratory Sciences of SUMS, Shiraz, Iran) and cultured on agar plate containing kanamycin (70 µg/mL).

    Expressing:

    Article Title: The Effect of Bacterial Peptide p28 on Viability and Apoptosis Status of P53-null HeLa Cells
    Article Snippet: .. Expression and purification of recombinant p28 The 84 bp fragment of p28 gene was cloned into pET-28a expression plasmid vector (provided by Diagnostic Laboratory Sciences and Technology Research Center, Shiraz, Iran) using NdeI and BamHI restriction enzymes and T4 DNA ligase (Fermentas, Vilnius, Litvanya). .. The constructed vector was authenticated by enzyme digestion and Sanger sequencing.

    Polymerase Chain Reaction:

    Article Title: The Effect of Bacterial Peptide p28 on Viability and Apoptosis Status of P53-null HeLa Cells
    Article Snippet: .. Polymerase Chain Reaction Amplification of p28 Gene Total DNA was isolated from P. aeruginosa bacterial strain (gifted from Department of Medical Laboratory Sciences of Shiraz University of Medical Sciences (SUMS), Shiraz, Iran) using boiling method as described by other studies , and used as a template for polymerase chain reaction ( PCR) amplification of p28 gene. p28-Forward and -Reverse primers ( ) with appropriate restriction sites for NdeI and BamHI restriction enzymes (Thermo Scientific, Massachusetts, USA)‎ were designed using Allele Id version 7.5 (Premier Biosoft, California, USA) and ordered for synthesis (Metabion, Steinkirchen, Germany). .. PCR was carried out in a 20 µL reaction mixture containing Hot-start Taq polymerase (Ampliqon, Odense, Denmark).PCR cycles consisted of15 minutes of initial denaturation at 95°C, 30 seconds of denaturation at 95°C, 30 seconds of annealing at 58°C, and 30 seconds of elongation at 72°C in addition to a final extension for 5 minutes at 72°C.

    Transformation Assay:

    Article Title: The Effect of Bacterial Peptide p28 on Viability and Apoptosis Status of P53-null HeLa Cells
    Article Snippet: Expression and purification of recombinant p28 The 84 bp fragment of p28 gene was cloned into pET-28a expression plasmid vector (provided by Diagnostic Laboratory Sciences and Technology Research Center, Shiraz, Iran) using NdeI and BamHI restriction enzymes and T4 DNA ligase (Fermentas, Vilnius, Litvanya). .. The plasmid vector was transformed into E. coli BL21 (DE3) bacteria (gifted from Department of Medical Laboratory Sciences of SUMS, Shiraz, Iran) and cultured on agar plate containing kanamycin (70 µg/mL).

    Recombinant:

    Article Title: The Effect of Bacterial Peptide p28 on Viability and Apoptosis Status of P53-null HeLa Cells
    Article Snippet: .. Expression and purification of recombinant p28 The 84 bp fragment of p28 gene was cloned into pET-28a expression plasmid vector (provided by Diagnostic Laboratory Sciences and Technology Research Center, Shiraz, Iran) using NdeI and BamHI restriction enzymes and T4 DNA ligase (Fermentas, Vilnius, Litvanya). .. The constructed vector was authenticated by enzyme digestion and Sanger sequencing.

    Plasmid Preparation:

    Article Title: The Effect of Bacterial Peptide p28 on Viability and Apoptosis Status of P53-null HeLa Cells
    Article Snippet: .. Expression and purification of recombinant p28 The 84 bp fragment of p28 gene was cloned into pET-28a expression plasmid vector (provided by Diagnostic Laboratory Sciences and Technology Research Center, Shiraz, Iran) using NdeI and BamHI restriction enzymes and T4 DNA ligase (Fermentas, Vilnius, Litvanya). .. The constructed vector was authenticated by enzyme digestion and Sanger sequencing.

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  • 90
    Thermo Fisher fastdigest restriction enzymes
    Fastdigest Restriction Enzymes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fastdigest restriction enzymes/product/Thermo Fisher
    Average 90 stars, based on 64 article reviews
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    Thermo Fisher bamhi restriction enzymes
    Bamhi Restriction Enzymes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamhi restriction enzymes/product/Thermo Fisher
    Average 90 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    bamhi restriction enzymes - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

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