bamhi restriction enzymes  (Thermo Fisher)


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    Name:
    BamHI 10 U µL
    Description:
    5 G ↓G A T C C 3 3 C C T A G ↑G 5 Thermo Scientific BamHI restriction enzyme recognizes G GATCC sites and cuts best at 37°C in its own unique buffer See Reaction Conditions for Restriction Enzymes for a table of enzyme activity conditions for double digestion and heat inactivation for this and other restriction enzymes Note Also available as a FastDigest enzyme for rapid DNA digestion Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes optimized to work in one of the buffers of the Five Buffer System In addition the universal Tango buffer is provided for convenience in double digestions All of the enzymes exhibit 100 activity in the recommended buffer and reaction conditions To ensure consistent performance Thermo Scientific restriction enzyme reaction buffers contain premixed BSA which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations Features• Superior quality stringent quality control and industry leading manufacturing process• Convenient color coded Five Buffer System• Includes universal Tango buffer for double digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificitiesApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern blotting• Restriction fragment length polymorphism RFLP • SNPNote For methylation sensitivity refer to product specifications
    Catalog Number:
    er0051
    Price:
    None
    Applications:
    Cloning|Restriction Enzyme Cloning
    Category:
    Proteins Enzymes Peptides
    Buy from Supplier


    Structured Review

    Thermo Fisher bamhi restriction enzymes
    Confirmation of Self-Ligated pP15A, kana R by Digestion Lane 2: digestion of pP15A, kana R vector with <t>BamHI</t> ; lane 3, xbaI; Lane 4, double digestion with BamHI-XbaI; Lane 5, undigested pP15A, kana R vector; Lanes 1 and 6, GeneRuler DNA ladder mix (Thermo Scientific, <t>Waltham,</t> MA, USA).
    5 G ↓G A T C C 3 3 C C T A G ↑G 5 Thermo Scientific BamHI restriction enzyme recognizes G GATCC sites and cuts best at 37°C in its own unique buffer See Reaction Conditions for Restriction Enzymes for a table of enzyme activity conditions for double digestion and heat inactivation for this and other restriction enzymes Note Also available as a FastDigest enzyme for rapid DNA digestion Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes optimized to work in one of the buffers of the Five Buffer System In addition the universal Tango buffer is provided for convenience in double digestions All of the enzymes exhibit 100 activity in the recommended buffer and reaction conditions To ensure consistent performance Thermo Scientific restriction enzyme reaction buffers contain premixed BSA which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations Features• Superior quality stringent quality control and industry leading manufacturing process• Convenient color coded Five Buffer System• Includes universal Tango buffer for double digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificitiesApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern blotting• Restriction fragment length polymorphism RFLP • SNPNote For methylation sensitivity refer to product specifications
    https://www.bioz.com/result/bamhi restriction enzymes/product/Thermo Fisher
    Average 99 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    bamhi restriction enzymes - by Bioz Stars, 2020-07
    99/100 stars

    Images

    1) Product Images from "Zinc Finger Nuclease: A New Approach to Overcome Beta-Lactam Antibiotic Resistance"

    Article Title: Zinc Finger Nuclease: A New Approach to Overcome Beta-Lactam Antibiotic Resistance

    Journal: Jundishapur Journal of Microbiology

    doi: 10.5812/jjm.29384

    Confirmation of Self-Ligated pP15A, kana R by Digestion Lane 2: digestion of pP15A, kana R vector with BamHI ; lane 3, xbaI; Lane 4, double digestion with BamHI-XbaI; Lane 5, undigested pP15A, kana R vector; Lanes 1 and 6, GeneRuler DNA ladder mix (Thermo Scientific, Waltham, MA, USA).
    Figure Legend Snippet: Confirmation of Self-Ligated pP15A, kana R by Digestion Lane 2: digestion of pP15A, kana R vector with BamHI ; lane 3, xbaI; Lane 4, double digestion with BamHI-XbaI; Lane 5, undigested pP15A, kana R vector; Lanes 1 and 6, GeneRuler DNA ladder mix (Thermo Scientific, Waltham, MA, USA).

    Techniques Used: Plasmid Preparation

    Related Articles

    Ligation:

    Article Title: Immobilized Cobalt Affinity Chromatography Provides a Novel, Efficient Method for Herpes Simplex Virus Type 1 Gene Vector Purification
    Article Snippet: .. The oligonucleotides (10 pM) were mixed in phosphate-buffered saline (PBS), denatured at 100°C for 10 min, and then annealed for 1 h. BamHI-digested plasmid pTZ18UgBpK− DNA was dephosphorylated, gel purified, and then ligated with the annealed HAT-encoding oligonucleotide mixture, and then the ligation mixture was transformed into Escherichia coli DH5α competent cells (Invitrogen). .. Single colonies from the transformation were cultured, and plasmid DNAs were extracted by use of a Quick plasmid kit (Qiagen, Valencia, Calif.).

    Incubation:

    Article Title: Characterization of RNA Helicase CshA and Its Role in Protecting mRNAs and Small RNAs of Staphylococcus aureus Strain Newman
    Article Snippet: .. To obtain sarA mRNA, pET14b- sarA (1 μg) ( ) was linearized with BamHI, incubated with components of the T7 Mega transcription kit (Ambion) for 2 h, and further treated with DNase I for 15 min at 37°C. .. The sarA transcript (5 μg) was dephosphorylated with 10 U of Antarctic phosphatase (NEB) and then radiolabeled with [γ-32 P]ATP at 37°C for 30 min in a 25-μl reaction volume using 4 U of T4 polynucleotide kinase (Invitrogen).

    Cell Culture:

    Article Title: Designing, Construction and Expression of a Recombinant Fusion Protein Comprising the Hepatitis E Virus ORF2 and Rotavirus NSP4 in the Baculovirus Expression System
    Article Snippet: .. The pBlue Script II containing fusion truncated ORF2-NSP4 was transformed in E.coli DH5α, cultured in LB agar containing ampicillin (50 mg/L), then plasmid was extracted and double digested by BamHI and SalI restriction enzymes (Thermo Scientific, USA). .. Simultaneously, PfastBac1 plasmid was also double digested by BamHI and Sal1.

    Purification:

    Article Title: Immobilized Cobalt Affinity Chromatography Provides a Novel, Efficient Method for Herpes Simplex Virus Type 1 Gene Vector Purification
    Article Snippet: .. The oligonucleotides (10 pM) were mixed in phosphate-buffered saline (PBS), denatured at 100°C for 10 min, and then annealed for 1 h. BamHI-digested plasmid pTZ18UgBpK− DNA was dephosphorylated, gel purified, and then ligated with the annealed HAT-encoding oligonucleotide mixture, and then the ligation mixture was transformed into Escherichia coli DH5α competent cells (Invitrogen). .. Single colonies from the transformation were cultured, and plasmid DNAs were extracted by use of a Quick plasmid kit (Qiagen, Valencia, Calif.).

    Generated:

    Article Title: Human Mre11/Human Rad50/Nbs1 and DNA Ligase III?/XRCC1 Protein Complexes Act Together in an Alternative Nonhomologous End Joining Pathway *
    Article Snippet: .. Linear DNA molecules were generated by digestion of pGADT7 (Invitrogen) with BamHI and EcoRI for the four nucleotide 5′ overhangs and of pcDNA4HisMax C (Invitrogen) with KpnI and PstI for the four nucleotide 3′ overhangs. .. Intramolecular joining of the incompatible ends was carried out in 25 m m MOPS (pH 7.0), 60 m m KCl, 0.2% Tween 20, 2 m m DTT, 4 m m MgCl2 , 2 m m MnCl2 , 0.5 m m ATP, 0.8 pmol of plasmid DNA, 10% polyethylene glycol, 0.01 pmol of DNA ligase III/XRCC1 or DNA ligase IV/XRCC4, and 0.06 pmol of hMre11 or MRN as indicated, in a volume of 10 μl.

    Article Title: Construction of a Novel DNA Vaccine Candidate Encoding an HspX-PPE44-EsxV Fusion Antigen of Mycobacterium tuberculosis
    Article Snippet: .. Then the generated HspX-PPE44-EsxV, along with vector, was digested with BamHI and XbaI restriction enzymes and ligated into pcDNA3.1 (+) (Invitrogen, USA) in BamHI and XbaI restriction sites at the 5´and 3´ ends ( ). .. This plasmid was used to transform competent E. coli , Top-10 strain.

    HAT Assay:

    Article Title: Immobilized Cobalt Affinity Chromatography Provides a Novel, Efficient Method for Herpes Simplex Virus Type 1 Gene Vector Purification
    Article Snippet: .. The oligonucleotides (10 pM) were mixed in phosphate-buffered saline (PBS), denatured at 100°C for 10 min, and then annealed for 1 h. BamHI-digested plasmid pTZ18UgBpK− DNA was dephosphorylated, gel purified, and then ligated with the annealed HAT-encoding oligonucleotide mixture, and then the ligation mixture was transformed into Escherichia coli DH5α competent cells (Invitrogen). .. Single colonies from the transformation were cultured, and plasmid DNAs were extracted by use of a Quick plasmid kit (Qiagen, Valencia, Calif.).

    Activity Assay:

    Article Title: Generation of DNA cleavage specificities of type II restriction endonucleases by reassortment of target recognition domains
    Article Snippet: .. BamHI-linearized DNA of pSEAd-7 was provided by Fermentas UAB and used for the determination of DNA cleavage specificity and the specific activity of hybrids. .. Hybrid genes were constructed on the backbone of plasmids pET-Alosup9 (provided by Fermentas UAB), pET-PpiI, and pET-TstIx (see ).

    Transformation Assay:

    Article Title: Designing, Construction and Expression of a Recombinant Fusion Protein Comprising the Hepatitis E Virus ORF2 and Rotavirus NSP4 in the Baculovirus Expression System
    Article Snippet: .. The pBlue Script II containing fusion truncated ORF2-NSP4 was transformed in E.coli DH5α, cultured in LB agar containing ampicillin (50 mg/L), then plasmid was extracted and double digested by BamHI and SalI restriction enzymes (Thermo Scientific, USA). .. Simultaneously, PfastBac1 plasmid was also double digested by BamHI and Sal1.

    Article Title: Immobilized Cobalt Affinity Chromatography Provides a Novel, Efficient Method for Herpes Simplex Virus Type 1 Gene Vector Purification
    Article Snippet: .. The oligonucleotides (10 pM) were mixed in phosphate-buffered saline (PBS), denatured at 100°C for 10 min, and then annealed for 1 h. BamHI-digested plasmid pTZ18UgBpK− DNA was dephosphorylated, gel purified, and then ligated with the annealed HAT-encoding oligonucleotide mixture, and then the ligation mixture was transformed into Escherichia coli DH5α competent cells (Invitrogen). .. Single colonies from the transformation were cultured, and plasmid DNAs were extracted by use of a Quick plasmid kit (Qiagen, Valencia, Calif.).

    Plasmid Preparation:

    Article Title: Designing, Construction and Expression of a Recombinant Fusion Protein Comprising the Hepatitis E Virus ORF2 and Rotavirus NSP4 in the Baculovirus Expression System
    Article Snippet: .. The pBlue Script II containing fusion truncated ORF2-NSP4 was transformed in E.coli DH5α, cultured in LB agar containing ampicillin (50 mg/L), then plasmid was extracted and double digested by BamHI and SalI restriction enzymes (Thermo Scientific, USA). .. Simultaneously, PfastBac1 plasmid was also double digested by BamHI and Sal1.

    Article Title: Immobilized Cobalt Affinity Chromatography Provides a Novel, Efficient Method for Herpes Simplex Virus Type 1 Gene Vector Purification
    Article Snippet: .. The oligonucleotides (10 pM) were mixed in phosphate-buffered saline (PBS), denatured at 100°C for 10 min, and then annealed for 1 h. BamHI-digested plasmid pTZ18UgBpK− DNA was dephosphorylated, gel purified, and then ligated with the annealed HAT-encoding oligonucleotide mixture, and then the ligation mixture was transformed into Escherichia coli DH5α competent cells (Invitrogen). .. Single colonies from the transformation were cultured, and plasmid DNAs were extracted by use of a Quick plasmid kit (Qiagen, Valencia, Calif.).

    Article Title: Construction of a Novel DNA Vaccine Candidate Encoding an HspX-PPE44-EsxV Fusion Antigen of Mycobacterium tuberculosis
    Article Snippet: .. Then the generated HspX-PPE44-EsxV, along with vector, was digested with BamHI and XbaI restriction enzymes and ligated into pcDNA3.1 (+) (Invitrogen, USA) in BamHI and XbaI restriction sites at the 5´and 3´ ends ( ). .. This plasmid was used to transform competent E. coli , Top-10 strain.

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    Thermo Fisher restriction enzymes bamhi
    FAR/FADO gene copy number determination in the selected TDR transformant. a Hybridization probe used for the Southern blot analysis; FADO fatty aldehyde deformylating oxygenase; TrpC_T terminator region of TrpC ; Tef1_P promoter region of Tef1 ; FAR fatty acyl-ACP/CoA reductase; <t>BamHI,</t> NdeI, PstI restriction enzyme cut sites. b Southern blot analysis. Lane 1 contains 1 kb <t>DNA</t> ladder (size in base pairs); lanes 2–4 shows the hybridization of the probe to the BamHI, PstI and NdeI digested gDNA of the TDR transformant; lanes 5–7 shows the hybridization of the probe to the BamHI, PstI and NdeI digested gDNA of the parent strain
    Restriction Enzymes Bamhi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzymes bamhi/product/Thermo Fisher
    Average 99 stars, based on 35 article reviews
    Price from $9.99 to $1999.99
    restriction enzymes bamhi - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    92
    Thermo Fisher bamh1 restriction enzymes
    FAR/FADO gene copy number determination in the selected TDR transformant. a Hybridization probe used for the Southern blot analysis; FADO fatty aldehyde deformylating oxygenase; TrpC_T terminator region of TrpC ; Tef1_P promoter region of Tef1 ; FAR fatty acyl-ACP/CoA reductase; <t>BamHI,</t> NdeI, PstI restriction enzyme cut sites. b Southern blot analysis. Lane 1 contains 1 kb <t>DNA</t> ladder (size in base pairs); lanes 2–4 shows the hybridization of the probe to the BamHI, PstI and NdeI digested gDNA of the TDR transformant; lanes 5–7 shows the hybridization of the probe to the BamHI, PstI and NdeI digested gDNA of the parent strain
    Bamh1 Restriction Enzymes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamh1 restriction enzymes/product/Thermo Fisher
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bamh1 restriction enzymes - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    Image Search Results


    FAR/FADO gene copy number determination in the selected TDR transformant. a Hybridization probe used for the Southern blot analysis; FADO fatty aldehyde deformylating oxygenase; TrpC_T terminator region of TrpC ; Tef1_P promoter region of Tef1 ; FAR fatty acyl-ACP/CoA reductase; BamHI, NdeI, PstI restriction enzyme cut sites. b Southern blot analysis. Lane 1 contains 1 kb DNA ladder (size in base pairs); lanes 2–4 shows the hybridization of the probe to the BamHI, PstI and NdeI digested gDNA of the TDR transformant; lanes 5–7 shows the hybridization of the probe to the BamHI, PstI and NdeI digested gDNA of the parent strain

    Journal: AMB Express

    Article Title: Alkane biosynthesis by Aspergillus carbonarius ITEM 5010 through heterologous expression of Synechococcus elongatus acyl-ACP/CoA reductase and aldehyde deformylating oxygenase genes

    doi: 10.1186/s13568-016-0321-x

    Figure Lengend Snippet: FAR/FADO gene copy number determination in the selected TDR transformant. a Hybridization probe used for the Southern blot analysis; FADO fatty aldehyde deformylating oxygenase; TrpC_T terminator region of TrpC ; Tef1_P promoter region of Tef1 ; FAR fatty acyl-ACP/CoA reductase; BamHI, NdeI, PstI restriction enzyme cut sites. b Southern blot analysis. Lane 1 contains 1 kb DNA ladder (size in base pairs); lanes 2–4 shows the hybridization of the probe to the BamHI, PstI and NdeI digested gDNA of the TDR transformant; lanes 5–7 shows the hybridization of the probe to the BamHI, PstI and NdeI digested gDNA of the parent strain

    Article Snippet: Genomic DNA of the transformants was extracted as described above and digested with restriction enzymes BamHI, PstI, and NdeI (all purchased from Thermo Scientific, Rockford, IL, USA).

    Techniques: Hybridization, Southern Blot

    Confirmation of Self-Ligated pP15A, kana R by Digestion Lane 2: digestion of pP15A, kana R vector with BamHI ; lane 3, xbaI; Lane 4, double digestion with BamHI-XbaI; Lane 5, undigested pP15A, kana R vector; Lanes 1 and 6, GeneRuler DNA ladder mix (Thermo Scientific, Waltham, MA, USA).

    Journal: Jundishapur Journal of Microbiology

    Article Title: Zinc Finger Nuclease: A New Approach to Overcome Beta-Lactam Antibiotic Resistance

    doi: 10.5812/jjm.29384

    Figure Lengend Snippet: Confirmation of Self-Ligated pP15A, kana R by Digestion Lane 2: digestion of pP15A, kana R vector with BamHI ; lane 3, xbaI; Lane 4, double digestion with BamHI-XbaI; Lane 5, undigested pP15A, kana R vector; Lanes 1 and 6, GeneRuler DNA ladder mix (Thermo Scientific, Waltham, MA, USA).

    Article Snippet: The pTZ57R plasmid was extracted using a GeneJET™ Plasmid Miniprep Kit (Thermo Scientific, Waltham, MA, USA) and was digested using EcoRI and BamHI restriction enzymes (Thermo Scientific, Waltham, MA, USA).

    Techniques: Plasmid Preparation