bamhi restriction enzymes  (TaKaRa)

 
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    Name:
    BamH I
    Description:
    G | GATC C C CTAG | G
    Catalog Number:
    1010b
    Price:
    None
    Size:
    50 000 Units
    Category:
    BamHI Restriction enzymes Cloning
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    Structured Review

    TaKaRa bamhi restriction enzymes
    G | GATC C C CTAG | G
    https://www.bioz.com/result/bamhi restriction enzymes/product/TaKaRa
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    bamhi restriction enzymes - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Transfection:

    Article Title: Therapeutic role of human hepatocyte growth factor (HGF) in treating hair loss
    Article Snippet: .. Materials E. coli DH5α, E. coli JM109 (Promega, WI, USA), BamHI and SalI (TaKaRa, Japan), pTARGET vector (Promega, Fitchburg, WI, USA), T4 DNA ligase (Promega, Fitchburg, WI, USA), TIAN quick Midi Purification Kit, TIANgel Midi Purification Kit, EndoFree Maxi Plasmid Kit (TIANGEN BIOTECH Co., Ltd.), Polymerase Chain Reaction (PCR) primer (Shanghai Sango Biotech Co., Ltd.), human skin fibroblasts (Academy of Military Medical Sciences), EntransterTM-in vivo Transfection Reagent (Engreen), Lipofectamine™ 2000 (Invitrogen), hHGF enzyme-linked immunosorbent assay (ELISA) Kit (R & D), rabbit anti-HGF monoclonal antibody, rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) monoclonal antibody, rabbit anti-β-catenin monoclonal antibody, Strept Avidin-biotin complex (SABC) kit and goat anti-rabbit IgG horseradish peroxidase (Wuhan Boster Biological Technology Co., Ltd.), β-catenin, HGF receptor (HGF-R) (Beijing Biosynthesis Biotechnology Co., Ltd.), Diaminobenzidine (DAB) and SP-9001 (ZSGB-BIO), Dulbecco’s modified eagle medium (DMEM) (Gibco), fetal bovine serum (FBS) (Hyclone), streptomycin sulfate and penicillin G sodium (Amresco), Boehringer DIG Luminescent Detection Kit (Boehringer–Mannheim), and Kodak X-Omat AR film (Kodak, Rochester) were commercially available and utilized according to manufacture’s protocol. .. The pEGFP-N1-hHGF was previously constructed in our laboratory ( ; ).

    Article Title: Development of TEM-1 β-lactamase based protein translocation assay for identification of Anaplasma phagocytophilum type IV secretion system effector proteins
    Article Snippet: .. Briefly, DNA fragment encoding full-length APH0215 was amplified by PCR using a pair of primers (APH0215-GFP-F and APH0215-GFP-R) (Table ) and A. phagocytophilum genomic DNA as template, followed by digestion with XhoI and BamHI, and ligation with eukaryotic expression vector pEGFP-N1 (Clontech), leading to the generation of plasmid pAPH0215-GFP. pAPH0215-GFP and pEGFP-N1 were then transfected into HeLa cells by Polyethylenimine MAX in EZ Trans transfection kit according to manufacturer’s instruction (Shanghai Life iLab Biotech Co, Shanghai, China), respectively. .. For IFA, the transfected cells were harvested at 36 h post-transfection, and subjected to fixation with 2% paraformaldehyde in PBS at RT for 30 min and washing with PBS for three times, followed by permeabilization and blocking with PS solution (PBS containing 0.8% BSA and 0.3% saponin) at RT for 10 min. Immunofluorescence labeling was performed using mouse monoclonal antibody against TGN38 (Clone B-6, Santa Cruz Biotechnology) and Alexa Fluor 555-conjugated goat anti-mouse IgG (Invitrogen).

    Amplification:

    Article Title: Structural and Functional Characterization of the Actin-1 Gene Promoter From the Antheraea pernyi (Lepidoptera: Saturniidae)
    Article Snippet: .. The amplified products were digested with HindIII and BamHI (Takara) and the large DNA restriction fragment purified by agarose gel electrophoresis. .. Plasmid pGFP-N2 (Clontech) was similarly digested and the smaller restriction fragment (GFP gene) was gel purified.

    Article Title: Efficient Gene Silencing Mediated by Tobacco Rattle Virus in an Emerging Model Plant Physalis
    Article Snippet: .. The amplified products and TRV2 were double digested with Nco I and BamH I (TaKaRa), and were then ligated using T4 DNA ligase (TaKaRa) to generate PfPDS -TRV2 , MPF2 -TRV2 , and MPF3 -TRV2 . ..

    Article Title: Development of TEM-1 β-lactamase based protein translocation assay for identification of Anaplasma phagocytophilum type IV secretion system effector proteins
    Article Snippet: .. Briefly, DNA fragment encoding full-length APH0215 was amplified by PCR using a pair of primers (APH0215-GFP-F and APH0215-GFP-R) (Table ) and A. phagocytophilum genomic DNA as template, followed by digestion with XhoI and BamHI, and ligation with eukaryotic expression vector pEGFP-N1 (Clontech), leading to the generation of plasmid pAPH0215-GFP. pAPH0215-GFP and pEGFP-N1 were then transfected into HeLa cells by Polyethylenimine MAX in EZ Trans transfection kit according to manufacturer’s instruction (Shanghai Life iLab Biotech Co, Shanghai, China), respectively. .. For IFA, the transfected cells were harvested at 36 h post-transfection, and subjected to fixation with 2% paraformaldehyde in PBS at RT for 30 min and washing with PBS for three times, followed by permeabilization and blocking with PS solution (PBS containing 0.8% BSA and 0.3% saponin) at RT for 10 min. Immunofluorescence labeling was performed using mouse monoclonal antibody against TGN38 (Clone B-6, Santa Cruz Biotechnology) and Alexa Fluor 555-conjugated goat anti-mouse IgG (Invitrogen).

    Agarose Gel Electrophoresis:

    Article Title: Structural and Functional Characterization of the Actin-1 Gene Promoter From the Antheraea pernyi (Lepidoptera: Saturniidae)
    Article Snippet: .. The amplified products were digested with HindIII and BamHI (Takara) and the large DNA restriction fragment purified by agarose gel electrophoresis. .. Plasmid pGFP-N2 (Clontech) was similarly digested and the smaller restriction fragment (GFP gene) was gel purified.

    Ligation:

    Article Title: Development of TEM-1 β-lactamase based protein translocation assay for identification of Anaplasma phagocytophilum type IV secretion system effector proteins
    Article Snippet: .. Briefly, DNA fragment encoding full-length APH0215 was amplified by PCR using a pair of primers (APH0215-GFP-F and APH0215-GFP-R) (Table ) and A. phagocytophilum genomic DNA as template, followed by digestion with XhoI and BamHI, and ligation with eukaryotic expression vector pEGFP-N1 (Clontech), leading to the generation of plasmid pAPH0215-GFP. pAPH0215-GFP and pEGFP-N1 were then transfected into HeLa cells by Polyethylenimine MAX in EZ Trans transfection kit according to manufacturer’s instruction (Shanghai Life iLab Biotech Co, Shanghai, China), respectively. .. For IFA, the transfected cells were harvested at 36 h post-transfection, and subjected to fixation with 2% paraformaldehyde in PBS at RT for 30 min and washing with PBS for three times, followed by permeabilization and blocking with PS solution (PBS containing 0.8% BSA and 0.3% saponin) at RT for 10 min. Immunofluorescence labeling was performed using mouse monoclonal antibody against TGN38 (Clone B-6, Santa Cruz Biotechnology) and Alexa Fluor 555-conjugated goat anti-mouse IgG (Invitrogen).

    Avidin-Biotin Assay:

    Article Title: Therapeutic role of human hepatocyte growth factor (HGF) in treating hair loss
    Article Snippet: .. Materials E. coli DH5α, E. coli JM109 (Promega, WI, USA), BamHI and SalI (TaKaRa, Japan), pTARGET vector (Promega, Fitchburg, WI, USA), T4 DNA ligase (Promega, Fitchburg, WI, USA), TIAN quick Midi Purification Kit, TIANgel Midi Purification Kit, EndoFree Maxi Plasmid Kit (TIANGEN BIOTECH Co., Ltd.), Polymerase Chain Reaction (PCR) primer (Shanghai Sango Biotech Co., Ltd.), human skin fibroblasts (Academy of Military Medical Sciences), EntransterTM-in vivo Transfection Reagent (Engreen), Lipofectamine™ 2000 (Invitrogen), hHGF enzyme-linked immunosorbent assay (ELISA) Kit (R & D), rabbit anti-HGF monoclonal antibody, rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) monoclonal antibody, rabbit anti-β-catenin monoclonal antibody, Strept Avidin-biotin complex (SABC) kit and goat anti-rabbit IgG horseradish peroxidase (Wuhan Boster Biological Technology Co., Ltd.), β-catenin, HGF receptor (HGF-R) (Beijing Biosynthesis Biotechnology Co., Ltd.), Diaminobenzidine (DAB) and SP-9001 (ZSGB-BIO), Dulbecco’s modified eagle medium (DMEM) (Gibco), fetal bovine serum (FBS) (Hyclone), streptomycin sulfate and penicillin G sodium (Amresco), Boehringer DIG Luminescent Detection Kit (Boehringer–Mannheim), and Kodak X-Omat AR film (Kodak, Rochester) were commercially available and utilized according to manufacture’s protocol. .. The pEGFP-N1-hHGF was previously constructed in our laboratory ( ; ).

    Purification:

    Article Title: Structural and Functional Characterization of the Actin-1 Gene Promoter From the Antheraea pernyi (Lepidoptera: Saturniidae)
    Article Snippet: .. The amplified products were digested with HindIII and BamHI (Takara) and the large DNA restriction fragment purified by agarose gel electrophoresis. .. Plasmid pGFP-N2 (Clontech) was similarly digested and the smaller restriction fragment (GFP gene) was gel purified.

    Article Title: Therapeutic role of human hepatocyte growth factor (HGF) in treating hair loss
    Article Snippet: .. Materials E. coli DH5α, E. coli JM109 (Promega, WI, USA), BamHI and SalI (TaKaRa, Japan), pTARGET vector (Promega, Fitchburg, WI, USA), T4 DNA ligase (Promega, Fitchburg, WI, USA), TIAN quick Midi Purification Kit, TIANgel Midi Purification Kit, EndoFree Maxi Plasmid Kit (TIANGEN BIOTECH Co., Ltd.), Polymerase Chain Reaction (PCR) primer (Shanghai Sango Biotech Co., Ltd.), human skin fibroblasts (Academy of Military Medical Sciences), EntransterTM-in vivo Transfection Reagent (Engreen), Lipofectamine™ 2000 (Invitrogen), hHGF enzyme-linked immunosorbent assay (ELISA) Kit (R & D), rabbit anti-HGF monoclonal antibody, rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) monoclonal antibody, rabbit anti-β-catenin monoclonal antibody, Strept Avidin-biotin complex (SABC) kit and goat anti-rabbit IgG horseradish peroxidase (Wuhan Boster Biological Technology Co., Ltd.), β-catenin, HGF receptor (HGF-R) (Beijing Biosynthesis Biotechnology Co., Ltd.), Diaminobenzidine (DAB) and SP-9001 (ZSGB-BIO), Dulbecco’s modified eagle medium (DMEM) (Gibco), fetal bovine serum (FBS) (Hyclone), streptomycin sulfate and penicillin G sodium (Amresco), Boehringer DIG Luminescent Detection Kit (Boehringer–Mannheim), and Kodak X-Omat AR film (Kodak, Rochester) were commercially available and utilized according to manufacture’s protocol. .. The pEGFP-N1-hHGF was previously constructed in our laboratory ( ; ).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Therapeutic role of human hepatocyte growth factor (HGF) in treating hair loss
    Article Snippet: .. Materials E. coli DH5α, E. coli JM109 (Promega, WI, USA), BamHI and SalI (TaKaRa, Japan), pTARGET vector (Promega, Fitchburg, WI, USA), T4 DNA ligase (Promega, Fitchburg, WI, USA), TIAN quick Midi Purification Kit, TIANgel Midi Purification Kit, EndoFree Maxi Plasmid Kit (TIANGEN BIOTECH Co., Ltd.), Polymerase Chain Reaction (PCR) primer (Shanghai Sango Biotech Co., Ltd.), human skin fibroblasts (Academy of Military Medical Sciences), EntransterTM-in vivo Transfection Reagent (Engreen), Lipofectamine™ 2000 (Invitrogen), hHGF enzyme-linked immunosorbent assay (ELISA) Kit (R & D), rabbit anti-HGF monoclonal antibody, rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) monoclonal antibody, rabbit anti-β-catenin monoclonal antibody, Strept Avidin-biotin complex (SABC) kit and goat anti-rabbit IgG horseradish peroxidase (Wuhan Boster Biological Technology Co., Ltd.), β-catenin, HGF receptor (HGF-R) (Beijing Biosynthesis Biotechnology Co., Ltd.), Diaminobenzidine (DAB) and SP-9001 (ZSGB-BIO), Dulbecco’s modified eagle medium (DMEM) (Gibco), fetal bovine serum (FBS) (Hyclone), streptomycin sulfate and penicillin G sodium (Amresco), Boehringer DIG Luminescent Detection Kit (Boehringer–Mannheim), and Kodak X-Omat AR film (Kodak, Rochester) were commercially available and utilized according to manufacture’s protocol. .. The pEGFP-N1-hHGF was previously constructed in our laboratory ( ; ).

    Polymerase Chain Reaction:

    Article Title: Therapeutic role of human hepatocyte growth factor (HGF) in treating hair loss
    Article Snippet: .. Materials E. coli DH5α, E. coli JM109 (Promega, WI, USA), BamHI and SalI (TaKaRa, Japan), pTARGET vector (Promega, Fitchburg, WI, USA), T4 DNA ligase (Promega, Fitchburg, WI, USA), TIAN quick Midi Purification Kit, TIANgel Midi Purification Kit, EndoFree Maxi Plasmid Kit (TIANGEN BIOTECH Co., Ltd.), Polymerase Chain Reaction (PCR) primer (Shanghai Sango Biotech Co., Ltd.), human skin fibroblasts (Academy of Military Medical Sciences), EntransterTM-in vivo Transfection Reagent (Engreen), Lipofectamine™ 2000 (Invitrogen), hHGF enzyme-linked immunosorbent assay (ELISA) Kit (R & D), rabbit anti-HGF monoclonal antibody, rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) monoclonal antibody, rabbit anti-β-catenin monoclonal antibody, Strept Avidin-biotin complex (SABC) kit and goat anti-rabbit IgG horseradish peroxidase (Wuhan Boster Biological Technology Co., Ltd.), β-catenin, HGF receptor (HGF-R) (Beijing Biosynthesis Biotechnology Co., Ltd.), Diaminobenzidine (DAB) and SP-9001 (ZSGB-BIO), Dulbecco’s modified eagle medium (DMEM) (Gibco), fetal bovine serum (FBS) (Hyclone), streptomycin sulfate and penicillin G sodium (Amresco), Boehringer DIG Luminescent Detection Kit (Boehringer–Mannheim), and Kodak X-Omat AR film (Kodak, Rochester) were commercially available and utilized according to manufacture’s protocol. .. The pEGFP-N1-hHGF was previously constructed in our laboratory ( ; ).

    Article Title: Development of TEM-1 β-lactamase based protein translocation assay for identification of Anaplasma phagocytophilum type IV secretion system effector proteins
    Article Snippet: .. Briefly, DNA fragment encoding full-length APH0215 was amplified by PCR using a pair of primers (APH0215-GFP-F and APH0215-GFP-R) (Table ) and A. phagocytophilum genomic DNA as template, followed by digestion with XhoI and BamHI, and ligation with eukaryotic expression vector pEGFP-N1 (Clontech), leading to the generation of plasmid pAPH0215-GFP. pAPH0215-GFP and pEGFP-N1 were then transfected into HeLa cells by Polyethylenimine MAX in EZ Trans transfection kit according to manufacturer’s instruction (Shanghai Life iLab Biotech Co, Shanghai, China), respectively. .. For IFA, the transfected cells were harvested at 36 h post-transfection, and subjected to fixation with 2% paraformaldehyde in PBS at RT for 30 min and washing with PBS for three times, followed by permeabilization and blocking with PS solution (PBS containing 0.8% BSA and 0.3% saponin) at RT for 10 min. Immunofluorescence labeling was performed using mouse monoclonal antibody against TGN38 (Clone B-6, Santa Cruz Biotechnology) and Alexa Fluor 555-conjugated goat anti-mouse IgG (Invitrogen).

    Expressing:

    Article Title: Development of TEM-1 β-lactamase based protein translocation assay for identification of Anaplasma phagocytophilum type IV secretion system effector proteins
    Article Snippet: .. Briefly, DNA fragment encoding full-length APH0215 was amplified by PCR using a pair of primers (APH0215-GFP-F and APH0215-GFP-R) (Table ) and A. phagocytophilum genomic DNA as template, followed by digestion with XhoI and BamHI, and ligation with eukaryotic expression vector pEGFP-N1 (Clontech), leading to the generation of plasmid pAPH0215-GFP. pAPH0215-GFP and pEGFP-N1 were then transfected into HeLa cells by Polyethylenimine MAX in EZ Trans transfection kit according to manufacturer’s instruction (Shanghai Life iLab Biotech Co, Shanghai, China), respectively. .. For IFA, the transfected cells were harvested at 36 h post-transfection, and subjected to fixation with 2% paraformaldehyde in PBS at RT for 30 min and washing with PBS for three times, followed by permeabilization and blocking with PS solution (PBS containing 0.8% BSA and 0.3% saponin) at RT for 10 min. Immunofluorescence labeling was performed using mouse monoclonal antibody against TGN38 (Clone B-6, Santa Cruz Biotechnology) and Alexa Fluor 555-conjugated goat anti-mouse IgG (Invitrogen).

    Modification:

    Article Title: Therapeutic role of human hepatocyte growth factor (HGF) in treating hair loss
    Article Snippet: .. Materials E. coli DH5α, E. coli JM109 (Promega, WI, USA), BamHI and SalI (TaKaRa, Japan), pTARGET vector (Promega, Fitchburg, WI, USA), T4 DNA ligase (Promega, Fitchburg, WI, USA), TIAN quick Midi Purification Kit, TIANgel Midi Purification Kit, EndoFree Maxi Plasmid Kit (TIANGEN BIOTECH Co., Ltd.), Polymerase Chain Reaction (PCR) primer (Shanghai Sango Biotech Co., Ltd.), human skin fibroblasts (Academy of Military Medical Sciences), EntransterTM-in vivo Transfection Reagent (Engreen), Lipofectamine™ 2000 (Invitrogen), hHGF enzyme-linked immunosorbent assay (ELISA) Kit (R & D), rabbit anti-HGF monoclonal antibody, rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) monoclonal antibody, rabbit anti-β-catenin monoclonal antibody, Strept Avidin-biotin complex (SABC) kit and goat anti-rabbit IgG horseradish peroxidase (Wuhan Boster Biological Technology Co., Ltd.), β-catenin, HGF receptor (HGF-R) (Beijing Biosynthesis Biotechnology Co., Ltd.), Diaminobenzidine (DAB) and SP-9001 (ZSGB-BIO), Dulbecco’s modified eagle medium (DMEM) (Gibco), fetal bovine serum (FBS) (Hyclone), streptomycin sulfate and penicillin G sodium (Amresco), Boehringer DIG Luminescent Detection Kit (Boehringer–Mannheim), and Kodak X-Omat AR film (Kodak, Rochester) were commercially available and utilized according to manufacture’s protocol. .. The pEGFP-N1-hHGF was previously constructed in our laboratory ( ; ).

    Recombinant:

    Article Title: Characterization of the novel In1059 harbouring VIM gene cassette
    Article Snippet: .. The plasmids were digested with BamHI (TaKaRa, Dalian, China), and the six integron-harbouring recombinant plasmids were electrophoretically resolved to generate genetic maps. .. To characterize the recombinant plasmid-encoding integrons from the above six isolates, we digested the plasmids with BamHI and ligated the relevant fragments to the pMD19-T cloning vector (TaKaRa, Dalian, China), and then transformed the ligation mixture into E. coli TOP10 host bacteria.

    Plasmid Preparation:

    Article Title: Dissemination of blaOXA-23 in Acinetobacter spp. in China: Main Roles of Conjugative Plasmid pAZJ221 and Transposon Tn2009
    Article Snippet: .. In order to identify plasmid patterns, genomic DNA of isolates with the plasmid-borne bla OXA-23 gene, digested with BamHI or EcoRI (TaKaRa, Japan), was separated and hybridized, as described above. .. Furthermore, genomic DNA digested by ApaI (TaKaRa, Japan) was electrophoresed and then hybridized, as described above, to reveal the chromosomal locations of the bla OXA-23 gene through comparison of hybridization signals with S1-PFGE.

    Article Title: Therapeutic role of human hepatocyte growth factor (HGF) in treating hair loss
    Article Snippet: .. Materials E. coli DH5α, E. coli JM109 (Promega, WI, USA), BamHI and SalI (TaKaRa, Japan), pTARGET vector (Promega, Fitchburg, WI, USA), T4 DNA ligase (Promega, Fitchburg, WI, USA), TIAN quick Midi Purification Kit, TIANgel Midi Purification Kit, EndoFree Maxi Plasmid Kit (TIANGEN BIOTECH Co., Ltd.), Polymerase Chain Reaction (PCR) primer (Shanghai Sango Biotech Co., Ltd.), human skin fibroblasts (Academy of Military Medical Sciences), EntransterTM-in vivo Transfection Reagent (Engreen), Lipofectamine™ 2000 (Invitrogen), hHGF enzyme-linked immunosorbent assay (ELISA) Kit (R & D), rabbit anti-HGF monoclonal antibody, rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) monoclonal antibody, rabbit anti-β-catenin monoclonal antibody, Strept Avidin-biotin complex (SABC) kit and goat anti-rabbit IgG horseradish peroxidase (Wuhan Boster Biological Technology Co., Ltd.), β-catenin, HGF receptor (HGF-R) (Beijing Biosynthesis Biotechnology Co., Ltd.), Diaminobenzidine (DAB) and SP-9001 (ZSGB-BIO), Dulbecco’s modified eagle medium (DMEM) (Gibco), fetal bovine serum (FBS) (Hyclone), streptomycin sulfate and penicillin G sodium (Amresco), Boehringer DIG Luminescent Detection Kit (Boehringer–Mannheim), and Kodak X-Omat AR film (Kodak, Rochester) were commercially available and utilized according to manufacture’s protocol. .. The pEGFP-N1-hHGF was previously constructed in our laboratory ( ; ).

    Article Title: Development of TEM-1 β-lactamase based protein translocation assay for identification of Anaplasma phagocytophilum type IV secretion system effector proteins
    Article Snippet: .. Briefly, DNA fragment encoding full-length APH0215 was amplified by PCR using a pair of primers (APH0215-GFP-F and APH0215-GFP-R) (Table ) and A. phagocytophilum genomic DNA as template, followed by digestion with XhoI and BamHI, and ligation with eukaryotic expression vector pEGFP-N1 (Clontech), leading to the generation of plasmid pAPH0215-GFP. pAPH0215-GFP and pEGFP-N1 were then transfected into HeLa cells by Polyethylenimine MAX in EZ Trans transfection kit according to manufacturer’s instruction (Shanghai Life iLab Biotech Co, Shanghai, China), respectively. .. For IFA, the transfected cells were harvested at 36 h post-transfection, and subjected to fixation with 2% paraformaldehyde in PBS at RT for 30 min and washing with PBS for three times, followed by permeabilization and blocking with PS solution (PBS containing 0.8% BSA and 0.3% saponin) at RT for 10 min. Immunofluorescence labeling was performed using mouse monoclonal antibody against TGN38 (Clone B-6, Santa Cruz Biotechnology) and Alexa Fluor 555-conjugated goat anti-mouse IgG (Invitrogen).

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  • 85
    TaKaRa reagents restriction endonuclease enzyme bamhi
    Transcription and expression of HCV core-IFN-α2a recombinant viruses. (A) Identification of pFBD-H1 and pFBD-H2. M: 1Kb Plus DNA ladder; pFBD-H1 and pFBD-H2 samples were digested by <t>BamHI</t> and <t>EcoRI.</t> (B) Identification of pFBD-H3 and pFBD-H4. M: 1Kb Plus DNA ladder; pFBD-H3 and pFBD-H4 samples were digested by BamHI and EcoRI. (C) RT-PCR results of HCV core gene in recombination viruses infect cells. Total RNA was isolated from Sf9 infected with vAcH1, vAcH2, vAcH3, or vAcH4. cDNA was synthesized with SuperScript First Strand Synthesis kit (Invitrogen) with 0.5 to 1.0 μg RNA according to the manufacturer’s instructions. Quantitative RT-PCR reactions were carried out using SYBR Green PCR master mix reagents on an ABI 7500 Fast Real-Time PCR System (Applied Biosystems). (D) Expression of HCV core-IFN-α2a fusion protein in recombinant virus infected cells. M: protein marker. Cells were harvested at 72 h post-infection (hpi) and lysed in SDS-PAGE loading buffer. Twenty micrograms of total protein was separated by SDS-PAGE and subjected to Western blot assay.
    Reagents Restriction Endonuclease Enzyme Bamhi, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reagents restriction endonuclease enzyme bamhi/product/TaKaRa
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    reagents restriction endonuclease enzyme bamhi - by Bioz Stars, 2020-08
    85/100 stars
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    99
    TaKaRa restriction enzymes bamh i
    Design of inserting <t>DNA</t> fragments encoding short peptides and DNA sequencing. (a, b) BSP-based design for both plus and minus strands of huZP4 gene fragments encoding 18/8mer peptides, and (c) sequencing result of plus strand inserted fragment encoding P31. Pink color in all boxes indicates the paired bp in each double strand and overlapping regions of 10/7 aa between designed P31-32 and P115-116. Other colors indicate various paired bp in four of double strands. The underlines in Fig 2c indicate <t>BamH</t> I and Sal I restriction sites.
    Restriction Enzymes Bamh I, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzymes bamh i/product/TaKaRa
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    restriction enzymes bamh i - by Bioz Stars, 2020-08
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    88
    TaKaRa bamhi sali restriction enzymes
    Design of inserting <t>DNA</t> fragments encoding short peptides and DNA sequencing. (a, b) BSP-based design for both plus and minus strands of huZP4 gene fragments encoding 18/8mer peptides, and (c) sequencing result of plus strand inserted fragment encoding P31. Pink color in all boxes indicates the paired bp in each double strand and overlapping regions of 10/7 aa between designed P31-32 and P115-116. Other colors indicate various paired bp in four of double strands. The underlines in Fig 2c indicate <t>BamH</t> I and Sal I restriction sites.
    Bamhi Sali Restriction Enzymes, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamhi sali restriction enzymes/product/TaKaRa
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bamhi sali restriction enzymes - by Bioz Stars, 2020-08
    88/100 stars
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    99
    TaKaRa restriction enzymes
    Design of inserting <t>DNA</t> fragments encoding short peptides and DNA sequencing. (a, b) BSP-based design for both plus and minus strands of huZP4 gene fragments encoding 18/8mer peptides, and (c) sequencing result of plus strand inserted fragment encoding P31. Pink color in all boxes indicates the paired bp in each double strand and overlapping regions of 10/7 aa between designed P31-32 and P115-116. Other colors indicate various paired bp in four of double strands. The underlines in Fig 2c indicate <t>BamH</t> I and Sal I restriction sites.
    Restriction Enzymes, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzymes/product/TaKaRa
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    restriction enzymes - by Bioz Stars, 2020-08
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    Transcription and expression of HCV core-IFN-α2a recombinant viruses. (A) Identification of pFBD-H1 and pFBD-H2. M: 1Kb Plus DNA ladder; pFBD-H1 and pFBD-H2 samples were digested by BamHI and EcoRI. (B) Identification of pFBD-H3 and pFBD-H4. M: 1Kb Plus DNA ladder; pFBD-H3 and pFBD-H4 samples were digested by BamHI and EcoRI. (C) RT-PCR results of HCV core gene in recombination viruses infect cells. Total RNA was isolated from Sf9 infected with vAcH1, vAcH2, vAcH3, or vAcH4. cDNA was synthesized with SuperScript First Strand Synthesis kit (Invitrogen) with 0.5 to 1.0 μg RNA according to the manufacturer’s instructions. Quantitative RT-PCR reactions were carried out using SYBR Green PCR master mix reagents on an ABI 7500 Fast Real-Time PCR System (Applied Biosystems). (D) Expression of HCV core-IFN-α2a fusion protein in recombinant virus infected cells. M: protein marker. Cells were harvested at 72 h post-infection (hpi) and lysed in SDS-PAGE loading buffer. Twenty micrograms of total protein was separated by SDS-PAGE and subjected to Western blot assay.

    Journal: Nanoscale Research Letters

    Article Title: Self-assembled HCV core virus-like particles targeted and inhibited tumor cell migration and invasion

    doi: 10.1186/1556-276X-8-401

    Figure Lengend Snippet: Transcription and expression of HCV core-IFN-α2a recombinant viruses. (A) Identification of pFBD-H1 and pFBD-H2. M: 1Kb Plus DNA ladder; pFBD-H1 and pFBD-H2 samples were digested by BamHI and EcoRI. (B) Identification of pFBD-H3 and pFBD-H4. M: 1Kb Plus DNA ladder; pFBD-H3 and pFBD-H4 samples were digested by BamHI and EcoRI. (C) RT-PCR results of HCV core gene in recombination viruses infect cells. Total RNA was isolated from Sf9 infected with vAcH1, vAcH2, vAcH3, or vAcH4. cDNA was synthesized with SuperScript First Strand Synthesis kit (Invitrogen) with 0.5 to 1.0 μg RNA according to the manufacturer’s instructions. Quantitative RT-PCR reactions were carried out using SYBR Green PCR master mix reagents on an ABI 7500 Fast Real-Time PCR System (Applied Biosystems). (D) Expression of HCV core-IFN-α2a fusion protein in recombinant virus infected cells. M: protein marker. Cells were harvested at 72 h post-infection (hpi) and lysed in SDS-PAGE loading buffer. Twenty micrograms of total protein was separated by SDS-PAGE and subjected to Western blot assay.

    Article Snippet: Reagents Restriction endonuclease enzyme BamHI, EcoRI, SalI, nucleic acid molecular weight marker, DNA polymerase Pfu, DNA Marker, Gel extraction kit, and T4 DNA ligase were from TaKaRa (Shiga, Japan).

    Techniques: Expressing, Recombinant, Reverse Transcription Polymerase Chain Reaction, Isolation, Infection, Synthesized, Quantitative RT-PCR, SYBR Green Assay, Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Marker, SDS Page, Western Blot

    RGD-core-IFN-α2a fusion proteins bind breast cancer cells MDA-MB231 in vitro. (A) Recombinant bacmid constructs, showing the strategy for insertion of the gene cassettes into the polyhedrin locus of the AcMNPV bacmid. RGD-HCV core was fused with IFN-α2a. Both cassettes depicted were inserted into the attb site (indicated by the right and left insertion sites, Tn7R and Tn7L) in the polyhedrin locus by Tn-based transposition and generated the recombinant Bacmid: AcH1, AcH2, AcH3, and AcH4. (B) Identification of pH1 and pH2. M: 1Kb Plus DNA ladder; pH1 and pH2 samples were digested by BamHI and EcoRI. (C) Identification of pH3 and pH4. M: O’Gene Ruler 1Kb DNA ladder; pH3 and pH4 samples were digested by BamHI and EcoRI. (D) Purification of RGD-core-IFN-α2a fusion protein. M: protein marker; 1: His-H1; 2: His-H2; 3: His-H3; 4: His-H4. The recombinant bacmids AcH1, AcH2, AcH3, and AcH4 were introduced by transfection into Sf9 cells to produce the recombinant proteins His-H1, His-H2, His-H3, and His-H4. The fusion proteins were purified from the supernatants of cell lysates using Ni-NTA affinity resin. (E , G) Electron micrograph images and Western blotting result of VLP H1. Purified VLPs were attached onto a carbon-coated grid for 5 min at room temperature. The grid was rinsed with distilled water and stained with 1% phosphotungstic acid for 3 min before air drying on filter paper. The specimens were viewed using a Tecnai G2 transmission electron microscopy at 75 keV. For Western blot, 10 μg purified VLPs were separated by SDS-PAGE electrophoresis and subjected to Western blot assay. (F , H) Electron micrograph images and Western blotting result of VLP H2. (I) RGD-core-IFN-α2a fusion protein bind with breast cancer cells MDA-MB231. Then, 0.2, 0.5, 2, 5, and 10 μM fusion proteins His-H1, His-H2, His-H3, and His-H4 were co-incubated with MDA-MB231 at 37° under 5% CO 2 . After 2 h, the cells were washed three times with PBS, and green fluorescence was observed under the fluorescence microscope. Scale bar = 100 μm.

    Journal: Nanoscale Research Letters

    Article Title: Self-assembled HCV core virus-like particles targeted and inhibited tumor cell migration and invasion

    doi: 10.1186/1556-276X-8-401

    Figure Lengend Snippet: RGD-core-IFN-α2a fusion proteins bind breast cancer cells MDA-MB231 in vitro. (A) Recombinant bacmid constructs, showing the strategy for insertion of the gene cassettes into the polyhedrin locus of the AcMNPV bacmid. RGD-HCV core was fused with IFN-α2a. Both cassettes depicted were inserted into the attb site (indicated by the right and left insertion sites, Tn7R and Tn7L) in the polyhedrin locus by Tn-based transposition and generated the recombinant Bacmid: AcH1, AcH2, AcH3, and AcH4. (B) Identification of pH1 and pH2. M: 1Kb Plus DNA ladder; pH1 and pH2 samples were digested by BamHI and EcoRI. (C) Identification of pH3 and pH4. M: O’Gene Ruler 1Kb DNA ladder; pH3 and pH4 samples were digested by BamHI and EcoRI. (D) Purification of RGD-core-IFN-α2a fusion protein. M: protein marker; 1: His-H1; 2: His-H2; 3: His-H3; 4: His-H4. The recombinant bacmids AcH1, AcH2, AcH3, and AcH4 were introduced by transfection into Sf9 cells to produce the recombinant proteins His-H1, His-H2, His-H3, and His-H4. The fusion proteins were purified from the supernatants of cell lysates using Ni-NTA affinity resin. (E , G) Electron micrograph images and Western blotting result of VLP H1. Purified VLPs were attached onto a carbon-coated grid for 5 min at room temperature. The grid was rinsed with distilled water and stained with 1% phosphotungstic acid for 3 min before air drying on filter paper. The specimens were viewed using a Tecnai G2 transmission electron microscopy at 75 keV. For Western blot, 10 μg purified VLPs were separated by SDS-PAGE electrophoresis and subjected to Western blot assay. (F , H) Electron micrograph images and Western blotting result of VLP H2. (I) RGD-core-IFN-α2a fusion protein bind with breast cancer cells MDA-MB231. Then, 0.2, 0.5, 2, 5, and 10 μM fusion proteins His-H1, His-H2, His-H3, and His-H4 were co-incubated with MDA-MB231 at 37° under 5% CO 2 . After 2 h, the cells were washed three times with PBS, and green fluorescence was observed under the fluorescence microscope. Scale bar = 100 μm.

    Article Snippet: Reagents Restriction endonuclease enzyme BamHI, EcoRI, SalI, nucleic acid molecular weight marker, DNA polymerase Pfu, DNA Marker, Gel extraction kit, and T4 DNA ligase were from TaKaRa (Shiga, Japan).

    Techniques: Multiple Displacement Amplification, In Vitro, Recombinant, Construct, Generated, Purification, Marker, Transfection, Western Blot, Staining, Transmission Assay, Electron Microscopy, SDS Page, Electrophoresis, Incubation, Fluorescence, Microscopy

    Design of inserting DNA fragments encoding short peptides and DNA sequencing. (a, b) BSP-based design for both plus and minus strands of huZP4 gene fragments encoding 18/8mer peptides, and (c) sequencing result of plus strand inserted fragment encoding P31. Pink color in all boxes indicates the paired bp in each double strand and overlapping regions of 10/7 aa between designed P31-32 and P115-116. Other colors indicate various paired bp in four of double strands. The underlines in Fig 2c indicate BamH I and Sal I restriction sites.

    Journal: PLoS ONE

    Article Title: A simpler and more cost-effective peptide biosynthetic method using the truncated GST as carrier for epitope mapping

    doi: 10.1371/journal.pone.0186097

    Figure Lengend Snippet: Design of inserting DNA fragments encoding short peptides and DNA sequencing. (a, b) BSP-based design for both plus and minus strands of huZP4 gene fragments encoding 18/8mer peptides, and (c) sequencing result of plus strand inserted fragment encoding P31. Pink color in all boxes indicates the paired bp in each double strand and overlapping regions of 10/7 aa between designed P31-32 and P115-116. Other colors indicate various paired bp in four of double strands. The underlines in Fig 2c indicate BamH I and Sal I restriction sites.

    Article Snippet: Other reagents and materials DNA ligase, restriction enzymes BamH I and Sal I were purchased from Takara Co., Ltd (Dalian, China).

    Techniques: DNA Sequencing, Sequencing

    Weak antigenic range of cell proteins in Western blot and schematic diagram of relevant plasmids. (a) SDS-PAGE analysis of expressed GST170-8mer peptides and (b) Western blotting with rabbit antiserum generated against the r-segment b (aa residues 177–348) of human zona pellucida glycoprotein-3 (huZP3b), and (c-e) Schematic diagram of pXXGST-1, pXXGST-2 and pXXGST-3. The arrow indicates the expressed various 8mer peptides fused with GST170 protein (subpanel a), and the red box shows a maximum weak antigenic area (subpanel b). The boldfaced letters in cloning site and other colors in pXXGST-1 and -3 indicate enzyme restriction sites of BamH I and Sal I, and gene fragments encoding GST188 and GST188-E7 proteins, respectively.

    Journal: PLoS ONE

    Article Title: A simpler and more cost-effective peptide biosynthetic method using the truncated GST as carrier for epitope mapping

    doi: 10.1371/journal.pone.0186097

    Figure Lengend Snippet: Weak antigenic range of cell proteins in Western blot and schematic diagram of relevant plasmids. (a) SDS-PAGE analysis of expressed GST170-8mer peptides and (b) Western blotting with rabbit antiserum generated against the r-segment b (aa residues 177–348) of human zona pellucida glycoprotein-3 (huZP3b), and (c-e) Schematic diagram of pXXGST-1, pXXGST-2 and pXXGST-3. The arrow indicates the expressed various 8mer peptides fused with GST170 protein (subpanel a), and the red box shows a maximum weak antigenic area (subpanel b). The boldfaced letters in cloning site and other colors in pXXGST-1 and -3 indicate enzyme restriction sites of BamH I and Sal I, and gene fragments encoding GST188 and GST188-E7 proteins, respectively.

    Article Snippet: Other reagents and materials DNA ligase, restriction enzymes BamH I and Sal I were purchased from Takara Co., Ltd (Dalian, China).

    Techniques: Western Blot, SDS Page, Generated, Clone Assay