Structured Review

TaKaRa bamhi restriction enzymes
Bamhi Restriction Enzymes, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bamhi restriction enzymes/product/TaKaRa
Average 95 stars, based on 6 article reviews
Price from $9.99 to $1999.99
bamhi restriction enzymes - by Bioz Stars, 2020-01
95/100 stars

Images

Related Articles

Clone Assay:

Article Title: Protein phosphatase 2A is requisite for the function of regulatory T cells
Article Snippet: .. For lentiviral overexpression of SGMS1 and SET, the full coding sequences of murine Sgms1 and Set were cloned from cDNA generated from murine CD4+ T cells using the following primers: Sgms1-cloning-for 5′-ATCTGGAATTCGCAAGCTGGGGGTACTGAAT-3′ Sgms1-cloning-rev 5′-ATTCGGGATCCCATTCCCTAGTCGGCAGAGC-3′ SET-cloning-for 5′-ATCTGGAATTCCTGTCTCCCGGTCATCTCCC-3′ SET-cloning-rev 5′-ATTCGGGATCCAGGGAGGAAAGGACTGCAAC-3′5′- They were then incorporated using EcoRI and BamHI restriction enzymes into the pLVX-EF1α-IRES-mCherry Lentiviral Vector (cat#: 631987, Clontech Laboratories) and viral particles were generated in HEK293FT cells using the ViraPower Lentiviral Packaging Mix (cat#: K4975-00, Life Technologies). .. Viral supernatants were concentrated 100-fold with Lenti-X™ Concentrator (cat#: 631231, Clontech Laboratories).

Flow Cytometry:

Article Title: Protein phosphatase 2A is requisite for the function of regulatory T cells
Article Snippet: For lentiviral overexpression of SGMS1 and SET, the full coding sequences of murine Sgms1 and Set were cloned from cDNA generated from murine CD4+ T cells using the following primers: Sgms1-cloning-for 5′-ATCTGGAATTCGCAAGCTGGGGGTACTGAAT-3′ Sgms1-cloning-rev 5′-ATTCGGGATCCCATTCCCTAGTCGGCAGAGC-3′ SET-cloning-for 5′-ATCTGGAATTCCTGTCTCCCGGTCATCTCCC-3′ SET-cloning-rev 5′-ATTCGGGATCCAGGGAGGAAAGGACTGCAAC-3′5′- They were then incorporated using EcoRI and BamHI restriction enzymes into the pLVX-EF1α-IRES-mCherry Lentiviral Vector (cat#: 631987, Clontech Laboratories) and viral particles were generated in HEK293FT cells using the ViraPower Lentiviral Packaging Mix (cat#: K4975-00, Life Technologies). .. Downstream flow cytometry analysis of the infected cells was performed by gating on mCherry+ T cells.

shRNA:

Article Title: Protein phosphatase 2A is requisite for the function of regulatory T cells
Article Snippet: Plasmid, retroviral and lentiviral infections For SET-silencing experiments, the psi-mU6 mCherry-expressing vector was used harboring a SET-specific shRNA or a scrambled control (purchased from Genecopeia). .. For lentiviral overexpression of SGMS1 and SET, the full coding sequences of murine Sgms1 and Set were cloned from cDNA generated from murine CD4+ T cells using the following primers: Sgms1-cloning-for 5′-ATCTGGAATTCGCAAGCTGGGGGTACTGAAT-3′ Sgms1-cloning-rev 5′-ATTCGGGATCCCATTCCCTAGTCGGCAGAGC-3′ SET-cloning-for 5′-ATCTGGAATTCCTGTCTCCCGGTCATCTCCC-3′ SET-cloning-rev 5′-ATTCGGGATCCAGGGAGGAAAGGACTGCAAC-3′5′- They were then incorporated using EcoRI and BamHI restriction enzymes into the pLVX-EF1α-IRES-mCherry Lentiviral Vector (cat#: 631987, Clontech Laboratories) and viral particles were generated in HEK293FT cells using the ViraPower Lentiviral Packaging Mix (cat#: K4975-00, Life Technologies).

Mutagenesis:

Article Title: Mechanical Stretch Inhibits MicroRNA499 via p53 to Regulate Calcineurin-A Expression in Rat Cardiomyocytes
Article Snippet: .. The 200bp amplified product was digested using EcoRI and BamHI restriction enzymes and ligated into pmR-ZsGreen1 plasmid vector (coexpression miR499 and green fluorescent protein, Clontech Laboratories, Mountain View, CA, USA). (B) MiR499 antagomir, and (C) mutant miR499 precursor construct was designed (Applied Biosystems) and ligated into the same plasmid vector as miR499. .. The constructed plasmid was transfected into cultured cardiomyoctyes or left ventricular myocardium using a low pressure-accelerated gene gun (Bioware Technologies, Taipei, Taiwan) per manufacturer instructions.

Article Title: Angiotensin II Downregulates MicroRNA-145 to Regulate Kruppel-like Factor 4 and Myocardin Expression in Human Coronary Arterial Smooth Muscle Cells under High Glucose Conditions
Article Snippet: The 199-bp amplified product was digested with EcoRI and BamHI restriction enzymes and ligated into pmR-ZsGreen1 plasmid vector (coexpression miR-145 and green fluorescent protein) (Clontech Laboratories) digested with the same enzymes. .. Antagomir-145 and mutant-miR-145 precursor construct was generated in pmR-ZsGreen1 plasmid vector.

Construct:

Article Title: Mechanical Stretch Inhibits MicroRNA499 via p53 to Regulate Calcineurin-A Expression in Rat Cardiomyocytes
Article Snippet: .. The 200bp amplified product was digested using EcoRI and BamHI restriction enzymes and ligated into pmR-ZsGreen1 plasmid vector (coexpression miR499 and green fluorescent protein, Clontech Laboratories, Mountain View, CA, USA). (B) MiR499 antagomir, and (C) mutant miR499 precursor construct was designed (Applied Biosystems) and ligated into the same plasmid vector as miR499. .. The constructed plasmid was transfected into cultured cardiomyoctyes or left ventricular myocardium using a low pressure-accelerated gene gun (Bioware Technologies, Taipei, Taiwan) per manufacturer instructions.

Article Title: Angiotensin II Downregulates MicroRNA-145 to Regulate Kruppel-like Factor 4 and Myocardin Expression in Human Coronary Arterial Smooth Muscle Cells under High Glucose Conditions
Article Snippet: A 88bp miR-145 precursor construct was generated in the following manner. .. The 199-bp amplified product was digested with EcoRI and BamHI restriction enzymes and ligated into pmR-ZsGreen1 plasmid vector (coexpression miR-145 and green fluorescent protein) (Clontech Laboratories) digested with the same enzymes.

Article Title: Cloning and Expression Characteristics of the Pig Stra8 Gene
Article Snippet: The PCR primers used were 5'-CC AAGCTT ATGGCAACCTCTGGAGAAGG-3' (forward) and 5'-CGC GGATCC CAGATCTTCGTCGTCAAATG-3' (reverse) (Hind III and BamHI restriction sites underlined), which were constructed based on the sequence obtained above. .. The resulting PCR products and EGFP-N1 vector (Clontech, Mountain View, CA, USA) were double digested with Hind III and BamHI restriction enzymes and ligated at 16 °C for 16 h using T4 ligase (Takara).

Transfection:

Article Title: Mechanical Stretch Inhibits MicroRNA499 via p53 to Regulate Calcineurin-A Expression in Rat Cardiomyocytes
Article Snippet: The 200bp amplified product was digested using EcoRI and BamHI restriction enzymes and ligated into pmR-ZsGreen1 plasmid vector (coexpression miR499 and green fluorescent protein, Clontech Laboratories, Mountain View, CA, USA). (B) MiR499 antagomir, and (C) mutant miR499 precursor construct was designed (Applied Biosystems) and ligated into the same plasmid vector as miR499. .. The constructed plasmid was transfected into cultured cardiomyoctyes or left ventricular myocardium using a low pressure-accelerated gene gun (Bioware Technologies, Taipei, Taiwan) per manufacturer instructions.

Article Title: Angiotensin II Downregulates MicroRNA-145 to Regulate Kruppel-like Factor 4 and Myocardin Expression in Human Coronary Arterial Smooth Muscle Cells under High Glucose Conditions
Article Snippet: The 199-bp amplified product was digested with EcoRI and BamHI restriction enzymes and ligated into pmR-ZsGreen1 plasmid vector (coexpression miR-145 and green fluorescent protein) (Clontech Laboratories) digested with the same enzymes. .. The constructed plasmid was transfected into cells using a low pressure-accelerated gene gun (Bioware Technologies) following the protocol from the manufacturer.

Cell Culture:

Article Title: Mechanical Stretch Inhibits MicroRNA499 via p53 to Regulate Calcineurin-A Expression in Rat Cardiomyocytes
Article Snippet: Paragraph title: Construction and delivery of miR499, antagomir499, and mutant-miR499 expression vector into cultured cardiomyocytes and ventricular myocardium ... The 200bp amplified product was digested using EcoRI and BamHI restriction enzymes and ligated into pmR-ZsGreen1 plasmid vector (coexpression miR499 and green fluorescent protein, Clontech Laboratories, Mountain View, CA, USA). (B) MiR499 antagomir, and (C) mutant miR499 precursor construct was designed (Applied Biosystems) and ligated into the same plasmid vector as miR499.

Cytometry:

Article Title: Protein phosphatase 2A is requisite for the function of regulatory T cells
Article Snippet: For lentiviral overexpression of SGMS1 and SET, the full coding sequences of murine Sgms1 and Set were cloned from cDNA generated from murine CD4+ T cells using the following primers: Sgms1-cloning-for 5′-ATCTGGAATTCGCAAGCTGGGGGTACTGAAT-3′ Sgms1-cloning-rev 5′-ATTCGGGATCCCATTCCCTAGTCGGCAGAGC-3′ SET-cloning-for 5′-ATCTGGAATTCCTGTCTCCCGGTCATCTCCC-3′ SET-cloning-rev 5′-ATTCGGGATCCAGGGAGGAAAGGACTGCAAC-3′5′- They were then incorporated using EcoRI and BamHI restriction enzymes into the pLVX-EF1α-IRES-mCherry Lentiviral Vector (cat#: 631987, Clontech Laboratories) and viral particles were generated in HEK293FT cells using the ViraPower Lentiviral Packaging Mix (cat#: K4975-00, Life Technologies). .. Downstream flow cytometry analysis of the infected cells was performed by gating on mCherry+ T cells.

Sequencing:

Article Title: Angiotensin II Downregulates MicroRNA-145 to Regulate Kruppel-like Factor 4 and Myocardin Expression in Human Coronary Arterial Smooth Muscle Cells under High Glucose Conditions
Article Snippet: The 199-bp amplified product was digested with EcoRI and BamHI restriction enzymes and ligated into pmR-ZsGreen1 plasmid vector (coexpression miR-145 and green fluorescent protein) (Clontech Laboratories) digested with the same enzymes. .. The mutant miR-145 precursor sequence was mutated from GGGGG of the miR-145 precursor construct to CCCCC.

Article Title: Cloning and Expression Characteristics of the Pig Stra8 Gene
Article Snippet: The PCR primers used were 5'-CC AAGCTT ATGGCAACCTCTGGAGAAGG-3' (forward) and 5'-CGC GGATCC CAGATCTTCGTCGTCAAATG-3' (reverse) (Hind III and BamHI restriction sites underlined), which were constructed based on the sequence obtained above. .. The resulting PCR products and EGFP-N1 vector (Clontech, Mountain View, CA, USA) were double digested with Hind III and BamHI restriction enzymes and ligated at 16 °C for 16 h using T4 ligase (Takara).

Activation Assay:

Article Title: Protein phosphatase 2A is requisite for the function of regulatory T cells
Article Snippet: For lentiviral overexpression of SGMS1 and SET, the full coding sequences of murine Sgms1 and Set were cloned from cDNA generated from murine CD4+ T cells using the following primers: Sgms1-cloning-for 5′-ATCTGGAATTCGCAAGCTGGGGGTACTGAAT-3′ Sgms1-cloning-rev 5′-ATTCGGGATCCCATTCCCTAGTCGGCAGAGC-3′ SET-cloning-for 5′-ATCTGGAATTCCTGTCTCCCGGTCATCTCCC-3′ SET-cloning-rev 5′-ATTCGGGATCCAGGGAGGAAAGGACTGCAAC-3′5′- They were then incorporated using EcoRI and BamHI restriction enzymes into the pLVX-EF1α-IRES-mCherry Lentiviral Vector (cat#: 631987, Clontech Laboratories) and viral particles were generated in HEK293FT cells using the ViraPower Lentiviral Packaging Mix (cat#: K4975-00, Life Technologies). .. Lentiviral infection of naïve CD4+ T cells was performed 24 h after activation with anti-CD3 plus anti-CD28 (2 μg/ml) using spin-infection during which cells were centrifuged at 900g for 90 min at 32 °C in the presence of 6 μg/ml of polybrene.

Generated:

Article Title: Protein phosphatase 2A is requisite for the function of regulatory T cells
Article Snippet: .. For lentiviral overexpression of SGMS1 and SET, the full coding sequences of murine Sgms1 and Set were cloned from cDNA generated from murine CD4+ T cells using the following primers: Sgms1-cloning-for 5′-ATCTGGAATTCGCAAGCTGGGGGTACTGAAT-3′ Sgms1-cloning-rev 5′-ATTCGGGATCCCATTCCCTAGTCGGCAGAGC-3′ SET-cloning-for 5′-ATCTGGAATTCCTGTCTCCCGGTCATCTCCC-3′ SET-cloning-rev 5′-ATTCGGGATCCAGGGAGGAAAGGACTGCAAC-3′5′- They were then incorporated using EcoRI and BamHI restriction enzymes into the pLVX-EF1α-IRES-mCherry Lentiviral Vector (cat#: 631987, Clontech Laboratories) and viral particles were generated in HEK293FT cells using the ViraPower Lentiviral Packaging Mix (cat#: K4975-00, Life Technologies). .. Viral supernatants were concentrated 100-fold with Lenti-X™ Concentrator (cat#: 631231, Clontech Laboratories).

Article Title: Mechanical Stretch Inhibits MicroRNA499 via p53 to Regulate Calcineurin-A Expression in Rat Cardiomyocytes
Article Snippet: Construction and delivery of miR499, antagomir499, and mutant-miR499 expression vector into cultured cardiomyocytes and ventricular myocardium A 85bp hsa-miR499 precursor construct as was generated as follows: (A) Genomic DNA was amplified with forward primer, CACGCCCTCTGCAGGC and reverse primer, CAGGACTCCCTCCCATGG. .. The 200bp amplified product was digested using EcoRI and BamHI restriction enzymes and ligated into pmR-ZsGreen1 plasmid vector (coexpression miR499 and green fluorescent protein, Clontech Laboratories, Mountain View, CA, USA). (B) MiR499 antagomir, and (C) mutant miR499 precursor construct was designed (Applied Biosystems) and ligated into the same plasmid vector as miR499.

Article Title: Angiotensin II Downregulates MicroRNA-145 to Regulate Kruppel-like Factor 4 and Myocardin Expression in Human Coronary Arterial Smooth Muscle Cells under High Glucose Conditions
Article Snippet: A 88bp miR-145 precursor construct was generated in the following manner. .. The 199-bp amplified product was digested with EcoRI and BamHI restriction enzymes and ligated into pmR-ZsGreen1 plasmid vector (coexpression miR-145 and green fluorescent protein) (Clontech Laboratories) digested with the same enzymes.

Amplification:

Article Title: Mechanical Stretch Inhibits MicroRNA499 via p53 to Regulate Calcineurin-A Expression in Rat Cardiomyocytes
Article Snippet: .. The 200bp amplified product was digested using EcoRI and BamHI restriction enzymes and ligated into pmR-ZsGreen1 plasmid vector (coexpression miR499 and green fluorescent protein, Clontech Laboratories, Mountain View, CA, USA). (B) MiR499 antagomir, and (C) mutant miR499 precursor construct was designed (Applied Biosystems) and ligated into the same plasmid vector as miR499. .. The constructed plasmid was transfected into cultured cardiomyoctyes or left ventricular myocardium using a low pressure-accelerated gene gun (Bioware Technologies, Taipei, Taiwan) per manufacturer instructions.

Article Title: Angiotensin II Downregulates MicroRNA-145 to Regulate Kruppel-like Factor 4 and Myocardin Expression in Human Coronary Arterial Smooth Muscle Cells under High Glucose Conditions
Article Snippet: .. The 199-bp amplified product was digested with EcoRI and BamHI restriction enzymes and ligated into pmR-ZsGreen1 plasmid vector (coexpression miR-145 and green fluorescent protein) (Clontech Laboratories) digested with the same enzymes. .. Antagomir-145 and mutant-miR-145 precursor construct was generated in pmR-ZsGreen1 plasmid vector.

Article Title: Cloning and Expression Characteristics of the Pig Stra8 Gene
Article Snippet: PCR amplification consisted of an initial 5-min denaturation step at 96 °C, followed by 35 denaturation cycles at 96 °C for 30 s, annealing at 65 °C for 30 s and extension at 72 °C for 1 min, and a final 10-min extension. .. The resulting PCR products and EGFP-N1 vector (Clontech, Mountain View, CA, USA) were double digested with Hind III and BamHI restriction enzymes and ligated at 16 °C for 16 h using T4 ligase (Takara).

cDNA Library Assay:

Article Title: Cloning and Expression Characteristics of the Pig Stra8 Gene
Article Snippet: Construction of Expression Vectors Pig Stra8 cDNA was PCR-amplified from an adult boar testicular cDNA library using the method described above and EX-Taq polymerase (Takara). .. The resulting PCR products and EGFP-N1 vector (Clontech, Mountain View, CA, USA) were double digested with Hind III and BamHI restriction enzymes and ligated at 16 °C for 16 h using T4 ligase (Takara).

Infection:

Article Title: Protein phosphatase 2A is requisite for the function of regulatory T cells
Article Snippet: For lentiviral overexpression of SGMS1 and SET, the full coding sequences of murine Sgms1 and Set were cloned from cDNA generated from murine CD4+ T cells using the following primers: Sgms1-cloning-for 5′-ATCTGGAATTCGCAAGCTGGGGGTACTGAAT-3′ Sgms1-cloning-rev 5′-ATTCGGGATCCCATTCCCTAGTCGGCAGAGC-3′ SET-cloning-for 5′-ATCTGGAATTCCTGTCTCCCGGTCATCTCCC-3′ SET-cloning-rev 5′-ATTCGGGATCCAGGGAGGAAAGGACTGCAAC-3′5′- They were then incorporated using EcoRI and BamHI restriction enzymes into the pLVX-EF1α-IRES-mCherry Lentiviral Vector (cat#: 631987, Clontech Laboratories) and viral particles were generated in HEK293FT cells using the ViraPower Lentiviral Packaging Mix (cat#: K4975-00, Life Technologies). .. Lentiviral infection of naïve CD4+ T cells was performed 24 h after activation with anti-CD3 plus anti-CD28 (2 μg/ml) using spin-infection during which cells were centrifuged at 900g for 90 min at 32 °C in the presence of 6 μg/ml of polybrene.

Expressing:

Article Title: Mechanical Stretch Inhibits MicroRNA499 via p53 to Regulate Calcineurin-A Expression in Rat Cardiomyocytes
Article Snippet: Paragraph title: Construction and delivery of miR499, antagomir499, and mutant-miR499 expression vector into cultured cardiomyocytes and ventricular myocardium ... The 200bp amplified product was digested using EcoRI and BamHI restriction enzymes and ligated into pmR-ZsGreen1 plasmid vector (coexpression miR499 and green fluorescent protein, Clontech Laboratories, Mountain View, CA, USA). (B) MiR499 antagomir, and (C) mutant miR499 precursor construct was designed (Applied Biosystems) and ligated into the same plasmid vector as miR499.

Article Title: Angiotensin II Downregulates MicroRNA-145 to Regulate Kruppel-like Factor 4 and Myocardin Expression in Human Coronary Arterial Smooth Muscle Cells under High Glucose Conditions
Article Snippet: Paragraph title: Construction and Delivery of miR-145 Expression Vector ... The 199-bp amplified product was digested with EcoRI and BamHI restriction enzymes and ligated into pmR-ZsGreen1 plasmid vector (coexpression miR-145 and green fluorescent protein) (Clontech Laboratories) digested with the same enzymes.

Article Title: Cloning and Expression Characteristics of the Pig Stra8 Gene
Article Snippet: Paragraph title: 3.8. Construction of Expression Vectors ... The resulting PCR products and EGFP-N1 vector (Clontech, Mountain View, CA, USA) were double digested with Hind III and BamHI restriction enzymes and ligated at 16 °C for 16 h using T4 ligase (Takara).

Polymerase Chain Reaction:

Article Title: Cloning and Expression Characteristics of the Pig Stra8 Gene
Article Snippet: .. The resulting PCR products and EGFP-N1 vector (Clontech, Mountain View, CA, USA) were double digested with Hind III and BamHI restriction enzymes and ligated at 16 °C for 16 h using T4 ligase (Takara). .. Expression vectors were then transformed into E. coli DH5α.

Transformation Assay:

Article Title: Cloning and Expression Characteristics of the Pig Stra8 Gene
Article Snippet: The resulting PCR products and EGFP-N1 vector (Clontech, Mountain View, CA, USA) were double digested with Hind III and BamHI restriction enzymes and ligated at 16 °C for 16 h using T4 ligase (Takara). .. Expression vectors were then transformed into E. coli DH5α.

Over Expression:

Article Title: Protein phosphatase 2A is requisite for the function of regulatory T cells
Article Snippet: .. For lentiviral overexpression of SGMS1 and SET, the full coding sequences of murine Sgms1 and Set were cloned from cDNA generated from murine CD4+ T cells using the following primers: Sgms1-cloning-for 5′-ATCTGGAATTCGCAAGCTGGGGGTACTGAAT-3′ Sgms1-cloning-rev 5′-ATTCGGGATCCCATTCCCTAGTCGGCAGAGC-3′ SET-cloning-for 5′-ATCTGGAATTCCTGTCTCCCGGTCATCTCCC-3′ SET-cloning-rev 5′-ATTCGGGATCCAGGGAGGAAAGGACTGCAAC-3′5′- They were then incorporated using EcoRI and BamHI restriction enzymes into the pLVX-EF1α-IRES-mCherry Lentiviral Vector (cat#: 631987, Clontech Laboratories) and viral particles were generated in HEK293FT cells using the ViraPower Lentiviral Packaging Mix (cat#: K4975-00, Life Technologies). .. Viral supernatants were concentrated 100-fold with Lenti-X™ Concentrator (cat#: 631231, Clontech Laboratories).

Plasmid Preparation:

Article Title: Protein phosphatase 2A is requisite for the function of regulatory T cells
Article Snippet: .. For lentiviral overexpression of SGMS1 and SET, the full coding sequences of murine Sgms1 and Set were cloned from cDNA generated from murine CD4+ T cells using the following primers: Sgms1-cloning-for 5′-ATCTGGAATTCGCAAGCTGGGGGTACTGAAT-3′ Sgms1-cloning-rev 5′-ATTCGGGATCCCATTCCCTAGTCGGCAGAGC-3′ SET-cloning-for 5′-ATCTGGAATTCCTGTCTCCCGGTCATCTCCC-3′ SET-cloning-rev 5′-ATTCGGGATCCAGGGAGGAAAGGACTGCAAC-3′5′- They were then incorporated using EcoRI and BamHI restriction enzymes into the pLVX-EF1α-IRES-mCherry Lentiviral Vector (cat#: 631987, Clontech Laboratories) and viral particles were generated in HEK293FT cells using the ViraPower Lentiviral Packaging Mix (cat#: K4975-00, Life Technologies). .. Viral supernatants were concentrated 100-fold with Lenti-X™ Concentrator (cat#: 631231, Clontech Laboratories).

Article Title: Mechanical Stretch Inhibits MicroRNA499 via p53 to Regulate Calcineurin-A Expression in Rat Cardiomyocytes
Article Snippet: .. The 200bp amplified product was digested using EcoRI and BamHI restriction enzymes and ligated into pmR-ZsGreen1 plasmid vector (coexpression miR499 and green fluorescent protein, Clontech Laboratories, Mountain View, CA, USA). (B) MiR499 antagomir, and (C) mutant miR499 precursor construct was designed (Applied Biosystems) and ligated into the same plasmid vector as miR499. .. The constructed plasmid was transfected into cultured cardiomyoctyes or left ventricular myocardium using a low pressure-accelerated gene gun (Bioware Technologies, Taipei, Taiwan) per manufacturer instructions.

Article Title: Angiotensin II Downregulates MicroRNA-145 to Regulate Kruppel-like Factor 4 and Myocardin Expression in Human Coronary Arterial Smooth Muscle Cells under High Glucose Conditions
Article Snippet: .. The 199-bp amplified product was digested with EcoRI and BamHI restriction enzymes and ligated into pmR-ZsGreen1 plasmid vector (coexpression miR-145 and green fluorescent protein) (Clontech Laboratories) digested with the same enzymes. .. Antagomir-145 and mutant-miR-145 precursor construct was generated in pmR-ZsGreen1 plasmid vector.

Article Title: Cloning and Expression Characteristics of the Pig Stra8 Gene
Article Snippet: .. The resulting PCR products and EGFP-N1 vector (Clontech, Mountain View, CA, USA) were double digested with Hind III and BamHI restriction enzymes and ligated at 16 °C for 16 h using T4 ligase (Takara). .. Expression vectors were then transformed into E. coli DH5α.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    TaKaRa restriction enzymes bamh i
    Restriction Enzymes Bamh I, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzymes bamh i/product/TaKaRa
    Average 90 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    restriction enzymes bamh i - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    Image Search Results