Structured Review

Promega bamhi restriction enzymes
Determination of copy number by Southern blot. A: EgDEF1 gene; B: gypsy retroelement. One L2T-related genotype (left: LMC343 or LMC51) is compared to one non-L2T-related genotype (right: FC2317 or FC2318). Lowercase letters signal the hybridizing bands that are predicted by the in silico digestion of the Eg133H20 BAC sequence with the appropriate restriction enzyme (a: 4,069 bp <t>EcoRI</t> fragment; b: 4,841 bp HindIII fragment; c: 6,251 <t>BamHI</t> fragment; d: 6,138 bp HindIII fragment; e: 13,132 bp BamHI fragment), asterisks indicate supernumerary bands.
Bamhi Restriction Enzymes, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bamhi restriction enzymes/product/Promega
Average 91 stars, based on 9 article reviews
Price from $9.99 to $1999.99
bamhi restriction enzymes - by Bioz Stars, 2020-08
91/100 stars

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1) Product Images from "DNA Methylation and Expression of the EgDEF1 Gene and Neighboring Retrotransposons in mantled Somaclonal Variants of Oil Palm"

Article Title: DNA Methylation and Expression of the EgDEF1 Gene and Neighboring Retrotransposons in mantled Somaclonal Variants of Oil Palm

Journal: PLoS ONE

doi: 10.1371/journal.pone.0091896

Determination of copy number by Southern blot. A: EgDEF1 gene; B: gypsy retroelement. One L2T-related genotype (left: LMC343 or LMC51) is compared to one non-L2T-related genotype (right: FC2317 or FC2318). Lowercase letters signal the hybridizing bands that are predicted by the in silico digestion of the Eg133H20 BAC sequence with the appropriate restriction enzyme (a: 4,069 bp EcoRI fragment; b: 4,841 bp HindIII fragment; c: 6,251 BamHI fragment; d: 6,138 bp HindIII fragment; e: 13,132 bp BamHI fragment), asterisks indicate supernumerary bands.
Figure Legend Snippet: Determination of copy number by Southern blot. A: EgDEF1 gene; B: gypsy retroelement. One L2T-related genotype (left: LMC343 or LMC51) is compared to one non-L2T-related genotype (right: FC2317 or FC2318). Lowercase letters signal the hybridizing bands that are predicted by the in silico digestion of the Eg133H20 BAC sequence with the appropriate restriction enzyme (a: 4,069 bp EcoRI fragment; b: 4,841 bp HindIII fragment; c: 6,251 BamHI fragment; d: 6,138 bp HindIII fragment; e: 13,132 bp BamHI fragment), asterisks indicate supernumerary bands.

Techniques Used: Southern Blot, In Silico, BAC Assay, Sequencing

Related Articles

Clone Assay:

Article Title: BRITER: A BMP Responsive Osteoblast Reporter Cell Line
Article Snippet: .. BRE-FFLuc cassette was excised from pGL3-BRE-FFLuc using NheI and BamHI restriction enzymes and cloned into pGL4.28 vector (Promega) between NheI and BamHI restriction sites to create pGL4.28-BRE-FFLuc vector. .. Internal Ribosomal Entry Site (IRES) was PCR amplified from pCAGIG vector (Addgene No. 11159) using Pfu DNA polymerase with primers AB383-Forward ( 5′-AATAGCC GCTACGTAAATTCCG -3′ ) and AB384-reverse ( 5′-T ACGCGT TAGCCGT CATATG ATATTATCATCGTGTTTT -3′ ) where nucleotides denoted in bold letters indicate IRES specific sequence and reverse primer contains MluI (single underlined) and NdeI (double underlined) restriction enzyme sites.

Article Title: A photoactivatable marker protein for pulse-chase imaging with superresolution.
Article Snippet: .. IrisFP is a photoactivatable fluorescent protein that combines irreversible photoconversion from a green- to a red-emitting form with reversible photoswitching between a fluorescent and a nonfluorescent state in both forms. .. IrisFP is a photoactivatable fluorescent protein that combines irreversible photoconversion from a green- to a red-emitting form with reversible photoswitching between a fluorescent and a nonfluorescent state in both forms.

Positron Emission Tomography:

Article Title: Structural Analysis of the DNA-binding Domain of the Helicobacter pylori Response Regulator ArsR
Article Snippet: .. Purified PCR products were digested with KpnI and BamHI restriction enzymes (Promega, Madison, WI) and ligated into linearized pET-BNK, a modified pET vector (Novagen, Madison, WI) developed specifically for expressing NMR protein targets. .. The vector contains a 5′-coding sequence for an N-terminal purification tag MRGSHHHHHHGS in-frame with the insert coding for the desired proteins.

Southern Blot:

Article Title: DNA Methylation and Expression of the EgDEF1 Gene and Neighboring Retrotransposons in mantled Somaclonal Variants of Oil Palm
Article Snippet: .. Southern blot Seven micrograms of oil palm genomic DNA extracts were digested in parallel by either EcoRI, HindIII or BamHI restriction enzymes (Promega) according to the manufacturer's instructions. .. The eletrophoretic separation of digestion products was performed as described previously before transfer on a GeneScreenPlus membrane (Perkin Elmer).

Purification:

Article Title: Nostophycin Biosynthesis Is Directed by a Hybrid Polyketide Synthase-Nonribosomal Peptide Synthetase in the Toxic Cyanobacterium Nostoc sp. Strain 152 ▿ sp. Strain 152 ▿ †
Article Snippet: .. The resulting PCR products were purified with the PCR purification kit (Edge Bio Systems), digested with XhoI and BamHI restriction enzymes (Promega), and ligated to pBluescript SK+ vector opened with the same enzymes. .. The constructed clone library was transformed to Escherichia coli DH5α.

Article Title: Structural Analysis of the DNA-binding Domain of the Helicobacter pylori Response Regulator ArsR
Article Snippet: .. Purified PCR products were digested with KpnI and BamHI restriction enzymes (Promega, Madison, WI) and ligated into linearized pET-BNK, a modified pET vector (Novagen, Madison, WI) developed specifically for expressing NMR protein targets. .. The vector contains a 5′-coding sequence for an N-terminal purification tag MRGSHHHHHHGS in-frame with the insert coding for the desired proteins.

Polymerase Chain Reaction:

Article Title: Nostophycin Biosynthesis Is Directed by a Hybrid Polyketide Synthase-Nonribosomal Peptide Synthetase in the Toxic Cyanobacterium Nostoc sp. Strain 152 ▿ sp. Strain 152 ▿ †
Article Snippet: .. The resulting PCR products were purified with the PCR purification kit (Edge Bio Systems), digested with XhoI and BamHI restriction enzymes (Promega), and ligated to pBluescript SK+ vector opened with the same enzymes. .. The constructed clone library was transformed to Escherichia coli DH5α.

Article Title: Structural Analysis of the DNA-binding Domain of the Helicobacter pylori Response Regulator ArsR
Article Snippet: .. Purified PCR products were digested with KpnI and BamHI restriction enzymes (Promega, Madison, WI) and ligated into linearized pET-BNK, a modified pET vector (Novagen, Madison, WI) developed specifically for expressing NMR protein targets. .. The vector contains a 5′-coding sequence for an N-terminal purification tag MRGSHHHHHHGS in-frame with the insert coding for the desired proteins.

Article Title: A photoactivatable marker protein for pulse-chase imaging with superresolution.
Article Snippet: .. IrisFP is a photoactivatable fluorescent protein that combines irreversible photoconversion from a green- to a red-emitting form with reversible photoswitching between a fluorescent and a nonfluorescent state in both forms. .. IrisFP is a photoactivatable fluorescent protein that combines irreversible photoconversion from a green- to a red-emitting form with reversible photoswitching between a fluorescent and a nonfluorescent state in both forms.

Nuclear Magnetic Resonance:

Article Title: Structural Analysis of the DNA-binding Domain of the Helicobacter pylori Response Regulator ArsR
Article Snippet: .. Purified PCR products were digested with KpnI and BamHI restriction enzymes (Promega, Madison, WI) and ligated into linearized pET-BNK, a modified pET vector (Novagen, Madison, WI) developed specifically for expressing NMR protein targets. .. The vector contains a 5′-coding sequence for an N-terminal purification tag MRGSHHHHHHGS in-frame with the insert coding for the desired proteins.

Amplification:

Article Title: INT6 interacts with MIF4GD/SLIP1 and is necessary for efficient histone mRNA translation
Article Snippet: .. The amplified fragments were digested with the XbaI and BamHI restriction enzymes and inserted between the XbaI and BamHI restriction sites of the pGL3 promoter (Promega). .. The promoter sequences of these histone genes were similarly amplified from Jurkat cell DNA using the following primers: H2ACP5′: 5′-GTAAAGATCTGATTTCTGCTACTTATAGGG-3′; H2ACP3′: 5′-TCCAGCCATGGCAATCAGACAAAAATCACC-3′; H2BGP5′: 5′-ATCGGTAGATCTGTGAAAGGCGCAATTTGATTGG-3′; H2BGP3′: 5′-GGTTCAGCCATGGTGTCAGAAAACAATAACAGCAG-3′; H4AP5′: 5′-GCGTGTAGATCTCATCGTCGGAACGGCGCTTCC-3′; H4AP3′: 5′-TGCCGGCCATGGCCGCTGGAGCCCGATAGACAGC-3′.

Expressing:

Article Title: Structural Analysis of the DNA-binding Domain of the Helicobacter pylori Response Regulator ArsR
Article Snippet: .. Purified PCR products were digested with KpnI and BamHI restriction enzymes (Promega, Madison, WI) and ligated into linearized pET-BNK, a modified pET vector (Novagen, Madison, WI) developed specifically for expressing NMR protein targets. .. The vector contains a 5′-coding sequence for an N-terminal purification tag MRGSHHHHHHGS in-frame with the insert coding for the desired proteins.

Modification:

Article Title: Structural Analysis of the DNA-binding Domain of the Helicobacter pylori Response Regulator ArsR
Article Snippet: .. Purified PCR products were digested with KpnI and BamHI restriction enzymes (Promega, Madison, WI) and ligated into linearized pET-BNK, a modified pET vector (Novagen, Madison, WI) developed specifically for expressing NMR protein targets. .. The vector contains a 5′-coding sequence for an N-terminal purification tag MRGSHHHHHHGS in-frame with the insert coding for the desired proteins.

Plasmid Preparation:

Article Title: Remodeling of the Inner Hair Cell Microtubule Meshwork in a Mouse Model of Auditory Neuropathy AUNA1
Article Snippet: .. The receiver plasmid was digested with XhoI and BamHI restriction enzymes (Promega). .. Subcloning was performed following the instructions from the manufacturer (Takara).

Article Title: Nostophycin Biosynthesis Is Directed by a Hybrid Polyketide Synthase-Nonribosomal Peptide Synthetase in the Toxic Cyanobacterium Nostoc sp. Strain 152 ▿ sp. Strain 152 ▿ †
Article Snippet: .. The resulting PCR products were purified with the PCR purification kit (Edge Bio Systems), digested with XhoI and BamHI restriction enzymes (Promega), and ligated to pBluescript SK+ vector opened with the same enzymes. .. The constructed clone library was transformed to Escherichia coli DH5α.

Article Title: Structural Analysis of the DNA-binding Domain of the Helicobacter pylori Response Regulator ArsR
Article Snippet: .. Purified PCR products were digested with KpnI and BamHI restriction enzymes (Promega, Madison, WI) and ligated into linearized pET-BNK, a modified pET vector (Novagen, Madison, WI) developed specifically for expressing NMR protein targets. .. The vector contains a 5′-coding sequence for an N-terminal purification tag MRGSHHHHHHGS in-frame with the insert coding for the desired proteins.

Article Title: BRITER: A BMP Responsive Osteoblast Reporter Cell Line
Article Snippet: .. BRE-FFLuc cassette was excised from pGL3-BRE-FFLuc using NheI and BamHI restriction enzymes and cloned into pGL4.28 vector (Promega) between NheI and BamHI restriction sites to create pGL4.28-BRE-FFLuc vector. .. Internal Ribosomal Entry Site (IRES) was PCR amplified from pCAGIG vector (Addgene No. 11159) using Pfu DNA polymerase with primers AB383-Forward ( 5′-AATAGCC GCTACGTAAATTCCG -3′ ) and AB384-reverse ( 5′-T ACGCGT TAGCCGT CATATG ATATTATCATCGTGTTTT -3′ ) where nucleotides denoted in bold letters indicate IRES specific sequence and reverse primer contains MluI (single underlined) and NdeI (double underlined) restriction enzyme sites.

Article Title: A photoactivatable marker protein for pulse-chase imaging with superresolution.
Article Snippet: .. IrisFP is a photoactivatable fluorescent protein that combines irreversible photoconversion from a green- to a red-emitting form with reversible photoswitching between a fluorescent and a nonfluorescent state in both forms. .. IrisFP is a photoactivatable fluorescent protein that combines irreversible photoconversion from a green- to a red-emitting form with reversible photoswitching between a fluorescent and a nonfluorescent state in both forms.

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  • 91
    Promega bamhi restriction enzymes
    Determination of copy number by Southern blot. A: EgDEF1 gene; B: gypsy retroelement. One L2T-related genotype (left: LMC343 or LMC51) is compared to one non-L2T-related genotype (right: FC2317 or FC2318). Lowercase letters signal the hybridizing bands that are predicted by the in silico digestion of the Eg133H20 BAC sequence with the appropriate restriction enzyme (a: 4,069 bp <t>EcoRI</t> fragment; b: 4,841 bp HindIII fragment; c: 6,251 <t>BamHI</t> fragment; d: 6,138 bp HindIII fragment; e: 13,132 bp BamHI fragment), asterisks indicate supernumerary bands.
    Bamhi Restriction Enzymes, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamhi restriction enzymes/product/Promega
    Average 91 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    bamhi restriction enzymes - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    85
    Promega bamh1 restriction enzyme
    PCR ( A ), Southern blot ( B ) and sequence analysis ( C ) of the selected zeocin-resistant clones. (A). Aliquots from three different clones obtained from cells that expressed wt IN (wt 1–3 ), D116A IN (D116A 1–3 ), or no IN (-IN) were subjected to total genomic DNA extraction and PCR using 5′-U3-Zeo and 3′-U5-Zeo primers (B). Total genomic DNA was digested by <t>BamH1</t> restriction enzyme and submitted to Southern blot analysis with (5′- 32 P) 5′-U3-Zeo as a probe. M: DNA size marker (bp). (C) Total genomic DNA was cut by BamH1 restriction enzyme, ligated, amplified using 5′-U3-junction and 3′-U5-junction primers and sequenced using the same primers. The name of the disrupted ORF is mentioned as well as the integration site inside the gene. The 5 bp repeats are underlined, dotted lines indicate base deletions at the integration site. ( D ) The sequences of the integrated products obtained either in an in vitro concerted integration assay [data obtained from Ref. ( 17 )] or from the in vivo yeast integration system were compared. The number of duplications and deletions in both systems are reported. 'Other duplications' indicate the number of cases that exhibited duplications comprising sizes from 3 to 6 bp.
    Bamh1 Restriction Enzyme, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamh1 restriction enzyme/product/Promega
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bamh1 restriction enzyme - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    Image Search Results


    Determination of copy number by Southern blot. A: EgDEF1 gene; B: gypsy retroelement. One L2T-related genotype (left: LMC343 or LMC51) is compared to one non-L2T-related genotype (right: FC2317 or FC2318). Lowercase letters signal the hybridizing bands that are predicted by the in silico digestion of the Eg133H20 BAC sequence with the appropriate restriction enzyme (a: 4,069 bp EcoRI fragment; b: 4,841 bp HindIII fragment; c: 6,251 BamHI fragment; d: 6,138 bp HindIII fragment; e: 13,132 bp BamHI fragment), asterisks indicate supernumerary bands.

    Journal: PLoS ONE

    Article Title: DNA Methylation and Expression of the EgDEF1 Gene and Neighboring Retrotransposons in mantled Somaclonal Variants of Oil Palm

    doi: 10.1371/journal.pone.0091896

    Figure Lengend Snippet: Determination of copy number by Southern blot. A: EgDEF1 gene; B: gypsy retroelement. One L2T-related genotype (left: LMC343 or LMC51) is compared to one non-L2T-related genotype (right: FC2317 or FC2318). Lowercase letters signal the hybridizing bands that are predicted by the in silico digestion of the Eg133H20 BAC sequence with the appropriate restriction enzyme (a: 4,069 bp EcoRI fragment; b: 4,841 bp HindIII fragment; c: 6,251 BamHI fragment; d: 6,138 bp HindIII fragment; e: 13,132 bp BamHI fragment), asterisks indicate supernumerary bands.

    Article Snippet: Southern blot Seven micrograms of oil palm genomic DNA extracts were digested in parallel by either EcoRI, HindIII or BamHI restriction enzymes (Promega) according to the manufacturer's instructions.

    Techniques: Southern Blot, In Silico, BAC Assay, Sequencing

    PCR ( A ), Southern blot ( B ) and sequence analysis ( C ) of the selected zeocin-resistant clones. (A). Aliquots from three different clones obtained from cells that expressed wt IN (wt 1–3 ), D116A IN (D116A 1–3 ), or no IN (-IN) were subjected to total genomic DNA extraction and PCR using 5′-U3-Zeo and 3′-U5-Zeo primers (B). Total genomic DNA was digested by BamH1 restriction enzyme and submitted to Southern blot analysis with (5′- 32 P) 5′-U3-Zeo as a probe. M: DNA size marker (bp). (C) Total genomic DNA was cut by BamH1 restriction enzyme, ligated, amplified using 5′-U3-junction and 3′-U5-junction primers and sequenced using the same primers. The name of the disrupted ORF is mentioned as well as the integration site inside the gene. The 5 bp repeats are underlined, dotted lines indicate base deletions at the integration site. ( D ) The sequences of the integrated products obtained either in an in vitro concerted integration assay [data obtained from Ref. ( 17 )] or from the in vivo yeast integration system were compared. The number of duplications and deletions in both systems are reported. 'Other duplications' indicate the number of cases that exhibited duplications comprising sizes from 3 to 6 bp.

    Journal: Nucleic Acids Research

    Article Title: Chromosomal integration of LTR-flanked DNA in yeast expressing HIV-1 integrase: down regulation by RAD51

    doi: 10.1093/nar/gkl843

    Figure Lengend Snippet: PCR ( A ), Southern blot ( B ) and sequence analysis ( C ) of the selected zeocin-resistant clones. (A). Aliquots from three different clones obtained from cells that expressed wt IN (wt 1–3 ), D116A IN (D116A 1–3 ), or no IN (-IN) were subjected to total genomic DNA extraction and PCR using 5′-U3-Zeo and 3′-U5-Zeo primers (B). Total genomic DNA was digested by BamH1 restriction enzyme and submitted to Southern blot analysis with (5′- 32 P) 5′-U3-Zeo as a probe. M: DNA size marker (bp). (C) Total genomic DNA was cut by BamH1 restriction enzyme, ligated, amplified using 5′-U3-junction and 3′-U5-junction primers and sequenced using the same primers. The name of the disrupted ORF is mentioned as well as the integration site inside the gene. The 5 bp repeats are underlined, dotted lines indicate base deletions at the integration site. ( D ) The sequences of the integrated products obtained either in an in vitro concerted integration assay [data obtained from Ref. ( 17 )] or from the in vivo yeast integration system were compared. The number of duplications and deletions in both systems are reported. 'Other duplications' indicate the number of cases that exhibited duplications comprising sizes from 3 to 6 bp.

    Article Snippet: Southern blot analysis was performed on genomic DNA according to a previously described protocol ( ) and using BamH1 restriction enzyme (PROMEGA) and the 32 P-labeled ODN, 5′-32 P-U3-Zeo described above as probe.

    Techniques: Polymerase Chain Reaction, Southern Blot, Sequencing, Clone Assay, DNA Extraction, Marker, Amplification, In Vitro, In Vivo