Journal: PLoS ONE

Article Title: Post-Injury Treatment with 7,8-Dihydroxyflavone, a TrkB Receptor Agonist, Protects against Experimental Traumatic Brain Injury via PI3K/Akt Signaling

doi: 10.1371/journal.pone.0113397

Figure Lengend Snippet: Antibodies used in immunofluorescence and western blot.

Article Snippet: Phospho-Bad Ser136 , Cell signaling , 4366 , Rabbit , Monoclonal , WB 1∶1000.

Techniques: Immunofluorescence, Western Blot

Journal: PLoS ONE

Article Title: Salidroside Improves Behavioral and Histological Outcomes and Reduces Apoptosis via PI3K/Akt Signaling after Experimental Traumatic Brain Injury

doi: 10.1371/journal.pone.0045763

Figure Lengend Snippet: ( A, B, C ) Representative immunoblots and densitometric analysis showed a significant increase in p-Akt Ser473 level in the ipsilateral cortex of 20 mg/kg salidroside (SALD 20)-treated injured mice at day 1 compared with vehicle-treated injured mice. The level of p-Akt Thr308 was increased following SALD 20 treatment at day 3 compared with vehicle-treated injured mice. ( D, E, F ) Representative immunoblots and densitometric analysis showed that there were no differences in the levels of p-Bad and p-FOXO1 in the ipsilateral cortex between the SALD 20 and vehicle groups at day 1 or 3. ( G, H, I ) Representative immunoblots and densitometric analysis showed that the mitochondrial Bcl-2/Bax ratio significantly increased in SALD-20 treated injured mice but no difference was found in the total Bcl-2/Bax ratio. Values are presented as mean ± SEM; # P <0.05, ## P <0.01 versus sham controls, and * P <0.05 versus vehicle-treated injured mice (n = 6 mice/group at each time point, repeated measures two-way ANOVA for p-Akt Ser473, p-Akt Thr308, p-Bad, and p-FOXO1 levels; one-way ANOVA for Bcl-2/Bax ratio).

Article Snippet: p-Bad (Ser136) , Cell signaling , 4366 , Rabbit , Monoclonal , WB 1∶1000.

Techniques: Western Blot

Journal: PLoS ONE

Article Title: Salidroside Improves Behavioral and Histological Outcomes and Reduces Apoptosis via PI3K/Akt Signaling after Experimental Traumatic Brain Injury

doi: 10.1371/journal.pone.0045763

Figure Lengend Snippet: Representative immunoblots and densitometric analysis showed that (A, B, C, D) levels of cleaved caspase 3, p-Akt Ser473, p-Akt Thr308, (E, F, G) p-Bad and p-FOXO1 in ipsilateral hippocampal samples from injured animals at post-injury day 1 or 3 were not significantly different from those in sham-injured animals (n = 6 mice/group at each time point, repeated measures two-way ANOVA).

Article Snippet: p-Bad (Ser136) , Cell signaling , 4366 , Rabbit , Monoclonal , WB 1∶1000.

Techniques: Western Blot

Journal: PLoS ONE

Article Title: Salidroside Improves Behavioral and Histological Outcomes and Reduces Apoptosis via PI3K/Akt Signaling after Experimental Traumatic Brain Injury

doi: 10.1371/journal.pone.0045763

Figure Lengend Snippet: ( A, B, C, D, E, F ) Representative immunoblots and densitometric analysis in the cortex from sham-injured mice or mice subject to cortical impact injury, infused intracerebroventricular (icv) with saline (vehicle) or LY294002. Mice received either icv pretreatment of LY294002 followed by 20 mg/kg salidroside (SALD 20) after injury (S20Y group) or icv pretreatment of saline followed by SALD 20 after CCI (S20V group). LY294002 did not affect Akt, Bad, or FOXO1 phosphorylation in sham-operated mice, but it significantly abolished SALD 20-induced preservation of p-Akt Thr308 and p-FOXO1 levels in the ipsilateral hemisphere at 1 day post-injury. The levels of p-Akt Ser473 and p-Bad were similar between the S20V and S20LY groups. Values are presented as mean ± SEM; * P <0.05, ** P <0.01 versus sham + vehicle (ShamV), and & P <0.05 versus sham + LY294002 (ShamLY), and # P <0.05 versus S20V group (n = 6 mice/group, one-way ANOVA). ( G ) Representative cresyl violet-stained brain sections of S20V and S20LY mice at 28 days post-injury. Scale bar is 1 mm. ( H ) Quantification analysis showed that there was a significant decrease in the residual cerebral tissue ratio in the S20LY group compared with the S20S group. Values are presented as mean ± SEM; * P <0.05 versus S20V group (n = 6 mice/group, Student’s t -test).

Article Snippet: p-Bad (Ser136) , Cell signaling , 4366 , Rabbit , Monoclonal , WB 1∶1000.

Techniques: Western Blot, Preserving, Staining

Journal: PLoS ONE

Article Title: Salidroside Improves Behavioral and Histological Outcomes and Reduces Apoptosis via PI3K/Akt Signaling after Experimental Traumatic Brain Injury

doi: 10.1371/journal.pone.0045763

Figure Lengend Snippet: Antibodies used in immunofluorescence and western blot.

Article Snippet: p-Bad (Ser136) , Cell signaling , 4366 , Rabbit , Monoclonal , WB 1∶1000.

Techniques: Immunofluorescence, Western Blot

Journal: PLoS ONE

Article Title: c-MET Protects Breast Cancer Cells from Apoptosis Induced by Sodium Butyrate

doi: 10.1371/journal.pone.0030143

Figure Lengend Snippet: (A) to (D) showed the effect of NaBu on breast cancer cell line MDA-MB-231(A and B) and MCF-7 (C and D). Majority of MDA-MB-231(B) and MCF-7(D) cancer cells were removed by the treatment of NaBu. Compared with 16% of viable MCF cells, the survival rate of MDA-MB-231 was significantly increased to 30%(E). Immunoblotting result of BAD, BAX, Bcl-2 and Cytochrome C expression indicated that MDA-MB-231 cells were resistant to the treatment of NaBu by neutralizing its apoptosis effect compared with MCF-7 cells (F). *P<0.05.

Article Snippet: After blocking in a powdered nonfat milk solution (5% in PBS) with 0.05% Tween-20, the blot was incubated with a polyclonal rabbit anti- human Bcl-2-associated death promoter (BAD) antibody (Cell Signaling, Danvers, MA),a rabbit anti- human BAX antibody (Cell Signaling), a rabbit anti- human Bcl-2 antibody (Cell Signaling), a rabbit anti- human Cytochrome C antibody (Cell Signaling), a rabbit anti-human CD133 antibody (Cell Signaling) and a rabbit anti-human beta– actin antibody (Cell Signaling) at 1∶1000 dilutions in 5% blocking solution over night at 4°C.

Techniques: Western Blot, Expressing

Journal: PLoS ONE

Article Title: Immunohistochemistry of Colorectal Cancer Biomarker Phosphorylation Requires Controlled Tissue Fixation

doi: 10.1371/journal.pone.0113608

Figure Lengend Snippet: All antibodies used for detection in this study.

Article Snippet: Phospho-BAD , 40A9 , 1∶40 , Ser112 , Cell Signalling Technologies, Danvers, MA , 5284.

Techniques: