bacteroides thetaiotaomicron atcc 29148  (ATCC)


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    ATCC bacteroides thetaiotaomicron atcc 29148
    Bacteroides Thetaiotaomicron Atcc 29148, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nctc 9343 johnson41 nctc 9343 bacteroides thetaiotaomicron vpi 5482 atcc  (ATCC)


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    ATCC nctc 9343 johnson41 nctc 9343 bacteroides thetaiotaomicron vpi 5482 atcc
    Nctc 9343 Johnson41 Nctc 9343 Bacteroides Thetaiotaomicron Vpi 5482 Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bacteroides thetaiotaomicron vpi 5482  (ATCC)


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    ATCC bacteroides thetaiotaomicron vpi 5482
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    bacteroides thetaiotaomicron atcc 29741  (ATCC)


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    ATCC bacteroides thetaiotaomicron atcc 29741
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    bacteroides thetaiotaomicron  (ATCC)


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    ATCC bacteroides thetaiotaomicron
    <t>B.Theta</t> abundance is positively correlated with the improvement of lipid metabolism dysfunction in NAFLD mice. (a) Schematic of the experimental design. (b, c) triglyceride (TG) (b) and cholesterol (CHO)(c) in the mouse liver ( n = 6 ~ 7). (d) Representative liver histology visualized by H&E staining, oil red O (ORO) staining, and Masson’s trichrome staining. (e,f) NAFLD activity score (NAS) (e) and fibrosis score (f) of mouse liver ( n = 6 ~ 7). (g-h) the abundance of the most prevalent gut microbiota at the family (g) and genus (h) levels analyzed by 16S rRNA gene sequencing with fecal samples ( n = 5). (i) B. theta abundance at the species level ( n = 5). (j) Predicted lipid metabolism-related microbiome function based on the KEGG database. (k) B. theta abundance at the species level in mice with WD/CCl 4 treatment for 12 weeks. Data were presented as means with SD or represented figures. * p < .05 and ** p < .01 vs . model or control group. ns, not significant. N, Control; M, WD/CCl 4 /DEN; B, WD/CCl 4 /DEN+BBR (200 mg/kg).
    Bacteroides Thetaiotaomicron, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Bacteroides thetaiotaomicron ameliorates mouse hepatic steatosis through regulating gut microbial composition, gut-liver folate and unsaturated fatty acids metabolism"

    Article Title: Bacteroides thetaiotaomicron ameliorates mouse hepatic steatosis through regulating gut microbial composition, gut-liver folate and unsaturated fatty acids metabolism

    Journal: Gut Microbes

    doi: 10.1080/19490976.2024.2304159

    B.Theta abundance is positively correlated with the improvement of lipid metabolism dysfunction in NAFLD mice. (a) Schematic of the experimental design. (b, c) triglyceride (TG) (b) and cholesterol (CHO)(c) in the mouse liver ( n = 6 ~ 7). (d) Representative liver histology visualized by H&E staining, oil red O (ORO) staining, and Masson’s trichrome staining. (e,f) NAFLD activity score (NAS) (e) and fibrosis score (f) of mouse liver ( n = 6 ~ 7). (g-h) the abundance of the most prevalent gut microbiota at the family (g) and genus (h) levels analyzed by 16S rRNA gene sequencing with fecal samples ( n = 5). (i) B. theta abundance at the species level ( n = 5). (j) Predicted lipid metabolism-related microbiome function based on the KEGG database. (k) B. theta abundance at the species level in mice with WD/CCl 4 treatment for 12 weeks. Data were presented as means with SD or represented figures. * p < .05 and ** p < .01 vs . model or control group. ns, not significant. N, Control; M, WD/CCl 4 /DEN; B, WD/CCl 4 /DEN+BBR (200 mg/kg).
    Figure Legend Snippet: B.Theta abundance is positively correlated with the improvement of lipid metabolism dysfunction in NAFLD mice. (a) Schematic of the experimental design. (b, c) triglyceride (TG) (b) and cholesterol (CHO)(c) in the mouse liver ( n = 6 ~ 7). (d) Representative liver histology visualized by H&E staining, oil red O (ORO) staining, and Masson’s trichrome staining. (e,f) NAFLD activity score (NAS) (e) and fibrosis score (f) of mouse liver ( n = 6 ~ 7). (g-h) the abundance of the most prevalent gut microbiota at the family (g) and genus (h) levels analyzed by 16S rRNA gene sequencing with fecal samples ( n = 5). (i) B. theta abundance at the species level ( n = 5). (j) Predicted lipid metabolism-related microbiome function based on the KEGG database. (k) B. theta abundance at the species level in mice with WD/CCl 4 treatment for 12 weeks. Data were presented as means with SD or represented figures. * p < .05 and ** p < .01 vs . model or control group. ns, not significant. N, Control; M, WD/CCl 4 /DEN; B, WD/CCl 4 /DEN+BBR (200 mg/kg).

    Techniques Used: Staining, Activity Assay, Sequencing

    B. theta alleviates hyperlipidemia and insulin resistance in NAFLD mice. (a) Animal experimental schedule. (b) Body weight. (c) Body fat. (d) Average food intake per week. (e) Serum TG. (f) Serum CHO. (g) Non-fasting blood glucose. (h) Fasting insulin. (i) HOMA2-IR index. Data are presented as means with SD. n = 6, * p < .05 and ** p < .01 vs . model or control group. ns, not significant.
    Figure Legend Snippet: B. theta alleviates hyperlipidemia and insulin resistance in NAFLD mice. (a) Animal experimental schedule. (b) Body weight. (c) Body fat. (d) Average food intake per week. (e) Serum TG. (f) Serum CHO. (g) Non-fasting blood glucose. (h) Fasting insulin. (i) HOMA2-IR index. Data are presented as means with SD. n = 6, * p < .05 and ** p < .01 vs . model or control group. ns, not significant.

    Techniques Used:

    B. theta treatment prevents hepatic steatohepatitis and liver injury in NAFLD mice. (a) Serum ALT. (b) Serum AST. (c) Representative liver appearance, H&E staining, oil red O (ORO) staining, and F4/80 immunohistochemical stains of liver sections. (d) Liver weight. (e) Liver index; (f) quantification of NAFLD activity score (NAS), including steatosis, ballooning, and inflammation grade. (g) Liver TG. (h) Liver CHO. The data are presented as means with SD; n = 6, * p < .05 and ** p < .01 vs . model or control group. ns, not significant.
    Figure Legend Snippet: B. theta treatment prevents hepatic steatohepatitis and liver injury in NAFLD mice. (a) Serum ALT. (b) Serum AST. (c) Representative liver appearance, H&E staining, oil red O (ORO) staining, and F4/80 immunohistochemical stains of liver sections. (d) Liver weight. (e) Liver index; (f) quantification of NAFLD activity score (NAS), including steatosis, ballooning, and inflammation grade. (g) Liver TG. (h) Liver CHO. The data are presented as means with SD; n = 6, * p < .05 and ** p < .01 vs . model or control group. ns, not significant.

    Techniques Used: Staining, Immunohistochemistry, Activity Assay

    B. theta treatment modulates the gut microbial composition in NAFLD mice. The gut microbial composition was obtained through 16s-RNA-amplicon sequencing. (a) Plots of operational taxonomic units (OTUs). (b) Changes in microbiota diversity presented by the Simpson’s index of diversity and Shannon index. (c) Relative abundance of bacteria at the phylum level. (d) The ratio of firmicutes to Bacteroidetes. (e) Prediction of lipid metabolism-related microbiome function based on KEGG database. The data are presented as means with SD; n = 6, * p < .05 and ** p < .01 vs . model or control group. ns, not significant.
    Figure Legend Snippet: B. theta treatment modulates the gut microbial composition in NAFLD mice. The gut microbial composition was obtained through 16s-RNA-amplicon sequencing. (a) Plots of operational taxonomic units (OTUs). (b) Changes in microbiota diversity presented by the Simpson’s index of diversity and Shannon index. (c) Relative abundance of bacteria at the phylum level. (d) The ratio of firmicutes to Bacteroidetes. (e) Prediction of lipid metabolism-related microbiome function based on KEGG database. The data are presented as means with SD; n = 6, * p < .05 and ** p < .01 vs . model or control group. ns, not significant.

    Techniques Used: Amplification, Sequencing, Bacteria

    B. theta treatment enhances gut folate biosynthesis and modulates hepatic folate metabolism in NAFLD mice. (a) Schematic illustrates a network of folate-mediated one-carbon metabolism. (b,c) total folate level in mouse fecal (b) and liver (c). (d-f) SAM (d), GSH (e), and PC (f) in the mouse liver. (g) mRNAs related to folate-mediated one-carbon metabolism. The data are presented as means with SD; n = 6, * p < .05 and ** p < .01 vs . model or control group. ns, not significant. GSH, glutathione; met, methionine; SAM, S-adenosylmethionine; SAH, S-adenosylhomocysteine; HCY, homocysteine; PE, phosphatidylethanolamine; PC, phosphatidylcholine; MS, vitamin B12-dependent methionine synthase; 5-MTHF, 5-methyltetrahydrofolate.
    Figure Legend Snippet: B. theta treatment enhances gut folate biosynthesis and modulates hepatic folate metabolism in NAFLD mice. (a) Schematic illustrates a network of folate-mediated one-carbon metabolism. (b,c) total folate level in mouse fecal (b) and liver (c). (d-f) SAM (d), GSH (e), and PC (f) in the mouse liver. (g) mRNAs related to folate-mediated one-carbon metabolism. The data are presented as means with SD; n = 6, * p < .05 and ** p < .01 vs . model or control group. ns, not significant. GSH, glutathione; met, methionine; SAM, S-adenosylmethionine; SAH, S-adenosylhomocysteine; HCY, homocysteine; PE, phosphatidylethanolamine; PC, phosphatidylcholine; MS, vitamin B12-dependent methionine synthase; 5-MTHF, 5-methyltetrahydrofolate.

    Techniques Used:

    B. theta regulates the unsaturated fatty acid in NAFLD mice. (a) Fatty acid composition and the elongation and desaturation process. (b-e) percent composition of total SFAs (b), total MUFAs (c), C16:1 fatty acid (d), and total PUFAs (e). (f) PUFA/MUFA ratio. (g,h) the contents of C18:2, C18:3, C18:4, C20:4, C20:5, and C22:6 PUFAs; (i-k) desaturase activity of stearoyl-CoA desaturase 1 (SCD1), fatty acid desaturase 1 (FADS1) and FADS2. The data are presented as means with SD; n = 6, * p < .05 and ** p < .01 vs . model or control group. ns, not significant.
    Figure Legend Snippet: B. theta regulates the unsaturated fatty acid in NAFLD mice. (a) Fatty acid composition and the elongation and desaturation process. (b-e) percent composition of total SFAs (b), total MUFAs (c), C16:1 fatty acid (d), and total PUFAs (e). (f) PUFA/MUFA ratio. (g,h) the contents of C18:2, C18:3, C18:4, C20:4, C20:5, and C22:6 PUFAs; (i-k) desaturase activity of stearoyl-CoA desaturase 1 (SCD1), fatty acid desaturase 1 (FADS1) and FADS2. The data are presented as means with SD; n = 6, * p < .05 and ** p < .01 vs . model or control group. ns, not significant.

    Techniques Used: Activity Assay

    Bacteroides thetaiotaomicron ( B. theta ) administration alleviates nonalcoholic fatty liver disease (NAFLD) in HFD-induced mice through multiple mechanisms. A high-fat diet-induced fatty liver in mice accompanied by dysbiosis of gut microbiota and its metabolites. B. theta administration resulted in several beneficial effects, as indicated by the following changes: 1) increase in microbiota diversity and decrease in Firmicutes/Bacteroidetes (F/B) ratio; 2) enhancement of gut folate biosynthesis and hepatic folate metabolism; 3) increase in hepatic polyunsaturated fatty acids (PUFAs) and decrease in monounsaturated fatty acids (MUFAs) levels, with decreased PUFA/MUFA ratio. These multiple mechanisms collectively contribute to the therapeutic effects of B. theta on the development of NAFLD.
    Figure Legend Snippet: Bacteroides thetaiotaomicron ( B. theta ) administration alleviates nonalcoholic fatty liver disease (NAFLD) in HFD-induced mice through multiple mechanisms. A high-fat diet-induced fatty liver in mice accompanied by dysbiosis of gut microbiota and its metabolites. B. theta administration resulted in several beneficial effects, as indicated by the following changes: 1) increase in microbiota diversity and decrease in Firmicutes/Bacteroidetes (F/B) ratio; 2) enhancement of gut folate biosynthesis and hepatic folate metabolism; 3) increase in hepatic polyunsaturated fatty acids (PUFAs) and decrease in monounsaturated fatty acids (MUFAs) levels, with decreased PUFA/MUFA ratio. These multiple mechanisms collectively contribute to the therapeutic effects of B. theta on the development of NAFLD.

    Techniques Used:

    bacteroides thetaiotaomicron atcc 29741  (ATCC)


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    ATCC bacteroides thetaiotaomicron atcc 29741
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    bacteroides thetaiotaomicron vpi 5482  (ATCC)


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    ATCC bacteroides thetaiotaomicron vpi 5482
    Bacterial strains used
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    1) Product Images from "Hadza Prevotella require diet-derived microbiota-accessible carbohydrates to persist in mice"

    Article Title: Hadza Prevotella require diet-derived microbiota-accessible carbohydrates to persist in mice

    Journal: Cell reports

    doi: 10.1016/j.celrep.2023.113233

    Bacterial strains used
    Figure Legend Snippet: Bacterial strains used

    Techniques Used:


    Figure Legend Snippet:

    Techniques Used: DNA Purification, Purification, SYBR Green Assay, Real-time Polymerase Chain Reaction, Isolation, Bacteria, Sequencing, Software, Western Blot

    blood bacteroides thetaiotaomicron vpi 5482  (ATCC)


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    ATCC blood bacteroides thetaiotaomicron vpi 5482
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    bacteroides thetaiotaomicron vpi 5482  (ATCC)


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    bacteroides thetaiotaomicron  (ATCC)


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    ATCC bacteroides thetaiotaomicron
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    ATCC bacteroides thetaiotaomicron atcc 29148
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    ATCC nctc 9343 johnson41 nctc 9343 bacteroides thetaiotaomicron vpi 5482 atcc
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    ATCC bacteroides thetaiotaomicron vpi 5482
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    ATCC bacteroides thetaiotaomicron atcc 29741
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    ATCC bacteroides thetaiotaomicron
    <t>B.Theta</t> abundance is positively correlated with the improvement of lipid metabolism dysfunction in NAFLD mice. (a) Schematic of the experimental design. (b, c) triglyceride (TG) (b) and cholesterol (CHO)(c) in the mouse liver ( n = 6 ~ 7). (d) Representative liver histology visualized by H&E staining, oil red O (ORO) staining, and Masson’s trichrome staining. (e,f) NAFLD activity score (NAS) (e) and fibrosis score (f) of mouse liver ( n = 6 ~ 7). (g-h) the abundance of the most prevalent gut microbiota at the family (g) and genus (h) levels analyzed by 16S rRNA gene sequencing with fecal samples ( n = 5). (i) B. theta abundance at the species level ( n = 5). (j) Predicted lipid metabolism-related microbiome function based on the KEGG database. (k) B. theta abundance at the species level in mice with WD/CCl 4 treatment for 12 weeks. Data were presented as means with SD or represented figures. * p < .05 and ** p < .01 vs . model or control group. ns, not significant. N, Control; M, WD/CCl 4 /DEN; B, WD/CCl 4 /DEN+BBR (200 mg/kg).
    Bacteroides Thetaiotaomicron, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC blood bacteroides thetaiotaomicron vpi 5482
    <t>B.Theta</t> abundance is positively correlated with the improvement of lipid metabolism dysfunction in NAFLD mice. (a) Schematic of the experimental design. (b, c) triglyceride (TG) (b) and cholesterol (CHO)(c) in the mouse liver ( n = 6 ~ 7). (d) Representative liver histology visualized by H&E staining, oil red O (ORO) staining, and Masson’s trichrome staining. (e,f) NAFLD activity score (NAS) (e) and fibrosis score (f) of mouse liver ( n = 6 ~ 7). (g-h) the abundance of the most prevalent gut microbiota at the family (g) and genus (h) levels analyzed by 16S rRNA gene sequencing with fecal samples ( n = 5). (i) B. theta abundance at the species level ( n = 5). (j) Predicted lipid metabolism-related microbiome function based on the KEGG database. (k) B. theta abundance at the species level in mice with WD/CCl 4 treatment for 12 weeks. Data were presented as means with SD or represented figures. * p < .05 and ** p < .01 vs . model or control group. ns, not significant. N, Control; M, WD/CCl 4 /DEN; B, WD/CCl 4 /DEN+BBR (200 mg/kg).
    Blood Bacteroides Thetaiotaomicron Vpi 5482, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    B.Theta abundance is positively correlated with the improvement of lipid metabolism dysfunction in NAFLD mice. (a) Schematic of the experimental design. (b, c) triglyceride (TG) (b) and cholesterol (CHO)(c) in the mouse liver ( n = 6 ~ 7). (d) Representative liver histology visualized by H&E staining, oil red O (ORO) staining, and Masson’s trichrome staining. (e,f) NAFLD activity score (NAS) (e) and fibrosis score (f) of mouse liver ( n = 6 ~ 7). (g-h) the abundance of the most prevalent gut microbiota at the family (g) and genus (h) levels analyzed by 16S rRNA gene sequencing with fecal samples ( n = 5). (i) B. theta abundance at the species level ( n = 5). (j) Predicted lipid metabolism-related microbiome function based on the KEGG database. (k) B. theta abundance at the species level in mice with WD/CCl 4 treatment for 12 weeks. Data were presented as means with SD or represented figures. * p < .05 and ** p < .01 vs . model or control group. ns, not significant. N, Control; M, WD/CCl 4 /DEN; B, WD/CCl 4 /DEN+BBR (200 mg/kg).

    Journal: Gut Microbes

    Article Title: Bacteroides thetaiotaomicron ameliorates mouse hepatic steatosis through regulating gut microbial composition, gut-liver folate and unsaturated fatty acids metabolism

    doi: 10.1080/19490976.2024.2304159

    Figure Lengend Snippet: B.Theta abundance is positively correlated with the improvement of lipid metabolism dysfunction in NAFLD mice. (a) Schematic of the experimental design. (b, c) triglyceride (TG) (b) and cholesterol (CHO)(c) in the mouse liver ( n = 6 ~ 7). (d) Representative liver histology visualized by H&E staining, oil red O (ORO) staining, and Masson’s trichrome staining. (e,f) NAFLD activity score (NAS) (e) and fibrosis score (f) of mouse liver ( n = 6 ~ 7). (g-h) the abundance of the most prevalent gut microbiota at the family (g) and genus (h) levels analyzed by 16S rRNA gene sequencing with fecal samples ( n = 5). (i) B. theta abundance at the species level ( n = 5). (j) Predicted lipid metabolism-related microbiome function based on the KEGG database. (k) B. theta abundance at the species level in mice with WD/CCl 4 treatment for 12 weeks. Data were presented as means with SD or represented figures. * p < .05 and ** p < .01 vs . model or control group. ns, not significant. N, Control; M, WD/CCl 4 /DEN; B, WD/CCl 4 /DEN+BBR (200 mg/kg).

    Article Snippet: The Bacteroides thetaiotaomicron ( B. theta ) strain ATCC 29,148 was derived from ATCC and cultured on blood agar at 37°C under strictly anaerobic conditions.

    Techniques: Staining, Activity Assay, Sequencing

    B. theta alleviates hyperlipidemia and insulin resistance in NAFLD mice. (a) Animal experimental schedule. (b) Body weight. (c) Body fat. (d) Average food intake per week. (e) Serum TG. (f) Serum CHO. (g) Non-fasting blood glucose. (h) Fasting insulin. (i) HOMA2-IR index. Data are presented as means with SD. n = 6, * p < .05 and ** p < .01 vs . model or control group. ns, not significant.

    Journal: Gut Microbes

    Article Title: Bacteroides thetaiotaomicron ameliorates mouse hepatic steatosis through regulating gut microbial composition, gut-liver folate and unsaturated fatty acids metabolism

    doi: 10.1080/19490976.2024.2304159

    Figure Lengend Snippet: B. theta alleviates hyperlipidemia and insulin resistance in NAFLD mice. (a) Animal experimental schedule. (b) Body weight. (c) Body fat. (d) Average food intake per week. (e) Serum TG. (f) Serum CHO. (g) Non-fasting blood glucose. (h) Fasting insulin. (i) HOMA2-IR index. Data are presented as means with SD. n = 6, * p < .05 and ** p < .01 vs . model or control group. ns, not significant.

    Article Snippet: The Bacteroides thetaiotaomicron ( B. theta ) strain ATCC 29,148 was derived from ATCC and cultured on blood agar at 37°C under strictly anaerobic conditions.

    Techniques:

    B. theta treatment prevents hepatic steatohepatitis and liver injury in NAFLD mice. (a) Serum ALT. (b) Serum AST. (c) Representative liver appearance, H&E staining, oil red O (ORO) staining, and F4/80 immunohistochemical stains of liver sections. (d) Liver weight. (e) Liver index; (f) quantification of NAFLD activity score (NAS), including steatosis, ballooning, and inflammation grade. (g) Liver TG. (h) Liver CHO. The data are presented as means with SD; n = 6, * p < .05 and ** p < .01 vs . model or control group. ns, not significant.

    Journal: Gut Microbes

    Article Title: Bacteroides thetaiotaomicron ameliorates mouse hepatic steatosis through regulating gut microbial composition, gut-liver folate and unsaturated fatty acids metabolism

    doi: 10.1080/19490976.2024.2304159

    Figure Lengend Snippet: B. theta treatment prevents hepatic steatohepatitis and liver injury in NAFLD mice. (a) Serum ALT. (b) Serum AST. (c) Representative liver appearance, H&E staining, oil red O (ORO) staining, and F4/80 immunohistochemical stains of liver sections. (d) Liver weight. (e) Liver index; (f) quantification of NAFLD activity score (NAS), including steatosis, ballooning, and inflammation grade. (g) Liver TG. (h) Liver CHO. The data are presented as means with SD; n = 6, * p < .05 and ** p < .01 vs . model or control group. ns, not significant.

    Article Snippet: The Bacteroides thetaiotaomicron ( B. theta ) strain ATCC 29,148 was derived from ATCC and cultured on blood agar at 37°C under strictly anaerobic conditions.

    Techniques: Staining, Immunohistochemistry, Activity Assay

    B. theta treatment modulates the gut microbial composition in NAFLD mice. The gut microbial composition was obtained through 16s-RNA-amplicon sequencing. (a) Plots of operational taxonomic units (OTUs). (b) Changes in microbiota diversity presented by the Simpson’s index of diversity and Shannon index. (c) Relative abundance of bacteria at the phylum level. (d) The ratio of firmicutes to Bacteroidetes. (e) Prediction of lipid metabolism-related microbiome function based on KEGG database. The data are presented as means with SD; n = 6, * p < .05 and ** p < .01 vs . model or control group. ns, not significant.

    Journal: Gut Microbes

    Article Title: Bacteroides thetaiotaomicron ameliorates mouse hepatic steatosis through regulating gut microbial composition, gut-liver folate and unsaturated fatty acids metabolism

    doi: 10.1080/19490976.2024.2304159

    Figure Lengend Snippet: B. theta treatment modulates the gut microbial composition in NAFLD mice. The gut microbial composition was obtained through 16s-RNA-amplicon sequencing. (a) Plots of operational taxonomic units (OTUs). (b) Changes in microbiota diversity presented by the Simpson’s index of diversity and Shannon index. (c) Relative abundance of bacteria at the phylum level. (d) The ratio of firmicutes to Bacteroidetes. (e) Prediction of lipid metabolism-related microbiome function based on KEGG database. The data are presented as means with SD; n = 6, * p < .05 and ** p < .01 vs . model or control group. ns, not significant.

    Article Snippet: The Bacteroides thetaiotaomicron ( B. theta ) strain ATCC 29,148 was derived from ATCC and cultured on blood agar at 37°C under strictly anaerobic conditions.

    Techniques: Amplification, Sequencing, Bacteria

    B. theta treatment enhances gut folate biosynthesis and modulates hepatic folate metabolism in NAFLD mice. (a) Schematic illustrates a network of folate-mediated one-carbon metabolism. (b,c) total folate level in mouse fecal (b) and liver (c). (d-f) SAM (d), GSH (e), and PC (f) in the mouse liver. (g) mRNAs related to folate-mediated one-carbon metabolism. The data are presented as means with SD; n = 6, * p < .05 and ** p < .01 vs . model or control group. ns, not significant. GSH, glutathione; met, methionine; SAM, S-adenosylmethionine; SAH, S-adenosylhomocysteine; HCY, homocysteine; PE, phosphatidylethanolamine; PC, phosphatidylcholine; MS, vitamin B12-dependent methionine synthase; 5-MTHF, 5-methyltetrahydrofolate.

    Journal: Gut Microbes

    Article Title: Bacteroides thetaiotaomicron ameliorates mouse hepatic steatosis through regulating gut microbial composition, gut-liver folate and unsaturated fatty acids metabolism

    doi: 10.1080/19490976.2024.2304159

    Figure Lengend Snippet: B. theta treatment enhances gut folate biosynthesis and modulates hepatic folate metabolism in NAFLD mice. (a) Schematic illustrates a network of folate-mediated one-carbon metabolism. (b,c) total folate level in mouse fecal (b) and liver (c). (d-f) SAM (d), GSH (e), and PC (f) in the mouse liver. (g) mRNAs related to folate-mediated one-carbon metabolism. The data are presented as means with SD; n = 6, * p < .05 and ** p < .01 vs . model or control group. ns, not significant. GSH, glutathione; met, methionine; SAM, S-adenosylmethionine; SAH, S-adenosylhomocysteine; HCY, homocysteine; PE, phosphatidylethanolamine; PC, phosphatidylcholine; MS, vitamin B12-dependent methionine synthase; 5-MTHF, 5-methyltetrahydrofolate.

    Article Snippet: The Bacteroides thetaiotaomicron ( B. theta ) strain ATCC 29,148 was derived from ATCC and cultured on blood agar at 37°C under strictly anaerobic conditions.

    Techniques:

    B. theta regulates the unsaturated fatty acid in NAFLD mice. (a) Fatty acid composition and the elongation and desaturation process. (b-e) percent composition of total SFAs (b), total MUFAs (c), C16:1 fatty acid (d), and total PUFAs (e). (f) PUFA/MUFA ratio. (g,h) the contents of C18:2, C18:3, C18:4, C20:4, C20:5, and C22:6 PUFAs; (i-k) desaturase activity of stearoyl-CoA desaturase 1 (SCD1), fatty acid desaturase 1 (FADS1) and FADS2. The data are presented as means with SD; n = 6, * p < .05 and ** p < .01 vs . model or control group. ns, not significant.

    Journal: Gut Microbes

    Article Title: Bacteroides thetaiotaomicron ameliorates mouse hepatic steatosis through regulating gut microbial composition, gut-liver folate and unsaturated fatty acids metabolism

    doi: 10.1080/19490976.2024.2304159

    Figure Lengend Snippet: B. theta regulates the unsaturated fatty acid in NAFLD mice. (a) Fatty acid composition and the elongation and desaturation process. (b-e) percent composition of total SFAs (b), total MUFAs (c), C16:1 fatty acid (d), and total PUFAs (e). (f) PUFA/MUFA ratio. (g,h) the contents of C18:2, C18:3, C18:4, C20:4, C20:5, and C22:6 PUFAs; (i-k) desaturase activity of stearoyl-CoA desaturase 1 (SCD1), fatty acid desaturase 1 (FADS1) and FADS2. The data are presented as means with SD; n = 6, * p < .05 and ** p < .01 vs . model or control group. ns, not significant.

    Article Snippet: The Bacteroides thetaiotaomicron ( B. theta ) strain ATCC 29,148 was derived from ATCC and cultured on blood agar at 37°C under strictly anaerobic conditions.

    Techniques: Activity Assay

    Bacteroides thetaiotaomicron ( B. theta ) administration alleviates nonalcoholic fatty liver disease (NAFLD) in HFD-induced mice through multiple mechanisms. A high-fat diet-induced fatty liver in mice accompanied by dysbiosis of gut microbiota and its metabolites. B. theta administration resulted in several beneficial effects, as indicated by the following changes: 1) increase in microbiota diversity and decrease in Firmicutes/Bacteroidetes (F/B) ratio; 2) enhancement of gut folate biosynthesis and hepatic folate metabolism; 3) increase in hepatic polyunsaturated fatty acids (PUFAs) and decrease in monounsaturated fatty acids (MUFAs) levels, with decreased PUFA/MUFA ratio. These multiple mechanisms collectively contribute to the therapeutic effects of B. theta on the development of NAFLD.

    Journal: Gut Microbes

    Article Title: Bacteroides thetaiotaomicron ameliorates mouse hepatic steatosis through regulating gut microbial composition, gut-liver folate and unsaturated fatty acids metabolism

    doi: 10.1080/19490976.2024.2304159

    Figure Lengend Snippet: Bacteroides thetaiotaomicron ( B. theta ) administration alleviates nonalcoholic fatty liver disease (NAFLD) in HFD-induced mice through multiple mechanisms. A high-fat diet-induced fatty liver in mice accompanied by dysbiosis of gut microbiota and its metabolites. B. theta administration resulted in several beneficial effects, as indicated by the following changes: 1) increase in microbiota diversity and decrease in Firmicutes/Bacteroidetes (F/B) ratio; 2) enhancement of gut folate biosynthesis and hepatic folate metabolism; 3) increase in hepatic polyunsaturated fatty acids (PUFAs) and decrease in monounsaturated fatty acids (MUFAs) levels, with decreased PUFA/MUFA ratio. These multiple mechanisms collectively contribute to the therapeutic effects of B. theta on the development of NAFLD.

    Article Snippet: The Bacteroides thetaiotaomicron ( B. theta ) strain ATCC 29,148 was derived from ATCC and cultured on blood agar at 37°C under strictly anaerobic conditions.

    Techniques: