bacteroides heparinase iii  (New England Biolabs)


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  • 95
    Name:
    Bacteroides Heparinase I
    Description:
    Bacteroides Heparinase I 600 units
    Catalog Number:
    p0735l
    Price:
    312
    Size:
    600 units
    Category:
    Glycosidases
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    Structured Review

    New England Biolabs bacteroides heparinase iii
    Bacteroides Heparinase I
    Bacteroides Heparinase I 600 units
    https://www.bioz.com/result/bacteroides heparinase iii/product/New England Biolabs
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bacteroides heparinase iii - by Bioz Stars, 2020-11
    95/100 stars

    Related Products / Commonly Used Together

    bacteroides heparinases
    cell surface hspgs
    heparinase i

    Images

    1) Product Images from "A Naturally Occurring Polymorphism in the HIV-1 Tat Basic Domain Inhibits Uptake by Bystander Cells and Leads to Reduced Neuroinflammation"

    Article Title: A Naturally Occurring Polymorphism in the HIV-1 Tat Basic Domain Inhibits Uptake by Bystander Cells and Leads to Reduced Neuroinflammation

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-39531-5

    Flow cytometry studies indicate a higher uptake of CPP-R57 over CPP-S57 under a variety of conditions. Various cell types were incubated with fluorescently labeled CPP peptides and the uptake measured by flow cytometry. ( a ) 293T cells exposed to 2 μM of a control peptide, CPP-R57 or CPP-S57 peptides followed by analysis by flow cytometry. Proportional MFI values between experiments (n = 4) were then averaged and plotted on a histogram. Raw MFI values for each sample across different experiments spanned the following ranges: control (1.8–5.5), CPP-R57 (41.9–301.0) and CPP-S57 (11.6–99.0). ( b ) 293T cells and THP-1 cells exposed to increasing concentrations of CPPs as indicated to study dose response. ( c ) 293T cells exposed to 2 μM of CPPs at either 37 °C or 4 °C. Data was combined between different experiments (n = 4), and raw MFI values spanned the following ranges: control (2.8–5.3), CPP-R57 (58.8–266.0) and CPP-S57 (20.0–97.3). ( d ) 293T cells were exposed to CPPs at the indicated concentrations of heparin. ( e ) 293T cells treated for 2 h with the indicated concentrations of Heparinase I enzyme (with Heparinase III enzyme included at 1/17 the concentration of Heparinase I), then exposed to 2 μM of CPP. Data was combined between different experiments (n = 2), and raw MFI values spanned the following ranges: control 0 U (2.1–2.3), CPP-R57 0 U (114.0–145.0), CPP-S57 0 U (18.3–31.9), CPP-R57 1.9 U (64.6–75.1), CPP-S57 1.9 U (7.4–10.9), CPP-R57 3.8 U (46.3–49.9), CPP-S57 3.8 U (6.1–6.8), CPP-R57 7.5 U (34.1–35.4), CPP-S57 7.5 U (5.0–5.2), CPP-R57 15 U (27.8–35.2), and CPP-S57 15 U (4.0–5.0) (***p
    Figure Legend Snippet: Flow cytometry studies indicate a higher uptake of CPP-R57 over CPP-S57 under a variety of conditions. Various cell types were incubated with fluorescently labeled CPP peptides and the uptake measured by flow cytometry. ( a ) 293T cells exposed to 2 μM of a control peptide, CPP-R57 or CPP-S57 peptides followed by analysis by flow cytometry. Proportional MFI values between experiments (n = 4) were then averaged and plotted on a histogram. Raw MFI values for each sample across different experiments spanned the following ranges: control (1.8–5.5), CPP-R57 (41.9–301.0) and CPP-S57 (11.6–99.0). ( b ) 293T cells and THP-1 cells exposed to increasing concentrations of CPPs as indicated to study dose response. ( c ) 293T cells exposed to 2 μM of CPPs at either 37 °C or 4 °C. Data was combined between different experiments (n = 4), and raw MFI values spanned the following ranges: control (2.8–5.3), CPP-R57 (58.8–266.0) and CPP-S57 (20.0–97.3). ( d ) 293T cells were exposed to CPPs at the indicated concentrations of heparin. ( e ) 293T cells treated for 2 h with the indicated concentrations of Heparinase I enzyme (with Heparinase III enzyme included at 1/17 the concentration of Heparinase I), then exposed to 2 μM of CPP. Data was combined between different experiments (n = 2), and raw MFI values spanned the following ranges: control 0 U (2.1–2.3), CPP-R57 0 U (114.0–145.0), CPP-S57 0 U (18.3–31.9), CPP-R57 1.9 U (64.6–75.1), CPP-S57 1.9 U (7.4–10.9), CPP-R57 3.8 U (46.3–49.9), CPP-S57 3.8 U (6.1–6.8), CPP-R57 7.5 U (34.1–35.4), CPP-S57 7.5 U (5.0–5.2), CPP-R57 15 U (27.8–35.2), and CPP-S57 15 U (4.0–5.0) (***p

    Techniques Used: Flow Cytometry, Cytometry, Conditioned Place Preference, Incubation, Labeling, Concentration Assay

    Related Articles

    In Vitro:

    Article Title: Epigallocatechin-3-gallate remodels apolipoprotein A-I amyloid fibrils into soluble oligomers in the presence of heparin
    Article Snippet: .. Binding of heparin to apoA-I fibrils In vitro binding assays of apoA-I fibril-heparin binding were performed using an adaptation of a procedure described previously , in which the amount of GAG remaining unbound at different fibril/GAG concentration ratios was determined using Bacteroides heparinase I (New England Biolabs Ltd., UK). .. The heparinase enzyme cleaves heparin yielding oligosaccharide products containing unsaturated uronic acids, which can be detected using UV spectroscopy at 232 nm.

    Concentration Assay:

    Article Title: Epigallocatechin-3-gallate remodels apolipoprotein A-I amyloid fibrils into soluble oligomers in the presence of heparin
    Article Snippet: .. Binding of heparin to apoA-I fibrils In vitro binding assays of apoA-I fibril-heparin binding were performed using an adaptation of a procedure described previously , in which the amount of GAG remaining unbound at different fibril/GAG concentration ratios was determined using Bacteroides heparinase I (New England Biolabs Ltd., UK). .. The heparinase enzyme cleaves heparin yielding oligosaccharide products containing unsaturated uronic acids, which can be detected using UV spectroscopy at 232 nm.

    Incubation:

    Article Title: Heparin affinity purification of extracellular vesicles
    Article Snippet: .. Beads were then washed three times with PBS 1x and either treated with Bacteroides Heparinase I (New England Biolabs, Ipswich, MA; 60U/ml heparin beads) according to the manufacturer’s recommendations or mock treated (incubation buffer devoid of heparinase) for 1 hr at 30 °C. ..

    other:

    Article Title: Heparin Promotes Cardiac Differentiation of Human Pluripotent Stem Cells in Chemically Defined Albumin‐Free Medium, Enabling Consistent Manufacture of Cardiomyocytes
    Article Snippet: The growth factors, inhibitors, and enzymes used in culture were as follows: activin A (10 ng/ml, catalog no. 338‐AC/CF; R & D Systems, Minneapolis, MN, https://www.rndsystems.com ), BMP4 (10 ng/ml, catalog no. 314‐BP/CF; R & D Systems), fibroblast growth factor 2 (FGF2; 100 ng/ml, catalog no. 100‐18B; Peprotech, Rocky Hill, NJ, https://www.peprotech.com ), transforming growth factor β (TGFβ) 1 (1.74 ng/ml, catalog no. 240‐B/CF; R & D), dorsomorphin (3 μM, catalog no. 04‐0024; Stemgent, Lexington, MA, https://www.stemgent.com ), LDN‐193189 (0.1 μM, catalog no. 04‐0074; Stemgent), PD0325901 (1 μM, catalog no. 04‐0008; Stemgent), SB431542 (3 μM, catalog no. s1067; Selleckchem, Houston, TX, http://www.selleckchem.com ), heparin (catalog no. H3149; Sigma‐Aldrich), heparinase I (catalog no. P0735S; New England Biolabs, Ipswich, MA, https://www.neb.com ).

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Serial in-solution digestion protocol for mass spectrometry-based glycomics and proteomics analysis.
    Article Snippet: .. Advancement in mass spectrometry has revolutionized the field of proteomics. .. Advancement in mass spectrometry has revolutionized the field of proteomics.

    Binding Assay:

    Article Title: Epigallocatechin-3-gallate remodels apolipoprotein A-I amyloid fibrils into soluble oligomers in the presence of heparin
    Article Snippet: .. Binding of heparin to apoA-I fibrils In vitro binding assays of apoA-I fibril-heparin binding were performed using an adaptation of a procedure described previously , in which the amount of GAG remaining unbound at different fibril/GAG concentration ratios was determined using Bacteroides heparinase I (New England Biolabs Ltd., UK). .. The heparinase enzyme cleaves heparin yielding oligosaccharide products containing unsaturated uronic acids, which can be detected using UV spectroscopy at 232 nm.

    Molecular Weight:

    Article Title: Serial in-solution digestion protocol for mass spectrometry-based glycomics and proteomics analysis.
    Article Snippet: .. Advancement in mass spectrometry has revolutionized the field of proteomics. .. Advancement in mass spectrometry has revolutionized the field of proteomics.

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    New England Biolabs bacteroides heparinase iii
    Flow cytometry studies indicate a higher uptake of CPP-R57 over CPP-S57 under a variety of conditions. Various cell types were incubated with fluorescently labeled CPP peptides and the uptake measured by flow cytometry. ( a ) 293T cells exposed to 2 μM of a control peptide, CPP-R57 or CPP-S57 peptides followed by analysis by flow cytometry. Proportional MFI values between experiments (n = 4) were then averaged and plotted on a histogram. Raw MFI values for each sample across different experiments spanned the following ranges: control (1.8–5.5), CPP-R57 (41.9–301.0) and CPP-S57 (11.6–99.0). ( b ) 293T cells and THP-1 cells exposed to increasing concentrations of CPPs as indicated to study dose response. ( c ) 293T cells exposed to 2 μM of CPPs at either 37 °C or 4 °C. Data was combined between different experiments (n = 4), and raw MFI values spanned the following ranges: control (2.8–5.3), CPP-R57 (58.8–266.0) and CPP-S57 (20.0–97.3). ( d ) 293T cells were exposed to CPPs at the indicated concentrations of heparin. ( e ) 293T cells treated for 2 h with the indicated concentrations of <t>Heparinase</t> I enzyme (with Heparinase <t>III</t> enzyme included at 1/17 the concentration of Heparinase I), then exposed to 2 μM of CPP. Data was combined between different experiments (n = 2), and raw MFI values spanned the following ranges: control 0 U (2.1–2.3), CPP-R57 0 U (114.0–145.0), CPP-S57 0 U (18.3–31.9), CPP-R57 1.9 U (64.6–75.1), CPP-S57 1.9 U (7.4–10.9), CPP-R57 3.8 U (46.3–49.9), CPP-S57 3.8 U (6.1–6.8), CPP-R57 7.5 U (34.1–35.4), CPP-S57 7.5 U (5.0–5.2), CPP-R57 15 U (27.8–35.2), and CPP-S57 15 U (4.0–5.0) (***p
    Bacteroides Heparinase Iii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bacteroides heparinase iii/product/New England Biolabs
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bacteroides heparinase iii - by Bioz Stars, 2020-11
    95/100 stars
      Buy from Supplier

    Image Search Results


    Flow cytometry studies indicate a higher uptake of CPP-R57 over CPP-S57 under a variety of conditions. Various cell types were incubated with fluorescently labeled CPP peptides and the uptake measured by flow cytometry. ( a ) 293T cells exposed to 2 μM of a control peptide, CPP-R57 or CPP-S57 peptides followed by analysis by flow cytometry. Proportional MFI values between experiments (n = 4) were then averaged and plotted on a histogram. Raw MFI values for each sample across different experiments spanned the following ranges: control (1.8–5.5), CPP-R57 (41.9–301.0) and CPP-S57 (11.6–99.0). ( b ) 293T cells and THP-1 cells exposed to increasing concentrations of CPPs as indicated to study dose response. ( c ) 293T cells exposed to 2 μM of CPPs at either 37 °C or 4 °C. Data was combined between different experiments (n = 4), and raw MFI values spanned the following ranges: control (2.8–5.3), CPP-R57 (58.8–266.0) and CPP-S57 (20.0–97.3). ( d ) 293T cells were exposed to CPPs at the indicated concentrations of heparin. ( e ) 293T cells treated for 2 h with the indicated concentrations of Heparinase I enzyme (with Heparinase III enzyme included at 1/17 the concentration of Heparinase I), then exposed to 2 μM of CPP. Data was combined between different experiments (n = 2), and raw MFI values spanned the following ranges: control 0 U (2.1–2.3), CPP-R57 0 U (114.0–145.0), CPP-S57 0 U (18.3–31.9), CPP-R57 1.9 U (64.6–75.1), CPP-S57 1.9 U (7.4–10.9), CPP-R57 3.8 U (46.3–49.9), CPP-S57 3.8 U (6.1–6.8), CPP-R57 7.5 U (34.1–35.4), CPP-S57 7.5 U (5.0–5.2), CPP-R57 15 U (27.8–35.2), and CPP-S57 15 U (4.0–5.0) (***p

    Journal: Scientific Reports

    Article Title: A Naturally Occurring Polymorphism in the HIV-1 Tat Basic Domain Inhibits Uptake by Bystander Cells and Leads to Reduced Neuroinflammation

    doi: 10.1038/s41598-019-39531-5

    Figure Lengend Snippet: Flow cytometry studies indicate a higher uptake of CPP-R57 over CPP-S57 under a variety of conditions. Various cell types were incubated with fluorescently labeled CPP peptides and the uptake measured by flow cytometry. ( a ) 293T cells exposed to 2 μM of a control peptide, CPP-R57 or CPP-S57 peptides followed by analysis by flow cytometry. Proportional MFI values between experiments (n = 4) were then averaged and plotted on a histogram. Raw MFI values for each sample across different experiments spanned the following ranges: control (1.8–5.5), CPP-R57 (41.9–301.0) and CPP-S57 (11.6–99.0). ( b ) 293T cells and THP-1 cells exposed to increasing concentrations of CPPs as indicated to study dose response. ( c ) 293T cells exposed to 2 μM of CPPs at either 37 °C or 4 °C. Data was combined between different experiments (n = 4), and raw MFI values spanned the following ranges: control (2.8–5.3), CPP-R57 (58.8–266.0) and CPP-S57 (20.0–97.3). ( d ) 293T cells were exposed to CPPs at the indicated concentrations of heparin. ( e ) 293T cells treated for 2 h with the indicated concentrations of Heparinase I enzyme (with Heparinase III enzyme included at 1/17 the concentration of Heparinase I), then exposed to 2 μM of CPP. Data was combined between different experiments (n = 2), and raw MFI values spanned the following ranges: control 0 U (2.1–2.3), CPP-R57 0 U (114.0–145.0), CPP-S57 0 U (18.3–31.9), CPP-R57 1.9 U (64.6–75.1), CPP-S57 1.9 U (7.4–10.9), CPP-R57 3.8 U (46.3–49.9), CPP-S57 3.8 U (6.1–6.8), CPP-R57 7.5 U (34.1–35.4), CPP-S57 7.5 U (5.0–5.2), CPP-R57 15 U (27.8–35.2), and CPP-S57 15 U (4.0–5.0) (***p

    Article Snippet: The cell surface HSPGs were digested using Heparinase I and Heparinase III enzymes (both cloned from Bacteroides strains; NE Biolabs #P0735S and #P0737S respectively).

    Techniques: Flow Cytometry, Cytometry, Conditioned Place Preference, Incubation, Labeling, Concentration Assay