lambda dna  (New England Biolabs)


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    Name:
    Lambda DNA
    Description:
    Lambda DNA 1 250 ug
    Catalog Number:
    N3011L
    Price:
    276
    Category:
    Genomic DNA
    Size:
    1 250 ug
    Buy from Supplier


    Structured Review

    New England Biolabs lambda dna
    Lambda DNA
    Lambda DNA 1 250 ug
    https://www.bioz.com/result/lambda dna/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lambda dna - by Bioz Stars, 2021-06
    99/100 stars

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    Related Articles

    Purification:

    Article Title: Duplex DNA engagement and RPA oppositely regulate the DNA-unwinding rate of CMG helicase
    Article Snippet: Forked 10-kb linear DNA used in single-molecule assays (Fig. ) was generated by treating λ DNA with ApaI (NEB). .. 10-kb fragment from ApaI-cut λ DNA was purified from a 0.5% agarose gel using Monarch gel extraction kit (NEB). .. We generated the fork end containing a 5′ biotin and a Cy3 on the 3′ dT40 tail by annealing Oligo-Bio-4 and Oligo-Cy3-1, which leaves a 12-nt ssDNA complementary to one end of the 10 kb λ-ApaI fragment.

    Agarose Gel Electrophoresis:

    Article Title: Duplex DNA engagement and RPA oppositely regulate the DNA-unwinding rate of CMG helicase
    Article Snippet: Forked 10-kb linear DNA used in single-molecule assays (Fig. ) was generated by treating λ DNA with ApaI (NEB). .. 10-kb fragment from ApaI-cut λ DNA was purified from a 0.5% agarose gel using Monarch gel extraction kit (NEB). .. We generated the fork end containing a 5′ biotin and a Cy3 on the 3′ dT40 tail by annealing Oligo-Bio-4 and Oligo-Cy3-1, which leaves a 12-nt ssDNA complementary to one end of the 10 kb λ-ApaI fragment.

    Gel Extraction:

    Article Title: Duplex DNA engagement and RPA oppositely regulate the DNA-unwinding rate of CMG helicase
    Article Snippet: Forked 10-kb linear DNA used in single-molecule assays (Fig. ) was generated by treating λ DNA with ApaI (NEB). .. 10-kb fragment from ApaI-cut λ DNA was purified from a 0.5% agarose gel using Monarch gel extraction kit (NEB). .. We generated the fork end containing a 5′ biotin and a Cy3 on the 3′ dT40 tail by annealing Oligo-Bio-4 and Oligo-Cy3-1, which leaves a 12-nt ssDNA complementary to one end of the 10 kb λ-ApaI fragment.

    Marker:

    Article Title: Simple and Cost-Effective Restriction Endonuclease Analysis of Human Adenoviruses
    Article Snippet: After electrophoresis, the DNA in the gel was stained for 30 minutes in GelRed (Biotium, Hayward, CA) solution made in TAE buffer. .. Lambda DNA-HindIII digest marker (New England Biolabs) photographs were taken under UV light. .. For electrophoresis, a prototype strain was always added as a control.

    Article Title: WRN Controls Formation of Extrachromosomal Telomeric Circles and Is Required for TRF2ΔB-Mediated Telomere Shortening ▿-Mediated Telomere Shortening ▿ †
    Article Snippet: After hybridization, excess probe was washed from the membrane and the pattern of hybridization was visualized on X-ray film by autoradiography and PhosphorImager (Molecular Dynamics) scanning of the membrane. .. A circularized lambda DNA marker was generated by ligating HindIII-digested lambda DNA (NEB) at 5 ng/μl overnight at 16°C. .. The ligated lambda DNA was extracted with phenol-chloroform and precipitated with ethanol.

    In Vitro:

    Article Title: High-Throughput Analysis of Global DNA Methylation Using Methyl-Sensitive Digestion
    Article Snippet: .. In vitro methylation of lambda DNA Fully methylated bacteriophage lambda (λ) DNA was acquired by incubating 1 μg of λ DNA in a 20-μl reaction containing 1 U of M.SssI methylase, 1X NEBuffer 2, and freshly-prepared 160 μM S-adenosylmethionine at 37°C for 1 h, followed by heat inactivation of the enzyme at 65°C for 20 min ( ). ..

    Article Title: Spatially Resolved Quantification of Chromatin Condensation through Differential Local Rheology in Cell Nuclei Fluorescence Lifetime Imaging
    Article Snippet: We labeled the constitutive heterochromatin marker Histone H3K9me3 with the primary rabbit polyclonal antibody (ab8898, Abcam, Cambridge, England) and a secondary donkey anti-rabbit antibody (sc-362291, Santa Cruz Biotechnology, Dallas, TX). .. Preparation of DNA in vitro Solutions For the DNA in vitro solution experiments, double-stranded λ-DNA was obtained from New England Biolabs (Ipswich, MA). ..

    Methylation:

    Article Title: High-Throughput Analysis of Global DNA Methylation Using Methyl-Sensitive Digestion
    Article Snippet: .. In vitro methylation of lambda DNA Fully methylated bacteriophage lambda (λ) DNA was acquired by incubating 1 μg of λ DNA in a 20-μl reaction containing 1 U of M.SssI methylase, 1X NEBuffer 2, and freshly-prepared 160 μM S-adenosylmethionine at 37°C for 1 h, followed by heat inactivation of the enzyme at 65°C for 20 min ( ). ..

    Lambda DNA Preparation:

    Article Title: High-Throughput Analysis of Global DNA Methylation Using Methyl-Sensitive Digestion
    Article Snippet: .. In vitro methylation of lambda DNA Fully methylated bacteriophage lambda (λ) DNA was acquired by incubating 1 μg of λ DNA in a 20-μl reaction containing 1 U of M.SssI methylase, 1X NEBuffer 2, and freshly-prepared 160 μM S-adenosylmethionine at 37°C for 1 h, followed by heat inactivation of the enzyme at 65°C for 20 min ( ). ..

    Article Title: WRN Controls Formation of Extrachromosomal Telomeric Circles and Is Required for TRF2ΔB-Mediated Telomere Shortening ▿-Mediated Telomere Shortening ▿ †
    Article Snippet: After hybridization, excess probe was washed from the membrane and the pattern of hybridization was visualized on X-ray film by autoradiography and PhosphorImager (Molecular Dynamics) scanning of the membrane. .. A circularized lambda DNA marker was generated by ligating HindIII-digested lambda DNA (NEB) at 5 ng/μl overnight at 16°C. .. The ligated lambda DNA was extracted with phenol-chloroform and precipitated with ethanol.

    Ligation:

    Article Title: Watching Individual Proteins Acting on Single Molecules of DNA
    Article Snippet: .. Bacteriophage λ DNA (New England Biolabs, Ipswich, MA) is biotinylated by ligation to a 3′-biotinylated 12-mer oligonucleotide (5′-GGGCGGCG ACCT-3′ or 5′-AGGTCGCCGCCC-3′, Operon Technologies, Huntsville, AL) that is complementary to one of the cohesive ends of λ DNA ( ). .. In all the subsequent protocols, the pipetting of solutions containing λ DNA should be performed with cut pipette tips to minimize shearing of the DNA.

    Generated:

    Article Title: WRN Controls Formation of Extrachromosomal Telomeric Circles and Is Required for TRF2ΔB-Mediated Telomere Shortening ▿-Mediated Telomere Shortening ▿ †
    Article Snippet: After hybridization, excess probe was washed from the membrane and the pattern of hybridization was visualized on X-ray film by autoradiography and PhosphorImager (Molecular Dynamics) scanning of the membrane. .. A circularized lambda DNA marker was generated by ligating HindIII-digested lambda DNA (NEB) at 5 ng/μl overnight at 16°C. .. The ligated lambda DNA was extracted with phenol-chloroform and precipitated with ethanol.

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  • 99
    New England Biolabs lambda phage dna
    Application of the real-time DOP-PCR to diverse <t>DNA</t> species. Amplification profiles and their standard curves were obtained from human placental DNA (HPD; A), calf thymus DNA (CTD; B), E. coli DNA (C), and <t>lambda</t> phage DNA (D). Standard DNA samples from 80 fg to 80 ng and a no-template control were amplified. Six independent experiments each comprising triplicate reactions were performed, and typical results of one experiment are presented. Data for 80 ng and NTC were omitted for the plotting of standard curves.
    Lambda Phage Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lambda phage dna/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lambda phage dna - by Bioz Stars, 2021-06
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs bacteriophage λ dna
    RecBCD translocating through, and unwinding, an individual <t>λ</t> DNA molecule. (A) Kymograph showing a YOYO-1 stained λ dsDNA molecule being unwound by a RecBCD molecule bound to the free DNA end. The drawing to the left of the kymograph depicts
    Bacteriophage λ Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bacteriophage λ dna/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bacteriophage λ dna - by Bioz Stars, 2021-06
    99/100 stars
      Buy from Supplier

    Image Search Results


    Application of the real-time DOP-PCR to diverse DNA species. Amplification profiles and their standard curves were obtained from human placental DNA (HPD; A), calf thymus DNA (CTD; B), E. coli DNA (C), and lambda phage DNA (D). Standard DNA samples from 80 fg to 80 ng and a no-template control were amplified. Six independent experiments each comprising triplicate reactions were performed, and typical results of one experiment are presented. Data for 80 ng and NTC were omitted for the plotting of standard curves.

    Journal: PLoS ONE

    Article Title: Quantification of Trace-Level DNA by Real-Time Whole Genome Amplification

    doi: 10.1371/journal.pone.0028661

    Figure Lengend Snippet: Application of the real-time DOP-PCR to diverse DNA species. Amplification profiles and their standard curves were obtained from human placental DNA (HPD; A), calf thymus DNA (CTD; B), E. coli DNA (C), and lambda phage DNA (D). Standard DNA samples from 80 fg to 80 ng and a no-template control were amplified. Six independent experiments each comprising triplicate reactions were performed, and typical results of one experiment are presented. Data for 80 ng and NTC were omitted for the plotting of standard curves.

    Article Snippet: Concentrations of CTD, E. coli DNA, and lambda phage DNA were determined by measuring UV absorbance at 260 nm.

    Techniques: Degenerate Oligonucleotide–primed Polymerase Chain Reaction, Amplification

    Lattice occupancy analysis of lambda DNA. ( a ) MSD-Δ t profile of lambda DNA (Δ t =6.4 ms). The red line shows the theoretical MSD-Δ t profile. ( b ) Frequency histogram of the HE-1Δ t distribution of the experimental (top) and the S r A r simulated replicates (bottom) of lambda DNA. ( c ) Averaged MSD-Δ t profiles of the sub-trajectories captured in the same way as in Fig. 7a (red) and Fig. 7b (blue). The red and blue lines are the theoretical MSD-Δ t profiles. The green line shows the overall, theoretical MSD-Δ t profile of the experimental replicates.

    Journal: Nature Communications

    Article Title: Conserved linear dynamics of single-molecule Brownian motion

    doi: 10.1038/ncomms15675

    Figure Lengend Snippet: Lattice occupancy analysis of lambda DNA. ( a ) MSD-Δ t profile of lambda DNA (Δ t =6.4 ms). The red line shows the theoretical MSD-Δ t profile. ( b ) Frequency histogram of the HE-1Δ t distribution of the experimental (top) and the S r A r simulated replicates (bottom) of lambda DNA. ( c ) Averaged MSD-Δ t profiles of the sub-trajectories captured in the same way as in Fig. 7a (red) and Fig. 7b (blue). The red and blue lines are the theoretical MSD-Δ t profiles. The green line shows the overall, theoretical MSD-Δ t profile of the experimental replicates.

    Article Snippet: Materials Supercoiled ColE1 (6.6 kbp) DNA was obtained from Nippon Gene (Toyama, Japan) whereas the lambda phage DNA was obtained from New England Biolabs (Hitchin, UK).

    Techniques: Lambda DNA Preparation, Mass Spectrometry

    Recombineering at three unique positions within the λ-phage genome. ( a ) Insertion cassettes were designed for three sites targeting dispensable segments of the λ-phage genome (shown in gray). These cassettes are 21.3 kb, 33.5 kb and 45.3 kb away from cosL (designated A, B and C, respectively). ( b ) Schematic of the Red-based recombineering. An arabinose-inducible plasmid supplies the three Red genes. ( c ) Cassettes for efficient recombineering into each of the sites shown in ( a ). The cassettes carry an antibiotic resistance gene that is flanked on both sides by ~200 bp of homology to λ-DNA. ( d ) Recombineering efficiency was scored by colony PCR followed by agarose gels analysis. Successful recombineering generates a smaller PCR fragment. We observed 90–100% insertion efficiency at each of the three target sites.

    Journal: Scientific Reports

    Article Title: Efficient modification of λ-DNA substrates for single-molecule studies

    doi: 10.1038/s41598-017-01984-x

    Figure Lengend Snippet: Recombineering at three unique positions within the λ-phage genome. ( a ) Insertion cassettes were designed for three sites targeting dispensable segments of the λ-phage genome (shown in gray). These cassettes are 21.3 kb, 33.5 kb and 45.3 kb away from cosL (designated A, B and C, respectively). ( b ) Schematic of the Red-based recombineering. An arabinose-inducible plasmid supplies the three Red genes. ( c ) Cassettes for efficient recombineering into each of the sites shown in ( a ). The cassettes carry an antibiotic resistance gene that is flanked on both sides by ~200 bp of homology to λ-DNA. ( d ) Recombineering efficiency was scored by colony PCR followed by agarose gels analysis. Successful recombineering generates a smaller PCR fragment. We observed 90–100% insertion efficiency at each of the three target sites.

    Article Snippet: First, a lysogen was created by packaging λ-phage DNA (cI857ind 1 Sam 7; NEB) into empty phage particles (MaxPlax Lambda Packaging Kit, EpiCentre).

    Techniques: Plasmid Preparation, Polymerase Chain Reaction

    RecBCD translocating through, and unwinding, an individual λ DNA molecule. (A) Kymograph showing a YOYO-1 stained λ dsDNA molecule being unwound by a RecBCD molecule bound to the free DNA end. The drawing to the left of the kymograph depicts

    Journal: Methods in enzymology

    Article Title: Watching Individual Proteins Acting on Single Molecules of DNA

    doi: 10.1016/S0076-6879(10)72007-3

    Figure Lengend Snippet: RecBCD translocating through, and unwinding, an individual λ DNA molecule. (A) Kymograph showing a YOYO-1 stained λ dsDNA molecule being unwound by a RecBCD molecule bound to the free DNA end. The drawing to the left of the kymograph depicts

    Article Snippet: Bacteriophage λ DNA (New England Biolabs, Ipswich, MA) is biotinylated by ligation to a 3′-biotinylated 12-mer oligonucleotide (5′-GGGCGGCG ACCT-3′ or 5′-AGGTCGCCGCCC-3′, Operon Technologies, Huntsville, AL) that is complementary to one of the cohesive ends of λ DNA ( ).

    Techniques: Staining

    Rad54 translocating on a single dsDNA molecule. (A) Schematic illustration of the optically trapped  λ  DNA–bead complex with a bound FITC–Rad54 complex. (B) Kymographs depicting upstream translocation (in the direction opposite

    Journal: Methods in enzymology

    Article Title: Watching Individual Proteins Acting on Single Molecules of DNA

    doi: 10.1016/S0076-6879(10)72007-3

    Figure Lengend Snippet: Rad54 translocating on a single dsDNA molecule. (A) Schematic illustration of the optically trapped λ DNA–bead complex with a bound FITC–Rad54 complex. (B) Kymographs depicting upstream translocation (in the direction opposite

    Article Snippet: Bacteriophage λ DNA (New England Biolabs, Ipswich, MA) is biotinylated by ligation to a 3′-biotinylated 12-mer oligonucleotide (5′-GGGCGGCG ACCT-3′ or 5′-AGGTCGCCGCCC-3′, Operon Technologies, Huntsville, AL) that is complementary to one of the cohesive ends of λ DNA ( ).

    Techniques: Translocation Assay

    Rad51 assembling onto, and dissociating from, a single dsDNA molecule. (A) Kymograph of Rad51 assembly on Cy3-end-labeled  λ  DNA. The schematic on the left side of kymograph depicts the optically trapped bead, initial position of Cy3-end-label

    Journal: Methods in enzymology

    Article Title: Watching Individual Proteins Acting on Single Molecules of DNA

    doi: 10.1016/S0076-6879(10)72007-3

    Figure Lengend Snippet: Rad51 assembling onto, and dissociating from, a single dsDNA molecule. (A) Kymograph of Rad51 assembly on Cy3-end-labeled λ DNA. The schematic on the left side of kymograph depicts the optically trapped bead, initial position of Cy3-end-label

    Article Snippet: Bacteriophage λ DNA (New England Biolabs, Ipswich, MA) is biotinylated by ligation to a 3′-biotinylated 12-mer oligonucleotide (5′-GGGCGGCG ACCT-3′ or 5′-AGGTCGCCGCCC-3′, Operon Technologies, Huntsville, AL) that is complementary to one of the cohesive ends of λ DNA ( ).

    Techniques: Labeling