bacterial culture f nucleatum atcc 25586  (ATCC)


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    Name:
    Fusobacterium nucleatum subsp nucleatum VPI 4355 1612A
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    Catalog Number:
    25586
    Price:
    None
    Applications:
    Media testingQuality control
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    ATCC bacterial culture f nucleatum atcc 25586

    https://www.bioz.com/result/bacterial culture f nucleatum atcc 25586/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bacterial culture f nucleatum atcc 25586 - by Bioz Stars, 2020-09
    99/100 stars

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    Article Title: Fusobacterium Genomics Using MinION and Illumina Sequencing Enables Genome Completion and Correction
    Article Snippet: .. In an attempt to create consistency in genomic builds, we rotated the start site of each chromosome to represent the MreC gene (gene FN1496) as it was originally the first gene of the F. nucleatum subsp. nucleatum ATCC 25586 genome build (NCBI GCA_000007325.1 ). highlights how long reads produced by MinION sequences are able to scaffold and effectively cover a bacterial genome. ..

    Expressing:

    Article Title: Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells
    Article Snippet: .. F. nucleatum ATCC 25586 tended to increase the RANKL expression at 1 day but not at 3 days, and significantly (P < 0.05), despite discrete, upregulated the OPG expression after both 1 day and 3 days (Figures – ). .. Whereas CTSL had no effects on RANKL and OPG expressions (data not shown), CTSH caused a significant (P < 0.05) upregulation of the F. nucleatum -induced RANKL expression at 3 days and a significant (P < 0.05) downregulation of the F. nucleatum -induced OPG expression at 1 day and 3 days (Figures – ).

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    ATCC f nucleatum atcc 25586
    Inhibitory effects of zinc ions on the growth of oral bacteria (mean ± SD). Streptococcus mutans JCM5705 (A), Streptococcus sobrinus (B), Streptococcus salivarius GTC0215 (C), Streptococcus anginosus FW73 (D), Porphyromonas gingivalis W83 (E), ATCC 33277 (F), FDC 381 (G), Prevotella intermedia ATCC 25611 (H), and Fusobacterium <t>nucleatum</t> <t>ATCC</t> 25586 (I)
    F Nucleatum Atcc 25586, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 46 article reviews
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    84
    ATCC growth conditions f nucleatum strains atcc 25586
    AT3 mammary tumor acceleration by F. nucleatum is immune-mediated. a 1 × 10 6 AT3 cells were injected to the mammary fat pad of 6–7-week-old female C57BL/6 mice. When tumor volume reached 500 mm 3 , mice were IV injected with PBS vehicle or with 5 × 10 7 F. nucleatum ATCC 23726 (726). At day 8 post injection, mice were sacrificed, tumors were harvested subjected to flow cytometry and abundance of NK cells, CD4+ T cells and CD8+ T cells was determined. Boxplot whiskers represent extrema, box bounds represent upper and lower quartiles, and center-line represents the median value. n.s.: non-significant. ** p = 0.00216, * p = 0.0152, two-tailed Mann–Whitney (each symbol represents the mean of two technical replicates, n = 6 per group). b 1 × 10 5 AT3 or GFP-expressing AT3 cells were injected to the mammary fat pad of 6–7-week-old female SCID-beige mice. When tumor volume reached 500 mm 3 , mice were IV injected with PBS vehicle or with 5 × 10 7 F. nucleatum ATCC 23726 (726). At day 8 post injection, mice were sacrificed, tumors were harvested and weighed in an analytic weigh. Boxplot whiskers represent extrema, box bounds represent upper and lower quartiles, and center-line represents the median value of two independent experiments. n.s.: non-significant. n = 8 per vehicle group and n = 10 per F. nucleatum -infected group. c Representative picture and densitometry analysis of gelatin zymography of conditioned medium prepared from mouse mammary tumor cell line AT3 or from the human breast cancer cell line MDA-MB-231 that were co-cultured or not, with F. nucleatum strains <t>ATCC</t> 25586 or ATCC 23726. Bars indicate mean ± SEM of six independent experiments. * p = 0.031 two-tailed paired Wilcoxon. Note that mouse MMP-9 is larger than that of human. Source data are provided as a Source data file.
    Growth Conditions F Nucleatum Strains Atcc 25586, supplied by ATCC, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC bacterial culture fusobacterium nucleatum
    The antibacterial effects of mHA, CS, mHA/CS, and rhAm on 10 4 CFU/mL F. <t>nucleatum</t> . (A) Tryptic Soy Broth culture (baseline control), (B) 2.25 mg/mL mHA/CS, (C) 4.5 mg/mL mHA/CS, (D) 1.5 mg/mL CS, (E) 3 mg/mL mHA, and (F) 20μg/mL rhAm (statistically significant against baseline control, ** p
    Bacterial Culture Fusobacterium Nucleatum, supplied by ATCC, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Inhibitory effects of zinc ions on the growth of oral bacteria (mean ± SD). Streptococcus mutans JCM5705 (A), Streptococcus sobrinus (B), Streptococcus salivarius GTC0215 (C), Streptococcus anginosus FW73 (D), Porphyromonas gingivalis W83 (E), ATCC 33277 (F), FDC 381 (G), Prevotella intermedia ATCC 25611 (H), and Fusobacterium nucleatum ATCC 25586 (I)

    Journal: Journal of Applied Oral Science

    Article Title: Two mechanisms of oral malodor inhibition by zinc ions

    doi: 10.1590/1678-7757-2017-0161

    Figure Lengend Snippet: Inhibitory effects of zinc ions on the growth of oral bacteria (mean ± SD). Streptococcus mutans JCM5705 (A), Streptococcus sobrinus (B), Streptococcus salivarius GTC0215 (C), Streptococcus anginosus FW73 (D), Porphyromonas gingivalis W83 (E), ATCC 33277 (F), FDC 381 (G), Prevotella intermedia ATCC 25611 (H), and Fusobacterium nucleatum ATCC 25586 (I)

    Article Snippet: Inhibitory effects of zinc ions on the growth of oral bacteria Among the tested oral bacteria, F. nucleatum ATCC 25586 was the most sensitive, as it was suppressed by 0.001% zinc ions in the medium ( ).

    Techniques:

    AT3 mammary tumor acceleration by F. nucleatum is immune-mediated. a 1 × 10 6 AT3 cells were injected to the mammary fat pad of 6–7-week-old female C57BL/6 mice. When tumor volume reached 500 mm 3 , mice were IV injected with PBS vehicle or with 5 × 10 7 F. nucleatum ATCC 23726 (726). At day 8 post injection, mice were sacrificed, tumors were harvested subjected to flow cytometry and abundance of NK cells, CD4+ T cells and CD8+ T cells was determined. Boxplot whiskers represent extrema, box bounds represent upper and lower quartiles, and center-line represents the median value. n.s.: non-significant. ** p = 0.00216, * p = 0.0152, two-tailed Mann–Whitney (each symbol represents the mean of two technical replicates, n = 6 per group). b 1 × 10 5 AT3 or GFP-expressing AT3 cells were injected to the mammary fat pad of 6–7-week-old female SCID-beige mice. When tumor volume reached 500 mm 3 , mice were IV injected with PBS vehicle or with 5 × 10 7 F. nucleatum ATCC 23726 (726). At day 8 post injection, mice were sacrificed, tumors were harvested and weighed in an analytic weigh. Boxplot whiskers represent extrema, box bounds represent upper and lower quartiles, and center-line represents the median value of two independent experiments. n.s.: non-significant. n = 8 per vehicle group and n = 10 per F. nucleatum -infected group. c Representative picture and densitometry analysis of gelatin zymography of conditioned medium prepared from mouse mammary tumor cell line AT3 or from the human breast cancer cell line MDA-MB-231 that were co-cultured or not, with F. nucleatum strains ATCC 25586 or ATCC 23726. Bars indicate mean ± SEM of six independent experiments. * p = 0.031 two-tailed paired Wilcoxon. Note that mouse MMP-9 is larger than that of human. Source data are provided as a Source data file.

    Journal: Nature Communications

    Article Title: Breast cancer colonization by Fusobacterium nucleatum accelerates tumor growth and metastatic progression

    doi: 10.1038/s41467-020-16967-2

    Figure Lengend Snippet: AT3 mammary tumor acceleration by F. nucleatum is immune-mediated. a 1 × 10 6 AT3 cells were injected to the mammary fat pad of 6–7-week-old female C57BL/6 mice. When tumor volume reached 500 mm 3 , mice were IV injected with PBS vehicle or with 5 × 10 7 F. nucleatum ATCC 23726 (726). At day 8 post injection, mice were sacrificed, tumors were harvested subjected to flow cytometry and abundance of NK cells, CD4+ T cells and CD8+ T cells was determined. Boxplot whiskers represent extrema, box bounds represent upper and lower quartiles, and center-line represents the median value. n.s.: non-significant. ** p = 0.00216, * p = 0.0152, two-tailed Mann–Whitney (each symbol represents the mean of two technical replicates, n = 6 per group). b 1 × 10 5 AT3 or GFP-expressing AT3 cells were injected to the mammary fat pad of 6–7-week-old female SCID-beige mice. When tumor volume reached 500 mm 3 , mice were IV injected with PBS vehicle or with 5 × 10 7 F. nucleatum ATCC 23726 (726). At day 8 post injection, mice were sacrificed, tumors were harvested and weighed in an analytic weigh. Boxplot whiskers represent extrema, box bounds represent upper and lower quartiles, and center-line represents the median value of two independent experiments. n.s.: non-significant. n = 8 per vehicle group and n = 10 per F. nucleatum -infected group. c Representative picture and densitometry analysis of gelatin zymography of conditioned medium prepared from mouse mammary tumor cell line AT3 or from the human breast cancer cell line MDA-MB-231 that were co-cultured or not, with F. nucleatum strains ATCC 25586 or ATCC 23726. Bars indicate mean ± SEM of six independent experiments. * p = 0.031 two-tailed paired Wilcoxon. Note that mouse MMP-9 is larger than that of human. Source data are provided as a Source data file.

    Article Snippet: Bacterial strains and growth conditions F. nucleatum strains ATCC 25586, ATCC 23726, the isogenic Fap2-inactivated mutants K50, D22 and P. gingivalis ATCC 33277 were grown in Wilkins Chalgren broth (Oxoid, UK) or on Columbia agar plates (Oxoid, UK) supplemented with 5% defibrinated sheep blood (Novamed, Israel).

    Techniques: Injection, Mouse Assay, Flow Cytometry, Two Tailed Test, MANN-WHITNEY, Expressing, Infection, Zymography, Multiple Displacement Amplification, Cell Culture

    The antibacterial effects of mHA, CS, mHA/CS, and rhAm on 10 4 CFU/mL F. nucleatum . (A) Tryptic Soy Broth culture (baseline control), (B) 2.25 mg/mL mHA/CS, (C) 4.5 mg/mL mHA/CS, (D) 1.5 mg/mL CS, (E) 3 mg/mL mHA, and (F) 20μg/mL rhAm (statistically significant against baseline control, ** p

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Mesoporous Hydroxyapatite/Chitosan Loaded With Recombinant-Human Amelogenin Could Enhance Antibacterial Effect and Promote Periodontal Regeneration

    doi: 10.3389/fcimb.2020.00180

    Figure Lengend Snippet: The antibacterial effects of mHA, CS, mHA/CS, and rhAm on 10 4 CFU/mL F. nucleatum . (A) Tryptic Soy Broth culture (baseline control), (B) 2.25 mg/mL mHA/CS, (C) 4.5 mg/mL mHA/CS, (D) 1.5 mg/mL CS, (E) 3 mg/mL mHA, and (F) 20μg/mL rhAm (statistically significant against baseline control, ** p

    Article Snippet: Bacterial Culture Fusobacterium nucleatum (F. nucleatum ; ATCC 25586) and Porphyromonas gingivalis (P. gingivalis ; ATCC 33277) were provided by the Shanghai Research Institute of Stomatology and the Shanghai Key Laboratory of Stomatology, Shanghai Ninth People's Hospital (Shanghai, China).

    Techniques:

    Live/dead fluorescent staining images of F. nucleatum biofilms . (A) Tryptic Soy Broth culture (baseline control), (B) 2.25 mg/mL mHA/CS, (C) 4.5 mg/mL mHA/CS, (D) 1.5 mg/mL CS, (E) 3 mg/mL mHA, and (F) 20μg/mL rhAm. Dead cells were stained red, whereas live bacteria were stained green (scale bar 50μm).

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Mesoporous Hydroxyapatite/Chitosan Loaded With Recombinant-Human Amelogenin Could Enhance Antibacterial Effect and Promote Periodontal Regeneration

    doi: 10.3389/fcimb.2020.00180

    Figure Lengend Snippet: Live/dead fluorescent staining images of F. nucleatum biofilms . (A) Tryptic Soy Broth culture (baseline control), (B) 2.25 mg/mL mHA/CS, (C) 4.5 mg/mL mHA/CS, (D) 1.5 mg/mL CS, (E) 3 mg/mL mHA, and (F) 20μg/mL rhAm. Dead cells were stained red, whereas live bacteria were stained green (scale bar 50μm).

    Article Snippet: Bacterial Culture Fusobacterium nucleatum (F. nucleatum ; ATCC 25586) and Porphyromonas gingivalis (P. gingivalis ; ATCC 33277) were provided by the Shanghai Research Institute of Stomatology and the Shanghai Key Laboratory of Stomatology, Shanghai Ninth People's Hospital (Shanghai, China).

    Techniques: Staining