bacterial alkaline phosphatase bap  (TaKaRa)


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    TaKaRa bacterial alkaline phosphatase bap
    Bacterial Alkaline Phosphatase Bap, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore bacterial alkaline phosphatase bap
    Bacterial Alkaline Phosphatase Bap, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore bacterial alkaline phosphatase bap
    Bacterial Alkaline Phosphatase Bap, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Addgene inc bacterial alkaline phosphatase bap ha
    Binding <t>of</t> <t>PRRT2</t> deletion mutants to Nav1.2 and Nav1.1 channels . A , representative immunoblot of co-immunoprecipitation of PRRT2 variants. HA-tagged bacterial alkaline phosphatase <t>(BAP),</t> HA-tagged full-length PRRT2 (PRRT2-FL), or its deletion mutants PRRT2ΔN and PRRT2ΔC were expressed in naive Hek293 cells and purified by HA-immunoprecipitation. The extract of Hek293 stable clones expressing either human Nav1.2 or human Nav1.1 was added to the HA-immunoprecipitated BAP, PRRT2, or PRRT2 deletion mutants. Cell lysates (INPUT, 10 μg protein) and samples immunoprecipitated by anti-HA beads were analyzed by Western blotting with pan-Nav and HA antibodies. Molecular mass standards are reported on the right. The representative blots were cut from the same gel. B , quantification of the immunoreactive signals in PRRT2-HA immunoprecipitates. Box plots of n = 6 and 4 independent experiments for Hek-Nav1.2 and Hek-Nav1.1, respectively. ∗ p < 0.05, ∗∗∗ p < 0.001 versus full-length PRRT2; ° p < 0.05, °°° p < 0.001 Nav1.1 versus Nav1.2 for each PRRT2 variant. Two-way ANOVA/Fisher’s tests. PRRT2 , proline-rich transmembrane protein 2.
    Bacterial Alkaline Phosphatase Bap Ha, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "The intramembrane COOH-terminal domain of PRRT2 regulates voltage-dependent Na + channels"

    Article Title: The intramembrane COOH-terminal domain of PRRT2 regulates voltage-dependent Na + channels

    Journal: The Journal of Biological Chemistry

    doi: 10.1016/j.jbc.2023.104632

    Binding of PRRT2 deletion mutants to Nav1.2 and Nav1.1 channels . A , representative immunoblot of co-immunoprecipitation of PRRT2 variants. HA-tagged bacterial alkaline phosphatase (BAP), HA-tagged full-length PRRT2 (PRRT2-FL), or its deletion mutants PRRT2ΔN and PRRT2ΔC were expressed in naive Hek293 cells and purified by HA-immunoprecipitation. The extract of Hek293 stable clones expressing either human Nav1.2 or human Nav1.1 was added to the HA-immunoprecipitated BAP, PRRT2, or PRRT2 deletion mutants. Cell lysates (INPUT, 10 μg protein) and samples immunoprecipitated by anti-HA beads were analyzed by Western blotting with pan-Nav and HA antibodies. Molecular mass standards are reported on the right. The representative blots were cut from the same gel. B , quantification of the immunoreactive signals in PRRT2-HA immunoprecipitates. Box plots of n = 6 and 4 independent experiments for Hek-Nav1.2 and Hek-Nav1.1, respectively. ∗ p < 0.05, ∗∗∗ p < 0.001 versus full-length PRRT2; ° p < 0.05, °°° p < 0.001 Nav1.1 versus Nav1.2 for each PRRT2 variant. Two-way ANOVA/Fisher’s tests. PRRT2 , proline-rich transmembrane protein 2.
    Figure Legend Snippet: Binding of PRRT2 deletion mutants to Nav1.2 and Nav1.1 channels . A , representative immunoblot of co-immunoprecipitation of PRRT2 variants. HA-tagged bacterial alkaline phosphatase (BAP), HA-tagged full-length PRRT2 (PRRT2-FL), or its deletion mutants PRRT2ΔN and PRRT2ΔC were expressed in naive Hek293 cells and purified by HA-immunoprecipitation. The extract of Hek293 stable clones expressing either human Nav1.2 or human Nav1.1 was added to the HA-immunoprecipitated BAP, PRRT2, or PRRT2 deletion mutants. Cell lysates (INPUT, 10 μg protein) and samples immunoprecipitated by anti-HA beads were analyzed by Western blotting with pan-Nav and HA antibodies. Molecular mass standards are reported on the right. The representative blots were cut from the same gel. B , quantification of the immunoreactive signals in PRRT2-HA immunoprecipitates. Box plots of n = 6 and 4 independent experiments for Hek-Nav1.2 and Hek-Nav1.1, respectively. ∗ p < 0.05, ∗∗∗ p < 0.001 versus full-length PRRT2; ° p < 0.05, °°° p < 0.001 Nav1.1 versus Nav1.2 for each PRRT2 variant. Two-way ANOVA/Fisher’s tests. PRRT2 , proline-rich transmembrane protein 2.

    Techniques Used: Binding Assay, Western Blot, Immunoprecipitation, Purification, Clone Assay, Expressing, Variant Assay

    bacterial alkaline phosphatase bap  (Thermo Fisher)


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    Thermo Fisher bacterial alkaline phosphatase bap
    Bacterial Alkaline Phosphatase Bap, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bacterial alkaline phosphatase bap  (Thermo Fisher)


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    Thermo Fisher bacterial alkaline phosphatase bap
    Bacterial Alkaline Phosphatase Bap, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bacterial alkaline phosphatase bap  (Thermo Fisher)


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    Thermo Fisher bacterial alkaline phosphatase bap
    Bacterial Alkaline Phosphatase Bap, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bacterial alkaline phosphatase bap  (Thermo Fisher)


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    Thermo Fisher bacterial alkaline phosphatase bap
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    bacterial alkaline phosphatase bap  (Thermo Fisher)


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    Thermo Fisher bacterial alkaline phosphatase bap
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    bacterial alkaline phosphatase bap  (Thermo Fisher)


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    Thermo Fisher bacterial alkaline phosphatase bap
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    bacterial alkaline phosphatase bap  (TaKaRa)


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    TaKaRa bacterial alkaline phosphatase bap
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    TaKaRa bacterial alkaline phosphatase bap
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    Millipore bacterial alkaline phosphatase bap
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    Addgene inc bacterial alkaline phosphatase bap ha
    Binding <t>of</t> <t>PRRT2</t> deletion mutants to Nav1.2 and Nav1.1 channels . A , representative immunoblot of co-immunoprecipitation of PRRT2 variants. HA-tagged bacterial alkaline phosphatase <t>(BAP),</t> HA-tagged full-length PRRT2 (PRRT2-FL), or its deletion mutants PRRT2ΔN and PRRT2ΔC were expressed in naive Hek293 cells and purified by HA-immunoprecipitation. The extract of Hek293 stable clones expressing either human Nav1.2 or human Nav1.1 was added to the HA-immunoprecipitated BAP, PRRT2, or PRRT2 deletion mutants. Cell lysates (INPUT, 10 μg protein) and samples immunoprecipitated by anti-HA beads were analyzed by Western blotting with pan-Nav and HA antibodies. Molecular mass standards are reported on the right. The representative blots were cut from the same gel. B , quantification of the immunoreactive signals in PRRT2-HA immunoprecipitates. Box plots of n = 6 and 4 independent experiments for Hek-Nav1.2 and Hek-Nav1.1, respectively. ∗ p < 0.05, ∗∗∗ p < 0.001 versus full-length PRRT2; ° p < 0.05, °°° p < 0.001 Nav1.1 versus Nav1.2 for each PRRT2 variant. Two-way ANOVA/Fisher’s tests. PRRT2 , proline-rich transmembrane protein 2.
    Bacterial Alkaline Phosphatase Bap Ha, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher bacterial alkaline phosphatase bap
    Binding <t>of</t> <t>PRRT2</t> deletion mutants to Nav1.2 and Nav1.1 channels . A , representative immunoblot of co-immunoprecipitation of PRRT2 variants. HA-tagged bacterial alkaline phosphatase <t>(BAP),</t> HA-tagged full-length PRRT2 (PRRT2-FL), or its deletion mutants PRRT2ΔN and PRRT2ΔC were expressed in naive Hek293 cells and purified by HA-immunoprecipitation. The extract of Hek293 stable clones expressing either human Nav1.2 or human Nav1.1 was added to the HA-immunoprecipitated BAP, PRRT2, or PRRT2 deletion mutants. Cell lysates (INPUT, 10 μg protein) and samples immunoprecipitated by anti-HA beads were analyzed by Western blotting with pan-Nav and HA antibodies. Molecular mass standards are reported on the right. The representative blots were cut from the same gel. B , quantification of the immunoreactive signals in PRRT2-HA immunoprecipitates. Box plots of n = 6 and 4 independent experiments for Hek-Nav1.2 and Hek-Nav1.1, respectively. ∗ p < 0.05, ∗∗∗ p < 0.001 versus full-length PRRT2; ° p < 0.05, °°° p < 0.001 Nav1.1 versus Nav1.2 for each PRRT2 variant. Two-way ANOVA/Fisher’s tests. PRRT2 , proline-rich transmembrane protein 2.
    Bacterial Alkaline Phosphatase Bap, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Binding of PRRT2 deletion mutants to Nav1.2 and Nav1.1 channels . A , representative immunoblot of co-immunoprecipitation of PRRT2 variants. HA-tagged bacterial alkaline phosphatase (BAP), HA-tagged full-length PRRT2 (PRRT2-FL), or its deletion mutants PRRT2ΔN and PRRT2ΔC were expressed in naive Hek293 cells and purified by HA-immunoprecipitation. The extract of Hek293 stable clones expressing either human Nav1.2 or human Nav1.1 was added to the HA-immunoprecipitated BAP, PRRT2, or PRRT2 deletion mutants. Cell lysates (INPUT, 10 μg protein) and samples immunoprecipitated by anti-HA beads were analyzed by Western blotting with pan-Nav and HA antibodies. Molecular mass standards are reported on the right. The representative blots were cut from the same gel. B , quantification of the immunoreactive signals in PRRT2-HA immunoprecipitates. Box plots of n = 6 and 4 independent experiments for Hek-Nav1.2 and Hek-Nav1.1, respectively. ∗ p < 0.05, ∗∗∗ p < 0.001 versus full-length PRRT2; ° p < 0.05, °°° p < 0.001 Nav1.1 versus Nav1.2 for each PRRT2 variant. Two-way ANOVA/Fisher’s tests. PRRT2 , proline-rich transmembrane protein 2.

    Journal: The Journal of Biological Chemistry

    Article Title: The intramembrane COOH-terminal domain of PRRT2 regulates voltage-dependent Na + channels

    doi: 10.1016/j.jbc.2023.104632

    Figure Lengend Snippet: Binding of PRRT2 deletion mutants to Nav1.2 and Nav1.1 channels . A , representative immunoblot of co-immunoprecipitation of PRRT2 variants. HA-tagged bacterial alkaline phosphatase (BAP), HA-tagged full-length PRRT2 (PRRT2-FL), or its deletion mutants PRRT2ΔN and PRRT2ΔC were expressed in naive Hek293 cells and purified by HA-immunoprecipitation. The extract of Hek293 stable clones expressing either human Nav1.2 or human Nav1.1 was added to the HA-immunoprecipitated BAP, PRRT2, or PRRT2 deletion mutants. Cell lysates (INPUT, 10 μg protein) and samples immunoprecipitated by anti-HA beads were analyzed by Western blotting with pan-Nav and HA antibodies. Molecular mass standards are reported on the right. The representative blots were cut from the same gel. B , quantification of the immunoreactive signals in PRRT2-HA immunoprecipitates. Box plots of n = 6 and 4 independent experiments for Hek-Nav1.2 and Hek-Nav1.1, respectively. ∗ p < 0.05, ∗∗∗ p < 0.001 versus full-length PRRT2; ° p < 0.05, °°° p < 0.001 Nav1.1 versus Nav1.2 for each PRRT2 variant. Two-way ANOVA/Fisher’s tests. PRRT2 , proline-rich transmembrane protein 2.

    Article Snippet: The human PRRT2-based constructs used in the study are the following: (i) PRRT2-HA, i.e. , pKH3-PRRT2-HA vector encoding for full-length PRRT2 with 3XHA tag fused at the COOH terminus ( ); (ii) bacterial alkaline phosphatase (BAP)-HA, i.e., pKH3-BAP-HA vector encoding for BAP with 3XHA tag fused at the COOH terminus ( ); (iii) PRRT2ΔN-HA, i.e., pKH3-PRRT2ΔN-HA encoding for the PRRT2 membrane domain (amino acids 261–340) with 3XHA tag fused at COOH terminus ( ); (iv) PRRT2ΔC-HA, i.e ., pKH3-PRRT2ΔN-HA encoding for the PRRT2 cytosolic domain (amino acids 1–260) fused, for membrane targeting, to the second membrane domain of IFTM1(a kind gift of dr. Jacob S. Yount; RRID: Addgene_58415; ( )).

    Techniques: Binding Assay, Western Blot, Immunoprecipitation, Purification, Clone Assay, Expressing, Variant Assay