Journal: bioRxiv

Article Title: Intra-ocular Predation of Fluoroquinolone-Resistant Pseudomonas aeruginosa and Serratia marcescens by Predatory Bacteria

doi: 10.1101/2023.09.17.558130

Figure Lengend Snippet: Each point represents a rabbit in all figures. HD100 indicates B. bacteriovorus ; Mica signifies M. aeruginovorus . A . Clinical inflammation scores for rabbits 24 hours post-inoculation. Only the P. aeruginosa group (PAC) and P. aeruginosa with M. aeruginovorus groups were statistical different from the vehicle control, n=5-7. Medians and interquartile ranges (IQR) are shown. Asterisks indicate statistical differences from the PBS group. B . Representative eyes from subject with median clinical scores are shown for each group. C . Intraocular CFU/ml at 24h post-injection (Median and IQR, n=5-7). Asterisks indicate statistical differences from the PaC group, *, p<0.05. The dotted line indicates the inoculum of PaC introduced into the eye. D . IL-1β cytokine measured by ELISA, means and standard deviations (SD) are shown, (n=5-7). E . TNFα cytokine measured by ELISA, means and standard deviations (SD) are shown, (n=5-7). Asterisks indicate differences from the PBS group. *, p<0.05; **, p<0.01; ***, p<0.001; ****, p<0.0001.

Article Snippet: The predatory bacteria Bdellovibrio bacteriovorus HD100 (ATCC 15356) [ , ] and Micavibrio aeruginosavorus ARL-13 [ ] were used in the study.

Techniques: Injection, Enzyme-linked Immunosorbent Assay