bacillus pumilus spores  (ATCC)


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    ATCC bacillus pumilus spores
    Bacillus Pumilus Spores, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bacillus pumilus spores  (ATCC)


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    ATCC bacillus pumilus spores
    Bacillus Pumilus Spores, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bacillus pumilus spores  (ATCC)


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    ATCC bacillus pumilus spores
    Bacillus Pumilus Spores, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    2017 e coli k12 atcc 29425 ms2 coliphage human adenovirus type 2 atcc vr 846 bacillus pumilus atcc 27142 spores 260 280 260  (ATCC)


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    ATCC 2017 e coli k12 atcc 29425 ms2 coliphage human adenovirus type 2 atcc vr 846 bacillus pumilus atcc 27142 spores 260 280 260
    2017 E Coli K12 Atcc 29425 Ms2 Coliphage Human Adenovirus Type 2 Atcc Vr 846 Bacillus Pumilus Atcc 27142 Spores 260 280 260, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    15246 saccharomyces cerevisiae atcc 4098 bacillus pumilus spores  (ATCC)


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    ATCC 15246 saccharomyces cerevisiae atcc 4098 bacillus pumilus spores
    15246 Saccharomyces Cerevisiae Atcc 4098 Bacillus Pumilus Spores, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bacillus pumilus spores atcc 27142  (ATCC)


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    ATCC bacillus pumilus spores atcc 27142
    Bacillus Pumilus Spores Atcc 27142, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bacillus pumilis spores cn 607  (ATCC)


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    ATCC bacillus pumilis spores cn 607
    Bacillus Pumilis Spores Cn 607, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    7061 spore protein extracts  (ATCC)


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    ATCC 7061 spore protein extracts
    SDS-PAGE analysis of proteins extracted from intact and decoated <t>B.</t> <t>pumilus</t> spores. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC <t>7061;</t> 1A and 2A, intact spore protein extracts; 1B and 2B, decoated spore protein extracts; 1C and 2C, coat protein extracts. Lanes 1A and 1B were cut into ∼1.5-mm slices from the bottom to the top for protein identification by LC-MS/MS. A similar amount of protein (∼10 μg) was loaded into each well. The black arrowheads indicate a sporulation-related manganese catalase (YjqC). The white arrowheads indicate the second manganese catalase (BPUM_1305). The numbers indicate the excised PAGE bands.
    7061 Spore Protein Extracts, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Protection of Bacillus pumilus Spores by Catalases"

    Article Title: Protection of Bacillus pumilus Spores by Catalases

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.01211-12

    SDS-PAGE analysis of proteins extracted from intact and decoated B. pumilus spores. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 1A and 2A, intact spore protein extracts; 1B and 2B, decoated spore protein extracts; 1C and 2C, coat protein extracts. Lanes 1A and 1B were cut into ∼1.5-mm slices from the bottom to the top for protein identification by LC-MS/MS. A similar amount of protein (∼10 μg) was loaded into each well. The black arrowheads indicate a sporulation-related manganese catalase (YjqC). The white arrowheads indicate the second manganese catalase (BPUM_1305). The numbers indicate the excised PAGE bands.
    Figure Legend Snippet: SDS-PAGE analysis of proteins extracted from intact and decoated B. pumilus spores. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 1A and 2A, intact spore protein extracts; 1B and 2B, decoated spore protein extracts; 1C and 2C, coat protein extracts. Lanes 1A and 1B were cut into ∼1.5-mm slices from the bottom to the top for protein identification by LC-MS/MS. A similar amount of protein (∼10 μg) was loaded into each well. The black arrowheads indicate a sporulation-related manganese catalase (YjqC). The white arrowheads indicate the second manganese catalase (BPUM_1305). The numbers indicate the excised PAGE bands.

    Techniques Used: SDS Page, Liquid Chromatography with Mass Spectroscopy

    SDS-PAGE analysis of B. pumilus spore protein extracts. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 3, BG-B79; 4, 8A4; 5, 8A6; 6, 14A1. A similar amount of protein was loaded into each 12% SDS-PAGE well. The rectangles (∼1 cm) were excised, digested in the gel with trypsin, and sequenced. The arrowheads indicate the sporulation-related manganese catalase (YjqC). BPUM_1305 was identified in all of the gel extracts tested.
    Figure Legend Snippet: SDS-PAGE analysis of B. pumilus spore protein extracts. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 3, BG-B79; 4, 8A4; 5, 8A6; 6, 14A1. A similar amount of protein was loaded into each 12% SDS-PAGE well. The rectangles (∼1 cm) were excised, digested in the gel with trypsin, and sequenced. The arrowheads indicate the sporulation-related manganese catalase (YjqC). BPUM_1305 was identified in all of the gel extracts tested.

    Techniques Used: SDS Page

    Manganese catalases identified by SDS-PAGE and LC-MS/MS in B.  pumilus  spores
    Figure Legend Snippet: Manganese catalases identified by SDS-PAGE and LC-MS/MS in B. pumilus spores

    Techniques Used:

    Catalase specific activities detected in B.  pumilus  spore suspensions
    Figure Legend Snippet: Catalase specific activities detected in B. pumilus spore suspensions

    Techniques Used: Activity Assay

    7061 spores  (ATCC)


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    ATCC 7061 spores
    SDS-PAGE analysis of proteins extracted from intact and decoated B. pumilus spores. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC <t>7061;</t> 1A and 2A, intact spore protein extracts; 1B and 2B, decoated spore protein extracts; 1C and 2C, coat protein extracts. Lanes 1A and 1B were cut into ∼1.5-mm slices from the bottom to the top for protein identification by LC-MS/MS. A similar amount of protein (∼10 μg) was loaded into each well. The black arrowheads indicate a sporulation-related <t>manganese</t> <t>catalase</t> (YjqC). The white arrowheads indicate the second manganese catalase (BPUM_1305). The numbers indicate the excised PAGE bands.
    7061 Spores, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Protection of Bacillus pumilus Spores by Catalases"

    Article Title: Protection of Bacillus pumilus Spores by Catalases

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.01211-12

    SDS-PAGE analysis of proteins extracted from intact and decoated B. pumilus spores. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 1A and 2A, intact spore protein extracts; 1B and 2B, decoated spore protein extracts; 1C and 2C, coat protein extracts. Lanes 1A and 1B were cut into ∼1.5-mm slices from the bottom to the top for protein identification by LC-MS/MS. A similar amount of protein (∼10 μg) was loaded into each well. The black arrowheads indicate a sporulation-related manganese catalase (YjqC). The white arrowheads indicate the second manganese catalase (BPUM_1305). The numbers indicate the excised PAGE bands.
    Figure Legend Snippet: SDS-PAGE analysis of proteins extracted from intact and decoated B. pumilus spores. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 1A and 2A, intact spore protein extracts; 1B and 2B, decoated spore protein extracts; 1C and 2C, coat protein extracts. Lanes 1A and 1B were cut into ∼1.5-mm slices from the bottom to the top for protein identification by LC-MS/MS. A similar amount of protein (∼10 μg) was loaded into each well. The black arrowheads indicate a sporulation-related manganese catalase (YjqC). The white arrowheads indicate the second manganese catalase (BPUM_1305). The numbers indicate the excised PAGE bands.

    Techniques Used: SDS Page, Liquid Chromatography with Mass Spectroscopy

    SDS-PAGE analysis of B. pumilus spore protein extracts. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 3, BG-B79; 4, 8A4; 5, 8A6; 6, 14A1. A similar amount of protein was loaded into each 12% SDS-PAGE well. The rectangles (∼1 cm) were excised, digested in the gel with trypsin, and sequenced. The arrowheads indicate the sporulation-related manganese catalase (YjqC). BPUM_1305 was identified in all of the gel extracts tested.
    Figure Legend Snippet: SDS-PAGE analysis of B. pumilus spore protein extracts. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 3, BG-B79; 4, 8A4; 5, 8A6; 6, 14A1. A similar amount of protein was loaded into each 12% SDS-PAGE well. The rectangles (∼1 cm) were excised, digested in the gel with trypsin, and sequenced. The arrowheads indicate the sporulation-related manganese catalase (YjqC). BPUM_1305 was identified in all of the gel extracts tested.

    Techniques Used: SDS Page

     Catalase  specific activities detected in B. pumilus spore suspensions
    Figure Legend Snippet: Catalase specific activities detected in B. pumilus spore suspensions

    Techniques Used: Activity Assay

    7061 spore protein extracts  (ATCC)


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    ATCC 7061 spore protein extracts
    SDS-PAGE analysis of proteins extracted from intact and decoated B. pumilus spores. Lanes: ST, molecular mass standards (sizes in kDa are on the left); <t>1,</t> <t>SAFR-032;</t> 2, ATCC <t>7061;</t> 1A and 2A, intact spore protein extracts; 1B and 2B, decoated spore protein extracts; 1C and 2C, coat protein extracts. Lanes 1A and 1B were cut into ∼1.5-mm slices from the bottom to the top for protein identification by LC-MS/MS. A similar amount of protein (∼10 μg) was loaded into each well. The black arrowheads indicate a sporulation-related manganese catalase (YjqC). The white arrowheads indicate the second manganese catalase (BPUM_1305). The numbers indicate the excised PAGE bands.
    7061 Spore Protein Extracts, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Protection of Bacillus pumilus Spores by Catalases"

    Article Title: Protection of Bacillus pumilus Spores by Catalases

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.01211-12

    SDS-PAGE analysis of proteins extracted from intact and decoated B. pumilus spores. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 1A and 2A, intact spore protein extracts; 1B and 2B, decoated spore protein extracts; 1C and 2C, coat protein extracts. Lanes 1A and 1B were cut into ∼1.5-mm slices from the bottom to the top for protein identification by LC-MS/MS. A similar amount of protein (∼10 μg) was loaded into each well. The black arrowheads indicate a sporulation-related manganese catalase (YjqC). The white arrowheads indicate the second manganese catalase (BPUM_1305). The numbers indicate the excised PAGE bands.
    Figure Legend Snippet: SDS-PAGE analysis of proteins extracted from intact and decoated B. pumilus spores. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 1A and 2A, intact spore protein extracts; 1B and 2B, decoated spore protein extracts; 1C and 2C, coat protein extracts. Lanes 1A and 1B were cut into ∼1.5-mm slices from the bottom to the top for protein identification by LC-MS/MS. A similar amount of protein (∼10 μg) was loaded into each well. The black arrowheads indicate a sporulation-related manganese catalase (YjqC). The white arrowheads indicate the second manganese catalase (BPUM_1305). The numbers indicate the excised PAGE bands.

    Techniques Used: SDS Page, Liquid Chromatography with Mass Spectroscopy

    SDS-PAGE analysis of B. pumilus spore protein extracts. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 3, BG-B79; 4, 8A4; 5, 8A6; 6, 14A1. A similar amount of protein was loaded into each 12% SDS-PAGE well. The rectangles (∼1 cm) were excised, digested in the gel with trypsin, and sequenced. The arrowheads indicate the sporulation-related manganese catalase (YjqC). BPUM_1305 was identified in all of the gel extracts tested.
    Figure Legend Snippet: SDS-PAGE analysis of B. pumilus spore protein extracts. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 3, BG-B79; 4, 8A4; 5, 8A6; 6, 14A1. A similar amount of protein was loaded into each 12% SDS-PAGE well. The rectangles (∼1 cm) were excised, digested in the gel with trypsin, and sequenced. The arrowheads indicate the sporulation-related manganese catalase (YjqC). BPUM_1305 was identified in all of the gel extracts tested.

    Techniques Used: SDS Page

    7061 spore suspensions  (ATCC)


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    ATCC 7061 spore suspensions
    SDS-PAGE analysis of proteins extracted from intact and decoated B. pumilus spores. Lanes: ST, molecular mass standards (sizes in kDa are on the left); <t>1,</t> <t>SAFR-032;</t> 2, ATCC <t>7061;</t> 1A and 2A, intact spore protein extracts; 1B and 2B, decoated spore protein extracts; 1C and 2C, coat protein extracts. Lanes 1A and 1B were cut into ∼1.5-mm slices from the bottom to the top for protein identification by LC-MS/MS. A similar amount of protein (∼10 μg) was loaded into each well. The black arrowheads indicate a sporulation-related manganese catalase (YjqC). The white arrowheads indicate the second manganese catalase (BPUM_1305). The numbers indicate the excised PAGE bands.
    7061 Spore Suspensions, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Protection of Bacillus pumilus Spores by Catalases"

    Article Title: Protection of Bacillus pumilus Spores by Catalases

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.01211-12

    SDS-PAGE analysis of proteins extracted from intact and decoated B. pumilus spores. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 1A and 2A, intact spore protein extracts; 1B and 2B, decoated spore protein extracts; 1C and 2C, coat protein extracts. Lanes 1A and 1B were cut into ∼1.5-mm slices from the bottom to the top for protein identification by LC-MS/MS. A similar amount of protein (∼10 μg) was loaded into each well. The black arrowheads indicate a sporulation-related manganese catalase (YjqC). The white arrowheads indicate the second manganese catalase (BPUM_1305). The numbers indicate the excised PAGE bands.
    Figure Legend Snippet: SDS-PAGE analysis of proteins extracted from intact and decoated B. pumilus spores. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 1A and 2A, intact spore protein extracts; 1B and 2B, decoated spore protein extracts; 1C and 2C, coat protein extracts. Lanes 1A and 1B were cut into ∼1.5-mm slices from the bottom to the top for protein identification by LC-MS/MS. A similar amount of protein (∼10 μg) was loaded into each well. The black arrowheads indicate a sporulation-related manganese catalase (YjqC). The white arrowheads indicate the second manganese catalase (BPUM_1305). The numbers indicate the excised PAGE bands.

    Techniques Used: SDS Page, Liquid Chromatography with Mass Spectroscopy

    SDS-PAGE analysis of B. pumilus spore protein extracts. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 3, BG-B79; 4, 8A4; 5, 8A6; 6, 14A1. A similar amount of protein was loaded into each 12% SDS-PAGE well. The rectangles (∼1 cm) were excised, digested in the gel with trypsin, and sequenced. The arrowheads indicate the sporulation-related manganese catalase (YjqC). BPUM_1305 was identified in all of the gel extracts tested.
    Figure Legend Snippet: SDS-PAGE analysis of B. pumilus spore protein extracts. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 3, BG-B79; 4, 8A4; 5, 8A6; 6, 14A1. A similar amount of protein was loaded into each 12% SDS-PAGE well. The rectangles (∼1 cm) were excised, digested in the gel with trypsin, and sequenced. The arrowheads indicate the sporulation-related manganese catalase (YjqC). BPUM_1305 was identified in all of the gel extracts tested.

    Techniques Used: SDS Page

    Catalase specific activities detected in B. pumilus  spore suspensions
    Figure Legend Snippet: Catalase specific activities detected in B. pumilus spore suspensions

    Techniques Used: Activity Assay

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    95
    ATCC 7061 spore protein extracts
    SDS-PAGE analysis of proteins extracted from intact and decoated <t>B.</t> <t>pumilus</t> spores. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC <t>7061;</t> 1A and 2A, intact spore protein extracts; 1B and 2B, decoated spore protein extracts; 1C and 2C, coat protein extracts. Lanes 1A and 1B were cut into ∼1.5-mm slices from the bottom to the top for protein identification by LC-MS/MS. A similar amount of protein (∼10 μg) was loaded into each well. The black arrowheads indicate a sporulation-related manganese catalase (YjqC). The white arrowheads indicate the second manganese catalase (BPUM_1305). The numbers indicate the excised PAGE bands.
    7061 Spore Protein Extracts, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7061 spore protein extracts/product/ATCC
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    7061 spore protein extracts - by Bioz Stars, 2024-04
    95/100 stars
      Buy from Supplier

    95
    ATCC 7061 spores
    SDS-PAGE analysis of proteins extracted from intact and decoated B. pumilus spores. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC <t>7061;</t> 1A and 2A, intact spore protein extracts; 1B and 2B, decoated spore protein extracts; 1C and 2C, coat protein extracts. Lanes 1A and 1B were cut into ∼1.5-mm slices from the bottom to the top for protein identification by LC-MS/MS. A similar amount of protein (∼10 μg) was loaded into each well. The black arrowheads indicate a sporulation-related <t>manganese</t> <t>catalase</t> (YjqC). The white arrowheads indicate the second manganese catalase (BPUM_1305). The numbers indicate the excised PAGE bands.
    7061 Spores, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7061 spores/product/ATCC
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    7061 spores - by Bioz Stars, 2024-04
    95/100 stars
      Buy from Supplier

    95
    ATCC 7061 spore suspensions
    SDS-PAGE analysis of proteins extracted from intact and decoated B. pumilus spores. Lanes: ST, molecular mass standards (sizes in kDa are on the left); <t>1,</t> <t>SAFR-032;</t> 2, ATCC <t>7061;</t> 1A and 2A, intact spore protein extracts; 1B and 2B, decoated spore protein extracts; 1C and 2C, coat protein extracts. Lanes 1A and 1B were cut into ∼1.5-mm slices from the bottom to the top for protein identification by LC-MS/MS. A similar amount of protein (∼10 μg) was loaded into each well. The black arrowheads indicate a sporulation-related manganese catalase (YjqC). The white arrowheads indicate the second manganese catalase (BPUM_1305). The numbers indicate the excised PAGE bands.
    7061 Spore Suspensions, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7061 spore suspensions/product/ATCC
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    7061 spore suspensions - by Bioz Stars, 2024-04
    95/100 stars
      Buy from Supplier

    Image Search Results


    SDS-PAGE analysis of proteins extracted from intact and decoated B. pumilus spores. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 1A and 2A, intact spore protein extracts; 1B and 2B, decoated spore protein extracts; 1C and 2C, coat protein extracts. Lanes 1A and 1B were cut into ∼1.5-mm slices from the bottom to the top for protein identification by LC-MS/MS. A similar amount of protein (∼10 μg) was loaded into each well. The black arrowheads indicate a sporulation-related manganese catalase (YjqC). The white arrowheads indicate the second manganese catalase (BPUM_1305). The numbers indicate the excised PAGE bands.

    Journal: Applied and Environmental Microbiology

    Article Title: Protection of Bacillus pumilus Spores by Catalases

    doi: 10.1128/AEM.01211-12

    Figure Lengend Snippet: SDS-PAGE analysis of proteins extracted from intact and decoated B. pumilus spores. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 1A and 2A, intact spore protein extracts; 1B and 2B, decoated spore protein extracts; 1C and 2C, coat protein extracts. Lanes 1A and 1B were cut into ∼1.5-mm slices from the bottom to the top for protein identification by LC-MS/MS. A similar amount of protein (∼10 μg) was loaded into each well. The black arrowheads indicate a sporulation-related manganese catalase (YjqC). The white arrowheads indicate the second manganese catalase (BPUM_1305). The numbers indicate the excised PAGE bands.

    Article Snippet: B. pumilus SAFR-032 and ATCC 7061 spore protein extracts were analyzed by SDS-PAGE.

    Techniques: SDS Page, Liquid Chromatography with Mass Spectroscopy

    SDS-PAGE analysis of B. pumilus spore protein extracts. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 3, BG-B79; 4, 8A4; 5, 8A6; 6, 14A1. A similar amount of protein was loaded into each 12% SDS-PAGE well. The rectangles (∼1 cm) were excised, digested in the gel with trypsin, and sequenced. The arrowheads indicate the sporulation-related manganese catalase (YjqC). BPUM_1305 was identified in all of the gel extracts tested.

    Journal: Applied and Environmental Microbiology

    Article Title: Protection of Bacillus pumilus Spores by Catalases

    doi: 10.1128/AEM.01211-12

    Figure Lengend Snippet: SDS-PAGE analysis of B. pumilus spore protein extracts. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 3, BG-B79; 4, 8A4; 5, 8A6; 6, 14A1. A similar amount of protein was loaded into each 12% SDS-PAGE well. The rectangles (∼1 cm) were excised, digested in the gel with trypsin, and sequenced. The arrowheads indicate the sporulation-related manganese catalase (YjqC). BPUM_1305 was identified in all of the gel extracts tested.

    Article Snippet: B. pumilus SAFR-032 and ATCC 7061 spore protein extracts were analyzed by SDS-PAGE.

    Techniques: SDS Page

    Manganese catalases identified by SDS-PAGE and LC-MS/MS in B.  pumilus  spores

    Journal: Applied and Environmental Microbiology

    Article Title: Protection of Bacillus pumilus Spores by Catalases

    doi: 10.1128/AEM.01211-12

    Figure Lengend Snippet: Manganese catalases identified by SDS-PAGE and LC-MS/MS in B. pumilus spores

    Article Snippet: B. pumilus SAFR-032 and ATCC 7061 spore protein extracts were analyzed by SDS-PAGE.

    Techniques:

    Catalase specific activities detected in B.  pumilus  spore suspensions

    Journal: Applied and Environmental Microbiology

    Article Title: Protection of Bacillus pumilus Spores by Catalases

    doi: 10.1128/AEM.01211-12

    Figure Lengend Snippet: Catalase specific activities detected in B. pumilus spore suspensions

    Article Snippet: B. pumilus SAFR-032 and ATCC 7061 spore protein extracts were analyzed by SDS-PAGE.

    Techniques: Activity Assay

    SDS-PAGE analysis of proteins extracted from intact and decoated B. pumilus spores. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 1A and 2A, intact spore protein extracts; 1B and 2B, decoated spore protein extracts; 1C and 2C, coat protein extracts. Lanes 1A and 1B were cut into ∼1.5-mm slices from the bottom to the top for protein identification by LC-MS/MS. A similar amount of protein (∼10 μg) was loaded into each well. The black arrowheads indicate a sporulation-related manganese catalase (YjqC). The white arrowheads indicate the second manganese catalase (BPUM_1305). The numbers indicate the excised PAGE bands.

    Journal: Applied and Environmental Microbiology

    Article Title: Protection of Bacillus pumilus Spores by Catalases

    doi: 10.1128/AEM.01211-12

    Figure Lengend Snippet: SDS-PAGE analysis of proteins extracted from intact and decoated B. pumilus spores. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 1A and 2A, intact spore protein extracts; 1B and 2B, decoated spore protein extracts; 1C and 2C, coat protein extracts. Lanes 1A and 1B were cut into ∼1.5-mm slices from the bottom to the top for protein identification by LC-MS/MS. A similar amount of protein (∼10 μg) was loaded into each well. The black arrowheads indicate a sporulation-related manganese catalase (YjqC). The white arrowheads indicate the second manganese catalase (BPUM_1305). The numbers indicate the excised PAGE bands.

    Article Snippet: Although the initial catalase activity in ATCC 7061 spores was higher, it was less stable over time than the SAFR-032 enzyme.

    Techniques: SDS Page, Liquid Chromatography with Mass Spectroscopy

    SDS-PAGE analysis of B. pumilus spore protein extracts. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 3, BG-B79; 4, 8A4; 5, 8A6; 6, 14A1. A similar amount of protein was loaded into each 12% SDS-PAGE well. The rectangles (∼1 cm) were excised, digested in the gel with trypsin, and sequenced. The arrowheads indicate the sporulation-related manganese catalase (YjqC). BPUM_1305 was identified in all of the gel extracts tested.

    Journal: Applied and Environmental Microbiology

    Article Title: Protection of Bacillus pumilus Spores by Catalases

    doi: 10.1128/AEM.01211-12

    Figure Lengend Snippet: SDS-PAGE analysis of B. pumilus spore protein extracts. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 3, BG-B79; 4, 8A4; 5, 8A6; 6, 14A1. A similar amount of protein was loaded into each 12% SDS-PAGE well. The rectangles (∼1 cm) were excised, digested in the gel with trypsin, and sequenced. The arrowheads indicate the sporulation-related manganese catalase (YjqC). BPUM_1305 was identified in all of the gel extracts tested.

    Article Snippet: Although the initial catalase activity in ATCC 7061 spores was higher, it was less stable over time than the SAFR-032 enzyme.

    Techniques: SDS Page

     Catalase  specific activities detected in B. pumilus spore suspensions

    Journal: Applied and Environmental Microbiology

    Article Title: Protection of Bacillus pumilus Spores by Catalases

    doi: 10.1128/AEM.01211-12

    Figure Lengend Snippet: Catalase specific activities detected in B. pumilus spore suspensions

    Article Snippet: Although the initial catalase activity in ATCC 7061 spores was higher, it was less stable over time than the SAFR-032 enzyme.

    Techniques: Activity Assay

    SDS-PAGE analysis of proteins extracted from intact and decoated B. pumilus spores. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 1A and 2A, intact spore protein extracts; 1B and 2B, decoated spore protein extracts; 1C and 2C, coat protein extracts. Lanes 1A and 1B were cut into ∼1.5-mm slices from the bottom to the top for protein identification by LC-MS/MS. A similar amount of protein (∼10 μg) was loaded into each well. The black arrowheads indicate a sporulation-related manganese catalase (YjqC). The white arrowheads indicate the second manganese catalase (BPUM_1305). The numbers indicate the excised PAGE bands.

    Journal: Applied and Environmental Microbiology

    Article Title: Protection of Bacillus pumilus Spores by Catalases

    doi: 10.1128/AEM.01211-12

    Figure Lengend Snippet: SDS-PAGE analysis of proteins extracted from intact and decoated B. pumilus spores. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 1A and 2A, intact spore protein extracts; 1B and 2B, decoated spore protein extracts; 1C and 2C, coat protein extracts. Lanes 1A and 1B were cut into ∼1.5-mm slices from the bottom to the top for protein identification by LC-MS/MS. A similar amount of protein (∼10 μg) was loaded into each well. The black arrowheads indicate a sporulation-related manganese catalase (YjqC). The white arrowheads indicate the second manganese catalase (BPUM_1305). The numbers indicate the excised PAGE bands.

    Article Snippet: Catalase activity optimization was performed at various pHs and temperatures for SAFR-032 and ATCC 7061 spore suspensions (data not shown).

    Techniques: SDS Page, Liquid Chromatography with Mass Spectroscopy

    SDS-PAGE analysis of B. pumilus spore protein extracts. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 3, BG-B79; 4, 8A4; 5, 8A6; 6, 14A1. A similar amount of protein was loaded into each 12% SDS-PAGE well. The rectangles (∼1 cm) were excised, digested in the gel with trypsin, and sequenced. The arrowheads indicate the sporulation-related manganese catalase (YjqC). BPUM_1305 was identified in all of the gel extracts tested.

    Journal: Applied and Environmental Microbiology

    Article Title: Protection of Bacillus pumilus Spores by Catalases

    doi: 10.1128/AEM.01211-12

    Figure Lengend Snippet: SDS-PAGE analysis of B. pumilus spore protein extracts. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 3, BG-B79; 4, 8A4; 5, 8A6; 6, 14A1. A similar amount of protein was loaded into each 12% SDS-PAGE well. The rectangles (∼1 cm) were excised, digested in the gel with trypsin, and sequenced. The arrowheads indicate the sporulation-related manganese catalase (YjqC). BPUM_1305 was identified in all of the gel extracts tested.

    Article Snippet: Catalase activity optimization was performed at various pHs and temperatures for SAFR-032 and ATCC 7061 spore suspensions (data not shown).

    Techniques: SDS Page

    Catalase specific activities detected in B. pumilus  spore suspensions

    Journal: Applied and Environmental Microbiology

    Article Title: Protection of Bacillus pumilus Spores by Catalases

    doi: 10.1128/AEM.01211-12

    Figure Lengend Snippet: Catalase specific activities detected in B. pumilus spore suspensions

    Article Snippet: Catalase activity optimization was performed at various pHs and temperatures for SAFR-032 and ATCC 7061 spore suspensions (data not shown).

    Techniques: Activity Assay