bacillus halodurans c 125  (ATCC)


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    ATCC bacillus halodurans c 125
    Methods for improving transformation of  Bacillus halodurans  C-125 (a) Protoplasts recovered on succinate nutrient agar (SNA, left) yield larger, more robust colonies than those on modified DM-3 medium (right). Colonies also appear two to three days sooner on SNA compared to modified DM-3 (data not shown). (b) Schematic of the Plasmid Artificial Modification (PAM) system for  B. halodurans  C-125. The three genes shown were inserted into the multiple cloning site of pBAD33 in-frame with each other and with an introduced ribosome binding site to ensure translation of the downstream genes. BH4003 and BH4004 were inserted as a unit, and their orientation reflects the native direction of transcription. (c) PAM treatment results in a 10-fold increase of colony-forming units (CFUs) for pMK4 (a ~5600 bp shuttle vector) and a 1000-fold increase for pHCMC05 (a ~8300 bp inducible expression vector). Error bars show the standard error of three independent transformations with 500 ng of plasmid. Both pMK4 and pHCMC05 were acquired from the  Bacillus  Genetic Stock Center.
    Bacillus Halodurans C 125, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bacillus halodurans c 125/product/ATCC
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bacillus halodurans c 125 - by Bioz Stars, 2022-05
    93/100 stars

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    1) Product Images from "Improved Genetic Transformation Methods for the Model Alkaliphile Bacillus halodurans C-125"

    Article Title: Improved Genetic Transformation Methods for the Model Alkaliphile Bacillus halodurans C-125

    Journal: Letters in applied microbiology

    doi: 10.1111/j.1472-765X.2011.03017.x

    Methods for improving transformation of  Bacillus halodurans  C-125 (a) Protoplasts recovered on succinate nutrient agar (SNA, left) yield larger, more robust colonies than those on modified DM-3 medium (right). Colonies also appear two to three days sooner on SNA compared to modified DM-3 (data not shown). (b) Schematic of the Plasmid Artificial Modification (PAM) system for  B. halodurans  C-125. The three genes shown were inserted into the multiple cloning site of pBAD33 in-frame with each other and with an introduced ribosome binding site to ensure translation of the downstream genes. BH4003 and BH4004 were inserted as a unit, and their orientation reflects the native direction of transcription. (c) PAM treatment results in a 10-fold increase of colony-forming units (CFUs) for pMK4 (a ~5600 bp shuttle vector) and a 1000-fold increase for pHCMC05 (a ~8300 bp inducible expression vector). Error bars show the standard error of three independent transformations with 500 ng of plasmid. Both pMK4 and pHCMC05 were acquired from the  Bacillus  Genetic Stock Center.
    Figure Legend Snippet: Methods for improving transformation of Bacillus halodurans C-125 (a) Protoplasts recovered on succinate nutrient agar (SNA, left) yield larger, more robust colonies than those on modified DM-3 medium (right). Colonies also appear two to three days sooner on SNA compared to modified DM-3 (data not shown). (b) Schematic of the Plasmid Artificial Modification (PAM) system for B. halodurans C-125. The three genes shown were inserted into the multiple cloning site of pBAD33 in-frame with each other and with an introduced ribosome binding site to ensure translation of the downstream genes. BH4003 and BH4004 were inserted as a unit, and their orientation reflects the native direction of transcription. (c) PAM treatment results in a 10-fold increase of colony-forming units (CFUs) for pMK4 (a ~5600 bp shuttle vector) and a 1000-fold increase for pHCMC05 (a ~8300 bp inducible expression vector). Error bars show the standard error of three independent transformations with 500 ng of plasmid. Both pMK4 and pHCMC05 were acquired from the Bacillus Genetic Stock Center.

    Techniques Used: Transformation Assay, Modification, Plasmid Preparation, Clone Assay, Binding Assay, Expressing

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    ATCC bacillus halodurans c 125
    Methods for improving transformation of  Bacillus halodurans  C-125 (a) Protoplasts recovered on succinate nutrient agar (SNA, left) yield larger, more robust colonies than those on modified DM-3 medium (right). Colonies also appear two to three days sooner on SNA compared to modified DM-3 (data not shown). (b) Schematic of the Plasmid Artificial Modification (PAM) system for  B. halodurans  C-125. The three genes shown were inserted into the multiple cloning site of pBAD33 in-frame with each other and with an introduced ribosome binding site to ensure translation of the downstream genes. BH4003 and BH4004 were inserted as a unit, and their orientation reflects the native direction of transcription. (c) PAM treatment results in a 10-fold increase of colony-forming units (CFUs) for pMK4 (a ~5600 bp shuttle vector) and a 1000-fold increase for pHCMC05 (a ~8300 bp inducible expression vector). Error bars show the standard error of three independent transformations with 500 ng of plasmid. Both pMK4 and pHCMC05 were acquired from the  Bacillus  Genetic Stock Center.
    Bacillus Halodurans C 125, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bacillus halodurans c 125/product/ATCC
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bacillus halodurans c 125 - by Bioz Stars, 2022-05
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    Methods for improving transformation of  Bacillus halodurans  C-125 (a) Protoplasts recovered on succinate nutrient agar (SNA, left) yield larger, more robust colonies than those on modified DM-3 medium (right). Colonies also appear two to three days sooner on SNA compared to modified DM-3 (data not shown). (b) Schematic of the Plasmid Artificial Modification (PAM) system for  B. halodurans  C-125. The three genes shown were inserted into the multiple cloning site of pBAD33 in-frame with each other and with an introduced ribosome binding site to ensure translation of the downstream genes. BH4003 and BH4004 were inserted as a unit, and their orientation reflects the native direction of transcription. (c) PAM treatment results in a 10-fold increase of colony-forming units (CFUs) for pMK4 (a ~5600 bp shuttle vector) and a 1000-fold increase for pHCMC05 (a ~8300 bp inducible expression vector). Error bars show the standard error of three independent transformations with 500 ng of plasmid. Both pMK4 and pHCMC05 were acquired from the  Bacillus  Genetic Stock Center.

    Journal: Letters in applied microbiology

    Article Title: Improved Genetic Transformation Methods for the Model Alkaliphile Bacillus halodurans C-125

    doi: 10.1111/j.1472-765X.2011.03017.x

    Figure Lengend Snippet: Methods for improving transformation of Bacillus halodurans C-125 (a) Protoplasts recovered on succinate nutrient agar (SNA, left) yield larger, more robust colonies than those on modified DM-3 medium (right). Colonies also appear two to three days sooner on SNA compared to modified DM-3 (data not shown). (b) Schematic of the Plasmid Artificial Modification (PAM) system for B. halodurans C-125. The three genes shown were inserted into the multiple cloning site of pBAD33 in-frame with each other and with an introduced ribosome binding site to ensure translation of the downstream genes. BH4003 and BH4004 were inserted as a unit, and their orientation reflects the native direction of transcription. (c) PAM treatment results in a 10-fold increase of colony-forming units (CFUs) for pMK4 (a ~5600 bp shuttle vector) and a 1000-fold increase for pHCMC05 (a ~8300 bp inducible expression vector). Error bars show the standard error of three independent transformations with 500 ng of plasmid. Both pMK4 and pHCMC05 were acquired from the Bacillus Genetic Stock Center.

    Article Snippet: One such bacterium is Bacillus halodurans C-125 (ATCC# BAA-125), a model alkaliphile and source of a several enzymes of industrial importance ( ).

    Techniques: Transformation Assay, Modification, Plasmid Preparation, Clone Assay, Binding Assay, Expressing