Bacillus Halodurans C 125, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bacillus halodurans c 125/product/ATCC
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
1) Product Images from "Improved Genetic Transformation Methods for the Model Alkaliphile Bacillus halodurans C-125"
Article Title: Improved Genetic Transformation Methods for the Model Alkaliphile Bacillus halodurans C-125
Journal: Letters in applied microbiology
Figure Legend Snippet: Methods for improving transformation of Bacillus halodurans C-125 (a) Protoplasts recovered on succinate nutrient agar (SNA, left) yield larger, more robust colonies than those on modified DM-3 medium (right). Colonies also appear two to three days sooner on SNA compared to modified DM-3 (data not shown). (b) Schematic of the Plasmid Artificial Modification (PAM) system for B. halodurans C-125. The three genes shown were inserted into the multiple cloning site of pBAD33 in-frame with each other and with an introduced ribosome binding site to ensure translation of the downstream genes. BH4003 and BH4004 were inserted as a unit, and their orientation reflects the native direction of transcription. (c) PAM treatment results in a 10-fold increase of colony-forming units (CFUs) for pMK4 (a ~5600 bp shuttle vector) and a 1000-fold increase for pHCMC05 (a ~8300 bp inducible expression vector). Error bars show the standard error of three independent transformations with 500 ng of plasmid. Both pMK4 and pHCMC05 were acquired from the Bacillus Genetic Stock Center.
Techniques Used: Transformation Assay, Modification, Plasmid Preparation, Clone Assay, Binding Assay, Expressing