baby hamster kidney fibroblasts bhk 21 cells  (ATCC)


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    Name:
    BHK 21 C 13
    Description:

    Catalog Number:
    ccl-10
    Price:
    None
    Applications:
    The World Organization for Animal Health (OIE) uses these cells for routine diagnosis of rabies.  This cell line has been used as a host for transformation with expression vectors containing selectable and amplifiable marker DNAs (e.g., Factor VIII, see ATCC CRL-8544).
    Host:
    Mesocricetus auratus, hamster, Syrian golden
    Cell Type:
    fibroblast
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    Structured Review

    ATCC baby hamster kidney fibroblasts bhk 21 cells
    IBV S1 inhibits rcMBLs antiviral activity . <t>BHK-21</t> cells were infected with IBV Beaudette at an MOI of 0.1 in the presence or absence of 10 μg/ml rcMBL and 50 μg/ml M41 S1. Infection was determined by immunofluorescence using an antibody directed against the IBV nucleocapsid protein at 8 h post-infection.

    https://www.bioz.com/result/baby hamster kidney fibroblasts bhk 21 cells/product/ATCC
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    baby hamster kidney fibroblasts bhk 21 cells - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Chicken mannose binding lectin has antiviral activity towards infectious bronchitis virus"

    Article Title: Chicken mannose binding lectin has antiviral activity towards infectious bronchitis virus

    Journal: Virology

    doi: 10.1016/j.virol.2017.06.028

    IBV S1 inhibits rcMBLs antiviral activity . BHK-21 cells were infected with IBV Beaudette at an MOI of 0.1 in the presence or absence of 10 μg/ml rcMBL and 50 μg/ml M41 S1. Infection was determined by immunofluorescence using an antibody directed against the IBV nucleocapsid protein at 8 h post-infection.
    Figure Legend Snippet: IBV S1 inhibits rcMBLs antiviral activity . BHK-21 cells were infected with IBV Beaudette at an MOI of 0.1 in the presence or absence of 10 μg/ml rcMBL and 50 μg/ml M41 S1. Infection was determined by immunofluorescence using an antibody directed against the IBV nucleocapsid protein at 8 h post-infection.

    Techniques Used: Activity Assay, Infection, Immunofluorescence

    rcMBL inhibits IBV-Beaudette infection of BHK 21 cells in a concentration dependent manner . BHK-21 cells were inoculated after pre-incubation of IBV-Beaudette (MOI of 0.1) with various concentrations of rcMBL. A) Infection was determined by immunofluorescence using an antibody directed against the IBV nucleocapsid protein at 8 h post-infection. Bar: 50 µm. B) Infection was determined by quantitative PCR: the IBV viral genome expression relative to GAPDH gene expression was determined and expressed relative to the no-rcMBL control. C) The toxic effects of rcMBL on BHK-21 cells was determined by the WST-1 assay. Asterisks indicate statistical significant difference compared to the no rcMBL control. Shown are mean ± SEM of three independent experiments.
    Figure Legend Snippet: rcMBL inhibits IBV-Beaudette infection of BHK 21 cells in a concentration dependent manner . BHK-21 cells were inoculated after pre-incubation of IBV-Beaudette (MOI of 0.1) with various concentrations of rcMBL. A) Infection was determined by immunofluorescence using an antibody directed against the IBV nucleocapsid protein at 8 h post-infection. Bar: 50 µm. B) Infection was determined by quantitative PCR: the IBV viral genome expression relative to GAPDH gene expression was determined and expressed relative to the no-rcMBL control. C) The toxic effects of rcMBL on BHK-21 cells was determined by the WST-1 assay. Asterisks indicate statistical significant difference compared to the no rcMBL control. Shown are mean ± SEM of three independent experiments.

    Techniques Used: Infection, Concentration Assay, Incubation, Immunofluorescence, Real-time Polymerase Chain Reaction, Expressing, WST-1 Assay

    Related Articles

    Electroporation:

    Article Title: Mutating Conserved Cysteines in the Alphavirus E2 Glycoprotein Causes Virus-Specific Assembly Defects
    Article Snippet: .. Electroporation of BHK-21 cells was performed as previously described ( ). ..

    In Vitro:

    Article Title: Mutating Conserved Cysteines in the Alphavirus E2 Glycoprotein Causes Virus-Specific Assembly Defects
    Article Snippet: .. BHK-21 cells were electroporated with in vitro -transcribed wild-type or mutant viral RNA, resuspended in BHK cell medium, and incubated at 37°C in the presence of 5% CO2 . ..

    Mutagenesis:

    Article Title: Mutating Conserved Cysteines in the Alphavirus E2 Glycoprotein Causes Virus-Specific Assembly Defects
    Article Snippet: .. BHK-21 cells were electroporated with in vitro -transcribed wild-type or mutant viral RNA, resuspended in BHK cell medium, and incubated at 37°C in the presence of 5% CO2 . ..

    Article Title: Murine Gammaherpesvirus 68 Lacking gp150 Shows Defective Virion Release but Establishes Normal Latency In Vivo
    Article Snippet: .. A similar phenotype was observed with the independent mutant (M7− STOP) and with growth in BHK-21 cells, as well as MEFs (Fig. ). ..

    Cell Culture:

    Article Title: A Novel Dengue Virus Inhibitor, BP13944, Discovered by High-Throughput Screening with Dengue Virus Replicon Cells Selects for Resistance in the Viral NS2B/NS3 Protease
    Article Snippet: .. BHK-21 cells (ATCC CCL-10) were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 4.5 g/liter glucose and 5% fetal bovine serum (FBS). .. The cells were cultured at 37°C in a 5% CO2 incubator.

    Incubation:

    Article Title: Mutating Conserved Cysteines in the Alphavirus E2 Glycoprotein Causes Virus-Specific Assembly Defects
    Article Snippet: .. BHK-21 cells were electroporated with in vitro -transcribed wild-type or mutant viral RNA, resuspended in BHK cell medium, and incubated at 37°C in the presence of 5% CO2 . ..

    other:

    Article Title: Mutating Conserved Cysteines in the Alphavirus E2 Glycoprotein Causes Virus-Specific Assembly Defects
    Article Snippet: BHK-21 cells were grown at 37°C in the presence of 5% CO2 .

    Article Title: Application of interferon modulators to overcome partial resistance of human ovarian cancers to VSV-GP oncolytic viral therapy
    Article Snippet: Cell lines BHK21 cells (American Type Culture Collection, Manassas, VA) were maintained in Glasgow minimum essential medium (GMEM) (Gibco, Carlsbad, CA) supplemented with 10% fetal calf serum (FCS; PAA Laboratories, Cölbe, Germany), 5% tryptose phosphate broth (Gibco, Carlsbad, CA), 100 units/ml penicillin (Gibco), and 0.1 mg/ml streptomycin (Gibco).

    Modification:

    Article Title: Mutations in the DI-DII Linker of Human Parainfluenza Virus Type 3 Fusion Protein Result in Diminished Fusion Activity
    Article Snippet: .. Cells and viruses BHK-21 cells, obtained from the American Type Culture Collection, were propagated in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco) supplemented with 1% glutamine, 10% fetal calf serum (FCS) (Gibco), and 1% penicillin-streptomycin (Invitrogen). .. Wild-type (wt) vaccinia virus was used to quantify cell fusion, while recombinant vaccinia virus vTF7-3, a generous gift from Dr. Bernard Moss, was maintained in BHK-21 cells to provide T7 RNA polymerase in the vaccinia-T7 RNA polymerase expression system [ ].

    Article Title: Glycoprotein B Cleavage Is Important for Murid Herpesvirus 4 To Infect Myeloid Cells
    Article Snippet: .. BHK-21, NMuMG, NS0, and RAW 264.7 cells (all from ATCC) were grown in Dulbecco's modified Eagle's medium (DMEM; high glucose; PAA Laboratories) with 2 mM l -glutamine (PAA Laboratories), 100 U/ml penicillin and 100 μg/ml streptomycin (PAA Laboratories), and 10% fetal bovine serum (FBS; PAA Laboratories or Amimed) (complete medium). .. Peritoneal cavity cells were isolated from BALB/c mice by peritoneal lavage and cultivated in RPMI 1640 (PAA Laboratories) with 10% heat-inactivated (56°C, 30 min) FBS (PAA Laboratories or Amimed), 2 mM l -glutamine (PAA Laboratories), and 50 U/ml penicillin and 50 μg/ml streptomycin (PAA Laboratories).

    Article Title: A Novel Dengue Virus Inhibitor, BP13944, Discovered by High-Throughput Screening with Dengue Virus Replicon Cells Selects for Resistance in the Viral NS2B/NS3 Protease
    Article Snippet: .. BHK-21 cells (ATCC CCL-10) were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 4.5 g/liter glucose and 5% fetal bovine serum (FBS). .. The cells were cultured at 37°C in a 5% CO2 incubator.

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    ATCC bhk 21 cells
    Viral gene expression and DNA replication of the ORF45-null mutant. (A and B) <t>BHK-21</t> cells were infected with the wt or ORF45-null (45STOP) virus at an MOI of 500 genome copies per cell. Cellular RNAs were isolated at the indicated times postinfection
    Bhk 21 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bhk 21 cells/product/ATCC
    Average 99 stars, based on 69 article reviews
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    Viral gene expression and DNA replication of the ORF45-null mutant. (A and B) BHK-21 cells were infected with the wt or ORF45-null (45STOP) virus at an MOI of 500 genome copies per cell. Cellular RNAs were isolated at the indicated times postinfection

    Journal: Journal of Virology

    Article Title: Murine Gammaherpesvirus 68 Open Reading Frame 45 Plays an Essential Role during the Immediate-Early Phase of Viral Replication

    doi: 10.1128/JVI.79.8.5129-5141.2005

    Figure Lengend Snippet: Viral gene expression and DNA replication of the ORF45-null mutant. (A and B) BHK-21 cells were infected with the wt or ORF45-null (45STOP) virus at an MOI of 500 genome copies per cell. Cellular RNAs were isolated at the indicated times postinfection

    Article Snippet: The working wt MHV-68 virus stock was generated by infecting BHK-21 cells (a baby hamster kidney fibroblast cell line; ATCC CCL-10) at a multiplicity of infection (MOI) of 0.01 PFU per cell and harvesting them at 5 days postinfection (p.i.).

    Techniques: Expressing, Mutagenesis, Infection, Isolation

    Deficiency in and complementation of viral replication for the ORF45-null mutant. (A) BHK-21 cells were transfected with wt MHV-68(BAC) plus plasmid pEGFP-C1 (panels 1, 4, 1′, and 4′), 45STOP plus pEGFP-C1 (panels 2, 5, 2′, and

    Journal: Journal of Virology

    Article Title: Murine Gammaherpesvirus 68 Open Reading Frame 45 Plays an Essential Role during the Immediate-Early Phase of Viral Replication

    doi: 10.1128/JVI.79.8.5129-5141.2005

    Figure Lengend Snippet: Deficiency in and complementation of viral replication for the ORF45-null mutant. (A) BHK-21 cells were transfected with wt MHV-68(BAC) plus plasmid pEGFP-C1 (panels 1, 4, 1′, and 4′), 45STOP plus pEGFP-C1 (panels 2, 5, 2′, and

    Article Snippet: The working wt MHV-68 virus stock was generated by infecting BHK-21 cells (a baby hamster kidney fibroblast cell line; ATCC CCL-10) at a multiplicity of infection (MOI) of 0.01 PFU per cell and harvesting them at 5 days postinfection (p.i.).

    Techniques: Mutagenesis, Transfection, Plasmid Preparation

    Analysis of functional domain(s) of ORF45 in viral replication. (A) BHK-21 cells were mock transfected or cotransfected with the wt, ORF45-null (45STOP), or null-revertant (45STOP.R) BAC DNA plus an expression plasmid containing GFP or GFP fused to full-length

    Journal: Journal of Virology

    Article Title: Murine Gammaherpesvirus 68 Open Reading Frame 45 Plays an Essential Role during the Immediate-Early Phase of Viral Replication

    doi: 10.1128/JVI.79.8.5129-5141.2005

    Figure Lengend Snippet: Analysis of functional domain(s) of ORF45 in viral replication. (A) BHK-21 cells were mock transfected or cotransfected with the wt, ORF45-null (45STOP), or null-revertant (45STOP.R) BAC DNA plus an expression plasmid containing GFP or GFP fused to full-length

    Article Snippet: The working wt MHV-68 virus stock was generated by infecting BHK-21 cells (a baby hamster kidney fibroblast cell line; ATCC CCL-10) at a multiplicity of infection (MOI) of 0.01 PFU per cell and harvesting them at 5 days postinfection (p.i.).

    Techniques: Functional Assay, Transfection, BAC Assay, Expressing, Plasmid Preparation

    Susceptibility to gB-directed neutralization of WT, gL − and gBΔ2-30 virions. WT virions or those lacking gL (gL − ) or the N-terminal 30 residues of the mature gB (gBΔ2-30) were incubated with gB-specific mAbs as shown then added to BHK-21 cells. The cells were scored for virus infection by flow cytometric assay of viral eGFP after overnight incubation (37 °C) with 100 µg phosphonoacetic acid ml −1 . SC-9A5 and SC-9E8 neutralized the gL − and gBΔ2-30 mutants significantly better than WT ( P

    Journal: The Journal of General Virology

    Article Title: A mechanistic basis for potent, glycoprotein B-directed gammaherpesvirus neutralization

    doi: 10.1099/vir.0.032177-0

    Figure Lengend Snippet: Susceptibility to gB-directed neutralization of WT, gL − and gBΔ2-30 virions. WT virions or those lacking gL (gL − ) or the N-terminal 30 residues of the mature gB (gBΔ2-30) were incubated with gB-specific mAbs as shown then added to BHK-21 cells. The cells were scored for virus infection by flow cytometric assay of viral eGFP after overnight incubation (37 °C) with 100 µg phosphonoacetic acid ml −1 . SC-9A5 and SC-9E8 neutralized the gL − and gBΔ2-30 mutants significantly better than WT ( P

    Article Snippet: BHK-21 fibroblasts (American type culture collection CCL-10), NMuMG epithelial cells (CRL-1636), 293T cells (CRL-11268) and RAW-264 macrophages (TIB-71) were grown in Dulbecco’s modified Eagle’s medium with 2 mM glutamine, 100 U penicillin ml−1 , 100 µg streptomycin ml−1 (PAA Laboratories) and 10 % FCS (complete medium) (PAA Laboratories).

    Techniques: Neutralization, Incubation, Infection, Flow Cytometry

    (a) Virus neutralization by gB-specific mAbs SC-9A5 and SC-9E8. Bacterial artificial chromosome (BAC) + MuHV-4 (0.1 p.f.u. per cell) was incubated with gB-specific mAbs SC-9A5 (IgG 3 ), SC-9E8 (IgG 2a ), BN-1A7 (IgG 2a , non-neutralizing) or MG-2C10 (IgM, neutralizing) before being added to BHK-21 cells. After overnight incubation (37 °C) in the presence of 100 µg phosphonoacetic acid ml −1 to prevent further virus spread, eGFP + cells were enumerated by flow cytometry and are shown relative to untreated virus. Each point shows the mean± sem of two experiments. By chi-squared test comparing the proportions of eGFP + and eGFP − cells, mAbs SC-9A5 and SC-9E8 gave significantly less neutralization than mAb MG-2C10 at

    Journal: The Journal of General Virology

    Article Title: A mechanistic basis for potent, glycoprotein B-directed gammaherpesvirus neutralization

    doi: 10.1099/vir.0.032177-0

    Figure Lengend Snippet: (a) Virus neutralization by gB-specific mAbs SC-9A5 and SC-9E8. Bacterial artificial chromosome (BAC) + MuHV-4 (0.1 p.f.u. per cell) was incubated with gB-specific mAbs SC-9A5 (IgG 3 ), SC-9E8 (IgG 2a ), BN-1A7 (IgG 2a , non-neutralizing) or MG-2C10 (IgM, neutralizing) before being added to BHK-21 cells. After overnight incubation (37 °C) in the presence of 100 µg phosphonoacetic acid ml −1 to prevent further virus spread, eGFP + cells were enumerated by flow cytometry and are shown relative to untreated virus. Each point shows the mean± sem of two experiments. By chi-squared test comparing the proportions of eGFP + and eGFP − cells, mAbs SC-9A5 and SC-9E8 gave significantly less neutralization than mAb MG-2C10 at

    Article Snippet: BHK-21 fibroblasts (American type culture collection CCL-10), NMuMG epithelial cells (CRL-1636), 293T cells (CRL-11268) and RAW-264 macrophages (TIB-71) were grown in Dulbecco’s modified Eagle’s medium with 2 mM glutamine, 100 U penicillin ml−1 , 100 µg streptomycin ml−1 (PAA Laboratories) and 10 % FCS (complete medium) (PAA Laboratories).

    Techniques: Neutralization, BAC Assay, Incubation, Flow Cytometry, Cytometry

    (a) SC-9A5 and SC-9E8 inhibit infection without reducing cell binding. To assess virus binding (left panel), gM-eGFP + virions (3 p.f.u. per cell) were pre-incubated with mAbs, MuHV-4-immune serum or heparin (2 h, 37 °C) before binding to NMuMG cells (2 h, 4 °C). Unbound virions were removed by washing and the cells analysed immediately for eGFP fluorescence by flow cytometry. To assess virus entry (right panel), BAC + MuHV-4 (0.1 p.f.u. per cell) was pre-incubated as above then added to NMuMG cells, which were analysed for viral eGFP expression after overnight incubation (37 °C) with 100 µg phosphonoacetic acid ml −1 . The bars show mean± sem values from two independent experiments. (b) SC-9A5 and SC-9E8 neutralize both cell-bound and cell-free virions. To neutralize cell-free virions, BAC + MuHV-4 (0.3 p.f.u. per cell) was pre-incubated with mAbs or immune serum (2 h, 4 °C), then bound to BHK-21 cells (2 h, 4 °C). Unbound virions were removed by washing with PBS. To neutralize cell-bound virus, BHK-21 cells were incubated with BAC + MuHV-4 (0.3 p.f.u. per cell, 2 h, 4 °C), washed, incubated with mAbs or immune serum (2 h, 4 °C), then washed again. All cells were then incubated overnight (37 °C) with 100 µg phosphonoacetic acid ml −1 and analysed for viral eGFP expression by flow cytometry. For SC-9A5 and SC-9E8 ID 50 values for either method differed

    Journal: The Journal of General Virology

    Article Title: A mechanistic basis for potent, glycoprotein B-directed gammaherpesvirus neutralization

    doi: 10.1099/vir.0.032177-0

    Figure Lengend Snippet: (a) SC-9A5 and SC-9E8 inhibit infection without reducing cell binding. To assess virus binding (left panel), gM-eGFP + virions (3 p.f.u. per cell) were pre-incubated with mAbs, MuHV-4-immune serum or heparin (2 h, 37 °C) before binding to NMuMG cells (2 h, 4 °C). Unbound virions were removed by washing and the cells analysed immediately for eGFP fluorescence by flow cytometry. To assess virus entry (right panel), BAC + MuHV-4 (0.1 p.f.u. per cell) was pre-incubated as above then added to NMuMG cells, which were analysed for viral eGFP expression after overnight incubation (37 °C) with 100 µg phosphonoacetic acid ml −1 . The bars show mean± sem values from two independent experiments. (b) SC-9A5 and SC-9E8 neutralize both cell-bound and cell-free virions. To neutralize cell-free virions, BAC + MuHV-4 (0.3 p.f.u. per cell) was pre-incubated with mAbs or immune serum (2 h, 4 °C), then bound to BHK-21 cells (2 h, 4 °C). Unbound virions were removed by washing with PBS. To neutralize cell-bound virus, BHK-21 cells were incubated with BAC + MuHV-4 (0.3 p.f.u. per cell, 2 h, 4 °C), washed, incubated with mAbs or immune serum (2 h, 4 °C), then washed again. All cells were then incubated overnight (37 °C) with 100 µg phosphonoacetic acid ml −1 and analysed for viral eGFP expression by flow cytometry. For SC-9A5 and SC-9E8 ID 50 values for either method differed

    Article Snippet: BHK-21 fibroblasts (American type culture collection CCL-10), NMuMG epithelial cells (CRL-1636), 293T cells (CRL-11268) and RAW-264 macrophages (TIB-71) were grown in Dulbecco’s modified Eagle’s medium with 2 mM glutamine, 100 U penicillin ml−1 , 100 µg streptomycin ml−1 (PAA Laboratories) and 10 % FCS (complete medium) (PAA Laboratories).

    Techniques: Infection, Binding Assay, Incubation, Fluorescence, Flow Cytometry, Cytometry, BAC Assay, Expressing

    In vitro neutralization of MuHV-4 virions by mAbs. MuHV-4 virions (100 p.f.u.) were incubated with mAbs and then plaque-assayed on BHK-21 cells. The highest amounts of each antibody used for neutralization correspond to the amounts given per mouse. Small changes in infectivity (

    Journal: The Journal of General Virology

    Article Title: Antibody limits in vivo murid herpesvirus-4 replication by IgG Fc receptor-dependent functions

    doi: 10.1099/vir.0.014266-0

    Figure Lengend Snippet: In vitro neutralization of MuHV-4 virions by mAbs. MuHV-4 virions (100 p.f.u.) were incubated with mAbs and then plaque-assayed on BHK-21 cells. The highest amounts of each antibody used for neutralization correspond to the amounts given per mouse. Small changes in infectivity (

    Article Snippet: This manifests as virions from NMuMG epithelial cells resisting recognition by MG-2C10 unless O -glycans are removed, whereas virions from BHK-21 fibroblasts are recognized with or without deglycosylation. (Cells of epithelial origin presumably add more extensive, mucin-like O -glycans.)

    Techniques: In Vitro, Neutralization, Incubation, Infection

    Comparison of biological characteristics between recovered GZ01 and the parental strain. (A) Identification of the KpnI-marker in recovered ZIKV. Viral RNA was isolated from supernatants of GZ01 and rGZ01 and ZIKV cDNA fragments were amplified by RT-PCR and digested by the endonuclease KpnI. The KpnI-digested PCR products [KpnI (+)] and undigested controls [KpnI (−)] were analyzed by electrophoresis in a 1.5% agarose–TAE gel. Lane M, DNA marker DL 2,000. (B) Plaque morphologies of rGZ01 and parental GZ01. Mock, uninfected BHK-21 cells. (C) BHK-21 cells were infected with an MOI of 0.1 of rGZ01 and GZ01, respectively, and viral E protein expression was monitored by IFA at different time points after infection. Uninfected BHK-21 cells were subjected to IFA and the results were shown in parallel (mock). (D and E) Comparison of the growth kinetics of rGZ01 and GZ01. (D) Infectious viruses in the supernatants of MOI 0.1-infected BHK-21 cells were determined by plaque assay. (E) C6/36 cells were infected with rGZ01 and GZ01 at an MOI of 0.1, culture supernatants were collected at different time points postinfection, and vRNA levels were determined by qRT-PCR. (F) Neurovirulence of rGZ01 and GZ01 in neonatal BALB/c mice. One-day-old BALB/c suckling mice ( n = 7) were incubated with 10 PFU of rGZ01 or GZ01, respectively, and the survival statistics were monitored daily.

    Journal: Journal of Virology

    Article Title: Characterization of cis-Acting RNA Elements of Zika Virus by Using a Self-Splicing Ribozyme-Dependent Infectious Clone

    doi: 10.1128/JVI.00484-17

    Figure Lengend Snippet: Comparison of biological characteristics between recovered GZ01 and the parental strain. (A) Identification of the KpnI-marker in recovered ZIKV. Viral RNA was isolated from supernatants of GZ01 and rGZ01 and ZIKV cDNA fragments were amplified by RT-PCR and digested by the endonuclease KpnI. The KpnI-digested PCR products [KpnI (+)] and undigested controls [KpnI (−)] were analyzed by electrophoresis in a 1.5% agarose–TAE gel. Lane M, DNA marker DL 2,000. (B) Plaque morphologies of rGZ01 and parental GZ01. Mock, uninfected BHK-21 cells. (C) BHK-21 cells were infected with an MOI of 0.1 of rGZ01 and GZ01, respectively, and viral E protein expression was monitored by IFA at different time points after infection. Uninfected BHK-21 cells were subjected to IFA and the results were shown in parallel (mock). (D and E) Comparison of the growth kinetics of rGZ01 and GZ01. (D) Infectious viruses in the supernatants of MOI 0.1-infected BHK-21 cells were determined by plaque assay. (E) C6/36 cells were infected with rGZ01 and GZ01 at an MOI of 0.1, culture supernatants were collected at different time points postinfection, and vRNA levels were determined by qRT-PCR. (F) Neurovirulence of rGZ01 and GZ01 in neonatal BALB/c mice. One-day-old BALB/c suckling mice ( n = 7) were incubated with 10 PFU of rGZ01 or GZ01, respectively, and the survival statistics were monitored daily.

    Article Snippet: The baby hamster kidney fibroblast cell line BHK-21 (ATCC CCL10) was cultured in Dulbecco modified Eagle medium (DMEM; Gibco, Thermo Fisher Scientific) containing 5% fetal bovine serum (FBS; Biowest) and 1% penicillin-streptomycin (PS; Thermo Fisher Scientific).

    Techniques: Marker, Isolation, Amplification, Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Electrophoresis, Infection, Expressing, Immunofluorescence, Plaque Assay, Quantitative RT-PCR, Mouse Assay, Incubation