babb  (Millipore)


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  • 99
    Name:
    Alcohol
    Description:
    Pharmaceutical secondary standards for application in quality control provide pharma laboratories and manufacturers with a convenient and cost effective alternative to the preparation of in house working standards
    Catalog Number:
    phr1373
    Price:
    None
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    Structured Review

    Millipore babb
    Alcohol
    Pharmaceutical secondary standards for application in quality control provide pharma laboratories and manufacturers with a convenient and cost effective alternative to the preparation of in house working standards
    https://www.bioz.com/result/babb/product/Millipore
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    babb - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Chemical Clearing and Dehydration of GFP Expressing Mouse Brains"

    Article Title: Chemical Clearing and Dehydration of GFP Expressing Mouse Brains

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0033916

    Removal of peroxides from THF and DBE. A : Apparatus for peroxide cleaning of THF 1 : Dropping funnel with pressure compensation. 2 : Chromatography column filled with basic activated aluminum oxide activity grade Brockman 1 3 : Two necked round bottom flask 4 : Drying tube filled with calcium chloride. B : Apparatus for peroxide removal in DBE and BABB 1 : Filter unit with filter plate (16–40 µm pore size) 2 : Vacuum tight filtering flask.
    Figure Legend Snippet: Removal of peroxides from THF and DBE. A : Apparatus for peroxide cleaning of THF 1 : Dropping funnel with pressure compensation. 2 : Chromatography column filled with basic activated aluminum oxide activity grade Brockman 1 3 : Two necked round bottom flask 4 : Drying tube filled with calcium chloride. B : Apparatus for peroxide removal in DBE and BABB 1 : Filter unit with filter plate (16–40 µm pore size) 2 : Vacuum tight filtering flask.

    Techniques Used: Chromatography, Activity Assay

    Comparison of THF/DBE clearing with ethanol/BABB. A–B : Left and right brain hemispheres from two mice (postnatal day 101). The left hemispheres of each animal were dehydrated with ethanol, or THF. The corresponding right hemispheres were dehydrated with ethanol as a control. Afterwards, the hemispheres were rendered transparent with BABB (A1 and B1. C : Left and right brain hemisphere of an exemplary mouse (postnatal day 101). The left hemisphere was dehydrated with THF and cleared with DBE. The right hemisphere was dehydrated with ethanol and then cleared with BABB as a control (C1). From all hemispheres 3D reconstruction were performed by UM (A2–C2, Olympus objective XLFluar 4×, N.A. 0.28). As evident from A–C, THF-dehydration provides an improved fluorescence signal (B2), and considerably reduced background fluorescence (B2), while DBE-clearing provides considerably better tissue transparency (C1) and strikingly better fluorescence preservation (C2). The black bars below A2–C2 represent intensity histograms of the respective image stacks used for 3D reconstruction. Length of scale bars in Figures A1–C1 is 2 mm. Scales are identical for all histograms A2–C2 and thus are only shown in the upper two histograms.
    Figure Legend Snippet: Comparison of THF/DBE clearing with ethanol/BABB. A–B : Left and right brain hemispheres from two mice (postnatal day 101). The left hemispheres of each animal were dehydrated with ethanol, or THF. The corresponding right hemispheres were dehydrated with ethanol as a control. Afterwards, the hemispheres were rendered transparent with BABB (A1 and B1. C : Left and right brain hemisphere of an exemplary mouse (postnatal day 101). The left hemisphere was dehydrated with THF and cleared with DBE. The right hemisphere was dehydrated with ethanol and then cleared with BABB as a control (C1). From all hemispheres 3D reconstruction were performed by UM (A2–C2, Olympus objective XLFluar 4×, N.A. 0.28). As evident from A–C, THF-dehydration provides an improved fluorescence signal (B2), and considerably reduced background fluorescence (B2), while DBE-clearing provides considerably better tissue transparency (C1) and strikingly better fluorescence preservation (C2). The black bars below A2–C2 represent intensity histograms of the respective image stacks used for 3D reconstruction. Length of scale bars in Figures A1–C1 is 2 mm. Scales are identical for all histograms A2–C2 and thus are only shown in the upper two histograms.

    Techniques Used: Mouse Assay, Fluorescence, Preserving

    UM-reconstructions of isolated hippocampi of a thy-1 EGFP-M mouse. DBE clearing provides improved fluorescence and visibility of details. A : left hippocampus dehydrated with THF and cleared with BABB. B : corresponding right hippocampus dehydrated with THF and cleared with DBE. A1–B1 : Olympus objective XLFluar 4×, N.A. 0.28, scale bar 100 µm. ( A2–B2 ) 4× Olympus objective XLFluar 4×, N.A. 0.28, scale bar 20 µm. A3–B3 : 4× Olympus objective XLFluar 4×, N.A. 0.28, scale bar 10 µm. CA1 : cornu ammonis region one. gl : granular cell layer py : pyramidal cells.
    Figure Legend Snippet: UM-reconstructions of isolated hippocampi of a thy-1 EGFP-M mouse. DBE clearing provides improved fluorescence and visibility of details. A : left hippocampus dehydrated with THF and cleared with BABB. B : corresponding right hippocampus dehydrated with THF and cleared with DBE. A1–B1 : Olympus objective XLFluar 4×, N.A. 0.28, scale bar 100 µm. ( A2–B2 ) 4× Olympus objective XLFluar 4×, N.A. 0.28, scale bar 20 µm. A3–B3 : 4× Olympus objective XLFluar 4×, N.A. 0.28, scale bar 10 µm. CA1 : cornu ammonis region one. gl : granular cell layer py : pyramidal cells.

    Techniques Used: Isolation, Fluorescence

    Related Articles

    Flow Cytometry:

    Article Title: Increasing the Sensitivity of ELISA using Multiplexed Electrokinetic Concentrator
    Article Snippet: .. A 50 μm deep and 400 μm wide PDMS microchannel was used to define the flow path of the Nafion solution (20 wt% solution in lower aliphatic alcohol/H2 O mix, Sigma Aldrich, St. Louis, MO). .. The PDMS chip with microchannels was irreversibly bonded on top of the glass slide by standard plasma bonding.

    In Vivo:

    Article Title: A novel small-molecule compound targeting CD147 inhibits the motility and invasion of hepatocellular carcinoma cells
    Article Snippet: .. For in vivo experiments, AC-73 was dissolved in Cremophor EL/ethanol (50:50; Sigma Cremophor EL, 95% ethyl alcohol) at 4-fold of the highest dose and stored at room temperature. .. Cell culture The HCC cell lines SMMC-7721 (7721) and Huh-7 were purchased from the Institute of Cell Biology of Academia Sinica (Shanghai, China) and the Cell Bank of the JCRB (Tokyo, Japan), respectively.

    Molecular Weight:

    Article Title: Investigation of Conical Spinneret in Generating More Dense and Compact Electrospun Nanofibers
    Article Snippet: .. Synthesis, Process Parameters, and Characterization of Nanofibers Poly(vinyl alcohol) (PVA) of molecular weight (M w = 61,000 g/mol) was purchased from Sigma-Aldrich (St. Louis, MO, USA). .. A concentration of 10 wt % PVA solution was prepared by mixing 10 g PVA pellets with 90 mL of distilled water.

    Purification:

    Article Title: Porous silicon-based nanostructured microparticles as degradable supports for solid-phase synthesis and release of oligonucleotides
    Article Snippet: .. Deuterated chloroform (Cambridge Isotope Laboratories, Inc., Andover, MA, USA) was used without further purification. ω-Undecylenyl alcohol (Aldrich) and 10-undecylenic acid were used as received. .. Phosphate buffered saline (PBS) was prepared from sodium chloride (NaCl, 8 g L−1 ; Chemsupply, Port Adelaide, Australia), potassium chloride (KCl, 0.2 g L−1 ; Biolab Scientific, Scoresby, Australia), di-sodium phosphate di-hydrate (Na2 .HPO4 .

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    https://www.bioz.com/result/benzyl benzoate/product/Millipore
    Average 99 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
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