s aureus strains usa300 je2  (ATCC)

Journal: bioRxiv

Article Title: A chimeric, half-life extended lysin with a unique mode of action

doi: 10.64898/2026.05.13.724763

Figure Lengend Snippet: (A) Efficacy of Fc-CHAP-LSN constructs against in vitro formed biofilms. Bacterial quantification of 24 h-cultivated biofilms of S. aureus ATCC 43300 after 24 h exposure to SOC antibiotics or lysins. Concentrations indicated in µg/mL. Statistical analysis was performed using Graphpad’s one-way ANOVA with Dunnett’s correction for multiple comparisons; ****: P<0.0001; ***: P=0.001; **: P<0.01 ns: not significant (B) Graphical representation of the tested lysins in this assay. Cli: clindamycin; Dapto: daptomycin; LOQ: limit of quantification at 500 CFU/mL; Rif: rifampicin; Untr.: untreated control; Van: vancomycin;

Article Snippet: The following strains were used for this study: Staphylococcus aureus ATCC 43300 (ATCC), BAA-1717 (ATCC), NCTC 8178 (NCTC), Staphylococcus epidermidis DSM3269 (DSMZ), S. epidermidis RP62A (ATCC), Staphylococcus lugdunensis DSM4804 (DSMZ), Staphylococcus haemolyticus DSM20263 (DSMZ) and Staphylococcus haemolyticus DSM20265 (DSMZ).

Techniques: Construct, In Vitro, Control

Journal: bioRxiv

Article Title: A chimeric, half-life extended lysin with a unique mode of action

doi: 10.64898/2026.05.13.724763

Figure Lengend Snippet: Efficacy of Fc-CHAP-LSN against in vivo formed biofilms and in sepsis model. (A) Catheters with in vivo formed biofilms of S. aureus ATCC 43300 were explanted from CD1 mice 3 dpi and treated with either 50 µg/mL or 100 µg/mL Fc-L1-CHAP opt -LSN HEKa for 24 h at 37 °C. Rifampicin (10 µg/mL) was used as comparator. (B) Datapoints represent the biofilm bacterial burden in each catheter (n=6). Horizontal bars indicate the median burden for each group. When burden is below LOQ, half a value of LOQ is assigned for graphical and statistical purposes. Brown Forsythe ANOVA with Welch’s correction (GraphPad Prism) was used to calculate statistical significance against vehicle. dpi: days post infection; LOQ: limit of quantification at 10 CFU/catheter. (C) Lysin efficacy in mouse IV sepsis: Immunocompetent male CD1 mice were challenged with 3.23 × 10 7 CFU/mouse of S. aureus NCTC 8178, IV. Lysins were administered IV, twice (1 hpi and 25 hpi) at dose of either 100 µg/mouse or 500 µg/mouse. Vancomycin (Van) was dosed at 25 mg/kg, IV, q12 h, starting from 1 hpi. (D) Points on the graph represent CFU counts in individual mice (n=6), with horizontal lines indicating median CFU burden and interquartile range. Open circles indicate animals that needed to be terminated before planned endpoint due to clinical symptoms. Brown-Forsythe ANOVA with Welch’s correction was used to calculate the significance compared to vehicle group on log transformed data, with P values indicated in the graph. hpi: hours post infection; CFU: colony forming units; LOQ: limit of quantification at 54 CFU/g; For graphical and statistical purposes, values under LOQ were assigned half of the LOQ value.

Article Snippet: The following strains were used for this study: Staphylococcus aureus ATCC 43300 (ATCC), BAA-1717 (ATCC), NCTC 8178 (NCTC), Staphylococcus epidermidis DSM3269 (DSMZ), S. epidermidis RP62A (ATCC), Staphylococcus lugdunensis DSM4804 (DSMZ), Staphylococcus haemolyticus DSM20263 (DSMZ) and Staphylococcus haemolyticus DSM20265 (DSMZ).

Techniques: In Vivo, Infection, Transformation Assay

Journal: bioRxiv

Article Title: A chimeric, half-life extended lysin with a unique mode of action

doi: 10.64898/2026.05.13.724763

Figure Lengend Snippet: LSN-induced LSN-release from Fc-CHAP-LSN. (a) Purified LSN HEKa was incubated at increasing concentrations in 80% human serum and in presence of 10 8 CFU/mL S. aureus ATCC 43300 with (circles) or without (boxes) a fixed concentration of Fc-L1-CHAP opt -LSN HEK (1 µM) for 3h at 37°C. Samples were analyzed by SDS-PAGE followed by Western Blotting to quantify the amounts of free LSN in each reaction (x-axis). The remainder of the reaction was subjected to quantitative plating to determine the log10 CFU reduction relative to a growth control lacking lysins (y-axis). (B) Concentrations of spiked-in purified LSN are plotted against free LSN detected by Western blotting in the experiment described in panel (A).

Article Snippet: The following strains were used for this study: Staphylococcus aureus ATCC 43300 (ATCC), BAA-1717 (ATCC), NCTC 8178 (NCTC), Staphylococcus epidermidis DSM3269 (DSMZ), S. epidermidis RP62A (ATCC), Staphylococcus lugdunensis DSM4804 (DSMZ), Staphylococcus haemolyticus DSM20263 (DSMZ) and Staphylococcus haemolyticus DSM20265 (DSMZ).

Techniques: Purification, Incubation, Concentration Assay, SDS Page, Western Blot, Control