biotinylated goat anti human igg  (Vector Laboratories)


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    Vector Laboratories biotinylated goat anti human igg
    Biotinylated Goat Anti Human Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated goat anti human igg  (Vector Laboratories)


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    Vector Laboratories biotinylated goat anti human igg
    Biotinylated Goat Anti Human Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated igg  (Vector Laboratories)


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    Vector Laboratories biotinylated igg
    The detail information of primers, antibody and siRNA.
    Biotinylated Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated igg/product/Vector Laboratories
    Average 94 stars, based on 1 article reviews
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    1) Product Images from "Melatonin Suppresses Neuropathic Pain via MT2-Dependent and -Independent Pathways in Dorsal Root Ganglia Neurons of Mice"

    Article Title: Melatonin Suppresses Neuropathic Pain via MT2-Dependent and -Independent Pathways in Dorsal Root Ganglia Neurons of Mice

    Journal: Theranostics

    doi: 10.7150/thno.19500

    The detail information of primers, antibody and siRNA.
    Figure Legend Snippet: The detail information of primers, antibody and siRNA.

    Techniques Used: Western Blot, Immunohistochemical staining, Staining, Plasmid Preparation

    biotinylated anti goat igg  (Vector Laboratories)


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  • 94

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    Vector Laboratories biotinylated anti goat igg
    Biotinylated Anti Goat Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated anti goat igg/product/Vector Laboratories
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    anti human igg  (Vector Laboratories)


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    Vector Laboratories anti human igg
    <t>Anti-Hsp72</t> <t>IgG</t> and IgGAM concentrations are elevated in the BALf of IPF patient nonprogressors compared to progressors. Anti-Hsp72 IgG was measured in the serum and BALf using the in-house optimised anti-Hsp72 IgG ELISA and commercial anti-Hsp72 IgGAM ELISA (IPF n = 82, other ILDs n = 46, healthy controls n = 17). BALf concentrations of anti-Hsp72 IgG and IgGAM are standardised to BALf total IgG. Between ILD subgroups and healthy controls, no significant difference was seen in the serum anti-Hsp72 IgG ((a) p = 0.72) or anti-Hsp72 IgGAM ((b) p = 0.24). No significant difference was seen between IPF progressor and nonprogressor patient serum anti-Hsp72 IgG ((a) p = 0.83) or serum anti-Hsp72 IgGAM ((b) p = 0.95) concentrations. Elevated concentrations of BALf anti-Hsp72 IgG ((c) p = 0.014) and anti-Hsp72 IgGAM ((d) p = 0.005) were seen in IPF nonprogressors compared to progressors. Statistical analysis was performed using Kruskal-Wallis with Dunn's posttest and Mann-Whitney U tests.
    Anti Human Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti human igg/product/Vector Laboratories
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    anti human igg - by Bioz Stars, 2024-05
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    1) Product Images from "Intrapulmonary Autoantibodies to HSP72 Are Associated with Improved Outcomes in IPF"

    Article Title: Intrapulmonary Autoantibodies to HSP72 Are Associated with Improved Outcomes in IPF

    Journal: Journal of Immunology Research

    doi: 10.1155/2019/1845128

    Anti-Hsp72 IgG and IgGAM concentrations are elevated in the BALf of IPF patient nonprogressors compared to progressors. Anti-Hsp72 IgG was measured in the serum and BALf using the in-house optimised anti-Hsp72 IgG ELISA and commercial anti-Hsp72 IgGAM ELISA (IPF n = 82, other ILDs n = 46, healthy controls n = 17). BALf concentrations of anti-Hsp72 IgG and IgGAM are standardised to BALf total IgG. Between ILD subgroups and healthy controls, no significant difference was seen in the serum anti-Hsp72 IgG ((a) p = 0.72) or anti-Hsp72 IgGAM ((b) p = 0.24). No significant difference was seen between IPF progressor and nonprogressor patient serum anti-Hsp72 IgG ((a) p = 0.83) or serum anti-Hsp72 IgGAM ((b) p = 0.95) concentrations. Elevated concentrations of BALf anti-Hsp72 IgG ((c) p = 0.014) and anti-Hsp72 IgGAM ((d) p = 0.005) were seen in IPF nonprogressors compared to progressors. Statistical analysis was performed using Kruskal-Wallis with Dunn's posttest and Mann-Whitney U tests.
    Figure Legend Snippet: Anti-Hsp72 IgG and IgGAM concentrations are elevated in the BALf of IPF patient nonprogressors compared to progressors. Anti-Hsp72 IgG was measured in the serum and BALf using the in-house optimised anti-Hsp72 IgG ELISA and commercial anti-Hsp72 IgGAM ELISA (IPF n = 82, other ILDs n = 46, healthy controls n = 17). BALf concentrations of anti-Hsp72 IgG and IgGAM are standardised to BALf total IgG. Between ILD subgroups and healthy controls, no significant difference was seen in the serum anti-Hsp72 IgG ((a) p = 0.72) or anti-Hsp72 IgGAM ((b) p = 0.24). No significant difference was seen between IPF progressor and nonprogressor patient serum anti-Hsp72 IgG ((a) p = 0.83) or serum anti-Hsp72 IgGAM ((b) p = 0.95) concentrations. Elevated concentrations of BALf anti-Hsp72 IgG ((c) p = 0.014) and anti-Hsp72 IgGAM ((d) p = 0.005) were seen in IPF nonprogressors compared to progressors. Statistical analysis was performed using Kruskal-Wallis with Dunn's posttest and Mann-Whitney U tests.

    Techniques Used: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    IPF patients with raised concentrations of BALf anti-Hsp72 IgGAM have longer survival rates. Total BALf IgG, IgA and anti-Hsp72 IgG IgGAM were split between high (red lines) and low (black lines) expression rates by the median value. A Kaplan-Meier survival curve from the date of the BAL sample was made to either death or the census date. No association with survival was seen in total IgG ((a) HR 0.82, 95% CI 0.45-1.49, p = 0.51) or anti-Hsp72 IgG ((c) HR 1.38, 95% CI 0.50-1.74, p = 0.33). Total IgA ((b) HR 2.09, 95% CI 1.01-4.32, p = 0.048) and anti-Hsp72 IgGAM ((d) HR 0.44, 95% CI 0.20-0.92, p = 0.018) associated with improved patient survival. Total IgA and anti-Hsp72 IgGAM were reanalysed using a Cox proportional hazard model adjusting for age, sex, smoking, and percentage predicted VC and T L CO. After adjustment, total IgA showed no association with patient survival (adjusted HR 0.85, p = 0.77), whilst elevated anti-Hsp72 IgGAM did show a significant association with survival (adjusted HR 0.62, 95% CI 0.45-0.85, p = 0.003).
    Figure Legend Snippet: IPF patients with raised concentrations of BALf anti-Hsp72 IgGAM have longer survival rates. Total BALf IgG, IgA and anti-Hsp72 IgG IgGAM were split between high (red lines) and low (black lines) expression rates by the median value. A Kaplan-Meier survival curve from the date of the BAL sample was made to either death or the census date. No association with survival was seen in total IgG ((a) HR 0.82, 95% CI 0.45-1.49, p = 0.51) or anti-Hsp72 IgG ((c) HR 1.38, 95% CI 0.50-1.74, p = 0.33). Total IgA ((b) HR 2.09, 95% CI 1.01-4.32, p = 0.048) and anti-Hsp72 IgGAM ((d) HR 0.44, 95% CI 0.20-0.92, p = 0.018) associated with improved patient survival. Total IgA and anti-Hsp72 IgGAM were reanalysed using a Cox proportional hazard model adjusting for age, sex, smoking, and percentage predicted VC and T L CO. After adjustment, total IgA showed no association with patient survival (adjusted HR 0.85, p = 0.77), whilst elevated anti-Hsp72 IgGAM did show a significant association with survival (adjusted HR 0.62, 95% CI 0.45-0.85, p = 0.003).

    Techniques Used: Expressing

    Hsp72-IgG complexes induce CXCL8 secretion from in vitro MDM culture. MDM cell culture supernatant was analysed for CXCL8 (a) and CCL18 (b) by ELISA after treatment with 1 μ g/ml IgG isolated from BALf of IPF patients ± 100 ng/ml Hsp72 (IPF patient n = 6, repeated twice) for 24 hours. 1 μ g/ml of BALf IgG did not lead to any overt fold change in CXCL8 secretion in comparison to untreated cells, but preincubation of IgG with Hsp72 led to increased CXCL8 secretion ((a), p = 0.031). A 4-fold increase in MDM CCL18 secretion was seen in response to BALf IgG, with Hsp72 preincubation having no effect ((b), p = 0.84). Statistical tests to compare BALf IgG with preincubated BALf IgG used a Wilcoxon test; no statistical comparison was made with untreated cells.
    Figure Legend Snippet: Hsp72-IgG complexes induce CXCL8 secretion from in vitro MDM culture. MDM cell culture supernatant was analysed for CXCL8 (a) and CCL18 (b) by ELISA after treatment with 1 μ g/ml IgG isolated from BALf of IPF patients ± 100 ng/ml Hsp72 (IPF patient n = 6, repeated twice) for 24 hours. 1 μ g/ml of BALf IgG did not lead to any overt fold change in CXCL8 secretion in comparison to untreated cells, but preincubation of IgG with Hsp72 led to increased CXCL8 secretion ((a), p = 0.031). A 4-fold increase in MDM CCL18 secretion was seen in response to BALf IgG, with Hsp72 preincubation having no effect ((b), p = 0.84). Statistical tests to compare BALf IgG with preincubated BALf IgG used a Wilcoxon test; no statistical comparison was made with untreated cells.

    Techniques Used: In Vitro, Cell Culture, Enzyme-linked Immunosorbent Assay, Isolation

    biotinylated goat anti human igg  (Vector Laboratories)


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    Vector Laboratories biotinylated goat anti human igg
    Biotinylated Goat Anti Human Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated anti goat igg  (Vector Laboratories)


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    Vector Laboratories biotinylated anti goat igg
    Biotinylated Anti Goat Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated goat anti human igg  (Vector Laboratories)


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    Vector Laboratories biotinylated goat anti human igg
    Biotinylated Goat Anti Human Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    goat anti human igg h l biotinylated antibody  (Vector Laboratories)


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    Vector Laboratories goat anti human igg h l biotinylated antibody
    Goat Anti Human Igg H L Biotinylated Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated igg  (Vector Laboratories)


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    Vector Laboratories biotinylated igg
    Biotinylated Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    igg  (Vector Laboratories)


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    Vector Laboratories biotinylated goat anti human igg
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    Vector Laboratories biotinylated igg
    The detail information of primers, antibody and siRNA.
    Biotinylated Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories anti human igg
    <t>Anti-Hsp72</t> <t>IgG</t> and IgGAM concentrations are elevated in the BALf of IPF patient nonprogressors compared to progressors. Anti-Hsp72 IgG was measured in the serum and BALf using the in-house optimised anti-Hsp72 IgG ELISA and commercial anti-Hsp72 IgGAM ELISA (IPF n = 82, other ILDs n = 46, healthy controls n = 17). BALf concentrations of anti-Hsp72 IgG and IgGAM are standardised to BALf total IgG. Between ILD subgroups and healthy controls, no significant difference was seen in the serum anti-Hsp72 IgG ((a) p = 0.72) or anti-Hsp72 IgGAM ((b) p = 0.24). No significant difference was seen between IPF progressor and nonprogressor patient serum anti-Hsp72 IgG ((a) p = 0.83) or serum anti-Hsp72 IgGAM ((b) p = 0.95) concentrations. Elevated concentrations of BALf anti-Hsp72 IgG ((c) p = 0.014) and anti-Hsp72 IgGAM ((d) p = 0.005) were seen in IPF nonprogressors compared to progressors. Statistical analysis was performed using Kruskal-Wallis with Dunn's posttest and Mann-Whitney U tests.
    Anti Human Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories goat anti human igg h l biotinylated antibody
    <t>Anti-Hsp72</t> <t>IgG</t> and IgGAM concentrations are elevated in the BALf of IPF patient nonprogressors compared to progressors. Anti-Hsp72 IgG was measured in the serum and BALf using the in-house optimised anti-Hsp72 IgG ELISA and commercial anti-Hsp72 IgGAM ELISA (IPF n = 82, other ILDs n = 46, healthy controls n = 17). BALf concentrations of anti-Hsp72 IgG and IgGAM are standardised to BALf total IgG. Between ILD subgroups and healthy controls, no significant difference was seen in the serum anti-Hsp72 IgG ((a) p = 0.72) or anti-Hsp72 IgGAM ((b) p = 0.24). No significant difference was seen between IPF progressor and nonprogressor patient serum anti-Hsp72 IgG ((a) p = 0.83) or serum anti-Hsp72 IgGAM ((b) p = 0.95) concentrations. Elevated concentrations of BALf anti-Hsp72 IgG ((c) p = 0.014) and anti-Hsp72 IgGAM ((d) p = 0.005) were seen in IPF nonprogressors compared to progressors. Statistical analysis was performed using Kruskal-Wallis with Dunn's posttest and Mann-Whitney U tests.
    Goat Anti Human Igg H L Biotinylated Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories igg
    <t>Anti-Hsp72</t> <t>IgG</t> and IgGAM concentrations are elevated in the BALf of IPF patient nonprogressors compared to progressors. Anti-Hsp72 IgG was measured in the serum and BALf using the in-house optimised anti-Hsp72 IgG ELISA and commercial anti-Hsp72 IgGAM ELISA (IPF n = 82, other ILDs n = 46, healthy controls n = 17). BALf concentrations of anti-Hsp72 IgG and IgGAM are standardised to BALf total IgG. Between ILD subgroups and healthy controls, no significant difference was seen in the serum anti-Hsp72 IgG ((a) p = 0.72) or anti-Hsp72 IgGAM ((b) p = 0.24). No significant difference was seen between IPF progressor and nonprogressor patient serum anti-Hsp72 IgG ((a) p = 0.83) or serum anti-Hsp72 IgGAM ((b) p = 0.95) concentrations. Elevated concentrations of BALf anti-Hsp72 IgG ((c) p = 0.014) and anti-Hsp72 IgGAM ((d) p = 0.005) were seen in IPF nonprogressors compared to progressors. Statistical analysis was performed using Kruskal-Wallis with Dunn's posttest and Mann-Whitney U tests.
    Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    The detail information of primers, antibody and siRNA.

    Journal: Theranostics

    Article Title: Melatonin Suppresses Neuropathic Pain via MT2-Dependent and -Independent Pathways in Dorsal Root Ganglia Neurons of Mice

    doi: 10.7150/thno.19500

    Figure Lengend Snippet: The detail information of primers, antibody and siRNA.

    Article Snippet: Subsequently, sections were incubated with biotinylated IgG diluted in PBS for 4 h followed by analysis with the avidin-biotin-peroxidase complex (ABC) Elite Kit (1:100; Vector, Burlingame, CA, USA) in 0.01 M PBS (pH=7.4) for 2 h. Finally, antibody immunoreactivity was visualized in 0.05M Tris-HCl buffer (pH=7.6) containing 0.04% DAB and 0.003% H 2 O 2 followed by hematoxylin staining of nuclei.

    Techniques: Western Blot, Immunohistochemical staining, Staining, Plasmid Preparation

    Anti-Hsp72 IgG and IgGAM concentrations are elevated in the BALf of IPF patient nonprogressors compared to progressors. Anti-Hsp72 IgG was measured in the serum and BALf using the in-house optimised anti-Hsp72 IgG ELISA and commercial anti-Hsp72 IgGAM ELISA (IPF n = 82, other ILDs n = 46, healthy controls n = 17). BALf concentrations of anti-Hsp72 IgG and IgGAM are standardised to BALf total IgG. Between ILD subgroups and healthy controls, no significant difference was seen in the serum anti-Hsp72 IgG ((a) p = 0.72) or anti-Hsp72 IgGAM ((b) p = 0.24). No significant difference was seen between IPF progressor and nonprogressor patient serum anti-Hsp72 IgG ((a) p = 0.83) or serum anti-Hsp72 IgGAM ((b) p = 0.95) concentrations. Elevated concentrations of BALf anti-Hsp72 IgG ((c) p = 0.014) and anti-Hsp72 IgGAM ((d) p = 0.005) were seen in IPF nonprogressors compared to progressors. Statistical analysis was performed using Kruskal-Wallis with Dunn's posttest and Mann-Whitney U tests.

    Journal: Journal of Immunology Research

    Article Title: Intrapulmonary Autoantibodies to HSP72 Are Associated with Improved Outcomes in IPF

    doi: 10.1155/2019/1845128

    Figure Lengend Snippet: Anti-Hsp72 IgG and IgGAM concentrations are elevated in the BALf of IPF patient nonprogressors compared to progressors. Anti-Hsp72 IgG was measured in the serum and BALf using the in-house optimised anti-Hsp72 IgG ELISA and commercial anti-Hsp72 IgGAM ELISA (IPF n = 82, other ILDs n = 46, healthy controls n = 17). BALf concentrations of anti-Hsp72 IgG and IgGAM are standardised to BALf total IgG. Between ILD subgroups and healthy controls, no significant difference was seen in the serum anti-Hsp72 IgG ((a) p = 0.72) or anti-Hsp72 IgGAM ((b) p = 0.24). No significant difference was seen between IPF progressor and nonprogressor patient serum anti-Hsp72 IgG ((a) p = 0.83) or serum anti-Hsp72 IgGAM ((b) p = 0.95) concentrations. Elevated concentrations of BALf anti-Hsp72 IgG ((c) p = 0.014) and anti-Hsp72 IgGAM ((d) p = 0.005) were seen in IPF nonprogressors compared to progressors. Statistical analysis was performed using Kruskal-Wallis with Dunn's posttest and Mann-Whitney U tests.

    Article Snippet: Detection of anti-Hsp72 IgG was performed using a biotin-conjugated anti-human IgG (Vector Laboratories, BA-3000), incubated for 2 hours.

    Techniques: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    IPF patients with raised concentrations of BALf anti-Hsp72 IgGAM have longer survival rates. Total BALf IgG, IgA and anti-Hsp72 IgG IgGAM were split between high (red lines) and low (black lines) expression rates by the median value. A Kaplan-Meier survival curve from the date of the BAL sample was made to either death or the census date. No association with survival was seen in total IgG ((a) HR 0.82, 95% CI 0.45-1.49, p = 0.51) or anti-Hsp72 IgG ((c) HR 1.38, 95% CI 0.50-1.74, p = 0.33). Total IgA ((b) HR 2.09, 95% CI 1.01-4.32, p = 0.048) and anti-Hsp72 IgGAM ((d) HR 0.44, 95% CI 0.20-0.92, p = 0.018) associated with improved patient survival. Total IgA and anti-Hsp72 IgGAM were reanalysed using a Cox proportional hazard model adjusting for age, sex, smoking, and percentage predicted VC and T L CO. After adjustment, total IgA showed no association with patient survival (adjusted HR 0.85, p = 0.77), whilst elevated anti-Hsp72 IgGAM did show a significant association with survival (adjusted HR 0.62, 95% CI 0.45-0.85, p = 0.003).

    Journal: Journal of Immunology Research

    Article Title: Intrapulmonary Autoantibodies to HSP72 Are Associated with Improved Outcomes in IPF

    doi: 10.1155/2019/1845128

    Figure Lengend Snippet: IPF patients with raised concentrations of BALf anti-Hsp72 IgGAM have longer survival rates. Total BALf IgG, IgA and anti-Hsp72 IgG IgGAM were split between high (red lines) and low (black lines) expression rates by the median value. A Kaplan-Meier survival curve from the date of the BAL sample was made to either death or the census date. No association with survival was seen in total IgG ((a) HR 0.82, 95% CI 0.45-1.49, p = 0.51) or anti-Hsp72 IgG ((c) HR 1.38, 95% CI 0.50-1.74, p = 0.33). Total IgA ((b) HR 2.09, 95% CI 1.01-4.32, p = 0.048) and anti-Hsp72 IgGAM ((d) HR 0.44, 95% CI 0.20-0.92, p = 0.018) associated with improved patient survival. Total IgA and anti-Hsp72 IgGAM were reanalysed using a Cox proportional hazard model adjusting for age, sex, smoking, and percentage predicted VC and T L CO. After adjustment, total IgA showed no association with patient survival (adjusted HR 0.85, p = 0.77), whilst elevated anti-Hsp72 IgGAM did show a significant association with survival (adjusted HR 0.62, 95% CI 0.45-0.85, p = 0.003).

    Article Snippet: Detection of anti-Hsp72 IgG was performed using a biotin-conjugated anti-human IgG (Vector Laboratories, BA-3000), incubated for 2 hours.

    Techniques: Expressing

    Hsp72-IgG complexes induce CXCL8 secretion from in vitro MDM culture. MDM cell culture supernatant was analysed for CXCL8 (a) and CCL18 (b) by ELISA after treatment with 1 μ g/ml IgG isolated from BALf of IPF patients ± 100 ng/ml Hsp72 (IPF patient n = 6, repeated twice) for 24 hours. 1 μ g/ml of BALf IgG did not lead to any overt fold change in CXCL8 secretion in comparison to untreated cells, but preincubation of IgG with Hsp72 led to increased CXCL8 secretion ((a), p = 0.031). A 4-fold increase in MDM CCL18 secretion was seen in response to BALf IgG, with Hsp72 preincubation having no effect ((b), p = 0.84). Statistical tests to compare BALf IgG with preincubated BALf IgG used a Wilcoxon test; no statistical comparison was made with untreated cells.

    Journal: Journal of Immunology Research

    Article Title: Intrapulmonary Autoantibodies to HSP72 Are Associated with Improved Outcomes in IPF

    doi: 10.1155/2019/1845128

    Figure Lengend Snippet: Hsp72-IgG complexes induce CXCL8 secretion from in vitro MDM culture. MDM cell culture supernatant was analysed for CXCL8 (a) and CCL18 (b) by ELISA after treatment with 1 μ g/ml IgG isolated from BALf of IPF patients ± 100 ng/ml Hsp72 (IPF patient n = 6, repeated twice) for 24 hours. 1 μ g/ml of BALf IgG did not lead to any overt fold change in CXCL8 secretion in comparison to untreated cells, but preincubation of IgG with Hsp72 led to increased CXCL8 secretion ((a), p = 0.031). A 4-fold increase in MDM CCL18 secretion was seen in response to BALf IgG, with Hsp72 preincubation having no effect ((b), p = 0.84). Statistical tests to compare BALf IgG with preincubated BALf IgG used a Wilcoxon test; no statistical comparison was made with untreated cells.

    Article Snippet: Detection of anti-Hsp72 IgG was performed using a biotin-conjugated anti-human IgG (Vector Laboratories, BA-3000), incubated for 2 hours.

    Techniques: In Vitro, Cell Culture, Enzyme-linked Immunosorbent Assay, Isolation