b16f10 cd70  (ATCC)


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    Structured Review

    ATCC b16f10 cd70
    <t>CD70</t> ectopic expression in human melanomas. Representative images of CD70 expression after staining by IHC paraffin-embedded melanoma human biopsies ( A ) or staining of cryostat sections of frozen melanoma tumour specimens ( B ). Negative staining corresponded to CD70 expression in <2% of melanoma cells, moderate staining in 2–20% and strong staining in more than 20%. ( C ) Three slides of the same melanoma fragment showing that CD70 expression is in the tumour cells. The slides were labelled with anti-CD70 mAb, anti-MelanA or anti-KBA.62 antitumour antibodies. ( D ) Primary human melanomas contain more CD70+ tumour cells than metastases as quantified in paraffin-embedded melanoma biopsies. ( E ) The intensity of CD70 expression is also stronger in primary melanomas than in metastases. P -values were evaluated using the Mann–Whitney test ( D ) or the χ 2 -test ( E ). ** P <0.005; *** P <0.001.
    B16f10 Cd70, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/b16f10 cd70/product/ATCC
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    b16f10 cd70 - by Bioz Stars, 2024-04
    93/100 stars

    Images

    1) Product Images from "Melanoma-expressed CD70 is involved in invasion and metastasis"

    Article Title: Melanoma-expressed CD70 is involved in invasion and metastasis

    Journal: British Journal of Cancer

    doi: 10.1038/bjc.2015.412

    CD70 ectopic expression in human melanomas. Representative images of CD70 expression after staining by IHC paraffin-embedded melanoma human biopsies ( A ) or staining of cryostat sections of frozen melanoma tumour specimens ( B ). Negative staining corresponded to CD70 expression in <2% of melanoma cells, moderate staining in 2–20% and strong staining in more than 20%. ( C ) Three slides of the same melanoma fragment showing that CD70 expression is in the tumour cells. The slides were labelled with anti-CD70 mAb, anti-MelanA or anti-KBA.62 antitumour antibodies. ( D ) Primary human melanomas contain more CD70+ tumour cells than metastases as quantified in paraffin-embedded melanoma biopsies. ( E ) The intensity of CD70 expression is also stronger in primary melanomas than in metastases. P -values were evaluated using the Mann–Whitney test ( D ) or the χ 2 -test ( E ). ** P <0.005; *** P <0.001.
    Figure Legend Snippet: CD70 ectopic expression in human melanomas. Representative images of CD70 expression after staining by IHC paraffin-embedded melanoma human biopsies ( A ) or staining of cryostat sections of frozen melanoma tumour specimens ( B ). Negative staining corresponded to CD70 expression in <2% of melanoma cells, moderate staining in 2–20% and strong staining in more than 20%. ( C ) Three slides of the same melanoma fragment showing that CD70 expression is in the tumour cells. The slides were labelled with anti-CD70 mAb, anti-MelanA or anti-KBA.62 antitumour antibodies. ( D ) Primary human melanomas contain more CD70+ tumour cells than metastases as quantified in paraffin-embedded melanoma biopsies. ( E ) The intensity of CD70 expression is also stronger in primary melanomas than in metastases. P -values were evaluated using the Mann–Whitney test ( D ) or the χ 2 -test ( E ). ** P <0.005; *** P <0.001.

    Techniques Used: Expressing, Staining, Negative Staining, MANN-WHITNEY

    CD70 expression decreases with time and disease progression in human melanomas. ( A ) CD70 membrane expression measured by flow cytometry in three representative melanoma cell lines: LB1319-MEL; LB39-MEL; and LB39-MEL clones CD70+ vs CD70−. ( B ) Decrease of membrane CD70 expression in three melanoma cell lines derived from the same patient: LB33-MEL.A (skin, 1988); LB33-MEL.B (lymph node, 1993); and LB33-MEL.D (intestine, 1999). ( C ) Decrease with disease progression of melanoma-expressed CD70: primary tumours, skin and lymph node metastases from the same patient C121084. ( D ) Monomeric (CD70 M) and trimeric (CD70 T) forms of CD70 detected by western blot in LB1319-MEL (total, cytosolic and membrane fractions), B16F10-wt and B16F10-CD70 cells.
    Figure Legend Snippet: CD70 expression decreases with time and disease progression in human melanomas. ( A ) CD70 membrane expression measured by flow cytometry in three representative melanoma cell lines: LB1319-MEL; LB39-MEL; and LB39-MEL clones CD70+ vs CD70−. ( B ) Decrease of membrane CD70 expression in three melanoma cell lines derived from the same patient: LB33-MEL.A (skin, 1988); LB33-MEL.B (lymph node, 1993); and LB33-MEL.D (intestine, 1999). ( C ) Decrease with disease progression of melanoma-expressed CD70: primary tumours, skin and lymph node metastases from the same patient C121084. ( D ) Monomeric (CD70 M) and trimeric (CD70 T) forms of CD70 detected by western blot in LB1319-MEL (total, cytosolic and membrane fractions), B16F10-wt and B16F10-CD70 cells.

    Techniques Used: Expressing, Flow Cytometry, Clone Assay, Derivative Assay, Western Blot

    CD70 expression is associated with decreased in vivo metastatic capacity. ( A ) C57BL/6, C57BL/6 IFN- γ KO and NMRI nu/nu mice were injected i.v. with B16F10-wt (blue) or B16F10-CD70 (red) cells and pulmonary metastases were quantified, showing that B16F10-CD70 cells induced less metastases implantations. ( B ) Representative lung photomicrographs (left) and enlargement of metastatic areas (right) of C57BL/6 mice injected i.v. with B16F10-wt (top) or B16F10-CD70 (bottom) cells. Arrows indicate metastases. P -values were evaluated using the t -test. *** P <0.001.
    Figure Legend Snippet: CD70 expression is associated with decreased in vivo metastatic capacity. ( A ) C57BL/6, C57BL/6 IFN- γ KO and NMRI nu/nu mice were injected i.v. with B16F10-wt (blue) or B16F10-CD70 (red) cells and pulmonary metastases were quantified, showing that B16F10-CD70 cells induced less metastases implantations. ( B ) Representative lung photomicrographs (left) and enlargement of metastatic areas (right) of C57BL/6 mice injected i.v. with B16F10-wt (top) or B16F10-CD70 (bottom) cells. Arrows indicate metastases. P -values were evaluated using the t -test. *** P <0.001.

    Techniques Used: Expressing, In Vivo, Injection

    In melanoma cells CD70 expression is associated with decreased in vitro migration and invasion capacities. Decreased in vitro migration ( A ) and invasion ( B ) capacities of B16F10-CD70+ cells analysed using, respectively, transwells and invasion chambers with B16F10-wt vs B16F10-CD70 cells. In the upper chamber the medium was or not (NT) supplemented with Ctrl Ig (Ct) or anti-CD70 mAb (QA). Decreased in vitro migration capacity of B16F10-CD70+ cells was analysed by wound-healing experiments done with B16F10-wt and B16F10-CD70 cells ( C ). Decreased in vitro migration ( D ) and invasion ( E ) capacities of CD70+ human cells: LB39-MEL CD70− clone (blue), LB39-MEL CD70+ clone (red) and LB1319-MEL cells (black). Results ( A , B , D and E ) are expressed as mean values ( n =7). P -values were evaluated using the Tukey ANOVA test. * P <0.05; ** P <0.005; *** P <0.001.
    Figure Legend Snippet: In melanoma cells CD70 expression is associated with decreased in vitro migration and invasion capacities. Decreased in vitro migration ( A ) and invasion ( B ) capacities of B16F10-CD70+ cells analysed using, respectively, transwells and invasion chambers with B16F10-wt vs B16F10-CD70 cells. In the upper chamber the medium was or not (NT) supplemented with Ctrl Ig (Ct) or anti-CD70 mAb (QA). Decreased in vitro migration capacity of B16F10-CD70+ cells was analysed by wound-healing experiments done with B16F10-wt and B16F10-CD70 cells ( C ). Decreased in vitro migration ( D ) and invasion ( E ) capacities of CD70+ human cells: LB39-MEL CD70− clone (blue), LB39-MEL CD70+ clone (red) and LB1319-MEL cells (black). Results ( A , B , D and E ) are expressed as mean values ( n =7). P -values were evaluated using the Tukey ANOVA test. * P <0.05; ** P <0.005; *** P <0.001.

    Techniques Used: Expressing, In Vitro, Migration

    CD70 signalling modulates MAPK activation, RhoE and ROCK1 expression, and cytoskeleton formation. Western blot analysis ( A ) and quantification ( B ) in arbitrary units (a.u.) of CD70 M decrease and CD70 T increase in LB1319-MEL cells incubated with anti-CD70 mAb (QA). Controls are untreated (NT) and Ctrl Ig (Ct)-incubated LB1319-MEL cells. ( C ) Western blots analyses of the MEK/ERK pathway activation and RhoE overexpression in these LB1319-MEL cells. ( D ) The 3D invasion using LB1319-MEL spheroids treated with the Abs plus MEK inhibitor U0126, showing anti-CD70-induced invasion enhancement and U0126-induced invasion inhibition. ( E ) Western blot quantification of total ROCK1 and ROCK2 in LB1319-MEL cells incubated with Ctrl or anti-CD70 mAb, showing anti-CD70-induced ROCK1 inhibition. ( F ) Disappearance of stress fibres (actin) and focal adhesions (vinculin) in LB1319-MEL cells treated with anti-CD70 mAb for 48 h. Results in B and E are expressed as mean values ( n =3). P -values were evaluated using the Tukey ANOVA test ( B ) or t -test ( E ). ** P <0.005.
    Figure Legend Snippet: CD70 signalling modulates MAPK activation, RhoE and ROCK1 expression, and cytoskeleton formation. Western blot analysis ( A ) and quantification ( B ) in arbitrary units (a.u.) of CD70 M decrease and CD70 T increase in LB1319-MEL cells incubated with anti-CD70 mAb (QA). Controls are untreated (NT) and Ctrl Ig (Ct)-incubated LB1319-MEL cells. ( C ) Western blots analyses of the MEK/ERK pathway activation and RhoE overexpression in these LB1319-MEL cells. ( D ) The 3D invasion using LB1319-MEL spheroids treated with the Abs plus MEK inhibitor U0126, showing anti-CD70-induced invasion enhancement and U0126-induced invasion inhibition. ( E ) Western blot quantification of total ROCK1 and ROCK2 in LB1319-MEL cells incubated with Ctrl or anti-CD70 mAb, showing anti-CD70-induced ROCK1 inhibition. ( F ) Disappearance of stress fibres (actin) and focal adhesions (vinculin) in LB1319-MEL cells treated with anti-CD70 mAb for 48 h. Results in B and E are expressed as mean values ( n =3). P -values were evaluated using the Tukey ANOVA test ( B ) or t -test ( E ). ** P <0.005.

    Techniques Used: Activation Assay, Expressing, Western Blot, Incubation, Over Expression, Inhibition

    b16f10 cd70  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
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    Structured Review

    ATCC b16f10 cd70
    <t>CD70</t> ectopic expression in human melanomas. Representative images of CD70 expression after staining by IHC paraffin-embedded melanoma human biopsies ( A ) or staining of cryostat sections of frozen melanoma tumour specimens ( B ). Negative staining corresponded to CD70 expression in <2% of melanoma cells, moderate staining in 2–20% and strong staining in more than 20%. ( C ) Three slides of the same melanoma fragment showing that CD70 expression is in the tumour cells. The slides were labelled with anti-CD70 mAb, anti-MelanA or anti-KBA.62 antitumour antibodies. ( D ) Primary human melanomas contain more CD70+ tumour cells than metastases as quantified in paraffin-embedded melanoma biopsies. ( E ) The intensity of CD70 expression is also stronger in primary melanomas than in metastases. P -values were evaluated using the Mann–Whitney test ( D ) or the χ 2 -test ( E ). ** P <0.005; *** P <0.001.
    B16f10 Cd70, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/b16f10 cd70/product/ATCC
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    b16f10 cd70 - by Bioz Stars, 2024-04
    93/100 stars

    Images

    1) Product Images from "Melanoma-expressed CD70 is involved in invasion and metastasis"

    Article Title: Melanoma-expressed CD70 is involved in invasion and metastasis

    Journal: British Journal of Cancer

    doi: 10.1038/bjc.2015.412

    CD70 ectopic expression in human melanomas. Representative images of CD70 expression after staining by IHC paraffin-embedded melanoma human biopsies ( A ) or staining of cryostat sections of frozen melanoma tumour specimens ( B ). Negative staining corresponded to CD70 expression in <2% of melanoma cells, moderate staining in 2–20% and strong staining in more than 20%. ( C ) Three slides of the same melanoma fragment showing that CD70 expression is in the tumour cells. The slides were labelled with anti-CD70 mAb, anti-MelanA or anti-KBA.62 antitumour antibodies. ( D ) Primary human melanomas contain more CD70+ tumour cells than metastases as quantified in paraffin-embedded melanoma biopsies. ( E ) The intensity of CD70 expression is also stronger in primary melanomas than in metastases. P -values were evaluated using the Mann–Whitney test ( D ) or the χ 2 -test ( E ). ** P <0.005; *** P <0.001.
    Figure Legend Snippet: CD70 ectopic expression in human melanomas. Representative images of CD70 expression after staining by IHC paraffin-embedded melanoma human biopsies ( A ) or staining of cryostat sections of frozen melanoma tumour specimens ( B ). Negative staining corresponded to CD70 expression in <2% of melanoma cells, moderate staining in 2–20% and strong staining in more than 20%. ( C ) Three slides of the same melanoma fragment showing that CD70 expression is in the tumour cells. The slides were labelled with anti-CD70 mAb, anti-MelanA or anti-KBA.62 antitumour antibodies. ( D ) Primary human melanomas contain more CD70+ tumour cells than metastases as quantified in paraffin-embedded melanoma biopsies. ( E ) The intensity of CD70 expression is also stronger in primary melanomas than in metastases. P -values were evaluated using the Mann–Whitney test ( D ) or the χ 2 -test ( E ). ** P <0.005; *** P <0.001.

    Techniques Used: Expressing, Staining, Negative Staining, MANN-WHITNEY

    CD70 expression decreases with time and disease progression in human melanomas. ( A ) CD70 membrane expression measured by flow cytometry in three representative melanoma cell lines: LB1319-MEL; LB39-MEL; and LB39-MEL clones CD70+ vs CD70−. ( B ) Decrease of membrane CD70 expression in three melanoma cell lines derived from the same patient: LB33-MEL.A (skin, 1988); LB33-MEL.B (lymph node, 1993); and LB33-MEL.D (intestine, 1999). ( C ) Decrease with disease progression of melanoma-expressed CD70: primary tumours, skin and lymph node metastases from the same patient C121084. ( D ) Monomeric (CD70 M) and trimeric (CD70 T) forms of CD70 detected by western blot in LB1319-MEL (total, cytosolic and membrane fractions), B16F10-wt and B16F10-CD70 cells.
    Figure Legend Snippet: CD70 expression decreases with time and disease progression in human melanomas. ( A ) CD70 membrane expression measured by flow cytometry in three representative melanoma cell lines: LB1319-MEL; LB39-MEL; and LB39-MEL clones CD70+ vs CD70−. ( B ) Decrease of membrane CD70 expression in three melanoma cell lines derived from the same patient: LB33-MEL.A (skin, 1988); LB33-MEL.B (lymph node, 1993); and LB33-MEL.D (intestine, 1999). ( C ) Decrease with disease progression of melanoma-expressed CD70: primary tumours, skin and lymph node metastases from the same patient C121084. ( D ) Monomeric (CD70 M) and trimeric (CD70 T) forms of CD70 detected by western blot in LB1319-MEL (total, cytosolic and membrane fractions), B16F10-wt and B16F10-CD70 cells.

    Techniques Used: Expressing, Flow Cytometry, Clone Assay, Derivative Assay, Western Blot

    CD70 expression is associated with decreased in vivo metastatic capacity. ( A ) C57BL/6, C57BL/6 IFN- γ KO and NMRI nu/nu mice were injected i.v. with B16F10-wt (blue) or B16F10-CD70 (red) cells and pulmonary metastases were quantified, showing that B16F10-CD70 cells induced less metastases implantations. ( B ) Representative lung photomicrographs (left) and enlargement of metastatic areas (right) of C57BL/6 mice injected i.v. with B16F10-wt (top) or B16F10-CD70 (bottom) cells. Arrows indicate metastases. P -values were evaluated using the t -test. *** P <0.001.
    Figure Legend Snippet: CD70 expression is associated with decreased in vivo metastatic capacity. ( A ) C57BL/6, C57BL/6 IFN- γ KO and NMRI nu/nu mice were injected i.v. with B16F10-wt (blue) or B16F10-CD70 (red) cells and pulmonary metastases were quantified, showing that B16F10-CD70 cells induced less metastases implantations. ( B ) Representative lung photomicrographs (left) and enlargement of metastatic areas (right) of C57BL/6 mice injected i.v. with B16F10-wt (top) or B16F10-CD70 (bottom) cells. Arrows indicate metastases. P -values were evaluated using the t -test. *** P <0.001.

    Techniques Used: Expressing, In Vivo, Injection

    In melanoma cells CD70 expression is associated with decreased in vitro migration and invasion capacities. Decreased in vitro migration ( A ) and invasion ( B ) capacities of B16F10-CD70+ cells analysed using, respectively, transwells and invasion chambers with B16F10-wt vs B16F10-CD70 cells. In the upper chamber the medium was or not (NT) supplemented with Ctrl Ig (Ct) or anti-CD70 mAb (QA). Decreased in vitro migration capacity of B16F10-CD70+ cells was analysed by wound-healing experiments done with B16F10-wt and B16F10-CD70 cells ( C ). Decreased in vitro migration ( D ) and invasion ( E ) capacities of CD70+ human cells: LB39-MEL CD70− clone (blue), LB39-MEL CD70+ clone (red) and LB1319-MEL cells (black). Results ( A , B , D and E ) are expressed as mean values ( n =7). P -values were evaluated using the Tukey ANOVA test. * P <0.05; ** P <0.005; *** P <0.001.
    Figure Legend Snippet: In melanoma cells CD70 expression is associated with decreased in vitro migration and invasion capacities. Decreased in vitro migration ( A ) and invasion ( B ) capacities of B16F10-CD70+ cells analysed using, respectively, transwells and invasion chambers with B16F10-wt vs B16F10-CD70 cells. In the upper chamber the medium was or not (NT) supplemented with Ctrl Ig (Ct) or anti-CD70 mAb (QA). Decreased in vitro migration capacity of B16F10-CD70+ cells was analysed by wound-healing experiments done with B16F10-wt and B16F10-CD70 cells ( C ). Decreased in vitro migration ( D ) and invasion ( E ) capacities of CD70+ human cells: LB39-MEL CD70− clone (blue), LB39-MEL CD70+ clone (red) and LB1319-MEL cells (black). Results ( A , B , D and E ) are expressed as mean values ( n =7). P -values were evaluated using the Tukey ANOVA test. * P <0.05; ** P <0.005; *** P <0.001.

    Techniques Used: Expressing, In Vitro, Migration

    CD70 signalling modulates MAPK activation, RhoE and ROCK1 expression, and cytoskeleton formation. Western blot analysis ( A ) and quantification ( B ) in arbitrary units (a.u.) of CD70 M decrease and CD70 T increase in LB1319-MEL cells incubated with anti-CD70 mAb (QA). Controls are untreated (NT) and Ctrl Ig (Ct)-incubated LB1319-MEL cells. ( C ) Western blots analyses of the MEK/ERK pathway activation and RhoE overexpression in these LB1319-MEL cells. ( D ) The 3D invasion using LB1319-MEL spheroids treated with the Abs plus MEK inhibitor U0126, showing anti-CD70-induced invasion enhancement and U0126-induced invasion inhibition. ( E ) Western blot quantification of total ROCK1 and ROCK2 in LB1319-MEL cells incubated with Ctrl or anti-CD70 mAb, showing anti-CD70-induced ROCK1 inhibition. ( F ) Disappearance of stress fibres (actin) and focal adhesions (vinculin) in LB1319-MEL cells treated with anti-CD70 mAb for 48 h. Results in B and E are expressed as mean values ( n =3). P -values were evaluated using the Tukey ANOVA test ( B ) or t -test ( E ). ** P <0.005.
    Figure Legend Snippet: CD70 signalling modulates MAPK activation, RhoE and ROCK1 expression, and cytoskeleton formation. Western blot analysis ( A ) and quantification ( B ) in arbitrary units (a.u.) of CD70 M decrease and CD70 T increase in LB1319-MEL cells incubated with anti-CD70 mAb (QA). Controls are untreated (NT) and Ctrl Ig (Ct)-incubated LB1319-MEL cells. ( C ) Western blots analyses of the MEK/ERK pathway activation and RhoE overexpression in these LB1319-MEL cells. ( D ) The 3D invasion using LB1319-MEL spheroids treated with the Abs plus MEK inhibitor U0126, showing anti-CD70-induced invasion enhancement and U0126-induced invasion inhibition. ( E ) Western blot quantification of total ROCK1 and ROCK2 in LB1319-MEL cells incubated with Ctrl or anti-CD70 mAb, showing anti-CD70-induced ROCK1 inhibition. ( F ) Disappearance of stress fibres (actin) and focal adhesions (vinculin) in LB1319-MEL cells treated with anti-CD70 mAb for 48 h. Results in B and E are expressed as mean values ( n =3). P -values were evaluated using the Tukey ANOVA test ( B ) or t -test ( E ). ** P <0.005.

    Techniques Used: Activation Assay, Expressing, Western Blot, Incubation, Over Expression, Inhibition

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    ATCC b16f10 cd70
    <t>CD70</t> ectopic expression in human melanomas. Representative images of CD70 expression after staining by IHC paraffin-embedded melanoma human biopsies ( A ) or staining of cryostat sections of frozen melanoma tumour specimens ( B ). Negative staining corresponded to CD70 expression in <2% of melanoma cells, moderate staining in 2–20% and strong staining in more than 20%. ( C ) Three slides of the same melanoma fragment showing that CD70 expression is in the tumour cells. The slides were labelled with anti-CD70 mAb, anti-MelanA or anti-KBA.62 antitumour antibodies. ( D ) Primary human melanomas contain more CD70+ tumour cells than metastases as quantified in paraffin-embedded melanoma biopsies. ( E ) The intensity of CD70 expression is also stronger in primary melanomas than in metastases. P -values were evaluated using the Mann–Whitney test ( D ) or the χ 2 -test ( E ). ** P <0.005; *** P <0.001.
    B16f10 Cd70, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/b16f10 cd70/product/ATCC
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    b16f10 cd70 - by Bioz Stars, 2024-04
    93/100 stars
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    CD70 ectopic expression in human melanomas. Representative images of CD70 expression after staining by IHC paraffin-embedded melanoma human biopsies ( A ) or staining of cryostat sections of frozen melanoma tumour specimens ( B ). Negative staining corresponded to CD70 expression in <2% of melanoma cells, moderate staining in 2–20% and strong staining in more than 20%. ( C ) Three slides of the same melanoma fragment showing that CD70 expression is in the tumour cells. The slides were labelled with anti-CD70 mAb, anti-MelanA or anti-KBA.62 antitumour antibodies. ( D ) Primary human melanomas contain more CD70+ tumour cells than metastases as quantified in paraffin-embedded melanoma biopsies. ( E ) The intensity of CD70 expression is also stronger in primary melanomas than in metastases. P -values were evaluated using the Mann–Whitney test ( D ) or the χ 2 -test ( E ). ** P <0.005; *** P <0.001.

    Journal: British Journal of Cancer

    Article Title: Melanoma-expressed CD70 is involved in invasion and metastasis

    doi: 10.1038/bjc.2015.412

    Figure Lengend Snippet: CD70 ectopic expression in human melanomas. Representative images of CD70 expression after staining by IHC paraffin-embedded melanoma human biopsies ( A ) or staining of cryostat sections of frozen melanoma tumour specimens ( B ). Negative staining corresponded to CD70 expression in <2% of melanoma cells, moderate staining in 2–20% and strong staining in more than 20%. ( C ) Three slides of the same melanoma fragment showing that CD70 expression is in the tumour cells. The slides were labelled with anti-CD70 mAb, anti-MelanA or anti-KBA.62 antitumour antibodies. ( D ) Primary human melanomas contain more CD70+ tumour cells than metastases as quantified in paraffin-embedded melanoma biopsies. ( E ) The intensity of CD70 expression is also stronger in primary melanomas than in metastases. P -values were evaluated using the Mann–Whitney test ( D ) or the χ 2 -test ( E ). ** P <0.005; *** P <0.001.

    Article Snippet: B16F10-CD70 and its control cell line B16F10-wt were produced in our laboratory ( Cormary et al , 2005 ) by genetic modification of B16F10 murine melanoma cells purchased from the ATCC.

    Techniques: Expressing, Staining, Negative Staining, MANN-WHITNEY

    CD70 expression decreases with time and disease progression in human melanomas. ( A ) CD70 membrane expression measured by flow cytometry in three representative melanoma cell lines: LB1319-MEL; LB39-MEL; and LB39-MEL clones CD70+ vs CD70−. ( B ) Decrease of membrane CD70 expression in three melanoma cell lines derived from the same patient: LB33-MEL.A (skin, 1988); LB33-MEL.B (lymph node, 1993); and LB33-MEL.D (intestine, 1999). ( C ) Decrease with disease progression of melanoma-expressed CD70: primary tumours, skin and lymph node metastases from the same patient C121084. ( D ) Monomeric (CD70 M) and trimeric (CD70 T) forms of CD70 detected by western blot in LB1319-MEL (total, cytosolic and membrane fractions), B16F10-wt and B16F10-CD70 cells.

    Journal: British Journal of Cancer

    Article Title: Melanoma-expressed CD70 is involved in invasion and metastasis

    doi: 10.1038/bjc.2015.412

    Figure Lengend Snippet: CD70 expression decreases with time and disease progression in human melanomas. ( A ) CD70 membrane expression measured by flow cytometry in three representative melanoma cell lines: LB1319-MEL; LB39-MEL; and LB39-MEL clones CD70+ vs CD70−. ( B ) Decrease of membrane CD70 expression in three melanoma cell lines derived from the same patient: LB33-MEL.A (skin, 1988); LB33-MEL.B (lymph node, 1993); and LB33-MEL.D (intestine, 1999). ( C ) Decrease with disease progression of melanoma-expressed CD70: primary tumours, skin and lymph node metastases from the same patient C121084. ( D ) Monomeric (CD70 M) and trimeric (CD70 T) forms of CD70 detected by western blot in LB1319-MEL (total, cytosolic and membrane fractions), B16F10-wt and B16F10-CD70 cells.

    Article Snippet: B16F10-CD70 and its control cell line B16F10-wt were produced in our laboratory ( Cormary et al , 2005 ) by genetic modification of B16F10 murine melanoma cells purchased from the ATCC.

    Techniques: Expressing, Flow Cytometry, Clone Assay, Derivative Assay, Western Blot

    CD70 expression is associated with decreased in vivo metastatic capacity. ( A ) C57BL/6, C57BL/6 IFN- γ KO and NMRI nu/nu mice were injected i.v. with B16F10-wt (blue) or B16F10-CD70 (red) cells and pulmonary metastases were quantified, showing that B16F10-CD70 cells induced less metastases implantations. ( B ) Representative lung photomicrographs (left) and enlargement of metastatic areas (right) of C57BL/6 mice injected i.v. with B16F10-wt (top) or B16F10-CD70 (bottom) cells. Arrows indicate metastases. P -values were evaluated using the t -test. *** P <0.001.

    Journal: British Journal of Cancer

    Article Title: Melanoma-expressed CD70 is involved in invasion and metastasis

    doi: 10.1038/bjc.2015.412

    Figure Lengend Snippet: CD70 expression is associated with decreased in vivo metastatic capacity. ( A ) C57BL/6, C57BL/6 IFN- γ KO and NMRI nu/nu mice were injected i.v. with B16F10-wt (blue) or B16F10-CD70 (red) cells and pulmonary metastases were quantified, showing that B16F10-CD70 cells induced less metastases implantations. ( B ) Representative lung photomicrographs (left) and enlargement of metastatic areas (right) of C57BL/6 mice injected i.v. with B16F10-wt (top) or B16F10-CD70 (bottom) cells. Arrows indicate metastases. P -values were evaluated using the t -test. *** P <0.001.

    Article Snippet: B16F10-CD70 and its control cell line B16F10-wt were produced in our laboratory ( Cormary et al , 2005 ) by genetic modification of B16F10 murine melanoma cells purchased from the ATCC.

    Techniques: Expressing, In Vivo, Injection

    In melanoma cells CD70 expression is associated with decreased in vitro migration and invasion capacities. Decreased in vitro migration ( A ) and invasion ( B ) capacities of B16F10-CD70+ cells analysed using, respectively, transwells and invasion chambers with B16F10-wt vs B16F10-CD70 cells. In the upper chamber the medium was or not (NT) supplemented with Ctrl Ig (Ct) or anti-CD70 mAb (QA). Decreased in vitro migration capacity of B16F10-CD70+ cells was analysed by wound-healing experiments done with B16F10-wt and B16F10-CD70 cells ( C ). Decreased in vitro migration ( D ) and invasion ( E ) capacities of CD70+ human cells: LB39-MEL CD70− clone (blue), LB39-MEL CD70+ clone (red) and LB1319-MEL cells (black). Results ( A , B , D and E ) are expressed as mean values ( n =7). P -values were evaluated using the Tukey ANOVA test. * P <0.05; ** P <0.005; *** P <0.001.

    Journal: British Journal of Cancer

    Article Title: Melanoma-expressed CD70 is involved in invasion and metastasis

    doi: 10.1038/bjc.2015.412

    Figure Lengend Snippet: In melanoma cells CD70 expression is associated with decreased in vitro migration and invasion capacities. Decreased in vitro migration ( A ) and invasion ( B ) capacities of B16F10-CD70+ cells analysed using, respectively, transwells and invasion chambers with B16F10-wt vs B16F10-CD70 cells. In the upper chamber the medium was or not (NT) supplemented with Ctrl Ig (Ct) or anti-CD70 mAb (QA). Decreased in vitro migration capacity of B16F10-CD70+ cells was analysed by wound-healing experiments done with B16F10-wt and B16F10-CD70 cells ( C ). Decreased in vitro migration ( D ) and invasion ( E ) capacities of CD70+ human cells: LB39-MEL CD70− clone (blue), LB39-MEL CD70+ clone (red) and LB1319-MEL cells (black). Results ( A , B , D and E ) are expressed as mean values ( n =7). P -values were evaluated using the Tukey ANOVA test. * P <0.05; ** P <0.005; *** P <0.001.

    Article Snippet: B16F10-CD70 and its control cell line B16F10-wt were produced in our laboratory ( Cormary et al , 2005 ) by genetic modification of B16F10 murine melanoma cells purchased from the ATCC.

    Techniques: Expressing, In Vitro, Migration

    CD70 signalling modulates MAPK activation, RhoE and ROCK1 expression, and cytoskeleton formation. Western blot analysis ( A ) and quantification ( B ) in arbitrary units (a.u.) of CD70 M decrease and CD70 T increase in LB1319-MEL cells incubated with anti-CD70 mAb (QA). Controls are untreated (NT) and Ctrl Ig (Ct)-incubated LB1319-MEL cells. ( C ) Western blots analyses of the MEK/ERK pathway activation and RhoE overexpression in these LB1319-MEL cells. ( D ) The 3D invasion using LB1319-MEL spheroids treated with the Abs plus MEK inhibitor U0126, showing anti-CD70-induced invasion enhancement and U0126-induced invasion inhibition. ( E ) Western blot quantification of total ROCK1 and ROCK2 in LB1319-MEL cells incubated with Ctrl or anti-CD70 mAb, showing anti-CD70-induced ROCK1 inhibition. ( F ) Disappearance of stress fibres (actin) and focal adhesions (vinculin) in LB1319-MEL cells treated with anti-CD70 mAb for 48 h. Results in B and E are expressed as mean values ( n =3). P -values were evaluated using the Tukey ANOVA test ( B ) or t -test ( E ). ** P <0.005.

    Journal: British Journal of Cancer

    Article Title: Melanoma-expressed CD70 is involved in invasion and metastasis

    doi: 10.1038/bjc.2015.412

    Figure Lengend Snippet: CD70 signalling modulates MAPK activation, RhoE and ROCK1 expression, and cytoskeleton formation. Western blot analysis ( A ) and quantification ( B ) in arbitrary units (a.u.) of CD70 M decrease and CD70 T increase in LB1319-MEL cells incubated with anti-CD70 mAb (QA). Controls are untreated (NT) and Ctrl Ig (Ct)-incubated LB1319-MEL cells. ( C ) Western blots analyses of the MEK/ERK pathway activation and RhoE overexpression in these LB1319-MEL cells. ( D ) The 3D invasion using LB1319-MEL spheroids treated with the Abs plus MEK inhibitor U0126, showing anti-CD70-induced invasion enhancement and U0126-induced invasion inhibition. ( E ) Western blot quantification of total ROCK1 and ROCK2 in LB1319-MEL cells incubated with Ctrl or anti-CD70 mAb, showing anti-CD70-induced ROCK1 inhibition. ( F ) Disappearance of stress fibres (actin) and focal adhesions (vinculin) in LB1319-MEL cells treated with anti-CD70 mAb for 48 h. Results in B and E are expressed as mean values ( n =3). P -values were evaluated using the Tukey ANOVA test ( B ) or t -test ( E ). ** P <0.005.

    Article Snippet: B16F10-CD70 and its control cell line B16F10-wt were produced in our laboratory ( Cormary et al , 2005 ) by genetic modification of B16F10 murine melanoma cells purchased from the ATCC.

    Techniques: Activation Assay, Expressing, Western Blot, Incubation, Over Expression, Inhibition